Rh6BG362800

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000522
Physical Location & Seq
Reverse (-)
59285716 .. 59287575
1860 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG362800.1

Sequence Viewer

Length: 1470 bp
ATGCTACCCAACCAAACAAACAATGTGTATGACTACGGCCGTTACGCGGTCCGGAAATCGTTATCACAATCCCGTAAATTACTTAACTTGGTGAACAAGTATCTCCAACGCAACTCGACTCTCTCCAAAACCGCAATCCTCGCCCTTGAAGATTGCAAGCTTCTTGCAGAACTCAACGTAGATTTTCTTGCAAGCTCCCTACAAACCCTCAACGACACTAACCGAACACTTCCCAGCTCGAAAGCCGAGGACGTCCATACCTGGCTCAGTGCCGTCCTAACAAACCAGCAAACATGCTTGGATGGCGTACAAGCAACTTCTTCTTCATCATCTTGGAGGAGTATGAAAAATGGACTTTTGCTCTCCAACAACACGAACCTTTATAGTGTTTCTCTAGCTCTGTTCACGAAGGGATGGGTGCCTGAAAATGAGGAAGACGAGGAGAAGGAGGAGGAGGGGGAGGTGATCACATGGAGGAAACGGCTGCTTCAGAATTTTGGTGACGTGCTGGTGAGGGAGACTGTCACTGTGAGTCAGGACGGAAGTGGGAACTTCACCACCATAACCGACGCTGTTGCCGCAGCTCCAGTTAACACTAAAGGTTTAAATGGTTACTTCTTGATTTATATCAAGGCAGGAGTTTATGAAGAGTATGTGAACATTGCAAATACGAAAAACTATCTGATGATGATTGGTGAGGGCATCAACCAGACTGTCATAACAGGCAATCGGAGTGTTGTAGATGGCTGGACAACTTTCAATTCAGCAACATTTGTGGTAATTGCACCATGGTTTGTAGCAGTGAACATAACCTTCCGCAACACCGCCGGAGCAATCAAACACCAAGCCGTCGCCGTCCGAAACGGTGGAGATTTGTCCGCATTCTATAATTGCAGCTTCGAAGGGTATCAAGACACCTTATACACACATTCCCAAAGACAATTCTACAGAGACTGCAACGTCTACGGAACAGTGGACTTCATATTTGGAAACGCAGCAGCTGTTTTCCAAAAATGTAACATATACCCCCGGCTTCCGATGCGTGGGCAGTTCAACGCCATTACGGCTCAGGGTCGAGCAGACCCGAATCAAAACACCGGCATTATGATCCACAACTGTACCATCAGAGCTTCCGATGACTTGGCTTCGGACAAGGGAACTACCAGGACTTATCTAGGGAGGCCATGGAAGGAGTATTCAAGGACGGTGTACATGAAATCCTTCATGGATGGTTGCATAGATAGGGAAGGTTGGAGTAAATGGACTGGAGATTTTGCTTTAAGCACATTGTATTACGCCGAGTATAAATGTCAGGGCCCTGGATCGAACACTAGTAGAAGAGTTACATGGCCAGGTTACCATATATTGAAAAAAGCTAAGCAAGCTAAAAAGTTTACAGTGTCCAGGTTCTTGCTTGGGAAGAAGTGGCTGCCTCAAACTGGAGTGCCATTTTCCGGTGGGTTGATTTAG

Protein Analysis

489

Amino Acids

54.76

Weight (kDa)

9.03

Isoelectric Point (pI)

31.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 12 - 134 3.8e-19 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 175 - 473 1.8e-136 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000522)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02810 AT2G47550 AT4G02330
fragaria_vesca FvH4_2g25960 FvH4_2g25960 FvH4_2g25970 FvH4_5g35570 FvH4_5g35580 FvH4_5g35590
