Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0000522

Physical Location & Seq

Genomic Coordinates Reverse (-)

36832802 .. 36834862

Gene Structure 2061 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1290900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1710 bp

ATGGCTTATAATTCTAAGCTAATCTCATCCCTCAATCTTTTAGCTTCATTTCTCATCCTTCTCGTTCCACTCTTTGCCACTCTATCTTTAGCCGCGGATAACTCTTCAACCACTGTCTCTGCCGGAACCATTTGCAAATCCACCCCCGACCCTTCCTTTTGCAAATCTGTCCTCCCTCACAACCACAACCCCGAAAATGTCTACTTCTACGGCCGGTTCTCCGTCCGAAAGTCCTTATCCCAATCCCGAGAATTCTTAAAACTTATCGACAAGTATCTCCAAAGACGTTCTACTTTGTCACTTACCGCCGTCCGAGCTCTCGAGGACTGCCTGCTGCTTGCCGGCCTCAACATGGACTTTTTGCTCAACAGTTTTGAAACCGTCAATAAAACTAGCAAAACCCTTTCCACACTCAAAGCCAACGACGTCCAAACCCTCCTCAGCGCCATTCTCACCAACCAGCAAACATGTTTCGATGGCATTCAGTCCACCGCGTCGGCGTGGAGTCTGAAAAACGGCCTCTCCGCCCCGCTCAAAAACGACACTAAATTCTACAGCGTTTCACTAGCACTTTTTACCAAAGGGTGGGTTCCCAAGAAGAAGAAACAAGCCGCGTGGCGCCCGACTAGGATGCAGCGGGGCTTCAAGAACGGACGCTTGCCGCTGAAGATGTCGAGCAAAACGCGTGCCGTTTACGAGAGCGTGAGCAAGCGGAAGATGCTGCAGACGGAGACGGCGGACGATGAAGTGTTAGTGAGTGAGATCGTGACGGTGAGTCGGGACGGAACCGGAAACTTCTCCACCATCAATGACGCCATTGCGGCGGCTCCAAACAACTCTGCACCAACTGATGGGTACTTCTTGATCTATGTCACAGCCGGTGTTTATGAAGAGTACGTCTCGGTTGCTAAAAATAAGAGGTACTTAATGATGGTCGGAGACGGCATTAACCAGACGATTATCACCGGTAACCGGAGCGTTGTTGATGGATGGACAACTTTCAACTCTGCAACATTTGCTGTGGTTGCACCTGGATTTGTAGCGGTGAACATAACGTTCCGCAACACCGCCGGAGCAATCAAGCACCAAGCGGTTGCAGTCCGAAGCGGCGCGGATCTGTCAACCTTTTATAGTTGCAGCTTTGAAGCGTACCAAGACACACTATACACACATTCTCTTCGGCAATTCTACAGAGAATGTGACATATACGGCACCGTAGACTTCATATTTGGAAATGCTGCAGTGGTTTTCCAAAACTGCAACATTTATCCCCGCCTGCCGATGAGCGGTCAGTTCAACGCCATTACGGCTCAGGGTAGAACAGATCCGAACCAAAACACAGGCACTTCGATTCATAATTGCACCATTCGTGCCGCCGATGATTTAGCCTCTAGCAACAGCAGTACAAAGACTTATTTGGGCAGGCCATGGAAGGAGTATTCTAGGACAGTTTACATGCAGTCTTACATTGATAGCTTGGTGGAACCTGTTGGTTGGCGCGCTTGGGACGGAGATTTTGCGCTAAGCACATTGTATTACGCTGAGTACAACAACACTGGACCCGGATCGAACACTACGAACAGAGTTACGTGGCCCGGTTATCATGTGATTAATTCTACCGACGCGGCTAATTTCACGGTGACTAATTTCTTGCTGGGAGATGATTGGTTGCCTCAAACCGGAGTGCCTTACACCGGCGGATTAATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009620 GO:0009628 GO:0009719 GO:0009725 GO:0009741 GO:0010033 GO:0014070 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0033993 GO:0042221 GO:0043086 GO:0043207 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0050896 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

