MD03G1291000.v1.1

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000522
Physical Location & Seq
Reverse (-)
36838382 .. 36840289
1908 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1291000.v1.1.491

Sequence Viewer

Length: 1605 bp
ATGGCTAATTTGCAACTATTCTCCATGTCCAAAATTTCGGTTCTCATCCTTCTTCTTTCGACGTGTTTTGCATCTCCATCTTTAGCTGCAGATGCAAATCCAGTCTCCAAGCAAACCATTTGCAAATTCACCCCAAACCCTACATACTGCAACTCCGTCCTCCCCAACCAGACCTCAAATGCCTACGACTTTGGCCGCTATGCTGCGCAAAAGTCGTTATCACAGAGTCTCAAATTCCTTAACTTGGTGGACAAGTATCTCCGAAGCCACTCTGCCACTCTCTCACAAAACTCGGTCCTCGCTCTCCAGGATTGCCAGTTTCTTGCAGGGCTCAATGTAGATTTTCTTGCAAGCTCCCTGGAAACCCTAAACAACACCAATTATCCAACACTTTCTGACTCGAAAACCAACGACATCCAGACCATGCTCAGCGCCATCTTAACAAACCAGCAAACATGTTTGGATGGCATACAAGCCACAGCTTCATCTTGGAGTGTAAAAAGTGGTCTTTCGGTTCCACTCTCTAACGACACCAAACTGTACAGTGTTTCTCTGGCCTTGTTCAACAAGGCATGGGTGCCTAAGAACAAGAAGAAGAAGGGGAGAAAACTGCTTGATAATCAGATCAACGTTGATGACGTGTTGGTCAAGGAAATAGTGACTGTGAGTCAGGACGGAAGTGGGAACTTCACCACCATAAACGATGCCGTTGCAGCCGCTCCAAACAAGTCTGAAGCCTCTAAAGGTTACTTCTTGATTTACATCAAGGCAGGAGTTTATGAAGAGTATGTGACCATTGACAAGAAAAAGAAGTACCTCATGATGGTTGGCGATGGCATCAATCAGACTGTCCTCACTGGCGACCGAAATGTTGCAGATGGAAACTGGACTACTTTCAAATCAGCAACATTTGCTGTAGTTGGACAAGGGTTTGTGGCAGTCAACATGACGTTTCGTAACACCGCCGGGCCCTGGAAGCACCAAGCAGTCGCGGTTCGAAACGGGGCTGATCTATCCACATTCTATAGGTGCAGCATTGAAGGGTACCAAGACACCTTATACACACATTCTATGAGGCAATTCTACAGAGAATGTGACATCTATGGCACCGTAGACTTCATATTTGGTAATGCTGCAGTTGTCTTCCAAAACTGCAACATTTATCCCCGCCTGCCGATGAGCGGCCAATTCAATGCGGTCACGGCTCAAGGTCGAACTGACCCGAACCAAAACACGGGCACTTTGATTCACAATTGTACCATACGAGCTGCCGATGATCTGGCTTTGAGCGACGGTGGTACAAAGACTTATCTCGGAAGGCCATGGAAAGAGTATTCTAGGACAATTTACATGCAATCCTTCATGGATGGTTTGATTGATCAAGACGGTTGGCATGAATGGGATGGAGATTTTGCCCTAAGCACTTTGTATTATGCGGAGTACGATAGCAGTGGACCCGGTTCGGACACTAAAAGCCGAGTTGCATGGGCGGGCTATCATGTGATTGATGCTAGTGATGCTTCAAATTTTACAGTATCCAATTTCTTGCTGGGAGATGCTTGGCTGCCTCAAACTGGAGTGCCTTTTTCTGGTGGCTTATACTAA

Protein Analysis

535

Amino Acids

58.68

Weight (kDa)

6.32

Isoelectric Point (pI)

27.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 38 - 187 6.4e-25 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 220 - 519 2.4e-139 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000522)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02810 AT2G47550 AT4G02330
