FvH4_5g35570

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000522
Physical Location & Seq
Reverse (-)
26040429 .. 26042626
2198 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35570.t1

Sequence Viewer

Length: 1698 bp
ATGGCTTCAAAGCTCTACTCTCTTGTAACCCTTTCATCAATATTGTTTCTCACCATCTTCTCTTTGCTTACCTCTCCATCTCTAGCTGTAGATGTTCTTCTAAACACTCCTCTTTCATCAGAAACCATATGCAACTCCACACCACACCCTTCTTACTGTAAATCTGTCCTCCCTCGCAAAAGCGCCAACGTCTATGACTTCGGCCGCTTTTCAGTCCAGCAGTCACTTGTTCAATCCAAAATTCTTTTGGATTTGCTCGAAAAATATCTCAGTGGCCGGCGTTCTAGTCTAACCTTACCCGCAATCCAAGCCCTAGAGGATTGTCACTTCCTTGCTTCGTTAAACATTGATTTCTTGTCACAAGCCATTGGTACCGTCGGAGAGACTAGTAATGTTCTTCCAAGCTCAGATGCTGACGATGTCCAAACCTTGCTCAGTGCCATTTTAACCAACCAGCAAACTTGTGCTGATGGCCTTAATTCTTTGGCCACTGTCCACAACGATCTCATAGTCCCACTCTCTAATGGTACAAATTTGTACAGTGTCTCTTTAGCTTTGTTTACAAAGGGTTGGGTGCCAAAGGGTGGCAAAAATAATGTGACACCAGGTCAATCCTACAGGCATCTCAATTTCAGGAAGGGGCGATTGTCACTGAAAATGTCGAGCAAAGTGCGTGCAGTTTACGATTCAGTTATAAATCGAAGAAGGCACCTTCTTCAGGCCGGAGATGAAGAAGTTGTGGTGAAGGACATTGTGGTTGTGAGCCAAGATGGAACCGGAAACTTCACTACCATCAATGATGCTATCAATGCTGCACCTAATAACTCCGTTGCTAGTGATGGTTACTTCTTGGTATATATCACTGCTGGTGTTTATCAAGAGTACGTTTCGATTTCATCAAAAAAGAAGTACTTGCTTATGATTGGAGATGGTATCAACCAGACAATCATCACAGGAAACCACAGCGTCGGAGACGGATGGACTACTTTCAACTCTCCCACTTTTGCTGTGGCGGCTCAAGGATTTGTAGCAGTAAACATTACTTTTCAAAACAAGGCCGGACCAAGCAAGGGGCAGGCAGTGGCAGTTAGAAACGATGGCGATTTGTCGGTTTTCTATAGCTGCAGCTTTGAAGCCTACCAAGACACCTTATACACACATTCCATGAGACAATTCTACAGAGAATGTGATATCTATGGAACTGTTGATTTCATATTCGGCAATGCTGCTGTTGCTTTCCAAAACTGCAACATCTACCCCCGTCGTCCCCTCCAAGGCCAATCAAATACCATCACTGCTCAAGGGCGGACCGACGTGAATCAGAATACCGGGATTTCAATCCAGAATTGCACAGTTACAGCGGCAGCTGATCTCGCTTCGAGTACTTTTACTGTCAAGAACTATCTGGGGAGGCCATGGAAGGAATACTCGAGGACAATTTTCATGCAGGGTTTCATCGATAGTTTCATAGATCCTGCAGGTTGGCTTGCGTGGAATGGAGACTTTGCACTGAGCACATTGTATTATGCTGAGTATAATAATAGTGGACCTGGATCAAACACTAACAACAGAGTTACATGGCCTGGTTACCATGTCATCGGTGCAACTGAGGCCGCCAACTTTACAGTCTCCAACTTCATATTGGGGGATAATTGGCTGCCTCAGACAGGAGTGCCTTACACTGGTGGCTTAATTTGA

Protein Analysis

566

Amino Acids

61.55

Weight (kDa)

6.45

Isoelectric Point (pI)

35.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 41 - 186 6.2e-24 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 252 - 550 7.2e-137 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000522)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02810 AT2G47550 AT4G02330
