Rh5BG246300

Belongs to the universal ribosomal protein uS4 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001270
Physical Location & Seq
Forward (+)
30020328 .. 30028638
8311 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG246300.1

Sequence Viewer

Length: 516 bp
ATGTCACTGGACTACGACATTTGGCAGTCGACTGTTGAACTTTCTTCACAGCTTGAAGCTTTTGCCAAAACCAGACTGGTGCTCTATTTTGCAGGTATGCAACTCGTGGATCTGGAGATAATGAAAATCCAAATGGTCTCGGAGGAGATCAGCCATCGCTCGAGGAGCAGAGCACCTCGTCACTCCTCCGATATCTCCTCAATCCTTGACAAGCTCAAGAATTTCACTCAAAGACCACAAACCAAGCTTCGACGATGTTCAGAGAAGCCAAGACGTCCTGATGAGAAGGAACATTTGGATGTTGAGCTGAAGCTTGTTGGAGAATATGGGCTTCGGCGCAAGAAGGACTTGTGGAGGGTCCAGTATGCTTTGAGACGCATCTGTAATGCTGCTAGGGAACTTCTTACTCTGGACAACGTTGCTGAAACATCCAGTGGCTATGGGTTAATATGTGAAGGACTCGCAGTACTGGTGATGGATTTACTATACATGAGTCCTACAAATCCAGCAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S4 S4

Protein Analysis

171

Amino Acids

19.72

Weight (kDa)

8.77

Isoelectric Point (pI)

