Rorug07G0301500

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000522
Physical Location & Seq
Reverse (-)
29906153 .. 29909626
3474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0301500.1

Sequence Viewer

Length: 2757 bp
ATGGCTACTGAAGCAATGAAAGTATTGAGAAAGCAAGCCAAGAACACTGAGAAGAGATGTTCAAGCTCAAGCTCCATAGTAGGAGGACACTTCAACGATCCACATCAAGCTCATTTACTGTGCGCAAACCAATTCTGCCAGTGTGAATCTCTGTCATTGAGTAGCAAGCTCACATATAATCCTGCTCCATCGAGTTCAATAGCTCCAACCCAGCAGCATTCGGAGCATCTGCAGCAGTCATTTCTTGCTTCATCGTCCACAAATCATCAGACTTCAAGAATGAAAGTGAAATCCCAACACAAAGAGGGGATTGTATCTTGGCAGTCATCCAAAATGCGCAGAGCCACTAGACGAATAAAGGTAATCAGTTTATTTAAAAGGTTGAATGATGGAACAAAGCGAAGCAACTTTCTGAGGGGCAATTGTCAAGACAAATCAAGGGAAGAAAATGATCATACCAGCCAGGACAAAGTATTTCACTTCGGCATAAATTCTGTTGAATTGAATAGCAGAGCCCAATGGAGCCTCTATACAGATAACTGCTACTTGAACCAAAATCTCAGCAACAAGTACCGACCACGGCTTCTCAAGGACATTGCAGGCCATGAAATGGTTATTAAGGCCATAACCAATGCAATTCAGCAGAATAAGATTGCACCGCTTTATCTTTTCTATGGTGCAAGAGGCTCCGGGAAGACATCTATTGCTAGAATATTAGCATTGGCCTTGAACTGTGAATCCAGCTCATTTATCAAACCGTGCTGGAAATGCCAAGGATGCTCCAGAAGCCTTTCTATTATGGAGTTGTGTTCTGGAAGTACAGTTGCTGGATTCAAAAGGATTAGGAAACTCCTTCAAAGTACATCATCTTTGCAAGCAACACCAGGATTTAAGGTATTTATCCTTGAAGAGTGCGAATCGTTTAGTACAGAAGCATGGGACGAACTTTCAGGCATAGCAAATGGAGCTTACAATGCTGCTGTAGTGTTCATCCTTATCACTACAAATGCAGACATGGTGCCTAGAACCATATCATCAAGATGTCAGAAATTCTTCTTTCCCAAACTCAGAGACAGTGATATCATGCTCAAGTTGACTAGAATTGCAGCCCAAGAGGGTATAAGGATTGAAAAAGAAGCAATCAGACTGGTTACTACCAAAGCACAAGGATCTTTGCGAGAAGCTGAAAACATATTAGACCAGCTGATATTACTGGGGCCAAAGATAACAAGTTCCATGGCACAACAGCTTGTAGGTCTTGTTCCACATGATAAGCTCACTGAATTGCTCACAATGGCACTTTCCGGAGACACTATGCAAACCCTGAGATGTGCAAGGCAGCTGATTGGAACTGGTATTGAACCACAAATGTTTGTATCACAATTGGTTTCGCACATCACCAACATGCTTTTCAGTGAGGCTGATGCTGTTCCTGAAACCTCTTCTTCTACTCACTTATCTACAGATAAGGAACTTCTGCAAGGAGGATCAGAGCACAAAAATAATCAATCAGTGGCACTGTATTGTGCCTTACAAATATTAGCTGCAGTAGAGAAGCAACCCAGGTCTTCAACTGACCAAACCTTGCAGATATTTAATGCTCTTTTACAAATTGCTTCTAGAGATGCTTCCAGCAAAAGATCTTCTGGACCTGTCCTATCCCCAGTAGCAGATTACAAGTCCAATGCAGAAATGGAGTTGCATAGCCTAACCCACAGCAGGTACTTGGTGAACCACATTGCCATCAGCAACACTGCTGAGCAACAAGAGATGGACAATACGAAACAAAAGTCTACCATCAAAAGCATTACGAAACAGACAAATTGCAGAGACAAGGCAGTTGATGGAGAGACCCACTTTACTTGCACCAGTGAAATGGAAGAAATTTGGCTAAACATGTTGGGCAGGATGCATAGTTCATATTTGAAAGACTTTTTGCGCCACAAAGTGAAGCTTGCATCATTAACTATAACTAGTGCCAATGCAATTTTGCATTTGATGTTCAAAAGACCAGAAGACAAGCAGATCGCTCAGATGTCTGAAGAACACCTATCGGAGACTCTAGAGAGCGCTATAGGATGCCCAATTATTCTAAATATGAGCCTAGAGCCTGTGGACTTGAAAATCTCAGACGAAAACTCGGCCTCCAGTTATCAGACACAGCTAGTAGAGAGCAGTCATTTTAACCATGGAACAAAATTTGTTCCTGAACCTGTTTATGAAGTAGATCTTGGAGCTGCAATGCAACACAGTGGTCAGATGAAAACCTTCTCCTTAAACAGAGAGCAAGTTAAGGCCAACAAAGCTGATGGCTGCAAACTGATATCTAAAAGGCAAGACAAATACAGTACAAGCATAAAGGAGGAAACCATTGCTCAGCCACAGGAGACAACACCCTTCAATGATCTTTTAAGACAAAAGAACCAAGAGGATGCATCTATTGATTCGATAACAGATTGTTTGAGTTCTCTGATGGACACAACTCGTACAATGAGGACACAAAGGCCCCAGAAGAAGTGGTTGTCTGTATCCTCTGCTCAACGAAGTGACGCAAGCATTGAACCATACAGTCAAGATATATCGTTTGACAATGCAAAGACAAAGATAAAGAGAAGAGCAAAATCCCAAAAGCTTCCAAAAGCATTCTTTAAGGTTAAAGAACTTCATGATTCTCAAGATGGTCCAAGAAACAGAGGCCTGCACTGCAGCTGGAGTTGCAAGGATATATGCTGTCAGCAAAAACAAAAAATCACATAG

Protein Analysis

918

Amino Acids

102.74

Weight (kDa)

9.08

Isoelectric Point (pI)

48.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol3_delta2 PF13177 202 - 356 1.9e-19 DNA polymerase III, delta subunit
AAA PF00004 222 - 353 2e-08 ATPase family associated with various cellular activities (AAA)
DNAX_ATPase_lid PF22608 361 - 406 9.6e-08 DNA polymerase III clamp loader subunit, ATPase lid domain
DNA_pol3_gamma3 PF12169 411 - 494 8.1e-06 DNA polymerase III subunits gamma and tau domain III
DnaA_N-like_STI PF23007 621 - 702 2.4e-18 STICHEL, DnaA_N-like alpha-beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000522)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02810 AT2G47550 AT4G02330
fragaria_vesca FvH4_2g25960 FvH4_2g25960 FvH4_2g25970 FvH4_5g35570 FvH4_5g35580 FvH4_5g35590
malus_domestica MD03G1290900.v1.1 MD03G1291000.v1.1 MD08G1195600.v1.1 MD11G1307400.v1.1 MD11G1307500.v1.1
prunus_persica Prupe.1G529400_v2.0.a1 Prupe.1G529500_v2.0.a1 Prupe.8G263900_v2.0.a1 Prupe.8G264000_v2.0.a1
pyrus_communis pycom08g16800
rosa_chinensis RchiOBHm_Chr6g0294321 RchiOBHm_Chr6g0294331 RchiOBHm_Chr7g0236951 RchiOBHm_Chr7g0236971 RchiOBHm_Chr7g0237041 RchiOBHm_Chr7g0237051
rosa_laevigata RLG00000001034 RLG00000001035 RLG00000001039 RLG00000001043 RLG00000001045 RLG00000011880 RLG00000011881 RLG00000034181
rosa_multiflora Rmu_co8086188.1_g000001 Rmu_co8458485.1_g000001 Rmu_sc0000272.1_g000007 Rmu_sc0000272.1_g000009 Rmu_sc0002131.1_g000030 Rmu_sc0002131.1_g000037 Rmu_sc0005369.1_g000005 Rmu_sc0005369.1_g000008 Rmu_sc0007991.1_g000005 Rmu_sc0007991.1_g000013 Rmu_sc0033563.1_g000001