fragaria_vesca FvH4_2g25960 FvH4_2g25960 FvH4_2g25970 FvH4_5g35570 FvH4_5g35580 FvH4_5g35590
malus_domestica MD03G1290900.v1.1 MD03G1291000.v1.1 MD08G1195600.v1.1 MD11G1307400.v1.1 MD11G1307500.v1.1
prunus_persica Prupe.1G529400_v2.0.a1 Prupe.1G529500_v2.0.a1 Prupe.8G263900_v2.0.a1 Prupe.8G264000_v2.0.a1
pyrus_communis pycom08g16800
rosa_chinensis RchiOBHm_Chr6g0294321 RchiOBHm_Chr6g0294331 RchiOBHm_Chr7g0236951 RchiOBHm_Chr7g0236971 RchiOBHm_Chr7g0237041 RchiOBHm_Chr7g0237051
rosa_laevigata RLG00000001034 RLG00000001035 RLG00000001039 RLG00000001043 RLG00000001045 RLG00000011880 RLG00000011881 RLG00000034181
rosa_multiflora Rmu_co8086188.1_g000001 Rmu_co8458485.1_g000001 Rmu_sc0000272.1_g000007 Rmu_sc0000272.1_g000009 Rmu_sc0002131.1_g000030 Rmu_sc0002131.1_g000037 Rmu_sc0005369.1_g000005 Rmu_sc0005369.1_g000008 Rmu_sc0007991.1_g000005 Rmu_sc0007991.1_g000013 Rmu_sc0033563.1_g000001
rosa_roxburghii Rroxscaffold_3G00224540 Rroxscaffold_3G00224600 Rroxscaffold_3G00224650 Rroxscaffold_3G00224660 Rroxscaffold_7G00173470 Rroxscaffold_7G00173480
rosa_rugosa Rorug06G0243400 Rorug06G0243500 Rorug07G0301200 Rorug07G0301500 Rorug07G0301600
rosa_samantha Rh6AG356000 Rh6AG356100 Rh6BG362600 Rh6BG362800 Rh6CG370000 Rh6CG370100 Rh6DG356500 Rh6DG356600 Rh7AG456200 Rh7AG456500 Rh7AG457500 Rh7AG457600 Rh7BG427500 Rh7BG427700 Rh7BG428400 Rh7BG428500 Rh7CG475100 Rh7CG475300 Rh7CG475700 Rh7CG475800 Rh7DG444100 Rh7DG444300 Rh7DG444700 Rh7DG444900
rosa_wichuraiana Rw6G031020 Rw6G031030 Rw7G037870 Rw7G037880 Rw7G037920 Rw7G037940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 695
Acc36I ACCTGC 1 cut(s) 1469
Acc65I GGTACC 1 cut(s) 371
AccB1I GGYRCC 3 cut(s) 371, 574, 708
AccB7I CCANNNNNTGG 1 cut(s) 584
AciI CCGC 6 cut(s) 205, 300, 1013, 1306, 1361, 1614
AclWI GGATC 2 cut(s) 1466, 1561
AcoI YGGCCR 3 cut(s) 202, 274, 486
AcsI RAATTY 2 cut(s) 240, 532
AcuI CTGAAG 1 cut(s) 701
AfaI GTAC 6 cut(s) 373, 529, 539, 884, 911, 1384
AfiI CCNNNNNNNGG 6 cut(s) 584, 718, 1070, 1274, 1667, 1682
AgsI TTSAA 6 cut(s) 9, 233, 991, 1049, 1133, 1338
AhdI GACNNNNNGTC 1 cut(s) 606
