FvH4_5g35590

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000522
Physical Location & Seq
Forward (+)
26052228 .. 26054518
2291 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35590.t1

Sequence Viewer

Length: 1728 bp
ATGACCTATTACACATTCTCATCCTCCATTACCACTAACATGGCTTCTACTAAGCTCTTCAATCCTCTTTCACTATTCCTCATTCTCTTCTCTTTACTCATCTCTCATTCTTCATCTGCCGACGTTCCTCTCAACACTTCTCTCCCGCCGGAAACCATCTGCAACTCCACCCCACACCCTTCTTACTGCTTATCCGTCCTCCCTCACAAAAATGCCAACGTCTACGAGTTCGGACGCTTCTCCGTCAACCACTCTCTCCTCCAATCCCAAAACCTAATGGTCTCCCTCAACCAGTACCTCCAGAGCTACCGTTCCTCTTTATCACAAACCGCCATTCAAGCCCTCGAGGACTGCAAGTTTCTCGCGTCCATGAACATCGACTTCATCTCAAACACCATGCAAAAGGTTAACACCACCGGCGACGCGGTGCTTCCGAGCTCCGATGCCGATGATCTCCAGACATTGCTCAGTGCCATCATGACAAACCAACAGACTTGTTCAGACGCTCTCCGATCTGTCACTACTGTCCAAGACGAGCTCTTAGTCCCGCTACTCAACTGTACAAGCATGTACAGCGTCTCTTTGGCTCTGTTCACACAGGGTTGGGTGCCAAAGGGTCACAAGAACCCTAACGGGACGCCGAACCATCTCAAGTTTCGAAAGGGGAGGTTGTCACTGAAAATGTCAAGCAAAGCGCGTGGAGTGTACGACTGGGTGATAAGCCGGCCGGGAAGGCGGCTTCTTCAGGCTGCCGGAGATGAGGAAGTTGTGGTTAAGGACATTGTGGTGGTGAGCAAAGATGGAAGTGGGAACTTCACCACCATCAATGATGCTATTACTGCTGCGCCGAATAACTCTGTTGCTAGTGATGGGTATTTCTTGGTATATGTCACTGCCGGTGTCTATGAAGAGTATGTTTCGATTGCGTCGAACAAGCAGTACTTGTTGCTGATCGGAGATGGTATTAATCAGACCATCATCACTGGAAACCGCAATGTCGTGGATGGATCGACTACTTTCGACTCTGCTACTCTAGCTGTGGTAGCAGAAGGGTTTGTGGGAGTCAACATAACTGTTCGCAACACGGCTGGACCAAGTAAAATGCAGGCCGTGGCGGTCCGGAACGGCGCCGACTTGTCGGTCTTCTACAGCTGCAGCTTTGAGGCCTACCAGGACACCCTATACGCGCATTCTCTCCGACAATTCTACCGAGAATGCGACATCTATGGAACTGTGGACTTCATATTTGGCAATGCTGCACTTGTTTTACAAAACTGTAACCTCTACCCTCGGCAGCCACTCAAAGGCCAATCCAATCCCATCACGGCACAGGGCAGAACCGACCCGAATCAAAACACCGGAACTTCCATCCAGAACTGCACGATTACAGCCGCTGCGGACTTGGCTTCGAGTAACTTCACTGTCAAGACATATCTAGGGAGGCCATGGAAGGAGTACTCGAGGACGGTTTACTTGCAGAATTTCATGGATAGTTTGATTGATCCAGTTGGTTGGCTCGAGTGGGATGGAGATTTTGCACTGAGCACATTGTATTATGCTGAGTATGATAATAGAGGACCTGGATCAAACACTAGTAGCAGAGTTACATGGCCTGGTTACCATATAATCGGTGCAACTGATGCTGCAAATTTTACAGTGTCCAACTTTCTGGTCGGAGATAATTGGTTGCCTCAAACAGGTGTGCCTTATACCGGTGGCTTAGTTTGA

Protein Analysis

576

Amino Acids

62.88

Weight (kDa)

5.52

Isoelectric Point (pI)

34.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 51 - 197 9.8e-22 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 262 - 560 2e-136 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000522)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02810 AT2G47550 AT4G02330
