Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0000522

Physical Location & Seq

Genomic Coordinates Reverse (-)

57406954 .. 57408784

Gene Structure 1831 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G037870.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1701 bp

ATGGCTTCTTCAAAGCTCTTTCCTCTTGTAACCTTTTCATCAATATTATTTCTCACCATCTTCTCTTTGCTTATTTCTCCATCTCTAGCAGTAGATGTTCCTCTAAGCACTCCTCTTCCATCAGAAACCATATGCAACTCCACTCCACACCCTTCTTACTGCAAATCTGTCCTCCCTCACAAAAGCGCCAATGTCTATGACTTGGGGCGCTTTTCAGTCCAACAGTCACTCCTTCAATCCAAAAGCCTTTTGGATTTGCTTGAAAAATATCTCCAAGGTCGGCGTTCCAGTCTAACCCTACCCGCAATCCAAGCCCTTGAGGATTGTCACTTCCTTGCTTCGTTAAACATTGATTTTTTGTCGCATTCCCTCCGAACCATTGGCCAAACTAGTAATGTTCTTCCAAGCTCAGATGCTGATGATGTCCAAACCTTGCTCAGTGCCATTTTAACCAACCAGCAAACTTGTGCTGATGGCCTCAATTCTTTGACCACTGTCCACAACGATCTCATAATCCCACTCTCTAATAGTACAAATTTGTACAGTGTCTCTTTAGCTCTGTTTACAAAGGGCTGGGTGCCAAAGGGTCGCAGAAATAATGTGATACCAAATCAGTCCAACAAGCATCTCAATTTCAGAAAGGGACGATTGTCACTGAAAATGTCAAGCAAAGCACGTGCAGTTTACGATTCCGTTATAAATCAAAGAAGACACCTTCTTCAGGCAGGAGATAATGAAGTTGTGGTGAAGGATATTGTGGTTGTGAGCCAGGATGGAAGTGGAAACTTCCCAACCATCAACGATGCTATTACTGCTGCTCCAAATAACTCTGTTGCTAGTGATGGGTACTTCTTGATATATATCACTGCTGGTGTTTATCAAGAGTACGTTTCGATTTCATCAAAAAAGAAATACTTGCTTATGATTGGAGATGGTATCAACCAGACAATCATCACGGGAAACCACAGCGTTGGAGATGGGTGGACCACTTTCAACTCTCCTACTTTTGCTGTGGCGGCTCAAGGATTTGTAGCAGTAAACATTACTTTTCAAAACACGGCTGGACCAAGCAAGGGGCAAGCAGTGGCAGTTAGAAATGATGGCGATTTGTCGGTTTTCTATAGCTGCAGCTTTGAAGCCTACCAAGACACGTTGTACACACATTCTATGAGACAATTCTACAGAGAATGTGATATCTATGGAACTGTTGACTTCATTTTCGGCAATGCTGCTGTTGTGTTCCAAAACTGCAACATATACCCTCGTCGTCCCCTCCAAGGCCAATCAAATACCATCACAGCTCAAGGGCGGACCGATGTGAATCAGAATACCGGGATATCAATCCAGAATTGCACAGTTACAGCCGCAGCCGATCTGGCTTCGAGTAGTTTCAGTGTCAAGAATTATCTGGGGAGGCCATGGAAGGAATACTCGAGGACAATTTTCATGCAGGGTTTCATCGATAGTTTTATAGAACCTGCTGGTTGGCTTGCGTGGAATGGAGATTTTGCACTGAGCACATTGTATTATGCTGAGTACAATAATAGAGGACCTGGATCAAATACTAGCAACAGAGTCACGTGGCCTGGTTACCATGTGATCGGTGCAACTGATGCTGCCAACTTTACAGTCTCCAACTTCATATTGGGGGATAATTGGCTGCCTCAGACAGAAGTGCCTTACACTGGTGGCTTAATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

566

Amino Acids

61.95

Weight (kDa)

6.79

Isoelectric Point (pI)

