Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7C

Orthogroup ID

OG0000522

Physical Location & Seq

Genomic Coordinates Reverse (-)

63872505 .. 63874328

Gene Structure 1824 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7CG475700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1698 bp

ATGGCTTCTAAGCTCTTCAATCCTCTTGCAACTTCATCATTTTCACTATTCCTCATCATTTTCGCTTTGTTTATATCTCAAACTTCAGCTGCAGATGTTCCTCTCAGCATTTCTGTTCCGCCCGAAACCATATGCAAGTCCACTCCACACCCTTCTGTTTGCATTTCCGTCCTCCCTCACAAAAATGCCACTGTCTATGACTTCGGCCGCTTGTCTGTCCAACACTCATTCGTCCAATCCCAAAAGCTTCTGGATTTTATCAACAAATATGTTCAAGGTGGTTCCAGTTTATCACAGCTGGAAATCCAAGCCCTCGAGGATTGCAAGTCGCTTGCATTGTTAAACATTGATTTCTTATCAAACACTCTTGAAACTGTTAACAAAACAAGCGGTGTTCTTCCCAGCTCCGATGCTGACGATGTCCAAACCCTGCTTAGTGCCATCTTAACCAACCAGCAGACGTGTTCTGATGGCCTTCAATCTGTGAGTTCAGTCCAAAATGATCTCTCGGTATCCGTTTCTAATGGTACAAATTTGTACAGTGTCTCGTTAGCGTTGGTTACAAAGGGTTGGGTCCCAAAGGATCACAAAAATAATGGAACACCAAACCAGTCGAAAAAGCACCTCAGTTTCCGAAAGGGGCGATTGTCACTGAAAATGTCAAGCGAAGCGCGTGCAGTTTACGACTCGGTTATAAATCGAAGAAGGCACCTTCTTCAAGCCGGAGATGAAGAAATTTTGGTGAAGGACATTGTGGTTGTGAGCCAAGATGGAAGTGGAAACTTCACTACCATCAATGATGCTATCAATGCTGCACCCAATAACTCTGTTGCTAGTGATGGCTACTTCTTGGTATATGTCACTGCCGGCGTTTATGAAGAGTACGTATCAATTATATCGAAAAAGAAGTACTTGTTGCTGATCGGAGACGGTATTAATCAGACGATCATCACAGGAAACAACAGCGTTGGAGGCGGTTCAACCACCTTCAACTCTGCCACTTTAGCTGTGGTAGCACCAGGGTTTATAGCAATCAACATAACTGTTCGAAACACTGCTGGACCAGGCATGGGGCAAGCAGTTGCTGTTCGAAATGGGGCTGACTTGTCAGTTTTCTACATCTGTAGCTTTGAGGGATACCAAGACACCTTATACACACATTCTATGAGACAATTCTACAGGGAATGTGACATCTACGGAACGGTTGATTTCATCTTTGGAAATGCTGCAGTTGTTTTCCAAAACTGCAACATATACCCTCGTCGTCCCCTCCAAGGCCAATCAGATACCATCACAGCTCAAGGGCGGACCGATGTGAATCAGAACACAGGGACTTCAATCCAGAATTGCACCATTACAGCCGCGGCTGATTTGGCGTCAAGTAACTTCACTGTCAAGACATATCTTGGGAGGCCATGGAAGGAGTATTCGAGGACGGTTTACTTGCAGAATTTCATGGATAGTTTGATCGATCCTGCTGGTTGGCTCGCGTGGAGTGGAGATTTTGCACTGAGCACATTGTATTATGCTGAGTACAATAATAGAGGACCTGGATCAAACACTAGCAACAGAGTTACATGGCCTGGTTACCATGTGATCGGTGCAACTGATGCTGCCAACTTTACAGTCTCCAACTTCATATTGGGGGATAATTGGCTGCCTCAGACAGGAGTGCCTTACACTGGTGGCTTAATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

565

Amino Acids

61.19

Weight (kDa)

5.92

Isoelectric Point (pI)

