Rh7DG444900

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000522
Physical Location & Seq
Forward (+)
63166120 .. 63167331
1212 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG444900.1

Sequence Viewer

Length: 702 bp
ATGAATGCAGCTGTGGTGGCAGAAGGGTTTGTGGGAGTCAACATAACCATTCGTAACACGGCTGGACCAAGTAAAATGCAGGCTGTGGCGGTCAGAAACGGCGCGGACTTGTCAGTCTTCTACAGCTGCAGCTTTGAAGCCTACCAGGACACCCTGTACGCGCATTCTCTCCGACAATTCTACCGAGAATGTGACATCTATGGAACTGTAGATTTCATATTCGGCAATGCTGCAGTTGTTTTACAAAACTGTAACCTCTACCCTCGCCAGCCACTCAAAGGCCAATCCAATCCCATCACGGCTCAGGGCCGAACTGACCCGAATCAGAATACGGGCACTTCCATCCAGAACTGCACCATTACAGCCGCTGCGGACTTGGCTTCGAGTAACTTCACTGTCAAGACATATCTAGGGAGGCCATGGAAGGAGTATTCGAGGACGGTTTACTTGCAGAATTTCATGGATAGTTTGATTGATCCTGTTGGTTGGCTCGAGTGGAATGGAGATTTTGCACTGAGCACATTGTATTATGCTGAGTATGATAACAGAGGACCTGGATCAGACACTAGTAACAGAGTCACATGGCCTGGTTACCATGTGATCAATGCAACTGATGCTGTTAATTTTACAGTGTCTAACTTTCTGGTAGGGGATAGCTGGTTGCCTCAGACAGGTGTGCCTTATACTGGTGGCTTTGTTTGA

Protein Analysis

233

Amino Acids

25.69

Weight (kDa)

4.91

Isoelectric Point (pI)