41.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	42 - 187	9.9e-25	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	253 - 551	1.2e-138	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000522)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02810 AT2G47550 AT4G02330
fragaria_vesca	FvH4_2g25960 FvH4_2g25960 FvH4_2g25970 FvH4_5g35570 FvH4_5g35580 FvH4_5g35590
malus_domestica	MD03G1290900.v1.1 MD03G1291000.v1.1 MD08G1195600.v1.1 MD11G1307400.v1.1 MD11G1307500.v1.1
prunus_persica	Prupe.1G529400_v2.0.a1 Prupe.1G529500_v2.0.a1 Prupe.8G263900_v2.0.a1 Prupe.8G264000_v2.0.a1
pyrus_communis	pycom08g16800
rosa_chinensis	RchiOBHm_Chr6g0294321 RchiOBHm_Chr6g0294331 RchiOBHm_Chr7g0236951 RchiOBHm_Chr7g0236971 RchiOBHm_Chr7g0237041 RchiOBHm_Chr7g0237051
rosa_laevigata	RLG00000001034 RLG00000001035 RLG00000001039 RLG00000001043 RLG00000001045 RLG00000011880 RLG00000011881 RLG00000034181
rosa_multiflora	Rmu_co8086188.1_g000001 Rmu_co8458485.1_g000001 Rmu_sc0000272.1_g000007 Rmu_sc0000272.1_g000009 Rmu_sc0002131.1_g000030 Rmu_sc0002131.1_g000037 Rmu_sc0005369.1_g000005 Rmu_sc0005369.1_g000008 Rmu_sc0007991.1_g000005 Rmu_sc0007991.1_g000013 Rmu_sc0033563.1_g000001
rosa_roxburghii	Rroxscaffold_3G00224540 Rroxscaffold_3G00224600 Rroxscaffold_3G00224650 Rroxscaffold_3G00224660 Rroxscaffold_7G00173470 Rroxscaffold_7G00173480
rosa_rugosa	Rorug06G0243400 Rorug06G0243500 Rorug07G0301200 Rorug07G0301500 Rorug07G0301600
rosa_samantha	Rh6AG356000 Rh6AG356100 Rh6BG362600 Rh6BG362800 Rh6CG370000 Rh6CG370100 Rh6DG356500 Rh6DG356600 Rh7AG456200 Rh7AG456500 Rh7AG457500 Rh7AG457600 Rh7BG427500 Rh7BG427700 Rh7BG428400 Rh7BG428500 Rh7CG475100 Rh7CG475300 Rh7CG475700 Rh7CG475800 Rh7DG444100 Rh7DG444300 Rh7DG444700 Rh7DG444900
rosa_wichuraiana	Rw6G031020 Rw6G031030 Rw7G037870 Rw7G037880 Rw7G037920 Rw7G037940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	698
Acc36I	ACCTGC	1 cut(s)	1486
AccB1I	GGYRCC	1 cut(s)	577
AciI	CCGC	4 cut(s)	303, 1016, 1309, 1365
AclWI	GGATC	1 cut(s)	1564
AcoI	YGGCCR	1 cut(s)	382
AcsI	RAATTY	1 cut(s)	535
AcuI	CTGAAG	1 cut(s)	704
AcvI	CACGTG	2 cut(s)	677, 1581
AfaI	GTAC	6 cut(s)	532, 542, 848, 887, 1157, 1538
AfiI	CCNNNNNNNGG	4 cut(s)	721, 1073, 1277, 1685
AflIII	ACRYGT	1 cut(s)	1149
AgsI	TTSAA	6 cut(s)	12, 236, 263, 994, 1052, 1136