570

Amino Acids

62.77

Weight (kDa)

9.01

Isoelectric Point (pI)

33.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	43 - 191	2.3e-25	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	256 - 554	1.3e-139	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000522)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02810 AT2G47550 AT4G02330
fragaria_vesca	FvH4_2g25960 FvH4_2g25960 FvH4_2g25970 FvH4_5g35570 FvH4_5g35580 FvH4_5g35590
malus_domestica	MD03G1290900.v1.1 MD03G1291000.v1.1 MD08G1195600.v1.1 MD11G1307400.v1.1 MD11G1307500.v1.1
prunus_persica	Prupe.1G529400_v2.0.a1 Prupe.1G529500_v2.0.a1 Prupe.8G263900_v2.0.a1 Prupe.8G264000_v2.0.a1
pyrus_communis	pycom08g16800
rosa_chinensis	RchiOBHm_Chr6g0294321 RchiOBHm_Chr6g0294331 RchiOBHm_Chr7g0236951 RchiOBHm_Chr7g0236971 RchiOBHm_Chr7g0237041 RchiOBHm_Chr7g0237051
rosa_laevigata	RLG00000001034 RLG00000001035 RLG00000001039 RLG00000001043 RLG00000001045 RLG00000011880 RLG00000011881 RLG00000034181
rosa_multiflora	Rmu_co8086188.1_g000001 Rmu_co8458485.1_g000001 Rmu_sc0000272.1_g000007 Rmu_sc0000272.1_g000009 Rmu_sc0002131.1_g000030 Rmu_sc0002131.1_g000037 Rmu_sc0005369.1_g000005 Rmu_sc0005369.1_g000008 Rmu_sc0007991.1_g000005 Rmu_sc0007991.1_g000013 Rmu_sc0033563.1_g000001
rosa_roxburghii	Rroxscaffold_3G00224540 Rroxscaffold_3G00224600 Rroxscaffold_3G00224650 Rroxscaffold_3G00224660 Rroxscaffold_7G00173470 Rroxscaffold_7G00173480
rosa_rugosa	Rorug06G0243400 Rorug06G0243500 Rorug07G0301200 Rorug07G0301500 Rorug07G0301600
rosa_samantha	Rh6AG356000 Rh6AG356100 Rh6BG362600 Rh6BG362800 Rh6CG370000 Rh6CG370100 Rh6DG356500 Rh6DG356600 Rh7AG456200 Rh7AG456500 Rh7AG457500 Rh7AG457600 Rh7BG427500 Rh7BG427700 Rh7BG428400 Rh7BG428500 Rh7CG475100 Rh7CG475300 Rh7CG475700 Rh7CG475800 Rh7DG444100 Rh7DG444300 Rh7DG444700 Rh7DG444900
rosa_wichuraiana	Rw6G031020 Rw6G031030 Rw7G037870 Rw7G037880 Rw7G037920 Rw7G037940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	9
AatII	GACGTC	1 cut(s)	429
AccB1I	GGYRCC	2 cut(s)	618, 1211
AccB7I	CCANNNNNTGG	2 cut(s)	585, 851