fragaria_vesca FvH4_2g25960 FvH4_2g25960 FvH4_2g25970 FvH4_5g35570 FvH4_5g35580 FvH4_5g35590
malus_domestica MD03G1290900.v1.1 MD03G1291000.v1.1 MD08G1195600.v1.1 MD11G1307400.v1.1 MD11G1307500.v1.1
prunus_persica Prupe.1G529400_v2.0.a1 Prupe.1G529500_v2.0.a1 Prupe.8G263900_v2.0.a1 Prupe.8G264000_v2.0.a1
pyrus_communis pycom08g16800
rosa_chinensis RchiOBHm_Chr6g0294321 RchiOBHm_Chr6g0294331 RchiOBHm_Chr7g0236951 RchiOBHm_Chr7g0236971 RchiOBHm_Chr7g0237041 RchiOBHm_Chr7g0237051
rosa_laevigata RLG00000001034 RLG00000001035 RLG00000001039 RLG00000001043 RLG00000001045 RLG00000011880 RLG00000011881 RLG00000034181
rosa_multiflora Rmu_co8086188.1_g000001 Rmu_co8458485.1_g000001 Rmu_sc0000272.1_g000007 Rmu_sc0000272.1_g000009 Rmu_sc0002131.1_g000030 Rmu_sc0002131.1_g000037 Rmu_sc0005369.1_g000005 Rmu_sc0005369.1_g000008 Rmu_sc0007991.1_g000005 Rmu_sc0007991.1_g000013 Rmu_sc0033563.1_g000001
rosa_roxburghii Rroxscaffold_3G00224540 Rroxscaffold_3G00224600 Rroxscaffold_3G00224650 Rroxscaffold_3G00224660 Rroxscaffold_7G00173470 Rroxscaffold_7G00173480
rosa_rugosa Rorug06G0243400 Rorug06G0243500 Rorug07G0301200 Rorug07G0301500 Rorug07G0301600
rosa_samantha Rh6AG356000 Rh6AG356100 Rh6BG362600 Rh6BG362800 Rh6CG370000 Rh6CG370100 Rh6DG356500 Rh6DG356600 Rh7AG456200 Rh7AG456500 Rh7AG457500 Rh7AG457600 Rh7BG427500 Rh7BG427700 Rh7BG428400 Rh7BG428500 Rh7CG475100 Rh7CG475300 Rh7CG475700 Rh7CG475800 Rh7DG444100 Rh7DG444300 Rh7DG444700 Rh7DG444900
rosa_wichuraiana Rw6G031020 Rw6G031030 Rw7G037870 Rw7G037880 Rw7G037920 Rw7G037940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1139
AbsI CCTCGAGG 1 cut(s) 344
AccB1I GGYRCC 2 cut(s) 607, 1127
AccI GTMKAC 1 cut(s) 222
AccII CGCG 4 cut(s) 365, 425, 697, 1187
AccIII TCCGGA 1 cut(s) 1119
AciI CCGC 9 cut(s) 146, 330, 425, 548, 736, 991, 1115, 1392, 1397
AclWI GGATC 3 cut(s) 1015, 1496, 1591
AcoI YGGCCR 1 cut(s) 725
AcsI RAATTY 2 cut(s) 1480, 1648
AcuI CTGAAG 1 cut(s) 728
AcyI GRCGYC 2 cut(s) 638, 1128
AfaI GTAC 6 cut(s) 296, 562, 572, 707, 941, 1457
AfiI CCNNNNNNNGG 3 cut(s) 1304, 1697, 1712
AgeI ACCGGT 1 cut(s) 1712
AgsI TTSAA 2 cut(s) 61, 338
AhlI ACTAGT 1 cut(s) 1592
AjnI CCWGG 3 cut(s) 1170, 1579, 1612
AluBI AGCT 7 cut(s) 55, 306, 438, 538, 1037, 1152, 1158
AluI AGCT 7 cut(s) 55, 306, 438, 538, 1037, 1152, 1158