AhlI	ACTAGT	1 cut(s)	389
AjnI	CCWGG	3 cut(s)	768, 1552, 1585
AluBI	AGCT	6 cut(s)	16, 408, 557, 1125, 1131, 1301
AluI	AGCT	6 cut(s)	16, 408, 557, 1125, 1131, 1301
Alw21I	GWGCWC	1 cut(s)	1520
Alw26I	GTCTC	3 cut(s)	553, 1165, 1636
AlwI	GGATC	1 cut(s)	1564
AlwNI	CAGNNNCTG	1 cut(s)	416
Ama87I	CYCGRG	1 cut(s)	1432
AoxI	GGCC	5 cut(s)	382, 475, 1279, 1415, 1583
ApeKI	GCWGC	7 cut(s)	815, 1125, 1128, 1229, 1367, 1616, 1660
ApoI	RAATTY	1 cut(s)	535
ArsI	GACNNNNNNTTYG	2 cut(s)	1202, 1234
AspLEI	GCGC	2 cut(s)	188, 210
AspS9I	GGNCC	4 cut(s)	984, 1064, 1311, 1550
AsuC2I	CCSGG	1 cut(s)	1333
AsuHPI	GGTGA	2 cut(s)	46, 757
AvaI	CYCGRG	1 cut(s)	1432
AvaII	GGWCC	4 cut(s)	984, 1064, 1311, 1550
BalI	TGGCCA	1 cut(s)	384
BanI	GGYRCC	1 cut(s)	577
BbrPI	CACGTG	2 cut(s)	677, 1581
BbsI	GAAGAC	1 cut(s)	715
Bbv12I	GWGCWC	1 cut(s)	1520
BbvI	GCAGC	7 cut(s)	802, 1112, 1140, 1216, 1379, 1603, 1647
BceAI	ACGGC	1 cut(s)	1074
BciT130I	CCWGG	3 cut(s)	770, 1554, 1587
BcnI	CCSGG	1 cut(s)	1333
BcoDI	GTCTC	3 cut(s)	553, 1165, 1636
BcuI	ACTAGT	1 cut(s)	389
BfaI	CTAG	4 cut(s)	86, 390, 837, 1566
BfmI	CTRYAG	3 cut(s)	1120, 1126, 1180
BfoI	RGCGCY	2 cut(s)	189, 211
BfuAI	ACCTGC	1 cut(s)	1486
BglI	GCCNNNNNGGC	1 cut(s)	1376
BisI	GCNGC	9 cut(s)	816, 1017, 1126, 1129, 1230, 1365, 1368, 1617, 1661
BlsI	GCNGC	9 cut(s)	817, 1018, 1127, 1130, 1231, 1366, 1369, 1618, 1662
Bme1390I	CCNGG	4 cut(s)	770, 1333, 1554, 1587
Bme18I	GGWCC	4 cut(s)	984, 1064, 1311, 1550
BmeT110I	CYCGRG	1 cut(s)	1432
BmgT120I	GGNCC	4 cut(s)	984, 1064, 1311, 1550
BmiI	GGNNCC	1 cut(s)	579
BmrFI	CCNGG	4 cut(s)	770, 1333, 1554, 1587
BmsI	GCATC	4 cut(s)	403, 634, 793, 1603
BoxI	GACNNNNGTC	2 cut(s)	494, 649
BpiI	GAAGAC	1 cut(s)	715
BpuEI	CTTGAG	3 cut(s)	338, 1005, 1287
BpuMI	CCSGG	1 cut(s)	1333
Bsa29I	ATCGAT	1 cut(s)	1461
BsaAI	YACGTR	2 cut(s)	677, 1581
BsaBI	GATNNNNATC	3 cut(s)	860, 1320, 1340
BsaJI	CCNNGG	3 cut(s)	274, 1276, 1418
BsaXI	ACNNNNNCTCC	4 cut(s)	213, 243, 802, 832
Bsc4I	CCNNNNNNNGG	4 cut(s)	721, 1073, 1277, 1685
Bse1I	ACTGG	2 cut(s)	288, 1690
Bse3DI	GCAATG	1 cut(s)	1231
Bse8I	GATNNNNATC	3 cut(s)	860, 1320, 1340
BseBI	CCWGG	3 cut(s)	770, 1554, 1587
BseCI	ATCGAT	1 cut(s)	1461
BseDI	CCNNGG	3 cut(s)	274, 1276, 1418
BseGI	GGATG	1 cut(s)	778
BseJI	GATNNNNATC	3 cut(s)	860, 1320, 1340
BseLI	CCNNNNNNNGG	4 cut(s)	721, 1073, 1277, 1685
BseMI	GCAATG	1 cut(s)	1231
