Rroxscaffold_7G00173470

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000522
Physical Location & Seq
Forward (+)
13463220 .. 13465210
1991 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00173470.1

Sequence Viewer

Length: 1638 bp
ATGGGTTCACAACCATTCTCTGTGTTCAAACTCTCGACTCTCATTCTTCATCTTATTCCCATCCTTTTTGCCTCTCGATCTTTAGCTACAGAGGCAGGTTGTGAAACTGATCCAGTATCCAAAGAAATCATTTGCGAATTCACCCCAAACCCTTCATATTGCAAGTCCATGCTACCCAACCAAACAAACAATGTGTATGACTACGGCCGTTACGCGGTCCGGAAATCATTATCACAATCCCATAAATTGCTTAACTTGGTGAACAAGTATCTCCAACGCAACTCTACTCTCTCCAAAACCGCAATCCTCACCCTTGAAGATTGCAAGCTTCTTGCAGAACTCAACGTAGATTTTCTTGCAAGCTCCCTACAAACCCTCAACGACACTAACCGAACACTTCCCAGCGCGAAAGCCGAGGACGTCCATACCTGGCTCAGTGCCGTCCTAACAAACCAGCAAACATGCTTGGATGGCGTACAAGCAACTTCTTCTTCATCATCTTGGAGGAGTATGAAAAATGGACTTTTGCACTCCAACAACACGAACCTTTATAGTGTTTCTCTAGCTCTGTTCACGAAGGGATGGGTGCCTGAAAATGAGGAAGAGGAGGAGGAGGAGGAGGAGGGGGAGGTGATCACATGGAGGAAACGGCTGCTTCAGAATTTTGGTGACGTGCTGGTGAGGGAGACTGTCACTGTGAGTCAGGACGGAAGTGGGAACTTCACCACCATAACCGACGCTGTTGCCGCAGCTCCGGTTAACACTAAAGGTTTAAATGGTTACTTCTTGATTTATATCAAGGCAGGAGTTTATGAAGAGTATGTGAACATTGCAAATACGAAAAACTATCTGATGATGATTGGTGAGGGCATCAACCAGACTGTCATAACAGGCAATCGGAGTGTTGTAGATGGCTGGACAACTTTCAATTCAGCAACATTTGTGGTAATTGCACCATGGTTTGTAGCAGTGAACATAACCTTCCGCAACACCGCCGGAGCAATCAAACACCAAGCCGTCGCCGTCCGAAACGGTGGAGATTTGTCCGCATTCTATAATTGCAGCTTCGAAGGATACCAAGACACCTTATACACACATTCCCAAAGACAATTCTACAGAGACTGCAACGTCTACGGAACAGTAGACTTCATATTTGGAAACGCAGCAGCTGTTTTCCAAAAATGTAACATTTACCCCCGGCTTCCGATACGTGGGCAGTTCAACGCCATTACGGCTCAGGGTCGAGCAGACCCGAATCAAAACACCGGCATTATGATCCACAACTGTACCATCAGAGCTTCCGATGACTTGGCTTCGGACAAGGGAACTACCAGGACTTATCTAGGGAGGCCATGGAAGGAGTATTCAAGGACTGTGTACATGAAATCCTTCATGGATGGTTGCATAGATAGGGAAGGTTGGAGTAAATGGACTGGAGATTTTGCTTTAAGCACATTGTATTACGCCGAGTATAAATGTCAGGGCCCCGGATCGAACACTAGTAGAAGAGTTACATGGCCAGGTTACCATATATTGAAAAAAGCTAAGCAAGCTAAAAAGTTTACAGTGTCCAAGTTCTTGCTTGGGAAGAAGTGGCTGCCTCAAACTGGAGTGCCATTTTCCGGTGGGTTGATTTAG

Protein Analysis

545

Amino Acids

60.85

Weight (kDa)

8.58

Isoelectric Point (pI)

34.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 44 - 190 8.1e-23 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 231 - 529 8.2e-136 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000522)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02810 AT2G47550 AT4G02330
fragaria_vesca FvH4_2g25960 FvH4_2g25960 FvH4_2g25970 FvH4_5g35570 FvH4_5g35580 FvH4_5g35590
