FvH4_2g25970

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000522
Physical Location & Seq
Reverse (-)
20930867 .. 20932837
1971 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g25970.t1

Sequence Viewer

Length: 1638 bp
ATGGATTCACAACCATTCTTTGTGTTCAAACTATCAATCGTCAGTCTTCATCTTATTCCTATCCTTTTTGCCTCTGGATGTTTAGCTTCAGAAGCAGGTTGTGCAACTGATACAGTACCCAAAGAAATCATTTGCAAGAACACCCGAAACCCTTCATATTGCAACTCCATTCTCCCCAACCAAACCACCAATCTATATGACCACGGCCGTTTTGCGGTCCGGAAATCATTATCCCAATCCCATAAATTGCATCACTTGGTGAACAAGTATCTTAAAGCCAACTCGAATCTCTCCAAAACCGCAATCCTCGCCCTTGAAGATTGCAAGCTTCTTGCGGAACTCAACGTAGATTTTCTTGCAAAATCCCTAAAAAACCTCAACGACACTAACCAAACACTTCCCAGCTCGAAAGCTGAGGACGTCCATACATGGCTCAGTGCCGTCCTCACAAACCAGCAAACATGCTTGGATAGCGTACAGGCAACTTCTTCTTCATCATCTTGGAGGAGTATGCAAAATGGACTTTTGCTCTCCGACAACACGAACCTTTATAGTGTTTCTCTAGCTCTGTTCACAAAGGGATGGGTGCCTGAAACGGAGGAGGAGGAGGTGGTCGAGAAGGAGGAGGAGGTGATCACATGGAGGGAACAGCTGCTTCGGAATTTTGGTGACGTTTTGGTGAGTGAGACTGTCACCGTGAGTCAGGACGGAAATGGGAACTTCACCACCGTAAACGAAGCTGTTGCCGCAGCTCCGATCAACACTGAAGGTAAAAATGGTTACTTCTTGATTTATATCAAGGCAGGAGTTTATGAGGAGTATGTGAACATTGCAAAAAACAAAAAGTATCTGATGATGATCGGTGATGGCATCAACCGGACTATCATAACAGGCAATAGGAGTGTTGTAGATGGCTGGACAACTTTCAATTCAGCAACATTTGTGGTAATGGCACCATTGTTTGTAGCAGTGAACATGACCTTCCGCAACACCGCCGGAGCAAGCAAACACCAAGCCGTCGCCGTTCGAAATGATGGCGATCTATCCGCATTCTACCATTGCAGCTTCGAAGGGTACCAAGACACCTTATACACACATTCACAAAGACAATTCTACAAGCAGTGCGACGTCTACGGAACAGTGGACTTCATATTCGGAAACGCAGCAGCTATTTTACAAAACTGTAACATCTACCCTCGGCTTCCGATGCGCGGCCAGTTCAACGCCATCACTGCTCAGGGTCGATCCGACCCGAATCAAAACACAGGCATTATGATCCACAACTGTAACATCAGAGCGTCCGATGACCTAGCTTCGGACAAGGGAAAAACGAGGACTTATCTAGGGAGGCCATGGAGGGAGTATTCGAGGACGGTGTACATGAAATGCTTCATGGATGGTTGCATAGACAGGGAAGGTTGGAGTAAATGGACGGGAGATTTTGCTCTAAGCACATTGTATTACGCCGAGTATAAATGTAAGGGCCCTGGATCGAAAACTAGGGGAAGAGTTACATGGCGAGGTTACCATATATTGAGAAAACCTAAGGAAGCTAAAAATTTTACAGTGTCCAAGTTCTTGCGTGGAAGGAAGTGGCTGCCTCAAACTGGAGTGCCATTTACCAGTGGATTGATTTAG

Protein Analysis

546

Amino Acids

61.1

Weight (kDa)

8.78

Isoelectric Point (pI)

32.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 44 - 190 2.8e-22 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 231 - 529 3e-137 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000522)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02810 AT2G47550 AT4G02330