BseMII	CTCAG	5 cut(s)	423, 451, 1505, 1524, 1679
BseNI	ACTGG	2 cut(s)	288, 1690
BseRI	GAGGAG	1 cut(s)	102
BseXI	GCAGC	7 cut(s)	802, 1112, 1140, 1216, 1379, 1603, 1647
BseYI	CCCAGC	1 cut(s)	573
BsgI	GTGCAG	1 cut(s)	699
BshFI	GGCC	5 cut(s)	384, 477, 1281, 1417, 1585
BshNI	GGYRCC	1 cut(s)	577
BshVI	ATCGAT	1 cut(s)	1461
BsiHKAI	GWGCWC	1 cut(s)	1520
BsiHKCI	CYCGRG	1 cut(s)	1432
BsiSI	CCGG	1 cut(s)	1332
BslFI	GGGAC	2 cut(s)	657, 1254
BslI	CCNNNNNNNGG	4 cut(s)	721, 1073, 1277, 1685
BsmAI	GTCTC	3 cut(s)	553, 1165, 1636
BsmFI	GGGAC	2 cut(s)	657, 1254
BsmI	GAATGC	1 cut(s)	364
BsnI	GGCC	5 cut(s)	384, 477, 1281, 1417, 1585
BsoBI	CYCGRG	1 cut(s)	1432
Bsp1286I	GDGCHC	1 cut(s)	1520
Bsp1407I	TGTACA	2 cut(s)	540, 1155
Bsp143I	GATC	4 cut(s)	505, 1372, 1556, 1599
Bsp19I	CCATGG	1 cut(s)	1418
BspACI	CCGC	4 cut(s)	303, 1016, 1309, 1365
BspANI	GGCC	5 cut(s)	384, 477, 1281, 1417, 1585
BspCNI	CTCAG	5 cut(s)	422, 450, 1506, 1525, 1678
BspDI	ATCGAT	1 cut(s)	1461
BspLI	GGNNCC	1 cut(s)	579
BspMAI	CTGCAG	1 cut(s)	1130
BspMI	ACCTGC	1 cut(s)	1486
BspPI	GGATC	1 cut(s)	1564
BspT107I	GGYRCC	1 cut(s)	577
BsrDI	GCAATG	1 cut(s)	1231
BsrGI	TGTACA	2 cut(s)	540, 1155
BsrI	ACTGG	2 cut(s)	288, 1690
BssECI	CCNNGG	3 cut(s)	274, 1276, 1418
BssMI	GATC	4 cut(s)	505, 1372, 1556, 1599
BssT1I	CCWWGG	3 cut(s)	274, 1276, 1418
Bst2UI	CCWGG	3 cut(s)	770, 1554, 1587
Bst4CI	ACNGT	6 cut(s)	225, 496, 545, 1207, 1357, 1630
Bst6I	CTCTTC	1 cut(s)	120
BstAPI	GCANNNNNTGC	1 cut(s)	1613
BstAUI	TGTACA	2 cut(s)	540, 1155
BstBAI	YACGTR	2 cut(s)	677, 1581
BstC8I	GCNNGC	2 cut(s)	1080, 1491
BstDEI	CTNAG	6 cut(s)	104, 409, 437, 1514, 1533, 1665
BstDSI	CCRYGG	1 cut(s)	1418
BstEII	GGTNACC	1 cut(s)	1589
BstENI	CCTNNNNNAGG	1 cut(s)	719
BstF5I	GGATG	1 cut(s)	778
BstH2I	RGCGCY	2 cut(s)	189, 211
BstHHI	GCGC	2 cut(s)	188, 210
BstKTI	GATC	4 cut(s)	508, 1375, 1559, 1602
BstMAI	GTCTC	3 cut(s)	553, 1165, 1636
BstMBI	GATC	4 cut(s)	505, 1372, 1556, 1599
BstMWI	GCNNNNNNNGC	5 cut(s)	311, 812, 1016, 1376, 1613
BstNI	CCWGG	3 cut(s)	770, 1554, 1587
BstPAI	GACNNNNGTC	2 cut(s)	494, 649
BstPI	GGTNACC	1 cut(s)	1589
BstSCI	CCNGG	4 cut(s)	768, 1331, 1552, 1585
BstSFI	CTRYAG	3 cut(s)	1120, 1126, 1180
BstV1I	GCAGC	7 cut(s)	802, 1112, 1140, 1216, 1379, 1603, 1647
BstV2I	GAAGAC	1 cut(s)	715
BstXI	CCANNNNNNTGG	1 cut(s)	971
Bsu15I	ATCGAT	1 cut(s)	1461
