RLG00000001035

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000522
Physical Location & Seq
Forward (+)
9512959 .. 9514782
1824 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001035

Sequence Viewer

Length: 1698 bp
ATGGCTTCTAAGCTCTTCAATCCTCTTGTAACCTCATCATTTTCACTATTCCTCATCATTTTCGCTTTGTTTATATCTCAAACTTCAGCTGCAGATGTTCCTCTCAGCATTTCTGTTCCGCCCGAAACCATATGCAAGTCCACTCCACACCCTTCTGTTTGCATTTCCGCCCTCCCTCACAAAAATGCCACTGTCTATGACTTCGGCCGCTTGTCAGTCCAACACTCATTCGTCCAATCCCAAAAGCTTCTGGATTTGATCAACAAATATGTTCAAGGTGGTTCCAGTTTATCACAGCTGGAAATCCAAGCCCTCGAGGATTGCAAGTCGCTTGCATTGTTAAACATTGATTTCTTATCAAACACTCTTGAAACTGTTAACAAAACAAGCGGTGTTCTTCCTAGCTCCGATGCTGACGATGTCCAAACCCTGCTTAGTGCCATCTTAACCAACCAGCAGACGTGTTCTGATGGCCTTCAATCTGTGAGTTCAGTCCAAAATGATCTCTCAGTATCCGTTTCTAATGGTACAAATTTGTACAGTGTCTCATTGGCGTTGGTTACAAAGGGTTGGGTCCCAAAGGATCTCAAAAATAATGGAACACCAAACCAGTCGAAAAAGCATCTCAGTTTCCGAAAGGGGCGATTGTCACTGAAAATGTCAAGCGAAGCACGTGCAGTTTACGACTCGGTTATAAATCGAAGAAGGCACCTTCTTCAAGCCGGAGATGAAGAAATTTTGGTGAAGGACATTGTGGTTGTGAGCCAAGATGGAAGTGGAAACTTCACAACCATCAATGATGCTATCAATGCTGCACCCAATAACTCTGTTGCTAGTGATGGCTACTTCTTGGTATATGTCACTGCCGGCGTTTATCAAGAGTACGTATCAATTATATCGAAAAAGAAGTACTTGTTGCTGATCGGAGATGGTATTAATCAGACGATCATCACAGGAAACAACAGCGTTGGAGGCGGTTCAACCACCTTCAACTCTGCCACTTTAGCTGTGGTAGCAACAGGGTTTATAGCAGTCAACATAACTGTTCGAAACACTGCTGGACCAAGCATGGGGCAAGCAGTTGCAGTTCGAAATGGAGCTGACTTGTCAGTTTTCTACATCTGTAGCTTTGAGGGATACCAAGACACCTTATACACACATTCTATGAGACAATTCTACAGGGAATGTAACATCTACGGAACGGTTGATTTCATCTTTGGAAATGCTGCAGTTGTTTTCCAAAACTGCAACATATACCCTCGTCGTCCCCTCCAAGGCCAATCAGATACCATCACAGCTCAAGGGCGGACCGATGTGAATCAGAACACAGGGACTTCAATCCAGAATTGCACCATTACAGCCGCGGCTGATTTGGCTTCAAGTAACTTCACTGTCAAGACATATCTTGGGAGGCCATGGAAGGAGTATTCGAGGACGGTTTACTTGCAGAATTTCATGGATAGTTTGATCGATCCTGCTGGTTGGCTCGCGTGGAGTGGAGATTTTGCACTGAGCACATTGTATTATGCTGAGTACAATAATAGAGGACCTGGATCAAACACTAGCAACAGAGTTACATGGCCTGGTTACCATGTGATCGGTGCAACTGATGCTGCCAACTTTACAGTCTCCAACTTCATATTGGGGGATAATTGGCTGCCTCAGACAGGAGTGCCTTACACTGGTGGCTTAATTTGA

Protein Analysis

566

Amino Acids

61.15

Weight (kDa)

6.18

Isoelectric Point (pI)

33.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 42 - 186 1.5e-25 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 252 - 550 1.7e-135 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000522)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02810 AT2G47550 AT4G02330
fragaria_vesca FvH4_2g25960 FvH4_2g25960 FvH4_2g25970 FvH4_5g35570 FvH4_5g35580 FvH4_5g35590