rosa_roxburghii Rroxscaffold_3G00224540 Rroxscaffold_3G00224600 Rroxscaffold_3G00224650 Rroxscaffold_3G00224660 Rroxscaffold_7G00173470 Rroxscaffold_7G00173480
rosa_rugosa Rorug06G0243400 Rorug06G0243500 Rorug07G0301200 Rorug07G0301500 Rorug07G0301600
rosa_samantha Rh6AG356000 Rh6AG356100 Rh6BG362600 Rh6BG362800 Rh6CG370000 Rh6CG370100 Rh6DG356500 Rh6DG356600 Rh7AG456200 Rh7AG456500 Rh7AG457500 Rh7AG457600 Rh7BG427500 Rh7BG427700 Rh7BG428400 Rh7BG428500 Rh7CG475100 Rh7CG475300 Rh7CG475700 Rh7CG475800 Rh7DG444100 Rh7DG444300 Rh7DG444700 Rh7DG444900
rosa_wichuraiana Rw6G031020 Rw6G031030 Rw7G037870 Rw7G037880 Rw7G037920 Rw7G037940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 124, 338
Acc36I ACCTGC 1 cut(s) 1710
AccB1I GGYRCC 1 cut(s) 1018
AccB7I CCANNNNNTGG 1 cut(s) 610
AccI GTMKAC 1 cut(s) 1793
AccIII TCCGGA 1 cut(s) 1304
AciI CCGC 1 cut(s) 659
AclWI GGATC 3 cut(s) 92, 1177, 1495
AcsI RAATTY 4 cut(s) 490, 1049, 1884, 2198
AcuI CTGAAG 2 cut(s) 30, 2061
AdeI CACNNNGTG 1 cut(s) 1948
AfaI GTAC 7 cut(s) 572, 820, 862, 928, 1724, 2350, 2488
AfeI AGCGCT 1 cut(s) 2071
AfiI CCNNNNNNNGG 2 cut(s) 610, 1719
AflIII ACRYGT 1 cut(s) 1896
AhlI ACTAGT 1 cut(s) 1973
AjnI CCWGG 3 cut(s) 462, 883, 1562
AjuI GAANNNNNNNTTGG 4 cut(s) 1394, 1426, 2214, 2246
AloI GAACNNNNNNTCC 4 cut(s) 722, 754, 1900, 1932
Alw21I GWGCWC 1 cut(s) 1497
Alw26I GTCTC 6 cut(s) 1065, 1302, 1824, 1844, 2051, 2381
AlwI GGATC 3 cut(s) 92, 1177, 1495
AlwNI CAGNNNCTG 1 cut(s) 827
Aor13HI TCCGGA 1 cut(s) 1304
Aor51HI AGCGCT 1 cut(s) 2071
AoxI GGCC 8 cut(s) 601, 621, 723, 1217, 2142, 2295, 2504, 2695
ApeKI GCWGC 9 cut(s) 214, 232, 977, 1106, 1339, 1544, 2237, 2313, 2706
ApoI RAATTY 4 cut(s) 490, 1049, 1884, 2198
ArsI GACNNNNNNTTYG 2 cut(s) 1775, 1807
Asp700I GAANNNNTTC 3 cut(s) 790, 1052, 2267
AspLEI GCGC 4 cut(s) 125, 339, 1941, 2072
AspS9I GGNCC 4 cut(s) 1217, 1649, 2505, 2681
AsuC2I CCSGG 1 cut(s) 691
AsuHPI GGTGA 2 cut(s) 1390, 1741
AvaII GGWCC 2 cut(s) 1649, 2681
BanI GGYRCC 1 cut(s) 1018
BanII GRGCYC 1 cut(s) 517
BbsI GAAGAC 3 cut(s) 701, 1560, 2022
Bbv12I GWGCWC 1 cut(s) 1497
BbvI GCAGC 9 cut(s) 226, 244, 964, 1118, 1351, 1531, 2224, 2300, 2718
BccI CCATC 9 cut(s) 196, 383, 1751, 1765, 1805, 1838, 2303, 2467, 2672