AccBSI	CCGCTC	2 cut(s)	532, 1287
AccI	GTMKAC	2 cut(s)	201, 1218
AccII	CGCG	7 cut(s)	95, 494, 614, 685, 1112, 1500, 1625
AclI	AACGTT	1 cut(s)	1055
AclWI	GGATC	3 cut(s)	1122, 1319, 1573
AcoI	YGGCCR	1 cut(s)	211
AcsI	RAATTY	2 cut(s)	251, 548
AcuI	CTGAAG	1 cut(s)	686
AcyI	GRCGYC	3 cut(s)	426, 619, 813
AfaI	GTAC	6 cut(s)	857, 896, 923, 1151, 1405, 1547
AfiI	CCNNNNNNNGG	7 cut(s)	352, 585, 851, 972, 1286, 1679, 1694
AflIII	ACRYGT	2 cut(s)	467, 683
AgeI	ACCGGT	1 cut(s)	965
AgsI	TTSAA	6 cut(s)	108, 377, 646, 1003, 1145, 1297
AhdI	GACNNNNNGTC	1 cut(s)	774
AjnI	CCWGG	1 cut(s)	1030
AluBI	AGCT	5 cut(s)	19, 44, 317, 1140, 1476
AluI	AGCT	5 cut(s)	19, 44, 317, 1140, 1476
Alw21I	GWGCWC	1 cut(s)	319
Alw26I	GTCTC	4 cut(s)	121, 725, 904, 933
AlwI	GGATC	3 cut(s)	1122, 1319, 1573
Ama87I	CYCGRG	2 cut(s)	246, 320
AoxI	GGCC	5 cut(s)	211, 343, 517, 1424, 1592
ApeKI	GCWGC	5 cut(s)	334, 634, 721, 1137, 1238
ApoI	RAATTY	2 cut(s)	251, 548
ArsI	GACNNNNNNTTYG	4 cut(s)	1211, 1243, 1499, 1531
AseI	ATTAAT	2 cut(s)	1611, 1703
AsiGI	ACCGGT	1 cut(s)	965
AspLEI	GCGC	6 cut(s)	446, 621, 1112, 1500, 1502, 1522
AspS9I	GGNCC	2 cut(s)	1559, 1593
AsuC2I	CCSGG	2 cut(s)	1563, 1596
AsuHPI	GGTGA	5 cut(s)	445, 784, 955, 1057, 1651
AvaI	CYCGRG	2 cut(s)	246, 320
AvaII	GGWCC	1 cut(s)	1559
BanI	GGYRCC	2 cut(s)	618, 1211
BanII	GRGCYC	1 cut(s)	319
Bbv12I	GWGCWC	1 cut(s)	319
BbvCI	CCTCAGC	1 cut(s)	440
BbvI	GCAGC	5 cut(s)	321, 646, 708, 1149, 1225
BccI	CCATC	6 cut(s)	470, 812, 845, 925, 980, 984
BceAI	ACGGC	8 cut(s)	226, 293, 532, 674, 750, 958, 1225, 1323
BcgI	CGANNNNNNTGC	2 cut(s)	613, 647
BciT130I	CCWGG	1 cut(s)	1032
BcnI	CCSGG	2 cut(s)	1563, 1596
BcoDI	GTCTC	4 cut(s)	121, 725, 904, 933
BfaI	CTAG	5 cut(s)	393, 566, 627, 1392, 1443
BfmI	CTRYAG	4 cut(s)	553, 722, 1189, 1239
BfoI	RGCGCY	2 cut(s)	447, 622
BglI	GCCNNNNNGGC	2 cut(s)	821, 1307
BlpI	GCTNAGC	1 cut(s)	1523
Bme1390I	CCNGG	3 cut(s)	1032, 1563, 1596
Bme18I	GGWCC	1 cut(s)	1559
BmeRI	GACNNNNNGTC	1 cut(s)	774
BmeT110I	CYCGRG	2 cut(s)	246, 320
BmgT120I	GGNCC	2 cut(s)	1559, 1593
BmiI	GGNNCC	8 cut(s)	127, 591, 620, 787, 828, 1213, 1485, 1561
BmrFI	CCNGG	3 cut(s)	1032, 1563, 1596
BmsI	GCATC	2 cut(s)	621, 708
BplI	GAGNNNNNCTC	2 cut(s)	884, 916
