Rh7CG475800

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0000522
Physical Location & Seq
Forward (+)
63881531 .. 63883255
1725 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG475800.1

Sequence Viewer

Length: 1413 bp
ATGATCTCCCTCAACCAATACCTCCAAAGTTACCGTTCTGCTTTATCGCAAACCGCCATTCAAGCCCTCGAGGATTGCAAGTTCCTCGCGTCCCTGAACATCGACTTCATCTCAAACACCATAAAAAAAGTTAACAGCAGCAGCGACGCCGTGCTTCCGAGCTCGGCCGCAGAAGATCTCCAGACATTGCTCAGTGCCATCATGACAAACCAGCAGACTTGTTCCGACGCACTCCAATCTGTCACTACTGTCAAAGACGAGCTCTTAGTCCCGCTCTTAAACTGTACAAGTATGTACAGCGTCTCTTTGGCTCTGTTCACAGAGGGTTGGGTGCCAAAGGATCACAAAAATGCTAACGGGAAGCCGAACCATCTTAGTTTTCGAAAGGGTAGGTTGTCACTGAAAATGTCGAGCAAAGCCCGTGGGGTCTACGACTGGGTGATAAGTAGGCGAGGAAGGCGGCTTCTTCAGGCGGCTGGAGATGAGGAAGTTGTGGTTAGGGACATTGTGGTGGTGAGCCAGGATGGAACGCCGAACAACTCTGTTGCTAGTGATGGGTATTTCTTGGTGTATGTCACCGCCGGTGTTTATGAAGAGTATGTTTCGATTGCGTCGAACAAGCGGTACTTGTTGCTGATCGGAGACGGTATCAATCAGACCATCATCACCGGGAACCGCAATGTCGTCGATGGATCGACTACTTTCGACTCTGCTACTTTAGCTGTGGTGGCAGAAGGGTTTGTGGGAGTCAACATAACCATTCGTAACACGGCTGGACCAAGTAAAATGCAGGCTGTGGCGGTCAGAAACGGCGCGGACTTGTCAGTCTTCTACAGCTGCAGCTTTGAAGCCTACCAGGACACCCTGTACGCGCATTCTCTCCGACAATTCTACCGAGAATGTGACATCTATGGAACTGTAGATTTCATATTCGGCAATGCTGCAGTTGTTTTACAAAACTGTAACCTCTACCCTCGCCAGCCACTCAAAGGCCAATCCAATCCCATCACGGCTCAGGGCCGAACTGACCCGAATCAGAATACGGGCACTTCCATCCAGAACTGCACCATTACAGCCGCTGCGGACTTGGCTTCGAGTAACTTCACTGTCAAGACATATCTAGGGAGGCCATGGAAGGAGTATTCGAGGACGGTTTACTTGCAGAATTTCATGGATAGTTTGATTGATCCTGTTGGTTGGCTCGAGTGGAATGGAGATTTTGCACTGAGCACATTGTATTATGCTGAGTATGATAACAGAGGACCTGGATCAGACACTAGTAACAGAGTCACATGGCCTGGTTACCATGTGATCAATGCAACTGATGCTGTTAATTTTACAGTGTCTAACTTTCTGGTAGGGGATAGCTGGTTGCCTCAGACAGGTGTGCCTTATACTGGTGGCTTTGTTTGA

Protein Analysis

470

Amino Acids

51.57

Weight (kDa)

5.37

Isoelectric Point (pI)

35.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 16 - 105 4.6e-14 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 184 - 455 2.1e-129 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000522)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02810 AT2G47550 AT4G02330
fragaria_vesca FvH4_2g25960 FvH4_2g25960 FvH4_2g25970 FvH4_5g35570 FvH4_5g35580 FvH4_5g35590
malus_domestica MD03G1290900.v1.1 MD03G1291000.v1.1 MD08G1195600.v1.1 MD11G1307400.v1.1 MD11G1307500.v1.1