BceAI ACGGC 1 cut(s) 596
BciT130I CCWGG 3 cut(s) 464, 885, 1564
BciVI GTATCC 1 cut(s) 2539
BclI TGATCA 1 cut(s) 451
BcnI CCSGG 1 cut(s) 691
BcoDI GTCTC 6 cut(s) 1065, 1302, 1824, 1844, 2051, 2381
BcuI ACTAGT 1 cut(s) 1973
BfaI CTAG 9 cut(s) 348, 708, 1023, 1098, 1620, 1974, 2063, 2105, 2165
BfmI CTRYAG 6 cut(s) 230, 981, 1461, 1545, 2073, 2704
BfoI RGCGCY 1 cut(s) 2073
BfuAI ACCTGC 1 cut(s) 1710
BfuI GTATCC 1 cut(s) 2539
BglII AGATCT 2 cut(s) 1640, 2227
BisI GCNGC 9 cut(s) 215, 233, 978, 1107, 1340, 1545, 2238, 2314, 2707
BlpI GCTNAGC 2 cut(s) 1758, 2376
BlsI GCNGC 9 cut(s) 216, 234, 979, 1108, 1341, 1546, 2239, 2315, 2708
Bme1390I CCNGG 4 cut(s) 464, 691, 885, 1564
Bme18I GGWCC 2 cut(s) 1649, 2681
BmgT120I GGNCC 4 cut(s) 1217, 1649, 2505, 2681
BmiI GGNNCC 5 cut(s) 524, 688, 1020, 1218, 2507
BmrFI CCNGG 4 cut(s) 464, 691, 885, 1564
BmrI ACTGGG 2 cut(s) 1223, 1658
BmsI GCATC 9 cut(s) 235, 767, 1414, 1615, 1899, 1967, 2069, 2422, 2444
BmuI ACTGGG 2 cut(s) 1223, 1658
BpiI GAAGAC 3 cut(s) 701, 1560, 2022
BpmI CTGGAG 3 cut(s) 766, 2131, 2731
Bpu1102I GCTNAGC 2 cut(s) 1758, 2376
BpuEI CTTGAG 4 cut(s) 52, 572, 1073, 2658
BpuMI CCSGG 1 cut(s) 691
BsaBI GATNNNNATC 1 cut(s) 102
BsaI GGTCTC 1 cut(s) 1844
BsaJI CCNNGG 5 cut(s) 578, 772, 1236, 1562, 2188
BsaWI WCCGGW 1 cut(s) 1304
BsaXI ACNNNNNCTCC 2 cut(s) 2129, 2159
Bsc4I CCNNNNNNNGG 2 cut(s) 610, 1719
Bse1I ACTGG 7 cut(s) 139, 1152, 1218, 1357, 1664, 1869, 2148
Bse3DI GCAATG 5 cut(s) 21, 594, 1737, 2247, 2370
Bse8I GATNNNNATC 1 cut(s) 102
BseAI TCCGGA 1 cut(s) 1304
BseBI CCWGG 3 cut(s) 464, 885, 1564
BseDI CCNNGG 5 cut(s) 578, 772, 1236, 1562, 2188
BseGI GGATG 6 cut(s) 326, 782, 990, 1914, 2084, 2437
BseJI GATNNNNATC 1 cut(s) 102
BseLI CCNNNNNNNGG 2 cut(s) 610, 1719
BseMI GCAATG 5 cut(s) 21, 594, 1737, 2247, 2370
BseMII CTCAG 9 cut(s) 39, 404, 574, 1081, 1316, 1749, 2045, 2142, 2390
BseNI ACTGG 7 cut(s) 139, 1152, 1218, 1357, 1664, 1869, 2148
BseXI GCAGC 9 cut(s) 226, 244, 964, 1118, 1351, 1531, 2224, 2300, 2718
BseYI CCCAGC 1 cut(s) 210
BsgI GTGCAG 1 cut(s) 2684
BshFI GGCC 8 cut(s) 603, 623, 725, 1219, 2144, 2297, 2506, 2697
BshNI GGYRCC 1 cut(s) 1018
BsiHKAI GWGCWC 1 cut(s) 1497
BsiSI CCGG 2 cut(s) 690, 1305
BslFI GGGAC 1 cut(s) 953
BslI CCNNNNNNNGG 2 cut(s) 610, 1719