malus_domestica MD03G1290900.v1.1 MD03G1291000.v1.1 MD08G1195600.v1.1 MD11G1307400.v1.1 MD11G1307500.v1.1
prunus_persica Prupe.1G529400_v2.0.a1 Prupe.1G529500_v2.0.a1 Prupe.8G263900_v2.0.a1 Prupe.8G264000_v2.0.a1
pyrus_communis pycom08g16800
rosa_chinensis RchiOBHm_Chr6g0294321 RchiOBHm_Chr6g0294331 RchiOBHm_Chr7g0236951 RchiOBHm_Chr7g0236971 RchiOBHm_Chr7g0237041 RchiOBHm_Chr7g0237051
rosa_laevigata RLG00000001034 RLG00000001035 RLG00000001039 RLG00000001043 RLG00000001045 RLG00000011880 RLG00000011881 RLG00000034181
rosa_multiflora Rmu_co8086188.1_g000001 Rmu_co8458485.1_g000001 Rmu_sc0000272.1_g000007 Rmu_sc0000272.1_g000009 Rmu_sc0002131.1_g000030 Rmu_sc0002131.1_g000037 Rmu_sc0005369.1_g000005 Rmu_sc0005369.1_g000008 Rmu_sc0007991.1_g000005 Rmu_sc0007991.1_g000013 Rmu_sc0033563.1_g000001
rosa_roxburghii Rroxscaffold_3G00224540 Rroxscaffold_3G00224600 Rroxscaffold_3G00224650 Rroxscaffold_3G00224660 Rroxscaffold_7G00173470 Rroxscaffold_7G00173480
rosa_rugosa Rorug06G0243400 Rorug06G0243500 Rorug07G0301200 Rorug07G0301500 Rorug07G0301600
rosa_samantha Rh6AG356000 Rh6AG356100 Rh6BG362600 Rh6BG362800 Rh6CG370000 Rh6CG370100 Rh6DG356500 Rh6DG356600 Rh7AG456200 Rh7AG456500 Rh7AG457500 Rh7AG457600 Rh7BG427500 Rh7BG427700 Rh7BG428400 Rh7BG428500 Rh7CG475100 Rh7CG475300 Rh7CG475700 Rh7CG475800 Rh7DG444100 Rh7DG444300 Rh7DG444700 Rh7DG444900
rosa_wichuraiana Rw6G031020 Rw6G031030 Rw7G037870 Rw7G037880 Rw7G037920 Rw7G037940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 695
AbsI CCTCGAGG 1 cut(s) 314
AccB1I GGYRCC 1 cut(s) 708
AccII CGCG 2 cut(s) 1364, 1490
AciI CCGC 8 cut(s) 119, 168, 208, 390, 975, 1306, 1362, 1364
AclWI GGATC 3 cut(s) 591, 1466, 1561
AcoI YGGCCR 1 cut(s) 205
AcsI RAATTY 3 cut(s) 532, 735, 1450
AcuI CTGAAG 1 cut(s) 69
AcvI CACGTG 1 cut(s) 674
AfaI GTAC 5 cut(s) 529, 539, 884, 911, 1535
AfiI CCNNNNNNNGG 4 cut(s) 1070, 1274, 1667, 1682
AflIII ACRYGT 1 cut(s) 461
AgsI TTSAA 9 cut(s) 19, 275, 371, 479, 719, 981, 991, 1338, 1380
AjiI CACGTC 1 cut(s) 462
AjnI CCWGG 2 cut(s) 1549, 1582
AluBI AGCT 9 cut(s) 13, 89, 247, 298, 405, 1007, 1100, 1128, 1298
AluI AGCT 9 cut(s) 13, 89, 247, 298, 405, 1007, 1100, 1128, 1298
Alw21I GWGCWC 1 cut(s) 1517
Alw26I GTCTC 3 cut(s) 550, 1162, 1633
AlwI GGATC 3 cut(s) 591, 1466, 1561
Ama87I CYCGRG 1 cut(s) 314
AoxI GGCC 5 cut(s) 205, 472, 1276, 1412, 1580