Alw21I GWGCWC 3 cut(s) 440, 540, 1547
Alw26I GTCTC 2 cut(s) 286, 583
AlwI GGATC 3 cut(s) 1015, 1496, 1591
AlwNI CAGNNNCTG 1 cut(s) 1394
Ama87I CYCGRG 3 cut(s) 344, 1459, 1517
Aor13HI TCCGGA 1 cut(s) 1119
AoxI GGCC 6 cut(s) 725, 1107, 1164, 1306, 1442, 1610
ApeKI GCWGC 8 cut(s) 749, 842, 1152, 1155, 1256, 1294, 1394, 1643
ApoI RAATTY 2 cut(s) 1480, 1648
ArsI GACNNNNNNTTYG 2 cut(s) 1229, 1261
AseI ATTAAT 1 cut(s) 966
AsiGI ACCGGT 1 cut(s) 1712
AspLEI GCGC 4 cut(s) 697, 847, 1130, 1189
AspS9I GGNCC 3 cut(s) 1091, 1117, 1577
AsuC2I CCSGG 1 cut(s) 729
AsuHPI GGTGA 3 cut(s) 727, 802, 808
AsuII TTCGAA 1 cut(s) 658
AvaI CYCGRG 3 cut(s) 344, 1459, 1517
AvaII GGWCC 3 cut(s) 1091, 1117, 1577
BanI GGYRCC 2 cut(s) 607, 1127
BanII GRGCYC 2 cut(s) 440, 540
BbsI GAAGAC 1 cut(s) 1135
Bbv12I GWGCWC 3 cut(s) 440, 540, 1547
BbvI GCAGC 8 cut(s) 736, 829, 1139, 1167, 1243, 1306, 1381, 1630
BceAI ACGGC 4 cut(s) 1094, 1101, 1141, 1341
BcgI CGANNNNNNTGC 2 cut(s) 343, 377
BciT130I CCWGG 3 cut(s) 1172, 1581, 1614
BcnI CCSGG 1 cut(s) 729
BcoDI GTCTC 2 cut(s) 286, 583
BcuI ACTAGT 1 cut(s) 1592
BfaI CTAG 4 cut(s) 864, 1034, 1436, 1593
BfmI CTRYAG 2 cut(s) 1147, 1153
BfoI RGCGCY 1 cut(s) 1131
BglI GCCNNNNNGGC 1 cut(s) 733
BmcAI AGTACT 2 cut(s) 941, 1457
Bme1390I CCNGG 4 cut(s) 729, 1172, 1581, 1614
Bme18I GGWCC 3 cut(s) 1091, 1117, 1577
BmeT110I CYCGRG 3 cut(s) 344, 1459, 1517
BmgT120I GGNCC 3 cut(s) 1091, 1117, 1577
BmiI GGNNCC 2 cut(s) 609, 1129
BmrFI CCNGG 4 cut(s) 729, 1172, 1581, 1614
BmrI ACTGGG 1 cut(s) 721
BmsI GCATC 3 cut(s) 433, 820, 1630
BmuI ACTGGG 1 cut(s) 721
BpiI GAAGAC 1 cut(s) 1135
BpmI CTGGAG 2 cut(s) 284, 440
Bpu14I TTCGAA 1 cut(s) 658
BpuEI CTTGAG 1 cut(s) 635
BpuMI CCSGG 1 cut(s) 729
BsaHI GRCGYC 2 cut(s) 638, 1128
BsaI GGTCTC 1 cut(s) 286
BsaJI CCNNGG 3 cut(s) 1110, 1289, 1445
BsaWI WCCGGW 3 cut(s) 1119, 1358, 1712
Bsc4I CCNNNNNNNGG 3 cut(s) 1304, 1697, 1712
Bse118I RCCGGY 4 cut(s) 416, 723, 896, 1712
Bse1I ACTGG 4 cut(s) 292, 716, 988, 1505
Bse3DI GCAATG 3 cut(s) 461, 1000, 1258
BseAI TCCGGA 1 cut(s) 1119
BseBI CCWGG 3 cut(s) 1172, 1581, 1614
BseDI CCNNGG 3 cut(s) 1110, 1289, 1445
BseGI GGATG 4 cut(s) 20, 1009, 1368, 1531
BseLI CCNNNNNNNGG 3 cut(s) 1304, 1697, 1712
BseMI GCAATG 3 cut(s) 461, 1000, 1258
BseMII CTCAG 3 cut(s) 481, 1532, 1551