fragaria_vesca FvH4_2g25960 FvH4_2g25960 FvH4_2g25970 FvH4_5g35570 FvH4_5g35580 FvH4_5g35590
malus_domestica MD03G1290900.v1.1 MD03G1291000.v1.1 MD08G1195600.v1.1 MD11G1307400.v1.1 MD11G1307500.v1.1
prunus_persica Prupe.1G529400_v2.0.a1 Prupe.1G529500_v2.0.a1 Prupe.8G263900_v2.0.a1 Prupe.8G264000_v2.0.a1
pyrus_communis pycom08g16800
rosa_chinensis RchiOBHm_Chr6g0294321 RchiOBHm_Chr6g0294331 RchiOBHm_Chr7g0236951 RchiOBHm_Chr7g0236971 RchiOBHm_Chr7g0237041 RchiOBHm_Chr7g0237051
rosa_laevigata RLG00000001034 RLG00000001035 RLG00000001039 RLG00000001043 RLG00000001045 RLG00000011880 RLG00000011881 RLG00000034181
rosa_multiflora Rmu_co8086188.1_g000001 Rmu_co8458485.1_g000001 Rmu_sc0000272.1_g000007 Rmu_sc0000272.1_g000009 Rmu_sc0002131.1_g000030 Rmu_sc0002131.1_g000037 Rmu_sc0005369.1_g000005 Rmu_sc0005369.1_g000008 Rmu_sc0007991.1_g000005 Rmu_sc0007991.1_g000013 Rmu_sc0033563.1_g000001
rosa_roxburghii Rroxscaffold_3G00224540 Rroxscaffold_3G00224600 Rroxscaffold_3G00224650 Rroxscaffold_3G00224660 Rroxscaffold_7G00173470 Rroxscaffold_7G00173480
rosa_rugosa Rorug06G0243400 Rorug06G0243500 Rorug07G0301200 Rorug07G0301500 Rorug07G0301600
rosa_samantha Rh6AG356000 Rh6AG356100 Rh6BG362600 Rh6BG362800 Rh6CG370000 Rh6CG370100 Rh6DG356500 Rh6DG356600 Rh7AG456200 Rh7AG456500 Rh7AG457500 Rh7AG457600 Rh7BG427500 Rh7BG427700 Rh7BG428400 Rh7BG428500 Rh7CG475100 Rh7CG475300 Rh7CG475700 Rh7CG475800 Rh7DG444100 Rh7DG444300 Rh7DG444700 Rh7DG444900
rosa_wichuraiana Rw6G031020 Rw6G031030 Rw7G037870 Rw7G037880 Rw7G037920 Rw7G037940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 423, 1131
Acc36I ACCTGC 1 cut(s) 86
Acc65I GGTACC 1 cut(s) 1074
AccB1I GGYRCC 3 cut(s) 586, 952, 1074
AccI GTMKAC 1 cut(s) 1131
AccII CGCG 1 cut(s) 1212
AccIII TCCGGA 1 cut(s) 219
AciI CCGC 8 cut(s) 215, 300, 335, 747, 985, 993, 1047, 1212
AclWI GGATC 3 cut(s) 1239, 1270, 1498
AcoI YGGCCR 2 cut(s) 205, 1213
AcsI RAATTY 2 cut(s) 661, 1558
AcuI CTGAAG 2 cut(s) 72, 786
AcyI GRCGYC 2 cut(s) 420, 1128
AdeI CACNNNGTG 1 cut(s) 259
AfaI GTAC 4 cut(s) 117, 477, 1076, 1379
AfiI CCNNNNNNNGG 4 cut(s) 214, 1211, 1315, 1607
AgsI TTSAA 4 cut(s) 28, 317, 928, 1222