prunus_persica Prupe.1G529400_v2.0.a1 Prupe.1G529500_v2.0.a1 Prupe.8G263900_v2.0.a1 Prupe.8G264000_v2.0.a1
pyrus_communis pycom08g16800
rosa_chinensis RchiOBHm_Chr6g0294321 RchiOBHm_Chr6g0294331 RchiOBHm_Chr7g0236951 RchiOBHm_Chr7g0236971 RchiOBHm_Chr7g0237041 RchiOBHm_Chr7g0237051
rosa_laevigata RLG00000001034 RLG00000001035 RLG00000001039 RLG00000001043 RLG00000001045 RLG00000011880 RLG00000011881 RLG00000034181
rosa_multiflora Rmu_co8086188.1_g000001 Rmu_co8458485.1_g000001 Rmu_sc0000272.1_g000007 Rmu_sc0000272.1_g000009 Rmu_sc0002131.1_g000030 Rmu_sc0002131.1_g000037 Rmu_sc0005369.1_g000005 Rmu_sc0005369.1_g000008 Rmu_sc0007991.1_g000005 Rmu_sc0007991.1_g000013 Rmu_sc0033563.1_g000001
rosa_roxburghii Rroxscaffold_3G00224540 Rroxscaffold_3G00224600 Rroxscaffold_3G00224650 Rroxscaffold_3G00224660 Rroxscaffold_7G00173470 Rroxscaffold_7G00173480
rosa_rugosa Rorug06G0243400 Rorug06G0243500 Rorug07G0301200 Rorug07G0301500 Rorug07G0301600
rosa_samantha Rh6AG356000 Rh6AG356100 Rh6BG362600 Rh6BG362800 Rh6CG370000 Rh6CG370100 Rh6DG356500 Rh6DG356600 Rh7AG456200 Rh7AG456500 Rh7AG457500 Rh7AG457600 Rh7BG427500 Rh7BG427700 Rh7BG428400 Rh7BG428500 Rh7CG475100 Rh7CG475300 Rh7CG475700 Rh7CG475800 Rh7DG444100 Rh7DG444300 Rh7DG444700 Rh7DG444900
rosa_wichuraiana Rw6G031020 Rw6G031030 Rw7G037870 Rw7G037880 Rw7G037920 Rw7G037940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 824
AbsI CCTCGAGG 1 cut(s) 68
AccB1I GGYRCC 1 cut(s) 331
AccBSI CCGCTC 1 cut(s) 274
AccI GTMKAC 1 cut(s) 429
AccII CGCG 3 cut(s) 89, 815, 872
AclWI GGATC 4 cut(s) 348, 700, 1181, 1276
AcoI YGGCCR 1 cut(s) 165
AcsI RAATTY 1 cut(s) 1165
AcuI CTGAAG 1 cut(s) 452
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 4 cut(s) 286, 296, 626, 869
AfiI CCNNNNNNNGG 3 cut(s) 989, 1382, 1397
AgsI TTSAA 2 cut(s) 62, 848
AhlI ACTAGT 1 cut(s) 1277
AjnI CCWGG 4 cut(s) 519, 855, 1264, 1297
AloI GAACNNNNNNTCC 2 cut(s) 65, 97
AluBI AGCT 6 cut(s) 162, 262, 722, 837, 843, 1368
AluI AGCT 6 cut(s) 162, 262, 722, 837, 843, 1368
Alw21I GWGCWC 3 cut(s) 164, 264, 1232
Alw26I GTCTC 2 cut(s) 307, 636
AlwI GGATC 4 cut(s) 348, 700, 1181, 1276
AlwNI CAGNNNCTG 1 cut(s) 1079
Ama87I CYCGRG 2 cut(s) 68, 1202
AoxI GGCC 5 cut(s) 165, 991, 1018, 1127, 1295