BseNI ACTGG 4 cut(s) 292, 716, 988, 1505
BseRI GAGGAG 1 cut(s) 248
BseX3I CGGCCG 1 cut(s) 725
BseXI GCAGC 8 cut(s) 736, 829, 1139, 1167, 1243, 1306, 1381, 1630
BsgI GTGCAG 2 cut(s) 1242, 1363
Bsh1236I CGCG 4 cut(s) 365, 425, 697, 1187
Bsh1285I CGRYCG 1 cut(s) 728
BshFI GGCC 6 cut(s) 727, 1109, 1166, 1308, 1444, 1612
BshNI GGYRCC 2 cut(s) 607, 1127
BshTI ACCGGT 1 cut(s) 1712
BsiEI CGRYCG 1 cut(s) 728
BsiHKAI GWGCWC 3 cut(s) 440, 540, 1547
BsiHKCI CYCGRG 3 cut(s) 344, 1459, 1517
BsiSI CCGG 9 cut(s) 149, 417, 724, 728, 753, 897, 1120, 1359, 1713
BslFI GGGAC 2 cut(s) 530, 649
BslI CCNNNNNNNGG 3 cut(s) 1304, 1697, 1712
BsmAI GTCTC 2 cut(s) 286, 583
BsmBI CGTCTC 1 cut(s) 583
BsmFI GGGAC 2 cut(s) 530, 649
BsmI GAATGC 2 cut(s) 1189, 1220
BsnI GGCC 6 cut(s) 727, 1109, 1166, 1308, 1444, 1612
Bso31I GGTCTC 1 cut(s) 286
BsoBI CYCGRG 3 cut(s) 344, 1459, 1517
Bsp119I TTCGAA 1 cut(s) 658
Bsp1286I GDGCHC 3 cut(s) 440, 540, 1547
Bsp13I TCCGGA 1 cut(s) 1119
Bsp1407I TGTACA 2 cut(s) 560, 570
Bsp143I GATC 6 cut(s) 451, 512, 951, 1007, 1501, 1583
Bsp19I CCATGG 1 cut(s) 1445
BspACI CCGC 9 cut(s) 146, 330, 425, 548, 736, 991, 1115, 1392, 1397
BspANI GGCC 6 cut(s) 727, 1109, 1166, 1308, 1444, 1612
BspCNI CTCAG 3 cut(s) 480, 1533, 1552
BspEI TCCGGA 1 cut(s) 1119
BspFNI CGCG 4 cut(s) 365, 425, 697, 1187
BspHI TCATGA 1 cut(s) 477
BspLI GGNNCC 2 cut(s) 609, 1129
BspMAI CTGCAG 1 cut(s) 1157
BspPI GGATC 3 cut(s) 1015, 1496, 1591
BspQI GCTCTTC 1 cut(s) 62
BspT104I TTCGAA 1 cut(s) 658
BspT107I GGYRCC 2 cut(s) 607, 1127
BspTNI GGTCTC 1 cut(s) 286
BsrDI GCAATG 3 cut(s) 461, 1000, 1258
BsrFI RCCGGY 4 cut(s) 416, 723, 896, 1712
BsrGI TGTACA 2 cut(s) 560, 570
BsrI ACTGG 4 cut(s) 292, 716, 988, 1505
BssAI RCCGGY 4 cut(s) 416, 723, 896, 1712
BssECI CCNNGG 3 cut(s) 1110, 1289, 1445
BssMI GATC 6 cut(s) 451, 512, 951, 1007, 1501, 1583
BssNI GRCGYC 2 cut(s) 638, 1128
BssT1I CCWWGG 1 cut(s) 1445
Bst2UI CCWGG 3 cut(s) 1172, 1581, 1614
Bst4CI ACNGT 9 cut(s) 311, 526, 560, 1075, 1234, 1277, 1423, 1468, 1657
Bst6I CTCTTC 3 cut(s) 62, 92, 903
BstACI GRCGYC 2 cut(s) 638, 1128
BstAPI GCANNNNNTGC 1 cut(s) 1640
BstAUI TGTACA 2 cut(s) 560, 570
BstBI TTCGAA 1 cut(s) 658
BstC8I GCNNGC 2 cut(s) 725, 1107
BstDEI CTNAG 6 cut(s) 51, 467, 541, 1541, 1560, 1720
BstDSI CCRYGG 2 cut(s) 1110, 1445
BstEII GGTNACC 1 cut(s) 1616