AjnI CCWGG 1 cut(s) 1486
Alw26I GTCTC 1 cut(s) 680
AlwI GGATC 3 cut(s) 1239, 1270, 1498
Aor13HI TCCGGA 1 cut(s) 219
AoxI GGCC 4 cut(s) 205, 1213, 1349, 1483
ApaI GGGCCC 1 cut(s) 1487
ApeKI GCWGC 6 cut(s) 652, 749, 1062, 1163, 1166, 1597
ApoI RAATTY 2 cut(s) 661, 1558
ArsI GACNNNNNNTTYG 2 cut(s) 1136, 1168
Asp700I GAANNNNTTC 2 cut(s) 151, 1388
Asp718I GGTACC 1 cut(s) 1074
AspLEI GCGC 1 cut(s) 1212
AspS9I GGNCC 3 cut(s) 217, 1483, 1484
AsuHPI GGTGA 7 cut(s) 271, 643, 680, 685, 691, 715, 875
AsuII TTCGAA 2 cut(s) 1027, 1068
AvaII GGWCC 1 cut(s) 217
AxyI CCTNAGG 1 cut(s) 1545
BaeGI GKGCMC 1 cut(s) 1487
BanI GGYRCC 3 cut(s) 586, 952, 1074
BanII GRGCYC 1 cut(s) 1487
BbsI GAAGAC 1 cut(s) 38
BbvCI CCTCAGC 1 cut(s) 414
BbvI GCAGC 6 cut(s) 639, 761, 1074, 1175, 1178, 1584
BccI CCATC 6 cut(s) 576, 860, 905, 1028, 1235, 1391
BceAI ACGGC 5 cut(s) 192, 220, 425, 1001, 1007
BcgI CGANNNNNNTGC 2 cut(s) 725, 759
BciT130I CCWGG 1 cut(s) 1488
BclI TGATCA 1 cut(s) 633
BcoDI GTCTC 1 cut(s) 680
BfaI CTAG 4 cut(s) 563, 1310, 1343, 1500
BfuAI ACCTGC 1 cut(s) 86
BisI GCNGC 8 cut(s) 653, 747, 750, 1063, 1164, 1167, 1213, 1598
BlsI GCNGC 8 cut(s) 654, 748, 751, 1064, 1165, 1168, 1214, 1599
Bme1390I CCNGG 1 cut(s) 1488
Bme18I GGWCC 1 cut(s) 217
BmgT120I GGNCC 3 cut(s) 217, 1483, 1484
BmiI GGNNCC 4 cut(s) 588, 954, 1076, 1485
BmrFI CCNGG 1 cut(s) 1488
BmsI GCATC 3 cut(s) 259, 879, 1197
BpiI GAAGAC 1 cut(s) 38
BpmI CTGGAG 1 cut(s) 1629
Bpu10I CCTNAGC 2 cut(s) 414, 1236
Bpu14I TTCGAA 2 cut(s) 1027, 1068
BsaBI GATNNNNATC 3 cut(s) 794, 857, 1038
BsaHI GRCGYC 2 cut(s) 420, 1128
BsaJI CCNNGG 4 cut(s) 202, 1196, 1352, 1486
BsaWI WCCGGW 2 cut(s) 219, 876
Bsc4I CCNNNNNNNGG 4 cut(s) 214, 1211, 1315, 1607
Bse1I ACTGG 3 cut(s) 1216, 1612, 1623
Bse21I CCTNAGG 1 cut(s) 1545
Bse3DI GCAATG 2 cut(s) 828, 1057
Bse8I GATNNNNATC 3 cut(s) 794, 857, 1038
BseAI TCCGGA 1 cut(s) 219
BseBI CCWGG 1 cut(s) 1488
BseDI CCNNGG 4 cut(s) 202, 1196, 1352, 1486
BseGI GGATG 3 cut(s) 83, 587, 1402
BseJI GATNNNNATC 3 cut(s) 794, 857, 1038
BseLI CCNNNNNNNGG 4 cut(s) 214, 1211, 1315, 1607
BseMI GCAATG 2 cut(s) 828, 1057
BseMII CTCAG 3 cut(s) 405, 448, 1250
BseNI ACTGG 3 cut(s) 1216, 1612, 1623