malus_domestica MD03G1290900.v1.1 MD03G1291000.v1.1 MD08G1195600.v1.1 MD11G1307400.v1.1 MD11G1307500.v1.1
prunus_persica Prupe.1G529400_v2.0.a1 Prupe.1G529500_v2.0.a1 Prupe.8G263900_v2.0.a1 Prupe.8G264000_v2.0.a1
pyrus_communis pycom08g16800
rosa_chinensis RchiOBHm_Chr6g0294321 RchiOBHm_Chr6g0294331 RchiOBHm_Chr7g0236951 RchiOBHm_Chr7g0236971 RchiOBHm_Chr7g0237041 RchiOBHm_Chr7g0237051
rosa_laevigata RLG00000001034 RLG00000001035 RLG00000001039 RLG00000001043 RLG00000001045 RLG00000011880 RLG00000011881 RLG00000034181
rosa_multiflora Rmu_co8086188.1_g000001 Rmu_co8458485.1_g000001 Rmu_sc0000272.1_g000007 Rmu_sc0000272.1_g000009 Rmu_sc0002131.1_g000030 Rmu_sc0002131.1_g000037 Rmu_sc0005369.1_g000005 Rmu_sc0005369.1_g000008 Rmu_sc0007991.1_g000005 Rmu_sc0007991.1_g000013 Rmu_sc0033563.1_g000001
rosa_roxburghii Rroxscaffold_3G00224540 Rroxscaffold_3G00224600 Rroxscaffold_3G00224650 Rroxscaffold_3G00224660 Rroxscaffold_7G00173470 Rroxscaffold_7G00173480
rosa_rugosa Rorug06G0243400 Rorug06G0243500 Rorug07G0301200 Rorug07G0301500 Rorug07G0301600
rosa_samantha Rh6AG356000 Rh6AG356100 Rh6BG362600 Rh6BG362800 Rh6CG370000 Rh6CG370100 Rh6DG356500 Rh6DG356600 Rh7AG456200 Rh7AG456500 Rh7AG457500 Rh7AG457600 Rh7BG427500 Rh7BG427700 Rh7BG428400 Rh7BG428500 Rh7CG475100 Rh7CG475300 Rh7CG475700 Rh7CG475800 Rh7DG444100 Rh7DG444300 Rh7DG444700 Rh7DG444900
rosa_wichuraiana Rw6G031020 Rw6G031030 Rw7G037870 Rw7G037880 Rw7G037920 Rw7G037940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 959
AatII GACGTC 1 cut(s) 255
AccB1I GGYRCC 1 cut(s) 418
AccI GTMKAC 1 cut(s) 963
AccII CGCG 1 cut(s) 47
AccIII TCCGGA 1 cut(s) 51
AciI CCGC 6 cut(s) 47, 132, 579, 817, 825, 879
AclWI GGATC 2 cut(s) 1102, 1330
AcoI YGGCCR 2 cut(s) 37, 1349
AcsI RAATTY 1 cut(s) 493
AcuI CTGAAG 1 cut(s) 473
AcyI GRCGYC 1 cut(s) 252
AfaI GTAC 3 cut(s) 309, 1120, 1211
AfiI CCNNNNNNNGG 4 cut(s) 46, 1043, 1439, 1454
AgsI TTSAA 5 cut(s) 149, 760, 1054, 1200, 1369
AhlI ACTAGT 1 cut(s) 1331
AjiI CACGTC 1 cut(s) 505
AjnI CCWGG 5 cut(s) 260, 1163, 1318, 1351, 1403
Alw26I GTCTC 2 cut(s) 512, 945
AlwI GGATC 2 cut(s) 1102, 1330
AlwNI CAGNNNCTG 2 cut(s) 954, 1001
Aor13HI TCCGGA 1 cut(s) 51
AoxI GGCC 4 cut(s) 37, 1181, 1315, 1349
ApaI GGGCCC 1 cut(s) 1319