BstENI CCTNNNNNAGG 1 cut(s) 1695
BstF5I GGATG 4 cut(s) 20, 1009, 1368, 1531
BstFNI CGCG 4 cut(s) 365, 425, 697, 1187
BstH2I RGCGCY 1 cut(s) 1131
BstHHI GCGC 4 cut(s) 697, 847, 1130, 1189
BstKTI GATC 6 cut(s) 454, 515, 954, 1010, 1504, 1586
BstMAI GTCTC 2 cut(s) 286, 583
BstMBI GATC 6 cut(s) 451, 512, 951, 1007, 1501, 1583
BstMCI CGRYCG 1 cut(s) 728
BstMWI GCNNNNNNNGC 8 cut(s) 338, 573, 733, 839, 1034, 1043, 1403, 1640
BstNI CCWGG 3 cut(s) 1172, 1581, 1614
BstNSI RCATGY 1 cut(s) 571
BstPI GGTNACC 1 cut(s) 1616
BstSCI CCNGG 4 cut(s) 727, 1170, 1579, 1612
BstSFI CTRYAG 2 cut(s) 1147, 1153
BstUI CGCG 4 cut(s) 365, 425, 697, 1187
BstV1I GCAGC 8 cut(s) 736, 829, 1139, 1167, 1243, 1306, 1381, 1630
BstV2I GAAGAC 1 cut(s) 1135
BstXI CCANNNNNNTGG 3 cut(s) 40, 1512, 1669
BstZI CGGCCG 1 cut(s) 725
BsuRI GGCC 6 cut(s) 727, 1109, 1166, 1308, 1444, 1612
BtgI CCRYGG 2 cut(s) 1110, 1445
BtsCI GGATG 4 cut(s) 20, 1009, 1368, 1531
BtsI GCAGTG 1 cut(s) 891
BtsIMutI CAGTG 7 cut(s) 475, 674, 891, 981, 1419, 1538, 1662
Cac8I GCNNGC 2 cut(s) 725, 1107
CaiI CAGNNNCTG 1 cut(s) 1394
CciI TCATGA 1 cut(s) 477
CfoI GCGC 4 cut(s) 697, 847, 1130, 1189
Cfr10I RCCGGY 4 cut(s) 416, 723, 896, 1712
Cfr13I GGNCC 3 cut(s) 1091, 1117, 1577
CpoI CGGWCCG 1 cut(s) 1117
CseI GACGC 7 cut(s) 243, 354, 431, 512, 565, 646, 915
Csp6I GTAC 6 cut(s) 295, 561, 571, 706, 940, 1456
CspAI ACCGGT 1 cut(s) 1712
CspCI CAANNNNNGTGG 2 cut(s) 679, 714
CspI CGGWCCG 1 cut(s) 1117
CviAII CATG 8 cut(s) 40, 370, 397, 478, 568, 1446, 1486, 1608
CviQI GTAC 6 cut(s) 295, 561, 571, 706, 940, 1456
DdeI CTNAG 6 cut(s) 51, 467, 541, 1541, 1560, 1720
DinI GGCGCC 1 cut(s) 1129
DpnI GATC 6 cut(s) 453, 514, 953, 1009, 1503, 1585
DpnII GATC 6 cut(s) 451, 512, 951, 1007, 1501, 1583
DrdI GACNNNNNNGTC 1 cut(s) 1139
DseDI GACNNNNNNGTC 1 cut(s) 1139
EaeI YGGCCR 1 cut(s) 725
EagI CGGCCG 1 cut(s) 725
Eam1104I CTCTTC 3 cut(s) 62, 92, 903
EarI CTCTTC 3 cut(s) 62, 92, 903
Ecl136II GAGCTC 2 cut(s) 438, 538
EclXI CGGCCG 1 cut(s) 725
Eco130I CCWWGG 1 cut(s) 1445
Eco147I AGGCCT 1 cut(s) 1166
Eco24I GRGCYC 2 cut(s) 440, 540
Eco31I GGTCTC 1 cut(s) 286
Eco47I GGWCC 3 cut(s) 1091, 1117, 1577
Eco52I CGGCCG 1 cut(s) 725
Eco53kI GAGCTC 2 cut(s) 438, 538
Eco57I CTGAAG 1 cut(s) 728
Eco88I CYCGRG 3 cut(s) 344, 1459, 1517
Eco91I GGTNACC 1 cut(s) 1616