Bpu10I	CCTNAGC	2 cut(s)	440, 1311
Bpu1102I	GCTNAGC	1 cut(s)	1523
BpuMI	CCSGG	2 cut(s)	1563, 1596
BsaAI	YACGTR	1 cut(s)	1590
BsaHI	GRCGYC	3 cut(s)	426, 619, 813
BsaJI	CCNNGG	2 cut(s)	93, 1427
BsaWI	WCCGGW	4 cut(s)	788, 965, 972, 1679
BsaXI	ACNNNNNCTCC	2 cut(s)	506, 536
Bsc4I	CCNNNNNNNGG	7 cut(s)	352, 585, 851, 972, 1286, 1679, 1694
Bse118I	RCCGGY	5 cut(s)	213, 341, 878, 965, 1694
Bse1I	ACTGG	1 cut(s)	1561
Bse3DI	GCAATG	1 cut(s)	816
BseBI	CCWGG	1 cut(s)	1032
BseDI	CCNNGG	2 cut(s)	93, 1427
BseGI	GGATG	4 cut(s)	26, 54, 636, 995
BseLI	CCNNNNNNNGG	7 cut(s)	352, 585, 851, 972, 1286, 1679, 1694
BseMI	GCAATG	1 cut(s)	816
BseMII	CTCAG	3 cut(s)	454, 1325, 1533
BseNI	ACTGG	1 cut(s)	1561
BsePI	GCGCGC	1 cut(s)	1498
BseRI	GAGGAG	1 cut(s)	428
BseX3I	CGGCCG	1 cut(s)	211
BseXI	GCAGC	5 cut(s)	321, 646, 708, 1149, 1225
BseYI	CCCAGC	1 cut(s)	1654
BsgI	GTGCAG	1 cut(s)	825
Bsh1236I	CGCG	7 cut(s)	95, 494, 614, 685, 1112, 1500, 1625
Bsh1285I	CGRYCG	1 cut(s)	214
BshFI	GGCC	5 cut(s)	213, 345, 519, 1426, 1594
BshNI	GGYRCC	2 cut(s)	618, 1211
BshTI	ACCGGT	1 cut(s)	965
BsiEI	CGRYCG	1 cut(s)	214
BsiHKAI	GWGCWC	1 cut(s)	319
BsiHKCI	CYCGRG	2 cut(s)	246, 320
BslFI	GGGAC	2 cut(s)	794, 1520
BslI	CCNNNNNNNGG	7 cut(s)	352, 585, 851, 972, 1286, 1679, 1694
BsmAI	GTCTC	4 cut(s)	121, 725, 904, 933
BsmBI	CGTCTC	3 cut(s)	725, 904, 933
BsmFI	GGGAC	2 cut(s)	794, 1520
BsmI	GAATGC	1 cut(s)	480
BsnI	GGCC	5 cut(s)	213, 345, 519, 1426, 1594
BsoBI	CYCGRG	2 cut(s)	246, 320
Bsp1286I	GDGCHC	1 cut(s)	319
Bsp143I	GATC	5 cut(s)	762, 864, 1114, 1324, 1565
Bsp1720I	GCTNAGC	1 cut(s)	1523
Bsp19I	CCATGG	1 cut(s)	1427
BspANI	GGCC	5 cut(s)	213, 345, 519, 1426, 1594
BspCNI	CTCAG	3 cut(s)	453, 1324, 1534
BspFNI	CGCG	7 cut(s)	95, 494, 614, 685, 1112, 1500, 1625
BspLI	GGNNCC	8 cut(s)	127, 591, 620, 787, 828, 1213, 1485, 1561
BspMAI	CTGCAG	2 cut(s)	726, 1243
BspPI	GGATC	3 cut(s)	1122, 1319, 1573
BspT107I	GGYRCC	2 cut(s)	618, 1211
BsrBI	CCGCTC	2 cut(s)	532, 1287
BsrDI	GCAATG	1 cut(s)	816
BsrFI	RCCGGY	5 cut(s)	213, 341, 878, 965, 1694
BsrI	ACTGG	1 cut(s)	1561
BssAI	RCCGGY	5 cut(s)	213, 341, 878, 965, 1694
BssECI	CCNNGG	2 cut(s)	93, 1427
BssHII	GCGCGC	1 cut(s)	1498
BssMI	GATC	5 cut(s)	762, 864, 1114, 1324, 1565