ApeKI GCWGC 6 cut(s) 484, 581, 894, 995, 998, 1429
ApoI RAATTY 1 cut(s) 493
ArsI GACNNNNNNTTYG 2 cut(s) 968, 1000
Asp700I GAANNNNTTC 1 cut(s) 1220
AspS9I GGNCC 3 cut(s) 49, 1315, 1316
AsuC2I CCSGG 1 cut(s) 1030
AsuHPI GGTGA 6 cut(s) 103, 475, 512, 523, 547, 707
AsuII TTCGAA 1 cut(s) 900
AvaII GGWCC 1 cut(s) 49
BaeGI GKGCMC 1 cut(s) 1319
BaeI ACNNNNGTAYC 2 cut(s) 1102, 1135
BalI TGGCCA 1 cut(s) 1351
BanI GGYRCC 1 cut(s) 418
BanII GRGCYC 1 cut(s) 1319
BbsI GAAGAC 1 cut(s) 441
BbvI GCAGC 6 cut(s) 471, 593, 906, 1007, 1010, 1416
BccI CCATC 5 cut(s) 296, 408, 737, 1130, 1223
BceAI ACGGC 7 cut(s) 24, 52, 257, 497, 833, 839, 1080
BcgI CGANNNNNNTGC 2 cut(s) 557, 591
BciT130I CCWGG 5 cut(s) 262, 1165, 1320, 1353, 1405
BclI TGATCA 1 cut(s) 465
BcnI CCSGG 1 cut(s) 1030
BcoDI GTCTC 2 cut(s) 512, 945
BcuI ACTAGT 1 cut(s) 1331
BfaI CTAG 3 cut(s) 395, 1175, 1332
BfmI CTRYAG 1 cut(s) 946
BglI GCCNNNNNGGC 1 cut(s) 1064
BisI GCNGC 7 cut(s) 485, 579, 582, 895, 996, 999, 1430
BlpI GCTNAGC 1 cut(s) 1377
BlsI GCNGC 7 cut(s) 486, 580, 583, 896, 997, 1000, 1431
Bme1390I CCNGG 6 cut(s) 262, 1030, 1165, 1320, 1353, 1405
Bme18I GGWCC 1 cut(s) 49
BmgBI CACGTC 1 cut(s) 505
BmgT120I GGNCC 3 cut(s) 49, 1315, 1316
BmiI GGNNCC 2 cut(s) 420, 1317
BmrFI CCNGG 6 cut(s) 262, 1030, 1165, 1320, 1353, 1405
BmsI GCATC 2 cut(s) 711, 1029
BpiI GAAGAC 1 cut(s) 441
BpmI CTGGAG 3 cut(s) 570, 1287, 1461
Bpu10I CCTNAGC 1 cut(s) 1068
Bpu1102I GCTNAGC 1 cut(s) 1377
Bpu14I TTCGAA 1 cut(s) 900
BpuMI CCSGG 1 cut(s) 1030
BsaBI GATNNNNATC 1 cut(s) 626
BsaHI GRCGYC 1 cut(s) 252
BsaJI CCNNGG 5 cut(s) 246, 788, 1028, 1184, 1318
BsaWI WCCGGW 2 cut(s) 51, 1454
Bsc4I CCNNNNNNNGG 4 cut(s) 46, 1043, 1439, 1454
Bse118I RCCGGY 1 cut(s) 1097
Bse1I ACTGG 3 cut(s) 587, 1270, 1444
Bse3DI GCAATG 1 cut(s) 660
Bse8I GATNNNNATC 1 cut(s) 626
BseAI TCCGGA 1 cut(s) 51
BseBI CCWGG 5 cut(s) 262, 1165, 1320, 1353, 1405
BseDI CCNNGG 5 cut(s) 246, 788, 1028, 1184, 1318
BseGI GGATG 3 cut(s) 307, 419, 1234
BseJI GATNNNNATC 1 cut(s) 626
BseLI CCNNNNNNNGG 4 cut(s) 46, 1043, 1439, 1454
BseMI GCAATG 1 cut(s) 660
BseMII CTCAG 2 cut(s) 280, 1082
BseNI ACTGG 3 cut(s) 587, 1270, 1444
BseRI GAGGAG 4 cut(s) 352, 455, 464, 467
BseSI GKGCMC 1 cut(s) 1319