EcoICRI GAGCTC 2 cut(s) 438, 538
EcoNI CCTNNNNNAGG 1 cut(s) 1695
EcoO109I RGGNCCY 1 cut(s) 1577
EcoO65I GGTNACC 1 cut(s) 1616
EcoRII CCWGG 3 cut(s) 1170, 1579, 1612
EcoT14I CCWWGG 1 cut(s) 1445
EcoT38I GRGCYC 2 cut(s) 440, 540
EgeI GGCGCC 1 cut(s) 1129
EheI GGCGCC 1 cut(s) 1129
ErhI CCWWGG 1 cut(s) 1445
Esp3I CGTCTC 1 cut(s) 583
FaeI CATG 8 cut(s) 43, 373, 400, 481, 571, 1449, 1489, 1611
FalI AAGNNNNNCTT 2 cut(s) 926, 958
FaqI GGGAC 2 cut(s) 530, 649
FatI CATG 8 cut(s) 39, 369, 396, 477, 567, 1445, 1485, 1607
FauI CCCGC 2 cut(s) 153, 555
FblI GTMKAC 1 cut(s) 222
FokI GGATG 4 cut(s) 7, 1016, 1355, 1538
FriOI GRGCYC 2 cut(s) 440, 540
FspBI CTAG 4 cut(s) 864, 1034, 1436, 1593
GlaI GCGC 4 cut(s) 696, 846, 1129, 1188
GsuI CTGGAG 2 cut(s) 284, 440
HaeII RGCGCY 1 cut(s) 1131
HaeIII GGCC 6 cut(s) 727, 1109, 1166, 1308, 1444, 1612
HapII CCGG 9 cut(s) 149, 417, 724, 728, 753, 897, 1120, 1359, 1713
HgaI GACGC 7 cut(s) 243, 354, 431, 512, 565, 646, 915
HhaI GCGC 4 cut(s) 697, 847, 1130, 1189
Hin1I GRCGYC 2 cut(s) 638, 1128
Hin1II CATG 8 cut(s) 43, 373, 400, 481, 571, 1449, 1489, 1611
Hin6I GCGC 4 cut(s) 695, 845, 1128, 1187
HinP1I GCGC 4 cut(s) 695, 845, 1128, 1187
HincII GTYRAC 3 cut(s) 247, 409, 1066
HindII GTYRAC 3 cut(s) 247, 409, 1066
HinfI GANTC 3 cut(s) 1022, 1062, 1348
HpaI GTTAAC 1 cut(s) 409
HpaII CCGG 9 cut(s) 149, 417, 724, 728, 753, 897, 1120, 1359, 1713
HphI GGTGA 3 cut(s) 727, 802, 808
Hpy166II GTNNAC 8 cut(s) 223, 247, 409, 594, 706, 1066, 1237, 1471
Hpy188I TCNGA 9 cut(s) 233, 435, 442, 502, 512, 956, 972, 1199, 1676
Hpy188III TCNNGA 6 cut(s) 301, 457, 478, 1120, 1372, 1426
Hpy8I GTNNAC 8 cut(s) 223, 247, 409, 594, 706, 1066, 1237, 1471
Hpy99I CGWCG 3 cut(s) 125, 425, 931
HpyAV CCTTC 4 cut(s) 189, 726, 1043, 1444
HpyCH4III ACNGT 9 cut(s) 311, 526, 560, 1075, 1234, 1277, 1423, 1468, 1657
HpyCH4IV ACGT 2 cut(s) 123, 219
HpyF10VI GCNNNNNNNGC 8 cut(s) 338, 573, 733, 839, 1034, 1043, 1403, 1640
HpyF3I CTNAG 6 cut(s) 51, 467, 541, 1541, 1560, 1720
HpySE526I ACGT 2 cut(s) 123, 219
Hsp92I GRCGYC 2 cut(s) 638, 1128
Hsp92II CATG 8 cut(s) 43, 373, 400, 481, 571, 1449, 1489, 1611
HspAI GCGC 4 cut(s) 695, 845, 1128, 1187
KasI GGCGCC 1 cut(s) 1127
Kpn2I TCCGGA 1 cut(s) 1119
KroI GCCGGC 1 cut(s) 723
KroNI GCCGGC 1 cut(s) 725
KspAI GTTAAC 1 cut(s) 409