fragaria_vesca FvH4_2g25960 FvH4_2g25960 FvH4_2g25970 FvH4_5g35570 FvH4_5g35580 FvH4_5g35590
malus_domestica MD03G1290900.v1.1 MD03G1291000.v1.1 MD08G1195600.v1.1 MD11G1307400.v1.1 MD11G1307500.v1.1
prunus_persica Prupe.1G529400_v2.0.a1 Prupe.1G529500_v2.0.a1 Prupe.8G263900_v2.0.a1 Prupe.8G264000_v2.0.a1
pyrus_communis pycom08g16800
rosa_chinensis RchiOBHm_Chr6g0294321 RchiOBHm_Chr6g0294331 RchiOBHm_Chr7g0236951 RchiOBHm_Chr7g0236971 RchiOBHm_Chr7g0237041 RchiOBHm_Chr7g0237051
rosa_laevigata RLG00000001034 RLG00000001035 RLG00000001039 RLG00000001043 RLG00000001045 RLG00000011880 RLG00000011881 RLG00000034181
rosa_multiflora Rmu_co8086188.1_g000001 Rmu_co8458485.1_g000001 Rmu_sc0000272.1_g000007 Rmu_sc0000272.1_g000009 Rmu_sc0002131.1_g000030 Rmu_sc0002131.1_g000037 Rmu_sc0005369.1_g000005 Rmu_sc0005369.1_g000008 Rmu_sc0007991.1_g000005 Rmu_sc0007991.1_g000013 Rmu_sc0033563.1_g000001
rosa_roxburghii Rroxscaffold_3G00224540 Rroxscaffold_3G00224600 Rroxscaffold_3G00224650 Rroxscaffold_3G00224660 Rroxscaffold_7G00173470 Rroxscaffold_7G00173480
rosa_rugosa Rorug06G0243400 Rorug06G0243500 Rorug07G0301200 Rorug07G0301500 Rorug07G0301600
rosa_samantha Rh6AG356000 Rh6AG356100 Rh6BG362600 Rh6BG362800 Rh6CG370000 Rh6CG370100 Rh6DG356500 Rh6DG356600 Rh7AG456200 Rh7AG456500 Rh7AG457500 Rh7AG457600 Rh7BG427500 Rh7BG427700 Rh7BG428400 Rh7BG428500 Rh7CG475100 Rh7CG475300 Rh7CG475700 Rh7CG475800 Rh7DG444100 Rh7DG444300 Rh7DG444700 Rh7DG444900
rosa_wichuraiana Rw6G031020 Rw6G031030 Rw7G037870 Rw7G037880 Rw7G037920 Rw7G037940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 644
Acc16I TGCGCA 1 cut(s) 207
Acc65I GGTACC 1 cut(s) 1044
AccB1I GGYRCC 3 cut(s) 577, 1044, 1106
AccBSI CCGCTC 2 cut(s) 719, 1182
AccI GTMKAC 1 cut(s) 1113
AccII CGCG 1 cut(s) 992
AciI CCGC 9 cut(s) 196, 717, 963, 992, 1168, 1182, 1196, 1436, 1490
AclI AACGTT 1 cut(s) 630
AcoI YGGCCR 2 cut(s) 193, 1183
AcsI RAATTY 4 cut(s) 33, 125, 233, 1525
AcuI CTGAAG 1 cut(s) 753