ApeKI GCWGC 5 cut(s) 89, 812, 1226, 1613, 1657
ApoI RAATTY 3 cut(s) 532, 735, 1450
AseI ATTAAT 1 cut(s) 936
AspS9I GGNCC 4 cut(s) 574, 1061, 1308, 1547
AsuHPI GGTGA 1 cut(s) 754
AsuII TTCGAA 2 cut(s) 1048, 1090
AvaI CYCGRG 1 cut(s) 314
AvaII GGWCC 4 cut(s) 574, 1061, 1308, 1547
BanI GGYRCC 1 cut(s) 708
BbrPI CACGTG 1 cut(s) 674
Bbv12I GWGCWC 1 cut(s) 1517
BbvI GCAGC 5 cut(s) 76, 799, 1213, 1600, 1644
BccI CCATC 7 cut(s) 449, 464, 764, 800, 833, 923, 1298
BcgI CGANNNNNNTGC 2 cut(s) 656, 690
BciT130I CCWGG 2 cut(s) 1551, 1584
BciVI GTATCC 2 cut(s) 523, 1130
BclI TGATCA 1 cut(s) 258
BcoDI GTCTC 3 cut(s) 550, 1162, 1633
BfaI CTAG 3 cut(s) 402, 834, 1563
BfmI CTRYAG 4 cut(s) 90, 1123, 1177, 1227
BfuI GTATCC 2 cut(s) 523, 1130
BisI GCNGC 8 cut(s) 90, 208, 813, 1227, 1362, 1365, 1614, 1658
BlsI GCNGC 8 cut(s) 91, 209, 814, 1228, 1363, 1366, 1615, 1659
BmcAI AGTACT 1 cut(s) 911
Bme1390I CCNGG 2 cut(s) 1551, 1584
Bme18I GGWCC 4 cut(s) 574, 1061, 1308, 1547
BmeT110I CYCGRG 1 cut(s) 314
BmgBI CACGTC 1 cut(s) 462
BmgT120I GGNCC 4 cut(s) 574, 1061, 1308, 1547
BmiI GGNNCC 4 cut(s) 283, 575, 576, 710
BmrFI CCNGG 2 cut(s) 1551, 1584
BmsI GCATC 4 cut(s) 400, 631, 790, 1600
Bpu14I TTCGAA 2 cut(s) 1048, 1090
BpuEI CTTGAG 1 cut(s) 1284
Bsa29I ATCGAT 1 cut(s) 1470
BsaAI YACGTR 2 cut(s) 674, 886
BsaBI GATNNNNATC 1 cut(s) 1317
BsaJI CCNNGG 3 cut(s) 1273, 1362, 1415
Bsc4I CCNNNNNNNGG 4 cut(s) 1070, 1274, 1667, 1682
Bse118I RCCGGY 1 cut(s) 866
Bse1I ACTGG 3 cut(s) 285, 610, 1687
Bse8I GATNNNNATC 1 cut(s) 1317
BseBI CCWGG 2 cut(s) 1551, 1584
BseCI ATCGAT 1 cut(s) 1470
BseDI CCNNGG 3 cut(s) 1273, 1362, 1415
BseJI GATNNNNATC 1 cut(s) 1317
BseLI CCNNNNNNNGG 4 cut(s) 1070, 1274, 1667, 1682
BseMII CTCAG 6 cut(s) 118, 522, 640, 1502, 1521, 1676
BseNI ACTGG 3 cut(s) 285, 610, 1687
BseX3I CGGCCG 1 cut(s) 205
BseXI GCAGC 5 cut(s) 76, 799, 1213, 1600, 1644
BsgI GTGCAG 2 cut(s) 696, 798
Bsh1236I CGCG 2 cut(s) 1364, 1490
Bsh1285I CGRYCG 1 cut(s) 208
BshFI GGCC 5 cut(s) 207, 474, 1278, 1414, 1582
BshNI GGYRCC 1 cut(s) 708
BshVI ATCGAT 1 cut(s) 1470
BsiEI CGRYCG 1 cut(s) 208
BsiHKAI GWGCWC 1 cut(s) 1517
BsiHKCI CYCGRG 1 cut(s) 314
BsiSI CCGG 2 cut(s) 723, 867
BslFI GGGAC 3 cut(s) 560, 1251, 1345
BslI CCNNNNNNNGG 4 cut(s) 1070, 1274, 1667, 1682
BsmAI GTCTC 3 cut(s) 550, 1162, 1633
BsmFI GGGAC 3 cut(s) 560, 1251, 1345