BseX3I CGGCCG 1 cut(s) 37
BseXI GCAGC 6 cut(s) 471, 593, 906, 1007, 1010, 1416
BseYI CCCAGC 1 cut(s) 233
Bsh1236I CGCG 1 cut(s) 47
Bsh1285I CGRYCG 1 cut(s) 40
BshFI GGCC 4 cut(s) 39, 1183, 1317, 1351
BshNI GGYRCC 1 cut(s) 418
BsiEI CGRYCG 1 cut(s) 40
BsiSI CCGG 5 cut(s) 52, 828, 1030, 1098, 1455
BslI CCNNNNNNNGG 4 cut(s) 46, 1043, 1439, 1454
BsmAI GTCTC 2 cut(s) 512, 945
BsmI GAATGC 1 cut(s) 881
BsnI GGCC 4 cut(s) 39, 1183, 1317, 1351
Bsp119I TTCGAA 1 cut(s) 900
Bsp120I GGGCCC 1 cut(s) 1315
Bsp1286I GDGCHC 1 cut(s) 1319
Bsp13I TCCGGA 1 cut(s) 51
Bsp1407I TGTACA 1 cut(s) 1209
Bsp143I GATC 3 cut(s) 465, 1107, 1322
Bsp1720I GCTNAGC 1 cut(s) 1377
Bsp19I CCATGG 2 cut(s) 788, 1184
BspACI CCGC 6 cut(s) 47, 132, 579, 817, 825, 879
BspANI GGCC 4 cut(s) 39, 1183, 1317, 1351
BspCNI CTCAG 2 cut(s) 279, 1081
BspEI TCCGGA 1 cut(s) 51
BspFNI CGCG 1 cut(s) 47
BspLI GGNNCC 2 cut(s) 420, 1317
BspPI GGATC 2 cut(s) 1102, 1330
BspT104I TTCGAA 1 cut(s) 900
BspT107I GGYRCC 1 cut(s) 418
BsrDI GCAATG 1 cut(s) 660
BsrFI RCCGGY 1 cut(s) 1097
BsrGI TGTACA 1 cut(s) 1209
BsrI ACTGG 3 cut(s) 587, 1270, 1444
BssAI RCCGGY 1 cut(s) 1097
BssECI CCNNGG 5 cut(s) 246, 788, 1028, 1184, 1318
BssMI GATC 3 cut(s) 465, 1107, 1322
BssNI GRCGYC 1 cut(s) 252
BssT1I CCWWGG 2 cut(s) 788, 1184
Bst2UI CCWGG 5 cut(s) 262, 1165, 1320, 1353, 1405
Bst4CI ACNGT 8 cut(s) 523, 529, 715, 866, 973, 1118, 1207, 1399
Bst6I CTCTTC 2 cut(s) 642, 1333
BstACI GRCGYC 1 cut(s) 252
BstAUI TGTACA 1 cut(s) 1209
BstBI TTCGAA 1 cut(s) 900
BstC8I GCNNGC 3 cut(s) 158, 193, 1383
BstDEI CTNAG 3 cut(s) 266, 1068, 1377
BstDSI CCRYGG 2 cut(s) 788, 1184
BstEII GGTNACC 1 cut(s) 1355
BstF5I GGATG 3 cut(s) 307, 419, 1234
BstFNI CGCG 1 cut(s) 47
BstKTI GATC 3 cut(s) 468, 1110, 1325
BstMAI GTCTC 2 cut(s) 512, 945
BstMBI GATC 3 cut(s) 465, 1107, 1322
BstMCI CGRYCG 1 cut(s) 40
BstMWI GCNNNNNNNGC 6 cut(s) 140, 303, 578, 1039, 1064, 1382
BstNI CCWGG 5 cut(s) 262, 1165, 1320, 1353, 1405
BstNSI RCATGY 1 cut(s) 297
BstPI GGTNACC 1 cut(s) 1355
BstSCI CCNGG 6 cut(s) 260, 1028, 1163, 1318, 1351, 1403
BstSFI CTRYAG 1 cut(s) 946
BstSLI GKGCMC 1 cut(s) 1319
BstUI CGCG 1 cut(s) 47
BstV1I GCAGC 6 cut(s) 471, 593, 906, 1007, 1010, 1416
BstV2I GAAGAC 1 cut(s) 441
