FvH4_5g35580

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000522
Physical Location & Seq
Reverse (-)
26047250 .. 26049406
2157 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35580.t1

Sequence Viewer

Length: 1701 bp
ATGGCTTCTAAGCTCTTCAATCCTCTTGTAACTTCATCACTATCACTACTATTCCTCATCATTTTCGCTTTGTTCATATCTCAACCTTCAGCGGCAGATGTTCCTCTCAACACTTCTGTTCCGCCTGAAACCATATGCAAGTCCACTCCACACCCTTCTGTCTGCACTTCCGTCCTCCCTCACAAAAACGCCAATGTCTATGACTTCGGCCGGTTTTCAGTCCAATACTCCTTCGTACAATCCCAAAAGCTTCTGGATTTGATCAACAAGTATCTTCAAGGGGGTTCCAGTTTATCTCAACTGGAAATCCAAGCCCTCGAAGATTGCAAGTCCCTTGCATTGTTAAATATAGATTTCTTATCAAACGCTCTTGAAACTGTTAACAAGACGAGCGACGTTCTTCCGAGCTCCGATGCCGATGATGTCCAAACCCTCCTCAGTGCCATCTTGACCAACCAGCAGACATGTTTTGATGGCCTTCAATCTGTGAGTTCAGTCCAAAATGATCTCTCAGTGTCTGTCTCTAATGGTACAAACTTTTACAGTGTCTCATTGGCGTTGGTTACAAAGGGTTGGGTGCCAAAGGGTCACAAAAGTAATGGAACACCAAACCAGTCGAGAAAGCATCTCAGTTTCCGAGAGGGGCGATTGTCACTGAAAATGTCAAGCAAAGCTCGTGCAGTTTACGACTCAGTTATCAATCGAAGAAGGCACCTTCTTCAGGCCGGAGATGAAGAAGTTGTGGTGAAGGACATTGTGGTTGTGAGCCAAGATGGAACCGGAAACTTCACTACCATCAATGATGCTATCAATGCTGCCCCCAATAACACTGTTGCAAGTGATGGATACTTCTTGGTATATATCACTGCCGGCGTTTATGAGGAGTATGTATCAATTATATCGAAAAAGAAGTACTTGTTGCTGATCGGAGACGGTATTAATCAGACAATCATCACCGGAAGCAACAGTGTCGGAGGCGGTTCAACCACCTTCAACTCTGCCACTTTAGCTGTGGTAGCAACAGGGTTTATTGCAGTGAACATAACTGTTCGAAACACTGCTGGACCAAGCATGGGGCAAGCAGTTGCAGTTCGAAACGGCGCCGACTTGTCAGTTTTCTACAGCTGCAGCTTTGAGGGATACCAAGACACCTTATACACACATTCTATGAGACAATTCTACAGAGAATGTGACATCTACGGAACGGTTGATTTCATATTCGGAAATGCTGCAGTTGTTTTCCAAAACTGCAACATATACCCTCGCCAGCCCCGACAAGGCCAGACGAATCCTATCACAGCGCAGGGCCGAACAGACCCGAATCAGAACACAGGGACTTCAATCCAGAATTGCACCATTACAGCCGCAGATGATTTGGCTTCGAGTAACTTCACTGTCAAGACATATCTAGGGAGGCCATGGAAGGAGTACTCGAGGACGGTTTACTTGCAGAATTTCATGGATAGTTTGATTGATCCTGCTGGCTGGCTAGCATGGAGTGGAGATTTTGCACTGAGCACATTGTATTATGGTGAGTATGATAATAAAGGACCCGGAGCAGACACTAGTAACAGAGTTACATGGCCTGGTTACCATATAATCGGTGCAACTGATGCTTCAAATTTTACAGTGTCAAACTTCGTGTTGGGGGATAGTTGGTTGCCTCAAACAGGAGTGCCTTACACCGGTGGCTTAATGTGA

Protein Analysis

567

Amino Acids

61.41

Weight (kDa)

5.55

Isoelectric Point (pI)

34.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 43 - 187 3.8e-25 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 253 - 551 1.7e-136 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000522)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02810 AT2G47550 AT4G02330