ApeKI GCWGC 6 cut(s) 138, 141, 837, 840, 941, 1079
ApoI RAATTY 1 cut(s) 1165
AspLEI GCGC 2 cut(s) 815, 874
AspS9I GGNCC 3 cut(s) 776, 1018, 1262
AsuC2I CCSGG 1 cut(s) 670
AsuHPI GGTGA 4 cut(s) 451, 526, 568, 658
AsuII TTCGAA 1 cut(s) 382
AvaI CYCGRG 2 cut(s) 68, 1202
AvaII GGWCC 2 cut(s) 776, 1262
BaeGI GKGCMC 1 cut(s) 1049
BanI GGYRCC 1 cut(s) 331
BanII GRGCYC 2 cut(s) 164, 264
BbsI GAAGAC 1 cut(s) 820
Bbv12I GWGCWC 3 cut(s) 164, 264, 1232
BbvI GCAGC 6 cut(s) 150, 153, 824, 852, 928, 1066
BccI CCATC 8 cut(s) 206, 378, 518, 548, 668, 683, 1013, 1061
BceAI ACGGC 4 cut(s) 134, 786, 826, 1026
BcgI CGANNNNNNTGC 4 cut(s) 67, 101, 667, 701
BciT130I CCWGG 4 cut(s) 521, 857, 1266, 1299
BclI TGATCA 1 cut(s) 1311
BcnI CCSGG 1 cut(s) 670
BcoDI GTCTC 2 cut(s) 307, 636
BcuI ACTAGT 1 cut(s) 1277
BfaI CTAG 3 cut(s) 549, 1121, 1278
BfmI CTRYAG 4 cut(s) 832, 838, 918, 942
BglII AGATCT 1 cut(s) 175
Bme1390I CCNGG 5 cut(s) 521, 670, 857, 1266, 1299
Bme18I GGWCC 2 cut(s) 776, 1262
BmeT110I CYCGRG 2 cut(s) 68, 1202
BmgT120I GGNCC 3 cut(s) 776, 1018, 1262
BmiI GGNNCC 2 cut(s) 333, 674
BmrFI CCNGG 5 cut(s) 521, 670, 857, 1266, 1299
BmrI ACTGGG 1 cut(s) 445
BmsI GCATC 1 cut(s) 1315
BmuI ACTGGG 1 cut(s) 445
BpiI GAAGAC 1 cut(s) 820
BpmI CTGGAG 2 cut(s) 164, 498
Bpu10I CCTNAGC 1 cut(s) 1014
Bpu14I TTCGAA 1 cut(s) 382
BpuMI CCSGG 1 cut(s) 670
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 2 cut(s) 421, 1130
Bsc4I CCNNNNNNNGG 3 cut(s) 989, 1382, 1397
Bse118I RCCGGY 1 cut(s) 581
Bse1I ACTGG 2 cut(s) 440, 1402
Bse3DI GCAATG 3 cut(s) 185, 685, 943
BseBI CCWGG 4 cut(s) 521, 857, 1266, 1299
BseDI CCNNGG 2 cut(s) 421, 1130
BseGI GGATG 2 cut(s) 529, 1053
BseLI CCNNNNNNNGG 3 cut(s) 989, 1382, 1397
BseMI GCAATG 3 cut(s) 185, 685, 943
BseMII CTCAG 5 cut(s) 205, 1028, 1217, 1236, 1391
BseNI ACTGG 2 cut(s) 440, 1402
BseSI GKGCMC 1 cut(s) 1049
BseX3I CGGCCG 1 cut(s) 165
BseXI GCAGC 6 cut(s) 150, 153, 824, 852, 928, 1066
BsgI GTGCAG 1 cut(s) 1048
Bsh1236I CGCG 3 cut(s) 89, 815, 872
Bsh1285I CGRYCG 1 cut(s) 168
BshFI GGCC 5 cut(s) 167, 993, 1020, 1129, 1297
BshNI GGYRCC 1 cut(s) 331
BsiEI CGRYCG 1 cut(s) 168
BsiHKAI GWGCWC 3 cut(s) 164, 264, 1232
BsiHKCI CYCGRG 2 cut(s) 68, 1202
BsiSI CCGG 2 cut(s) 582, 669