malus_domestica MD03G1290900.v1.1 MD03G1291000.v1.1 MD08G1195600.v1.1 MD11G1307400.v1.1 MD11G1307500.v1.1
prunus_persica Prupe.1G529400_v2.0.a1 Prupe.1G529500_v2.0.a1 Prupe.8G263900_v2.0.a1 Prupe.8G264000_v2.0.a1
pyrus_communis pycom08g16800
rosa_chinensis RchiOBHm_Chr6g0294321 RchiOBHm_Chr6g0294331 RchiOBHm_Chr7g0236951 RchiOBHm_Chr7g0236971 RchiOBHm_Chr7g0237041 RchiOBHm_Chr7g0237051
rosa_laevigata RLG00000001034 RLG00000001035 RLG00000001039 RLG00000001043 RLG00000001045 RLG00000011880 RLG00000011881 RLG00000034181
rosa_multiflora Rmu_co8086188.1_g000001 Rmu_co8458485.1_g000001 Rmu_sc0000272.1_g000007 Rmu_sc0000272.1_g000009 Rmu_sc0002131.1_g000030 Rmu_sc0002131.1_g000037 Rmu_sc0005369.1_g000005 Rmu_sc0005369.1_g000008 Rmu_sc0007991.1_g000005 Rmu_sc0007991.1_g000013 Rmu_sc0033563.1_g000001
rosa_roxburghii Rroxscaffold_3G00224540 Rroxscaffold_3G00224600 Rroxscaffold_3G00224650 Rroxscaffold_3G00224660 Rroxscaffold_7G00173470 Rroxscaffold_7G00173480
rosa_rugosa Rorug06G0243400 Rorug06G0243500 Rorug07G0301200 Rorug07G0301500 Rorug07G0301600
rosa_samantha Rh6AG356000 Rh6AG356100 Rh6BG362600 Rh6BG362800 Rh6CG370000 Rh6CG370100 Rh6DG356500 Rh6DG356600 Rh7AG456200 Rh7AG456500 Rh7AG457500 Rh7AG457600 Rh7BG427500 Rh7BG427700 Rh7BG428400 Rh7BG428500 Rh7CG475100 Rh7CG475300 Rh7CG475700 Rh7CG475800 Rh7DG444100 Rh7DG444300 Rh7DG444700 Rh7DG444900
rosa_wichuraiana Rw6G031020 Rw6G031030 Rw7G037870 Rw7G037880 Rw7G037920 Rw7G037940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1127
AatII GACGTC 1 cut(s) 423
Acc36I ACCTGC 1 cut(s) 86
AccB1I GGYRCC 1 cut(s) 586
AccI GTMKAC 2 cut(s) 1131, 1143
AccII CGCG 2 cut(s) 215, 407
AccIII TCCGGA 1 cut(s) 219
AciI CCGC 6 cut(s) 215, 300, 747, 985, 993, 1047
AclWI GGATC 3 cut(s) 104, 1270, 1498
AcoI YGGCCR 2 cut(s) 205, 1517
AcsI RAATTY 2 cut(s) 137, 661
AcuI CTGAAG 1 cut(s) 641
AcyI GRCGYC 1 cut(s) 420
AfaI GTAC 3 cut(s) 477, 1288, 1379
AfiI CCNNNNNNNGG 4 cut(s) 214, 1211, 1607, 1622
AgsI TTSAA 6 cut(s) 28, 317, 928, 1222, 1368, 1537
AhlI ACTAGT 1 cut(s) 1499
AjiI CACGTC 1 cut(s) 673
AjnI CCWGG 3 cut(s) 428, 1331, 1519
Alw26I GTCTC 2 cut(s) 680, 1113
AlwI GGATC 3 cut(s) 104, 1270, 1498
AlwNI CAGNNNCTG 2 cut(s) 1122, 1169
Aor13HI TCCGGA 1 cut(s) 219
AoxI GGCC 4 cut(s) 205, 1349, 1483, 1517