fragaria_vesca FvH4_2g25960 FvH4_2g25960 FvH4_2g25970 FvH4_5g35570 FvH4_5g35580 FvH4_5g35590
malus_domestica MD03G1290900.v1.1 MD03G1291000.v1.1 MD08G1195600.v1.1 MD11G1307400.v1.1 MD11G1307500.v1.1
prunus_persica Prupe.1G529400_v2.0.a1 Prupe.1G529500_v2.0.a1 Prupe.8G263900_v2.0.a1 Prupe.8G264000_v2.0.a1
pyrus_communis pycom08g16800
rosa_chinensis RchiOBHm_Chr6g0294321 RchiOBHm_Chr6g0294331 RchiOBHm_Chr7g0236951 RchiOBHm_Chr7g0236971 RchiOBHm_Chr7g0237041 RchiOBHm_Chr7g0237051
rosa_laevigata RLG00000001034 RLG00000001035 RLG00000001039 RLG00000001043 RLG00000001045 RLG00000011880 RLG00000011881 RLG00000034181
rosa_multiflora Rmu_co8086188.1_g000001 Rmu_co8458485.1_g000001 Rmu_sc0000272.1_g000007 Rmu_sc0000272.1_g000009 Rmu_sc0002131.1_g000030 Rmu_sc0002131.1_g000037 Rmu_sc0005369.1_g000005 Rmu_sc0005369.1_g000008 Rmu_sc0007991.1_g000005 Rmu_sc0007991.1_g000013 Rmu_sc0033563.1_g000001
rosa_roxburghii Rroxscaffold_3G00224540 Rroxscaffold_3G00224600 Rroxscaffold_3G00224650 Rroxscaffold_3G00224660 Rroxscaffold_7G00173470 Rroxscaffold_7G00173480
rosa_rugosa Rorug06G0243400 Rorug06G0243500 Rorug07G0301200 Rorug07G0301500 Rorug07G0301600
rosa_samantha Rh6AG356000 Rh6AG356100 Rh6BG362600 Rh6BG362800 Rh6CG370000 Rh6CG370100 Rh6DG356500 Rh6DG356600 Rh7AG456200 Rh7AG456500 Rh7AG457500 Rh7AG457600 Rh7BG427500 Rh7BG427700 Rh7BG428400 Rh7BG428500 Rh7CG475100 Rh7CG475300 Rh7CG475700 Rh7CG475800 Rh7DG444100 Rh7DG444300 Rh7DG444700 Rh7DG444900
rosa_wichuraiana Rw6G031020 Rw6G031030 Rw7G037870 Rw7G037880 Rw7G037920 Rw7G037940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 577, 711, 1100
AciI CCGC 4 cut(s) 92, 122, 978, 1365
AclWI GGATC 1 cut(s) 1469
AcoI YGGCCR 1 cut(s) 208
AcsI RAATTY 2 cut(s) 1453, 1621
AcuI CTGAAG 2 cut(s) 72, 704
AcyI GRCGYC 1 cut(s) 1101
AfaI GTAC 4 cut(s) 237, 532, 914, 1430
AfiI CCNNNNNNNGG 5 cut(s) 721, 1073, 1277, 1670, 1685
AflIII ACRYGT 1 cut(s) 464
AgeI ACCGGT 1 cut(s) 1685
AgsI TTSAA 8 cut(s) 19, 278, 374, 482, 984, 994, 1341, 1620
AhlI ACTAGT 1 cut(s) 1565
AjnI CCWGG 1 cut(s) 1585
AluBI AGCT 7 cut(s) 13, 250, 408, 674, 1010, 1125, 1131
AluI AGCT 7 cut(s) 13, 250, 408, 674, 1010, 1125, 1131
Alw21I GWGCWC 2 cut(s) 410, 1520
Alw26I GTCTC 4 cut(s) 526, 553, 924, 1165