BsnI GGCC 5 cut(s) 207, 474, 1278, 1414, 1582
BsoBI CYCGRG 1 cut(s) 314
Bsp119I TTCGAA 2 cut(s) 1048, 1090
Bsp1286I GDGCHC 1 cut(s) 1517
Bsp1407I TGTACA 1 cut(s) 537
Bsp143I GATC 9 cut(s) 258, 502, 583, 921, 945, 1467, 1471, 1553, 1596
Bsp19I CCATGG 1 cut(s) 1415
BspACI CCGC 8 cut(s) 119, 168, 208, 390, 975, 1306, 1362, 1364
BspANI GGCC 5 cut(s) 207, 474, 1278, 1414, 1582
BspCNI CTCAG 6 cut(s) 117, 521, 639, 1503, 1522, 1675
BspDI ATCGAT 1 cut(s) 1470
BspFNI CGCG 2 cut(s) 1364, 1490
BspLI GGNNCC 4 cut(s) 283, 575, 576, 710
BspMAI CTGCAG 2 cut(s) 94, 1231
BspPI GGATC 3 cut(s) 591, 1466, 1561
BspQI GCTCTTC 1 cut(s) 20
BspT104I TTCGAA 2 cut(s) 1048, 1090
BspT107I GGYRCC 1 cut(s) 708
BsrFI RCCGGY 1 cut(s) 866
BsrGI TGTACA 1 cut(s) 537
BsrI ACTGG 3 cut(s) 285, 610, 1687
BssAI RCCGGY 1 cut(s) 866
BssECI CCNNGG 3 cut(s) 1273, 1362, 1415
BssMI GATC 9 cut(s) 258, 502, 583, 921, 945, 1467, 1471, 1553, 1596
BssT1I CCWWGG 2 cut(s) 1273, 1415
Bst2UI CCWGG 2 cut(s) 1551, 1584
Bst4CI ACNGT 8 cut(s) 193, 376, 542, 1045, 1204, 1393, 1438, 1627
Bst6I CTCTTC 1 cut(s) 20
BstAPI GCANNNNNTGC 1 cut(s) 1610
BstAUI TGTACA 1 cut(s) 537
BstBAI YACGTR 2 cut(s) 674, 886
BstBI TTCGAA 2 cut(s) 1048, 1090
BstC8I GCNNGC 4 cut(s) 333, 868, 1077, 1488
BstDEI CTNAG 8 cut(s) 9, 104, 434, 508, 626, 1511, 1530, 1662
BstDSI CCRYGG 2 cut(s) 1362, 1415
BstEII GGTNACC 1 cut(s) 1586
BstENI CCTNNNNNAGG 1 cut(s) 1665
BstFNI CGCG 2 cut(s) 1364, 1490
BstKTI GATC 9 cut(s) 261, 505, 586, 924, 948, 1470, 1474, 1556, 1599
BstMAI GTCTC 3 cut(s) 550, 1162, 1633
BstMBI GATC 9 cut(s) 258, 502, 583, 921, 945, 1467, 1471, 1553, 1596
BstMCI CGRYCG 1 cut(s) 208
BstMWI GCNNNNNNNGC 6 cut(s) 809, 972, 1004, 1013, 1373, 1610
BstNI CCWGG 2 cut(s) 1551, 1584
BstPI GGTNACC 1 cut(s) 1586
BstSCI CCNGG 2 cut(s) 1549, 1582
BstSFI CTRYAG 4 cut(s) 90, 1123, 1177, 1227
BstSNI TACGTA 1 cut(s) 886
BstUI CGCG 2 cut(s) 1364, 1490
BstV1I GCAGC 5 cut(s) 76, 799, 1213, 1600, 1644
BstX2I RGATCY 1 cut(s) 583
BstYI RGATCY 1 cut(s) 583
BstZI CGGCCG 1 cut(s) 205
Bsu15I ATCGAT 1 cut(s) 1470
BsuI GTATCC 2 cut(s) 523, 1130
BsuRI GGCC 5 cut(s) 207, 474, 1278, 1414, 1582
BsuTUI ATCGAT 1 cut(s) 1470
BtgI CCRYGG 2 cut(s) 1362, 1415
BtrI CACGTC 1 cut(s) 462
BtsI GCAGTG 2 cut(s) 861, 1053
BtsIMutI CAGTG 8 cut(s) 189, 547, 650, 861, 1053, 1389, 1508, 1680
Cac8I GCNNGC 4 cut(s) 333, 868, 1077, 1488
Cfr10I RCCGGY 1 cut(s) 866