BsuRI	GGCC	5 cut(s)	384, 477, 1281, 1417, 1585
BsuTUI	ATCGAT	1 cut(s)	1461
BtgI	CCRYGG	1 cut(s)	1418
BtsCI	GGATG	1 cut(s)	778
BtsI	GCAGTG	2 cut(s)	864, 1089
BtsIMutI	CAGTG	9 cut(s)	445, 492, 550, 653, 864, 1089, 1399, 1511, 1683
BveI	ACCTGC	1 cut(s)	1486
Cac8I	GCNNGC	2 cut(s)	1080, 1491
CaiI	CAGNNNCTG	1 cut(s)	416
CfoI	GCGC	2 cut(s)	188, 210
Cfr13I	GGNCC	4 cut(s)	984, 1064, 1311, 1550
ClaI	ATCGAT	1 cut(s)	1461
CpoI	CGGWCCG	1 cut(s)	1311
Csp6I	GTAC	6 cut(s)	531, 541, 847, 886, 1156, 1537
CspI	CGGWCCG	1 cut(s)	1311
CviAII	CATG	3 cut(s)	1419, 1447, 1595
CviQI	GTAC	6 cut(s)	531, 541, 847, 886, 1156, 1537
DdeI	CTNAG	6 cut(s)	104, 409, 437, 1514, 1533, 1665
DpnI	GATC	4 cut(s)	507, 1374, 1558, 1601
DpnII	GATC	4 cut(s)	505, 1372, 1556, 1599
EaeI	YGGCCR	1 cut(s)	382
Eam1104I	CTCTTC	1 cut(s)	120
EarI	CTCTTC	1 cut(s)	120
EciI	GGCGGA	1 cut(s)	1324
Eco130I	CCWWGG	3 cut(s)	274, 1276, 1418
Eco32I	GATATC	2 cut(s)	1195, 1338
Eco47I	GGWCC	4 cut(s)	984, 1064, 1311, 1550
Eco57I	CTGAAG	1 cut(s)	704
Eco72I	CACGTG	2 cut(s)	677, 1581
Eco88I	CYCGRG	1 cut(s)	1432
Eco91I	GGTNACC	1 cut(s)	1589
EcoNI	CCTNNNNNAGG	1 cut(s)	719
EcoO109I	RGGNCCY	1 cut(s)	1550
EcoO65I	GGTNACC	1 cut(s)	1589
EcoRII	CCWGG	3 cut(s)	768, 1552, 1585
EcoRV	GATATC	2 cut(s)	1195, 1338
EcoT14I	CCWWGG	3 cut(s)	274, 1276, 1418
ErhI	CCWWGG	3 cut(s)	274, 1276, 1418
FaeI	CATG	3 cut(s)	1422, 1450, 1598
FalI	AAGNNNNNCTT	2 cut(s)	899, 931
FaqI	GGGAC	2 cut(s)	657, 1254
FatI	CATG	3 cut(s)	1418, 1446, 1594
FauI	CCCGC	1 cut(s)	310
FauNDI	CATATG	1 cut(s)	131
Fnu4HI	GCNGC	9 cut(s)	816, 1017, 1126, 1129, 1230, 1365, 1368, 1617, 1661
FokI	GGATG	1 cut(s)	785
Fsp4HI	GCNGC	9 cut(s)	816, 1017, 1126, 1129, 1230, 1365, 1368, 1617, 1661
FspBI	CTAG	4 cut(s)	86, 390, 837, 1566
GlaI	GCGC	2 cut(s)	187, 209
GluI	GCNGC	9 cut(s)	816, 1017, 1126, 1129, 1230, 1365, 1368, 1617, 1661
GsaI	CCCAGC	1 cut(s)	577
HaeII	RGCGCY	2 cut(s)	189, 211
HaeIII	GGCC	5 cut(s)	384, 477, 1281, 1417, 1585
HapII	CCGG	1 cut(s)	1332
HhaI	GCGC	2 cut(s)	188, 210
Hin1II	CATG	3 cut(s)	1422, 1450, 1598
Hin6I	GCGC	2 cut(s)	186, 208
HinP1I	GCGC	2 cut(s)	186, 208
HincII	GTYRAC	1 cut(s)	1210
HindII	GTYRAC	1 cut(s)	1210
HinfI	GANTC	3 cut(s)	689, 1321, 1575
HpaII	CCGG	1 cut(s)	1332
HphI	GGTGA	2 cut(s)	46, 757
Hpy166II	GTNNAC	7 cut(s)	499, 564, 685, 984, 1039, 1158, 1210