AhlI ACTAGT 1 cut(s) 386
AjiI CACGTC 1 cut(s) 1315
AjnI CCWGG 3 cut(s) 604, 1549, 1582
AluBI AGCT 7 cut(s) 13, 86, 405, 554, 1122, 1128, 1367
AluI AGCT 7 cut(s) 13, 86, 405, 554, 1122, 1128, 1367
Alw21I GWGCWC 1 cut(s) 1517
Alw26I GTCTC 6 cut(s) 377, 550, 966, 1162, 1494, 1633
AlwI GGATC 2 cut(s) 1466, 1561
AlwNI CAGNNNCTG 1 cut(s) 413
Ama87I CYCGRG 1 cut(s) 1429
ApeKI GCWGC 6 cut(s) 812, 1122, 1125, 1226, 1364, 1657
ApoI RAATTY 2 cut(s) 240, 532
Asp718I GGTACC 1 cut(s) 371
AspLEI GCGC 1 cut(s) 185
AspS9I GGNCC 3 cut(s) 1061, 1308, 1547
AsuC2I CCSGG 1 cut(s) 1330
AsuHPI GGTGA 2 cut(s) 43, 754
AvaI CYCGRG 1 cut(s) 1429
AvaII GGWCC 3 cut(s) 1061, 1308, 1547
BalI TGGCCA 1 cut(s) 488
BanI GGYRCC 3 cut(s) 371, 574, 708
BarI GAAGNNNNNNTAC 2 cut(s) 81, 113
Bbv12I GWGCWC 1 cut(s) 1517
BbvI GCAGC 6 cut(s) 799, 1109, 1137, 1213, 1376, 1644
BcgI CGANNNNNNTGC 2 cut(s) 652, 686
BciT130I CCWGG 3 cut(s) 606, 1551, 1584
BcnI CCSGG 1 cut(s) 1330
BcoDI GTCTC 6 cut(s) 377, 550, 966, 1162, 1494, 1633
BcuI ACTAGT 1 cut(s) 386
BfaI CTAG 5 cut(s) 83, 285, 314, 387, 834
BfmI CTRYAG 6 cut(s) 87, 616, 1117, 1123, 1177, 1476
BfoI RGCGCY 1 cut(s) 186
BfuAI ACCTGC 1 cut(s) 1469
BmcAI AGTACT 2 cut(s) 911, 1384
Bme1390I CCNGG 4 cut(s) 606, 1330, 1551, 1584
Bme18I GGWCC 3 cut(s) 1061, 1308, 1547
BmeRI GACNNNNNGTC 1 cut(s) 606
BmeT110I CYCGRG 1 cut(s) 1429
BmgBI CACGTC 1 cut(s) 1315
BmgT120I GGNCC 3 cut(s) 1061, 1308, 1547
BmiI GGNNCC 4 cut(s) 373, 576, 710, 775
BmrFI CCNGG 4 cut(s) 606, 1330, 1551, 1584
BmsI GCATC 3 cut(s) 400, 631, 790
BpuEI CTTGAG 2 cut(s) 1002, 1284
BpuMI CCSGG 1 cut(s) 1330
Bsa29I ATCGAT 1 cut(s) 1458
BsaBI GATNNNNATC 1 cut(s) 1337
BsaJI CCNNGG 2 cut(s) 1273, 1415
BsaWI WCCGGW 1 cut(s) 776
Bsc4I CCNNNNNNNGG 6 cut(s) 584, 718, 1070, 1274, 1667, 1682
Bse118I RCCGGY 1 cut(s) 276
Bse1I ACTGG 1 cut(s) 1687
Bse3DI GCAATG 1 cut(s) 1228
Bse8I GATNNNNATC 1 cut(s) 1337
BseBI CCWGG 3 cut(s) 606, 1551, 1584
BseCI ATCGAT 1 cut(s) 1458
BseDI CCNNGG 2 cut(s) 1273, 1415
BseGI GGATG 1 cut(s) 983
BseJI GATNNNNATC 1 cut(s) 1337
BseLI CCNNNNNNNGG 6 cut(s) 584, 718, 1070, 1274, 1667, 1682
BseMI GCAATG 1 cut(s) 1228
BseMII CTCAG 7 cut(s) 283, 420, 448, 1502, 1521, 1599, 1676
BseNI ACTGG 1 cut(s) 1687