58.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 277
Acc36I ACCTGC 1 cut(s) 83
AccI GTMKAC 1 cut(s) 29
AclI AACGTT 1 cut(s) 417
AclWI GGATC 1 cut(s) 117
AcsI RAATTY 1 cut(s) 220
AcuI CTGAAG 1 cut(s) 329
AcyI GRCGYC 1 cut(s) 274
AfaI GTAC 1 cut(s) 468
AgsI TTSAA 2 cut(s) 38, 56
AjuI GAANNNNNNNTTGG 2 cut(s) 278, 310
AluBI AGCT 7 cut(s) 52, 59, 214, 247, 307, 313, 513
AluI AGCT 7 cut(s) 52, 59, 214, 247, 307, 313, 513
Alw21I GWGCWC 2 cut(s) 84, 175
Alw26I GTCTC 2 cut(s) 142, 367
AlwI GGATC 1 cut(s) 117
Ama87I CYCGRG 1 cut(s) 160
ApeKI GCWGC 1 cut(s) 389
ApoI RAATTY 1 cut(s) 220
AspLEI GCGC 1 cut(s) 339
AspS9I GGNCC 1 cut(s) 358
AsuHPI GGTGA 1 cut(s) 484
AvaI CYCGRG 1 cut(s) 160
AvaII GGWCC 1 cut(s) 358
BauI CACGAG 1 cut(s) 104
Bbv12I GWGCWC 2 cut(s) 84, 175
BbvI GCAGC 1 cut(s) 376
BccI CCATC 2 cut(s) 162, 469
BcoDI GTCTC 2 cut(s) 142, 367
BfaI CTAG 1 cut(s) 393
BfuAI ACCTGC 1 cut(s) 83
BisI GCNGC 1 cut(s) 390
BlsI GCNGC 1 cut(s) 391
BmcAI AGTACT 1 cut(s) 468
Bme18I GGWCC 1 cut(s) 358
BmeT110I CYCGRG 1 cut(s) 160
BmgT120I GGNCC 1 cut(s) 358
BmiI GGNNCC 1 cut(s) 359
BmsI GCATC 1 cut(s) 387
BpmI CTGGAG 1 cut(s) 134
BpuEI CTTGAG 1 cut(s) 200
BsaHI GRCGYC 1 cut(s) 274
BsaI GGTCTC 1 cut(s) 142
Bse1I ACTGG 5 cut(s) 12, 81, 361, 432, 474
BseGI GGATG 2 cut(s) 304, 428
BseNI ACTGG 5 cut(s) 12, 81, 361, 432, 474
BseRI GAGGAG 4 cut(s) 158, 175, 178, 187
BseXI GCAGC 1 cut(s) 376
BsiHKAI GWGCWC 2 cut(s) 84, 175
BsiHKCI CYCGRG 1 cut(s) 160
BsmAI GTCTC 2 cut(s) 142, 367
BsmBI CGTCTC 1 cut(s) 367
Bso31I GGTCTC 1 cut(s) 142
BsoBI CYCGRG 1 cut(s) 160
Bsp1286I GDGCHC 2 cut(s) 84, 175
Bsp143I GATC 2 cut(s) 109, 147
BspLI GGNNCC 1 cut(s) 359
BspMI ACCTGC 1 cut(s) 83
BspPI GGATC 1 cut(s) 117
BspTNI GGTCTC 1 cut(s) 142
BsrI ACTGG 5 cut(s) 12, 81, 361, 432, 474
BssMI GATC 2 cut(s) 109, 147
BssNI GRCGYC 1 cut(s) 274
BssSI CACGAG 1 cut(s) 104
Bst2BI CACGAG 1 cut(s) 104
Bst4CI ACNGT 1 cut(s) 34
BstACI GRCGYC 1 cut(s) 274
BstC8I GCNNGC 1 cut(s) 511
BstF5I GGATG 2 cut(s) 304, 428
BstHHI GCGC 1 cut(s) 339
BstKTI GATC 2 cut(s) 112, 150
BstMAI GTCTC 2 cut(s) 142, 367
BstMBI GATC 2 cut(s) 109, 147
BstMWI GCNNNNNNNGC 1 cut(s) 165
BstV1I GCAGC 1 cut(s) 376
BstX2I RGATCY 1 cut(s) 109
BstYI RGATCY 1 cut(s) 109
BtgZI GCGATG 1 cut(s) 140
BtsCI GGATG 2 cut(s) 304, 428
BtsIMutI CAGTG 2 cut(s) 5, 439
BveI ACCTGC 1 cut(s) 83
Cac8I GCNNGC 1 cut(s) 511
CfoI GCGC 1 cut(s) 339
Cfr13I GGNCC 1 cut(s) 358
CseI GACGC 1 cut(s) 384
Csp6I GTAC 1 cut(s) 467
CviAII CATG 1 cut(s) 490
CviQI GTAC 1 cut(s) 467
DpnI GATC 2 cut(s) 111, 149
DpnII GATC 2 cut(s) 109, 147
Eco31I GGTCTC 1 cut(s) 142
Eco32I GATATC 1 cut(s) 193
Eco47I GGWCC 1 cut(s) 358
Eco57I CTGAAG 1 cut(s) 329
Eco88I CYCGRG 1 cut(s) 160
EcoRV GATATC 1 cut(s) 193
Esp3I CGTCTC 1 cut(s) 367
FaeI CATG 1 cut(s) 493
FaiI YATR 7 cut(s) 98, 327, 366, 441, 451, 487, 491
FalI AAGNNNNNCTT 2 cut(s) 332, 364
FatI CATG 1 cut(s) 489
FblI GTMKAC 1 cut(s) 29
Fnu4HI GCNGC 1 cut(s) 390