Hpy188I	TCNGA	6 cut(s)	124, 374, 412, 638, 1326, 1668
Hpy188III	TCNNGA	4 cut(s)	853, 881, 1345, 1399
Hpy8I	GTNNAC	7 cut(s)	499, 564, 685, 984, 1039, 1158, 1210
Hpy99I	CGWCG	1 cut(s)	1269
HpyAV	CCTTC	5 cut(s)	162, 242, 725, 742, 1417
HpyCH4III	ACNGT	6 cut(s)	225, 496, 545, 1207, 1357, 1630
HpyCH4IV	ACGT	4 cut(s)	676, 888, 1151, 1580
HpyCH4V	TGCA	9 cut(s)	135, 162, 680, 1128, 1251, 1353, 1450, 1511, 1607
HpyF10VI	GCNNNNNNNGC	5 cut(s)	311, 812, 1016, 1376, 1613
HpyF3I	CTNAG	6 cut(s)	104, 409, 437, 1514, 1533, 1665
HpySE526I	ACGT	4 cut(s)	676, 888, 1151, 1580
Hsp92II	CATG	3 cut(s)	1422, 1450, 1598
HspAI	GCGC	2 cut(s)	186, 208
Kzo9I	GATC	4 cut(s)	505, 1372, 1556, 1599
LmnI	GCTCC	1 cut(s)	823
Lsp1109I	GCAGC	7 cut(s)	802, 1112, 1140, 1216, 1379, 1603, 1647
LweI	GCATC	4 cut(s)	403, 634, 793, 1603
MaeI	CTAG	4 cut(s)	86, 390, 837, 1566
MaeII	ACGT	4 cut(s)	676, 888, 1151, 1580
MaeIII	GTNAC	7 cut(s)	28, 225, 326, 651, 1357, 1576, 1589
MalI	GATC	4 cut(s)	507, 1374, 1558, 1601
MboI	GATC	4 cut(s)	505, 1372, 1556, 1599
MboII	GAAGA	5 cut(s)	52, 107, 392, 710, 720
MhlI	GDGCHC	1 cut(s)	1520
MlsI	TGGCCA	1 cut(s)	384
MluCI	AATT	9 cut(s)	481, 535, 631, 1175, 1348, 1402, 1440, 1654, 1695
MluNI	TGGCCA	1 cut(s)	384
MlyI	GAGTC	1 cut(s)	1584
MmeI	TCCRAC	4 cut(s)	244, 642, 952, 1659
Mox20I	TGGCCA	1 cut(s)	384
MscI	TGGCCA	1 cut(s)	384
MseI	TTAA	3 cut(s)	344, 449, 1694
Msp20I	TGGCCA	1 cut(s)	384
MspI	CCGG	1 cut(s)	1332
MspR9I	CCNGG	4 cut(s)	770, 1333, 1554, 1587
Mva1269I	GAATGC	1 cut(s)	364
MvaI	CCWGG	3 cut(s)	770, 1554, 1587
MwoI	GCNNNNNNNGC	5 cut(s)	311, 812, 1016, 1376, 1613
NciI	CCSGG	1 cut(s)	1333
NcoI	CCATGG	1 cut(s)	1418
NdeI	CATATG	1 cut(s)	131
NdeII	GATC	4 cut(s)	505, 1372, 1556, 1599
NlaIII	CATG	3 cut(s)	1422, 1450, 1598
NlaIV	GGNNCC	1 cut(s)	579
NmuCI	GTSAC	4 cut(s)	225, 326, 651, 1576
PaeR7I	CTCGAG	1 cut(s)	1432
PctI	GAATGC	1 cut(s)	364
PfeI	GAWTC	2 cut(s)	689, 1321
PkrI	GCNGC	9 cut(s)	817, 1018, 1127, 1130, 1231, 1366, 1369, 1618, 1662
PleI	GAGTC	1 cut(s)	1583
PmaCI	CACGTG	2 cut(s)	677, 1581
PmlI	CACGTG	2 cut(s)	677, 1581
PpsI	GAGTC	1 cut(s)	1583
Ppu21I	YACGTR	2 cut(s)	677, 1581
PpuMI	RGGWCCY	1 cut(s)	1550
PshAI	GACNNNNGTC	2 cut(s)	494, 649
PsiI	TTATAA	1 cut(s)	698
Psp5II	RGGWCCY	1 cut(s)	1550
Psp6I	CCWGG	3 cut(s)	768, 1552, 1585
PspCI	CACGTG	2 cut(s)	677, 1581