BseRI GAGGAG 1 cut(s) 99
BseX3I CGGCCG 1 cut(s) 202
BseXI GCAGC 6 cut(s) 799, 1109, 1137, 1213, 1376, 1644
BsgI GTGCAG 2 cut(s) 696, 798
Bsh1285I CGRYCG 1 cut(s) 205
BshNI GGYRCC 3 cut(s) 371, 574, 708
BshVI ATCGAT 1 cut(s) 1458
BsiEI CGRYCG 1 cut(s) 205
BsiHKAI GWGCWC 1 cut(s) 1517
BsiHKCI CYCGRG 1 cut(s) 1429
BsiSI CCGG 5 cut(s) 277, 723, 777, 1059, 1329
BslFI GGGAC 2 cut(s) 497, 1251
BslI CCNNNNNNNGG 6 cut(s) 584, 718, 1070, 1274, 1667, 1682
BsmAI GTCTC 6 cut(s) 377, 550, 966, 1162, 1494, 1633
BsmBI CGTCTC 1 cut(s) 966
BsmFI GGGAC 2 cut(s) 497, 1251
BsoBI CYCGRG 1 cut(s) 1429
Bsp1286I GDGCHC 1 cut(s) 1517
Bsp1407I TGTACA 1 cut(s) 537
Bsp143I GATC 4 cut(s) 502, 1369, 1471, 1553
Bsp19I CCATGG 1 cut(s) 1415
BspACI CCGC 6 cut(s) 205, 300, 1013, 1306, 1361, 1614
BspCNI CTCAG 7 cut(s) 282, 419, 447, 1503, 1522, 1600, 1675
BspDI ATCGAT 1 cut(s) 1458
BspLI GGNNCC 4 cut(s) 373, 576, 710, 775
BspMAI CTGCAG 2 cut(s) 1127, 1480
BspMI ACCTGC 1 cut(s) 1469
BspPI GGATC 2 cut(s) 1466, 1561
BspT107I GGYRCC 3 cut(s) 371, 574, 708
BsrDI GCAATG 1 cut(s) 1228
BsrFI RCCGGY 1 cut(s) 276
BsrGI TGTACA 1 cut(s) 537
BsrI ACTGG 1 cut(s) 1687
BssAI RCCGGY 1 cut(s) 276
BssECI CCNNGG 2 cut(s) 1273, 1415
BssMI GATC 4 cut(s) 502, 1369, 1471, 1553
BssT1I CCWWGG 2 cut(s) 1273, 1415
Bst2UI CCWGG 3 cut(s) 606, 1551, 1584
Bst4CI ACNGT 8 cut(s) 158, 376, 493, 542, 1204, 1354, 1393, 1627
BstAUI TGTACA 1 cut(s) 537
BstC8I GCNNGC 4 cut(s) 278, 675, 1077, 1488
BstDEI CTNAG 7 cut(s) 269, 406, 434, 1511, 1530, 1608, 1662
BstDSI CCRYGG 1 cut(s) 1415
BstEII GGTNACC 1 cut(s) 1586
BstENI CCTNNNNNAGG 2 cut(s) 716, 1665
BstF5I GGATG 1 cut(s) 983
BstH2I RGCGCY 1 cut(s) 186
BstHHI GCGC 1 cut(s) 185
BstKTI GATC 4 cut(s) 505, 1372, 1474, 1556
BstMAI GTCTC 6 cut(s) 377, 550, 966, 1162, 1494, 1633
BstMBI GATC 4 cut(s) 502, 1369, 1471, 1553
BstMCI CGRYCG 1 cut(s) 205
BstMWI GCNNNNNNNGC 6 cut(s) 308, 809, 1013, 1232, 1373, 1610
BstNI CCWGG 3 cut(s) 606, 1551, 1584
BstPI GGTNACC 1 cut(s) 1586
BstSCI CCNGG 4 cut(s) 604, 1328, 1549, 1582
BstSFI CTRYAG 6 cut(s) 87, 616, 1117, 1123, 1177, 1476
BstV1I GCAGC 6 cut(s) 799, 1109, 1137, 1213, 1376, 1644
BstX2I RGATCY 1 cut(s) 1471
BstYI RGATCY 1 cut(s) 1471
BstZI CGGCCG 1 cut(s) 202