ApaI GGGCCC 1 cut(s) 1487
ApeKI GCWGC 6 cut(s) 652, 749, 1062, 1163, 1166, 1597
ApoI RAATTY 2 cut(s) 137, 661
ArsI GACNNNNNNTTYG 2 cut(s) 1136, 1168
Asp700I GAANNNNTTC 1 cut(s) 1388
AspLEI GCGC 1 cut(s) 407
AspS9I GGNCC 3 cut(s) 217, 1483, 1484
AsuC2I CCSGG 2 cut(s) 1198, 1488
AsuHPI GGTGA 8 cut(s) 133, 271, 301, 643, 680, 691, 715, 875
AsuII TTCGAA 1 cut(s) 1068
AvaII GGWCC 1 cut(s) 217
BaeGI GKGCMC 1 cut(s) 1487
BaeI ACNNNNGTAYC 2 cut(s) 1270, 1303
BalI TGGCCA 1 cut(s) 1519
BanI GGYRCC 1 cut(s) 586
BanII GRGCYC 1 cut(s) 1487
BbvI GCAGC 6 cut(s) 639, 761, 1074, 1175, 1178, 1584
BccI CCATC 6 cut(s) 68, 464, 576, 905, 1298, 1391
BceAI ACGGC 7 cut(s) 192, 220, 425, 665, 1001, 1007, 1248
BcgI CGANNNNNNTGC 2 cut(s) 725, 759
BciT130I CCWGG 3 cut(s) 430, 1333, 1521
BciVI GTATCC 2 cut(s) 127, 1067
BclI TGATCA 1 cut(s) 633
BcnI CCSGG 2 cut(s) 1198, 1488
BcoDI GTCTC 2 cut(s) 680, 1113
BcuI ACTAGT 1 cut(s) 1499
BfaI CTAG 3 cut(s) 563, 1343, 1500
BfmI CTRYAG 2 cut(s) 87, 1114
BfuAI ACCTGC 1 cut(s) 86
BfuI GTATCC 2 cut(s) 127, 1067
BglI GCCNNNNNGGC 1 cut(s) 1232
BisI GCNGC 7 cut(s) 653, 747, 750, 1063, 1164, 1167, 1598
BlpI GCTNAGC 1 cut(s) 1545
BlsI GCNGC 7 cut(s) 654, 748, 751, 1064, 1165, 1168, 1599
Bme1390I CCNGG 5 cut(s) 430, 1198, 1333, 1488, 1521
Bme18I GGWCC 1 cut(s) 217
BmgBI CACGTC 1 cut(s) 673
BmgT120I GGNCC 3 cut(s) 217, 1483, 1484
BmiI GGNNCC 3 cut(s) 588, 1485, 1486
BmrFI CCNGG 5 cut(s) 430, 1198, 1333, 1488, 1521
BmsI GCATC 1 cut(s) 879
BpmI CTGGAG 2 cut(s) 1455, 1629
Bpu10I CCTNAGC 1 cut(s) 1236
Bpu1102I GCTNAGC 1 cut(s) 1545
Bpu14I TTCGAA 1 cut(s) 1068
BpuMI CCSGG 2 cut(s) 1198, 1488
BsaAI YACGTR 1 cut(s) 1211
BsaBI GATNNNNATC 1 cut(s) 794
BsaHI GRCGYC 1 cut(s) 420
BsaJI CCNNGG 5 cut(s) 414, 956, 1196, 1352, 1486
BsaWI WCCGGW 3 cut(s) 219, 754, 1622
Bsc4I CCNNNNNNNGG 4 cut(s) 214, 1211, 1607, 1622
Bse118I RCCGGY 1 cut(s) 1265
Bse1I ACTGG 3 cut(s) 113, 1438, 1612
Bse3DI GCAATG 1 cut(s) 828
Bse8I GATNNNNATC 1 cut(s) 794
BseAI TCCGGA 1 cut(s) 219
BseBI CCWGG 3 cut(s) 430, 1333, 1521
BseDI CCNNGG 5 cut(s) 414, 956, 1196, 1352, 1486
BseGI GGATG 4 cut(s) 60, 475, 587, 1402
BseJI GATNNNNATC 1 cut(s) 794
BseLI CCNNNNNNNGG 4 cut(s) 214, 1211, 1607, 1622
BseMI GCAATG 1 cut(s) 828
BseMII CTCAG 2 cut(s) 448, 1250
BseNI ACTGG 3 cut(s) 113, 1438, 1612