BsmAI GTCTC 6 cut(s) 1065, 1302, 1824, 1844, 2051, 2381
BsmFI GGGAC 1 cut(s) 953
BsmI GAATGC 2 cut(s) 217, 2642
BsnI GGCC 8 cut(s) 603, 623, 725, 1219, 2144, 2297, 2506, 2697
Bso31I GGTCTC 1 cut(s) 1844
Bsp1286I GDGCHC 2 cut(s) 517, 1497
Bsp13I TCCGGA 1 cut(s) 1304
Bsp143I GATC 8 cut(s) 97, 451, 1169, 1487, 1640, 2025, 2227, 2404
Bsp1720I GCTNAGC 2 cut(s) 1758, 2376
Bsp19I CCATGG 2 cut(s) 1236, 2188
BspACI CCGC 1 cut(s) 659
BspANI GGCC 8 cut(s) 603, 623, 725, 1219, 2144, 2297, 2506, 2697
BspCNI CTCAG 9 cut(s) 40, 405, 573, 1080, 1317, 1750, 2044, 2141, 2389
BspEI TCCGGA 1 cut(s) 1304
BspHI TCATGA 1 cut(s) 2665
BspLI GGNNCC 5 cut(s) 524, 688, 1020, 1218, 2507
BspMAI CTGCAG 3 cut(s) 234, 1549, 2708
BspMI ACCTGC 1 cut(s) 1710
BspPI GGATC 3 cut(s) 92, 1177, 1495
BspQI GCTCTTC 1 cut(s) 2608
BspT107I GGYRCC 1 cut(s) 1018
BspTNI GGTCTC 1 cut(s) 1844
BsrDI GCAATG 5 cut(s) 21, 594, 1737, 2247, 2370
BsrI ACTGG 7 cut(s) 139, 1152, 1218, 1357, 1664, 1869, 2148
BssECI CCNNGG 5 cut(s) 578, 772, 1236, 1562, 2188
BssMI GATC 8 cut(s) 97, 451, 1169, 1487, 1640, 2025, 2227, 2404
BssT1I CCWWGG 3 cut(s) 772, 1236, 2188
Bst2UI CCWGG 3 cut(s) 464, 885, 1564
Bst4CI ACNGT 9 cut(s) 120, 734, 759, 823, 1076, 1521, 2252, 2348, 2570
Bst6I CTCTTC 4 cut(s) 47, 903, 1447, 2608
BstC8I GCNNGC 7 cut(s) 36, 167, 601, 876, 1956, 2554, 2699
BstDEI CTNAG 9 cut(s) 48, 413, 560, 1067, 1325, 1758, 2031, 2128, 2376
BstDSI CCRYGG 3 cut(s) 578, 1236, 2188
BstF5I GGATG 6 cut(s) 326, 782, 990, 1914, 2084, 2437
BstH2I RGCGCY 1 cut(s) 2073
BstHHI GCGC 4 cut(s) 125, 339, 1941, 2072
BstKTI GATC 8 cut(s) 100, 454, 1172, 1490, 1643, 2028, 2230, 2407
BstMAI GTCTC 6 cut(s) 1065, 1302, 1824, 1844, 2051, 2381
BstMBI GATC 8 cut(s) 97, 451, 1169, 1487, 1640, 2025, 2227, 2404
BstNI CCWGG 3 cut(s) 464, 885, 1564
BstNSI RCATGY 2 cut(s) 1408, 1900
BstSCI CCNGG 4 cut(s) 462, 689, 883, 1562
BstSFI CTRYAG 6 cut(s) 230, 981, 1461, 1545, 2073, 2704
BstV1I GCAGC 9 cut(s) 226, 244, 964, 1118, 1351, 1531, 2224, 2300, 2718
BstV2I GAAGAC 3 cut(s) 701, 1560, 2022
BstX2I RGATCY 3 cut(s) 1169, 1640, 2227
BstXI CCANNNNNNTGG 1 cut(s) 1876
BstYI RGATCY 3 cut(s) 1169, 1640, 2227
BsuI GTATCC 1 cut(s) 2539
BsuRI GGCC 8 cut(s) 603, 623, 725, 1219, 2144, 2297, 2506, 2697
BtgI CCRYGG 3 cut(s) 578, 1236, 2188
