AT1G02810

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000522
Physical Location & Seq
Forward (+)
618061 .. 620502
2442 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G02810.1

Sequence Viewer

Length: 1740 bp
ATGGAATCTCCAATATTCATTCTCATCACACTATCATTCTTTCTTCAATCAGTTCTTGCTTCTTCTCAAACTCTATCAAACTCTTCAACAATCTGCAAAACGACGCCGGATCCAAAGTATTGCAAATCCGTTTTTCCACATAGCCAAGGCAATGTTCAACAATACGGCTGTTTCTCTATCCGCAAATCGCTATCGCAATCGCGAAAATTTATTCGCACCGTCGATAGATATATCAAACGCAACGCTCATTTATCTCAACCTGCCGTTATCAGAGCTCTCCAAGATTGCCGTTTTCTCGCCGGTCTAACAATGGATTACCTCTTAACGTCGTTCGAAACCGTTAACGACACGTCAGCAAAAACCTCTTTCAAACCGCTGTCGTTTCCCAAAGCCGACGACATCCAAACGCTTCTTTCCGCGGCGCTGACTAATGAGCAGACGTGTCTTGAAGGACTTACCACGGCCGCTTCTTATTCCGCCACGTGGACTGTAAGAACCGGCGTCGCTTTGCCTCTCGTCAATGACACGAAGCTCTTGGGCGTCTCACTCGCTCTCTTCACCAAAGGTTGGGTTCCAAAGAAGAAGAAACGGGCCGGGTTTGCTTGGGCCCAACCGAGATCCGGTTCATCGACCCACACTAAACCATTCCGTCTGTTCCGTAACGGAGCTCTACCGTTAAAGATGACAGAGAAAACAAAAGCGGTTTACGAGTCACTCAGCAGAAGAAAACTCGCCGACGGTGACAGTAACGGTGATGGAGACGACGGAAGCATGGTTCTGATAAGCGATATCGTTACCGTGAGCCAAGACGGAACAGGAAATTTCACCAACATCACGGCAGCTGTAGCGGCGGCGCCGAACAACACCGACGGAAGCGCCGGTTTCTTTTTGATCTATGTGACGGCGGGAATCTACGAAGAGTATATCTCAATCGCGAAGAACAAAAGGTACATGATGATGATAGGTGACGGGATTAATCAGACGGTGGTCACCGGAAATAGAAGCGTCGTCGACGGTTGGACTACTTTCAATTCCGCCACATTTGCTGTGACAGCACCGAACTTTGTTGCGGTGAACATAACGTTCCGGAACACGGCCGGACCGGAGAAGCACCAGGCTGTTGCGTTACGGAGCGGCGCAGATTTCTCAATCTTCTATAGTTGTAGTTTCGAGGCTTATCAAGATACACTCTACACACATTCTCTAAGACAGTTTTATAGAGAATGCGATGTCTATGGAACGGTCGATTTTATATTTGGAAACGCGGCAGTTGTGTTTCAAAACTGTAATTTATACCCGAGGAAACCGATGCCAAATCAGTTCAATGCTATCACCGCCCAAGGCCGGTCTGATCCGAACCAGAACACTGGTACATCAATCCAAAACTGTACGATTAAGCCGGCGGATGATCTTGTTTCGAGTAACTATACAGTTAAAACGTATTTGGGTCGACCGTGGAAAGAGTATTCAAGGACGGTTTACATGCAATCGTACATTGATGGTTTCGTTGAACCGGTTGGTTGGAGAGAATGGAACGGTGATTTTGCGTTAAGTACATTGTACTATGCAGAGTATAACAATACCGGACCGGGCTCAAACACTACAAACCGGGTTACATGGCCTGGTTACCACGTGATTAATTCCACTGATGCAGCTAATTTCACGGTCACTGGTTTATTTATCGAAGCTGATTGGATTTGGAAGACAGGAGTGCCTTACACCAGCGGTCTAATTTCATAG

Protein Analysis

579

Amino Acids

63.95

Weight (kDa)

8.9

Isoelectric Point (pI)