Bsu15I ATCGAT 1 cut(s) 1458
BsuTUI ATCGAT 1 cut(s) 1458
BtgI CCRYGG 1 cut(s) 1415
BtrI CACGTC 1 cut(s) 1315
BtsCI GGATG 1 cut(s) 983
BtsI GCAGTG 3 cut(s) 861, 1086, 1293
BveI ACCTGC 1 cut(s) 1469
Cac8I GCNNGC 4 cut(s) 278, 675, 1077, 1488
CaiI CAGNNNCTG 1 cut(s) 413
CfoI GCGC 1 cut(s) 185
Cfr10I RCCGGY 1 cut(s) 276
Cfr13I GGNCC 3 cut(s) 1061, 1308, 1547
ClaI ATCGAT 1 cut(s) 1458
CpoI CGGWCCG 1 cut(s) 1308
CseI GACGC 1 cut(s) 955
CsiI ACCWGGT 1 cut(s) 604
Csp6I GTAC 6 cut(s) 372, 528, 538, 883, 910, 1383
CspI CGGWCCG 1 cut(s) 1308
CviAII CATG 5 cut(s) 1165, 1416, 1444, 1578, 1592
CviQI GTAC 6 cut(s) 372, 528, 538, 883, 910, 1383
DdeI CTNAG 7 cut(s) 269, 406, 434, 1511, 1530, 1608, 1662
DpnI GATC 4 cut(s) 504, 1371, 1473, 1555
DpnII GATC 4 cut(s) 502, 1369, 1471, 1553
DriI GACNNNNNGTC 1 cut(s) 606
EaeI YGGCCR 3 cut(s) 202, 274, 486
EagI CGGCCG 1 cut(s) 202
Eam1105I GACNNNNNGTC 1 cut(s) 606
EciI GGCGGA 1 cut(s) 1321
EclXI CGGCCG 1 cut(s) 202
Eco130I CCWWGG 2 cut(s) 1273, 1415
Eco32I GATATC 1 cut(s) 1192
Eco47I GGWCC 3 cut(s) 1061, 1308, 1547
Eco52I CGGCCG 1 cut(s) 202
Eco57I CTGAAG 1 cut(s) 701
Eco88I CYCGRG 1 cut(s) 1429
Eco91I GGTNACC 1 cut(s) 1586
EcoNI CCTNNNNNAGG 2 cut(s) 716, 1665
EcoO65I GGTNACC 1 cut(s) 1586
EcoRII CCWGG 3 cut(s) 604, 1549, 1582
EcoRV GATATC 1 cut(s) 1192
EcoT14I CCWWGG 2 cut(s) 1273, 1415
ErhI CCWWGG 2 cut(s) 1273, 1415
Esp3I CGTCTC 1 cut(s) 966
FaeI CATG 5 cut(s) 1168, 1419, 1447, 1581, 1595
FalI AAGNNNNNCTT 2 cut(s) 896, 928
FaqI GGGAC 2 cut(s) 497, 1251
FatI CATG 5 cut(s) 1164, 1415, 1443, 1577, 1591
FauI CCCGC 1 cut(s) 307
FauNDI CATATG 1 cut(s) 128
FokI GGATG 1 cut(s) 990
FspBI CTAG 5 cut(s) 83, 285, 314, 387, 834
GlaI GCGC 1 cut(s) 184
HaeII RGCGCY 1 cut(s) 186
HapII CCGG 5 cut(s) 277, 723, 777, 1059, 1329
HgaI GACGC 1 cut(s) 955
HhaI GCGC 1 cut(s) 185
Hin1II CATG 5 cut(s) 1168, 1419, 1447, 1581, 1595
Hin6I GCGC 1 cut(s) 183
HinP1I GCGC 1 cut(s) 183
HinfI GANTC 2 cut(s) 686, 1318
HpaII CCGG 5 cut(s) 277, 723, 777, 1059, 1329
HphI GGTGA 2 cut(s) 43, 754
Hpy166II GTNNAC 5 cut(s) 496, 561, 682, 1036, 1547
Hpy188I TCNGA 6 cut(s) 121, 380, 409, 971, 1323, 1665
Hpy188III TCNNGA 4 cut(s) 634, 878, 1342, 1396