AfaI GTAC 6 cut(s) 542, 815, 1046, 1258, 1300, 1442
AfiI CCNNNNNNNGG 7 cut(s) 244, 823, 972, 1181, 1234, 1574, 1589
AflIII ACRYGT 3 cut(s) 62, 455, 639
AgsI TTSAA 5 cut(s) 565, 898, 1040, 1192, 1524
AhdI GACNNNNNGTC 1 cut(s) 666
AjiI CACGTC 2 cut(s) 63, 640
AjnI CCWGG 3 cut(s) 306, 357, 971
AluBI AGCT 4 cut(s) 86, 354, 482, 1268
AluI AGCT 4 cut(s) 86, 354, 482, 1268
Alw26I GTCTC 2 cut(s) 109, 233
AoxI GGCC 5 cut(s) 193, 555, 968, 1183, 1319
ApaI GGGCCC 1 cut(s) 972
ApeKI GCWGC 7 cut(s) 86, 203, 713, 1032, 1133, 1268, 1564
ApoI RAATTY 4 cut(s) 33, 125, 233, 1525
ArsI GACNNNNNNTTYG 2 cut(s) 1106, 1138
Asp718I GGTACC 1 cut(s) 1044
AspLEI GCGC 2 cut(s) 208, 434
AspS9I GGNCC 4 cut(s) 295, 968, 969, 1454
AsuC2I CCSGG 2 cut(s) 967, 1458
AsuHPI GGTGA 2 cut(s) 121, 682
AsuII TTCGAA 1 cut(s) 997
AvaII GGWCC 2 cut(s) 295, 1454
BaeGI GKGCMC 2 cut(s) 972, 1241
BaeI ACNNNNGTAYC 2 cut(s) 1240, 1273
BanI GGYRCC 3 cut(s) 577, 1044, 1106
BanII GRGCYC 2 cut(s) 333, 972
BbsI GAAGAC 1 cut(s) 1135
BbvI GCAGC 7 cut(s) 73, 190, 725, 1044, 1120, 1255, 1551
BccI CCATC 8 cut(s) 85, 443, 458, 817, 827, 872, 1361, 1397
BceAI ACGGC 2 cut(s) 692, 1218
BcgI CGANNNNNNTGC 2 cut(s) 692, 726
BciT130I CCWGG 3 cut(s) 308, 359, 973
BciVI GTATCC 1 cut(s) 1546
BclI TGATCA 1 cut(s) 1378
BcnI CCSGG 2 cut(s) 967, 1458
BcoDI GTCTC 2 cut(s) 109, 233
BfaI CTAG 2 cut(s) 1338, 1512
BfmI CTRYAG 5 cut(s) 87, 915, 1024, 1084, 1134
BfoI RGCGCY 1 cut(s) 435
BfuI GTATCC 1 cut(s) 1546
BlpI GCTNAGC 1 cut(s) 428
Bme1390I CCNGG 5 cut(s) 308, 359, 967, 973, 1458
Bme18I GGWCC 2 cut(s) 295, 1454
BmeRI GACNNNNNGTC 1 cut(s) 666
BmgBI CACGTC 2 cut(s) 63, 640
BmgT120I GGNCC 4 cut(s) 295, 968, 969, 1454
BmiI GGNNCC 6 cut(s) 516, 579, 970, 1046, 1108, 1456
BmrFI CCNGG 5 cut(s) 308, 359, 967, 973, 1458
BmsI GCATC 7 cut(s) 80, 82, 694, 846, 1498, 1507, 1546
BpiI GAAGAC 1 cut(s) 1135
BpmI CTGGAG 2 cut(s) 290, 1596
Bpu10I CCTNAGC 1 cut(s) 1418
Bpu1102I GCTNAGC 1 cut(s) 428
Bpu14I TTCGAA 1 cut(s) 997
BpuEI CTTGAG 1 cut(s) 1191
BpuMI CCSGG 2 cut(s) 967, 1458
BsaBI GATNNNNATC 2 cut(s) 96, 761
BsaJI CCNNGG 3 cut(s) 357, 971, 1322
