MD08G1195600.v1.1

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000522
Physical Location & Seq
Forward (+)
25225526 .. 25227571
2046 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1195600.v1.1.491

Sequence Viewer

Length: 1695 bp
ATGGCTTCTAAGTTCTTTCATCTTGTGAAAGTTTCATCATTTCTCATAATCTTCCATTTCCTCAGCTCTCGATCGCTAGCAGATGTCCCTCTAAACACTCCTCTCCCACCAGAAACCATTTGCAAGTCCACCCCACATCCTTCTTACTGCACATCTGTCCTTCCCCACAACAACGAAAGTGTCTATGACTTTGGCAGGTTTTCAGTCCAACGTGCACTTTCCGAATCCCATAAGCTCTTAGATTTGTATGAAAAATATCTTCAAAAGGGTTCAAGTTTAACAAATCCTGCAATCCAAGCACTTGAGGACTGCAAGCAGCTTGCATTGTTAAACATTGATTTCTTATCGAGCTCCCTTGAAACTGTTAACAAAGTGAGTGATGTTCTTCCTATCTTGGATGCCGATGATGTCCAAACATTGCTTAGCGCAATTCTAACCAACCACCAAACTTGTTCTGATGGGATTGCATCTCTGCCTTCTTCAGCTGGGAGTGTCCTAGGTGATCTATCAGTTCCACTCTCTAACAACACAAAATTGTTTAGTACCTCTTTGGCTCTCTTTACCAAGGGTTGGGTGCCTAAGGACAAAAATGGGGTGCCAAAGCAACCCAAGAGGCAGTTCAAATTCGGGAAGGGACGTTTGAACTTGAAAATGTCAAGCCATGCTCGTGCAATTTATGACTCCGCTATCAATCATCGGGGAAGAAGGCTACTTCAGGTGGGAGATGAAGAAGTTTTGGTGAAGGACATTGTGGTTGTGAGTCAAGATGGAAGTGGAAACTTCACCACCATCAATCAAGCCATTGCCATTGCACCAAATAACTCTGTTGCTAGTGGTGGGTACTTCATGATATATATCACTGCCGGTGTTTATGAAGAGTACGTGTCAATTATATCACAAAAGAAGTATTTGTTGTTCATCGGAGATGGTATCAATCAGACTATCATCACAGGAAATAACAGCGTAGGAGATGGATCGACAACTTTCAATTCTGCTACTTTAGCTGTGGTGGCACAAGGGTTTATGGCAGTAAACATCACTGTTCGTAACACAGCTGGGCCAAGCAAAGGACAAGCAGTTGCACTTCGAAGTGGAGCGGATTTTTCAGTGTTCTATAGTTGCAGCTTTGAAGGGTACCAAGATACCTTGTACACACATTCCCTCAGACAATTCTACAGAGAATGTGATATCTATGGAACAGTTGATTTCATATTCGGCAATGCTGCTGTTGTTCTACAAAATTGCAATATCTACCCTCGTCAGCCTAATCAAGGGCAATCCAATCCCATCACAGCTCAAGGTAGAACAGACCCGAATCAGAACACAGGAACTTCAATCCACAATTGCACTATTACACCTACACCTGATTTGGCTTCGAGTAATTATACTGTCAAGACCTATCTAGGGAGGCCATGGAAGGAGTATTCAAGAACAGTTTACATGCAAACTTTCATGGGAAGTTTAATTGATCCTGCTGGATGGCTTGCTTGGAGTGGAGACTTTGCACTAAGTACATTGTATTATGCTGAGTACAATAACACTGGTCCTGGATCAAGCACTACAAATAGAGTTACATGGCCCGGTTACCATGTGATCAATGCAACTGTTGCTGCTAATTTTACAGTCTCCAACTTCTTGCTGGGGGATAATTGGTTGCCTGACACTGGAGTCCCTTACACTGGTGGTTTGGTTTGA

Protein Analysis

565

Amino Acids

61.5

Weight (kDa)

6.82

Isoelectric Point (pI)

34.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 38 - 186 2.4e-22 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 251 - 549 2.9e-138 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000522)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02810 AT2G47550 AT4G02330
