Rroxscaffold_3G00224540

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000522
Physical Location & Seq
Reverse (-)
7740401 .. 7742378
1978 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00224540.1

Sequence Viewer

Length: 1689 bp
ATGCCTTATCTCTTCAATCCTCTGTCATCATCACTATTCCTCATTCTCTTCTCTTTGCTCATCTCTCAATCTTCATCTGCCGACGTTCCTCTTAACACTTCCCTCCCGCCGGAAACCATCTGCAACTCCACCCCACACCCTTCTTACTGCTTGTCCGTCCTCCCTCACAAAAATGCCAATGTCTACGAGTTCGGCCGCTTCTCCGTCCAACACTCTCTCCTCCAATCCCAAAACCTAATGGTCTCCCTCAACCAATACCTCCAAAGTTACCGTTCTGCTTTATCGCAAACAGCCATTCAAGCCCTCGAGGATTGCAAGTTCCTCGCGTCCCTGAACATCGACTTCATCTCAAACACCATAAAAAAAGTTAACAGCAGCAGCGACGCCGTGCTTCCGAGCTCGGCAGCAGATGATCTCCAGACATTGCTCAGCGCCATCATGACAAACCAGCAGACTTGTTCAGACGCACTCCAATCTGTCACTACTGTCAAAGACGAGCTCTTAGTCCCGCTCTTAAACTGTACAAGTATGTACAGCGTCTCTTTGGCTCTGTTCACAGAGGGTTGGGTGCCAAAGGATCACAAAAATGCTAACGGGAAGCCGAACCATCTTGGTTTTCGAAAGGGTAGGTTGTCACTGAAAATGTCGAGCAAAGCTCGTGGGGTCTACGAATGGGTGATAAGTCGGCCAGGAAGGCGGCTTCTTCAGGCGGCTGGAGATGAGGAAGTTGTGGTTAGGGACATTGTGGTGGTGAGCCAGGATGGAAGTGGGAACTTCACCACCATCAATGATGCTATTAATGCAGCGCCGAACAACTCTGTTGCTAGTGATGGGTATTTCTTGGTGTATGTCACCGCCGGTGTTTATGAAGAGTATGTTTCGATTGCGTCGAACAAGCAGTACTTGTTGCTGATCGGAGACGGTATCAATCAGACCATCATCACCGGGAACCGCAATGTCGTAGATGGATCGACTACTTTCGACTCTGCTACTTTAGCTGTGGTGGCAGAAGGGTTTGTGGGAGTCAACATAACCATTCGTAACACGGCTGGACCAAGTAAAATGCAGGCTGTGGCGGTCAGAAACGGCGCGGACCTGTCAGTCTTCTACAGATGCAGCTTTGAAGCCTACCAGGACACCCTGTACGCGCATTCTCTCCGACAATTCTACCGAGAATGTGACATCTATGGAACTGTAGATTTCATATTCGGCAATGCTGCAGTTGTTTTACAAAACTGTAACCTCTACCCTCGCCAGCCACTCAAAGGCCAATCCAATCCCATCACAGCTCAGGGCCGAACTGACCCGAATCAGAATACCGGCACTTCCATCCAGAACTGCACCATTACAGCCGCTGCCGACTTGGCTTCGAGTAACTTCACTGTCAAGACATATCTAGGGAGGCCATGGAAGGAGTATTCGAGGACGGTTTACTTGCAGAATTTCATGGATAGTTTGATTGATCCTGTTGGTTGGCTCGAGTGGAATGGAGATTTTGCACTGAGCACATTGTATTATGCTGAATATGATAACAGAGGACCTGGATCAGACACTAGTAATAGAGTCACATGGCCTGGTTACCATGTGATCAATGCAACTGATGCTGTTAATTTTACAGTGTCTAACTTTCTGGTAGGGGATAGCTGGTTGCCTCAGACAGGTGTGCCTTATACTGGTGGCTTTGTTTGA

Protein Analysis

562

Amino Acids

61.53

Weight (kDa)

5.34

Isoelectric Point (pI)