29.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 29 - 185 3.8e-21 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 265 - 561 2.9e-138 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000522)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02810 AT2G47550 AT4G02330
fragaria_vesca FvH4_2g25960 FvH4_2g25960 FvH4_2g25970 FvH4_5g35570 FvH4_5g35580 FvH4_5g35590
malus_domestica MD03G1290900.v1.1 MD03G1291000.v1.1 MD08G1195600.v1.1 MD11G1307400.v1.1 MD11G1307500.v1.1
prunus_persica Prupe.1G529400_v2.0.a1 Prupe.1G529500_v2.0.a1 Prupe.8G263900_v2.0.a1 Prupe.8G264000_v2.0.a1
pyrus_communis pycom08g16800
rosa_chinensis RchiOBHm_Chr6g0294321 RchiOBHm_Chr6g0294331 RchiOBHm_Chr7g0236951 RchiOBHm_Chr7g0236971 RchiOBHm_Chr7g0237041 RchiOBHm_Chr7g0237051
rosa_laevigata RLG00000001034 RLG00000001035 RLG00000001039 RLG00000001043 RLG00000001045 RLG00000011880 RLG00000011881 RLG00000034181
rosa_multiflora Rmu_co8086188.1_g000001 Rmu_co8458485.1_g000001 Rmu_sc0000272.1_g000007 Rmu_sc0000272.1_g000009 Rmu_sc0002131.1_g000030 Rmu_sc0002131.1_g000037 Rmu_sc0005369.1_g000005 Rmu_sc0005369.1_g000008 Rmu_sc0007991.1_g000005 Rmu_sc0007991.1_g000013 Rmu_sc0033563.1_g000001
rosa_roxburghii Rroxscaffold_3G00224540 Rroxscaffold_3G00224600 Rroxscaffold_3G00224650 Rroxscaffold_3G00224660 Rroxscaffold_7G00173470 Rroxscaffold_7G00173480
rosa_rugosa Rorug06G0243400 Rorug06G0243500 Rorug07G0301200 Rorug07G0301500 Rorug07G0301600
rosa_samantha Rh6AG356000 Rh6AG356100 Rh6BG362600 Rh6BG362800 Rh6CG370000 Rh6CG370100 Rh6DG356500 Rh6DG356600 Rh7AG456200 Rh7AG456500 Rh7AG457500 Rh7AG457600 Rh7BG427500 Rh7BG427700 Rh7BG428400 Rh7BG428500 Rh7CG475100 Rh7CG475300 Rh7CG475700 Rh7CG475800 Rh7DG444100 Rh7DG444300 Rh7DG444700 Rh7DG444900
rosa_wichuraiana Rw6G031020 Rw6G031030 Rw7G037870 Rw7G037880 Rw7G037920 Rw7G037940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 268
AccB1I GGYRCC 1 cut(s) 853
AccB7I CCANNNNNTGG 1 cut(s) 567
AccBSI CCGCTC 1 cut(s) 1134
AccI GTMKAC 2 cut(s) 1011, 1450
AccII CGCG 4 cut(s) 202, 419, 935, 1265
AccIII TCCGGA 1 cut(s) 1086
AclI AACGTT 1 cut(s) 1082
AclWI GGATC 4 cut(s) 104, 117, 612, 1346
AcoI YGGCCR 2 cut(s) 462, 1095
AcsI RAATTY 2 cut(s) 206, 820
AcvI CACGTG 2 cut(s) 483, 1633
AcyI GRCGYC 4 cut(s) 104, 501, 540, 854
AfaI GTAC 6 cut(s) 950, 1372, 1390, 1493, 1555, 1562