fragaria_vesca FvH4_2g25960 FvH4_2g25960 FvH4_2g25970 FvH4_5g35570 FvH4_5g35580 FvH4_5g35590
malus_domestica MD03G1290900.v1.1 MD03G1291000.v1.1 MD08G1195600.v1.1 MD11G1307400.v1.1 MD11G1307500.v1.1
prunus_persica Prupe.1G529400_v2.0.a1 Prupe.1G529500_v2.0.a1 Prupe.8G263900_v2.0.a1 Prupe.8G264000_v2.0.a1
pyrus_communis pycom08g16800
rosa_chinensis RchiOBHm_Chr6g0294321 RchiOBHm_Chr6g0294331 RchiOBHm_Chr7g0236951 RchiOBHm_Chr7g0236971 RchiOBHm_Chr7g0237041 RchiOBHm_Chr7g0237051
rosa_laevigata RLG00000001034 RLG00000001035 RLG00000001039 RLG00000001043 RLG00000001045 RLG00000011880 RLG00000011881 RLG00000034181
rosa_multiflora Rmu_co8086188.1_g000001 Rmu_co8458485.1_g000001 Rmu_sc0000272.1_g000007 Rmu_sc0000272.1_g000009 Rmu_sc0002131.1_g000030 Rmu_sc0002131.1_g000037 Rmu_sc0005369.1_g000005 Rmu_sc0005369.1_g000008 Rmu_sc0007991.1_g000005 Rmu_sc0007991.1_g000013 Rmu_sc0033563.1_g000001
rosa_roxburghii Rroxscaffold_3G00224540 Rroxscaffold_3G00224600 Rroxscaffold_3G00224650 Rroxscaffold_3G00224660 Rroxscaffold_7G00173470 Rroxscaffold_7G00173480
rosa_rugosa Rorug06G0243400 Rorug06G0243500 Rorug07G0301200 Rorug07G0301500 Rorug07G0301600
rosa_samantha Rh6AG356000 Rh6AG356100 Rh6BG362600 Rh6BG362800 Rh6CG370000 Rh6CG370100 Rh6DG356500 Rh6DG356600 Rh7AG456200 Rh7AG456500 Rh7AG457500 Rh7AG457600 Rh7BG427500 Rh7BG427700 Rh7BG428400 Rh7BG428500 Rh7CG475100 Rh7CG475300 Rh7CG475700 Rh7CG475800 Rh7DG444100 Rh7DG444300 Rh7DG444700 Rh7DG444900
rosa_wichuraiana Rw6G031020 Rw6G031030 Rw7G037870 Rw7G037880 Rw7G037920 Rw7G037940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1667
Acc36I ACCTGC 1 cut(s) 186
Acc65I GGTACC 1 cut(s) 1134
AccB1I GGYRCC 3 cut(s) 574, 595, 1134
AccB7I CCANNNNNTGG 1 cut(s) 570
AccBSI CCGCTC 1 cut(s) 1097
AciI CCGC 2 cut(s) 684, 1097
AclWI GGATC 3 cut(s) 982, 1463, 1558
AcsI RAATTY 1 cut(s) 623
AcuI CTGAAG 2 cut(s) 465, 698
AfaI GTAC 7 cut(s) 544, 842, 881, 1136, 1151, 1513, 1532
AfiI CCNNNNNNNGG 6 cut(s) 570, 1067, 1271, 1404, 1664, 1679
AflIII ACRYGT 1 cut(s) 882
AjnI CCWGG 1 cut(s) 1546
AjuI GAANNNNNNNTTGG 2 cut(s) 602, 634