36.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 38 - 184 1.2e-21 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 249 - 547 1.1e-138 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000522)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02810 AT2G47550 AT4G02330
fragaria_vesca FvH4_2g25960 FvH4_2g25960 FvH4_2g25970 FvH4_5g35570 FvH4_5g35580 FvH4_5g35590
malus_domestica MD03G1290900.v1.1 MD03G1291000.v1.1 MD08G1195600.v1.1 MD11G1307400.v1.1 MD11G1307500.v1.1
prunus_persica Prupe.1G529400_v2.0.a1 Prupe.1G529500_v2.0.a1 Prupe.8G263900_v2.0.a1 Prupe.8G264000_v2.0.a1
pyrus_communis pycom08g16800
rosa_chinensis RchiOBHm_Chr6g0294321 RchiOBHm_Chr6g0294331 RchiOBHm_Chr7g0236951 RchiOBHm_Chr7g0236971 RchiOBHm_Chr7g0237041 RchiOBHm_Chr7g0237051
rosa_laevigata RLG00000001034 RLG00000001035 RLG00000001039 RLG00000001043 RLG00000001045 RLG00000011880 RLG00000011881 RLG00000034181
rosa_multiflora Rmu_co8086188.1_g000001 Rmu_co8458485.1_g000001 Rmu_sc0000272.1_g000007 Rmu_sc0000272.1_g000009 Rmu_sc0002131.1_g000030 Rmu_sc0002131.1_g000037 Rmu_sc0005369.1_g000005 Rmu_sc0005369.1_g000008 Rmu_sc0007991.1_g000005 Rmu_sc0007991.1_g000013 Rmu_sc0033563.1_g000001
rosa_roxburghii Rroxscaffold_3G00224540 Rroxscaffold_3G00224600 Rroxscaffold_3G00224650 Rroxscaffold_3G00224660 Rroxscaffold_7G00173470 Rroxscaffold_7G00173480
rosa_rugosa Rorug06G0243400 Rorug06G0243500 Rorug07G0301200 Rorug07G0301500 Rorug07G0301600
rosa_samantha Rh6AG356000 Rh6AG356100 Rh6BG362600 Rh6BG362800 Rh6CG370000 Rh6CG370100 Rh6DG356500 Rh6DG356600 Rh7AG456200 Rh7AG456500 Rh7AG457500 Rh7AG457600 Rh7BG427500 Rh7BG427700 Rh7BG428400 Rh7BG428500 Rh7CG475100 Rh7CG475300 Rh7CG475700 Rh7CG475800 Rh7DG444100 Rh7DG444300 Rh7DG444700 Rh7DG444900
rosa_wichuraiana Rw6G031020 Rw6G031030 Rw7G037870 Rw7G037880 Rw7G037920 Rw7G037940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1100
AbsI CCTCGAGG 1 cut(s) 305
AccB1I GGYRCC 1 cut(s) 568
AccBSI CCGCTC 1 cut(s) 511
AccI GTMKAC 2 cut(s) 183, 666
AccII CGCG 3 cut(s) 326, 1091, 1148
AclWI GGATC 4 cut(s) 585, 976, 1457, 1552
AcoI YGGCCR 2 cut(s) 193, 686
AcsI RAATTY 1 cut(s) 1441
AcuI CTGAAG 1 cut(s) 689