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bsc4I CCNNNNNNNGG 7 cut(s) 244, 823, 972, 1181, 1234, 1574, 1589
Bse1I ACTGG 5 cut(s) 101, 316, 862, 890, 1579
Bse8I GATNNNNATC 2 cut(s) 96, 761
BseBI CCWGG 3 cut(s) 308, 359, 973
BseDI CCNNGG 3 cut(s) 357, 971, 1322
BseGI GGATG 5 cut(s) 45, 414, 469, 1372, 1408
BseJI GATNNNNATC 2 cut(s) 96, 761
BseLI CCNNNNNNNGG 7 cut(s) 244, 823, 972, 1181, 1234, 1574, 1589
BseMII CTCAG 1 cut(s) 442
BseNI ACTGG 5 cut(s) 101, 316, 862, 890, 1579
BseSI GKGCMC 2 cut(s) 972, 1241
BseXI GCAGC 7 cut(s) 73, 190, 725, 1044, 1120, 1255, 1551
BseYI CCCAGC 1 cut(s) 1549
BsgI GTGCAG 1 cut(s) 1051
Bsh1236I CGCG 1 cut(s) 992
Bsh1285I CGRYCG 1 cut(s) 865
BshFI GGCC 5 cut(s) 195, 557, 970, 1185, 1321
BshNI GGYRCC 3 cut(s) 577, 1044, 1106
BsiEI CGRYCG 1 cut(s) 865
BsiSI CCGG 2 cut(s) 966, 1458
BslI CCNNNNNNNGG 7 cut(s) 244, 823, 972, 1181, 1234, 1574, 1589
BsmAI GTCTC 2 cut(s) 109, 233
BsnI GGCC 5 cut(s) 195, 557, 970, 1185, 1321
Bsp119I TTCGAA 1 cut(s) 997
Bsp120I GGGCCC 1 cut(s) 968
Bsp1286I GDGCHC 3 cut(s) 333, 972, 1241
Bsp1407I TGTACA 1 cut(s) 540
Bsp143I GATC 4 cut(s) 624, 1009, 1276, 1378
Bsp1720I GCTNAGC 1 cut(s) 428
Bsp19I CCATGG 1 cut(s) 1322
BspACI CCGC 9 cut(s) 196, 717, 963, 992, 1168, 1182, 1196, 1436, 1490
BspANI GGCC 5 cut(s) 195, 557, 970, 1185, 1321
BspCNI CTCAG 1 cut(s) 441
BspFNI CGCG 1 cut(s) 992
BspHI TCATGA 1 cut(s) 819
BspLI GGNNCC 6 cut(s) 516, 579, 970, 1046, 1108, 1456
BspMAI CTGCAG 2 cut(s) 91, 1138
BspT104I TTCGAA 1 cut(s) 997
BspT107I GGYRCC 3 cut(s) 577, 1044, 1106
BsrBI CCGCTC 2 cut(s) 719, 1182
BsrGI TGTACA 1 cut(s) 540
BsrI ACTGG 5 cut(s) 101, 316, 862, 890, 1579
BssECI CCNNGG 3 cut(s) 357, 971, 1322
BssMI GATC 4 cut(s) 624, 1009, 1276, 1378
BssT1I CCWWGG 1 cut(s) 1322
Bst2UI CCWGG 3 cut(s) 308, 359, 973
Bst4CI ACNGT 8 cut(s) 540, 545, 664, 850, 1111, 1295, 1388, 1534
Bst6I CTCTTC 1 cut(s) 777
BstAPI GCANNNNNTGC 1 cut(s) 911
BstAUI TGTACA 1 cut(s) 540
BstBI TTCGAA 1 cut(s) 997
BstC8I GCNNGC 3 cut(s) 352, 1172, 1492
BstDEI CTNAG 3 cut(s) 428, 582, 1418
BstDSI CCRYGG 1 cut(s) 1322