AluBI AGCT 9 cut(s) 66, 235, 319, 351, 485, 1004, 1055, 1125, 1295
AluI AGCT 9 cut(s) 66, 235, 319, 351, 485, 1004, 1055, 1125, 1295
Alw21I GWGCWC 2 cut(s) 217, 353
Alw26I GTCTC 2 cut(s) 1491, 1630
Alw44I GTGCAC 1 cut(s) 213
AlwI GGATC 3 cut(s) 982, 1463, 1558
AoxI GGCC 3 cut(s) 1058, 1409, 1577
ApaLI GTGCAC 1 cut(s) 213
ApeKI GCWGC 4 cut(s) 316, 1122, 1223, 1610
ApoI RAATTY 1 cut(s) 623
Asp718I GGTACC 1 cut(s) 1134
AspA2I CCTAGG 1 cut(s) 496
AspLEI GCGC 1 cut(s) 428
AspS9I GGNCC 3 cut(s) 1058, 1544, 1578
AsuC2I CCSGG 1 cut(s) 1581
AsuHPI GGTGA 3 cut(s) 512, 751, 775
AsuII TTCGAA 1 cut(s) 1087
AsuNHI GCTAGC 1 cut(s) 76
AvaII GGWCC 1 cut(s) 1544
AvrII CCTAGG 1 cut(s) 496
AxyI CCTNAGG 1 cut(s) 579
BaeGI GKGCMC 1 cut(s) 217
BanI GGYRCC 3 cut(s) 574, 595, 1134
BanII GRGCYC 1 cut(s) 353
BauI CACGAG 1 cut(s) 666
Bbv12I GWGCWC 2 cut(s) 217, 353
BbvCI CCTCAGC 1 cut(s) 62
BbvI GCAGC 4 cut(s) 328, 1134, 1210, 1597
BccI CCATC 7 cut(s) 452, 761, 797, 920, 965, 1295, 1473
BciT130I CCWGG 1 cut(s) 1548
BclI TGATCA 1 cut(s) 1593
BcnI CCSGG 1 cut(s) 1581
BcoDI GTCTC 2 cut(s) 1491, 1630
BfaI CTAG 4 cut(s) 77, 497, 831, 1403
BfmI CTRYAG 2 cut(s) 1114, 1174
BfuAI ACCTGC 1 cut(s) 186
BisI GCNGC 4 cut(s) 317, 1123, 1224, 1611
BlnI CCTAGG 1 cut(s) 496
BlpI GCTNAGC 1 cut(s) 422
BlsI GCNGC 4 cut(s) 318, 1124, 1225, 1612
Bme1390I CCNGG 2 cut(s) 1548, 1581
Bme18I GGWCC 1 cut(s) 1544
BmgT120I GGNCC 3 cut(s) 1058, 1544, 1578
BmiI GGNNCC 3 cut(s) 576, 597, 1136
BmrFI CCNGG 2 cut(s) 1548, 1581
BmsI GCATC 2 cut(s) 388, 476
BmtI GCTAGC 1 cut(s) 80
BpmI CTGGAG 1 cut(s) 1686
Bpu10I CCTNAGC 1 cut(s) 62
Bpu1102I GCTNAGC 1 cut(s) 422
Bpu14I TTCGAA 1 cut(s) 1087
BpuEI CTTGAG 2 cut(s) 323, 1281
BpuMI CCSGG 1 cut(s) 1581
BsaAI YACGTR 1 cut(s) 883
BsaBI GATNNNNATC 1 cut(s) 854
BsaJI CCNNGG 3 cut(s) 496, 564, 1412
Bsc4I CCNNNNNNNGG 6 cut(s) 570, 1067, 1271, 1404, 1664, 1679
Bse118I RCCGGY 1 cut(s) 863
Bse1I ACTGG 3 cut(s) 1546, 1669, 1684
Bse21I CCTNAGG 1 cut(s) 579
Bse3DI GCAATG 4 cut(s) 416, 801, 807, 1225
Bse8I GATNNNNATC 1 cut(s) 854
BseBI CCWGG 1 cut(s) 1548
BseDI CCNNGG 3 cut(s) 496, 564, 1412
BseGI GGATG 3 cut(s) 136, 403, 1484
BseJI GATNNNNATC 1 cut(s) 854
BseLI CCNNNNNNNGG 6 cut(s) 570, 1067, 1271, 1404, 1664, 1679