Kzo9I GATC 6 cut(s) 451, 512, 951, 1007, 1501, 1583
LguI GCTCTTC 1 cut(s) 62
LmnI GCTCC 1 cut(s) 443
Lsp1109I GCAGC 8 cut(s) 736, 829, 1139, 1167, 1243, 1306, 1381, 1630
LweI GCATC 3 cut(s) 433, 820, 1630
MaeI CTAG 4 cut(s) 864, 1034, 1436, 1593
MaeII ACGT 2 cut(s) 123, 219
MaeIII GTNAC 8 cut(s) 517, 617, 672, 889, 1277, 1412, 1603, 1616
MalI GATC 6 cut(s) 453, 514, 953, 1009, 1503, 1585
MboI GATC 6 cut(s) 451, 512, 951, 1007, 1501, 1583
MboII GAAGA 6 cut(s) 49, 79, 102, 734, 920, 1135
MhlI GDGCHC 3 cut(s) 440, 540, 1547
MluCI AATT 4 cut(s) 1202, 1480, 1648, 1681
Mly113I GGCGCC 1 cut(s) 1128
MlyI GAGTC 2 cut(s) 1016, 1071
MmeI TCCRAC 3 cut(s) 1222, 1654, 1686
MroI TCCGGA 1 cut(s) 1119
MroNI GCCGGC 1 cut(s) 723
MseI TTAA 3 cut(s) 408, 774, 966
MslI CAYNNNNRTG 3 cut(s) 38, 210, 785
MspA1I CMGCKG 2 cut(s) 1152, 1394
MspI CCGG 9 cut(s) 149, 417, 724, 728, 753, 897, 1120, 1359, 1713
MspR9I CCNGG 4 cut(s) 729, 1172, 1581, 1614
Mva1269I GAATGC 2 cut(s) 1189, 1220
MvaI CCWGG 3 cut(s) 1172, 1581, 1614
MvnI CGCG 4 cut(s) 365, 425, 697, 1187
MwoI GCNNNNNNNGC 8 cut(s) 338, 573, 733, 839, 1034, 1043, 1403, 1640
NaeI GCCGGC 1 cut(s) 725
NarI GGCGCC 1 cut(s) 1128
NciI CCSGG 1 cut(s) 729
NcoI CCATGG 1 cut(s) 1445
NdeII GATC 6 cut(s) 451, 512, 951, 1007, 1501, 1583
NgoMIV GCCGGC 1 cut(s) 723
NlaIII CATG 8 cut(s) 43, 373, 400, 481, 571, 1449, 1489, 1611
NlaIV GGNNCC 2 cut(s) 609, 1129
NmeAIII GCCGAG 1 cut(s) 1270
NmuCI GTSAC 4 cut(s) 517, 617, 672, 889
NspI RCATGY 1 cut(s) 571
NspV TTCGAA 1 cut(s) 658
PaeR7I CTCGAG 3 cut(s) 344, 1459, 1517
PagI TCATGA 1 cut(s) 477
PceI AGGCCT 1 cut(s) 1166
PciSI GCTCTTC 1 cut(s) 62
PcsI WCGNNNNNNNCGW 1 cut(s) 926
PctI GAATGC 2 cut(s) 1189, 1220
PdiI GCCGGC 1 cut(s) 725
PfeI GAWTC 1 cut(s) 1348
PinAI ACCGGT 1 cut(s) 1712
PleI GAGTC 2 cut(s) 1016, 1070
PluTI GGCGCC 1 cut(s) 1131
PpsI GAGTC 2 cut(s) 1016, 1070
PpuMI RGGWCCY 1 cut(s) 1577
PshBI ATTAAT 1 cut(s) 966
Psp124BI GAGCTC 2 cut(s) 440, 540
Psp5II RGGWCCY 1 cut(s) 1577
Psp6I CCWGG 3 cut(s) 1170, 1579, 1612
PspEI GGTNACC 1 cut(s) 1616
PspGI CCWGG 3 cut(s) 1170, 1579, 1612
PspN4I GGNNCC 2 cut(s) 609, 1129
PspPI GGNCC 3 cut(s) 1091, 1117, 1577
PspPPI RGGWCCY 1 cut(s) 1577
PspXI VCTCGAGB 3 cut(s) 344, 1459, 1517
PsrI GAACNNNNNNTAC 2 cut(s) 923, 955
PstI CTGCAG 1 cut(s) 1157
PstNI CAGNNNCTG 1 cut(s) 1394