AlwI GGATC 1 cut(s) 1469
AlwNI CAGNNNCTG 1 cut(s) 518
Ama87I CYCGRG 1 cut(s) 1432
AoxI GGCC 7 cut(s) 208, 475, 723, 1279, 1306, 1415, 1583
ApeKI GCWGC 4 cut(s) 815, 1125, 1128, 1229
ApoI RAATTY 2 cut(s) 1453, 1621
AseI ATTAAT 1 cut(s) 939
AsiGI ACCGGT 1 cut(s) 1685
AspLEI GCGC 2 cut(s) 1103, 1303
AspS9I GGNCC 3 cut(s) 1064, 1306, 1550
AsuC2I CCSGG 1 cut(s) 1554
AsuHPI GGTGA 3 cut(s) 757, 946, 1544
AsuII TTCGAA 2 cut(s) 1051, 1093
AsuNHI GCTAGC 1 cut(s) 1489
AvaI CYCGRG 1 cut(s) 1432
AvaII GGWCC 2 cut(s) 1064, 1550
BanI GGYRCC 3 cut(s) 577, 711, 1100
BanII GRGCYC 1 cut(s) 410
BauI CACGAG 1 cut(s) 675
Bbv12I GWGCWC 2 cut(s) 410, 1520
BbvI GCAGC 4 cut(s) 802, 1112, 1140, 1216
BccI CCATC 5 cut(s) 452, 467, 767, 803, 836
BceAI ACGGC 1 cut(s) 1114
BcgI CGANNNNNNTGC 2 cut(s) 952, 986
BciT130I CCWGG 1 cut(s) 1587
BciVI GTATCC 2 cut(s) 839, 1133
BclI TGATCA 1 cut(s) 261
BcnI CCSGG 1 cut(s) 1554
BcoDI GTCTC 4 cut(s) 526, 553, 924, 1165
BcuI ACTAGT 1 cut(s) 1565
BfaI CTAG 3 cut(s) 1409, 1490, 1566
BfmI CTRYAG 4 cut(s) 1120, 1126, 1180, 1230
BfoI RGCGCY 1 cut(s) 1104
BfuI GTATCC 2 cut(s) 839, 1133
BisI GCNGC 6 cut(s) 93, 816, 1126, 1129, 1230, 1365
BlsI GCNGC 6 cut(s) 94, 817, 1127, 1130, 1231, 1366
BmcAI AGTACT 2 cut(s) 914, 1430
Bme1390I CCNGG 2 cut(s) 1554, 1587
Bme18I GGWCC 2 cut(s) 1064, 1550
BmeT110I CYCGRG 1 cut(s) 1432
BmgT120I GGNCC 3 cut(s) 1064, 1306, 1550
BmiI GGNNCC 6 cut(s) 286, 579, 713, 778, 1102, 1552
BmrFI CCNGG 2 cut(s) 1554, 1587
BmsI GCATC 4 cut(s) 403, 634, 793, 1603
BmtI GCTAGC 1 cut(s) 1493
Bpu14I TTCGAA 2 cut(s) 1051, 1093
BpuMI CCSGG 1 cut(s) 1554
BsaHI GRCGYC 1 cut(s) 1101
BsaJI CCNNGG 1 cut(s) 1418
BsaWI WCCGGW 3 cut(s) 779, 956, 1685
Bsc4I CCNNNNNNNGG 5 cut(s) 721, 1073, 1277, 1670, 1685
Bse118I RCCGGY 3 cut(s) 210, 869, 1685
Bse1I ACTGG 3 cut(s) 288, 306, 613
BseBI CCWGG 1 cut(s) 1587
BseDI CCNNGG 1 cut(s) 1418
BseLI CCNNNNNNNGG 5 cut(s) 721, 1073, 1277, 1670, 1685
BseMII CTCAG 5 cut(s) 451, 525, 643, 705, 1505
BseNI ACTGG 3 cut(s) 288, 306, 613
BseRI GAGGAG 2 cut(s) 425, 896
BseX3I CGGCCG 1 cut(s) 208
BseXI GCAGC 4 cut(s) 802, 1112, 1140, 1216
BsgI GTGCAG 2 cut(s) 148, 699
Bsh1285I CGRYCG 1 cut(s) 211
BshFI GGCC 7 cut(s) 210, 477, 725, 1281, 1308, 1417, 1585
BshNI GGYRCC 3 cut(s) 577, 711, 1100
BshTI ACCGGT 1 cut(s) 1685
