Pectinesterase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 887 |
| Acc65I | GGTACC | 1 cut(s) | 854 |
| AccB1I | GGYRCC | 2 cut(s) | 835, 854 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 564 |
| AccI | GTMKAC | 1 cut(s) | 993 |
| AccII | CGCG | 4 cut(s) | 171, 214, 422, 696 |
| AclI | AACGTT | 1 cut(s) | 377 |
| AclWI | GGATC | 2 cut(s) | 113, 1328 |
| AcoI | YGGCCR | 1 cut(s) | 178 |
| AcsI | RAATTY | 5 cut(s) | 125, 218, 802, 1353, 1654 |
| AcyI | GRCGYC | 2 cut(s) | 836, 884 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 480, 564, 967, 981 |
| AflIII | ACRYGT | 1 cut(s) | 443 |
| AgeI | ACCGGT | 3 cut(s) | 974, 1495, 1565 |
| AgsI | TTSAA | 7 cut(s) | 47, 84, 1012, 1131, 1306, 1493, 1578 |
| AjnI | CCWGG | 1 cut(s) | 1095 |
| AluBI | AGCT | 8 cut(s) | 287, 529, 665, 824, 1106, 1157, 1250, 1319 |
| AluI | AGCT | 8 cut(s) | 287, 529, 665, 824, 1106, 1157, 1250, 1319 |
| Alw21I | GWGCWC | 1 cut(s) | 289 |
| Alw26I | GTCTC | 2 cut(s) | 341, 544 |
| AlwI | GGATC | 2 cut(s) | 113, 1328 |
| AoxI | GGCC | 4 cut(s) | 178, 606, 1098, 1601 |
| ApaI | GGGCCC | 1 cut(s) | 610 |
| ApeKI | GCWGC | 1 cut(s) | 1247 |
| ApoI | RAATTY | 5 cut(s) | 125, 218, 802, 1353, 1654 |
| AseI | ATTAAT | 1 cut(s) | 1620 |
| AsiGI | ACCGGT | 3 cut(s) | 974, 1495, 1565 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1301, 1640 |
| Asp718I | GGTACC | 1 cut(s) | 854 |
| AspLEI | GCGC | 2 cut(s) | 698, 838 |
| AspS9I | GGNCC | 5 cut(s) | 606, 607, 1002, 1082, 1602 |
| AsuC2I | CCSGG | 1 cut(s) | 1605 |
| AsuHPI | GGTGA | 3 cut(s) | 547, 793, 959 |
| AsuII | TTCGAA | 1 cut(s) | 1152 |
| AvaII | GGWCC | 2 cut(s) | 1002, 1082 |
| BaeGI | GKGCMC | 1 cut(s) | 610 |
| BanI | GGYRCC | 2 cut(s) | 835, 854 |
| BanII | GRGCYC | 2 cut(s) | 289, 610 |
| Bbv12I | GWGCWC | 1 cut(s) | 289 |
| BbvI | GCAGC | 1 cut(s) | 1259 |
| BccI | CCATC | 4 cut(s) | 446, 618, 1664, 1672 |
| BceAI | ACGGC | 6 cut(s) | 193, 263, 285, 683, 1037, 1085 |
| BciT130I | CCWGG | 1 cut(s) | 1097 |
| BcnI | CCSGG | 1 cut(s) | 1605 |
| BcoDI | GTCTC | 2 cut(s) | 341, 544 |
| BfaI | CTAG | 1 cut(s) | 308 |
| BfmI | CTRYAG | 3 cut(s) | 1138, 1198, 1584 |
| BfoI | RGCGCY | 1 cut(s) | 839 |
| BglII | AGATCT | 1 cut(s) | 1123 |
| BisI | GCNGC | 4 cut(s) | 181, 423, 468, 1248 |