AfiI CCNNNNNNNGG 5 cut(s) 483, 567, 620, 1093, 1344
AflIII ACRYGT 2 cut(s) 348, 440
AgeI ACCGGT 1 cut(s) 1513
AjiI CACGTC 2 cut(s) 351, 441
AjnI CCWGG 2 cut(s) 1113, 1621
AjuI GAANNNNNNNTTGG 2 cut(s) 138, 170
AloI GAACNNNNNNTCC 2 cut(s) 759, 791
AluBI AGCT 6 cut(s) 275, 532, 668, 842, 1655, 1688
AluI AGCT 6 cut(s) 275, 532, 668, 842, 1655, 1688
Alw21I GWGCWC 2 cut(s) 277, 670
Alw26I GTCTC 2 cut(s) 547, 753
AlwI GGATC 4 cut(s) 104, 117, 612, 1346
Ama87I CYCGRG 1 cut(s) 1297
Aor13HI TCCGGA 1 cut(s) 1086
AoxI GGCC 6 cut(s) 462, 591, 606, 1095, 1342, 1619
ApaI GGGCCC 1 cut(s) 610
ApeKI GCWGC 2 cut(s) 839, 1652
ApoI RAATTY 2 cut(s) 206, 820
AseI ATTAAT 2 cut(s) 975, 1638
AsiGI ACCGGT 1 cut(s) 1513
AspLEI GCGC 4 cut(s) 424, 856, 878, 1139
AspS9I GGNCC 5 cut(s) 591, 606, 607, 1100, 1586
AsuC2I CCSGG 3 cut(s) 595, 1590, 1610
AsuHPI GGTGA 9 cut(s) 550, 752, 764, 817, 977, 982, 1084, 1324, 1550
AsuII TTCGAA 1 cut(s) 333
AvaI CYCGRG 1 cut(s) 1297
AvaII GGWCC 2 cut(s) 1100, 1586
BaeGI GKGCMC 1 cut(s) 610
BamHI GGATCC 1 cut(s) 109
BanI GGYRCC 1 cut(s) 853
BanII GRGCYC 4 cut(s) 277, 610, 670, 1595
BbrPI CACGTG 2 cut(s) 483, 1633
BbsI GAAGAC 1 cut(s) 1709
Bbv12I GWGCWC 2 cut(s) 277, 670
BbvI GCAGC 2 cut(s) 851, 1664
BccI CCATC 2 cut(s) 749, 1493
BceAI ACGGC 7 cut(s) 181, 248, 273, 477, 852, 918, 1110
BciT130I CCWGG 2 cut(s) 1115, 1623
BcnI CCSGG 3 cut(s) 595, 1590, 1610
BcoDI GTCTC 2 cut(s) 547, 753
BfmI CTRYAG 2 cut(s) 843, 1156
BfoI RGCGCY 3 cut(s) 425, 857, 879
BfuAI ACCTGC 1 cut(s) 268
BisI GCNGC 8 cut(s) 420, 465, 840, 849, 852, 1135, 1266, 1653
BlsI GCNGC 8 cut(s) 421, 466, 841, 850, 853, 1136, 1267, 1654
Bme1390I CCNGG 5 cut(s) 595, 1115, 1590, 1610, 1623
Bme18I GGWCC 2 cut(s) 1100, 1586
BmeT110I CYCGRG 1 cut(s) 1297
BmgBI CACGTC 2 cut(s) 351, 441
BmgT120I GGNCC 5 cut(s) 591, 606, 607, 1100, 1586
BmiI GGNNCC 4 cut(s) 111, 573, 608, 855
BmrFI CCNGG 5 cut(s) 595, 1115, 1590, 1610, 1623
BmsI GCATC 2 cut(s) 1299, 1639
BoxI GACNNNNGTC 1 cut(s) 986
BpiI GAAGAC 1 cut(s) 1709
BplI GAGNNNNNCTC 2 cut(s) 911, 943
Bpu14I TTCGAA 1 cut(s) 333
BpuMI CCSGG 3 cut(s) 595, 1590, 1610
BsaAI YACGTR 2 cut(s) 483, 1633
BsaHI GRCGYC 4 cut(s) 104, 501, 540, 854
BsaJI CCNNGG 6 cut(s) 145, 417, 459, 1298, 1339, 1454