BstZI CGGCCG 1 cut(s) 37
BsuRI GGCC 4 cut(s) 39, 1183, 1317, 1351
BtgI CCRYGG 2 cut(s) 788, 1184
BtrI CACGTC 1 cut(s) 505
BtsCI GGATG 3 cut(s) 307, 419, 1234
BtsI GCAGTG 1 cut(s) 807
BtsIMutI CAGTG 5 cut(s) 274, 525, 807, 978, 1404
Cac8I GCNNGC 3 cut(s) 158, 193, 1383
CaiI CAGNNNCTG 2 cut(s) 954, 1001
Cfr10I RCCGGY 1 cut(s) 1097
Cfr13I GGNCC 3 cut(s) 49, 1315, 1316
CpoI CGGWCCG 1 cut(s) 49
CseI GACGC 1 cut(s) 578
Csp6I GTAC 3 cut(s) 308, 1119, 1210
CspCI CAANNNNNGTGG 2 cut(s) 756, 791
CspI CGGWCCG 1 cut(s) 49
CviAII CATG 7 cut(s) 294, 471, 789, 1185, 1213, 1225, 1347
CviQI GTAC 3 cut(s) 308, 1119, 1210
DdeI CTNAG 3 cut(s) 266, 1068, 1377
DpnI GATC 3 cut(s) 467, 1109, 1324
DpnII GATC 3 cut(s) 465, 1107, 1322
DraI TTTAAA 1 cut(s) 606
DrdI GACNNNNNNGTC 1 cut(s) 959
DseDI GACNNNNNNGTC 1 cut(s) 959
EaeI YGGCCR 2 cut(s) 37, 1349
EagI CGGCCG 1 cut(s) 37
Eam1104I CTCTTC 2 cut(s) 642, 1333
EarI CTCTTC 2 cut(s) 642, 1333
EclXI CGGCCG 1 cut(s) 37
Eco130I CCWWGG 2 cut(s) 788, 1184
Eco24I GRGCYC 1 cut(s) 1319
Eco47I GGWCC 1 cut(s) 49
Eco52I CGGCCG 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 473
Eco91I GGTNACC 1 cut(s) 1355
EcoO109I RGGNCCY 2 cut(s) 1315, 1316
EcoO65I GGTNACC 1 cut(s) 1355
EcoRII CCWGG 5 cut(s) 260, 1163, 1318, 1351, 1403
EcoT14I CCWWGG 2 cut(s) 788, 1184
EcoT38I GRGCYC 1 cut(s) 1319
ErhI CCWWGG 2 cut(s) 788, 1184
FaeI CATG 7 cut(s) 297, 474, 792, 1188, 1216, 1228, 1350
FatI CATG 7 cut(s) 293, 470, 788, 1184, 1212, 1224, 1346
FbaI TGATCA 1 cut(s) 465
FblI GTMKAC 1 cut(s) 963
Fnu4HI GCNGC 7 cut(s) 485, 579, 582, 895, 996, 999, 1430
FokI GGATG 3 cut(s) 314, 426, 1241
FriOI GRGCYC 1 cut(s) 1319
Fsp4HI GCNGC 7 cut(s) 485, 579, 582, 895, 996, 999, 1430
FspBI CTAG 3 cut(s) 395, 1175, 1332
GluI GCNGC 7 cut(s) 485, 579, 582, 895, 996, 999, 1430
GsaI CCCAGC 1 cut(s) 237
GsuI CTGGAG 3 cut(s) 570, 1287, 1461
HaeIII GGCC 4 cut(s) 39, 1183, 1317, 1351
HapII CCGG 5 cut(s) 52, 828, 1030, 1098, 1455
HgaI GACGC 1 cut(s) 578
Hin1I GRCGYC 1 cut(s) 252
Hin1II CATG 7 cut(s) 297, 474, 792, 1188, 1216, 1228, 1350
HincII GTYRAC 1 cut(s) 592
HindII GTYRAC 1 cut(s) 592
HindIII AAGCTT 1 cut(s) 158
HinfI GANTC 3 cut(s) 118, 532, 1087
HpaI GTTAAC 1 cut(s) 592