BslFI GGGAC 3 cut(s) 76, 254, 515
BslI CCNNNNNNNGG 3 cut(s) 989, 1382, 1397
BsmAI GTCTC 2 cut(s) 307, 636
BsmBI CGTCTC 2 cut(s) 307, 636
BsmFI GGGAC 3 cut(s) 76, 254, 515
BsmI GAATGC 1 cut(s) 874
BsnI GGCC 5 cut(s) 167, 993, 1020, 1129, 1297
BsoBI CYCGRG 2 cut(s) 68, 1202
Bsp119I TTCGAA 1 cut(s) 382
Bsp1286I GDGCHC 4 cut(s) 164, 264, 1049, 1232
Bsp1407I TGTACA 2 cut(s) 284, 294
Bsp143I GATC 8 cut(s) 3, 175, 340, 636, 692, 1186, 1268, 1311
Bsp19I CCATGG 1 cut(s) 1130
BspANI GGCC 5 cut(s) 167, 993, 1020, 1129, 1297
BspCNI CTCAG 5 cut(s) 204, 1027, 1218, 1237, 1390
BspFNI CGCG 3 cut(s) 89, 815, 872
BspHI TCATGA 1 cut(s) 201
BspLI GGNNCC 2 cut(s) 333, 674
BspMAI CTGCAG 2 cut(s) 842, 946
BspPI GGATC 4 cut(s) 348, 700, 1181, 1276
BspT104I TTCGAA 1 cut(s) 382
BspT107I GGYRCC 1 cut(s) 331
BsrBI CCGCTC 1 cut(s) 274
BsrDI GCAATG 3 cut(s) 185, 685, 943
BsrFI RCCGGY 1 cut(s) 581
BsrGI TGTACA 2 cut(s) 284, 294
BsrI ACTGG 2 cut(s) 440, 1402
BssAI RCCGGY 1 cut(s) 581
BssECI CCNNGG 2 cut(s) 421, 1130
BssMI GATC 8 cut(s) 3, 175, 340, 636, 692, 1186, 1268, 1311
BssNI GRCGYC 1 cut(s) 147
BssT1I CCWWGG 1 cut(s) 1130
Bst2UI CCWGG 4 cut(s) 521, 857, 1266, 1299
Bst4CI ACNGT 9 cut(s) 35, 250, 284, 647, 919, 962, 1108, 1153, 1342
Bst6I CTCTTC 1 cut(s) 588
BstACI GRCGYC 1 cut(s) 147
BstAPI GCANNNNNTGC 1 cut(s) 1325
BstAUI TGTACA 2 cut(s) 284, 294
BstBI TTCGAA 1 cut(s) 382
BstC8I GCNNGC 2 cut(s) 792, 980
BstDEI CTNAG 7 cut(s) 191, 265, 374, 1014, 1226, 1245, 1377
BstDSI CCRYGG 2 cut(s) 421, 1130
BstEII GGTNACC 1 cut(s) 1301
BstENI CCTNNNNNAGG 1 cut(s) 1380
BstF5I GGATG 2 cut(s) 529, 1053
BstFNI CGCG 3 cut(s) 89, 815, 872
BstHHI GCGC 2 cut(s) 815, 874
BstKTI GATC 8 cut(s) 6, 178, 343, 639, 695, 1189, 1271, 1314
BstMAI GTCTC 2 cut(s) 307, 636
BstMBI GATC 8 cut(s) 3, 175, 340, 636, 692, 1186, 1268, 1311
BstMCI CGRYCG 1 cut(s) 168
BstMWI GCNNNNNNNGC 6 cut(s) 62, 457, 719, 728, 1088, 1325
BstNI CCWGG 4 cut(s) 521, 857, 1266, 1299
BstPI GGTNACC 1 cut(s) 1301
BstSCI CCNGG 5 cut(s) 519, 668, 855, 1264, 1297
BstSFI CTRYAG 4 cut(s) 832, 838, 918, 942
BstSLI GKGCMC 1 cut(s) 1049
BstUI CGCG 3 cut(s) 89, 815, 872
BstV1I GCAGC 6 cut(s) 150, 153, 824, 852, 928, 1066
BstV2I GAAGAC 1 cut(s) 820
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