BsaWI WCCGGW 6 cut(s) 620, 992, 1086, 1102, 1513, 1583
Bsc4I CCNNNNNNNGG 5 cut(s) 483, 567, 620, 1093, 1344
Bse118I RCCGGY 6 cut(s) 299, 497, 878, 1344, 1399, 1513
Bse1I ACTGG 2 cut(s) 1372, 1675
Bse3DI GCAATG 1 cut(s) 157
BseAI TCCGGA 1 cut(s) 1086
BseBI CCWGG 2 cut(s) 1115, 1623
BseDI CCNNGG 6 cut(s) 145, 417, 459, 1298, 1339, 1454
BseGI GGATG 2 cut(s) 399, 1411
BseLI CCNNNNNNNGG 5 cut(s) 483, 567, 620, 1093, 1344
BseMI GCAATG 1 cut(s) 157
BseMII CTCAG 1 cut(s) 730
BseNI ACTGG 2 cut(s) 1372, 1675
BseSI GKGCMC 1 cut(s) 610
BseX3I CGGCCG 2 cut(s) 462, 1095
BseXI GCAGC 2 cut(s) 851, 1664
Bsh1236I CGCG 4 cut(s) 202, 419, 935, 1265
Bsh1285I CGRYCG 4 cut(s) 465, 1098, 1245, 1454
BshFI GGCC 6 cut(s) 464, 593, 608, 1097, 1344, 1621
BshNI GGYRCC 1 cut(s) 853
BshTI ACCGGT 1 cut(s) 1513
BsiEI CGRYCG 4 cut(s) 465, 1098, 1245, 1454
BsiHKAI GWGCWC 2 cut(s) 277, 670
BsiHKCI CYCGRG 1 cut(s) 1297
BslI CCNNNNNNNGG 5 cut(s) 483, 567, 620, 1093, 1344
BsmAI GTCTC 2 cut(s) 547, 753
BsmBI CGTCTC 2 cut(s) 547, 753
BsmI GAATGC 1 cut(s) 1229
BsnI GGCC 6 cut(s) 464, 593, 608, 1097, 1344, 1621
BsoBI CYCGRG 1 cut(s) 1297
Bsp119I TTCGAA 1 cut(s) 333
Bsp120I GGGCCC 1 cut(s) 606
Bsp1286I GDGCHC 4 cut(s) 277, 610, 670, 1595
Bsp13I TCCGGA 1 cut(s) 1086
Bsp143I GATC 5 cut(s) 109, 617, 891, 1351, 1408
Bsp68I TCGCGA 2 cut(s) 202, 935
BspANI GGCC 6 cut(s) 464, 593, 608, 1097, 1344, 1621
BspCNI CTCAG 1 cut(s) 729
BspEI TCCGGA 1 cut(s) 1086
BspFNI CGCG 4 cut(s) 202, 419, 935, 1265
BspLI GGNNCC 4 cut(s) 111, 573, 608, 855
BspMI ACCTGC 1 cut(s) 268
BspPI GGATC 4 cut(s) 104, 117, 612, 1346
BspT104I TTCGAA 1 cut(s) 333
BspT107I GGYRCC 1 cut(s) 853
BsrBI CCGCTC 1 cut(s) 1134
BsrDI GCAATG 1 cut(s) 157
BsrFI RCCGGY 6 cut(s) 299, 497, 878, 1344, 1399, 1513
BsrI ACTGG 2 cut(s) 1372, 1675
BssAI RCCGGY 6 cut(s) 299, 497, 878, 1344, 1399, 1513
BssECI CCNNGG 6 cut(s) 145, 417, 459, 1298, 1339, 1454
BssMI GATC 5 cut(s) 109, 617, 891, 1351, 1408
BssNI GRCGYC 4 cut(s) 104, 501, 540, 854
BssT1I CCWWGG 2 cut(s) 145, 1339
Bst2UI CCWGG 2 cut(s) 1115, 1623
Bst6I CTCTTC 3 cut(s) 88, 560, 912
BstACI GRCGYC 4 cut(s) 104, 501, 540, 854
BstBAI YACGTR 2 cut(s) 483, 1633
BstBI TTCGAA 1 cut(s) 333