BseRI GAGGAG 7 cut(s) 520, 620, 623, 626, 629, 632, 635
BseSI GKGCMC 1 cut(s) 1487
BseX3I CGGCCG 1 cut(s) 205
BseXI GCAGC 6 cut(s) 639, 761, 1074, 1175, 1178, 1584
BseYI CCCAGC 1 cut(s) 401
Bsh1236I CGCG 2 cut(s) 215, 407
Bsh1285I CGRYCG 1 cut(s) 208
BshFI GGCC 4 cut(s) 207, 1351, 1485, 1519
BshNI GGYRCC 1 cut(s) 586
BsiEI CGRYCG 1 cut(s) 208
BsiSI CCGG 7 cut(s) 220, 755, 996, 1198, 1266, 1488, 1623
BslI CCNNNNNNNGG 4 cut(s) 214, 1211, 1607, 1622
BsmAI GTCTC 2 cut(s) 680, 1113
BsmI GAATGC 1 cut(s) 1049
BsnI GGCC 4 cut(s) 207, 1351, 1485, 1519
Bsp119I TTCGAA 1 cut(s) 1068
Bsp120I GGGCCC 1 cut(s) 1483
Bsp1286I GDGCHC 1 cut(s) 1487
Bsp13I TCCGGA 1 cut(s) 219
Bsp1407I TGTACA 1 cut(s) 1377
Bsp143I GATC 5 cut(s) 77, 109, 633, 1275, 1490
Bsp1720I GCTNAGC 1 cut(s) 1545
Bsp19I CCATGG 2 cut(s) 956, 1352
BspACI CCGC 6 cut(s) 215, 300, 747, 985, 993, 1047
BspANI GGCC 4 cut(s) 207, 1351, 1485, 1519
BspCNI CTCAG 2 cut(s) 447, 1249
BspEI TCCGGA 1 cut(s) 219
BspFNI CGCG 2 cut(s) 215, 407
BspLI GGNNCC 3 cut(s) 588, 1485, 1486
BspMI ACCTGC 1 cut(s) 86
BspPI GGATC 3 cut(s) 104, 1270, 1498
BspT104I TTCGAA 1 cut(s) 1068
BspT107I GGYRCC 1 cut(s) 586
BsrDI GCAATG 1 cut(s) 828
BsrFI RCCGGY 1 cut(s) 1265
BsrGI TGTACA 1 cut(s) 1377
BsrI ACTGG 3 cut(s) 113, 1438, 1612
BssAI RCCGGY 1 cut(s) 1265
BssECI CCNNGG 5 cut(s) 414, 956, 1196, 1352, 1486
BssMI GATC 5 cut(s) 77, 109, 633, 1275, 1490
BssNI GRCGYC 1 cut(s) 420
BssT1I CCWWGG 2 cut(s) 956, 1352
Bst2UI CCWGG 3 cut(s) 430, 1333, 1521
Bst4CI ACNGT 8 cut(s) 691, 697, 883, 1034, 1141, 1286, 1375, 1567
Bst6I CTCTTC 3 cut(s) 597, 810, 1501
BstACI GRCGYC 1 cut(s) 420
BstAUI TGTACA 1 cut(s) 1377
BstBAI YACGTR 1 cut(s) 1211
BstBI TTCGAA 1 cut(s) 1068
BstC8I GCNNGC 3 cut(s) 326, 361, 1551
BstDEI CTNAG 3 cut(s) 434, 1236, 1545
BstDSI CCRYGG 2 cut(s) 956, 1352
BstEII GGTNACC 1 cut(s) 1523
BstF5I GGATG 4 cut(s) 60, 475, 587, 1402
BstFNI CGCG 2 cut(s) 215, 407
BstHHI GCGC 1 cut(s) 407
BstKTI GATC 5 cut(s) 80, 112, 636, 1278, 1493
BstMAI GTCTC 2 cut(s) 680, 1113
BstMBI GATC 5 cut(s) 77, 109, 633, 1275, 1490
BstMCI CGRYCG 1 cut(s) 208
BstMWI GCNNNNNNNGC 5 cut(s) 92, 471, 746, 1232, 1550
BstNI CCWGG 3 cut(s) 430, 1333, 1521
BstNSI RCATGY 1 cut(s) 465
BstPI GGTNACC 1 cut(s) 1523
BstSCI CCNGG 5 cut(s) 428, 1196, 1331, 1486, 1519
BstSFI CTRYAG 2 cut(s) 87, 1114
BstSLI GKGCMC 1 cut(s) 1487