BstZI CGGCCG 1 cut(s) 165
BsuRI GGCC 5 cut(s) 167, 993, 1020, 1129, 1297
BtgI CCRYGG 2 cut(s) 421, 1130
BtsCI GGATG 2 cut(s) 529, 1053
BtsIMutI CAGTG 5 cut(s) 199, 398, 1104, 1223, 1347
Cac8I GCNNGC 2 cut(s) 792, 980
CaiI CAGNNNCTG 1 cut(s) 1079
CciI TCATGA 1 cut(s) 201
CfoI GCGC 2 cut(s) 815, 874
Cfr10I RCCGGY 1 cut(s) 581
Cfr13I GGNCC 3 cut(s) 776, 1018, 1262
CseI GACGC 5 cut(s) 78, 155, 236, 289, 600
Csp6I GTAC 4 cut(s) 285, 295, 625, 868
CspCI CAANNNNNGTGG 2 cut(s) 403, 438
CviAII CATG 5 cut(s) 202, 1131, 1171, 1293, 1307
CviQI GTAC 4 cut(s) 285, 295, 625, 868
DdeI CTNAG 7 cut(s) 191, 265, 374, 1014, 1226, 1245, 1377
DpnI GATC 8 cut(s) 5, 177, 342, 638, 694, 1188, 1270, 1313
DpnII GATC 8 cut(s) 3, 175, 340, 636, 692, 1186, 1268, 1311
DrdI GACNNNNNNGTC 1 cut(s) 824
DseDI GACNNNNNNGTC 1 cut(s) 824
EaeI YGGCCR 1 cut(s) 165
EagI CGGCCG 1 cut(s) 165
Eam1104I CTCTTC 1 cut(s) 588
EarI CTCTTC 1 cut(s) 588
Ecl136II GAGCTC 2 cut(s) 162, 262
EclXI CGGCCG 1 cut(s) 165
Eco130I CCWWGG 1 cut(s) 1130
Eco24I GRGCYC 2 cut(s) 164, 264
Eco47I GGWCC 2 cut(s) 776, 1262
Eco52I CGGCCG 1 cut(s) 165
Eco53kI GAGCTC 2 cut(s) 162, 262
Eco57I CTGAAG 1 cut(s) 452
Eco88I CYCGRG 2 cut(s) 68, 1202
Eco91I GGTNACC 1 cut(s) 1301
EcoICRI GAGCTC 2 cut(s) 162, 262
EcoNI CCTNNNNNAGG 1 cut(s) 1380
EcoO109I RGGNCCY 1 cut(s) 1262
EcoO65I GGTNACC 1 cut(s) 1301
EcoRII CCWGG 4 cut(s) 519, 855, 1264, 1297
EcoT14I CCWWGG 1 cut(s) 1130
EcoT38I GRGCYC 2 cut(s) 164, 264
ErhI CCWWGG 1 cut(s) 1130
Esp3I CGTCTC 2 cut(s) 307, 636
FaeI CATG 5 cut(s) 205, 1134, 1174, 1296, 1310
FalI AAGNNNNNCTT 2 cut(s) 611, 643
FaqI GGGAC 3 cut(s) 76, 254, 515
FatI CATG 5 cut(s) 201, 1130, 1170, 1292, 1306
FauI CCCGC 1 cut(s) 279
FbaI TGATCA 1 cut(s) 1311
FblI GTMKAC 1 cut(s) 429
FokI GGATG 2 cut(s) 536, 1040
FriOI GRGCYC 2 cut(s) 164, 264
FspBI CTAG 3 cut(s) 549, 1121, 1278
GlaI GCGC 2 cut(s) 814, 873
GsuI CTGGAG 2 cut(s) 164, 498
HaeIII GGCC 5 cut(s) 167, 993, 1020, 1129, 1297
HapII CCGG 2 cut(s) 582, 669
HgaI GACGC 5 cut(s) 78, 155, 236, 289, 600
HhaI GCGC 2 cut(s) 815, 874
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 5 cut(s) 205, 1134, 1174, 1296, 1310
Hin6I GCGC 2 cut(s) 813, 872
HinP1I GCGC 2 cut(s) 813, 872