BstC8I GCNNGC 1 cut(s) 1401
BstDEI CTNAG 2 cut(s) 716, 1205
BstDSI CCRYGG 3 cut(s) 417, 459, 1454
BstEII GGTNACC 2 cut(s) 988, 1625
BstF5I GGATG 2 cut(s) 399, 1411
BstFNI CGCG 4 cut(s) 202, 419, 935, 1265
BstH2I RGCGCY 3 cut(s) 425, 857, 879
BstHHI GCGC 4 cut(s) 424, 856, 878, 1139
BstKTI GATC 5 cut(s) 112, 620, 894, 1354, 1411
BstMAI GTCTC 2 cut(s) 547, 753
BstMBI GATC 5 cut(s) 109, 617, 891, 1351, 1408
BstMCI CGRYCG 4 cut(s) 465, 1098, 1245, 1454
BstMWI GCNNNNNNNGC 6 cut(s) 599, 845, 848, 1043, 1052, 1334
BstNI CCWGG 2 cut(s) 1115, 1623
BstNSI RCATGY 1 cut(s) 1486
BstPAI GACNNNNGTC 1 cut(s) 986
BstPI GGTNACC 2 cut(s) 988, 1625
BstSCI CCNGG 5 cut(s) 593, 1113, 1588, 1608, 1621
BstSFI CTRYAG 2 cut(s) 843, 1156
BstSLI GKGCMC 1 cut(s) 610
BstUI CGCG 4 cut(s) 202, 419, 935, 1265
BstV1I GCAGC 2 cut(s) 851, 1664
BstV2I GAAGAC 1 cut(s) 1709
BstX2I RGATCY 2 cut(s) 109, 617
BstXI CCANNNNNNTGG 1 cut(s) 1367
BstYI RGATCY 2 cut(s) 109, 617
BstZI CGGCCG 2 cut(s) 462, 1095
BsuRI GGCC 6 cut(s) 464, 593, 608, 1097, 1344, 1621
BtgI CCRYGG 3 cut(s) 417, 459, 1454
BtgZI GCGATG 1 cut(s) 1242
BtrI CACGTC 2 cut(s) 351, 441
BtsCI GGATG 2 cut(s) 399, 1411
BtsIMutI CAGTG 3 cut(s) 1365, 1644, 1668
BtuMI TCGCGA 2 cut(s) 202, 935
BveI ACCTGC 1 cut(s) 268
Cac8I GCNNGC 1 cut(s) 1401
CfoI GCGC 4 cut(s) 424, 856, 878, 1139
Cfr10I RCCGGY 6 cut(s) 299, 497, 878, 1344, 1399, 1513
Cfr13I GGNCC 5 cut(s) 591, 606, 607, 1100, 1586
Cfr42I CCGCGG 1 cut(s) 420
CpoI CGGWCCG 2 cut(s) 1100, 1586
CseI GACGC 4 cut(s) 112, 490, 529, 994
Csp6I GTAC 6 cut(s) 949, 1371, 1389, 1492, 1554, 1561
CspAI ACCGGT 1 cut(s) 1513
CspI CGGWCCG 2 cut(s) 1100, 1586
CviAII CATG 4 cut(s) 772, 952, 1483, 1617
CviQI GTAC 6 cut(s) 949, 1371, 1389, 1492, 1554, 1561
DdeI CTNAG 2 cut(s) 716, 1205
DinI GGCGCC 1 cut(s) 855
DpnI GATC 5 cut(s) 111, 619, 893, 1353, 1410
DpnII GATC 5 cut(s) 109, 617, 891, 1351, 1408
EaeI YGGCCR 2 cut(s) 462, 1095
EagI CGGCCG 2 cut(s) 462, 1095
Eam1104I CTCTTC 3 cut(s) 88, 560, 912
EarI CTCTTC 3 cut(s) 88, 560, 912
EciI GGCGGA 3 cut(s) 466, 1024, 1418
Ecl136II GAGCTC 2 cut(s) 275, 668
EclXI CGGCCG 2 cut(s) 462, 1095
Eco130I CCWWGG 2 cut(s) 145, 1339
Eco24I GRGCYC 4 cut(s) 277, 610, 670, 1595
Eco32I GATATC 1 cut(s) 790