BsiEI CGRYCG 1 cut(s) 211
BsiHKAI GWGCWC 2 cut(s) 410, 1520
BsiHKCI CYCGRG 1 cut(s) 1432
BsiSI CCGG 7 cut(s) 211, 726, 780, 870, 957, 1554, 1686
BslFI GGGAC 2 cut(s) 316, 1348
BslI CCNNNNNNNGG 5 cut(s) 721, 1073, 1277, 1670, 1685
BsmAI GTCTC 4 cut(s) 526, 553, 924, 1165
BsmBI CGTCTC 1 cut(s) 924
BsmFI GGGAC 2 cut(s) 316, 1348
BsnI GGCC 7 cut(s) 210, 477, 725, 1281, 1308, 1417, 1585
BsoBI CYCGRG 1 cut(s) 1432
Bsp119I TTCGAA 2 cut(s) 1051, 1093
Bsp1286I GDGCHC 2 cut(s) 410, 1520
Bsp143I GATC 4 cut(s) 261, 505, 924, 1474
Bsp19I CCATGG 1 cut(s) 1418
BspACI CCGC 4 cut(s) 92, 122, 978, 1365
BspANI GGCC 7 cut(s) 210, 477, 725, 1281, 1308, 1417, 1585
BspCNI CTCAG 5 cut(s) 450, 524, 642, 704, 1506
BspLI GGNNCC 6 cut(s) 286, 579, 713, 778, 1102, 1552
BspMAI CTGCAG 2 cut(s) 1130, 1234
BspOI GCTAGC 1 cut(s) 1493
BspPI GGATC 1 cut(s) 1469
BspQI GCTCTTC 1 cut(s) 20
BspT104I TTCGAA 2 cut(s) 1051, 1093
BspT107I GGYRCC 3 cut(s) 577, 711, 1100
BsrFI RCCGGY 3 cut(s) 210, 869, 1685
BsrI ACTGG 3 cut(s) 288, 306, 613
BssAI RCCGGY 3 cut(s) 210, 869, 1685
BssECI CCNNGG 1 cut(s) 1418
BssMI GATC 4 cut(s) 261, 505, 924, 1474
BssNI GRCGYC 1 cut(s) 1101
BssSI CACGAG 1 cut(s) 675
BssT1I CCWWGG 1 cut(s) 1418
Bst2BI CACGAG 1 cut(s) 675
Bst2UI CCWGG 1 cut(s) 1587
Bst6I CTCTTC 1 cut(s) 20
BstACI GRCGYC 1 cut(s) 1101
BstAPI GCANNNNNTGC 1 cut(s) 1613
BstBI TTCGAA 2 cut(s) 1051, 1093
BstC8I GCNNGC 6 cut(s) 871, 1080, 1268, 1483, 1487, 1491
BstDEI CTNAG 6 cut(s) 9, 437, 511, 629, 691, 1514
BstDSI CCRYGG 1 cut(s) 1418
BstEII GGTNACC 1 cut(s) 1589
BstENI CCTNNNNNAGG 2 cut(s) 719, 1668
BstH2I RGCGCY 1 cut(s) 1104
BstHHI GCGC 2 cut(s) 1103, 1303
BstKTI GATC 4 cut(s) 264, 508, 927, 1477
BstMAI GTCTC 4 cut(s) 526, 553, 924, 1165
BstMBI GATC 4 cut(s) 261, 505, 924, 1474
BstMCI CGRYCG 1 cut(s) 211
BstMWI GCNNNNNNNGC 4 cut(s) 812, 1007, 1016, 1613
BstNI CCWGG 1 cut(s) 1587
BstNSI RCATGY 1 cut(s) 468
BstPI GGTNACC 1 cut(s) 1589
BstSCI CCNGG 2 cut(s) 1552, 1585
BstSFI CTRYAG 4 cut(s) 1120, 1126, 1180, 1230
BstV1I GCAGC 4 cut(s) 802, 1112, 1140, 1216
BstZI CGGCCG 1 cut(s) 208
BsuI GTATCC 2 cut(s) 839, 1133
BsuRI GGCC 7 cut(s) 210, 477, 725, 1281, 1308, 1417, 1585
BtgI CCRYGG 1 cut(s) 1418
BtsI GCAGTG 3 cut(s) 864, 1041, 1056
Cac8I GCNNGC 6 cut(s) 871, 1080, 1268, 1483, 1487, 1491