AcyI GRCGYC 1 cut(s) 384
AfaI GTAC 4 cut(s) 523, 533, 902, 1145
AfiI CCNNNNNNNGG 4 cut(s) 109, 1265, 1658, 1673
AgsI TTSAA 3 cut(s) 16, 299, 1124
AhlI ACTAGT 1 cut(s) 1553
AjnI CCWGG 5 cut(s) 688, 756, 1131, 1540, 1573
AloI GAACNNNNNNTCC 2 cut(s) 302, 334
AluBI AGCT 7 cut(s) 399, 499, 656, 998, 1119, 1289, 1644
AluI AGCT 7 cut(s) 399, 499, 656, 998, 1119, 1289, 1644
Alw21I GWGCWC 3 cut(s) 401, 501, 1508
Alw26I GTCTC 3 cut(s) 247, 544, 912
AlwI GGATC 4 cut(s) 585, 976, 1457, 1552
AlwNI CAGNNNCTG 1 cut(s) 1355
Ama87I CYCGRG 2 cut(s) 305, 1478
AoxI GGCC 6 cut(s) 193, 686, 1267, 1294, 1403, 1571
ApeKI GCWGC 7 cut(s) 375, 378, 404, 803, 1116, 1217, 1355
ApoI RAATTY 1 cut(s) 1441
AseI ATTAAT 1 cut(s) 798
AspLEI GCGC 4 cut(s) 434, 808, 1091, 1150
AspS9I GGNCC 4 cut(s) 1052, 1093, 1294, 1538
AsuC2I CCSGG 1 cut(s) 946
AsuHPI GGTGA 5 cut(s) 688, 763, 769, 844, 934
AsuII TTCGAA 1 cut(s) 619
AvaI CYCGRG 2 cut(s) 305, 1478
AvaII GGWCC 3 cut(s) 1052, 1093, 1538
BanI GGYRCC 1 cut(s) 568
BanII GRGCYC 2 cut(s) 401, 501
BauI CACGAG 1 cut(s) 657
BbsI GAAGAC 1 cut(s) 1096
Bbv12I GWGCWC 3 cut(s) 401, 501, 1508
BbvI GCAGC 7 cut(s) 387, 390, 416, 815, 1128, 1204, 1342
BceAI ACGGC 3 cut(s) 371, 1062, 1102
BcgI CGANNNNNNTGC 2 cut(s) 304, 338
BciT130I CCWGG 5 cut(s) 690, 758, 1133, 1542, 1575
BclI TGATCA 1 cut(s) 1587
BcnI CCSGG 1 cut(s) 946
BcoDI GTCTC 3 cut(s) 247, 544, 912
BcuI ACTAGT 1 cut(s) 1553
BfaI CTAG 3 cut(s) 825, 1397, 1554
BfmI CTRYAG 3 cut(s) 1108, 1194, 1218
BfoI RGCGCY 2 cut(s) 435, 809
BglI GCCNNNNNGGC 2 cut(s) 694, 1364
BlpI GCTNAGC 1 cut(s) 428
BmcAI AGTACT 1 cut(s) 902
Bme1390I CCNGG 6 cut(s) 690, 758, 946, 1133, 1542, 1575
Bme18I GGWCC 3 cut(s) 1052, 1093, 1538
BmeT110I CYCGRG 2 cut(s) 305, 1478
BmgT120I GGNCC 4 cut(s) 1052, 1093, 1294, 1538
BmiI GGNNCC 2 cut(s) 570, 950
BmrFI CCNGG 6 cut(s) 690, 758, 946, 1133, 1542, 1575
BmsI GCATC 3 cut(s) 781, 1103, 1591
BpiI GAAGAC 1 cut(s) 1096
BplI GAGNNNNNCTC 2 cut(s) 640, 672
BpmI CTGGAG 2 cut(s) 401, 735
Bpu10I CCTNAGC 1 cut(s) 1290
Bpu1102I GCTNAGC 1 cut(s) 428
Bpu14I TTCGAA 1 cut(s) 619
BpuMI CCSGG 1 cut(s) 946
BsaHI GRCGYC 1 cut(s) 384