Eco47I GGWCC 2 cut(s) 1100, 1586
Eco52I CGGCCG 2 cut(s) 462, 1095
Eco53kI GAGCTC 2 cut(s) 275, 668
Eco72I CACGTG 2 cut(s) 483, 1633
Eco88I CYCGRG 1 cut(s) 1297
Eco91I GGTNACC 2 cut(s) 988, 1625
EcoICRI GAGCTC 2 cut(s) 275, 668
EcoO65I GGTNACC 2 cut(s) 988, 1625
EcoRII CCWGG 2 cut(s) 1113, 1621
EcoRV GATATC 1 cut(s) 790
EcoT14I CCWWGG 2 cut(s) 145, 1339
EcoT38I GRGCYC 4 cut(s) 277, 610, 670, 1595
EgeI GGCGCC 1 cut(s) 855
EheI GGCGCC 1 cut(s) 855
ErhI CCWWGG 2 cut(s) 145, 1339
Esp3I CGTCTC 2 cut(s) 547, 753
FaeI CATG 4 cut(s) 775, 955, 1486, 1620
FatI CATG 4 cut(s) 771, 951, 1482, 1616
FauI CCCGC 1 cut(s) 898
FblI GTMKAC 2 cut(s) 1011, 1450
Fnu4HI GCNGC 8 cut(s) 420, 465, 840, 849, 852, 1135, 1266, 1653
FokI GGATG 2 cut(s) 386, 1418
FriOI GRGCYC 4 cut(s) 277, 610, 670, 1595
Fsp4HI GCNGC 8 cut(s) 420, 465, 840, 849, 852, 1135, 1266, 1653
GlaI GCGC 4 cut(s) 423, 855, 877, 1138
GluI GCNGC 8 cut(s) 420, 465, 840, 849, 852, 1135, 1266, 1653
HaeII RGCGCY 3 cut(s) 425, 857, 879
HaeIII GGCC 6 cut(s) 464, 593, 608, 1097, 1344, 1621
HgaI GACGC 4 cut(s) 112, 490, 529, 994
HhaI GCGC 4 cut(s) 424, 856, 878, 1139
Hin1I GRCGYC 4 cut(s) 104, 501, 540, 854
Hin1II CATG 4 cut(s) 775, 955, 1486, 1620
Hin6I GCGC 4 cut(s) 422, 854, 876, 1137
HinP1I GCGC 4 cut(s) 422, 854, 876, 1137
HincII GTYRAC 3 cut(s) 343, 1012, 1451
HindII GTYRAC 3 cut(s) 343, 1012, 1451
HinfI GANTC 3 cut(s) 5, 710, 909
HpaI GTTAAC 1 cut(s) 343
HphI GGTGA 9 cut(s) 550, 752, 764, 817, 977, 982, 1084, 1324, 1550
Hpy166II GTNNAC 7 cut(s) 343, 486, 706, 1012, 1075, 1451, 1480
Hpy188I TCNGA 5 cut(s) 272, 780, 981, 1351, 1356
Hpy188III TCNNGA 5 cut(s) 201, 446, 934, 1087, 1181
Hpy8I GTNNAC 7 cut(s) 343, 486, 706, 1012, 1075, 1451, 1480
HpyAV CCTTC 1 cut(s) 443
HpyCH4IV ACGT 7 cut(s) 326, 350, 440, 482, 1082, 1439, 1632
HpyCH4V TGCA 5 cut(s) 96, 123, 1486, 1568, 1652
HpyF10VI GCNNNNNNNGC 6 cut(s) 599, 845, 848, 1043, 1052, 1334
HpyF3I CTNAG 2 cut(s) 716, 1205
HpySE526I ACGT 7 cut(s) 326, 350, 440, 482, 1082, 1439, 1632
Hsp92I GRCGYC 4 cut(s) 104, 501, 540, 854
Hsp92II CATG 4 cut(s) 775, 955, 1486, 1620
HspAI GCGC 4 cut(s) 422, 854, 876, 1137
KasI GGCGCC 1 cut(s) 853
Kpn2I TCCGGA 1 cut(s) 1086