BtsCI GGATG 6 cut(s) 326, 782, 990, 1914, 2084, 2437
BtsI GCAGTG 2 cut(s) 1752, 2701
BveI ACCTGC 1 cut(s) 1710
Cac8I GCNNGC 7 cut(s) 36, 167, 601, 876, 1956, 2554, 2699
CaiI CAGNNNCTG 1 cut(s) 827
CciI TCATGA 1 cut(s) 2665
CfoI GCGC 4 cut(s) 125, 339, 1941, 2072
Cfr13I GGNCC 4 cut(s) 1217, 1649, 2505, 2681
CseI GACGC 1 cut(s) 2558
Csp6I GTAC 7 cut(s) 571, 819, 861, 927, 1723, 2349, 2487
CspCI CAANNNNNGTGG 2 cut(s) 1844, 1879
CviQI GTAC 7 cut(s) 571, 819, 861, 927, 1723, 2349, 2487
DdeI CTNAG 9 cut(s) 48, 413, 560, 1067, 1325, 1758, 2031, 2128, 2376
DpnI GATC 8 cut(s) 99, 453, 1171, 1489, 1642, 2027, 2229, 2406
DpnII GATC 8 cut(s) 97, 451, 1169, 1487, 1640, 2025, 2227, 2404
DraI TTTAAA 1 cut(s) 376
DraIII CACNNNGTG 1 cut(s) 1948
Eam1104I CTCTTC 4 cut(s) 47, 903, 1447, 2608
EarI CTCTTC 4 cut(s) 47, 903, 1447, 2608
Eco130I CCWWGG 3 cut(s) 772, 1236, 2188
Eco147I AGGCCT 1 cut(s) 2697
Eco24I GRGCYC 1 cut(s) 517
Eco31I GGTCTC 1 cut(s) 1844
Eco32I GATATC 2 cut(s) 1081, 2325
Eco47I GGWCC 2 cut(s) 1649, 2681
Eco47III AGCGCT 1 cut(s) 2071
Eco57I CTGAAG 2 cut(s) 30, 2061
EcoO109I RGGNCCY 1 cut(s) 2505
EcoRII CCWGG 3 cut(s) 462, 883, 1562
EcoRV GATATC 2 cut(s) 1081, 2325
EcoT14I CCWWGG 3 cut(s) 772, 1236, 2188
EcoT22I ATGCAT 2 cut(s) 1914, 2437
EcoT38I GRGCYC 1 cut(s) 517
ErhI CCWWGG 3 cut(s) 772, 1236, 2188
FalI AAGNNNNNCTT 4 cut(s) 1938, 1970, 2214, 2246
FaqI GGGAC 1 cut(s) 953
FbaI TGATCA 1 cut(s) 451
FblI GTMKAC 1 cut(s) 1793
Fnu4HI GCNGC 9 cut(s) 215, 233, 978, 1107, 1340, 1545, 2238, 2314, 2707
FokI GGATG 6 cut(s) 313, 789, 977, 1921, 2091, 2444
FriOI GRGCYC 1 cut(s) 517
Fsp4HI GCNGC 9 cut(s) 215, 233, 978, 1107, 1340, 1545, 2238, 2314, 2707
FspBI CTAG 9 cut(s) 348, 708, 1023, 1098, 1620, 1974, 2063, 2105, 2165
FspI TGCGCA 2 cut(s) 124, 338
GlaI GCGC 4 cut(s) 124, 338, 1940, 2071
GluI GCNGC 9 cut(s) 215, 233, 978, 1107, 1340, 1545, 2238, 2314, 2707
GsaI CCCAGC 1 cut(s) 214
GsuI CTGGAG 3 cut(s) 766, 2131, 2731
HaeII RGCGCY 1 cut(s) 2073
HaeIII GGCC 8 cut(s) 603, 623, 725, 1219, 2144, 2297, 2506, 2697
HapII CCGG 2 cut(s) 690, 1305
HgaI GACGC 1 cut(s) 2558
HhaI GCGC 4 cut(s) 125, 339, 1941, 2072
Hin6I GCGC 4 cut(s) 123, 337, 1939, 2070
HinP1I GCGC 4 cut(s) 123, 337, 1939, 2070
HincII GTYRAC 1 cut(s) 1095
HindII GTYRAC 1 cut(s) 1095