| BlsI | GCNGC | 4 cut(s) | 182, 424, 469, 1249 |
| BmcAI | AGTACT | 1 cut(s) | 1582 |
| Bme1390I | CCNGG | 2 cut(s) | 1097, 1605 |
| Bme18I | GGWCC | 2 cut(s) | 1002, 1082 |
| BmgT120I | GGNCC | 5 cut(s) | 606, 607, 1002, 1082, 1602 |
| BmiI | GGNNCC | 5 cut(s) | 570, 608, 837, 856, 1570 |
| BmrFI | CCNGG | 2 cut(s) | 1097, 1605 |
| BmsI | GCATC | 2 cut(s) | 1281, 1621 |
| Bpu14I | TTCGAA | 1 cut(s) | 1152 |
| BpuEI | CTTGAG | 2 cut(s) | 141, 1305 |
| BpuMI | CCSGG | 1 cut(s) | 1605 |
| BsaAI | YACGTR | 2 cut(s) | 880, 1454 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1338 |
| BsaHI | GRCGYC | 2 cut(s) | 836, 884 |
| BsaJI | CCNNGG | 2 cut(s) | 420, 1436 |
| BsaWI | WCCGGW | 5 cut(s) | 974, 981, 1495, 1565, 1571 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 480, 564, 967, 981 |
| Bse118I | RCCGGY | 5 cut(s) | 860, 974, 1381, 1495, 1565 |
| Bse1I | ACTGG | 2 cut(s) | 594, 1300 |
| Bse3DI | GCAATG | 3 cut(s) | 57, 503, 1249 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1338 |
| BseBI | CCWGG | 1 cut(s) | 1097 |
| BseDI | CCNNGG | 2 cut(s) | 420, 1436 |
| BseGI | GGATG | 4 cut(s) | 368, 610, 1636, 1683 |
| BseJI | GATNNNNATC | 1 cut(s) | 1338 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 480, 564, 967, 981 |
| BseMI | GCAATG | 3 cut(s) | 57, 503, 1249 |
| BseMII | CTCAG | 1 cut(s) | 1542 |
| BseNI | ACTGG | 2 cut(s) | 594, 1300 |
| BseRI | GAGGAG | 1 cut(s) | 1126 |
| BseSI | GKGCMC | 1 cut(s) | 610 |
| BseX3I | CGGCCG | 1 cut(s) | 178 |
| BseXI | GCAGC | 1 cut(s) | 1259 |
| BsgI | GTGCAG | 1 cut(s) | 1140 |
| Bsh1236I | CGCG | 4 cut(s) | 171, 214, 422, 696 |
| Bsh1285I | CGRYCG | 1 cut(s) | 181 |
| BshFI | GGCC | 4 cut(s) | 180, 608, 1100, 1603 |
| BshNI | GGYRCC | 2 cut(s) | 835, 854 |
| BshTI | ACCGGT | 3 cut(s) | 974, 1495, 1565 |
| BsiEI | CGRYCG | 1 cut(s) | 181 |
| BsiHKAI | GWGCWC | 1 cut(s) | 289 |
| BsiSI | CCGG | 8 cut(s) | 861, 975, 982, 1382, 1496, 1566, 1572, 1605 |
| BsiWI | CGTACG | 2 cut(s) | 962, 1454 |
| BslFI | GGGAC | 1 cut(s) | 1443 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 480, 564, 967, 981 |
| BsmAI | GTCTC | 2 cut(s) | 341, 544 |
| BsmBI | CGTCTC | 1 cut(s) | 341 |
| BsmFI | GGGAC | 1 cut(s) | 1443 |
| BsnI | GGCC | 4 cut(s) | 180, 608, 1100, 1603 |