BstF5I GGATG 5 cut(s) 45, 414, 469, 1372, 1408
BstFNI CGCG 1 cut(s) 992
BstH2I RGCGCY 1 cut(s) 435
BstHHI GCGC 2 cut(s) 208, 434
BstKTI GATC 4 cut(s) 627, 1012, 1279, 1381
BstMAI GTCTC 2 cut(s) 109, 233
BstMBI GATC 4 cut(s) 624, 1009, 1276, 1378
BstMCI CGRYCG 1 cut(s) 865
BstMWI GCNNNNNNNGC 6 cut(s) 92, 713, 911, 976, 1202, 1517
BstNI CCWGG 3 cut(s) 308, 359, 973
BstNSI RCATGY 2 cut(s) 459, 1354
BstSCI CCNGG 5 cut(s) 306, 357, 965, 971, 1456
BstSFI CTRYAG 5 cut(s) 87, 915, 1024, 1084, 1134
BstSLI GKGCMC 2 cut(s) 972, 1241
BstUI CGCG 1 cut(s) 992
BstV1I GCAGC 7 cut(s) 73, 190, 725, 1044, 1120, 1255, 1551
BstV2I GAAGAC 1 cut(s) 1135
BsuI GTATCC 1 cut(s) 1546
BsuRI GGCC 5 cut(s) 195, 557, 970, 1185, 1321
BtgI CCRYGG 1 cut(s) 1322
BtgZI GCGATG 1 cut(s) 846
BtrI CACGTC 2 cut(s) 63, 640
BtsCI GGATG 5 cut(s) 45, 414, 469, 1372, 1408
BtsI GCAGTG 1 cut(s) 1456
BtsIMutI CAGTG 3 cut(s) 550, 855, 1456
Cac8I GCNNGC 3 cut(s) 352, 1172, 1492
CciI TCATGA 1 cut(s) 819
CfoI GCGC 2 cut(s) 208, 434
Cfr13I GGNCC 4 cut(s) 295, 968, 969, 1454
Csp6I GTAC 6 cut(s) 541, 814, 1045, 1257, 1299, 1441
CviQI GTAC 6 cut(s) 541, 814, 1045, 1257, 1299, 1441
DdeI CTNAG 3 cut(s) 428, 582, 1418
DpnI GATC 4 cut(s) 626, 1011, 1278, 1380
DpnII GATC 4 cut(s) 624, 1009, 1276, 1378
DrdI GACNNNNNNGTC 1 cut(s) 644
DriI GACNNNNNGTC 1 cut(s) 666
DseDI GACNNNNNNGTC 1 cut(s) 644
EaeI YGGCCR 2 cut(s) 193, 1183
Eam1104I CTCTTC 1 cut(s) 777
Eam1105I GACNNNNNGTC 1 cut(s) 666
EarI CTCTTC 1 cut(s) 777
Eco130I CCWWGG 1 cut(s) 1322
Eco24I GRGCYC 2 cut(s) 333, 972
Eco47I GGWCC 2 cut(s) 295, 1454
Eco57I CTGAAG 1 cut(s) 753
EcoO109I RGGNCCY 1 cut(s) 969
EcoRII CCWGG 3 cut(s) 306, 357, 971
EcoT14I CCWWGG 1 cut(s) 1322
EcoT38I GRGCYC 2 cut(s) 333, 972
ErhI CCWWGG 1 cut(s) 1322
FauI CCCGC 2 cut(s) 1175, 1483
FbaI TGATCA 1 cut(s) 1378
FblI GTMKAC 1 cut(s) 1113
FokI GGATG 5 cut(s) 32, 401, 476, 1379, 1415
FriOI GRGCYC 2 cut(s) 333, 972
FspBI CTAG 2 cut(s) 1338, 1512
FspI TGCGCA 1 cut(s) 207
GlaI GCGC 2 cut(s) 207, 433
GsaI CCCAGC 1 cut(s) 1553
GsuI CTGGAG 2 cut(s) 290, 1596
HaeII RGCGCY 1 cut(s) 435
HaeIII GGCC 5 cut(s) 195, 557, 970, 1185, 1321