33.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	42 - 186	3e-26	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	252 - 550	4.2e-135	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000522)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02810 AT2G47550 AT4G02330
fragaria_vesca	FvH4_2g25960 FvH4_2g25960 FvH4_2g25970 FvH4_5g35570 FvH4_5g35580 FvH4_5g35590
malus_domestica	MD03G1290900.v1.1 MD03G1291000.v1.1 MD08G1195600.v1.1 MD11G1307400.v1.1 MD11G1307500.v1.1
prunus_persica	Prupe.1G529400_v2.0.a1 Prupe.1G529500_v2.0.a1 Prupe.8G263900_v2.0.a1 Prupe.8G264000_v2.0.a1
pyrus_communis	pycom08g16800
rosa_chinensis	RchiOBHm_Chr6g0294321 RchiOBHm_Chr6g0294331 RchiOBHm_Chr7g0236951 RchiOBHm_Chr7g0236971 RchiOBHm_Chr7g0237041 RchiOBHm_Chr7g0237051
rosa_laevigata	RLG00000001034 RLG00000001035 RLG00000001039 RLG00000001043 RLG00000001045 RLG00000011880 RLG00000011881 RLG00000034181
rosa_multiflora	Rmu_co8086188.1_g000001 Rmu_co8458485.1_g000001 Rmu_sc0000272.1_g000007 Rmu_sc0000272.1_g000009 Rmu_sc0002131.1_g000030 Rmu_sc0002131.1_g000037 Rmu_sc0005369.1_g000005 Rmu_sc0005369.1_g000008 Rmu_sc0007991.1_g000005 Rmu_sc0007991.1_g000013 Rmu_sc0033563.1_g000001
rosa_roxburghii	Rroxscaffold_3G00224540 Rroxscaffold_3G00224600 Rroxscaffold_3G00224650 Rroxscaffold_3G00224660 Rroxscaffold_7G00173470 Rroxscaffold_7G00173480
rosa_rugosa	Rorug06G0243400 Rorug06G0243500 Rorug07G0301200 Rorug07G0301500 Rorug07G0301600
rosa_samantha	Rh6AG356000 Rh6AG356100 Rh6BG362600 Rh6BG362800 Rh6CG370000 Rh6CG370100 Rh6DG356500 Rh6DG356600 Rh7AG456200 Rh7AG456500 Rh7AG457500 Rh7AG457600 Rh7BG427500 Rh7BG427700 Rh7BG428400 Rh7BG428500 Rh7CG475100 Rh7CG475300 Rh7CG475700 Rh7CG475800 Rh7DG444100 Rh7DG444300 Rh7DG444700 Rh7DG444900
rosa_wichuraiana	Rw6G031020 Rw6G031030 Rw7G037870 Rw7G037880 Rw7G037920 Rw7G037940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	695
AbsI	CCTCGAGG	1 cut(s)	314
AccB1I	GGYRCC	1 cut(s)	708
AccII	CGCG	3 cut(s)	673, 1364, 1490
AciI	CCGC	7 cut(s)	119, 208, 390, 975, 1306, 1362, 1364
AclWI	GGATC	3 cut(s)	591, 1466, 1561
AcoI	YGGCCR	1 cut(s)	205
AcsI	RAATTY	3 cut(s)	532, 735, 1450