| Bsp119I | TTCGAA | 1 cut(s) | 1152 |
| Bsp120I | GGGCCC | 1 cut(s) | 606 |
| Bsp1286I | GDGCHC | 2 cut(s) | 289, 610 |
| Bsp143I | GATC | 4 cut(s) | 118, 1123, 1333, 1588 |
| Bsp19I | CCATGG | 1 cut(s) | 1436 |
| Bsp68I | TCGCGA | 1 cut(s) | 171 |
| BspANI | GGCC | 4 cut(s) | 180, 608, 1100, 1603 |
| BspCNI | CTCAG | 1 cut(s) | 1543 |
| BspFNI | CGCG | 4 cut(s) | 171, 214, 422, 696 |
| BspLI | GGNNCC | 5 cut(s) | 570, 608, 837, 856, 1570 |
| BspPI | GGATC | 2 cut(s) | 113, 1328 |
| BspT104I | TTCGAA | 1 cut(s) | 1152 |
| BspT107I | GGYRCC | 2 cut(s) | 835, 854 |
| BsrDI | GCAATG | 3 cut(s) | 57, 503, 1249 |
| BsrFI | RCCGGY | 5 cut(s) | 860, 974, 1381, 1495, 1565 |
| BsrI | ACTGG | 2 cut(s) | 594, 1300 |
| BssAI | RCCGGY | 5 cut(s) | 860, 974, 1381, 1495, 1565 |
| BssECI | CCNNGG | 2 cut(s) | 420, 1436 |
| BssMI | GATC | 4 cut(s) | 118, 1123, 1333, 1588 |
| BssNI | GRCGYC | 2 cut(s) | 836, 884 |
| BssT1I | CCWWGG | 1 cut(s) | 1436 |
| Bst2UI | CCWGG | 1 cut(s) | 1097 |
| Bst6I | CTCTTC | 7 cut(s) | 23, 38, 48, 59, 78, 94, 481 |
| BstACI | GRCGYC | 2 cut(s) | 836, 884 |
| BstBAI | YACGTR | 2 cut(s) | 880, 1454 |
| BstBI | TTCGAA | 1 cut(s) | 1152 |
| BstC8I | GCNNGC | 2 cut(s) | 313, 546 |
| BstDEI | CTNAG | 2 cut(s) | 1187, 1551 |
| BstDSI | CCRYGG | 2 cut(s) | 420, 1436 |
| BstEII | GGTNACC | 2 cut(s) | 970, 977 |
| BstF5I | GGATG | 4 cut(s) | 368, 610, 1636, 1683 |
| BstFNI | CGCG | 4 cut(s) | 171, 214, 422, 696 |
| BstH2I | RGCGCY | 1 cut(s) | 839 |
| BstHHI | GCGC | 2 cut(s) | 698, 838 |
| BstKTI | GATC | 4 cut(s) | 121, 1126, 1336, 1591 |
| BstMAI | GTCTC | 2 cut(s) | 341, 544 |
| BstMBI | GATC | 4 cut(s) | 118, 1123, 1333, 1588 |
| BstMCI | CGRYCG | 1 cut(s) | 181 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 168, 177, 284, 1025, 1316 |
| BstNI | CCWGG | 1 cut(s) | 1097 |
| BstNSI | RCATGY | 1 cut(s) | 447 |
| BstPI | GGTNACC | 2 cut(s) | 970, 977 |
| BstSCI | CCNGG | 2 cut(s) | 1095, 1603 |
| BstSFI | CTRYAG | 3 cut(s) | 1138, 1198, 1584 |
| BstSLI | GKGCMC | 1 cut(s) | 610 |
| BstUI | CGCG | 4 cut(s) | 171, 214, 422, 696 |
| BstV1I | GCAGC | 1 cut(s) | 1259 |
| BstX2I | RGATCY | 1 cut(s) | 1123 |
| BstYI | RGATCY | 1 cut(s) | 1123 |
| BstZI | CGGCCG | 1 cut(s) | 178 |
| BsuRI | GGCC | 4 cut(s) | 180, 608, 1100, 1603 |