KroI GCCGGC 1 cut(s) 1399
KroNI GCCGGC 1 cut(s) 1401
KspAI GTTAAC 1 cut(s) 343
KspI CCGCGG 1 cut(s) 420
Kzo9I GATC 5 cut(s) 109, 617, 891, 1351, 1408
LmnI GCTCC 2 cut(s) 665, 1131
Lsp1109I GCAGC 2 cut(s) 851, 1664
LweI GCATC 2 cut(s) 1299, 1639
MaeII ACGT 7 cut(s) 326, 350, 440, 482, 1082, 1439, 1632
MalI GATC 5 cut(s) 111, 619, 893, 1353, 1410
MbiI CCGCTC 1 cut(s) 1134
MboI GATC 5 cut(s) 109, 617, 891, 1351, 1408
MflI RGATCY 2 cut(s) 109, 617
MhlI GDGCHC 4 cut(s) 277, 610, 670, 1595
MluCI AATT 7 cut(s) 206, 820, 1030, 1288, 1639, 1657, 1731
Mly113I GGCGCC 1 cut(s) 854
MlyI GAGTC 1 cut(s) 719
MmeI TCCRAC 2 cut(s) 998, 1502
MnlI CCTC 5 cut(s) 329, 373, 522, 1165, 1293
MroI TCCGGA 1 cut(s) 1086
MroNI GCCGGC 1 cut(s) 1399
MseI TTAA 8 cut(s) 323, 342, 677, 975, 1395, 1434, 1550, 1638
MslI CAYNNNNRTG 1 cut(s) 956
MspA1I CMGCKG 4 cut(s) 376, 419, 842, 1725
MspR9I CCNGG 5 cut(s) 595, 1115, 1590, 1610, 1623
Mva1269I GAATGC 1 cut(s) 1229
MvaI CCWGG 2 cut(s) 1115, 1623
MvnI CGCG 4 cut(s) 202, 419, 935, 1265
MwoI GCNNNNNNNGC 6 cut(s) 599, 845, 848, 1043, 1052, 1334
NaeI GCCGGC 1 cut(s) 1401
NarI GGCGCC 1 cut(s) 854
NciI CCSGG 3 cut(s) 595, 1590, 1610
NdeII GATC 5 cut(s) 109, 617, 891, 1351, 1408
NgoMIV GCCGGC 1 cut(s) 1399
NlaIII CATG 4 cut(s) 775, 955, 1486, 1620
NlaIV GGNNCC 4 cut(s) 111, 573, 608, 855
NmuCI GTSAC 7 cut(s) 711, 740, 898, 965, 988, 1048, 1666
NruI TCGCGA 2 cut(s) 202, 935
NspI RCATGY 1 cut(s) 1486
NspV TTCGAA 1 cut(s) 333
PctI GAATGC 1 cut(s) 1229
PdiI GCCGGC 1 cut(s) 1401
PfeI GAWTC 2 cut(s) 5, 909
PflMI CCANNNNNTGG 1 cut(s) 567
PinAI ACCGGT 1 cut(s) 1513
PkrI GCNGC 8 cut(s) 421, 466, 841, 850, 853, 1136, 1267, 1654
PleI GAGTC 1 cut(s) 718
PluTI GGCGCC 1 cut(s) 857
PmaCI CACGTG 2 cut(s) 483, 1633
PmlI CACGTG 2 cut(s) 483, 1633
PpsI GAGTC 1 cut(s) 718
Ppu21I YACGTR 2 cut(s) 483, 1633
PshAI GACNNNNGTC 1 cut(s) 986
PshBI ATTAAT 2 cut(s) 975, 1638
Psp124BI GAGCTC 2 cut(s) 277, 670
Psp1406I AACGTT 1 cut(s) 1082
Psp6I CCWGG 2 cut(s) 1113, 1621
PspCI CACGTG 2 cut(s) 483, 1633
PspEI GGTNACC 2 cut(s) 988, 1625
PspGI CCWGG 2 cut(s) 1113, 1621
PspN4I GGNNCC 4 cut(s) 111, 573, 608, 855
PspOMI GGGCCC 1 cut(s) 606
PspPI GGNCC 5 cut(s) 591, 606, 607, 1100, 1586