Hpy8I GTNNAC 5 cut(s) 496, 561, 682, 1036, 1547
Hpy99I CGWCG 4 cut(s) 380, 971, 1266, 1316
HpyAV CCTTC 6 cut(s) 159, 631, 699, 722, 739, 1414
HpyCH4III ACNGT 8 cut(s) 158, 376, 493, 542, 1204, 1354, 1393, 1627
HpyCH4IV ACGT 3 cut(s) 189, 885, 1314
HpyF10VI GCNNNNNNNGC 6 cut(s) 308, 809, 1013, 1232, 1373, 1610
HpyF3I CTNAG 7 cut(s) 269, 406, 434, 1511, 1530, 1608, 1662
HpySE526I ACGT 3 cut(s) 189, 885, 1314
Hsp92II CATG 5 cut(s) 1168, 1419, 1447, 1581, 1595
HspAI GCGC 1 cut(s) 183
KpnI GGTACC 1 cut(s) 375
KroI GCCGGC 1 cut(s) 276
KroNI GCCGGC 1 cut(s) 278
Kzo9I GATC 4 cut(s) 502, 1369, 1471, 1553
Lsp1109I GCAGC 6 cut(s) 799, 1109, 1137, 1213, 1376, 1644
LweI GCATC 3 cut(s) 400, 631, 790
MabI ACCWGGT 1 cut(s) 604
MaeI CTAG 5 cut(s) 83, 285, 314, 387, 834
MaeII ACGT 3 cut(s) 189, 885, 1314
MalI GATC 4 cut(s) 504, 1371, 1473, 1555
MboI GATC 4 cut(s) 502, 1369, 1471, 1553
MboII GAAGA 6 cut(s) 49, 89, 389, 707, 714, 743
MflI RGATCY 1 cut(s) 1471
MhlI GDGCHC 1 cut(s) 1517
MlsI TGGCCA 1 cut(s) 488
MluCI AATT 9 cut(s) 240, 478, 532, 628, 1172, 1345, 1437, 1651, 1692
MluNI TGGCCA 1 cut(s) 488
MmeI TCCRAC 3 cut(s) 358, 949, 1656
Mox20I TGGCCA 1 cut(s) 488
MroNI GCCGGC 1 cut(s) 276
MscI TGGCCA 1 cut(s) 488
MseI TTAA 4 cut(s) 341, 446, 477, 1691
Msp20I TGGCCA 1 cut(s) 488
MspA1I CMGCKG 2 cut(s) 1361, 1367
MspI CCGG 5 cut(s) 277, 723, 777, 1059, 1329
MspR9I CCNGG 4 cut(s) 606, 1330, 1551, 1584
MvaI CCWGG 3 cut(s) 606, 1551, 1584
MwoI GCNNNNNNNGC 6 cut(s) 308, 809, 1013, 1232, 1373, 1610
NaeI GCCGGC 1 cut(s) 278
NciI CCSGG 1 cut(s) 1330
NcoI CCATGG 1 cut(s) 1415
NdeI CATATG 1 cut(s) 128
NdeII GATC 4 cut(s) 502, 1369, 1471, 1553
NgoMIV GCCGGC 1 cut(s) 276
NlaIII CATG 5 cut(s) 1168, 1419, 1447, 1581, 1595
NlaIV GGNNCC 4 cut(s) 373, 576, 710, 775
NmuCI GTSAC 5 cut(s) 222, 323, 357, 598, 648
PaeR7I CTCGAG 1 cut(s) 1429
PdiI GCCGGC 1 cut(s) 278
PfeI GAWTC 2 cut(s) 686, 1318
PflFI GACNNNGTC 1 cut(s) 419
PflMI CCANNNNNTGG 1 cut(s) 584
PsiI TTATAA 1 cut(s) 695
Psp6I CCWGG 3 cut(s) 604, 1549, 1582
PspEI GGTNACC 1 cut(s) 1586
PspGI CCWGG 3 cut(s) 604, 1549, 1582
PspN4I GGNNCC 4 cut(s) 373, 576, 710, 775
PspPI GGNCC 3 cut(s) 1061, 1308, 1547
PspXI VCTCGAGB 1 cut(s) 1429
PstI CTGCAG 2 cut(s) 1127, 1480