HindIII AAGCTT 2 cut(s) 1952, 2630
HinfI GANTC 7 cut(s) 146, 737, 831, 917, 2059, 2444, 2669
HpaII CCGG 2 cut(s) 690, 1305
HphI GGTGA 2 cut(s) 1390, 1741
Hpy166II GTNNAC 5 cut(s) 258, 1095, 1732, 1794, 2116
Hpy8I GTNNAC 5 cut(s) 258, 1095, 1732, 1794, 2116
HpyAV CCTTC 3 cut(s) 863, 2278, 2407
HpyCH4III ACNGT 9 cut(s) 120, 734, 759, 823, 1076, 1521, 2252, 2348, 2570
HpyF3I CTNAG 9 cut(s) 48, 413, 560, 1067, 1325, 1758, 2031, 2128, 2376
HspAI GCGC 4 cut(s) 123, 337, 1939, 2070
Kpn2I TCCGGA 1 cut(s) 1304
Ksp22I TGATCA 1 cut(s) 451
Kzo9I GATC 8 cut(s) 97, 451, 1169, 1487, 1640, 2025, 2227, 2404
LguI GCTCTTC 1 cut(s) 2608
LmnI GCTCC 9 cut(s) 77, 190, 208, 223, 522, 692, 785, 965, 2234
Lsp1109I GCAGC 9 cut(s) 226, 244, 964, 1118, 1351, 1531, 2224, 2300, 2718
LweI GCATC 9 cut(s) 235, 767, 1414, 1615, 1899, 1967, 2069, 2422, 2444
MaeI CTAG 9 cut(s) 348, 708, 1023, 1098, 1620, 1974, 2063, 2105, 2165
MaeIII GTNAC 2 cut(s) 1150, 2546
MalI GATC 8 cut(s) 99, 453, 1171, 1489, 1642, 2027, 2229, 2406
MboI GATC 8 cut(s) 97, 451, 1169, 1487, 1640, 2025, 2227, 2404
MfeI CAATTG 2 cut(s) 421, 1382
MflI RGATCY 3 cut(s) 1169, 1640, 2227
MhlI GDGCHC 2 cut(s) 517, 1497
MlyI GAGTC 1 cut(s) 2053
MmeI TCCRAC 1 cut(s) 230
Mph1103I ATGCAT 2 cut(s) 1914, 2437
MroI TCCGGA 1 cut(s) 1304
MroXI GAANNNNTTC 3 cut(s) 790, 1052, 2267
MslI CAYNNNNRTG 2 cut(s) 1039, 1403
MspA1I CMGCKG 3 cut(s) 1204, 1342, 2709
MspI CCGG 2 cut(s) 690, 1305
MspR9I CCNGG 4 cut(s) 464, 691, 885, 1564
MunI CAATTG 2 cut(s) 421, 1382
Mva1269I GAATGC 2 cut(s) 217, 2642
MvaI CCWGG 3 cut(s) 464, 885, 1564
NciI CCSGG 1 cut(s) 691
NcoI CCATGG 2 cut(s) 1236, 2188
NdeII GATC 8 cut(s) 97, 451, 1169, 1487, 1640, 2025, 2227, 2404
NlaIV GGNNCC 5 cut(s) 524, 688, 1020, 1218, 2507
NmeAIII GCCGAG 1 cut(s) 2120
NmuCI GTSAC 1 cut(s) 2546
NsbI TGCGCA 2 cut(s) 124, 338
NsiI ATGCAT 2 cut(s) 1914, 2437
NspI RCATGY 2 cut(s) 1408, 1900
PagI TCATGA 1 cut(s) 2665
PceI AGGCCT 1 cut(s) 2697
PciI ACATGT 1 cut(s) 1896
PciSI GCTCTTC 1 cut(s) 2608
PctI GAATGC 2 cut(s) 217, 2642
PdmI GAANNNNTTC 3 cut(s) 790, 1052, 2267
PfeI GAWTC 6 cut(s) 146, 737, 831, 917, 2444, 2669
PflMI CCANNNNNTGG 1 cut(s) 610
PfoI TCCNGGA 1 cut(s) 689
PkrI GCNGC 9 cut(s) 216, 234, 979, 1108, 1341, 1546, 2239, 2315, 2708