BseMI GCAATG 4 cut(s) 416, 801, 807, 1225
BseMII CTCAG 3 cut(s) 76, 1177, 1518
BseNI ACTGG 3 cut(s) 1546, 1669, 1684
BseRI GAGGAG 1 cut(s) 90
BseSI GKGCMC 1 cut(s) 217
BseXI GCAGC 4 cut(s) 328, 1134, 1210, 1597
BseYI CCCAGC 3 cut(s) 485, 1055, 1639
BsgI GTGCAG 1 cut(s) 133
Bsh1285I CGRYCG 1 cut(s) 74
BshFI GGCC 3 cut(s) 1060, 1411, 1579
BshNI GGYRCC 3 cut(s) 574, 595, 1134
BsiEI CGRYCG 1 cut(s) 74
BsiHKAI GWGCWC 2 cut(s) 217, 353
BsiSI CCGG 2 cut(s) 864, 1581
BslFI GGGAC 3 cut(s) 71, 648, 1655
BslI CCNNNNNNNGG 6 cut(s) 570, 1067, 1271, 1404, 1664, 1679
BsmAI GTCTC 2 cut(s) 1491, 1630
BsmFI GGGAC 3 cut(s) 71, 648, 1655
BsnI GGCC 3 cut(s) 1060, 1411, 1579
Bsp119I TTCGAA 1 cut(s) 1087
Bsp1286I GDGCHC 2 cut(s) 217, 353
Bsp1407I TGTACA 1 cut(s) 1149
Bsp143I GATC 6 cut(s) 71, 502, 974, 1468, 1550, 1593
Bsp1720I GCTNAGC 1 cut(s) 422
Bsp19I CCATGG 1 cut(s) 1412
BspACI CCGC 2 cut(s) 684, 1097
BspANI GGCC 3 cut(s) 1060, 1411, 1579
BspCNI CTCAG 3 cut(s) 75, 1176, 1519
BspHI TCATGA 1 cut(s) 846
BspLI GGNNCC 3 cut(s) 576, 597, 1136
BspMI ACCTGC 1 cut(s) 186
BspOI GCTAGC 1 cut(s) 80
BspPI GGATC 3 cut(s) 982, 1463, 1558
BspT104I TTCGAA 1 cut(s) 1087
BspT107I GGYRCC 3 cut(s) 574, 595, 1134
BsrBI CCGCTC 1 cut(s) 1097
BsrDI GCAATG 4 cut(s) 416, 801, 807, 1225
BsrFI RCCGGY 1 cut(s) 863
BsrGI TGTACA 1 cut(s) 1149
BsrI ACTGG 3 cut(s) 1546, 1669, 1684
BssAI RCCGGY 1 cut(s) 863
BssECI CCNNGG 3 cut(s) 496, 564, 1412
BssMI GATC 6 cut(s) 71, 502, 974, 1468, 1550, 1593
BssSI CACGAG 1 cut(s) 666
BssT1I CCWWGG 3 cut(s) 496, 564, 1412
Bst2BI CACGAG 1 cut(s) 666
Bst2UI CCWGG 1 cut(s) 1548
Bst4CI ACNGT 7 cut(s) 364, 1042, 1201, 1390, 1435, 1606, 1624
Bst6I CTCTTC 1 cut(s) 870
BstAPI GCANNNNNTGC 1 cut(s) 1607
BstAUI TGTACA 1 cut(s) 1149
BstBAI YACGTR 1 cut(s) 883
BstBI TTCGAA 1 cut(s) 1087
BstC8I GCNNGC 4 cut(s) 78, 314, 321, 1485
BstDEI CTNAG 8 cut(s) 9, 62, 238, 422, 579, 1163, 1508, 1527
BstDSI CCRYGG 1 cut(s) 1412
BstEII GGTNACC 1 cut(s) 1583
BstENI CCTNNNNNAGG 1 cut(s) 1269
BstF5I GGATG 3 cut(s) 136, 403, 1484
BstHHI GCGC 1 cut(s) 428
BstKTI GATC 6 cut(s) 74, 505, 977, 1471, 1553, 1596
BstMAI GTCTC 2 cut(s) 1491, 1630
BstMBI GATC 6 cut(s) 71, 502, 974, 1468, 1550, 1593
BstMCI CGRYCG 1 cut(s) 74