BsaI GGTCTC 1 cut(s) 247
BsaJI CCNNGG 1 cut(s) 1406
BsaXI ACNNNNNCTCC 2 cut(s) 201, 231
Bsc4I CCNNNNNNNGG 4 cut(s) 109, 1265, 1658, 1673
Bse118I RCCGGY 2 cut(s) 857, 1319
Bse1I ACTGG 1 cut(s) 1678
Bse3DI GCAATG 3 cut(s) 422, 961, 1219
BseBI CCWGG 5 cut(s) 690, 758, 1133, 1542, 1575
BseDI CCNNGG 1 cut(s) 1406
BseGI GGATG 2 cut(s) 766, 1329
BseLI CCNNNNNNNGG 4 cut(s) 109, 1265, 1658, 1673
BseMI GCAATG 3 cut(s) 422, 961, 1219
BseMII CTCAG 4 cut(s) 442, 1304, 1493, 1667
BseNI ACTGG 1 cut(s) 1678
BseRI GAGGAG 1 cut(s) 209
BseX3I CGGCCG 1 cut(s) 193
BseXI GCAGC 7 cut(s) 387, 390, 416, 815, 1128, 1204, 1342
BsgI GTGCAG 1 cut(s) 1324
Bsh1236I CGCG 3 cut(s) 326, 1091, 1148
Bsh1285I CGRYCG 1 cut(s) 196
BshFI GGCC 6 cut(s) 195, 688, 1269, 1296, 1405, 1573
BshNI GGYRCC 1 cut(s) 568
BsiEI CGRYCG 1 cut(s) 196
BsiHKAI GWGCWC 3 cut(s) 401, 501, 1508
BsiHKCI CYCGRG 2 cut(s) 305, 1478
BsiSI CCGG 4 cut(s) 110, 858, 945, 1320
BslFI GGGAC 3 cut(s) 313, 491, 752
BslI CCNNNNNNNGG 4 cut(s) 109, 1265, 1658, 1673
BsmAI GTCTC 3 cut(s) 247, 544, 912
BsmBI CGTCTC 2 cut(s) 544, 912
BsmFI GGGAC 3 cut(s) 313, 491, 752
BsmI GAATGC 1 cut(s) 1150
BsnI GGCC 6 cut(s) 195, 688, 1269, 1296, 1405, 1573
Bso31I GGTCTC 1 cut(s) 247
BsoBI CYCGRG 2 cut(s) 305, 1478
Bsp119I TTCGAA 1 cut(s) 619
Bsp1286I GDGCHC 3 cut(s) 401, 501, 1508
Bsp1407I TGTACA 2 cut(s) 521, 531
Bsp143I GATC 7 cut(s) 412, 577, 912, 968, 1462, 1544, 1587
Bsp1720I GCTNAGC 1 cut(s) 428
Bsp19I CCATGG 1 cut(s) 1406
BspANI GGCC 6 cut(s) 195, 688, 1269, 1296, 1405, 1573
BspCNI CTCAG 4 cut(s) 441, 1303, 1494, 1666
BspFNI CGCG 3 cut(s) 326, 1091, 1148
BspHI TCATGA 1 cut(s) 438
BspLI GGNNCC 2 cut(s) 570, 950
BspMAI CTGCAG 1 cut(s) 1222
BspPI GGATC 4 cut(s) 585, 976, 1457, 1552
BspT104I TTCGAA 1 cut(s) 619
BspT107I GGYRCC 1 cut(s) 568
BspTNI GGTCTC 1 cut(s) 247
BsrBI CCGCTC 1 cut(s) 511
BsrDI GCAATG 3 cut(s) 422, 961, 1219
BsrFI RCCGGY 2 cut(s) 857, 1319
BsrGI TGTACA 2 cut(s) 521, 531
BsrI ACTGG 1 cut(s) 1678
BssAI RCCGGY 2 cut(s) 857, 1319
BssECI CCNNGG 1 cut(s) 1406
BssMI GATC 7 cut(s) 412, 577, 912, 968, 1462, 1544, 1587