BstUI CGCG 2 cut(s) 215, 407
BstV1I GCAGC 6 cut(s) 639, 761, 1074, 1175, 1178, 1584
BstZI CGGCCG 1 cut(s) 205
BsuI GTATCC 2 cut(s) 127, 1067
BsuRI GGCC 4 cut(s) 207, 1351, 1485, 1519
BtgI CCRYGG 2 cut(s) 956, 1352
BtrI CACGTC 1 cut(s) 673
BtsCI GGATG 4 cut(s) 60, 475, 587, 1402
BtsI GCAGTG 1 cut(s) 975
BtsIMutI CAGTG 4 cut(s) 442, 693, 975, 1572
BveI ACCTGC 1 cut(s) 86
Cac8I GCNNGC 3 cut(s) 326, 361, 1551
CaiI CAGNNNCTG 2 cut(s) 1122, 1169
CfoI GCGC 1 cut(s) 407
Cfr10I RCCGGY 1 cut(s) 1265
Cfr13I GGNCC 3 cut(s) 217, 1483, 1484
CpoI CGGWCCG 1 cut(s) 217
CseI GACGC 1 cut(s) 746
Csp6I GTAC 3 cut(s) 476, 1287, 1378
CspCI CAANNNNNGTGG 2 cut(s) 924, 959
CspI CGGWCCG 1 cut(s) 217
CviAII CATG 8 cut(s) 169, 462, 639, 957, 1353, 1381, 1393, 1515
CviQI GTAC 3 cut(s) 476, 1287, 1378
DdeI CTNAG 3 cut(s) 434, 1236, 1545
DpnI GATC 5 cut(s) 79, 111, 635, 1277, 1492
DpnII GATC 5 cut(s) 77, 109, 633, 1275, 1490
DraI TTTAAA 1 cut(s) 774
DrdI GACNNNNNNGTC 1 cut(s) 1127
DseDI GACNNNNNNGTC 1 cut(s) 1127
EaeI YGGCCR 2 cut(s) 205, 1517
EagI CGGCCG 1 cut(s) 205
Eam1104I CTCTTC 3 cut(s) 597, 810, 1501
EarI CTCTTC 3 cut(s) 597, 810, 1501
EclXI CGGCCG 1 cut(s) 205
Eco130I CCWWGG 2 cut(s) 956, 1352
Eco24I GRGCYC 1 cut(s) 1487
Eco47I GGWCC 1 cut(s) 217
Eco52I CGGCCG 1 cut(s) 205
Eco57I CTGAAG 1 cut(s) 641
Eco91I GGTNACC 1 cut(s) 1523
EcoO109I RGGNCCY 2 cut(s) 1483, 1484
EcoO65I GGTNACC 1 cut(s) 1523
EcoRI GAATTC 1 cut(s) 137
EcoRII CCWGG 3 cut(s) 428, 1331, 1519
EcoT14I CCWWGG 2 cut(s) 956, 1352
EcoT38I GRGCYC 1 cut(s) 1487
ErhI CCWWGG 2 cut(s) 956, 1352
FaeI CATG 8 cut(s) 172, 465, 642, 960, 1356, 1384, 1396, 1518
FatI CATG 8 cut(s) 168, 461, 638, 956, 1352, 1380, 1392, 1514
FbaI TGATCA 1 cut(s) 633
FblI GTMKAC 2 cut(s) 1131, 1143
Fnu4HI GCNGC 7 cut(s) 653, 747, 750, 1063, 1164, 1167, 1598
FokI GGATG 4 cut(s) 47, 482, 594, 1409
FriOI GRGCYC 1 cut(s) 1487
Fsp4HI GCNGC 7 cut(s) 653, 747, 750, 1063, 1164, 1167, 1598
FspBI CTAG 3 cut(s) 563, 1343, 1500
GlaI GCGC 1 cut(s) 406
GluI GCNGC 7 cut(s) 653, 747, 750, 1063, 1164, 1167, 1598
GsaI CCCAGC 1 cut(s) 405
GsuI CTGGAG 2 cut(s) 1455, 1629
HaeIII GGCC 4 cut(s) 207, 1351, 1485, 1519
HapII CCGG 7 cut(s) 220, 755, 996, 1198, 1266, 1488, 1623
HgaI GACGC 1 cut(s) 746
HhaI GCGC 1 cut(s) 407
Hin1I GRCGYC 1 cut(s) 420
Hin1II CATG 8 cut(s) 172, 465, 642, 960, 1356, 1384, 1396, 1518