BssNI	GRCGYC	3 cut(s)	426, 619, 813
BssT1I	CCWWGG	1 cut(s)	1427
Bst2UI	CCWGG	1 cut(s)	1032
Bst4CI	ACNGT	7 cut(s)	115, 371, 382, 772, 1216, 1450, 1639
Bst6I	CTCTTC	3 cut(s)	109, 885, 1182
BstACI	GRCGYC	3 cut(s)	426, 619, 813
BstAPI	GCANNNNNTGC	1 cut(s)	1016
BstBAI	YACGTR	1 cut(s)	1590
BstC8I	GCNNGC	9 cut(s)	332, 339, 343, 659, 687, 710, 1277, 1424, 1500
BstDEI	CTNAG	5 cut(s)	15, 440, 1311, 1523, 1542
BstDSI	CCRYGG	2 cut(s)	93, 1427
BstEII	GGTNACC	1 cut(s)	968
BstF5I	GGATG	4 cut(s)	26, 54, 636, 995
BstFNI	CGCG	7 cut(s)	95, 494, 614, 685, 1112, 1500, 1625
BstH2I	RGCGCY	2 cut(s)	447, 622
BstHHI	GCGC	6 cut(s)	446, 621, 1112, 1500, 1502, 1522
BstKTI	GATC	5 cut(s)	765, 867, 1117, 1327, 1568
BstMAI	GTCTC	4 cut(s)	121, 725, 904, 933
BstMBI	GATC	5 cut(s)	762, 864, 1114, 1324, 1565
BstMCI	CGRYCG	1 cut(s)	214
BstMWI	GCNNNNNNNGC	6 cut(s)	314, 718, 821, 1016, 1025, 1307
BstNI	CCWGG	1 cut(s)	1032
BstNSI	RCATGY	2 cut(s)	471, 1459
BstPI	GGTNACC	1 cut(s)	968
BstSCI	CCNGG	3 cut(s)	1030, 1561, 1594
BstSFI	CTRYAG	4 cut(s)	553, 722, 1189, 1239
BstUI	CGCG	7 cut(s)	95, 494, 614, 685, 1112, 1500, 1625
BstV1I	GCAGC	5 cut(s)	321, 646, 708, 1149, 1225
BstX2I	RGATCY	2 cut(s)	1114, 1324
BstYI	RGATCY	2 cut(s)	1114, 1324
BstZI	CGGCCG	1 cut(s)	211
BsuRI	GGCC	5 cut(s)	213, 345, 519, 1426, 1594
BtgI	CCRYGG	2 cut(s)	93, 1427
BtsCI	GGATG	4 cut(s)	26, 54, 636, 995
BtsI	GCAGTG	1 cut(s)	1248
BtsIMutI	CAGTG	3 cut(s)	111, 1248, 1554
Cac8I	GCNNGC	9 cut(s)	332, 339, 343, 659, 687, 710, 1277, 1424, 1500
CfoI	GCGC	6 cut(s)	446, 621, 1112, 1500, 1502, 1522
Cfr10I	RCCGGY	5 cut(s)	213, 341, 878, 965, 1694
Cfr13I	GGNCC	2 cut(s)	1559, 1593
Cfr42I	CCGCGG	1 cut(s)	96
CseI	GACGC	4 cut(s)	483, 663, 821, 1631
Csp6I	GTAC	6 cut(s)	856, 895, 922, 1150, 1404, 1546
CspAI	ACCGGT	1 cut(s)	965
CspCI	CAANNNNNGTGG	2 cut(s)	596, 631
CviAII	CATG	5 cut(s)	352, 468, 1428, 1456, 1604
CviQI	GTAC	6 cut(s)	856, 895, 922, 1150, 1404, 1546
DdeI	CTNAG	5 cut(s)	15, 440, 1311, 1523, 1542
DinI	GGCGCC	1 cut(s)	620
DpnI	GATC	5 cut(s)	764, 866, 1116, 1326, 1567
DpnII	GATC	5 cut(s)	762, 864, 1114, 1324, 1565
DriI	GACNNNNNGTC	1 cut(s)	774
EaeI	YGGCCR	1 cut(s)	211
EagI	CGGCCG	1 cut(s)	211
Eam1104I	CTCTTC	3 cut(s)	109, 885, 1182
Eam1105I	GACNNNNNGTC	1 cut(s)	774