| BtgI | CCRYGG | 2 cut(s) | 420, 1436 |
| BtgZI | GCGATG | 1 cut(s) | 1020 |
| BtsCI | GGATG | 4 cut(s) | 368, 610, 1636, 1683 |
| BtsI | GCAGTG | 1 cut(s) | 1123 |
| BtsIMutI | CAGTG | 2 cut(s) | 144, 1123 |
| BtuMI | TCGCGA | 1 cut(s) | 171 |
| Cac8I | GCNNGC | 2 cut(s) | 313, 546 |
| CfoI | GCGC | 2 cut(s) | 698, 838 |
| Cfr10I | RCCGGY | 5 cut(s) | 860, 974, 1381, 1495, 1565 |
| Cfr13I | GGNCC | 5 cut(s) | 606, 607, 1002, 1082, 1602 |
| Cfr42I | CCGCGG | 1 cut(s) | 423 |
| CspAI | ACCGGT | 3 cut(s) | 974, 1495, 1565 |
| CviAII | CATG | 5 cut(s) | 444, 480, 1060, 1437, 1613 |
| DdeI | CTNAG | 2 cut(s) | 1187, 1551 |
| DinI | GGCGCC | 1 cut(s) | 837 |
| DpnI | GATC | 4 cut(s) | 120, 1125, 1335, 1590 |
| DpnII | GATC | 4 cut(s) | 118, 1123, 1333, 1588 |
| EaeI | YGGCCR | 1 cut(s) | 178 |
| EagI | CGGCCG | 1 cut(s) | 178 |
| Eam1104I | CTCTTC | 7 cut(s) | 23, 38, 48, 59, 78, 94, 481 |
| EarI | CTCTTC | 7 cut(s) | 23, 38, 48, 59, 78, 94, 481 |
| EciI | GGCGGA | 2 cut(s) | 265, 1006 |
| Ecl136II | GAGCTC | 1 cut(s) | 287 |
| EclXI | CGGCCG | 1 cut(s) | 178 |
| Eco130I | CCWWGG | 1 cut(s) | 1436 |
| Eco24I | GRGCYC | 2 cut(s) | 289, 610 |
| Eco32I | GATATC | 1 cut(s) | 932 |
| Eco47I | GGWCC | 2 cut(s) | 1002, 1082 |
| Eco52I | CGGCCG | 1 cut(s) | 178 |
| Eco53kI | GAGCTC | 1 cut(s) | 287 |
| Eco91I | GGTNACC | 2 cut(s) | 970, 977 |
| EcoICRI | GAGCTC | 1 cut(s) | 287 |
| EcoO65I | GGTNACC | 2 cut(s) | 970, 977 |
| EcoRII | CCWGG | 1 cut(s) | 1095 |
| EcoRV | GATATC | 1 cut(s) | 932 |
| EcoT14I | CCWWGG | 1 cut(s) | 1436 |
| EcoT38I | GRGCYC | 2 cut(s) | 289, 610 |
| EgeI | GGCGCC | 1 cut(s) | 837 |
| EheI | GGCGCC | 1 cut(s) | 837 |
| ErhI | CCWWGG | 1 cut(s) | 1436 |
| Esp3I | CGTCTC | 1 cut(s) | 341 |
| FaeI | CATG | 5 cut(s) | 447, 483, 1063, 1440, 1616 |
| FaqI | GGGAC | 1 cut(s) | 1443 |
| FatI | CATG | 5 cut(s) | 443, 479, 1059, 1436, 1612 |
| FauNDI | CATATG | 1 cut(s) | 101 |
| FblI | GTMKAC | 1 cut(s) | 993 |
| Fnu4HI | GCNGC | 4 cut(s) | 181, 423, 468, 1248 |
| FokI | GGATG | 4 cut(s) | 355, 597, 1643, 1690 |
| FriOI | GRGCYC | 2 cut(s) | 289, 610 |
| Fsp4HI | GCNGC | 4 cut(s) | 181, 423, 468, 1248 |
| FspBI | CTAG | 1 cut(s) | 308 |
| GlaI | GCGC | 2 cut(s) | 697, 837 |
| GluI | GCNGC | 4 cut(s) | 181, 423, 468, 1248 |