HapII CCGG 2 cut(s) 966, 1458
HhaI GCGC 2 cut(s) 208, 434
Hin6I GCGC 2 cut(s) 206, 432
HinP1I GCGC 2 cut(s) 206, 432
HincII GTYRAC 1 cut(s) 943
HindII GTYRAC 1 cut(s) 943
HinfI GANTC 4 cut(s) 226, 398, 667, 1246
HpaII CCGG 2 cut(s) 966, 1458
HphI GGTGA 2 cut(s) 121, 682
Hpy166II GTNNAC 4 cut(s) 250, 943, 1114, 1454
Hpy188I TCNGA 7 cut(s) 263, 397, 624, 733, 846, 1316, 1465
Hpy188III TCNNGA 5 cut(s) 418, 671, 754, 820, 1382
Hpy8I GTNNAC 4 cut(s) 250, 943, 1114, 1454
Hpy99I CGWCG 2 cut(s) 64, 1295
HpyAV CCTTC 5 cut(s) 59, 592, 1034, 1311, 1369
HpyCH4III ACNGT 8 cut(s) 540, 545, 664, 850, 1111, 1295, 1388, 1534
HpyCH4IV ACGT 4 cut(s) 62, 630, 639, 950
HpyF10VI GCNNNNNNNGC 6 cut(s) 92, 713, 911, 976, 1202, 1517
HpyF3I CTNAG 3 cut(s) 428, 582, 1418
HpySE526I ACGT 4 cut(s) 62, 630, 639, 950
HspAI GCGC 2 cut(s) 206, 432
KpnI GGTACC 1 cut(s) 1048
Ksp22I TGATCA 1 cut(s) 1378
Kzo9I GATC 4 cut(s) 624, 1009, 1276, 1378
LmnI GCTCC 2 cut(s) 359, 724
Lsp1109I GCAGC 7 cut(s) 73, 190, 725, 1044, 1120, 1255, 1551
LweI GCATC 7 cut(s) 80, 82, 694, 846, 1498, 1507, 1546
MaeI CTAG 2 cut(s) 1338, 1512
MaeII ACGT 4 cut(s) 62, 630, 639, 950
MaeIII GTNAC 6 cut(s) 658, 746, 790, 956, 1094, 1198
MalI GATC 4 cut(s) 626, 1011, 1278, 1380
MbiI CCGCTC 2 cut(s) 719, 1182
MboI GATC 4 cut(s) 624, 1009, 1276, 1378
MboII GAAGA 5 cut(s) 44, 604, 607, 794, 1135
MfeI CAATTG 1 cut(s) 1252
MhlI GDGCHC 3 cut(s) 333, 972, 1241
MlyI GAGTC 3 cut(s) 235, 392, 676
MmeI TCCRAC 2 cut(s) 410, 901
MnlI CCTC 8 cut(s) 170, 184, 308, 748, 827, 863, 1068, 1578
MseI TTAA 2 cut(s) 240, 440
MspI CCGG 2 cut(s) 966, 1458
MspR9I CCNGG 5 cut(s) 308, 359, 967, 973, 1458
MunI CAATTG 1 cut(s) 1252
MvaI CCWGG 3 cut(s) 308, 359, 973
MvnI CGCG 1 cut(s) 992
MwoI GCNNNNNNNGC 6 cut(s) 92, 713, 911, 976, 1202, 1517
NciI CCSGG 2 cut(s) 967, 1458
NcoI CCATGG 1 cut(s) 1322
NdeII GATC 4 cut(s) 624, 1009, 1276, 1378
NlaIV GGNNCC 6 cut(s) 516, 579, 970, 1046, 1108, 1456
NmeAIII GCCGAG 1 cut(s) 1502
NmuCI GTSAC 4 cut(s) 658, 790, 1094, 1198
NsbI TGCGCA 1 cut(s) 207
NspI RCATGY 2 cut(s) 459, 1354
NspV TTCGAA 1 cut(s) 997