BstMWI GCNNNNNNNGC 4 cut(s) 296, 1001, 1010, 1607
BstNI CCWGG 1 cut(s) 1548
BstNSI RCATGY 1 cut(s) 1444
BstPI GGTNACC 1 cut(s) 1583
BstSCI CCNGG 2 cut(s) 1546, 1579
BstSFI CTRYAG 2 cut(s) 1114, 1174
BstSLI GKGCMC 1 cut(s) 217
BstV1I GCAGC 4 cut(s) 328, 1134, 1210, 1597
Bsu36I CCTNAGG 1 cut(s) 579
BsuRI GGCC 3 cut(s) 1060, 1411, 1579
BtgI CCRYGG 1 cut(s) 1412
BtsCI GGATG 3 cut(s) 136, 403, 1484
BtsI GCAGTG 1 cut(s) 858
BtsIMutI CAGTG 6 cut(s) 858, 1038, 1113, 1539, 1662, 1677
BveI ACCTGC 1 cut(s) 186
Cac8I GCNNGC 4 cut(s) 78, 314, 321, 1485
CciI TCATGA 1 cut(s) 846
CfoI GCGC 1 cut(s) 428
Cfr10I RCCGGY 1 cut(s) 863
Cfr13I GGNCC 3 cut(s) 1058, 1544, 1578
Csp6I GTAC 7 cut(s) 543, 841, 880, 1135, 1150, 1512, 1531
CspCI CAANNNNNGTGG 2 cut(s) 431, 466
CviAII CATG 7 cut(s) 662, 847, 1413, 1441, 1453, 1575, 1589
CviQI GTAC 7 cut(s) 543, 841, 880, 1135, 1150, 1512, 1531
DdeI CTNAG 8 cut(s) 9, 62, 238, 422, 579, 1163, 1508, 1527
DpnI GATC 6 cut(s) 73, 504, 976, 1470, 1552, 1595
DpnII GATC 6 cut(s) 71, 502, 974, 1468, 1550, 1593
DrdI GACNNNNNNGTC 1 cut(s) 1667
DseDI GACNNNNNNGTC 1 cut(s) 1667
Eam1104I CTCTTC 1 cut(s) 870
EarI CTCTTC 1 cut(s) 870
Ecl136II GAGCTC 1 cut(s) 351
Eco130I CCWWGG 3 cut(s) 496, 564, 1412
Eco24I GRGCYC 1 cut(s) 353
Eco32I GATATC 1 cut(s) 1189
Eco47I GGWCC 1 cut(s) 1544
Eco53kI GAGCTC 1 cut(s) 351
Eco57I CTGAAG 2 cut(s) 465, 698
Eco81I CCTNAGG 1 cut(s) 579
Eco91I GGTNACC 1 cut(s) 1583
EcoICRI GAGCTC 1 cut(s) 351
EcoNI CCTNNNNNAGG 1 cut(s) 1269
EcoO65I GGTNACC 1 cut(s) 1583
EcoRII CCWGG 1 cut(s) 1546
EcoRV GATATC 1 cut(s) 1189
EcoT14I CCWWGG 3 cut(s) 496, 564, 1412
EcoT38I GRGCYC 1 cut(s) 353
ErhI CCWWGG 3 cut(s) 496, 564, 1412
FaeI CATG 7 cut(s) 665, 850, 1416, 1444, 1456, 1578, 1592
FaqI GGGAC 3 cut(s) 71, 648, 1655
FatI CATG 7 cut(s) 661, 846, 1412, 1440, 1452, 1574, 1588
FbaI TGATCA 1 cut(s) 1593
Fnu4HI GCNGC 4 cut(s) 317, 1123, 1224, 1611
FokI GGATG 3 cut(s) 123, 410, 1491
FriOI GRGCYC 1 cut(s) 353
Fsp4HI GCNGC 4 cut(s) 317, 1123, 1224, 1611
FspBI CTAG 4 cut(s) 77, 497, 831, 1403
GlaI GCGC 1 cut(s) 427
GluI GCNGC 4 cut(s) 317, 1123, 1224, 1611
GsaI CCCAGC 3 cut(s) 489, 1059, 1643
GsuI CTGGAG 1 cut(s) 1686
HaeIII GGCC 3 cut(s) 1060, 1411, 1579
HapII CCGG 2 cut(s) 864, 1581