PsrI GAACNNNNNNTAC 2 cut(s) 932, 964
PsuI RGATCY 2 cut(s) 109, 617
PvuII CAGCTG 1 cut(s) 842
RruI TCGCGA 2 cut(s) 202, 935
RsaI GTAC 6 cut(s) 950, 1372, 1390, 1493, 1555, 1562
RsaNI GTAC 6 cut(s) 949, 1371, 1389, 1492, 1554, 1561
RseI CAYNNNNRTG 1 cut(s) 956
Rsr2I CGGWCCG 2 cut(s) 1100, 1586
RsrII CGGWCCG 2 cut(s) 1100, 1586
SacI GAGCTC 2 cut(s) 277, 670
SacII CCGCGG 1 cut(s) 420
SalI GTCGAC 2 cut(s) 1010, 1449
SaqAI TTAA 8 cut(s) 323, 342, 677, 975, 1395, 1434, 1550, 1638
SatI GCNGC 8 cut(s) 420, 465, 840, 849, 852, 1135, 1266, 1653
Sau3AI GATC 5 cut(s) 109, 617, 891, 1351, 1408
Sau96I GGNCC 5 cut(s) 591, 606, 607, 1100, 1586
SchI GAGTC 1 cut(s) 719
ScrFI CCNGG 5 cut(s) 595, 1115, 1590, 1610, 1623
SduI GDGCHC 4 cut(s) 277, 610, 670, 1595
SfaNI GCATC 2 cut(s) 1299, 1639
SfcI CTRYAG 2 cut(s) 843, 1156
SfoI GGCGCC 1 cut(s) 855
Sfr303I CCGCGG 1 cut(s) 420
SfuI TTCGAA 1 cut(s) 333
SgrBI CCGCGG 1 cut(s) 420
SgrDI CGTCGACG 1 cut(s) 1010
SinI GGWCC 2 cut(s) 1100, 1586
SmiMI CAYNNNNRTG 1 cut(s) 956
Sse9I AATT 7 cut(s) 206, 820, 1030, 1288, 1639, 1657, 1731
SspDI GGCGCC 1 cut(s) 853
SspI AATATT 1 cut(s) 15
SstI GAGCTC 2 cut(s) 277, 670
StyD4I CCNGG 5 cut(s) 593, 1113, 1588, 1608, 1621
StyI CCWWGG 2 cut(s) 145, 1339
TaiI ACGT 7 cut(s) 329, 353, 443, 485, 1085, 1442, 1635
TaqI TCGA 9 cut(s) 222, 333, 629, 1011, 1170, 1245, 1418, 1450, 1683
TasI AATT 7 cut(s) 206, 820, 1030, 1288, 1639, 1657, 1731
TatI WGTACW 2 cut(s) 1553, 1560
TauI GCSGC 6 cut(s) 422, 467, 851, 854, 1137, 1268
TfiI GAWTC 2 cut(s) 5, 909
Tru1I TTAA 8 cut(s) 323, 342, 677, 975, 1395, 1434, 1550, 1638
Tru9I TTAA 8 cut(s) 323, 342, 677, 975, 1395, 1434, 1550, 1638
TscAI CASTG 3 cut(s) 1372, 1651, 1675
TseFI GTSAC 7 cut(s) 711, 740, 898, 965, 988, 1048, 1666
TseI GCWGC 2 cut(s) 839, 1652
Tsp45I GTSAC 7 cut(s) 711, 740, 898, 965, 988, 1048, 1666
TspDTI ATGAA 3 cut(s) 7, 615, 1725
TspGWI ACGGA 8 cut(s) 118, 638, 647, 678, 780, 825, 885, 1144
TspRI CASTG 3 cut(s) 1372, 1651, 1675
Van91I CCANNNNNTGG 1 cut(s) 567
VpaK11BI GGWCC 2 cut(s) 1100, 1586
VspI ATTAAT 2 cut(s) 975, 1638
XapI RAATTY 2 cut(s) 206, 820
XceI RCATGY 1 cut(s) 1486
XmiI GTMKAC 2 cut(s) 1011, 1450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.