| HaeII | RGCGCY | 1 cut(s) | 839 |
| HaeIII | GGCC | 4 cut(s) | 180, 608, 1100, 1603 |
| HapII | CCGG | 8 cut(s) | 861, 975, 982, 1382, 1496, 1566, 1572, 1605 |
| HhaI | GCGC | 2 cut(s) | 698, 838 |
| Hin1I | GRCGYC | 2 cut(s) | 836, 884 |
| Hin1II | CATG | 5 cut(s) | 447, 483, 1063, 1440, 1616 |
| Hin6I | GCGC | 2 cut(s) | 696, 836 |
| HinP1I | GCGC | 2 cut(s) | 696, 836 |
| HincII | GTYRAC | 1 cut(s) | 994 |
| HindII | GTYRAC | 1 cut(s) | 994 |
| HindIII | AAGCTT | 1 cut(s) | 1155 |
| HinfI | GANTC | 4 cut(s) | 456, 707, 1339, 1360 |
| HpaII | CCGG | 8 cut(s) | 861, 975, 982, 1382, 1496, 1566, 1572, 1605 |
| HphI | GGTGA | 3 cut(s) | 547, 793, 959 |
| Hpy166II | GTNNAC | 7 cut(s) | 703, 751, 784, 994, 1057, 1694, 1712 |
| Hpy188I | TCNGA | 3 cut(s) | 1070, 1338, 1686 |
| Hpy188III | TCNNGA | 6 cut(s) | 170, 346, 449, 808, 1163, 1651 |
| Hpy8I | GTNNAC | 7 cut(s) | 703, 751, 784, 994, 1057, 1694, 1712 |
| Hpy99I | CGWCG | 5 cut(s) | 167, 401, 740, 995, 998 |
| HpyAV | CCTTC | 2 cut(s) | 90, 1435 |
| HpyCH4IV | ACGT | 9 cut(s) | 165, 377, 747, 879, 884, 906, 1421, 1453, 1538 |
| HpyCH4V | TGCA | 8 cut(s) | 105, 132, 260, 1121, 1146, 1247, 1294, 1468 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 168, 177, 284, 1025, 1316 |
| HpyF3I | CTNAG | 2 cut(s) | 1187, 1551 |
| HpySE526I | ACGT | 9 cut(s) | 165, 377, 747, 879, 884, 906, 1421, 1453, 1538 |
| Hsp92I | GRCGYC | 2 cut(s) | 836, 884 |
| Hsp92II | CATG | 5 cut(s) | 447, 483, 1063, 1440, 1616 |
| HspAI | GCGC | 2 cut(s) | 696, 836 |
| KasI | GGCGCC | 1 cut(s) | 835 |
| KpnI | GGTACC | 1 cut(s) | 858 |
| KspI | CCGCGG | 1 cut(s) | 423 |
| Kzo9I | GATC | 4 cut(s) | 118, 1123, 1333, 1588 |
| LmnI | GCTCC | 3 cut(s) | 284, 662, 1113 |
| Lsp1109I | GCAGC | 1 cut(s) | 1259 |
| LweI | GCATC | 2 cut(s) | 1281, 1621 |
| MaeI | CTAG | 1 cut(s) | 308 |
| MaeII | ACGT | 9 cut(s) | 165, 377, 747, 879, 884, 906, 1421, 1453, 1538 |
| MalI | GATC | 4 cut(s) | 120, 1125, 1335, 1590 |
| MboI | GATC | 4 cut(s) | 118, 1123, 1333, 1588 |
| MfeI | CAATTG | 1 cut(s) | 1366 |
| MflI | RGATCY | 1 cut(s) | 1123 |
| MhlI | GDGCHC | 2 cut(s) | 289, 610 |
| MluCI | AATT | 9 cut(s) | 125, 218, 802, 829, 1353, 1366, 1621, 1639, 1654 |
| Mly113I | GGCGCC | 1 cut(s) | 836 |
| MlyI | GAGTC | 1 cut(s) | 716 |