BssNI GRCGYC 1 cut(s) 384
BssSI CACGAG 1 cut(s) 657
BssT1I CCWWGG 1 cut(s) 1406
Bst2BI CACGAG 1 cut(s) 657
Bst2UI CCWGG 5 cut(s) 690, 758, 1133, 1542, 1575
Bst4CI ACNGT 9 cut(s) 272, 487, 521, 923, 1195, 1238, 1384, 1429, 1618
Bst6I CTCTTC 3 cut(s) 17, 53, 864
BstACI GRCGYC 1 cut(s) 384
BstAPI GCANNNNNTGC 1 cut(s) 1601
BstAUI TGTACA 2 cut(s) 521, 531
BstBI TTCGAA 1 cut(s) 619
BstC8I GCNNGC 2 cut(s) 1068, 1256
BstDEI CTNAG 5 cut(s) 428, 502, 1290, 1502, 1653
BstDSI CCRYGG 1 cut(s) 1406
BstEII GGTNACC 1 cut(s) 1577
BstENI CCTNNNNNAGG 1 cut(s) 1656
BstF5I GGATG 2 cut(s) 766, 1329
BstFNI CGCG 3 cut(s) 326, 1091, 1148
BstH2I RGCGCY 2 cut(s) 435, 809
BstHHI GCGC 4 cut(s) 434, 808, 1091, 1150
BstKTI GATC 7 cut(s) 415, 580, 915, 971, 1465, 1547, 1590
BstMAI GTCTC 3 cut(s) 247, 544, 912
BstMBI GATC 7 cut(s) 412, 577, 912, 968, 1462, 1544, 1587
BstMCI CGRYCG 1 cut(s) 196
BstMWI GCNNNNNNNGC 7 cut(s) 299, 694, 800, 995, 1004, 1364, 1601
BstNI CCWGG 5 cut(s) 690, 758, 1133, 1542, 1575
BstPI GGTNACC 1 cut(s) 1577
BstSCI CCNGG 6 cut(s) 688, 756, 944, 1131, 1540, 1573
BstSFI CTRYAG 3 cut(s) 1108, 1194, 1218
BstUI CGCG 3 cut(s) 326, 1091, 1148
BstV1I GCAGC 7 cut(s) 387, 390, 416, 815, 1128, 1204, 1342
BstV2I GAAGAC 1 cut(s) 1096
BstZI CGGCCG 1 cut(s) 193
BsuRI GGCC 6 cut(s) 195, 688, 1269, 1296, 1405, 1573
BtgI CCRYGG 1 cut(s) 1406
BtsCI GGATG 2 cut(s) 766, 1329
BtsIMutI CAGTG 4 cut(s) 635, 1380, 1499, 1623
Cac8I GCNNGC 2 cut(s) 1068, 1256
CaiI CAGNNNCTG 1 cut(s) 1355
CciI TCATGA 1 cut(s) 438
CfoI GCGC 4 cut(s) 434, 808, 1091, 1150
Cfr10I RCCGGY 2 cut(s) 857, 1319
Cfr13I GGNCC 4 cut(s) 1052, 1093, 1294, 1538
CseI GACGC 5 cut(s) 315, 392, 473, 526, 876
Csp6I GTAC 4 cut(s) 522, 532, 901, 1144
CspCI CAANNNNNGTGG 2 cut(s) 640, 675
CviAII CATG 5 cut(s) 439, 1407, 1447, 1569, 1583
CviQI GTAC 4 cut(s) 522, 532, 901, 1144
DdeI CTNAG 5 cut(s) 428, 502, 1290, 1502, 1653
DpnI GATC 7 cut(s) 414, 579, 914, 970, 1464, 1546, 1589
DpnII GATC 7 cut(s) 412, 577, 912, 968, 1462, 1544, 1587
DrdI GACNNNNNNGTC 1 cut(s) 1100
DseDI GACNNNNNNGTC 1 cut(s) 1100
EaeI YGGCCR 2 cut(s) 193, 686