HhaI GCGC 1 cut(s) 428
Hin1II CATG 7 cut(s) 665, 850, 1416, 1444, 1456, 1578, 1592
Hin6I GCGC 1 cut(s) 426
HinP1I GCGC 1 cut(s) 426
HincII GTYRAC 1 cut(s) 367
HindII GTYRAC 1 cut(s) 367
HinfI GANTC 5 cut(s) 224, 680, 760, 1315, 1668
HpaI GTTAAC 1 cut(s) 367
HpaII CCGG 2 cut(s) 864, 1581
HphI GGTGA 3 cut(s) 512, 751, 775
Hpy166II GTNNAC 6 cut(s) 129, 215, 367, 1033, 1152, 1438
Hpy188I TCNGA 6 cut(s) 223, 457, 923, 939, 1166, 1320
Hpy188III TCNNGA 6 cut(s) 69, 628, 764, 847, 1393, 1428
Hpy8I GTNNAC 6 cut(s) 129, 215, 367, 1033, 1152, 1438
HpyAV CCTTC 8 cut(s) 150, 170, 486, 625, 699, 736, 1124, 1411
HpyCH4III ACNGT 7 cut(s) 364, 1042, 1201, 1390, 1435, 1606, 1624
HpyCH4IV ACGT 3 cut(s) 211, 637, 882
HpyF10VI GCNNNNNNNGC 4 cut(s) 296, 1001, 1010, 1607
HpyF3I CTNAG 8 cut(s) 9, 62, 238, 422, 579, 1163, 1508, 1527
HpySE526I ACGT 3 cut(s) 211, 637, 882
Hsp92II CATG 7 cut(s) 665, 850, 1416, 1444, 1456, 1578, 1592
HspAI GCGC 1 cut(s) 426
KpnI GGTACC 1 cut(s) 1138
Ksp22I TGATCA 1 cut(s) 1593
KspAI GTTAAC 1 cut(s) 367
Kzo9I GATC 6 cut(s) 71, 502, 974, 1468, 1550, 1593
LmnI GCTCC 2 cut(s) 356, 1094
Lsp1109I GCAGC 4 cut(s) 328, 1134, 1210, 1597
LweI GCATC 2 cut(s) 388, 476
MaeI CTAG 4 cut(s) 77, 497, 831, 1403
MaeII ACGT 3 cut(s) 211, 637, 882
MaeIII GTNAC 3 cut(s) 1046, 1570, 1583
MalI GATC 6 cut(s) 73, 504, 976, 1470, 1552, 1595
MbiI CCGCTC 1 cut(s) 1097
MboI GATC 6 cut(s) 71, 502, 974, 1468, 1550, 1593
MboII GAAGA 7 cut(s) 43, 251, 377, 471, 714, 740, 887
MfeI CAATTG 1 cut(s) 1342
MhlI GDGCHC 2 cut(s) 217, 353
MlyI GAGTC 3 cut(s) 674, 769, 1677
MmeI TCCRAC 2 cut(s) 232, 1653
MnlI CCTC 9 cut(s) 71, 99, 111, 298, 556, 606, 1172, 1266, 1401
MseI TTAA 4 cut(s) 278, 329, 366, 1463
MslI CAYNNNNRTG 1 cut(s) 666
MspA1I CMGCKG 2 cut(s) 485, 1055
MspI CCGG 2 cut(s) 864, 1581
MspR9I CCNGG 2 cut(s) 1548, 1581
MunI CAATTG 1 cut(s) 1342
MvaI CCWGG 1 cut(s) 1548
MwoI GCNNNNNNNGC 4 cut(s) 296, 1001, 1010, 1607
NciI CCSGG 1 cut(s) 1581
NcoI CCATGG 1 cut(s) 1412
NdeII GATC 6 cut(s) 71, 502, 974, 1468, 1550, 1593
NheI GCTAGC 1 cut(s) 76
NlaIII CATG 7 cut(s) 665, 850, 1416, 1444, 1456, 1578, 1592
NlaIV GGNNCC 3 cut(s) 576, 597, 1136
NspI RCATGY 1 cut(s) 1444
NspV TTCGAA 1 cut(s) 1087
PagI TCATGA 1 cut(s) 846