HincII GTYRAC 2 cut(s) 133, 751
HindII GTYRAC 2 cut(s) 133, 751
HinfI GANTC 4 cut(s) 707, 747, 1033, 1287
HpaI GTTAAC 1 cut(s) 133
HpaII CCGG 2 cut(s) 582, 669
HphI GGTGA 4 cut(s) 451, 526, 568, 658
Hpy166II GTNNAC 5 cut(s) 133, 318, 430, 751, 1156
Hpy188I TCNGA 9 cut(s) 159, 226, 641, 657, 806, 884, 1038, 1273, 1380
Hpy188III TCNNGA 4 cut(s) 181, 202, 1057, 1111
Hpy8I GTNNAC 5 cut(s) 133, 318, 430, 751, 1156
Hpy99I CGWCG 4 cut(s) 149, 230, 616, 689
HpyAV CCTTC 3 cut(s) 450, 728, 1129
HpyCH4III ACNGT 9 cut(s) 35, 250, 284, 647, 919, 962, 1108, 1153, 1342
HpyCH4V TGCA 8 cut(s) 78, 790, 840, 944, 1065, 1162, 1223, 1319
HpyF10VI GCNNNNNNNGC 6 cut(s) 62, 457, 719, 728, 1088, 1325
HpyF3I CTNAG 7 cut(s) 191, 265, 374, 1014, 1226, 1245, 1377
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 5 cut(s) 205, 1134, 1174, 1296, 1310
HspAI GCGC 2 cut(s) 813, 872
Ksp22I TGATCA 1 cut(s) 1311
KspAI GTTAAC 1 cut(s) 133
Kzo9I GATC 8 cut(s) 3, 175, 340, 636, 692, 1186, 1268, 1311
Lsp1109I GCAGC 6 cut(s) 150, 153, 824, 852, 928, 1066
LweI GCATC 1 cut(s) 1315
MaeI CTAG 3 cut(s) 549, 1121, 1278
MalI GATC 8 cut(s) 5, 177, 342, 638, 694, 1188, 1270, 1313
MbiI CCGCTC 1 cut(s) 274
MboI GATC 8 cut(s) 3, 175, 340, 636, 692, 1186, 1268, 1311
MboII GAAGA 4 cut(s) 185, 458, 605, 820
MflI RGATCY 1 cut(s) 175
MhlI GDGCHC 4 cut(s) 164, 264, 1049, 1232
MluCI AATT 3 cut(s) 887, 1165, 1333
MlyI GAGTC 3 cut(s) 701, 756, 1296
MmeI TCCRAC 2 cut(s) 249, 907
MseI TTAA 3 cut(s) 132, 278, 1332
MslI CAYNNNNRTG 2 cut(s) 348, 509
MspA1I CMGCKG 2 cut(s) 837, 1079
MspI CCGG 2 cut(s) 582, 669
MspR9I CCNGG 5 cut(s) 521, 670, 857, 1266, 1299
Mva1269I GAATGC 1 cut(s) 874
MvaI CCWGG 4 cut(s) 521, 857, 1266, 1299
MvnI CGCG 3 cut(s) 89, 815, 872
MwoI GCNNNNNNNGC 6 cut(s) 62, 457, 719, 728, 1088, 1325
NciI CCSGG 1 cut(s) 670
NcoI CCATGG 1 cut(s) 1130
NdeII GATC 8 cut(s) 3, 175, 340, 636, 692, 1186, 1268, 1311
NlaIII CATG 5 cut(s) 205, 1134, 1174, 1296, 1310
NlaIV GGNNCC 2 cut(s) 333, 674
NmeAIII GCCGAG 1 cut(s) 143
NmuCI GTSAC 5 cut(s) 241, 396, 574, 902, 1288
NspV TTCGAA 1 cut(s) 382
PaeR7I CTCGAG 2 cut(s) 68, 1202
PagI TCATGA 1 cut(s) 201
PcsI WCGNNNNNNNCGW 1 cut(s) 611
PctI GAATGC 1 cut(s) 874
PfeI GAWTC 1 cut(s) 1033
PleI GAGTC 3 cut(s) 701, 755, 1295