HpaII CCGG 5 cut(s) 52, 828, 1030, 1098, 1455
HphI GGTGA 6 cut(s) 103, 475, 512, 523, 547, 707
Hpy166II GTNNAC 9 cut(s) 94, 405, 592, 658, 805, 964, 976, 1210, 1395
Hpy188I TCNGA 8 cut(s) 492, 684, 732, 860, 1038, 1127, 1135, 1150
Hpy188III TCNNGA 5 cut(s) 52, 406, 536, 619, 911
Hpy8I GTNNAC 9 cut(s) 94, 405, 592, 658, 805, 964, 976, 1210, 1395
Hpy99I CGWCG 2 cut(s) 572, 854
HpyAV CCTTC 7 cut(s) 403, 439, 823, 896, 1183, 1231, 1241
HpyCH4III ACNGT 8 cut(s) 523, 529, 715, 866, 973, 1118, 1207, 1399
HpyCH4IV ACGT 4 cut(s) 177, 252, 504, 960
HpyCH4V TGCA 8 cut(s) 156, 167, 191, 665, 785, 894, 957, 1236
HpyF10VI GCNNNNNNNGC 6 cut(s) 140, 303, 578, 1039, 1064, 1382
HpyF3I CTNAG 3 cut(s) 266, 1068, 1377
HpySE526I ACGT 4 cut(s) 177, 252, 504, 960
Hsp92I GRCGYC 1 cut(s) 252
Hsp92II CATG 7 cut(s) 297, 474, 792, 1188, 1216, 1228, 1350
Kpn2I TCCGGA 1 cut(s) 51
Ksp22I TGATCA 1 cut(s) 465
KspAI GTTAAC 1 cut(s) 592
Kzo9I GATC 3 cut(s) 465, 1107, 1322
LmnI GCTCC 3 cut(s) 200, 589, 830
Lsp1109I GCAGC 6 cut(s) 471, 593, 906, 1007, 1010, 1416
LweI GCATC 2 cut(s) 711, 1029
MaeI CTAG 3 cut(s) 395, 1175, 1332
MaeII ACGT 4 cut(s) 177, 252, 504, 960
MaeIII GTNAC 7 cut(s) 41, 500, 523, 611, 1016, 1342, 1355
MalI GATC 3 cut(s) 467, 1109, 1324
MboI GATC 3 cut(s) 465, 1107, 1322
MboII GAAGA 7 cut(s) 161, 312, 315, 446, 659, 1350, 1432
MhlI GDGCHC 1 cut(s) 1319
MlsI TGGCCA 1 cut(s) 1351
MluCI AATT 6 cut(s) 77, 493, 760, 780, 889, 941
MluNI TGGCCA 1 cut(s) 1351
MlyI GAGTC 2 cut(s) 112, 541
MmeI TCCRAC 3 cut(s) 130, 390, 1232
Mox20I TGGCCA 1 cut(s) 1351
MroI TCCGGA 1 cut(s) 51
MroXI GAANNNNTTC 1 cut(s) 1220
MscI TGGCCA 1 cut(s) 1351
MseI TTAA 4 cut(s) 84, 591, 605, 1280
Msp20I TGGCCA 1 cut(s) 1351
MspA1I CMGCKG 1 cut(s) 1001
MspI CCGG 5 cut(s) 52, 828, 1030, 1098, 1455
MspR9I CCNGG 6 cut(s) 262, 1030, 1165, 1320, 1353, 1405
Mva1269I GAATGC 1 cut(s) 881
MvaI CCWGG 5 cut(s) 262, 1165, 1320, 1353, 1405
MvnI CGCG 1 cut(s) 47
MwoI GCNNNNNNNGC 6 cut(s) 140, 303, 578, 1039, 1064, 1382
NciI CCSGG 1 cut(s) 1030
NcoI CCATGG 2 cut(s) 788, 1184
NdeII GATC 3 cut(s) 465, 1107, 1322
NlaIII CATG 7 cut(s) 297, 474, 792, 1188, 1216, 1228, 1350
NlaIV GGNNCC 2 cut(s) 420, 1317
NmeAIII GCCGAG 2 cut(s) 271, 1324