PfeI GAWTC 2 cut(s) 224, 1315
PflMI CCANNNNNTGG 1 cut(s) 570
PfoI TCCNGGA 1 cut(s) 1546
PkrI GCNGC 4 cut(s) 318, 1124, 1225, 1612
Ple19I CGATCG 1 cut(s) 74
PleI GAGTC 3 cut(s) 674, 768, 1676
PpsI GAGTC 3 cut(s) 674, 768, 1676
Ppu21I YACGTR 1 cut(s) 883
Psp124BI GAGCTC 1 cut(s) 353
Psp6I CCWGG 1 cut(s) 1546
PspEI GGTNACC 1 cut(s) 1583
PspFI CCCAGC 3 cut(s) 485, 1055, 1639
PspGI CCWGG 1 cut(s) 1546
PspN4I GGNNCC 3 cut(s) 576, 597, 1136
PspPI GGNCC 3 cut(s) 1058, 1544, 1578
PsrI GAACNNNNNNTAC 2 cut(s) 899, 931
PvuI CGATCG 1 cut(s) 74
PvuII CAGCTG 2 cut(s) 485, 1055
RsaI GTAC 7 cut(s) 544, 842, 881, 1136, 1151, 1513, 1532
RsaNI GTAC 7 cut(s) 543, 841, 880, 1135, 1150, 1512, 1531
RseI CAYNNNNRTG 1 cut(s) 666
SacI GAGCTC 1 cut(s) 353
SaqAI TTAA 4 cut(s) 278, 329, 366, 1463
SatI GCNGC 4 cut(s) 317, 1123, 1224, 1611
Sau3AI GATC 6 cut(s) 71, 502, 974, 1468, 1550, 1593
Sau96I GGNCC 3 cut(s) 1058, 1544, 1578
SchI GAGTC 3 cut(s) 674, 769, 1677
ScrFI CCNGG 2 cut(s) 1548, 1581
SduI GDGCHC 2 cut(s) 217, 353
SfaNI GCATC 2 cut(s) 388, 476
SfcI CTRYAG 2 cut(s) 1114, 1174
SfuI TTCGAA 1 cut(s) 1087
SinI GGWCC 1 cut(s) 1544
SmiMI CAYNNNNRTG 1 cut(s) 666
SmlI CTYRAG 2 cut(s) 302, 1296
SmoI CTYRAG 2 cut(s) 302, 1296
SsiI CCGC 2 cut(s) 684, 1097
SspMI CTAG 4 cut(s) 77, 497, 831, 1403
SstI GAGCTC 1 cut(s) 353
StyD4I CCNGG 2 cut(s) 1546, 1579
StyI CCWWGG 3 cut(s) 496, 564, 1412
TaaI ACNGT 7 cut(s) 364, 1042, 1201, 1390, 1435, 1606, 1624
TaiI ACGT 3 cut(s) 214, 640, 885
TaqI TCGA 5 cut(s) 70, 347, 977, 1087, 1376
TatI WGTACW 3 cut(s) 1149, 1511, 1530
TfiI GAWTC 2 cut(s) 224, 1315
Tru1I TTAA 4 cut(s) 278, 329, 366, 1463
Tru9I TTAA 4 cut(s) 278, 329, 366, 1463
TscAI CASTG 6 cut(s) 865, 1045, 1113, 1546, 1669, 1684
TseI GCWGC 4 cut(s) 316, 1122, 1223, 1610
TspDTI ATGAA 9 cut(s) 8, 24, 264, 741, 835, 888, 907, 1198, 1441
TspRI CASTG 6 cut(s) 865, 1045, 1113, 1546, 1669, 1684
Van91I CCANNNNNTGG 1 cut(s) 570
VneI GTGCAC 1 cut(s) 213
VpaK11BI GGWCC 1 cut(s) 1544
XagI CCTNNNNNAGG 1 cut(s) 1269
XapI RAATTY 1 cut(s) 623
XceI RCATGY 1 cut(s) 1444
XcmI CCANNNNNNNNNTGG 1 cut(s) 1636
XmaJI CCTAGG 1 cut(s) 496
XspI CTAG 4 cut(s) 77, 497, 831, 1403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.