28.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 4 - 218 2.3e-107 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000522)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02810 AT2G47550 AT4G02330
fragaria_vesca FvH4_2g25960 FvH4_2g25960 FvH4_2g25970 FvH4_5g35570 FvH4_5g35580 FvH4_5g35590
malus_domestica MD03G1290900.v1.1 MD03G1291000.v1.1 MD08G1195600.v1.1 MD11G1307400.v1.1 MD11G1307500.v1.1
prunus_persica Prupe.1G529400_v2.0.a1 Prupe.1G529500_v2.0.a1 Prupe.8G263900_v2.0.a1 Prupe.8G264000_v2.0.a1
pyrus_communis pycom08g16800
rosa_chinensis RchiOBHm_Chr6g0294321 RchiOBHm_Chr6g0294331 RchiOBHm_Chr7g0236951 RchiOBHm_Chr7g0236971 RchiOBHm_Chr7g0237041 RchiOBHm_Chr7g0237051
rosa_laevigata RLG00000001034 RLG00000001035 RLG00000001039 RLG00000001043 RLG00000001045 RLG00000011880 RLG00000011881 RLG00000034181
rosa_multiflora Rmu_co8086188.1_g000001 Rmu_co8458485.1_g000001 Rmu_sc0000272.1_g000007 Rmu_sc0000272.1_g000009 Rmu_sc0002131.1_g000030 Rmu_sc0002131.1_g000037 Rmu_sc0005369.1_g000005 Rmu_sc0005369.1_g000008 Rmu_sc0007991.1_g000005 Rmu_sc0007991.1_g000013 Rmu_sc0033563.1_g000001
rosa_roxburghii Rroxscaffold_3G00224540 Rroxscaffold_3G00224600 Rroxscaffold_3G00224650 Rroxscaffold_3G00224660 Rroxscaffold_7G00173470 Rroxscaffold_7G00173480
rosa_rugosa Rorug06G0243400 Rorug06G0243500 Rorug07G0301200 Rorug07G0301500 Rorug07G0301600
rosa_samantha Rh6AG356000 Rh6AG356100 Rh6BG362600 Rh6BG362800 Rh6CG370000 Rh6CG370100 Rh6DG356500 Rh6DG356600 Rh7AG456200 Rh7AG456500 Rh7AG457500 Rh7AG457600 Rh7BG427500 Rh7BG427700 Rh7BG428400 Rh7BG428500 Rh7CG475100 Rh7CG475300 Rh7CG475700 Rh7CG475800 Rh7DG444100 Rh7DG444300 Rh7DG444700 Rh7DG444900
rosa_wichuraiana Rw6G031020 Rw6G031030 Rw7G037870 Rw7G037880 Rw7G037920 Rw7G037940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 113
AccII CGCG 2 cut(s) 104, 161
AciI CCGC 4 cut(s) 89, 104, 366, 371
AclWI GGATC 2 cut(s) 470, 565
AcsI RAATTY 1 cut(s) 454
AfaI GTAC 1 cut(s) 158
AfiI CCNNNNNNNGG 3 cut(s) 278, 671, 686
AgsI TTSAA 1 cut(s) 137
AhlI ACTAGT 1 cut(s) 566
AjnI CCWGG 3 cut(s) 144, 553, 586
AluBI AGCT 4 cut(s) 11, 126, 132, 657
AluI AGCT 4 cut(s) 11, 126, 132, 657
Alw21I GWGCWC 1 cut(s) 521
AlwI GGATC 2 cut(s) 470, 565
AlwNI CAGNNNCTG 1 cut(s) 368
Ama87I CYCGRG 1 cut(s) 491
AoxI GGCC 4 cut(s) 280, 307, 416, 584
ApeKI GCWGC 5 cut(s) 8, 126, 129, 230, 368
ApoI RAATTY 1 cut(s) 454
AspLEI GCGC 2 cut(s) 104, 163
AspS9I GGNCC 3 cut(s) 65, 307, 551
AvaI CYCGRG 1 cut(s) 491
AvaII GGWCC 2 cut(s) 65, 551
BaeGI GKGCMC 1 cut(s) 338
BbsI GAAGAC 1 cut(s) 109
Bbv12I GWGCWC 1 cut(s) 521
BbvI GCAGC 5 cut(s) 20, 113, 141, 217, 355
BccI CCATC 2 cut(s) 302, 350
BceAI ACGGC 3 cut(s) 75, 115, 315
BciT130I CCWGG 3 cut(s) 146, 555, 588
BclI TGATCA 1 cut(s) 600
BcuI ACTAGT 1 cut(s) 566
BfaI CTAG 2 cut(s) 410, 567
BfmI CTRYAG 4 cut(s) 121, 127, 207, 231
BisI GCNGC 6 cut(s) 9, 127, 130, 231, 366, 369
BlsI GCNGC 6 cut(s) 10, 128, 131, 232, 367, 370
Bme1390I CCNGG 3 cut(s) 146, 555, 588
Bme18I GGWCC 2 cut(s) 65, 551
BmeT110I CYCGRG 1 cut(s) 491
BmgT120I GGNCC 3 cut(s) 65, 307, 551
BmrFI CCNGG 3 cut(s) 146, 555, 588
BmsI GCATC 1 cut(s) 604
BpiI GAAGAC 1 cut(s) 109
Bpu10I CCTNAGC 1 cut(s) 303
BsaJI CCNNGG 1 cut(s) 419