CaiI CAGNNNCTG 1 cut(s) 518
CfoI GCGC 2 cut(s) 1103, 1303
Cfr10I RCCGGY 3 cut(s) 210, 869, 1685
Cfr13I GGNCC 3 cut(s) 1064, 1306, 1550
Csp6I GTAC 4 cut(s) 236, 531, 913, 1429
CspAI ACCGGT 1 cut(s) 1685
CviAII CATG 6 cut(s) 465, 1072, 1419, 1459, 1494, 1581
CviQI GTAC 4 cut(s) 236, 531, 913, 1429
DdeI CTNAG 6 cut(s) 9, 437, 511, 629, 691, 1514
DinI GGCGCC 1 cut(s) 1102
DpnI GATC 4 cut(s) 263, 507, 926, 1476
DpnII GATC 4 cut(s) 261, 505, 924, 1474
EaeI YGGCCR 1 cut(s) 208
EagI CGGCCG 1 cut(s) 208
Eam1104I CTCTTC 1 cut(s) 20
EarI CTCTTC 1 cut(s) 20
EciI GGCGGA 1 cut(s) 111
Ecl136II GAGCTC 1 cut(s) 408
EclXI CGGCCG 1 cut(s) 208
Eco130I CCWWGG 1 cut(s) 1418
Eco24I GRGCYC 1 cut(s) 410
Eco47I GGWCC 2 cut(s) 1064, 1550
Eco52I CGGCCG 1 cut(s) 208
Eco53kI GAGCTC 1 cut(s) 408
Eco57I CTGAAG 2 cut(s) 72, 704
Eco88I CYCGRG 1 cut(s) 1432
Eco91I GGTNACC 1 cut(s) 1589
EcoICRI GAGCTC 1 cut(s) 408
EcoNI CCTNNNNNAGG 2 cut(s) 719, 1668
EcoO109I RGGNCCY 1 cut(s) 1550
EcoO65I GGTNACC 1 cut(s) 1589
EcoRII CCWGG 1 cut(s) 1585
EcoT14I CCWWGG 1 cut(s) 1418
EcoT38I GRGCYC 1 cut(s) 410
EgeI GGCGCC 1 cut(s) 1102
EheI GGCGCC 1 cut(s) 1102
ErhI CCWWGG 1 cut(s) 1418
Esp3I CGTCTC 1 cut(s) 924
FaeI CATG 6 cut(s) 468, 1075, 1422, 1462, 1497, 1584
FalI AAGNNNNNCTT 2 cut(s) 899, 931
FaqI GGGAC 2 cut(s) 316, 1348
FatI CATG 6 cut(s) 464, 1071, 1418, 1458, 1493, 1580
FauNDI CATATG 1 cut(s) 134
FbaI TGATCA 1 cut(s) 261
Fnu4HI GCNGC 6 cut(s) 93, 816, 1126, 1129, 1230, 1365
FriOI GRGCYC 1 cut(s) 410
Fsp4HI GCNGC 6 cut(s) 93, 816, 1126, 1129, 1230, 1365
FspBI CTAG 3 cut(s) 1409, 1490, 1566
GlaI GCGC 2 cut(s) 1102, 1302
GluI GCNGC 6 cut(s) 93, 816, 1126, 1129, 1230, 1365
HaeII RGCGCY 1 cut(s) 1104
HaeIII GGCC 7 cut(s) 210, 477, 725, 1281, 1308, 1417, 1585
HapII CCGG 7 cut(s) 211, 726, 780, 870, 957, 1554, 1686
HhaI GCGC 2 cut(s) 1103, 1303
Hin1I GRCGYC 1 cut(s) 1101
Hin1II CATG 6 cut(s) 468, 1075, 1422, 1462, 1497, 1584
Hin6I GCGC 2 cut(s) 1101, 1301
HinP1I GCGC 2 cut(s) 1101, 1301
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HindIII AAGCTT 1 cut(s) 248
HinfI GANTC 3 cut(s) 689, 1288, 1321
HpaI GTTAAC 1 cut(s) 382
HpaII CCGG 7 cut(s) 211, 726, 780, 870, 957, 1554, 1686
HphI GGTGA 3 cut(s) 757, 946, 1544
Hpy166II GTNNAC 5 cut(s) 144, 382, 685, 1039, 1444