Hin6I GCGC 1 cut(s) 405
HinP1I GCGC 1 cut(s) 405
HincII GTYRAC 1 cut(s) 760
HindII GTYRAC 1 cut(s) 760
HindIII AAGCTT 1 cut(s) 326
HinfI GANTC 3 cut(s) 37, 700, 1255
HpaI GTTAAC 1 cut(s) 760
HpaII CCGG 7 cut(s) 220, 755, 996, 1198, 1266, 1488, 1623
HphI GGTGA 8 cut(s) 133, 271, 301, 643, 680, 691, 715, 875
Hpy188I TCNGA 8 cut(s) 660, 852, 900, 1028, 1206, 1295, 1303, 1318
Hpy188III TCNNGA 6 cut(s) 34, 75, 220, 574, 704, 787
Hpy99I CGWCG 2 cut(s) 740, 1022
HpyAV CCTTC 7 cut(s) 162, 571, 991, 1064, 1351, 1399, 1409
HpyCH4III ACNGT 8 cut(s) 691, 697, 883, 1034, 1141, 1286, 1375, 1567
HpyCH4IV ACGT 5 cut(s) 345, 420, 672, 1128, 1210
HpyF10VI GCNNNNNNNGC 5 cut(s) 92, 471, 746, 1232, 1550
HpyF3I CTNAG 3 cut(s) 434, 1236, 1545
HpySE526I ACGT 5 cut(s) 345, 420, 672, 1128, 1210
Hsp92I GRCGYC 1 cut(s) 420
Hsp92II CATG 8 cut(s) 172, 465, 642, 960, 1356, 1384, 1396, 1518
HspAI GCGC 1 cut(s) 405
Kpn2I TCCGGA 1 cut(s) 219
Ksp22I TGATCA 1 cut(s) 633
KspAI GTTAAC 1 cut(s) 760
Kzo9I GATC 5 cut(s) 77, 109, 633, 1275, 1490
LmnI GCTCC 3 cut(s) 368, 757, 998
Lsp1109I GCAGC 6 cut(s) 639, 761, 1074, 1175, 1178, 1584
LweI GCATC 1 cut(s) 879
MaeI CTAG 3 cut(s) 563, 1343, 1500
MaeII ACGT 5 cut(s) 345, 420, 672, 1128, 1210
MaeIII GTNAC 7 cut(s) 209, 668, 691, 779, 1184, 1510, 1523
MalI GATC 5 cut(s) 79, 111, 635, 1277, 1492
MboI GATC 5 cut(s) 77, 109, 633, 1275, 1490
MboII GAAGA 8 cut(s) 38, 329, 480, 483, 614, 827, 1518, 1600
MhlI GDGCHC 1 cut(s) 1487
MlsI TGGCCA 1 cut(s) 1519
MluCI AATT 7 cut(s) 137, 245, 661, 928, 948, 1057, 1109
MluNI TGGCCA 1 cut(s) 1519
MlyI GAGTC 2 cut(s) 31, 709
MmeI TCCRAC 3 cut(s) 298, 558, 1400
Mox20I TGGCCA 1 cut(s) 1519
MroI TCCGGA 1 cut(s) 219
MroXI GAANNNNTTC 1 cut(s) 1388
MscI TGGCCA 1 cut(s) 1519
MseI TTAA 4 cut(s) 252, 759, 773, 1448
Msp20I TGGCCA 1 cut(s) 1519
MspA1I CMGCKG 1 cut(s) 1169
MspI CCGG 7 cut(s) 220, 755, 996, 1198, 1266, 1488, 1623
MspR9I CCNGG 5 cut(s) 430, 1198, 1333, 1488, 1521
Mva1269I GAATGC 1 cut(s) 1049
MvaI CCWGG 3 cut(s) 430, 1333, 1521
MvnI CGCG 2 cut(s) 215, 407
MwoI GCNNNNNNNGC 5 cut(s) 92, 471, 746, 1232, 1550
NciI CCSGG 2 cut(s) 1198, 1488
NcoI CCATGG 2 cut(s) 956, 1352
NdeII GATC 5 cut(s) 77, 109, 633, 1275, 1490
NlaIII CATG 8 cut(s) 172, 465, 642, 960, 1356, 1384, 1396, 1518
NlaIV GGNNCC 3 cut(s) 588, 1485, 1486