AcuI	CTGAAG	1 cut(s)	69
AcyI	GRCGYC	1 cut(s)	1376
AfaI	GTAC	5 cut(s)	529, 539, 884, 911, 1535
AfiI	CCNNNNNNNGG	4 cut(s)	1070, 1274, 1667, 1682
AflIII	ACRYGT	1 cut(s)	461
AgsI	TTSAA	8 cut(s)	19, 275, 371, 479, 719, 981, 991, 1338
AjiI	CACGTC	1 cut(s)	462
AjnI	CCWGG	4 cut(s)	1018, 1063, 1549, 1582
AluBI	AGCT	8 cut(s)	13, 89, 247, 298, 405, 1007, 1128, 1298
AluI	AGCT	8 cut(s)	13, 89, 247, 298, 405, 1007, 1128, 1298
Alw21I	GWGCWC	1 cut(s)	1517
Alw26I	GTCTC	4 cut(s)	550, 921, 1162, 1633
AlwI	GGATC	3 cut(s)	591, 1466, 1561
AlwNI	CAGNNNCTG	1 cut(s)	1085
Ama87I	CYCGRG	1 cut(s)	314
AoxI	GGCC	5 cut(s)	205, 472, 1276, 1412, 1580
ApeKI	GCWGC	5 cut(s)	89, 812, 1226, 1613, 1657
ApoI	RAATTY	3 cut(s)	532, 735, 1450
AseI	ATTAAT	1 cut(s)	936
AspLEI	GCGC	1 cut(s)	673
AspS9I	GGNCC	4 cut(s)	574, 1061, 1308, 1547
AsuHPI	GGTGA	1 cut(s)	754
AsuII	TTCGAA	2 cut(s)	1048, 1090
AvaI	CYCGRG	1 cut(s)	314
AvaII	GGWCC	4 cut(s)	574, 1061, 1308, 1547
BanI	GGYRCC	1 cut(s)	708
Bbv12I	GWGCWC	1 cut(s)	1517
BbvI	GCAGC	5 cut(s)	76, 799, 1213, 1600, 1644
BccI	CCATC	6 cut(s)	449, 464, 764, 800, 833, 1298
BcgI	CGANNNNNNTGC	2 cut(s)	656, 690
BciT130I	CCWGG	4 cut(s)	1020, 1065, 1551, 1584
BciVI	GTATCC	2 cut(s)	523, 1130
BcoDI	GTCTC	4 cut(s)	550, 921, 1162, 1633
BfaI	CTAG	2 cut(s)	834, 1563
BfmI	CTRYAG	4 cut(s)	90, 1123, 1177, 1227
BfuI	GTATCC	2 cut(s)	523, 1130
BisI	GCNGC	8 cut(s)	90, 208, 813, 1227, 1362, 1365, 1614, 1658
BlsI	GCNGC	8 cut(s)	91, 209, 814, 1228, 1363, 1366, 1615, 1659
BmcAI	AGTACT	1 cut(s)	911
Bme1390I	CCNGG	4 cut(s)	1020, 1065, 1551, 1584
Bme18I	GGWCC	4 cut(s)	574, 1061, 1308, 1547
BmeT110I	CYCGRG	1 cut(s)	314
BmgBI	CACGTC	1 cut(s)	462
BmgT120I	GGNCC	4 cut(s)	574, 1061, 1308, 1547
BmiI	GGNNCC	4 cut(s)	283, 575, 576, 710
BmrFI	CCNGG	4 cut(s)	1020, 1065, 1551, 1584
BmsI	GCATC	3 cut(s)	400, 790, 1600
Bpu14I	TTCGAA	2 cut(s)	1048, 1090
BpuEI	CTTGAG	1 cut(s)	1284
Bsa29I	ATCGAT	1 cut(s)	1470
BsaAI	YACGTR	1 cut(s)	886
BsaBI	GATNNNNATC	1 cut(s)	1317
BsaHI	GRCGYC	1 cut(s)	1376
BsaJI	CCNNGG	4 cut(s)	1019, 1273, 1362, 1415
Bsc4I	CCNNNNNNNGG	4 cut(s)	1070, 1274, 1667, 1682
Bse118I	RCCGGY	1 cut(s)	866
Bse1I	ACTGG	3 cut(s)	285, 610, 1687