PagI TCATGA 1 cut(s) 819
PciI ACATGT 1 cut(s) 455
PcsI WCGNNNNNNNCGW 1 cut(s) 636
PfeI GAWTC 1 cut(s) 1246
PfoI TCCNGGA 1 cut(s) 306
PleI GAGTC 3 cut(s) 234, 392, 675
PpsI GAGTC 3 cut(s) 234, 392, 675
PscI ACATGT 1 cut(s) 455
Psp1406I AACGTT 1 cut(s) 630
Psp6I CCWGG 3 cut(s) 306, 357, 971
PspFI CCCAGC 1 cut(s) 1549
PspGI CCWGG 3 cut(s) 306, 357, 971
PspN4I GGNNCC 6 cut(s) 516, 579, 970, 1046, 1108, 1456
PspOMI GGGCCC 1 cut(s) 968
PspPI GGNCC 4 cut(s) 295, 968, 969, 1454
PstI CTGCAG 2 cut(s) 91, 1138
RsaI GTAC 6 cut(s) 542, 815, 1046, 1258, 1300, 1442
RsaNI GTAC 6 cut(s) 541, 814, 1045, 1257, 1299, 1441
SaqAI TTAA 2 cut(s) 240, 440
Sau3AI GATC 4 cut(s) 624, 1009, 1276, 1378
Sau96I GGNCC 4 cut(s) 295, 968, 969, 1454
SchI GAGTC 3 cut(s) 235, 392, 676
ScrFI CCNGG 5 cut(s) 308, 359, 967, 973, 1458
SduI GDGCHC 3 cut(s) 333, 972, 1241
SfaNI GCATC 7 cut(s) 80, 82, 694, 846, 1498, 1507, 1546
SfcI CTRYAG 5 cut(s) 87, 915, 1024, 1084, 1134
SfuI TTCGAA 1 cut(s) 997
SinI GGWCC 2 cut(s) 295, 1454
SmlI CTYRAG 1 cut(s) 1206
SmoI CTYRAG 1 cut(s) 1206
SsiI CCGC 9 cut(s) 196, 717, 963, 992, 1168, 1182, 1196, 1436, 1490
SspMI CTAG 2 cut(s) 1338, 1512
StyD4I CCNGG 5 cut(s) 306, 357, 965, 971, 1456
StyI CCWWGG 1 cut(s) 1322
TaaI ACNGT 8 cut(s) 540, 545, 664, 850, 1111, 1295, 1388, 1534
TaiI ACGT 4 cut(s) 65, 633, 642, 953
TaqI TCGA 4 cut(s) 59, 401, 997, 1213
TaqII GACCGA 2 cut(s) 283, 879
TatI WGTACW 1 cut(s) 540
TauI GCSGC 3 cut(s) 198, 719, 1185
TfiI GAWTC 1 cut(s) 1246
Tru1I TTAA 2 cut(s) 240, 440
Tru9I TTAA 2 cut(s) 240, 440
TscAI CASTG 3 cut(s) 550, 862, 1456
TseFI GTSAC 4 cut(s) 658, 790, 1094, 1198
TseI GCWGC 7 cut(s) 86, 203, 713, 1032, 1133, 1268, 1564
Tsp45I GTSAC 4 cut(s) 658, 790, 1094, 1198
TspDTI ATGAA 5 cut(s) 474, 795, 1108, 1351, 1410
TspGWI ACGGA 2 cut(s) 145, 690
TspRI CASTG 3 cut(s) 550, 862, 1456
VpaK11BI GGWCC 2 cut(s) 295, 1454
XapI RAATTY 4 cut(s) 33, 125, 233, 1525
XceI RCATGY 2 cut(s) 459, 1354
XcmI CCANNNNNNNNNTGG 1 cut(s) 1546
XmiI GTMKAC 1 cut(s) 1113
XspI CTAG 2 cut(s) 1338, 1512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.