EagI CGGCCG 1 cut(s) 193
Eam1104I CTCTTC 3 cut(s) 17, 53, 864
EarI CTCTTC 3 cut(s) 17, 53, 864
Ecl136II GAGCTC 2 cut(s) 399, 499
EclXI CGGCCG 1 cut(s) 193
Eco130I CCWWGG 1 cut(s) 1406
Eco24I GRGCYC 2 cut(s) 401, 501
Eco31I GGTCTC 1 cut(s) 247
Eco47I GGWCC 3 cut(s) 1052, 1093, 1538
Eco52I CGGCCG 1 cut(s) 193
Eco53kI GAGCTC 2 cut(s) 399, 499
Eco57I CTGAAG 1 cut(s) 689
Eco88I CYCGRG 2 cut(s) 305, 1478
Eco91I GGTNACC 1 cut(s) 1577
EcoICRI GAGCTC 2 cut(s) 399, 499
EcoNI CCTNNNNNAGG 1 cut(s) 1656
EcoO109I RGGNCCY 1 cut(s) 1538
EcoO65I GGTNACC 1 cut(s) 1577
EcoRII CCWGG 5 cut(s) 688, 756, 1131, 1540, 1573
EcoT14I CCWWGG 1 cut(s) 1406
EcoT38I GRGCYC 2 cut(s) 401, 501
ErhI CCWWGG 1 cut(s) 1406
Esp3I CGTCTC 2 cut(s) 544, 912
FaeI CATG 5 cut(s) 442, 1410, 1450, 1572, 1586
FalI AAGNNNNNCTT 2 cut(s) 887, 919
FaqI GGGAC 3 cut(s) 313, 491, 752
FatI CATG 5 cut(s) 438, 1406, 1446, 1568, 1582
FauI CCCGC 2 cut(s) 114, 516
FbaI TGATCA 1 cut(s) 1587
FblI GTMKAC 2 cut(s) 183, 666
FokI GGATG 2 cut(s) 773, 1316
FriOI GRGCYC 2 cut(s) 401, 501
FspBI CTAG 3 cut(s) 825, 1397, 1554
GlaI GCGC 4 cut(s) 433, 807, 1090, 1149
GsuI CTGGAG 2 cut(s) 401, 735
HaeII RGCGCY 2 cut(s) 435, 809
HaeIII GGCC 6 cut(s) 195, 688, 1269, 1296, 1405, 1573
HapII CCGG 4 cut(s) 110, 858, 945, 1320
HgaI GACGC 5 cut(s) 315, 392, 473, 526, 876
HhaI GCGC 4 cut(s) 434, 808, 1091, 1150
Hin1I GRCGYC 1 cut(s) 384
Hin1II CATG 5 cut(s) 442, 1410, 1450, 1572, 1586
Hin6I GCGC 4 cut(s) 432, 806, 1089, 1148
HinP1I GCGC 4 cut(s) 432, 806, 1089, 1148
HincII GTYRAC 2 cut(s) 370, 1027
HindII GTYRAC 2 cut(s) 370, 1027
HinfI GANTC 4 cut(s) 983, 1023, 1309, 1563
HpaI GTTAAC 1 cut(s) 370
HpaII CCGG 4 cut(s) 110, 858, 945, 1320
HphI GGTGA 5 cut(s) 688, 763, 769, 844, 934
Hpy166II GTNNAC 6 cut(s) 184, 370, 555, 667, 1027, 1432
Hpy188I TCNGA 9 cut(s) 396, 463, 917, 933, 1082, 1160, 1314, 1549, 1656
Hpy188III TCNNGA 4 cut(s) 418, 439, 1333, 1387
Hpy8I GTNNAC 6 cut(s) 184, 370, 555, 667, 1027, 1432
Hpy99I CGWCG 3 cut(s) 86, 386, 892
HpyAV CCTTC 4 cut(s) 150, 687, 1004, 1405
HpyCH4III ACNGT 9 cut(s) 272, 487, 521, 923, 1195, 1238, 1384, 1429, 1618