PvuII CAGCTG 1 cut(s) 1152
RsaI GTAC 6 cut(s) 296, 562, 572, 707, 941, 1457
RsaNI GTAC 6 cut(s) 295, 561, 571, 706, 940, 1456
RseI CAYNNNNRTG 3 cut(s) 38, 210, 785
Rsr2I CGGWCCG 1 cut(s) 1117
RsrII CGGWCCG 1 cut(s) 1117
SacI GAGCTC 2 cut(s) 440, 540
SapI GCTCTTC 1 cut(s) 62
SaqAI TTAA 3 cut(s) 408, 774, 966
Sau3AI GATC 6 cut(s) 451, 512, 951, 1007, 1501, 1583
Sau96I GGNCC 3 cut(s) 1091, 1117, 1577
ScaI AGTACT 2 cut(s) 941, 1457
SchI GAGTC 2 cut(s) 1016, 1071
ScrFI CCNGG 4 cut(s) 729, 1172, 1581, 1614
SduI GDGCHC 3 cut(s) 440, 540, 1547
SfaNI GCATC 3 cut(s) 433, 820, 1630
SfcI CTRYAG 2 cut(s) 1147, 1153
SfoI GGCGCC 1 cut(s) 1129
Sfr274I CTCGAG 3 cut(s) 344, 1459, 1517
SfuI TTCGAA 1 cut(s) 658
SgrAI CRCCGGYG 1 cut(s) 416
SinI GGWCC 3 cut(s) 1091, 1117, 1577
SlaI CTCGAG 3 cut(s) 344, 1459, 1517
SmiMI CAYNNNNRTG 3 cut(s) 38, 210, 785
SmlI CTYRAG 4 cut(s) 344, 650, 1459, 1517
SmoI CTYRAG 4 cut(s) 344, 650, 1459, 1517
SpeI ACTAGT 1 cut(s) 1592
Sse9I AATT 4 cut(s) 1202, 1480, 1648, 1681
SseBI AGGCCT 1 cut(s) 1166
SsiI CCGC 9 cut(s) 146, 330, 425, 548, 736, 991, 1115, 1392, 1397
SspDI GGCGCC 1 cut(s) 1127
SspMI CTAG 4 cut(s) 864, 1034, 1436, 1593
SstI GAGCTC 2 cut(s) 440, 540
StuI AGGCCT 1 cut(s) 1166
StyD4I CCNGG 4 cut(s) 727, 1170, 1579, 1612
StyI CCWWGG 1 cut(s) 1445
TaaI ACNGT 9 cut(s) 311, 526, 560, 1075, 1234, 1277, 1423, 1468, 1657
TaiI ACGT 2 cut(s) 126, 222
TaqII GACCGA 1 cut(s) 1129
TasI AATT 4 cut(s) 1202, 1480, 1648, 1681
TatI WGTACW 4 cut(s) 560, 570, 939, 1455
TauI GCSGC 2 cut(s) 739, 1394
TfiI GAWTC 1 cut(s) 1348
Tru1I TTAA 3 cut(s) 408, 774, 966
Tru9I TTAA 3 cut(s) 408, 774, 966
TscAI CASTG 7 cut(s) 475, 681, 898, 988, 1426, 1545, 1662
TseFI GTSAC 4 cut(s) 517, 617, 672, 889
TseI GCWGC 8 cut(s) 749, 842, 1152, 1155, 1256, 1294, 1394, 1643
Tsp45I GTSAC 4 cut(s) 517, 617, 672, 889
TspDTI ATGAA 6 cut(s) 102, 373, 386, 921, 1231, 1474
TspGWI ACGGA 2 cut(s) 184, 232
TspRI CASTG 7 cut(s) 475, 681, 898, 988, 1426, 1545, 1662
VpaK11BI GGWCC 3 cut(s) 1091, 1117, 1577
VspI ATTAAT 1 cut(s) 966
XagI CCTNNNNNAGG 1 cut(s) 1695
XapI RAATTY 2 cut(s) 1480, 1648
XceI RCATGY 1 cut(s) 571
XhoI CTCGAG 3 cut(s) 344, 1459, 1517
XmiI GTMKAC 1 cut(s) 222
XspI CTAG 4 cut(s) 864, 1034, 1436, 1593
ZrmI AGTACT 2 cut(s) 941, 1457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.