Bse8I	GATNNNNATC	1 cut(s)	1317
BseBI	CCWGG	4 cut(s)	1020, 1065, 1551, 1584
BseCI	ATCGAT	1 cut(s)	1470
BseDI	CCNNGG	4 cut(s)	1019, 1273, 1362, 1415
BseJI	GATNNNNATC	1 cut(s)	1317
BseLI	CCNNNNNNNGG	4 cut(s)	1070, 1274, 1667, 1682
BseMII	CTCAG	5 cut(s)	118, 640, 1502, 1521, 1676
BseNI	ACTGG	3 cut(s)	285, 610, 1687
BseX3I	CGGCCG	1 cut(s)	205
BseXI	GCAGC	5 cut(s)	76, 799, 1213, 1600, 1644
BseYI	CCCAGC	1 cut(s)	401
BsgI	GTGCAG	2 cut(s)	696, 798
Bsh1236I	CGCG	3 cut(s)	673, 1364, 1490
Bsh1285I	CGRYCG	1 cut(s)	208
BshFI	GGCC	5 cut(s)	207, 474, 1278, 1414, 1582
BshNI	GGYRCC	1 cut(s)	708
BshVI	ATCGAT	1 cut(s)	1470
BsiEI	CGRYCG	1 cut(s)	208
BsiHKAI	GWGCWC	1 cut(s)	1517
BsiHKCI	CYCGRG	1 cut(s)	314
BsiSI	CCGG	2 cut(s)	723, 867
BslFI	GGGAC	3 cut(s)	560, 1251, 1345
BslI	CCNNNNNNNGG	4 cut(s)	1070, 1274, 1667, 1682
BsmAI	GTCTC	4 cut(s)	550, 921, 1162, 1633
BsmBI	CGTCTC	1 cut(s)	921
BsmFI	GGGAC	3 cut(s)	560, 1251, 1345
BsnI	GGCC	5 cut(s)	207, 474, 1278, 1414, 1582
BsoBI	CYCGRG	1 cut(s)	314
Bsp119I	TTCGAA	2 cut(s)	1048, 1090
Bsp1286I	GDGCHC	1 cut(s)	1517
Bsp1407I	TGTACA	1 cut(s)	537
Bsp143I	GATC	8 cut(s)	502, 583, 921, 945, 1467, 1471, 1553, 1596
Bsp19I	CCATGG	1 cut(s)	1415
BspACI	CCGC	7 cut(s)	119, 208, 390, 975, 1306, 1362, 1364
BspANI	GGCC	5 cut(s)	207, 474, 1278, 1414, 1582
BspCNI	CTCAG	5 cut(s)	117, 639, 1503, 1522, 1675
BspDI	ATCGAT	1 cut(s)	1470
BspFNI	CGCG	3 cut(s)	673, 1364, 1490
BspLI	GGNNCC	4 cut(s)	283, 575, 576, 710
BspMAI	CTGCAG	2 cut(s)	94, 1231
BspPI	GGATC	3 cut(s)	591, 1466, 1561
BspQI	GCTCTTC	1 cut(s)	20
BspT104I	TTCGAA	2 cut(s)	1048, 1090
BspT107I	GGYRCC	1 cut(s)	708
BsrFI	RCCGGY	1 cut(s)	866
BsrGI	TGTACA	1 cut(s)	537
BsrI	ACTGG	3 cut(s)	285, 610, 1687
BssAI	RCCGGY	1 cut(s)	866
BssECI	CCNNGG	4 cut(s)	1019, 1273, 1362, 1415
BssMI	GATC	8 cut(s)	502, 583, 921, 945, 1467, 1471, 1553, 1596
BssNI	GRCGYC	1 cut(s)	1376
BssT1I	CCWWGG	2 cut(s)	1273, 1415
Bst2UI	CCWGG	4 cut(s)	1020, 1065, 1551, 1584
Bst4CI	ACNGT	9 cut(s)	193, 376, 542, 932, 1045, 1204, 1393, 1438, 1627
Bst6I	CTCTTC	2 cut(s)	20, 873
BstACI	GRCGYC	1 cut(s)	1376
BstAPI	GCANNNNNTGC	1 cut(s)	1610
BstAUI	TGTACA	1 cut(s)	537