HpyCH4IV ACGT 1 cut(s) 84
HpyF10VI GCNNNNNNNGC 7 cut(s) 299, 694, 800, 995, 1004, 1364, 1601
HpyF3I CTNAG 5 cut(s) 428, 502, 1290, 1502, 1653
HpySE526I ACGT 1 cut(s) 84
Hsp92I GRCGYC 1 cut(s) 384
Hsp92II CATG 5 cut(s) 442, 1410, 1450, 1572, 1586
HspAI GCGC 4 cut(s) 432, 806, 1089, 1148
Ksp22I TGATCA 1 cut(s) 1587
KspAI GTTAAC 1 cut(s) 370
Kzo9I GATC 7 cut(s) 412, 577, 912, 968, 1462, 1544, 1587
Lsp1109I GCAGC 7 cut(s) 387, 390, 416, 815, 1128, 1204, 1342
LweI GCATC 3 cut(s) 781, 1103, 1591
MaeI CTAG 3 cut(s) 825, 1397, 1554
MaeII ACGT 1 cut(s) 84
MalI GATC 7 cut(s) 414, 579, 914, 970, 1464, 1546, 1589
MbiI CCGCTC 1 cut(s) 511
MboI GATC 7 cut(s) 412, 577, 912, 968, 1462, 1544, 1587
MboII GAAGA 6 cut(s) 4, 40, 63, 695, 881, 1096
MhlI GDGCHC 3 cut(s) 401, 501, 1508
MluCI AATT 3 cut(s) 1163, 1441, 1609
MlyI GAGTC 3 cut(s) 977, 1032, 1572
MmeI TCCRAC 2 cut(s) 232, 1183
MseI TTAA 5 cut(s) 93, 369, 515, 798, 1608
MslI CAYNNNNRTG 3 cut(s) 171, 585, 746
MspA1I CMGCKG 1 cut(s) 1355
MspI CCGG 4 cut(s) 110, 858, 945, 1320
MspR9I CCNGG 6 cut(s) 690, 758, 946, 1133, 1542, 1575
Mva1269I GAATGC 1 cut(s) 1150
MvaI CCWGG 5 cut(s) 690, 758, 1133, 1542, 1575
MvnI CGCG 3 cut(s) 326, 1091, 1148
MwoI GCNNNNNNNGC 7 cut(s) 299, 694, 800, 995, 1004, 1364, 1601
NciI CCSGG 1 cut(s) 946
NcoI CCATGG 1 cut(s) 1406
NdeII GATC 7 cut(s) 412, 577, 912, 968, 1462, 1544, 1587
NlaIII CATG 5 cut(s) 442, 1410, 1450, 1572, 1586
NlaIV GGNNCC 2 cut(s) 570, 950
NmeAIII GCCGAG 1 cut(s) 380
NmuCI GTSAC 5 cut(s) 478, 633, 850, 1178, 1564
NspV TTCGAA 1 cut(s) 619
PaeR7I CTCGAG 2 cut(s) 305, 1478
PagI TCATGA 1 cut(s) 438
PcsI WCGNNNNNNNCGW 1 cut(s) 887
PctI GAATGC 1 cut(s) 1150
PfeI GAWTC 1 cut(s) 1309
PleI GAGTC 3 cut(s) 977, 1031, 1571
PpsI GAGTC 3 cut(s) 977, 1031, 1571
PpuMI RGGWCCY 1 cut(s) 1538
PshBI ATTAAT 1 cut(s) 798
Psp124BI GAGCTC 2 cut(s) 401, 501
Psp5II RGGWCCY 1 cut(s) 1538
Psp6I CCWGG 5 cut(s) 688, 756, 1131, 1540, 1573
PspEI GGTNACC 1 cut(s) 1577
PspGI CCWGG 5 cut(s) 688, 756, 1131, 1540, 1573
PspN4I GGNNCC 2 cut(s) 570, 950
PspPI GGNCC 4 cut(s) 1052, 1093, 1294, 1538