Bsc4I CCNNNNNNNGG 3 cut(s) 278, 671, 686
Bse1I ACTGG 1 cut(s) 691
Bse3DI GCAATG 1 cut(s) 232
BseBI CCWGG 3 cut(s) 146, 555, 588
BseDI CCNNGG 1 cut(s) 419
BseGI GGATG 1 cut(s) 342
BseLI CCNNNNNNNGG 3 cut(s) 278, 671, 686
BseMI GCAATG 1 cut(s) 232
BseMII CTCAG 4 cut(s) 317, 506, 525, 680
BseNI ACTGG 1 cut(s) 691
BseSI GKGCMC 1 cut(s) 338
BseXI GCAGC 5 cut(s) 20, 113, 141, 217, 355
BsgI GTGCAG 1 cut(s) 337
Bsh1236I CGCG 2 cut(s) 104, 161
BshFI GGCC 4 cut(s) 282, 309, 418, 586
BsiHKAI GWGCWC 1 cut(s) 521
BsiHKCI CYCGRG 1 cut(s) 491
BslI CCNNNNNNNGG 3 cut(s) 278, 671, 686
BsmI GAATGC 2 cut(s) 10, 163
BsnI GGCC 4 cut(s) 282, 309, 418, 586
BsoBI CYCGRG 1 cut(s) 491
Bsp1286I GDGCHC 2 cut(s) 338, 521
Bsp143I GATC 3 cut(s) 475, 557, 600
Bsp19I CCATGG 1 cut(s) 419
BspACI CCGC 4 cut(s) 89, 104, 366, 371
BspANI GGCC 4 cut(s) 282, 309, 418, 586
BspCNI CTCAG 4 cut(s) 316, 507, 526, 679
BspFNI CGCG 2 cut(s) 104, 161
BspMAI CTGCAG 2 cut(s) 131, 235
BspPI GGATC 2 cut(s) 470, 565
BsrDI GCAATG 1 cut(s) 232
BsrI ACTGG 1 cut(s) 691
BssECI CCNNGG 1 cut(s) 419
BssMI GATC 3 cut(s) 475, 557, 600
BssT1I CCWWGG 1 cut(s) 419
Bst2UI CCWGG 3 cut(s) 146, 555, 588
Bst4CI ACNGT 5 cut(s) 208, 251, 397, 442, 631
BstAPI GCANNNNNTGC 1 cut(s) 614
BstC8I GCNNGC 2 cut(s) 81, 269
BstDEI CTNAG 4 cut(s) 303, 515, 534, 666
BstDSI CCRYGG 1 cut(s) 419
BstEII GGTNACC 1 cut(s) 590
BstENI CCTNNNNNAGG 1 cut(s) 669
BstF5I GGATG 1 cut(s) 342
BstFNI CGCG 2 cut(s) 104, 161
BstHHI GCGC 2 cut(s) 104, 163
BstKTI GATC 3 cut(s) 478, 560, 603
BstMBI GATC 3 cut(s) 475, 557, 600
BstMWI GCNNNNNNNGC 3 cut(s) 17, 377, 614
BstNI CCWGG 3 cut(s) 146, 555, 588
BstPI GGTNACC 1 cut(s) 590
BstSCI CCNGG 3 cut(s) 144, 553, 586
BstSFI CTRYAG 4 cut(s) 121, 127, 207, 231
BstSLI GKGCMC 1 cut(s) 338
BstUI CGCG 2 cut(s) 104, 161
BstV1I GCAGC 5 cut(s) 20, 113, 141, 217, 355
BstV2I GAAGAC 1 cut(s) 109
BsuRI GGCC 4 cut(s) 282, 309, 418, 586
BtgI CCRYGG 1 cut(s) 419
BtsCI GGATG 1 cut(s) 342
BtsIMutI CAGTG 3 cut(s) 393, 512, 636
Cac8I GCNNGC 2 cut(s) 81, 269
CaiI CAGNNNCTG 1 cut(s) 368
CfoI GCGC 2 cut(s) 104, 163
Cfr13I GGNCC 3 cut(s) 65, 307, 551
Csp6I GTAC 1 cut(s) 157
CviAII CATG 4 cut(s) 420, 460, 582, 596
CviQI GTAC 1 cut(s) 157
DdeI CTNAG 4 cut(s) 303, 515, 534, 666
DpnI GATC 3 cut(s) 477, 559, 602
DpnII GATC 3 cut(s) 475, 557, 600
DrdI GACNNNNNNGTC 1 cut(s) 113
DseDI GACNNNNNNGTC 1 cut(s) 113
Eco130I CCWWGG 1 cut(s) 419
Eco47I GGWCC 2 cut(s) 65, 551
Eco88I CYCGRG 1 cut(s) 491
Eco91I GGTNACC 1 cut(s) 590
EcoNI CCTNNNNNAGG 1 cut(s) 669
EcoO109I RGGNCCY 1 cut(s) 551
EcoO65I GGTNACC 1 cut(s) 590
EcoRII CCWGG 3 cut(s) 144, 553, 586
EcoT14I CCWWGG 1 cut(s) 419
ErhI CCWWGG 1 cut(s) 419
FaeI CATG 4 cut(s) 423, 463, 585, 599
FatI CATG 4 cut(s) 419, 459, 581, 595
FbaI TGATCA 1 cut(s) 600
Fnu4HI GCNGC 6 cut(s) 9, 127, 130, 231, 366, 369
FokI GGATG 1 cut(s) 329
Fsp4HI GCNGC 6 cut(s) 9, 127, 130, 231, 366, 369
FspBI CTAG 2 cut(s) 410, 567
GlaI GCGC 2 cut(s) 103, 162
GluI GCNGC 6 cut(s) 9, 127, 130, 231, 366, 369
HaeIII GGCC 4 cut(s) 282, 309, 418, 586
HhaI GCGC 2 cut(s) 104, 163
Hin1II CATG 4 cut(s) 423, 463, 585, 599
Hin6I GCGC 2 cut(s) 102, 161
HinP1I GCGC 2 cut(s) 102, 161