BseRI GAGGAG 7 cut(s) 520, 614, 617, 620, 638, 641, 830
BseSI GKGCMC 1 cut(s) 1487
BseX3I CGGCCG 1 cut(s) 205
BseXI GCAGC 6 cut(s) 639, 761, 1074, 1175, 1178, 1584
BseYI CCCAGC 1 cut(s) 401
Bsh1236I CGCG 1 cut(s) 1212
Bsh1285I CGRYCG 1 cut(s) 208
BshFI GGCC 4 cut(s) 207, 1215, 1351, 1485
BshNI GGYRCC 3 cut(s) 586, 952, 1074
BsiEI CGRYCG 1 cut(s) 208
BsiSI CCGG 3 cut(s) 220, 877, 996
BslI CCNNNNNNNGG 4 cut(s) 214, 1211, 1315, 1607
BsmAI GTCTC 1 cut(s) 680
BsmI GAATGC 1 cut(s) 1049
BsnI GGCC 4 cut(s) 207, 1215, 1351, 1485
Bsp119I TTCGAA 2 cut(s) 1027, 1068
Bsp120I GGGCCC 1 cut(s) 1483
Bsp1286I GDGCHC 1 cut(s) 1487
Bsp13I TCCGGA 1 cut(s) 219
Bsp1407I TGTACA 1 cut(s) 1377
Bsp143I GATC 7 cut(s) 633, 756, 858, 1039, 1244, 1275, 1490
Bsp19I CCATGG 1 cut(s) 1352
BspACI CCGC 8 cut(s) 215, 300, 335, 747, 985, 993, 1047, 1212
BspANI GGCC 4 cut(s) 207, 1215, 1351, 1485
BspCNI CTCAG 3 cut(s) 406, 447, 1249
BspEI TCCGGA 1 cut(s) 219
BspFNI CGCG 1 cut(s) 1212
BspLI GGNNCC 4 cut(s) 588, 954, 1076, 1485
BspMI ACCTGC 1 cut(s) 86
BspPI GGATC 3 cut(s) 1239, 1270, 1498
BspT104I TTCGAA 2 cut(s) 1027, 1068
BspT107I GGYRCC 3 cut(s) 586, 952, 1074
BsrDI GCAATG 2 cut(s) 828, 1057
BsrGI TGTACA 1 cut(s) 1377
BsrI ACTGG 3 cut(s) 1216, 1612, 1623
BssECI CCNNGG 4 cut(s) 202, 1196, 1352, 1486
BssMI GATC 7 cut(s) 633, 756, 858, 1039, 1244, 1275, 1490
BssNI GRCGYC 2 cut(s) 420, 1128
BssT1I CCWWGG 1 cut(s) 1352
Bst2UI CCWGG 1 cut(s) 1488
Bst4CI ACNGT 9 cut(s) 115, 691, 697, 730, 1141, 1184, 1286, 1375, 1567
Bst6I CTCTTC 1 cut(s) 1501
BstACI GRCGYC 2 cut(s) 420, 1128
BstAPI GCANNNNNTGC 1 cut(s) 101
BstAUI TGTACA 1 cut(s) 1377
BstBI TTCGAA 2 cut(s) 1027, 1068
BstC8I GCNNGC 2 cut(s) 326, 1003
BstDEI CTNAG 5 cut(s) 414, 434, 1236, 1448, 1545
BstDSI CCRYGG 2 cut(s) 202, 1352
BstEII GGTNACC 1 cut(s) 1523
BstF5I GGATG 3 cut(s) 83, 587, 1402
BstFNI CGCG 1 cut(s) 1212
BstHHI GCGC 1 cut(s) 1212
BstKTI GATC 7 cut(s) 636, 759, 861, 1042, 1247, 1278, 1493
BstMAI GTCTC 1 cut(s) 680
BstMBI GATC 7 cut(s) 633, 756, 858, 1039, 1244, 1275, 1490
BstMCI CGRYCG 1 cut(s) 208
BstMWI GCNNNNNNNGC 7 cut(s) 92, 101, 308, 471, 746, 1207, 1232
BstNI CCWGG 1 cut(s) 1488
BstNSI RCATGY 1 cut(s) 465
BstPI GGTNACC 1 cut(s) 1523