NmuCI GTSAC 2 cut(s) 500, 523
NspI RCATGY 1 cut(s) 297
NspV TTCGAA 1 cut(s) 900
PctI GAATGC 1 cut(s) 881
PdmI GAANNNNTTC 1 cut(s) 1220
PfeI GAWTC 1 cut(s) 1087
PkrI GCNGC 7 cut(s) 486, 580, 583, 896, 997, 1000, 1431
PleI GAGTC 2 cut(s) 112, 540
PpsI GAGTC 2 cut(s) 112, 540
Psp6I CCWGG 5 cut(s) 260, 1163, 1318, 1351, 1403
PspEI GGTNACC 1 cut(s) 1355
PspFI CCCAGC 1 cut(s) 233
PspGI CCWGG 5 cut(s) 260, 1163, 1318, 1351, 1403
PspN4I GGNNCC 2 cut(s) 420, 1317
PspOMI GGGCCC 1 cut(s) 1315
PspPI GGNCC 3 cut(s) 49, 1315, 1316
PstNI CAGNNNCTG 2 cut(s) 954, 1001
PvuII CAGCTG 1 cut(s) 1001
RsaI GTAC 3 cut(s) 309, 1120, 1211
RsaNI GTAC 3 cut(s) 308, 1119, 1210
Rsr2I CGGWCCG 1 cut(s) 49
RsrII CGGWCCG 1 cut(s) 49
SaqAI TTAA 4 cut(s) 84, 591, 605, 1280
SatI GCNGC 7 cut(s) 485, 579, 582, 895, 996, 999, 1430
Sau3AI GATC 3 cut(s) 465, 1107, 1322
Sau96I GGNCC 3 cut(s) 49, 1315, 1316
SchI GAGTC 2 cut(s) 112, 541
ScrFI CCNGG 6 cut(s) 262, 1030, 1165, 1320, 1353, 1405
SduI GDGCHC 1 cut(s) 1319
SfaNI GCATC 2 cut(s) 711, 1029
SfcI CTRYAG 1 cut(s) 946
SfuI TTCGAA 1 cut(s) 900
SinI GGWCC 1 cut(s) 49
SpeI ACTAGT 1 cut(s) 1331
Sse9I AATT 6 cut(s) 77, 493, 760, 780, 889, 941
SsiI CCGC 6 cut(s) 47, 132, 579, 817, 825, 879
SspMI CTAG 3 cut(s) 395, 1175, 1332
StyD4I CCNGG 6 cut(s) 260, 1028, 1163, 1318, 1351, 1403
StyI CCWWGG 2 cut(s) 788, 1184
TaaI ACNGT 8 cut(s) 523, 529, 715, 866, 973, 1118, 1207, 1399
TaiI ACGT 4 cut(s) 180, 255, 507, 963
TaqI TCGA 5 cut(s) 116, 239, 900, 1075, 1325
TasI AATT 6 cut(s) 77, 493, 760, 780, 889, 941
TatI WGTACW 1 cut(s) 1209
TauI GCSGC 1 cut(s) 581
TfiI GAWTC 1 cut(s) 1087
Tru1I TTAA 4 cut(s) 84, 591, 605, 1280
Tru9I TTAA 4 cut(s) 84, 591, 605, 1280
TscAI CASTG 5 cut(s) 274, 532, 807, 978, 1404
TseFI GTSAC 2 cut(s) 500, 523
TseI GCWGC 6 cut(s) 484, 581, 894, 995, 998, 1429
Tsp45I GTSAC 2 cut(s) 500, 523
TspDTI ATGAA 6 cut(s) 315, 359, 660, 970, 1213, 1229
TspGWI ACGGA 2 cut(s) 555, 981
TspRI CASTG 5 cut(s) 274, 532, 807, 978, 1404
VpaK11BI GGWCC 1 cut(s) 49
XapI RAATTY 1 cut(s) 493
XceI RCATGY 1 cut(s) 297
XmiI GTMKAC 1 cut(s) 963
XmnI GAANNNNTTC 1 cut(s) 1220
XspI CTAG 3 cut(s) 395, 1175, 1332
ZraI GACGTC 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.