FokI GGATG 2 cut(s) 311, 415
Fsp4HI GCNGC 1 cut(s) 390
FspBI CTAG 1 cut(s) 393
GlaI GCGC 1 cut(s) 338
GluI GCNGC 1 cut(s) 390
GsuI CTGGAG 1 cut(s) 134
HgaI GACGC 1 cut(s) 384
HhaI GCGC 1 cut(s) 339
Hin1I GRCGYC 1 cut(s) 274
Hin1II CATG 1 cut(s) 493
Hin6I GCGC 1 cut(s) 337
HinP1I GCGC 1 cut(s) 337
HincII GTYRAC 1 cut(s) 30
HindII GTYRAC 1 cut(s) 30
HindIII AAGCTT 3 cut(s) 57, 245, 311
HinfI GANTC 2 cut(s) 459, 493
HphI GGTGA 1 cut(s) 484
Hpy166II GTNNAC 1 cut(s) 30
Hpy188I TCNGA 3 cut(s) 142, 190, 262
Hpy188III TCNNGA 4 cut(s) 113, 217, 278, 410
Hpy8I GTNNAC 1 cut(s) 30
Hpy99I CGWCG 1 cut(s) 255
HpyAV CCTTC 3 cut(s) 280, 337, 449
HpyCH4III ACNGT 1 cut(s) 34
HpyCH4IV ACGT 2 cut(s) 274, 417
HpyCH4V TGCA 2 cut(s) 92, 100
HpyF10VI GCNNNNNNNGC 1 cut(s) 165
HpySE526I ACGT 2 cut(s) 274, 417
Hsp92I GRCGYC 1 cut(s) 274
Hsp92II CATG 1 cut(s) 493
HspAI GCGC 1 cut(s) 337
Kzo9I GATC 2 cut(s) 109, 147
LmnI GCTCC 1 cut(s) 165
LpnPI CCDG 9 cut(s) 62, 78, 85, 98, 291, 374, 395, 445, 455
Lsp1109I GCAGC 1 cut(s) 376
LweI GCATC 1 cut(s) 387
MaeI CTAG 1 cut(s) 393
MaeII ACGT 2 cut(s) 274, 417
MaeIII GTNAC 2 cut(s) 3, 179
MalI GATC 2 cut(s) 111, 149
MboI GATC 2 cut(s) 109, 147
MboII GAAGA 1 cut(s) 36
MflI RGATCY 1 cut(s) 109
MhlI GDGCHC 2 cut(s) 84, 175
MluCI AATT 1 cut(s) 220
MlyI GAGTC 2 cut(s) 453, 502
MmeI TCCRAC 1 cut(s) 298
MnlI CCTC 6 cut(s) 136, 156, 186, 196, 208, 348
MseI TTAA 1 cut(s) 446
MslI CAYNNNNRTG 1 cut(s) 297
MwoI GCNNNNNNNGC 1 cut(s) 165
NdeII GATC 2 cut(s) 109, 147
NlaIII CATG 1 cut(s) 493
NlaIV GGNNCC 1 cut(s) 359
NmuCI GTSAC 2 cut(s) 3, 179
PaeR7I CTCGAG 1 cut(s) 160
PkrI GCNGC 1 cut(s) 391
PleI GAGTC 2 cut(s) 453, 501
PpsI GAGTC 2 cut(s) 453, 501
Psp1406I AACGTT 1 cut(s) 417
PspN4I GGNNCC 1 cut(s) 359
PspPI GGNCC 1 cut(s) 358
PspXI VCTCGAGB 1 cut(s) 160
PsuI RGATCY 1 cut(s) 109
RsaI GTAC 1 cut(s) 468
RsaNI GTAC 1 cut(s) 467
RseI CAYNNNNRTG 1 cut(s) 297
SalI GTCGAC 1 cut(s) 28
SaqAI TTAA 1 cut(s) 446
SatI GCNGC 1 cut(s) 390
Sau3AI GATC 2 cut(s) 109, 147
Sau96I GGNCC 1 cut(s) 358
ScaI AGTACT 1 cut(s) 468
SchI GAGTC 2 cut(s) 453, 502
SduI GDGCHC 2 cut(s) 84, 175
SfaNI GCATC 1 cut(s) 387
Sfr274I CTCGAG 1 cut(s) 160
SinI GGWCC 1 cut(s) 358
SlaI CTCGAG 1 cut(s) 160
SmiMI CAYNNNNRTG 1 cut(s) 297
SmlI CTYRAG 2 cut(s) 160, 215
SmoI CTYRAG 2 cut(s) 160, 215
Sse9I AATT 1 cut(s) 220
SspMI CTAG 1 cut(s) 393
TaaI ACNGT 1 cut(s) 34
TaiI ACGT 2 cut(s) 277, 420
TaqI TCGA 3 cut(s) 29, 161, 250
TasI AATT 1 cut(s) 220
TatI WGTACW 1 cut(s) 466
Tru1I TTAA 1 cut(s) 446
Tru9I TTAA 1 cut(s) 446
TscAI CASTG 2 cut(s) 12, 439
TseFI GTSAC 2 cut(s) 3, 179
TseI GCWGC 1 cut(s) 389
Tsp45I GTSAC 2 cut(s) 3, 179
TspDTI ATGAA 1 cut(s) 137
TspRI CASTG 2 cut(s) 12, 439
VpaK11BI GGWCC 1 cut(s) 358
XapI RAATTY 1 cut(s) 220
XcmI CCANNNNNNNNNTGG 1 cut(s) 73
XhoI CTCGAG 1 cut(s) 160
XmiI GTMKAC 1 cut(s) 29
XspI CTAG 1 cut(s) 393
ZraI GACGTC 1 cut(s) 275
ZrmI AGTACT 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.