BstBAI	YACGTR	1 cut(s)	886
BstBI	TTCGAA	2 cut(s)	1048, 1090
BstC8I	GCNNGC	5 cut(s)	333, 675, 868, 1077, 1488
BstDEI	CTNAG	7 cut(s)	9, 104, 434, 626, 1511, 1530, 1662
BstDSI	CCRYGG	2 cut(s)	1362, 1415
BstEII	GGTNACC	1 cut(s)	1586
BstENI	CCTNNNNNAGG	1 cut(s)	1665
BstFNI	CGCG	3 cut(s)	673, 1364, 1490
BstHHI	GCGC	1 cut(s)	673
BstKTI	GATC	8 cut(s)	505, 586, 924, 948, 1470, 1474, 1556, 1599
BstMAI	GTCTC	4 cut(s)	550, 921, 1162, 1633
BstMBI	GATC	8 cut(s)	502, 583, 921, 945, 1467, 1471, 1553, 1596
BstMCI	CGRYCG	1 cut(s)	208
BstMWI	GCNNNNNNNGC	6 cut(s)	809, 972, 1004, 1013, 1373, 1610
BstNI	CCWGG	4 cut(s)	1020, 1065, 1551, 1584
BstPI	GGTNACC	1 cut(s)	1586
BstSCI	CCNGG	4 cut(s)	1018, 1063, 1549, 1582
BstSFI	CTRYAG	4 cut(s)	90, 1123, 1177, 1227
BstSNI	TACGTA	1 cut(s)	886
BstUI	CGCG	3 cut(s)	673, 1364, 1490
BstV1I	GCAGC	5 cut(s)	76, 799, 1213, 1600, 1644
BstZI	CGGCCG	1 cut(s)	205
Bsu15I	ATCGAT	1 cut(s)	1470
BsuI	GTATCC	2 cut(s)	523, 1130
BsuRI	GGCC	5 cut(s)	207, 474, 1278, 1414, 1582
BsuTUI	ATCGAT	1 cut(s)	1470
BtgI	CCRYGG	2 cut(s)	1362, 1415
BtrI	CACGTC	1 cut(s)	462
BtsI	GCAGTG	2 cut(s)	861, 1053
BtsIMutI	CAGTG	8 cut(s)	189, 547, 650, 861, 1053, 1389, 1508, 1680
Cac8I	GCNNGC	5 cut(s)	333, 675, 868, 1077, 1488
CaiI	CAGNNNCTG	1 cut(s)	1085
CfoI	GCGC	1 cut(s)	673
Cfr10I	RCCGGY	1 cut(s)	866
Cfr13I	GGNCC	4 cut(s)	574, 1061, 1308, 1547
Cfr42I	CCGCGG	1 cut(s)	1365
ClaI	ATCGAT	1 cut(s)	1470
CpoI	CGGWCCG	1 cut(s)	1308
CseI	GACGC	1 cut(s)	1365
Csp6I	GTAC	5 cut(s)	528, 538, 883, 910, 1534
CspI	CGGWCCG	1 cut(s)	1308
CviAII	CATG	5 cut(s)	1069, 1416, 1456, 1578, 1592
CviQI	GTAC	5 cut(s)	528, 538, 883, 910, 1534
DdeI	CTNAG	7 cut(s)	9, 104, 434, 626, 1511, 1530, 1662
DpnI	GATC	8 cut(s)	504, 585, 923, 947, 1469, 1473, 1555, 1598
DpnII	GATC	8 cut(s)	502, 583, 921, 945, 1467, 1471, 1553, 1596
EaeI	YGGCCR	1 cut(s)	205
EagI	CGGCCG	1 cut(s)	205
Eam1104I	CTCTTC	2 cut(s)	20, 873
EarI	CTCTTC	2 cut(s)	20, 873
EciI	GGCGGA	2 cut(s)	108, 1321
EclXI	CGGCCG	1 cut(s)	205
Eco105I	TACGTA	1 cut(s)	886
Eco130I	CCWWGG	2 cut(s)	1273, 1415
Eco47I	GGWCC	4 cut(s)	574, 1061, 1308, 1547
Eco52I	CGGCCG	1 cut(s)	205
Eco57I	CTGAAG	1 cut(s)	69