NmeAIII GCCGAG 2 cut(s) 439, 1492
NmuCI GTSAC 2 cut(s) 668, 691
NspI RCATGY 1 cut(s) 465
NspV TTCGAA 1 cut(s) 1068
PctI GAATGC 1 cut(s) 1049
PdmI GAANNNNTTC 1 cut(s) 1388
PfeI GAWTC 1 cut(s) 1255
PkrI GCNGC 7 cut(s) 654, 748, 751, 1064, 1165, 1168, 1599
PleI GAGTC 2 cut(s) 31, 708
PpsI GAGTC 2 cut(s) 31, 708
Ppu21I YACGTR 1 cut(s) 1211
Psp6I CCWGG 3 cut(s) 428, 1331, 1519
PspEI GGTNACC 1 cut(s) 1523
PspFI CCCAGC 1 cut(s) 401
PspGI CCWGG 3 cut(s) 428, 1331, 1519
PspN4I GGNNCC 3 cut(s) 588, 1485, 1486
PspOMI GGGCCC 1 cut(s) 1483
PspPI GGNCC 3 cut(s) 217, 1483, 1484
PstNI CAGNNNCTG 2 cut(s) 1122, 1169
PvuII CAGCTG 1 cut(s) 1169
RsaI GTAC 3 cut(s) 477, 1288, 1379
RsaNI GTAC 3 cut(s) 476, 1287, 1378
Rsr2I CGGWCCG 1 cut(s) 217
RsrII CGGWCCG 1 cut(s) 217
SaqAI TTAA 4 cut(s) 252, 759, 773, 1448
SatI GCNGC 7 cut(s) 653, 747, 750, 1063, 1164, 1167, 1598
Sau3AI GATC 5 cut(s) 77, 109, 633, 1275, 1490
Sau96I GGNCC 3 cut(s) 217, 1483, 1484
SchI GAGTC 2 cut(s) 31, 709
ScrFI CCNGG 5 cut(s) 430, 1198, 1333, 1488, 1521
SduI GDGCHC 1 cut(s) 1487
SfaNI GCATC 1 cut(s) 879
SfcI CTRYAG 2 cut(s) 87, 1114
SfuI TTCGAA 1 cut(s) 1068
SinI GGWCC 1 cut(s) 217
SpeI ACTAGT 1 cut(s) 1499
Sse9I AATT 7 cut(s) 137, 245, 661, 928, 948, 1057, 1109
SsiI CCGC 6 cut(s) 215, 300, 747, 985, 993, 1047
SspMI CTAG 3 cut(s) 563, 1343, 1500
StyD4I CCNGG 5 cut(s) 428, 1196, 1331, 1486, 1519
StyI CCWWGG 2 cut(s) 956, 1352
TaaI ACNGT 8 cut(s) 691, 697, 883, 1034, 1141, 1286, 1375, 1567
TaiI ACGT 5 cut(s) 348, 423, 675, 1131, 1213
TaqI TCGA 5 cut(s) 35, 76, 1068, 1243, 1493
TasI AATT 7 cut(s) 137, 245, 661, 928, 948, 1057, 1109
TatI WGTACW 1 cut(s) 1377
TauI GCSGC 1 cut(s) 749
TfiI GAWTC 1 cut(s) 1255
Tru1I TTAA 4 cut(s) 252, 759, 773, 1448
Tru9I TTAA 4 cut(s) 252, 759, 773, 1448
TscAI CASTG 4 cut(s) 442, 700, 975, 1572
TseFI GTSAC 2 cut(s) 668, 691
TseI GCWGC 6 cut(s) 652, 749, 1062, 1163, 1166, 1597
Tsp45I GTSAC 2 cut(s) 668, 691
TspDTI ATGAA 8 cut(s) 38, 144, 483, 527, 828, 1138, 1381, 1397
TspGWI ACGGA 2 cut(s) 723, 1149
TspRI CASTG 4 cut(s) 442, 700, 975, 1572
VpaK11BI GGWCC 1 cut(s) 217
XapI RAATTY 2 cut(s) 137, 661
XceI RCATGY 1 cut(s) 465
XmiI GTMKAC 2 cut(s) 1131, 1143
XmnI GAANNNNTTC 1 cut(s) 1388
XspI CTAG 3 cut(s) 563, 1343, 1500
ZraI GACGTC 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.