PspPPI RGGWCCY 1 cut(s) 1538
PspXI VCTCGAGB 2 cut(s) 305, 1478
PsrI GAACNNNNNNTAC 2 cut(s) 884, 916
PstI CTGCAG 1 cut(s) 1222
PstNI CAGNNNCTG 1 cut(s) 1355
RsaI GTAC 4 cut(s) 523, 533, 902, 1145
RsaNI GTAC 4 cut(s) 522, 532, 901, 1144
RseI CAYNNNNRTG 3 cut(s) 171, 585, 746
SacI GAGCTC 2 cut(s) 401, 501
SaqAI TTAA 5 cut(s) 93, 369, 515, 798, 1608
Sau3AI GATC 7 cut(s) 412, 577, 912, 968, 1462, 1544, 1587
Sau96I GGNCC 4 cut(s) 1052, 1093, 1294, 1538
ScaI AGTACT 1 cut(s) 902
SchI GAGTC 3 cut(s) 977, 1032, 1572
ScrFI CCNGG 6 cut(s) 690, 758, 946, 1133, 1542, 1575
SduI GDGCHC 3 cut(s) 401, 501, 1508
SfaNI GCATC 3 cut(s) 781, 1103, 1591
SfcI CTRYAG 3 cut(s) 1108, 1194, 1218
Sfr274I CTCGAG 2 cut(s) 305, 1478
SfuI TTCGAA 1 cut(s) 619
SgrAI CRCCGGYG 1 cut(s) 857
SinI GGWCC 3 cut(s) 1052, 1093, 1538
SlaI CTCGAG 2 cut(s) 305, 1478
SmiMI CAYNNNNRTG 3 cut(s) 171, 585, 746
SmlI CTYRAG 2 cut(s) 305, 1478
SmoI CTYRAG 2 cut(s) 305, 1478
SpeI ACTAGT 1 cut(s) 1553
Sse9I AATT 3 cut(s) 1163, 1441, 1609
SspMI CTAG 3 cut(s) 825, 1397, 1554
SstI GAGCTC 2 cut(s) 401, 501
StyD4I CCNGG 6 cut(s) 688, 756, 944, 1131, 1540, 1573
StyI CCWWGG 1 cut(s) 1406
TaaI ACNGT 9 cut(s) 272, 487, 521, 923, 1195, 1238, 1384, 1429, 1618
TaiI ACGT 1 cut(s) 87
TasI AATT 3 cut(s) 1163, 1441, 1609
TatI WGTACW 3 cut(s) 521, 531, 900
TauI GCSGC 4 cut(s) 198, 700, 713, 1355
TfiI GAWTC 1 cut(s) 1309
Tru1I TTAA 5 cut(s) 93, 369, 515, 798, 1608
Tru9I TTAA 5 cut(s) 93, 369, 515, 798, 1608
TscAI CASTG 4 cut(s) 642, 1387, 1506, 1623
TseFI GTSAC 5 cut(s) 478, 633, 850, 1178, 1564
TseI GCWGC 7 cut(s) 375, 378, 404, 803, 1116, 1217, 1355
Tsp45I GTSAC 5 cut(s) 478, 633, 850, 1178, 1564
TspDTI ATGAA 5 cut(s) 63, 334, 882, 1192, 1435
TspGWI ACGGA 2 cut(s) 145, 193
TspRI CASTG 4 cut(s) 642, 1387, 1506, 1623
VpaK11BI GGWCC 3 cut(s) 1052, 1093, 1538
VspI ATTAAT 1 cut(s) 798
XagI CCTNNNNNAGG 1 cut(s) 1656
XapI RAATTY 1 cut(s) 1441
XcmI CCANNNNNNNNNTGG 1 cut(s) 764
XhoI CTCGAG 2 cut(s) 305, 1478
XmiI GTMKAC 2 cut(s) 183, 666
XspI CTAG 3 cut(s) 825, 1397, 1554
ZrmI AGTACT 1 cut(s) 902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.