Eco88I	CYCGRG	1 cut(s)	314
Eco91I	GGTNACC	1 cut(s)	1586
EcoNI	CCTNNNNNAGG	1 cut(s)	1665
EcoO109I	RGGNCCY	2 cut(s)	574, 1547
EcoO65I	GGTNACC	1 cut(s)	1586
EcoRII	CCWGG	4 cut(s)	1018, 1063, 1549, 1582
EcoT14I	CCWWGG	2 cut(s)	1273, 1415
ErhI	CCWWGG	2 cut(s)	1273, 1415
Esp3I	CGTCTC	1 cut(s)	921
FaeI	CATG	5 cut(s)	1072, 1419, 1459, 1581, 1595
FalI	AAGNNNNNCTT	2 cut(s)	896, 928
FaqI	GGGAC	3 cut(s)	560, 1251, 1345
FatI	CATG	5 cut(s)	1068, 1415, 1455, 1577, 1591
FauNDI	CATATG	1 cut(s)	131
Fnu4HI	GCNGC	8 cut(s)	90, 208, 813, 1227, 1362, 1365, 1614, 1658
Fsp4HI	GCNGC	8 cut(s)	90, 208, 813, 1227, 1362, 1365, 1614, 1658
FspBI	CTAG	2 cut(s)	834, 1563
GlaI	GCGC	1 cut(s)	672
GluI	GCNGC	8 cut(s)	90, 208, 813, 1227, 1362, 1365, 1614, 1658
GsaI	CCCAGC	1 cut(s)	405
HaeIII	GGCC	5 cut(s)	207, 474, 1278, 1414, 1582
HapII	CCGG	2 cut(s)	723, 867
HgaI	GACGC	1 cut(s)	1365
HhaI	GCGC	1 cut(s)	673
Hin1I	GRCGYC	1 cut(s)	1376
Hin1II	CATG	5 cut(s)	1072, 1419, 1459, 1581, 1595
Hin6I	GCGC	1 cut(s)	671
HinP1I	GCGC	1 cut(s)	671
HincII	GTYRAC	1 cut(s)	379
HindII	GTYRAC	1 cut(s)	379
HindIII	AAGCTT	1 cut(s)	245
HinfI	GANTC	2 cut(s)	686, 1318
HpaI	GTTAAC	1 cut(s)	379
HpaII	CCGG	2 cut(s)	723, 867
HphI	GGTGA	1 cut(s)	754
Hpy166II	GTNNAC	4 cut(s)	141, 379, 682, 1441
Hpy188I	TCNGA	8 cut(s)	409, 469, 635, 926, 942, 1285, 1323, 1665
Hpy188III	TCNNGA	4 cut(s)	251, 368, 1342, 1396
Hpy8I	GTNNAC	4 cut(s)	141, 379, 682, 1441
Hpy99I	CGWCG	1 cut(s)	1266
HpyAV	CCTTC	7 cut(s)	162, 485, 699, 722, 739, 997, 1414
HpyCH4III	ACNGT	9 cut(s)	193, 376, 542, 932, 1045, 1204, 1393, 1438, 1627
HpyCH4IV	ACGT	2 cut(s)	461, 885
HpyF10VI	GCNNNNNNNGC	6 cut(s)	809, 972, 1004, 1013, 1373, 1610
HpyF3I	CTNAG	7 cut(s)	9, 104, 434, 626, 1511, 1530, 1662
HpySE526I	ACGT	2 cut(s)	461, 885
Hsp92I	GRCGYC	1 cut(s)	1376
Hsp92II	CATG	5 cut(s)	1072, 1419, 1459, 1581, 1595
HspAI	GCGC	1 cut(s)	671
KflI	GGGWCCC	1 cut(s)	574
KroI	GCCGGC	1 cut(s)	866
KroNI	GCCGGC	1 cut(s)	868
KspAI	GTTAAC	1 cut(s)	379
KspI	CCGCGG	1 cut(s)	1365
Kzo9I	GATC	8 cut(s)	502, 583, 921, 945, 1467, 1471, 1553, 1596
LguI	GCTCTTC	1 cut(s)	20
LmnI	GCTCC	1 cut(s)	410
Lsp1109I	GCAGC	5 cut(s)	76, 799, 1213, 1600, 1644