EarI	CTCTTC	3 cut(s)	109, 885, 1182
EciI	GGCGGA	2 cut(s)	514, 752
Ecl136II	GAGCTC	1 cut(s)	317
EclXI	CGGCCG	1 cut(s)	211
Eco130I	CCWWGG	1 cut(s)	1427
Eco24I	GRGCYC	1 cut(s)	319
Eco47I	GGWCC	1 cut(s)	1559
Eco52I	CGGCCG	1 cut(s)	211
Eco53kI	GAGCTC	1 cut(s)	317
Eco57I	CTGAAG	1 cut(s)	686
Eco88I	CYCGRG	2 cut(s)	246, 320
Eco91I	GGTNACC	1 cut(s)	968
EcoICRI	GAGCTC	1 cut(s)	317
EcoO65I	GGTNACC	1 cut(s)	968
EcoRI	GAATTC	1 cut(s)	251
EcoRII	CCWGG	1 cut(s)	1030
EcoT14I	CCWWGG	1 cut(s)	1427
EcoT38I	GRGCYC	1 cut(s)	319
EgeI	GGCGCC	1 cut(s)	620
EheI	GGCGCC	1 cut(s)	620
ErhI	CCWWGG	1 cut(s)	1427
Esp3I	CGTCTC	3 cut(s)	725, 904, 933
FaeI	CATG	5 cut(s)	355, 471, 1431, 1459, 1607
FalI	AAGNNNNNCTT	2 cut(s)	908, 940
FaqI	GGGAC	2 cut(s)	794, 1520
FatI	CATG	5 cut(s)	351, 467, 1427, 1455, 1603
FauI	CCCGC	3 cut(s)	537, 630, 1280
FblI	GTMKAC	2 cut(s)	201, 1218
FokI	GGATG	4 cut(s)	13, 41, 643, 1002
FriOI	GRGCYC	1 cut(s)	319
FspBI	CTAG	5 cut(s)	393, 566, 627, 1392, 1443
GlaI	GCGC	6 cut(s)	445, 620, 1111, 1499, 1501, 1521
GsaI	CCCAGC	1 cut(s)	1658
HaeII	RGCGCY	2 cut(s)	447, 622
HaeIII	GGCC	5 cut(s)	213, 345, 519, 1426, 1594
HgaI	GACGC	4 cut(s)	483, 663, 821, 1631
HhaI	GCGC	6 cut(s)	446, 621, 1112, 1500, 1502, 1522
Hin1I	GRCGYC	3 cut(s)	426, 619, 813
Hin1II	CATG	5 cut(s)	355, 471, 1431, 1459, 1607
Hin6I	GCGC	6 cut(s)	444, 619, 1110, 1498, 1500, 1520
HinP1I	GCGC	6 cut(s)	444, 619, 1110, 1498, 1500, 1520
HincII	GTYRAC	1 cut(s)	1122
HindII	GTYRAC	1 cut(s)	1122
HinfI	GANTC	3 cut(s)	505, 775, 1351
HphI	GGTGA	5 cut(s)	445, 784, 955, 1057, 1651
Hpy166II	GTNNAC	7 cut(s)	202, 489, 694, 1048, 1122, 1219, 1453
Hpy188I	TCNGA	6 cut(s)	227, 314, 510, 938, 1103, 1329
Hpy188III	TCNNGA	6 cut(s)	246, 320, 646, 766, 779, 862
Hpy8I	GTNNAC	7 cut(s)	202, 489, 694, 1048, 1122, 1219, 1453
Hpy99I	CGWCG	3 cut(s)	428, 499, 1625
HpyAV	CCTTC	3 cut(s)	68, 162, 1426
HpyCH4III	ACNGT	7 cut(s)	115, 371, 382, 772, 1216, 1450, 1639
HpyCH4IV	ACGT	5 cut(s)	286, 426, 897, 1055, 1589
HpyF10VI	GCNNNNNNNGC	6 cut(s)	314, 718, 821, 1016, 1025, 1307
HpyF3I	CTNAG	5 cut(s)	15, 440, 1311, 1523, 1542
HpySE526I	ACGT	5 cut(s)	286, 426, 897, 1055, 1589
Hsp92I	GRCGYC	3 cut(s)	426, 619, 813
