Rh4DG311100

Plectin/S10 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001582
Physical Location & Seq
Reverse (-)
53370907 .. 53374635
3729 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG311100.1

Sequence Viewer

Length: 435 bp
ATGCACTATTACTGGTACCTCACCAATGATGGCATTGAGTTCTTGAGGAACTACCTCAACCTGCCCTCGGAGATTGTGCCCAACACTCTCAAGAAGCAGGCTAAGCCCGCCGGCCGTCCTTTCGGCGGCCCACCAGGCGACCGCCCTCGTGGCCCACCTCGCTTTGGTGATGGAGAGCGCAGATTTGGAGGTGACCGTGATGGTTACCGTGGAGGTCCTCGTGGTGGACCAGGAGGTGACTTTGGCAGGGACAAGGGAGGAGCCCCTGCTGACTACAGACCTTCTTTCGGGATTTTGATATGCGGCAAGTTGAGGGGGAAACACGTGTCGGAGGAGAATGGAGACCCCTACAAGGCATCAATTCCATTGATTCAGGCCACAAGCCCAGAAGCAGATGTTTTACTTGCTTATGAGATGAATGGAGAGGAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
S10_plectin

Protein Analysis

144

Amino Acids

15.7

Weight (kDa)

7.83

Isoelectric Point (pI)