PpsI GAGTC 3 cut(s) 701, 755, 1295
PpuMI RGGWCCY 1 cut(s) 1262
Psp124BI GAGCTC 2 cut(s) 164, 264
Psp5II RGGWCCY 1 cut(s) 1262
Psp6I CCWGG 4 cut(s) 519, 855, 1264, 1297
PspEI GGTNACC 1 cut(s) 1301
PspGI CCWGG 4 cut(s) 519, 855, 1264, 1297
PspN4I GGNNCC 2 cut(s) 333, 674
PspPI GGNCC 3 cut(s) 776, 1018, 1262
PspPPI RGGWCCY 1 cut(s) 1262
PspXI VCTCGAGB 2 cut(s) 68, 1202
PsrI GAACNNNNNNTAC 2 cut(s) 608, 640
PstI CTGCAG 2 cut(s) 842, 946
PstNI CAGNNNCTG 1 cut(s) 1079
PsuI RGATCY 1 cut(s) 175
PvuII CAGCTG 1 cut(s) 837
RsaI GTAC 4 cut(s) 286, 296, 626, 869
RsaNI GTAC 4 cut(s) 285, 295, 625, 868
RseI CAYNNNNRTG 2 cut(s) 348, 509
SacI GAGCTC 2 cut(s) 164, 264
SaqAI TTAA 3 cut(s) 132, 278, 1332
Sau3AI GATC 8 cut(s) 3, 175, 340, 636, 692, 1186, 1268, 1311
Sau96I GGNCC 3 cut(s) 776, 1018, 1262
SchI GAGTC 3 cut(s) 701, 756, 1296
ScrFI CCNGG 5 cut(s) 521, 670, 857, 1266, 1299
SduI GDGCHC 4 cut(s) 164, 264, 1049, 1232
SfaNI GCATC 1 cut(s) 1315
SfcI CTRYAG 4 cut(s) 832, 838, 918, 942
Sfr274I CTCGAG 2 cut(s) 68, 1202
SfuI TTCGAA 1 cut(s) 382
SgrAI CRCCGGYG 1 cut(s) 581
SinI GGWCC 2 cut(s) 776, 1262
SlaI CTCGAG 2 cut(s) 68, 1202
SmiMI CAYNNNNRTG 2 cut(s) 348, 509
SmlI CTYRAG 2 cut(s) 68, 1202
SmoI CTYRAG 2 cut(s) 68, 1202
SpeI ACTAGT 1 cut(s) 1277
Sse9I AATT 3 cut(s) 887, 1165, 1333
SspMI CTAG 3 cut(s) 549, 1121, 1278
SstI GAGCTC 2 cut(s) 164, 264
StyD4I CCNGG 5 cut(s) 519, 668, 855, 1264, 1297
StyI CCWWGG 1 cut(s) 1130
TaaI ACNGT 9 cut(s) 35, 250, 284, 647, 919, 962, 1108, 1153, 1342
TasI AATT 3 cut(s) 887, 1165, 1333
TatI WGTACW 2 cut(s) 284, 294
TauI GCSGC 4 cut(s) 170, 463, 476, 1079
TfiI GAWTC 1 cut(s) 1033
Tru1I TTAA 3 cut(s) 132, 278, 1332
Tru9I TTAA 3 cut(s) 132, 278, 1332
TscAI CASTG 5 cut(s) 199, 405, 1111, 1230, 1347
TseFI GTSAC 5 cut(s) 241, 396, 574, 902, 1288
TseI GCWGC 6 cut(s) 138, 141, 837, 840, 941, 1079
Tsp45I GTSAC 5 cut(s) 241, 396, 574, 902, 1288
TspDTI ATGAA 4 cut(s) 97, 606, 916, 1159
TspRI CASTG 5 cut(s) 199, 405, 1111, 1230, 1347
VpaK11BI GGWCC 2 cut(s) 776, 1262
XagI CCTNNNNNAGG 1 cut(s) 1380
XapI RAATTY 1 cut(s) 1165
XhoI CTCGAG 2 cut(s) 68, 1202
XmiI GTMKAC 1 cut(s) 429
XspI CTAG 3 cut(s) 549, 1121, 1278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.