BstSCI CCNGG 1 cut(s) 1486
BstSLI GKGCMC 1 cut(s) 1487
BstUI CGCG 1 cut(s) 1212
BstV1I GCAGC 6 cut(s) 639, 761, 1074, 1175, 1178, 1584
BstV2I GAAGAC 1 cut(s) 38
BstZI CGGCCG 1 cut(s) 205
Bsu36I CCTNAGG 1 cut(s) 1545
BsuRI GGCC 4 cut(s) 207, 1215, 1351, 1485
BtgI CCRYGG 2 cut(s) 202, 1352
BtsCI GGATG 3 cut(s) 83, 587, 1402
BtsI GCAGTG 3 cut(s) 975, 1127, 1230
BtsIMutI CAGTG 8 cut(s) 442, 762, 975, 1127, 1146, 1230, 1572, 1630
BveI ACCTGC 1 cut(s) 86
Cac8I GCNNGC 2 cut(s) 326, 1003
CfoI GCGC 1 cut(s) 1212
Cfr13I GGNCC 3 cut(s) 217, 1483, 1484
CpoI CGGWCCG 1 cut(s) 217
CseI GACGC 1 cut(s) 1287
Csp6I GTAC 4 cut(s) 116, 476, 1075, 1378
CspCI CAANNNNNGTGG 2 cut(s) 924, 959
CspI CGGWCCG 1 cut(s) 217
CviAII CATG 8 cut(s) 429, 462, 639, 976, 1353, 1381, 1393, 1515
CviQI GTAC 4 cut(s) 116, 476, 1075, 1378
DdeI CTNAG 5 cut(s) 414, 434, 1236, 1448, 1545
DpnI GATC 7 cut(s) 635, 758, 860, 1041, 1246, 1277, 1492
DpnII GATC 7 cut(s) 633, 756, 858, 1039, 1244, 1275, 1490
DraIII CACNNNGTG 1 cut(s) 259
EaeI YGGCCR 2 cut(s) 205, 1213
EagI CGGCCG 1 cut(s) 205
Eam1104I CTCTTC 1 cut(s) 1501
EarI CTCTTC 1 cut(s) 1501
EclXI CGGCCG 1 cut(s) 205
Eco130I CCWWGG 1 cut(s) 1352
Eco24I GRGCYC 1 cut(s) 1487
Eco47I GGWCC 1 cut(s) 217
Eco52I CGGCCG 1 cut(s) 205
Eco57I CTGAAG 2 cut(s) 72, 786
Eco81I CCTNAGG 1 cut(s) 1545
Eco91I GGTNACC 1 cut(s) 1523
EcoO109I RGGNCCY 2 cut(s) 1483, 1484
EcoO65I GGTNACC 1 cut(s) 1523
EcoRII CCWGG 1 cut(s) 1486
EcoT14I CCWWGG 1 cut(s) 1352
EcoT38I GRGCYC 1 cut(s) 1487
ErhI CCWWGG 1 cut(s) 1352
FaeI CATG 8 cut(s) 432, 465, 642, 979, 1356, 1384, 1396, 1518
FatI CATG 8 cut(s) 428, 461, 638, 975, 1352, 1380, 1392, 1514
FbaI TGATCA 1 cut(s) 633
FblI GTMKAC 1 cut(s) 1131
Fnu4HI GCNGC 8 cut(s) 653, 747, 750, 1063, 1164, 1167, 1213, 1598
FokI GGATG 3 cut(s) 90, 594, 1409
FriOI GRGCYC 1 cut(s) 1487
Fsp4HI GCNGC 8 cut(s) 653, 747, 750, 1063, 1164, 1167, 1213, 1598
FspBI CTAG 4 cut(s) 563, 1310, 1343, 1500
GlaI GCGC 1 cut(s) 1211
GluI GCNGC 8 cut(s) 653, 747, 750, 1063, 1164, 1167, 1213, 1598
GsaI CCCAGC 1 cut(s) 405
GsuI CTGGAG 1 cut(s) 1629
HaeIII GGCC 4 cut(s) 207, 1215, 1351, 1485
HapII CCGG 3 cut(s) 220, 877, 996
HgaI GACGC 1 cut(s) 1287
HhaI GCGC 1 cut(s) 1212