Hsp92II	CATG	5 cut(s)	355, 471, 1431, 1459, 1607
HspAI	GCGC	6 cut(s)	444, 619, 1110, 1498, 1500, 1520
KasI	GGCGCC	1 cut(s)	618
KroI	GCCGGC	1 cut(s)	341
KroNI	GCCGGC	1 cut(s)	343
KspI	CCGCGG	1 cut(s)	96
Kzo9I	GATC	5 cut(s)	762, 864, 1114, 1324, 1565
LmnI	GCTCC	3 cut(s)	832, 975, 1073
Lsp1109I	GCAGC	5 cut(s)	321, 646, 708, 1149, 1225
LweI	GCATC	2 cut(s)	621, 708
MaeI	CTAG	5 cut(s)	393, 566, 627, 1392, 1443
MaeII	ACGT	5 cut(s)	286, 426, 897, 1055, 1589
MaeIII	GTNAC	7 cut(s)	297, 766, 871, 968, 1199, 1585, 1639
MalI	GATC	5 cut(s)	764, 866, 1116, 1326, 1567
MbiI	CCGCTC	2 cut(s)	532, 1287
MboI	GATC	5 cut(s)	762, 864, 1114, 1324, 1565
MboII	GAAGA	7 cut(s)	96, 610, 613, 679, 727, 902, 1169
MflI	RGATCY	2 cut(s)	1114, 1324
MhlI	GDGCHC	1 cut(s)	319
MluCI	AATT	9 cut(s)	10, 251, 548, 1184, 1357, 1612, 1630, 1645, 1704
MluI	ACGCGT	1 cut(s)	683
Mly113I	GGCGCC	1 cut(s)	619
MlyI	GAGTC	2 cut(s)	514, 784
MmeI	TCCRAC	1 cut(s)	916
MroNI	GCCGGC	1 cut(s)	341
MseI	TTAA	5 cut(s)	257, 926, 948, 1611, 1703
MspA1I	CMGCKG	3 cut(s)	95, 637, 664
MspR9I	CCNGG	3 cut(s)	1032, 1563, 1596
Mva1269I	GAATGC	1 cut(s)	480
MvaI	CCWGG	1 cut(s)	1032
MvnI	CGCG	7 cut(s)	95, 494, 614, 685, 1112, 1500, 1625
MwoI	GCNNNNNNNGC	6 cut(s)	314, 718, 821, 1016, 1025, 1307
NaeI	GCCGGC	1 cut(s)	343
NarI	GGCGCC	1 cut(s)	619
NciI	CCSGG	2 cut(s)	1563, 1596
NcoI	CCATGG	1 cut(s)	1427
NdeII	GATC	5 cut(s)	762, 864, 1114, 1324, 1565
NgoMIV	GCCGGC	1 cut(s)	341
NlaIII	CATG	5 cut(s)	355, 471, 1431, 1459, 1607
NlaIV	GGNNCC	8 cut(s)	127, 591, 620, 787, 828, 1213, 1485, 1561
NmuCI	GTSAC	5 cut(s)	297, 766, 871, 1199, 1639
NspI	RCATGY	2 cut(s)	471, 1459
PaeR7I	CTCGAG	1 cut(s)	320
PauI	GCGCGC	1 cut(s)	1498
PciI	ACATGT	1 cut(s)	467
PcsI	WCGNNNNNNNCGW	1 cut(s)	1574
PctI	GAATGC	1 cut(s)	480
PdiI	GCCGGC	1 cut(s)	343
PfeI	GAWTC	1 cut(s)	1351
PflMI	CCANNNNNTGG	2 cut(s)	585, 851
PinAI	ACCGGT	1 cut(s)	965
PleI	GAGTC	2 cut(s)	513, 783
PluTI	GGCGCC	1 cut(s)	622
PpsI	GAGTC	2 cut(s)	513, 783
Ppu21I	YACGTR	1 cut(s)	1590
PscI	ACATGT	1 cut(s)	467
PshBI	ATTAAT	2 cut(s)	1611, 1703
PsiI	TTATAA	1 cut(s)	9
Psp124BI	GAGCTC	1 cut(s)	319
Psp1406I	AACGTT	1 cut(s)	1055
Psp6I	CCWGG	1 cut(s)	1030
PspEI	GGTNACC	1 cut(s)	968
PspFI	CCCAGC	1 cut(s)	1654
PspGI	CCWGG	1 cut(s)	1030