Hpy188I TCNGA 8 cut(s) 405, 412, 638, 929, 945, 974, 1223, 1326
Hpy188III TCNNGA 6 cut(s) 254, 371, 448, 618, 1345, 1399
Hpy8I GTNNAC 5 cut(s) 144, 382, 685, 1039, 1444
Hpy99I CGWCG 1 cut(s) 398
HpyAV CCTTC 9 cut(s) 96, 165, 241, 488, 702, 725, 742, 1000, 1417
HpyCH4IV ACGT 1 cut(s) 396
HpyF10VI GCNNNNNNNGC 4 cut(s) 812, 1007, 1016, 1613
HpyF3I CTNAG 6 cut(s) 9, 437, 511, 629, 691, 1514
HpySE526I ACGT 1 cut(s) 396
Hsp92I GRCGYC 1 cut(s) 1101
Hsp92II CATG 6 cut(s) 468, 1075, 1422, 1462, 1497, 1584
HspAI GCGC 2 cut(s) 1101, 1301
KasI GGCGCC 1 cut(s) 1100
KroI GCCGGC 1 cut(s) 869
KroNI GCCGGC 1 cut(s) 871
Ksp22I TGATCA 1 cut(s) 261
KspAI GTTAAC 1 cut(s) 382
Kzo9I GATC 4 cut(s) 261, 505, 924, 1474
LguI GCTCTTC 1 cut(s) 20
LmnI GCTCC 2 cut(s) 413, 1556
Lsp1109I GCAGC 4 cut(s) 802, 1112, 1140, 1216
LweI GCATC 4 cut(s) 403, 634, 793, 1603
MaeI CTAG 3 cut(s) 1409, 1490, 1566
MaeII ACGT 1 cut(s) 396
MaeIII GTNAC 9 cut(s) 28, 562, 587, 651, 1190, 1385, 1568, 1576, 1589
MalI GATC 4 cut(s) 263, 507, 926, 1476
MboI GATC 4 cut(s) 261, 505, 924, 1474
MboII GAAGA 7 cut(s) 7, 266, 332, 392, 710, 717, 746
MhlI GDGCHC 2 cut(s) 410, 1520
MluCI AATT 5 cut(s) 894, 1175, 1348, 1453, 1621
Mly113I GGCGCC 1 cut(s) 1101
MlyI GAGTC 1 cut(s) 683
MmeI TCCRAC 1 cut(s) 952
MroNI GCCGGC 1 cut(s) 869
MseI TTAA 4 cut(s) 344, 381, 939, 1694
MspA1I CMGCKG 2 cut(s) 92, 1125
MspI CCGG 7 cut(s) 211, 726, 780, 870, 957, 1554, 1686
MspR9I CCNGG 2 cut(s) 1554, 1587
MvaI CCWGG 1 cut(s) 1587
MwoI GCNNNNNNNGC 4 cut(s) 812, 1007, 1016, 1613
NaeI GCCGGC 1 cut(s) 871
NarI GGCGCC 1 cut(s) 1101
NciI CCSGG 1 cut(s) 1554
NcoI CCATGG 1 cut(s) 1418
NdeI CATATG 1 cut(s) 134
NdeII GATC 4 cut(s) 261, 505, 924, 1474
NgoMIV GCCGGC 1 cut(s) 869
NheI GCTAGC 1 cut(s) 1489
NlaIII CATG 6 cut(s) 468, 1075, 1422, 1462, 1497, 1584
NlaIV GGNNCC 6 cut(s) 286, 579, 713, 778, 1102, 1552
NmuCI GTSAC 3 cut(s) 587, 651, 1190
NspI RCATGY 1 cut(s) 468
NspV TTCGAA 2 cut(s) 1051, 1093
PaeR7I CTCGAG 1 cut(s) 1432
PciI ACATGT 1 cut(s) 464
PciSI GCTCTTC 1 cut(s) 20
PcsI WCGNNNNNNNCGW 1 cut(s) 1270
PdiI GCCGGC 1 cut(s) 871
PfeI GAWTC 2 cut(s) 1288, 1321
PinAI ACCGGT 1 cut(s) 1685
PkrI GCNGC 6 cut(s) 94, 817, 1127, 1130, 1231, 1366
PleI GAGTC 1 cut(s) 683
PluTI GGCGCC 1 cut(s) 1104
PpsI GAGTC 1 cut(s) 683