Cfr13I GGNCC 4 cut(s) 574, 1061, 1308, 1547
Cfr42I CCGCGG 1 cut(s) 1365
ClaI ATCGAT 1 cut(s) 1470
CpoI CGGWCCG 1 cut(s) 1308
Csp6I GTAC 5 cut(s) 528, 538, 883, 910, 1534
CspI CGGWCCG 1 cut(s) 1308
CviAII CATG 5 cut(s) 1069, 1416, 1456, 1578, 1592
CviQI GTAC 5 cut(s) 528, 538, 883, 910, 1534
DdeI CTNAG 8 cut(s) 9, 104, 434, 508, 626, 1511, 1530, 1662
DpnI GATC 9 cut(s) 260, 504, 585, 923, 947, 1469, 1473, 1555, 1598
DpnII GATC 9 cut(s) 258, 502, 583, 921, 945, 1467, 1471, 1553, 1596
EaeI YGGCCR 1 cut(s) 205
EagI CGGCCG 1 cut(s) 205
Eam1104I CTCTTC 1 cut(s) 20
EarI CTCTTC 1 cut(s) 20
EciI GGCGGA 3 cut(s) 108, 157, 1321
EclXI CGGCCG 1 cut(s) 205
Eco105I TACGTA 1 cut(s) 886
Eco130I CCWWGG 2 cut(s) 1273, 1415
Eco47I GGWCC 4 cut(s) 574, 1061, 1308, 1547
Eco52I CGGCCG 1 cut(s) 205
Eco57I CTGAAG 1 cut(s) 69
Eco72I CACGTG 1 cut(s) 674
Eco88I CYCGRG 1 cut(s) 314
Eco91I GGTNACC 1 cut(s) 1586
EcoNI CCTNNNNNAGG 1 cut(s) 1665
EcoO109I RGGNCCY 2 cut(s) 574, 1547
EcoO65I GGTNACC 1 cut(s) 1586
EcoRII CCWGG 2 cut(s) 1549, 1582
EcoT14I CCWWGG 2 cut(s) 1273, 1415
ErhI CCWWGG 2 cut(s) 1273, 1415
FaeI CATG 5 cut(s) 1072, 1419, 1459, 1581, 1595
FalI AAGNNNNNCTT 2 cut(s) 896, 928
FaqI GGGAC 3 cut(s) 560, 1251, 1345
FatI CATG 5 cut(s) 1068, 1415, 1455, 1577, 1591
FauNDI CATATG 1 cut(s) 131
FbaI TGATCA 1 cut(s) 258
Fnu4HI GCNGC 8 cut(s) 90, 208, 813, 1227, 1362, 1365, 1614, 1658
Fsp4HI GCNGC 8 cut(s) 90, 208, 813, 1227, 1362, 1365, 1614, 1658
FspBI CTAG 3 cut(s) 402, 834, 1563
GluI GCNGC 8 cut(s) 90, 208, 813, 1227, 1362, 1365, 1614, 1658
HaeIII GGCC 5 cut(s) 207, 474, 1278, 1414, 1582
HapII CCGG 2 cut(s) 723, 867
Hin1II CATG 5 cut(s) 1072, 1419, 1459, 1581, 1595
HincII GTYRAC 2 cut(s) 379, 1036
HindII GTYRAC 2 cut(s) 379, 1036
HindIII AAGCTT 1 cut(s) 245
HinfI GANTC 2 cut(s) 686, 1318
HpaI GTTAAC 1 cut(s) 379
HpaII CCGG 2 cut(s) 723, 867
HphI GGTGA 1 cut(s) 754
Hpy166II GTNNAC 5 cut(s) 141, 379, 682, 1036, 1441
Hpy188I TCNGA 8 cut(s) 409, 469, 635, 926, 942, 1285, 1323, 1665
Hpy188III TCNNGA 5 cut(s) 251, 368, 878, 1342, 1396
Hpy8I GTNNAC 5 cut(s) 141, 379, 682, 1036, 1441
Hpy99I CGWCG 1 cut(s) 1266
HpyAV CCTTC 7 cut(s) 162, 485, 699, 722, 739, 997, 1414
HpyCH4III ACNGT 8 cut(s) 193, 376, 542, 1045, 1204, 1393, 1438, 1627
HpyCH4IV ACGT 3 cut(s) 461, 673, 885
HpyF10VI GCNNNNNNNGC 6 cut(s) 809, 972, 1004, 1013, 1373, 1610