PspN4I	GGNNCC	8 cut(s)	127, 591, 620, 787, 828, 1213, 1485, 1561
PspPI	GGNCC	2 cut(s)	1559, 1593
PstI	CTGCAG	2 cut(s)	726, 1243
PsuI	RGATCY	2 cut(s)	1114, 1324
PteI	GCGCGC	1 cut(s)	1498
RsaI	GTAC	6 cut(s)	857, 896, 923, 1151, 1405, 1547
RsaNI	GTAC	6 cut(s)	856, 895, 922, 1150, 1404, 1546
SacI	GAGCTC	1 cut(s)	319
SacII	CCGCGG	1 cut(s)	96
SaqAI	TTAA	5 cut(s)	257, 926, 948, 1611, 1703
Sau3AI	GATC	5 cut(s)	762, 864, 1114, 1324, 1565
Sau96I	GGNCC	2 cut(s)	1559, 1593
SchI	GAGTC	2 cut(s)	514, 784
ScrFI	CCNGG	3 cut(s)	1032, 1563, 1596
SduI	GDGCHC	1 cut(s)	319
SfaNI	GCATC	2 cut(s)	621, 708
SfcI	CTRYAG	4 cut(s)	553, 722, 1189, 1239
SfoI	GGCGCC	1 cut(s)	620
Sfr274I	CTCGAG	1 cut(s)	320
Sfr303I	CCGCGG	1 cut(s)	96
SgrAI	CRCCGGYG	1 cut(s)	1694
SgrBI	CCGCGG	1 cut(s)	96
SinI	GGWCC	1 cut(s)	1559
SlaI	CTCGAG	1 cut(s)	320
SmlI	CTYRAG	1 cut(s)	320
SmoI	CTYRAG	1 cut(s)	320
Sse9I	AATT	9 cut(s)	10, 251, 548, 1184, 1357, 1612, 1630, 1645, 1704
SspDI	GGCGCC	1 cut(s)	618
SspMI	CTAG	5 cut(s)	393, 566, 627, 1392, 1443
SstI	GAGCTC	1 cut(s)	319
StyD4I	CCNGG	3 cut(s)	1030, 1561, 1594
StyI	CCWWGG	1 cut(s)	1427
TaaI	ACNGT	7 cut(s)	115, 371, 382, 772, 1216, 1450, 1639
TaiI	ACGT	5 cut(s)	289, 429, 900, 1058, 1592
TaqI	TCGA	6 cut(s)	267, 321, 474, 674, 1349, 1568
TasI	AATT	9 cut(s)	10, 251, 548, 1184, 1357, 1612, 1630, 1645, 1704
TatI	WGTACW	2 cut(s)	1403, 1545
TauI	GCSGC	8 cut(s)	95, 614, 664, 824, 827, 1110, 1376, 1628
TfiI	GAWTC	1 cut(s)	1351
Tru1I	TTAA	5 cut(s)	257, 926, 948, 1611, 1703
Tru9I	TTAA	5 cut(s)	257, 926, 948, 1611, 1703
TscAI	CASTG	3 cut(s)	118, 1248, 1561
TseFI	GTSAC	5 cut(s)	297, 766, 871, 1199, 1639
TseI	GCWGC	5 cut(s)	334, 634, 721, 1137, 1238
Tsp45I	GTSAC	5 cut(s)	297, 766, 871, 1199, 1639
TspDTI	ATGAA	5 cut(s)	36, 759, 903, 1213, 1343
TspGWI	ACGGA	5 cut(s)	211, 666, 743, 798, 1524
TspRI	CASTG	3 cut(s)	118, 1248, 1561
Van91I	CCANNNNNTGG	2 cut(s)	585, 851
VpaK11BI	GGWCC	1 cut(s)	1559
VspI	ATTAAT	2 cut(s)	1611, 1703
XapI	RAATTY	2 cut(s)	251, 548
XceI	RCATGY	2 cut(s)	471, 1459
XhoI	CTCGAG	1 cut(s)	320
XmiI	GTMKAC	2 cut(s)	201, 1218
XspI	CTAG	5 cut(s)	393, 566, 627, 1392, 1443
ZraI	GACGTC	1 cut(s)	427

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.