45.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S10_plectin PF03501 2 - 33 2.3e-12 Plectin/S10 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 69
Acc65I GGTACC 1 cut(s) 15
AccB1I GGYRCC 1 cut(s) 15
AciI CCGC 4 cut(s) 108, 126, 142, 303
AcoI YGGCCR 1 cut(s) 112
AcvI CACGTG 1 cut(s) 325
AfaI GTAC 1 cut(s) 17
AfiI CCNNNNNNNGG 6 cut(s) 67, 125, 164, 224, 287, 352
AflIII ACRYGT 2 cut(s) 322, 324
AjnI CCWGG 2 cut(s) 133, 229
Alw26I GTCTC 1 cut(s) 336
AoxI GGCC 4 cut(s) 112, 127, 151, 375
Asp718I GGTACC 1 cut(s) 15
AspLEI GCGC 1 cut(s) 180
AspS9I GGNCC 4 cut(s) 128, 152, 215, 227
AsuHPI GGTGA 4 cut(s) 13, 179, 203, 248
AvaII GGWCC 2 cut(s) 215, 227
BaeGI GKGCMC 1 cut(s) 81
BanI GGYRCC 1 cut(s) 15
BanII GRGCYC 1 cut(s) 265
BauI CACGAG 2 cut(s) 147, 219
BbrPI CACGTG 1 cut(s) 325
BccI CCATC 3 cut(s) 23, 164, 194
BceAI ACGGC 1 cut(s) 99
BciT130I CCWGG 2 cut(s) 135, 231
BcoDI GTCTC 1 cut(s) 336
BfmI CTRYAG 1 cut(s) 274
BfuAI ACCTGC 1 cut(s) 69
BglI GCCNNNNNGGC 2 cut(s) 135, 150
BisI GCNGC 2 cut(s) 127, 304
BlpI GCTNAGC 1 cut(s) 102
BlsI GCNGC 2 cut(s) 128, 305
Bme1390I CCNGG 2 cut(s) 135, 231
Bme18I GGWCC 2 cut(s) 215, 227
BmgT120I GGNCC 4 cut(s) 128, 152, 215, 227
BmiI GGNNCC 2 cut(s) 17, 262
BmrFI CCNGG 2 cut(s) 135, 231
BmsI GCATC 1 cut(s) 365
Bpu1102I GCTNAGC 1 cut(s) 102
BpuEI CTTGAG 2 cut(s) 64, 74
BsaAI YACGTR 1 cut(s) 325
BsaI GGTCTC 1 cut(s) 336
BsaJI CCNNGG 2 cut(s) 66, 208
Bsc4I CCNNNNNNNGG 6 cut(s) 67, 125, 164, 224, 287, 352
Bse118I RCCGGY 1 cut(s) 110
Bse1I ACTGG 1 cut(s) 17
BseBI CCWGG 2 cut(s) 135, 231
BseDI CCNNGG 2 cut(s) 66, 208
BseLI CCNNNNNNNGG 6 cut(s) 67, 125, 164, 224, 287, 352
BseNI ACTGG 1 cut(s) 17
BseRI GAGGAG 2 cut(s) 273, 347
BseSI GKGCMC 1 cut(s) 81
BseX3I CGGCCG 1 cut(s) 112
Bsh1285I CGRYCG 2 cut(s) 115, 142
BshFI GGCC 4 cut(s) 114, 129, 153, 377
BshNI GGYRCC 1 cut(s) 15
BsiEI CGRYCG 2 cut(s) 115, 142
BsiSI CCGG 1 cut(s) 111
BslFI GGGAC 1 cut(s) 263
BslI CCNNNNNNNGG 6 cut(s) 67, 125, 164, 224, 287, 352
BsmAI GTCTC 1 cut(s) 336
BsmFI GGGAC 1 cut(s) 263
BsnI GGCC 4 cut(s) 114, 129, 153, 377
Bso31I GGTCTC 1 cut(s) 336
Bsp1286I GDGCHC 2 cut(s) 81, 265
Bsp1720I GCTNAGC 1 cut(s) 102
BspACI CCGC 4 cut(s) 108, 126, 142, 303
BspANI GGCC 4 cut(s) 114, 129, 153, 377
BspLI GGNNCC 2 cut(s) 17, 262
BspMI ACCTGC 1 cut(s) 69
BspT107I GGYRCC 1 cut(s) 15
BspTNI GGTCTC 1 cut(s) 336
BsrFI RCCGGY 1 cut(s) 110
BsrI ACTGG 1 cut(s) 17
BssAI RCCGGY 1 cut(s) 110
BssECI CCNNGG 2 cut(s) 66, 208
BssSI CACGAG 2 cut(s) 147, 219
Bst2BI CACGAG 2 cut(s) 147, 219
Bst2UI CCWGG 2 cut(s) 135, 231
Bst4CI ACNGT 2 cut(s) 197, 209
BstBAI YACGTR 1 cut(s) 325
BstC8I GCNNGC 3 cut(s) 99, 108, 112
BstDEI CTNAG 1 cut(s) 102
BstDSI CCRYGG 1 cut(s) 208
BstEII GGTNACC 2 cut(s) 191, 203
BstHHI GCGC 1 cut(s) 180
BstMAI GTCTC 1 cut(s) 336
BstMCI CGRYCG 2 cut(s) 115, 142
BstMWI GCNNNNNNNGC 5 cut(s) 103, 107, 135, 150, 159
BstNI CCWGG 2 cut(s) 135, 231
BstPI GGTNACC 2 cut(s) 191, 203
BstSCI CCNGG 2 cut(s) 133, 229
BstSFI CTRYAG 1 cut(s) 274
BstSLI GKGCMC 1 cut(s) 81
BstZI CGGCCG 1 cut(s) 112
BsuRI GGCC 4 cut(s) 114, 129, 153, 377
BtgI CCRYGG 1 cut(s) 208
BveI ACCTGC 1 cut(s) 69
Cac8I GCNNGC 3 cut(s) 99, 108, 112
CfoI GCGC 1 cut(s) 180