| MmeI | TCCRAC | 4 cut(s) | 262, 980, 1484, 1655 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1301, 1640 |
| MseI | TTAA | 6 cut(s) | 674, 713, 828, 1377, 1416, 1620 |
| MspA1I | CMGCKG | 3 cut(s) | 422, 1079, 1250 |
| MspI | CCGG | 8 cut(s) | 861, 975, 982, 1382, 1496, 1566, 1572, 1605 |
| MspR9I | CCNGG | 2 cut(s) | 1097, 1605 |
| MunI | CAATTG | 1 cut(s) | 1366 |
| MvaI | CCWGG | 1 cut(s) | 1097 |
| MvnI | CGCG | 4 cut(s) | 171, 214, 422, 696 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 168, 177, 284, 1025, 1316 |
| NarI | GGCGCC | 1 cut(s) | 836 |
| NciI | CCSGG | 1 cut(s) | 1605 |
| NcoI | CCATGG | 1 cut(s) | 1436 |
| NdeI | CATATG | 1 cut(s) | 101 |
| NdeII | GATC | 4 cut(s) | 118, 1123, 1333, 1588 |
| NlaIII | CATG | 5 cut(s) | 447, 483, 1063, 1440, 1616 |
| NlaIV | GGNNCC | 5 cut(s) | 570, 608, 837, 856, 1570 |
| NmuCI | GTSAC | 4 cut(s) | 629, 880, 947, 1208 |
| NruI | TCGCGA | 1 cut(s) | 171 |
| NspI | RCATGY | 1 cut(s) | 447 |
| NspV | TTCGAA | 1 cut(s) | 1152 |
| PciI | ACATGT | 1 cut(s) | 443 |
| PcsI | WCGNNNNNNNCGW | 5 cut(s) | 735, 744, 876, 1355, 1418 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1301, 1640 |
| PfeI | GAWTC | 3 cut(s) | 456, 1339, 1360 |
| Pfl23II | CGTACG | 2 cut(s) | 962, 1454 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 564 |
| PinAI | ACCGGT | 3 cut(s) | 974, 1495, 1565 |
| PkrI | GCNGC | 4 cut(s) | 182, 424, 469, 1249 |
| PleI | GAGTC | 1 cut(s) | 715 |
| PluTI | GGCGCC | 1 cut(s) | 839 |
| PpsI | GAGTC | 1 cut(s) | 715 |
| Ppu21I | YACGTR | 2 cut(s) | 880, 1454 |
| PscI | ACATGT | 1 cut(s) | 443 |
| PshBI | ATTAAT | 1 cut(s) | 1620 |
| Psp124BI | GAGCTC | 1 cut(s) | 289 |
| Psp1406I | AACGTT | 1 cut(s) | 377 |
| Psp6I | CCWGG | 1 cut(s) | 1095 |
| PspEI | GGTNACC | 2 cut(s) | 970, 977 |
| PspGI | CCWGG | 1 cut(s) | 1095 |
| PspLI | CGTACG | 2 cut(s) | 962, 1454 |
| PspN4I | GGNNCC | 5 cut(s) | 570, 608, 837, 856, 1570 |
| PspOMI | GGGCCC | 1 cut(s) | 606 |
| PspPI | GGNCC | 5 cut(s) | 606, 607, 1002, 1082, 1602 |
| PsuI | RGATCY | 1 cut(s) | 1123 |
| PvuII | CAGCTG | 1 cut(s) | 1250 |
| RruI | TCGCGA | 1 cut(s) | 171 |
| SacI | GAGCTC | 1 cut(s) | 289 |
| SacII | CCGCGG | 1 cut(s) | 423 |
| SalI | GTCGAC | 1 cut(s) | 992 |
| SaqAI | TTAA | 6 cut(s) | 674, 713, 828, 1377, 1416, 1620 |