PspEI	GGTNACC	1 cut(s)	1589
PspFI	CCCAGC	1 cut(s)	573
PspGI	CCWGG	3 cut(s)	768, 1552, 1585
PspN4I	GGNNCC	1 cut(s)	579
PspPI	GGNCC	4 cut(s)	984, 1064, 1311, 1550
PspPPI	RGGWCCY	1 cut(s)	1550
PspXI	VCTCGAGB	1 cut(s)	1432
PstI	CTGCAG	1 cut(s)	1130
PstNI	CAGNNNCTG	1 cut(s)	416
RsaI	GTAC	6 cut(s)	532, 542, 848, 887, 1157, 1538
RsaNI	GTAC	6 cut(s)	531, 541, 847, 886, 1156, 1537
Rsr2I	CGGWCCG	1 cut(s)	1311
RsrII	CGGWCCG	1 cut(s)	1311
SaqAI	TTAA	3 cut(s)	344, 449, 1694
SatI	GCNGC	9 cut(s)	816, 1017, 1126, 1129, 1230, 1365, 1368, 1617, 1661
Sau3AI	GATC	4 cut(s)	505, 1372, 1556, 1599
Sau96I	GGNCC	4 cut(s)	984, 1064, 1311, 1550
SchI	GAGTC	1 cut(s)	1584
ScrFI	CCNGG	4 cut(s)	770, 1333, 1554, 1587
SduI	GDGCHC	1 cut(s)	1520
SfaNI	GCATC	4 cut(s)	403, 634, 793, 1603
SfcI	CTRYAG	3 cut(s)	1120, 1126, 1180
Sfr274I	CTCGAG	1 cut(s)	1432
SinI	GGWCC	4 cut(s)	984, 1064, 1311, 1550
SlaI	CTCGAG	1 cut(s)	1432
SmlI	CTYRAG	4 cut(s)	317, 1020, 1302, 1432
SmoI	CTYRAG	4 cut(s)	317, 1020, 1302, 1432
SpeI	ACTAGT	1 cut(s)	389
Sse9I	AATT	9 cut(s)	481, 535, 631, 1175, 1348, 1402, 1440, 1654, 1695
SsiI	CCGC	4 cut(s)	303, 1016, 1309, 1365
SspI	AATATT	1 cut(s)	45
SspMI	CTAG	4 cut(s)	86, 390, 837, 1566
StyD4I	CCNGG	4 cut(s)	768, 1331, 1552, 1585
StyI	CCWWGG	3 cut(s)	274, 1276, 1418
TaaI	ACNGT	6 cut(s)	225, 496, 545, 1207, 1357, 1630
TaiI	ACGT	4 cut(s)	679, 891, 1154, 1583
TaqI	TCGA	4 cut(s)	893, 1382, 1433, 1461
TaqII	GACCGA	1 cut(s)	1328
TasI	AATT	9 cut(s)	481, 535, 631, 1175, 1348, 1402, 1440, 1654, 1695
TatI	WGTACW	4 cut(s)	530, 540, 1155, 1536
TauI	GCSGC	2 cut(s)	1019, 1367
TfiI	GAWTC	2 cut(s)	689, 1321
Tru1I	TTAA	3 cut(s)	344, 449, 1694
Tru9I	TTAA	3 cut(s)	344, 449, 1694
TscAI	CASTG	9 cut(s)	445, 499, 550, 660, 871, 1089, 1399, 1518, 1690
TseFI	GTSAC	4 cut(s)	225, 326, 651, 1576
TseI	GCWGC	7 cut(s)	815, 1125, 1128, 1229, 1367, 1616, 1660
Tsp45I	GTSAC	4 cut(s)	225, 326, 651, 1576
TspDTI	ATGAA	7 cut(s)	27, 750, 888, 1204, 1435, 1447, 1630
TspGWI	ACGGA	1 cut(s)	682
TspRI	CASTG	9 cut(s)	445, 499, 550, 660, 871, 1089, 1399, 1518, 1690
VpaK11BI	GGWCC	4 cut(s)	984, 1064, 1311, 1550
XagI	CCTNNNNNAGG	1 cut(s)	719
XapI	RAATTY	1 cut(s)	535
XcmI	CCANNNNNNNNNTGG	3 cut(s)	247, 776, 1642
XhoI	CTCGAG	1 cut(s)	1432
XspI	CTAG	4 cut(s)	86, 390, 837, 1566

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.