HpyF3I CTNAG 8 cut(s) 9, 104, 434, 508, 626, 1511, 1530, 1662
HpySE526I ACGT 3 cut(s) 461, 673, 885
Hsp92II CATG 5 cut(s) 1072, 1419, 1459, 1581, 1595
KflI GGGWCCC 1 cut(s) 574
KroI GCCGGC 1 cut(s) 866
KroNI GCCGGC 1 cut(s) 868
Ksp22I TGATCA 1 cut(s) 258
KspAI GTTAAC 1 cut(s) 379
KspI CCGCGG 1 cut(s) 1365
Kzo9I GATC 9 cut(s) 258, 502, 583, 921, 945, 1467, 1471, 1553, 1596
LguI GCTCTTC 1 cut(s) 20
LmnI GCTCC 2 cut(s) 410, 1097
Lsp1109I GCAGC 5 cut(s) 76, 799, 1213, 1600, 1644
LweI GCATC 4 cut(s) 400, 631, 790, 1600
MaeI CTAG 3 cut(s) 402, 834, 1563
MaeII ACGT 3 cut(s) 461, 673, 885
MaeIII GTNAC 8 cut(s) 28, 559, 648, 859, 1187, 1382, 1573, 1586
MalI GATC 9 cut(s) 260, 504, 585, 923, 947, 1469, 1473, 1555, 1598
MboI GATC 9 cut(s) 258, 502, 583, 921, 945, 1467, 1471, 1553, 1596
MboII GAAGA 5 cut(s) 7, 389, 707, 714, 743
MflI RGATCY 1 cut(s) 583
MhlI GDGCHC 1 cut(s) 1517
MluCI AATT 8 cut(s) 532, 735, 891, 1172, 1345, 1450, 1651, 1692
MlyI GAGTC 1 cut(s) 680
MmeI TCCRAC 3 cut(s) 244, 949, 1656
MroNI GCCGGC 1 cut(s) 866
MseI TTAA 5 cut(s) 341, 378, 446, 936, 1691
MslI CAYNNNNRTG 1 cut(s) 183
MspA1I CMGCKG 3 cut(s) 89, 298, 1364
MspI CCGG 2 cut(s) 723, 867
MspR9I CCNGG 2 cut(s) 1551, 1584
MvaI CCWGG 2 cut(s) 1551, 1584
MvnI CGCG 2 cut(s) 1364, 1490
MwoI GCNNNNNNNGC 6 cut(s) 809, 972, 1004, 1013, 1373, 1610
NaeI GCCGGC 1 cut(s) 868
NcoI CCATGG 1 cut(s) 1415
NdeI CATATG 1 cut(s) 131
NdeII GATC 9 cut(s) 258, 502, 583, 921, 945, 1467, 1471, 1553, 1596
NgoMIV GCCGGC 1 cut(s) 866
NlaIII CATG 5 cut(s) 1072, 1419, 1459, 1581, 1595
NlaIV GGNNCC 4 cut(s) 283, 575, 576, 710
NmuCI GTSAC 2 cut(s) 648, 859
NspV TTCGAA 2 cut(s) 1048, 1090
PaeR7I CTCGAG 1 cut(s) 314
PciSI GCTCTTC 1 cut(s) 20
PdiI GCCGGC 1 cut(s) 868
PfeI GAWTC 1 cut(s) 1318
PflFI GACNNNGTC 1 cut(s) 419
PkrI GCNGC 8 cut(s) 91, 209, 814, 1228, 1363, 1366, 1615, 1659
PleI GAGTC 1 cut(s) 680
PmaCI CACGTG 1 cut(s) 674
PmlI CACGTG 1 cut(s) 674
PpsI GAGTC 1 cut(s) 680
Ppu21I YACGTR 2 cut(s) 674, 886
PpuMI RGGWCCY 2 cut(s) 574, 1547
PshBI ATTAAT 1 cut(s) 936
PsiI TTATAA 1 cut(s) 695
Psp5II RGGWCCY 2 cut(s) 574, 1547
Psp6I CCWGG 2 cut(s) 1549, 1582
PspCI CACGTG 1 cut(s) 674
PspEI GGTNACC 1 cut(s) 1586
PspGI CCWGG 2 cut(s) 1549, 1582
PspN4I GGNNCC 4 cut(s) 283, 575, 576, 710
PspPI GGNCC 4 cut(s) 574, 1061, 1308, 1547
PspPPI RGGWCCY 2 cut(s) 574, 1547
PspXI VCTCGAGB 1 cut(s) 314
PstI CTGCAG 2 cut(s) 94, 1231