| SatI | GCNGC | 4 cut(s) | 181, 423, 468, 1248 |
| Sau3AI | GATC | 4 cut(s) | 118, 1123, 1333, 1588 |
| Sau96I | GGNCC | 5 cut(s) | 606, 607, 1002, 1082, 1602 |
| ScaI | AGTACT | 1 cut(s) | 1582 |
| SchI | GAGTC | 1 cut(s) | 716 |
| ScrFI | CCNGG | 2 cut(s) | 1097, 1605 |
| SduI | GDGCHC | 2 cut(s) | 289, 610 |
| SfaNI | GCATC | 2 cut(s) | 1281, 1621 |
| SfcI | CTRYAG | 3 cut(s) | 1138, 1198, 1584 |
| SfoI | GGCGCC | 1 cut(s) | 837 |
| Sfr303I | CCGCGG | 1 cut(s) | 423 |
| SfuI | TTCGAA | 1 cut(s) | 1152 |
| SgrBI | CCGCGG | 1 cut(s) | 423 |
| SgrDI | CGTCGACG | 1 cut(s) | 992 |
| SinI | GGWCC | 2 cut(s) | 1002, 1082 |
| SmlI | CTYRAG | 2 cut(s) | 156, 1320 |
| SmoI | CTYRAG | 2 cut(s) | 156, 1320 |
| Sse9I | AATT | 9 cut(s) | 125, 218, 802, 829, 1353, 1366, 1621, 1639, 1654 |
| SspDI | GGCGCC | 1 cut(s) | 835 |
| SspMI | CTAG | 1 cut(s) | 308 |
| SstI | GAGCTC | 1 cut(s) | 289 |
| StyD4I | CCNGG | 2 cut(s) | 1095, 1603 |
| StyI | CCWWGG | 1 cut(s) | 1436 |
| TaiI | ACGT | 9 cut(s) | 168, 380, 750, 882, 887, 909, 1424, 1456, 1541 |
| TaqI | TCGA | 8 cut(s) | 345, 993, 1152, 1227, 1358, 1400, 1591, 1650 |
| TasI | AATT | 9 cut(s) | 125, 218, 802, 829, 1353, 1366, 1621, 1639, 1654 |
| TatI | WGTACW | 2 cut(s) | 1542, 1580 |
| TauI | GCSGC | 3 cut(s) | 183, 425, 470 |
| TfiI | GAWTC | 3 cut(s) | 456, 1339, 1360 |
| Tru1I | TTAA | 6 cut(s) | 674, 713, 828, 1377, 1416, 1620 |
| Tru9I | TTAA | 6 cut(s) | 674, 713, 828, 1377, 1416, 1620 |
| TscAI | CASTG | 2 cut(s) | 144, 1123 |
| TseFI | GTSAC | 4 cut(s) | 629, 880, 947, 1208 |
| TseI | GCWGC | 1 cut(s) | 1247 |
| Tsp45I | GTSAC | 4 cut(s) | 629, 880, 947, 1208 |
| TspDTI | ATGAA | 4 cut(s) | 46, 81, 468, 1222 |
| TspGWI | ACGGA | 9 cut(s) | 635, 644, 675, 698, 807, 833, 966, 1533, 1643 |
| TspRI | CASTG | 2 cut(s) | 144, 1123 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 564 |
| VpaK11BI | GGWCC | 2 cut(s) | 1002, 1082 |
| VspI | ATTAAT | 1 cut(s) | 1620 |
| XapI | RAATTY | 5 cut(s) | 125, 218, 802, 1353, 1654 |
| XceI | RCATGY | 1 cut(s) | 447 |
| XmiI | GTMKAC | 1 cut(s) | 993 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1301, 1640 |
| XspI | CTAG | 1 cut(s) | 308 |
| ZraI | GACGTC | 1 cut(s) | 885 |
| ZrmI | AGTACT | 1 cut(s) | 1582 |