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HinfI GANTC 3 cut(s) 36, 322, 576
Hpy166II GTNNAC 2 cut(s) 40, 445
Hpy188I TCNGA 5 cut(s) 95, 173, 327, 562, 669
Hpy188III TCNNGA 2 cut(s) 346, 400
Hpy8I GTNNAC 2 cut(s) 40, 445
HpyAV CCTTC 2 cut(s) 17, 418
HpyCH4III ACNGT 5 cut(s) 208, 251, 397, 442, 631
HpyCH4V TGCA 8 cut(s) 8, 79, 129, 233, 354, 451, 512, 608
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 377, 614
HpyF3I CTNAG 4 cut(s) 303, 515, 534, 666
Hsp92II CATG 4 cut(s) 423, 463, 585, 599
HspAI GCGC 2 cut(s) 102, 161
Ksp22I TGATCA 1 cut(s) 600
Kzo9I GATC 3 cut(s) 475, 557, 600
Lsp1109I GCAGC 5 cut(s) 20, 113, 141, 217, 355
LweI GCATC 1 cut(s) 604
MaeI CTAG 2 cut(s) 410, 567
MaeIII GTNAC 7 cut(s) 53, 191, 251, 386, 569, 577, 590
MalI GATC 3 cut(s) 477, 559, 602
MboI GATC 3 cut(s) 475, 557, 600
MboII GAAGA 1 cut(s) 109
MhlI GDGCHC 2 cut(s) 338, 521
MluCI AATT 3 cut(s) 176, 454, 622
MlyI GAGTC 2 cut(s) 45, 585
MmeI TCCRAC 1 cut(s) 196
MnlI CCTC 6 cut(s) 266, 273, 408, 429, 542, 675
MseI TTAA 1 cut(s) 621
MspA1I CMGCKG 3 cut(s) 11, 126, 368
MspR9I CCNGG 3 cut(s) 146, 555, 588
Mva1269I GAATGC 2 cut(s) 10, 163
MvaI CCWGG 3 cut(s) 146, 555, 588
MvnI CGCG 2 cut(s) 104, 161
MwoI GCNNNNNNNGC 3 cut(s) 17, 377, 614
NcoI CCATGG 1 cut(s) 419
NdeII GATC 3 cut(s) 475, 557, 600
NlaIII CATG 4 cut(s) 423, 463, 585, 599
NmuCI GTSAC 2 cut(s) 191, 577
PaeR7I CTCGAG 1 cut(s) 491
PctI GAATGC 2 cut(s) 10, 163
PfeI GAWTC 1 cut(s) 322
PkrI GCNGC 6 cut(s) 10, 128, 131, 232, 367, 370
PleI GAGTC 2 cut(s) 44, 584
PpsI GAGTC 2 cut(s) 44, 584
PpuMI RGGWCCY 1 cut(s) 551
Psp5II RGGWCCY 1 cut(s) 551
Psp6I CCWGG 3 cut(s) 144, 553, 586
PspEI GGTNACC 1 cut(s) 590
PspGI CCWGG 3 cut(s) 144, 553, 586
PspPI GGNCC 3 cut(s) 65, 307, 551
PspPPI RGGWCCY 1 cut(s) 551
PspXI VCTCGAGB 1 cut(s) 491
PstI CTGCAG 2 cut(s) 131, 235
PstNI CAGNNNCTG 1 cut(s) 368
PvuII CAGCTG 2 cut(s) 11, 126
RsaI GTAC 1 cut(s) 158
RsaNI GTAC 1 cut(s) 157
SaqAI TTAA 1 cut(s) 621
SatI GCNGC 6 cut(s) 9, 127, 130, 231, 366, 369
Sau3AI GATC 3 cut(s) 475, 557, 600
Sau96I GGNCC 3 cut(s) 65, 307, 551
SchI GAGTC 2 cut(s) 45, 585
ScrFI CCNGG 3 cut(s) 146, 555, 588
SduI GDGCHC 2 cut(s) 338, 521
SetI ASST 7 cut(s) 13, 128, 134, 258, 556, 659, 676
SfaNI GCATC 1 cut(s) 604
SfcI CTRYAG 4 cut(s) 121, 127, 207, 231
Sfr274I CTCGAG 1 cut(s) 491
SinI GGWCC 2 cut(s) 65, 551
SlaI CTCGAG 1 cut(s) 491
SmlI CTYRAG 1 cut(s) 491
SmoI CTYRAG 1 cut(s) 491
SpeI ACTAGT 1 cut(s) 566
Sse9I AATT 3 cut(s) 176, 454, 622
SsiI CCGC 4 cut(s) 89, 104, 366, 371
SspMI CTAG 2 cut(s) 410, 567
StyD4I CCNGG 3 cut(s) 144, 553, 586
StyI CCWWGG 1 cut(s) 419
TaaI ACNGT 5 cut(s) 208, 251, 397, 442, 631
TaqI TCGA 3 cut(s) 383, 434, 492
TasI AATT 3 cut(s) 176, 454, 622
TauI GCSGC 1 cut(s) 368
TfiI GAWTC 1 cut(s) 322
Tru1I TTAA 1 cut(s) 621
Tru9I TTAA 1 cut(s) 621
TscAI CASTG 3 cut(s) 400, 519, 636
TseFI GTSAC 2 cut(s) 191, 577
TseI GCWGC 5 cut(s) 8, 126, 129, 230, 368
Tsp45I GTSAC 2 cut(s) 191, 577
TspDTI ATGAA 3 cut(s) 17, 205, 448
TspRI CASTG 3 cut(s) 400, 519, 636
VpaK11BI GGWCC 2 cut(s) 65, 551
XagI CCTNNNNNAGG 1 cut(s) 669
XapI RAATTY 1 cut(s) 454
XhoI CTCGAG 1 cut(s) 491
XspI CTAG 2 cut(s) 410, 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.