PsuI RGATCY 1 cut(s) 583
PsyI GACNNNGTC 1 cut(s) 419
PvuII CAGCTG 2 cut(s) 89, 298
RsaI GTAC 5 cut(s) 529, 539, 884, 911, 1535
RsaNI GTAC 5 cut(s) 528, 538, 883, 910, 1534
RseI CAYNNNNRTG 1 cut(s) 183
Rsr2I CGGWCCG 1 cut(s) 1308
RsrII CGGWCCG 1 cut(s) 1308
SacII CCGCGG 1 cut(s) 1365
SapI GCTCTTC 1 cut(s) 20
SaqAI TTAA 5 cut(s) 341, 378, 446, 936, 1691
SatI GCNGC 8 cut(s) 90, 208, 813, 1227, 1362, 1365, 1614, 1658
Sau3AI GATC 9 cut(s) 258, 502, 583, 921, 945, 1467, 1471, 1553, 1596
Sau96I GGNCC 4 cut(s) 574, 1061, 1308, 1547
ScaI AGTACT 1 cut(s) 911
SchI GAGTC 1 cut(s) 680
ScrFI CCNGG 2 cut(s) 1551, 1584
SduI GDGCHC 1 cut(s) 1517
SfaNI GCATC 4 cut(s) 400, 631, 790, 1600
SfcI CTRYAG 4 cut(s) 90, 1123, 1177, 1227
Sfr274I CTCGAG 1 cut(s) 314
Sfr303I CCGCGG 1 cut(s) 1365
SfuI TTCGAA 2 cut(s) 1048, 1090
SgrBI CCGCGG 1 cut(s) 1365
SinI GGWCC 4 cut(s) 574, 1061, 1308, 1547
SlaI CTCGAG 1 cut(s) 314
SmiMI CAYNNNNRTG 1 cut(s) 183
SmlI CTYRAG 2 cut(s) 314, 1299
SmoI CTYRAG 2 cut(s) 314, 1299
SnaBI TACGTA 1 cut(s) 886
Sse9I AATT 8 cut(s) 532, 735, 891, 1172, 1345, 1450, 1651, 1692
SsiI CCGC 8 cut(s) 119, 168, 208, 390, 975, 1306, 1362, 1364
SspMI CTAG 3 cut(s) 402, 834, 1563
StyD4I CCNGG 2 cut(s) 1549, 1582
StyI CCWWGG 2 cut(s) 1273, 1415
TaaI ACNGT 8 cut(s) 193, 376, 542, 1045, 1204, 1393, 1438, 1627
TaiI ACGT 3 cut(s) 464, 676, 888
TaqI TCGA 8 cut(s) 315, 614, 700, 899, 1048, 1090, 1430, 1470
TaqII GACCGA 1 cut(s) 1325
TasI AATT 8 cut(s) 532, 735, 891, 1172, 1345, 1450, 1651, 1692
TatI WGTACW 3 cut(s) 537, 909, 1533
TauI GCSGC 3 cut(s) 210, 1364, 1367
TfiI GAWTC 1 cut(s) 1318
Tru1I TTAA 5 cut(s) 341, 378, 446, 936, 1691
Tru9I TTAA 5 cut(s) 341, 378, 446, 936, 1691
TscAI CASTG 8 cut(s) 196, 547, 657, 868, 1060, 1396, 1515, 1687
TseFI GTSAC 2 cut(s) 648, 859
TseI GCWGC 5 cut(s) 89, 812, 1226, 1613, 1657
Tsp45I GTSAC 2 cut(s) 648, 859
TspDTI ATGAA 4 cut(s) 744, 1201, 1444, 1627
TspGWI ACGGA 2 cut(s) 505, 1212
TspRI CASTG 8 cut(s) 196, 547, 657, 868, 1060, 1396, 1515, 1687
Tth111I GACNNNGTC 1 cut(s) 419
VpaK11BI GGWCC 4 cut(s) 574, 1061, 1308, 1547
VspI ATTAAT 1 cut(s) 936
XagI CCTNNNNNAGG 1 cut(s) 1665
XapI RAATTY 3 cut(s) 532, 735, 1450
XcmI CCANNNNNNNNNTGG 3 cut(s) 773, 1006, 1639
XhoI CTCGAG 1 cut(s) 314
XspI CTAG 3 cut(s) 402, 834, 1563
ZrmI AGTACT 1 cut(s) 911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.