PpuMI RGGWCCY 1 cut(s) 1550
PscI ACATGT 1 cut(s) 464
PshBI ATTAAT 1 cut(s) 939
Psp124BI GAGCTC 1 cut(s) 410
Psp5II RGGWCCY 1 cut(s) 1550
Psp6I CCWGG 1 cut(s) 1585
PspEI GGTNACC 1 cut(s) 1589
PspGI CCWGG 1 cut(s) 1585
PspN4I GGNNCC 6 cut(s) 286, 579, 713, 778, 1102, 1552
PspPI GGNCC 3 cut(s) 1064, 1306, 1550
PspPPI RGGWCCY 1 cut(s) 1550
PspXI VCTCGAGB 1 cut(s) 1432
PstI CTGCAG 2 cut(s) 1130, 1234
PstNI CAGNNNCTG 1 cut(s) 518
PvuII CAGCTG 1 cut(s) 1125
RsaI GTAC 4 cut(s) 237, 532, 914, 1430
RsaNI GTAC 4 cut(s) 236, 531, 913, 1429
SacI GAGCTC 1 cut(s) 410
SapI GCTCTTC 1 cut(s) 20
SaqAI TTAA 4 cut(s) 344, 381, 939, 1694
SatI GCNGC 6 cut(s) 93, 816, 1126, 1129, 1230, 1365
Sau3AI GATC 4 cut(s) 261, 505, 924, 1474
Sau96I GGNCC 3 cut(s) 1064, 1306, 1550
ScaI AGTACT 2 cut(s) 914, 1430
SchI GAGTC 1 cut(s) 683
ScrFI CCNGG 2 cut(s) 1554, 1587
SduI GDGCHC 2 cut(s) 410, 1520
SfaNI GCATC 4 cut(s) 403, 634, 793, 1603
SfcI CTRYAG 4 cut(s) 1120, 1126, 1180, 1230
SfoI GGCGCC 1 cut(s) 1102
Sfr274I CTCGAG 1 cut(s) 1432
SfuI TTCGAA 2 cut(s) 1051, 1093
SgrAI CRCCGGYG 1 cut(s) 1685
SinI GGWCC 2 cut(s) 1064, 1550
SlaI CTCGAG 1 cut(s) 1432
SmlI CTYRAG 1 cut(s) 1432
SmoI CTYRAG 1 cut(s) 1432
SpeI ACTAGT 1 cut(s) 1565
Sse9I AATT 5 cut(s) 894, 1175, 1348, 1453, 1621
SsiI CCGC 4 cut(s) 92, 122, 978, 1365
SspDI GGCGCC 1 cut(s) 1100
SspMI CTAG 3 cut(s) 1409, 1490, 1566
SstI GAGCTC 1 cut(s) 410
StyD4I CCNGG 2 cut(s) 1552, 1585
StyI CCWWGG 1 cut(s) 1418
TaiI ACGT 1 cut(s) 399
TaqI TCGA 8 cut(s) 318, 617, 703, 902, 1051, 1093, 1382, 1433
TasI AATT 5 cut(s) 894, 1175, 1348, 1453, 1621
TatI WGTACW 2 cut(s) 912, 1428
TauI GCSGC 2 cut(s) 95, 1367
TfiI GAWTC 2 cut(s) 1288, 1321
Tru1I TTAA 4 cut(s) 344, 381, 939, 1694
Tru9I TTAA 4 cut(s) 344, 381, 939, 1694
TseFI GTSAC 3 cut(s) 587, 651, 1190
TseI GCWGC 4 cut(s) 815, 1125, 1128, 1229
Tsp45I GTSAC 3 cut(s) 587, 651, 1190
TspDTI ATGAA 5 cut(s) 24, 64, 747, 1204, 1447
TspGWI ACGGA 2 cut(s) 160, 1215
VpaK11BI GGWCC 2 cut(s) 1064, 1550
VspI ATTAAT 1 cut(s) 939
XagI CCTNNNNNAGG 2 cut(s) 719, 1668
XapI RAATTY 2 cut(s) 1453, 1621
XceI RCATGY 1 cut(s) 468
XcmI CCANNNNNNNNNTGG 1 cut(s) 1009
XhoI CTCGAG 1 cut(s) 1432
XspI CTAG 3 cut(s) 1409, 1490, 1566
ZrmI AGTACT 2 cut(s) 914, 1430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.