LweI	GCATC	3 cut(s)	400, 790, 1600
MaeI	CTAG	2 cut(s)	834, 1563
MaeII	ACGT	2 cut(s)	461, 885
MaeIII	GTNAC	7 cut(s)	559, 648, 859, 1187, 1382, 1573, 1586
MalI	GATC	8 cut(s)	504, 585, 923, 947, 1469, 1473, 1555, 1598
MboI	GATC	8 cut(s)	502, 583, 921, 945, 1467, 1471, 1553, 1596
MboII	GAAGA	6 cut(s)	7, 389, 707, 714, 743, 890
MhlI	GDGCHC	1 cut(s)	1517
MluCI	AATT	8 cut(s)	532, 735, 891, 1172, 1345, 1450, 1651, 1692
MlyI	GAGTC	1 cut(s)	680
MmeI	TCCRAC	3 cut(s)	244, 949, 1656
MroNI	GCCGGC	1 cut(s)	866
MseI	TTAA	5 cut(s)	341, 378, 446, 936, 1691
MslI	CAYNNNNRTG	1 cut(s)	183
MspA1I	CMGCKG	3 cut(s)	89, 298, 1364
MspI	CCGG	2 cut(s)	723, 867
MspR9I	CCNGG	4 cut(s)	1020, 1065, 1551, 1584
MvaI	CCWGG	4 cut(s)	1020, 1065, 1551, 1584
MvnI	CGCG	3 cut(s)	673, 1364, 1490
MwoI	GCNNNNNNNGC	6 cut(s)	809, 972, 1004, 1013, 1373, 1610
NaeI	GCCGGC	1 cut(s)	868
NcoI	CCATGG	1 cut(s)	1415
NdeI	CATATG	1 cut(s)	131
NdeII	GATC	8 cut(s)	502, 583, 921, 945, 1467, 1471, 1553, 1596
NgoMIV	GCCGGC	1 cut(s)	866
NlaIII	CATG	5 cut(s)	1072, 1419, 1459, 1581, 1595
NlaIV	GGNNCC	4 cut(s)	283, 575, 576, 710
NmuCI	GTSAC	3 cut(s)	648, 859, 1187
NspV	TTCGAA	2 cut(s)	1048, 1090
PaeR7I	CTCGAG	1 cut(s)	314
PciSI	GCTCTTC	1 cut(s)	20
PdiI	GCCGGC	1 cut(s)	868
PfeI	GAWTC	1 cut(s)	1318
PflFI	GACNNNGTC	1 cut(s)	419
PkrI	GCNGC	8 cut(s)	91, 209, 814, 1228, 1363, 1366, 1615, 1659
PleI	GAGTC	1 cut(s)	680
PpsI	GAGTC	1 cut(s)	680
Ppu21I	YACGTR	1 cut(s)	886
PpuMI	RGGWCCY	2 cut(s)	574, 1547
PshBI	ATTAAT	1 cut(s)	936
PsiI	TTATAA	1 cut(s)	695
Psp5II	RGGWCCY	2 cut(s)	574, 1547
Psp6I	CCWGG	4 cut(s)	1018, 1063, 1549, 1582
PspEI	GGTNACC	1 cut(s)	1586
PspFI	CCCAGC	1 cut(s)	401
PspGI	CCWGG	4 cut(s)	1018, 1063, 1549, 1582
PspN4I	GGNNCC	4 cut(s)	283, 575, 576, 710
PspPI	GGNCC	4 cut(s)	574, 1061, 1308, 1547
PspPPI	RGGWCCY	2 cut(s)	574, 1547
PspXI	VCTCGAGB	1 cut(s)	314
PstI	CTGCAG	2 cut(s)	94, 1231
PstNI	CAGNNNCTG	1 cut(s)	1085
PsyI	GACNNNGTC	1 cut(s)	419
PvuII	CAGCTG	2 cut(s)	89, 298
RsaI	GTAC	5 cut(s)	529, 539, 884, 911, 1535
RsaNI	GTAC	5 cut(s)	528, 538, 883, 910, 1534
RseI	CAYNNNNRTG	1 cut(s)	183
Rsr2I	CGGWCCG	1 cut(s)	1308
RsrII	CGGWCCG	1 cut(s)	1308