Cfr10I RCCGGY 1 cut(s) 110
Cfr13I GGNCC 4 cut(s) 128, 152, 215, 227
Csp6I GTAC 1 cut(s) 16
CviJI RGCY 8 cut(s) 101, 106, 114, 129, 153, 263, 377, 384
CviKI_1 RGCY 8 cut(s) 101, 106, 114, 129, 153, 263, 377, 384
CviQI GTAC 1 cut(s) 16
DdeI CTNAG 1 cut(s) 102
EaeI YGGCCR 1 cut(s) 112
EagI CGGCCG 1 cut(s) 112
EclXI CGGCCG 1 cut(s) 112
Eco24I GRGCYC 1 cut(s) 265
Eco31I GGTCTC 1 cut(s) 336
Eco47I GGWCC 2 cut(s) 215, 227
Eco52I CGGCCG 1 cut(s) 112
Eco72I CACGTG 1 cut(s) 325
Eco91I GGTNACC 2 cut(s) 191, 203
EcoO109I RGGNCCY 1 cut(s) 215
EcoO65I GGTNACC 2 cut(s) 191, 203
EcoRII CCWGG 2 cut(s) 133, 229
EcoT38I GRGCYC 1 cut(s) 265
FaiI YATR 2 cut(s) 301, 411
FaqI GGGAC 1 cut(s) 263
FauI CCCGC 1 cut(s) 115
Fnu4HI GCNGC 2 cut(s) 127, 304
FriOI GRGCYC 1 cut(s) 265
Fsp4HI GCNGC 2 cut(s) 127, 304
GlaI GCGC 1 cut(s) 179
GluI GCNGC 2 cut(s) 127, 304
HaeIII GGCC 4 cut(s) 114, 129, 153, 377
HapII CCGG 1 cut(s) 111
HhaI GCGC 1 cut(s) 180
Hin6I GCGC 1 cut(s) 178
HinP1I GCGC 1 cut(s) 178
HinfI GANTC 1 cut(s) 370
HpaII CCGG 1 cut(s) 111
HphI GGTGA 4 cut(s) 13, 179, 203, 248
Hpy166II GTNNAC 1 cut(s) 227
Hpy188I TCNGA 2 cut(s) 70, 331
Hpy188III TCNNGA 3 cut(s) 43, 91, 289
Hpy8I GTNNAC 1 cut(s) 227
HpyAV CCTTC 1 cut(s) 291
HpyCH4III ACNGT 2 cut(s) 197, 209
HpyCH4IV ACGT 1 cut(s) 324
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 5 cut(s) 103, 107, 135, 150, 159
HpyF3I CTNAG 1 cut(s) 102
HpySE526I ACGT 1 cut(s) 324
HspAI GCGC 1 cut(s) 178
KpnI GGTACC 1 cut(s) 19
KroI GCCGGC 1 cut(s) 110
KroNI GCCGGC 1 cut(s) 112
LmnI GCTCC 1 cut(s) 260
LweI GCATC 1 cut(s) 365
MaeII ACGT 1 cut(s) 324
MaeIII GTNAC 3 cut(s) 191, 203, 236
MhlI GDGCHC 2 cut(s) 81, 265
MluCI AATT 1 cut(s) 360
MmeI TCCRAC 1 cut(s) 309
MroNI GCCGGC 1 cut(s) 110
MspI CCGG 1 cut(s) 111
MspR9I CCNGG 2 cut(s) 135, 231
MvaI CCWGG 2 cut(s) 135, 231
MwoI GCNNNNNNNGC 5 cut(s) 103, 107, 135, 150, 159
NaeI GCCGGC 1 cut(s) 112
NgoMIV GCCGGC 1 cut(s) 110
NlaIV GGNNCC 2 cut(s) 17, 262
NmuCI GTSAC 2 cut(s) 191, 236
PdiI GCCGGC 1 cut(s) 112
PfeI GAWTC 1 cut(s) 370
PkrI GCNGC 2 cut(s) 128, 305
PmaCI CACGTG 1 cut(s) 325
PmlI CACGTG 1 cut(s) 325
Ppu21I YACGTR 1 cut(s) 325
PpuMI RGGWCCY 1 cut(s) 215
Psp5II RGGWCCY 1 cut(s) 215
Psp6I CCWGG 2 cut(s) 133, 229
PspCI CACGTG 1 cut(s) 325
PspEI GGTNACC 2 cut(s) 191, 203
PspGI CCWGG 2 cut(s) 133, 229
PspN4I GGNNCC 2 cut(s) 17, 262
PspPI GGNCC 4 cut(s) 128, 152, 215, 227
PspPPI RGGWCCY 1 cut(s) 215
RsaI GTAC 1 cut(s) 17
RsaNI GTAC 1 cut(s) 16
SatI GCNGC 2 cut(s) 127, 304
Sau96I GGNCC 4 cut(s) 128, 152, 215, 227
ScrFI CCNGG 2 cut(s) 135, 231
SduI GDGCHC 2 cut(s) 81, 265
SetI ASST 9 cut(s) 21, 57, 63, 160, 193, 217, 238, 283, 327
SfaNI GCATC 1 cut(s) 365
SfcI CTRYAG 1 cut(s) 274
SinI GGWCC 2 cut(s) 215, 227
SmlI CTYRAG 2 cut(s) 43, 89
SmoI CTYRAG 2 cut(s) 43, 89
Sse9I AATT 1 cut(s) 360
SsiI CCGC 4 cut(s) 108, 126, 142, 303
StyD4I CCNGG 2 cut(s) 133, 229
TaaI ACNGT 2 cut(s) 197, 209
TaiI ACGT 1 cut(s) 327
TasI AATT 1 cut(s) 360
TauI GCSGC 2 cut(s) 129, 306
TfiI GAWTC 1 cut(s) 370
TseFI GTSAC 2 cut(s) 191, 236
Tsp45I GTSAC 2 cut(s) 191, 236
TspDTI ATGAA 1 cut(s) 431
VpaK11BI GGWCC 2 cut(s) 215, 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.