PleI GAGTC 1 cut(s) 2053
PpsI GAGTC 1 cut(s) 2053
PscI ACATGT 1 cut(s) 1896
Psp6I CCWGG 3 cut(s) 462, 883, 1562
PspFI CCCAGC 1 cut(s) 210
PspGI CCWGG 3 cut(s) 462, 883, 1562
PspN4I GGNNCC 5 cut(s) 524, 688, 1020, 1218, 2507
PspPI GGNCC 4 cut(s) 1217, 1649, 2505, 2681
PsrI GAACNNNNNNTAC 2 cut(s) 1245, 1277
PstI CTGCAG 3 cut(s) 234, 1549, 2708
PstNI CAGNNNCTG 1 cut(s) 827
PsuI RGATCY 3 cut(s) 1169, 1640, 2227
PvuII CAGCTG 3 cut(s) 1204, 1342, 2709
RsaI GTAC 7 cut(s) 572, 820, 862, 928, 1724, 2350, 2488
RsaNI GTAC 7 cut(s) 571, 819, 861, 927, 1723, 2349, 2487
RseI CAYNNNNRTG 2 cut(s) 1039, 1403
SapI GCTCTTC 1 cut(s) 2608
SatI GCNGC 9 cut(s) 215, 233, 978, 1107, 1340, 1545, 2238, 2314, 2707
Sau3AI GATC 8 cut(s) 97, 451, 1169, 1487, 1640, 2025, 2227, 2404
Sau96I GGNCC 4 cut(s) 1217, 1649, 2505, 2681
SchI GAGTC 1 cut(s) 2053
ScrFI CCNGG 4 cut(s) 464, 691, 885, 1564
SduI GDGCHC 2 cut(s) 517, 1497
SfaNI GCATC 9 cut(s) 235, 767, 1414, 1615, 1899, 1967, 2069, 2422, 2444
SfcI CTRYAG 6 cut(s) 230, 981, 1461, 1545, 2073, 2704
SinI GGWCC 2 cut(s) 1649, 2681
SmiMI CAYNNNNRTG 2 cut(s) 1039, 1403
SmlI CTYRAG 4 cut(s) 67, 587, 1088, 2673
SmoI CTYRAG 4 cut(s) 67, 587, 1088, 2673
SpeI ACTAGT 1 cut(s) 1973
SseBI AGGCCT 1 cut(s) 2697
SsiI CCGC 1 cut(s) 659
SspI AATATT 2 cut(s) 714, 1539
SspMI CTAG 9 cut(s) 348, 708, 1023, 1098, 1620, 1974, 2063, 2105, 2165
StuI AGGCCT 1 cut(s) 2697
StyD4I CCNGG 4 cut(s) 462, 689, 883, 1562
StyI CCWWGG 3 cut(s) 772, 1236, 2188
TaaI ACNGT 9 cut(s) 120, 734, 759, 823, 1076, 1521, 2252, 2348, 2570
TaqI TCGA 2 cut(s) 191, 2447
TatI WGTACW 4 cut(s) 818, 860, 926, 2348
TfiI GAWTC 6 cut(s) 146, 737, 831, 917, 2444, 2669
TseFI GTSAC 1 cut(s) 2546
TseI GCWGC 9 cut(s) 214, 232, 977, 1106, 1339, 1544, 2237, 2313, 2706
Tsp45I GTSAC 1 cut(s) 2546
TspDTI ATGAA 9 cut(s) 32, 240, 296, 621, 979, 1908, 2235, 2276, 2654
Van91I CCANNNNNTGG 1 cut(s) 610
VpaK11BI GGWCC 2 cut(s) 1649, 2681
XapI RAATTY 4 cut(s) 490, 1049, 1884, 2198
XbaI TCTAGA 2 cut(s) 1619, 2062
XceI RCATGY 2 cut(s) 1408, 1900
XcmI CCANNNNNNNNNTGG 1 cut(s) 1690
XmiI GTMKAC 1 cut(s) 1793
XmnI GAANNNNTTC 3 cut(s) 790, 1052, 2267
XspI CTAG 9 cut(s) 348, 708, 1023, 1098, 1620, 1974, 2063, 2105, 2165
Zsp2I ATGCAT 2 cut(s) 1914, 2437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.