SacII	CCGCGG	1 cut(s)	1365
SapI	GCTCTTC	1 cut(s)	20
SaqAI	TTAA	5 cut(s)	341, 378, 446, 936, 1691
SatI	GCNGC	8 cut(s)	90, 208, 813, 1227, 1362, 1365, 1614, 1658
Sau3AI	GATC	8 cut(s)	502, 583, 921, 945, 1467, 1471, 1553, 1596
Sau96I	GGNCC	4 cut(s)	574, 1061, 1308, 1547
ScaI	AGTACT	1 cut(s)	911
SchI	GAGTC	1 cut(s)	680
ScrFI	CCNGG	4 cut(s)	1020, 1065, 1551, 1584
SduI	GDGCHC	1 cut(s)	1517
SfaNI	GCATC	3 cut(s)	400, 790, 1600
SfcI	CTRYAG	4 cut(s)	90, 1123, 1177, 1227
Sfr274I	CTCGAG	1 cut(s)	314
Sfr303I	CCGCGG	1 cut(s)	1365
SfuI	TTCGAA	2 cut(s)	1048, 1090
SgrBI	CCGCGG	1 cut(s)	1365
SinI	GGWCC	4 cut(s)	574, 1061, 1308, 1547
SlaI	CTCGAG	1 cut(s)	314
SmiMI	CAYNNNNRTG	1 cut(s)	183
SmlI	CTYRAG	2 cut(s)	314, 1299
SmoI	CTYRAG	2 cut(s)	314, 1299
SnaBI	TACGTA	1 cut(s)	886
Sse9I	AATT	8 cut(s)	532, 735, 891, 1172, 1345, 1450, 1651, 1692
SsiI	CCGC	7 cut(s)	119, 208, 390, 975, 1306, 1362, 1364
SspMI	CTAG	2 cut(s)	834, 1563
StyD4I	CCNGG	4 cut(s)	1018, 1063, 1549, 1582
StyI	CCWWGG	2 cut(s)	1273, 1415
TaaI	ACNGT	9 cut(s)	193, 376, 542, 932, 1045, 1204, 1393, 1438, 1627
TaiI	ACGT	2 cut(s)	464, 888
TaqI	TCGA	8 cut(s)	315, 614, 700, 899, 1048, 1090, 1430, 1470
TaqII	GACCGA	1 cut(s)	1325
TasI	AATT	8 cut(s)	532, 735, 891, 1172, 1345, 1450, 1651, 1692
TatI	WGTACW	3 cut(s)	537, 909, 1533
TauI	GCSGC	3 cut(s)	210, 1364, 1367
TfiI	GAWTC	1 cut(s)	1318
Tru1I	TTAA	5 cut(s)	341, 378, 446, 936, 1691
Tru9I	TTAA	5 cut(s)	341, 378, 446, 936, 1691
TscAI	CASTG	8 cut(s)	196, 547, 657, 868, 1060, 1396, 1515, 1687
TseFI	GTSAC	3 cut(s)	648, 859, 1187
TseI	GCWGC	5 cut(s)	89, 812, 1226, 1613, 1657
Tsp45I	GTSAC	3 cut(s)	648, 859, 1187
TspDTI	ATGAA	6 cut(s)	24, 744, 891, 1201, 1444, 1627
TspGWI	ACGGA	3 cut(s)	157, 505, 1212
TspRI	CASTG	8 cut(s)	196, 547, 657, 868, 1060, 1396, 1515, 1687
Tth111I	GACNNNGTC	1 cut(s)	419
VpaK11BI	GGWCC	4 cut(s)	574, 1061, 1308, 1547
VspI	ATTAAT	1 cut(s)	936
XagI	CCTNNNNNAGG	1 cut(s)	1665
XapI	RAATTY	3 cut(s)	532, 735, 1450
XcmI	CCANNNNNNNNNTGG	3 cut(s)	773, 1006, 1639
XhoI	CTCGAG	1 cut(s)	314
XspI	CTAG	2 cut(s)	834, 1563
ZrmI	AGTACT	1 cut(s)	911

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.