PstNI CAGNNNCTG 1 cut(s) 413
PsuI RGATCY 1 cut(s) 1471
PsyI GACNNNGTC 1 cut(s) 419
PvuII CAGCTG 1 cut(s) 1367
RsaI GTAC 6 cut(s) 373, 529, 539, 884, 911, 1384
RsaNI GTAC 6 cut(s) 372, 528, 538, 883, 910, 1383
Rsr2I CGGWCCG 1 cut(s) 1308
RsrII CGGWCCG 1 cut(s) 1308
SaqAI TTAA 4 cut(s) 341, 446, 477, 1691
Sau3AI GATC 4 cut(s) 502, 1369, 1471, 1553
Sau96I GGNCC 3 cut(s) 1061, 1308, 1547
SbfI CCTGCAGG 1 cut(s) 1480
ScaI AGTACT 2 cut(s) 911, 1384
ScrFI CCNGG 4 cut(s) 606, 1330, 1551, 1584
SdaI CCTGCAGG 1 cut(s) 1480
SduI GDGCHC 1 cut(s) 1517
SexAI ACCWGGT 1 cut(s) 604
SfaNI GCATC 3 cut(s) 400, 631, 790
SfcI CTRYAG 6 cut(s) 87, 616, 1117, 1123, 1177, 1476
Sfr274I CTCGAG 1 cut(s) 1429
SinI GGWCC 3 cut(s) 1061, 1308, 1547
SlaI CTCGAG 1 cut(s) 1429
SmlI CTYRAG 3 cut(s) 1017, 1299, 1429
SmoI CTYRAG 3 cut(s) 1017, 1299, 1429
SpeI ACTAGT 1 cut(s) 386
Sse8387I CCTGCAGG 1 cut(s) 1480
Sse9I AATT 9 cut(s) 240, 478, 532, 628, 1172, 1345, 1437, 1651, 1692
SsiI CCGC 6 cut(s) 205, 300, 1013, 1306, 1361, 1614
SspI AATATT 1 cut(s) 42
SspMI CTAG 5 cut(s) 83, 285, 314, 387, 834
StyD4I CCNGG 4 cut(s) 604, 1328, 1549, 1582
StyI CCWWGG 2 cut(s) 1273, 1415
TaaI ACNGT 8 cut(s) 158, 376, 493, 542, 1204, 1354, 1393, 1627
TaiI ACGT 3 cut(s) 192, 888, 1317
TaqI TCGA 7 cut(s) 258, 662, 700, 890, 1379, 1430, 1458
TaqII GACCGA 1 cut(s) 1325
TasI AATT 9 cut(s) 240, 478, 532, 628, 1172, 1345, 1437, 1651, 1692
TatI WGTACW 3 cut(s) 537, 909, 1382
TauI GCSGC 4 cut(s) 207, 1016, 1364, 1616
TfiI GAWTC 2 cut(s) 686, 1318
Tru1I TTAA 4 cut(s) 341, 446, 477, 1691
Tru9I TTAA 4 cut(s) 341, 446, 477, 1691
TseFI GTSAC 5 cut(s) 222, 323, 357, 598, 648
TseI GCWGC 6 cut(s) 812, 1122, 1125, 1226, 1364, 1657
Tsp45I GTSAC 5 cut(s) 222, 323, 357, 598, 648
TspDTI ATGAA 9 cut(s) 24, 105, 744, 885, 1201, 1432, 1444, 1456, 1627
TspGWI ACGGA 2 cut(s) 817, 990
Tth111I GACNNNGTC 1 cut(s) 419
Van91I CCANNNNNTGG 1 cut(s) 584
VpaK11BI GGWCC 3 cut(s) 1061, 1308, 1547
XagI CCTNNNNNAGG 2 cut(s) 716, 1665
XapI RAATTY 2 cut(s) 240, 532
XcmI CCANNNNNNNNNTGG 3 cut(s) 244, 1006, 1639
XhoI CTCGAG 1 cut(s) 1429
XspI CTAG 5 cut(s) 83, 285, 314, 387, 834
ZrmI AGTACT 2 cut(s) 911, 1384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.