Hin1I GRCGYC 2 cut(s) 420, 1128
Hin1II CATG 8 cut(s) 432, 465, 642, 979, 1356, 1384, 1396, 1518
Hin6I GCGC 1 cut(s) 1210
HinP1I GCGC 1 cut(s) 1210
HindIII AAGCTT 1 cut(s) 326
HinfI GANTC 4 cut(s) 5, 286, 700, 1255
HpaII CCGG 3 cut(s) 220, 877, 996
HphI GGTGA 7 cut(s) 271, 643, 680, 685, 691, 715, 875
Hpy166II GTNNAC 8 cut(s) 262, 573, 733, 826, 973, 1132, 1144, 1378
Hpy188III TCNNGA 5 cut(s) 75, 220, 616, 704, 787
Hpy8I GTNNAC 8 cut(s) 262, 573, 733, 826, 973, 1132, 1144, 1378
Hpy99I CGWCG 2 cut(s) 1022, 1130
HpyAV CCTTC 7 cut(s) 162, 613, 761, 991, 1064, 1409, 1581
HpyCH4III ACNGT 9 cut(s) 115, 691, 697, 730, 1141, 1184, 1286, 1375, 1567
HpyCH4IV ACGT 4 cut(s) 345, 420, 672, 1128
HpyF10VI GCNNNNNNNGC 7 cut(s) 92, 101, 308, 471, 746, 1207, 1232
HpyF3I CTNAG 5 cut(s) 414, 434, 1236, 1448, 1545
HpySE526I ACGT 4 cut(s) 345, 420, 672, 1128
Hsp92I GRCGYC 2 cut(s) 420, 1128
Hsp92II CATG 8 cut(s) 432, 465, 642, 979, 1356, 1384, 1396, 1518
HspAI GCGC 1 cut(s) 1210
Kpn2I TCCGGA 1 cut(s) 219
KpnI GGTACC 1 cut(s) 1078
Ksp22I TGATCA 1 cut(s) 633
Kzo9I GATC 7 cut(s) 633, 756, 858, 1039, 1244, 1275, 1490
LmnI GCTCC 2 cut(s) 757, 998
Lsp1109I GCAGC 6 cut(s) 639, 761, 1074, 1175, 1178, 1584
LweI GCATC 3 cut(s) 259, 879, 1197
MaeI CTAG 4 cut(s) 563, 1310, 1343, 1500
MaeII ACGT 4 cut(s) 345, 420, 672, 1128
MaeIII GTNAC 7 cut(s) 668, 691, 779, 1184, 1286, 1510, 1523
MalI GATC 7 cut(s) 635, 758, 860, 1041, 1246, 1277, 1492
MboI GATC 7 cut(s) 633, 756, 858, 1039, 1244, 1275, 1490
MboII GAAGA 5 cut(s) 38, 329, 480, 483, 1518
MhlI GDGCHC 1 cut(s) 1487
MluCI AATT 5 cut(s) 245, 661, 928, 1109, 1558
MlyI GAGTC 1 cut(s) 709
MmeI TCCRAC 3 cut(s) 558, 1272, 1400
MroI TCCGGA 1 cut(s) 219
MroXI GAANNNNTTC 2 cut(s) 151, 1388
MseI TTAA 1 cut(s) 273
MspA1I CMGCKG 1 cut(s) 652
MspI CCGG 3 cut(s) 220, 877, 996
MspR9I CCNGG 1 cut(s) 1488
Mva1269I GAATGC 1 cut(s) 1049
MvaI CCWGG 1 cut(s) 1488
MvnI CGCG 1 cut(s) 1212
MwoI GCNNNNNNNGC 7 cut(s) 92, 101, 308, 471, 746, 1207, 1232
NcoI CCATGG 1 cut(s) 1352
NdeII GATC 7 cut(s) 633, 756, 858, 1039, 1244, 1275, 1490
NlaIII CATG 8 cut(s) 432, 465, 642, 979, 1356, 1384, 1396, 1518
NlaIV GGNNCC 4 cut(s) 588, 954, 1076, 1485
NmeAIII GCCGAG 2 cut(s) 1177, 1492
NmuCI GTSAC 2 cut(s) 668, 691
NspI RCATGY 1 cut(s) 465
NspV TTCGAA 2 cut(s) 1027, 1068
PctI GAATGC 1 cut(s) 1049
PdmI GAANNNNTTC 2 cut(s) 151, 1388
PfeI GAWTC 3 cut(s) 5, 286, 1255
PkrI GCNGC 8 cut(s) 654, 748, 751, 1064, 1165, 1168, 1214, 1599
PleI GAGTC 1 cut(s) 708
PpsI GAGTC 1 cut(s) 708
Psp6I CCWGG 1 cut(s) 1486
PspEI GGTNACC 1 cut(s) 1523
PspFI CCCAGC 1 cut(s) 401
PspGI CCWGG 1 cut(s) 1486
PspN4I GGNNCC 4 cut(s) 588, 954, 1076, 1485
PspOMI GGGCCC 1 cut(s) 1483
PspPI GGNCC 3 cut(s) 217, 1483, 1484
PvuII CAGCTG 1 cut(s) 652
RsaI GTAC 4 cut(s) 117, 477, 1076, 1379
RsaNI GTAC 4 cut(s) 116, 476, 1075, 1378
Rsr2I CGGWCCG 1 cut(s) 217
RsrII CGGWCCG 1 cut(s) 217
SaqAI TTAA 1 cut(s) 273
SatI GCNGC 8 cut(s) 653, 747, 750, 1063, 1164, 1167, 1213, 1598
Sau3AI GATC 7 cut(s) 633, 756, 858, 1039, 1244, 1275, 1490
Sau96I GGNCC 3 cut(s) 217, 1483, 1484
SchI GAGTC 1 cut(s) 709
ScrFI CCNGG 1 cut(s) 1488
SduI GDGCHC 1 cut(s) 1487
SfaNI GCATC 3 cut(s) 259, 879, 1197
SfuI TTCGAA 2 cut(s) 1027, 1068
SinI GGWCC 1 cut(s) 217
Sse9I AATT 5 cut(s) 245, 661, 928, 1109, 1558
SsiI CCGC 8 cut(s) 215, 300, 335, 747, 985, 993, 1047, 1212
SspMI CTAG 4 cut(s) 563, 1310, 1343, 1500
StyD4I CCNGG 1 cut(s) 1486
StyI CCWWGG 1 cut(s) 1352
TaaI ACNGT 9 cut(s) 115, 691, 697, 730, 1141, 1184, 1286, 1375, 1567
TaiI ACGT 4 cut(s) 348, 423, 675, 1131
TaqI TCGA 8 cut(s) 284, 407, 615, 1027, 1068, 1243, 1367, 1493
TasI AATT 5 cut(s) 245, 661, 928, 1109, 1558
TatI WGTACW 1 cut(s) 1377
TauI GCSGC 2 cut(s) 749, 1215
TfiI GAWTC 3 cut(s) 5, 286, 1255
Tru1I TTAA 1 cut(s) 273
Tru9I TTAA 1 cut(s) 273
TscAI CASTG 8 cut(s) 442, 769, 975, 1127, 1146, 1237, 1572, 1630
TseFI GTSAC 2 cut(s) 668, 691
TseI GCWGC 6 cut(s) 652, 749, 1062, 1163, 1166, 1597
Tsp45I GTSAC 2 cut(s) 668, 691
TspDTI ATGAA 6 cut(s) 38, 144, 483, 1138, 1381, 1397
TspGWI ACGGA 3 cut(s) 611, 723, 1149
TspRI CASTG 8 cut(s) 442, 769, 975, 1127, 1146, 1237, 1572, 1630
VpaK11BI GGWCC 1 cut(s) 217
XapI RAATTY 2 cut(s) 661, 1558
XceI RCATGY 1 cut(s) 465
XmiI GTMKAC 1 cut(s) 1131
XmnI GAANNNNTTC 2 cut(s) 151, 1388
XspI CTAG 4 cut(s) 563, 1310, 1343, 1500
ZraI GACGTC 2 cut(s) 421, 1129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.