Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0000522

Physical Location & Seq

Genomic Coordinates Forward (+)

13498799 .. 13500873

Gene Structure 2075 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000011880

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1638 bp

ATGGGTTCACAACCATTCTCTGTGTTCAAACACTCGACTCTCATTCTTCATCTTATTCCCATCCTTTTTGCCTCTCGATCTTTAGCTGCAGAGGCAGGTTGTGAAACTGATCCAGTATCCAAAGAAATCATTTGCGAATTCTCCCCTAACCCTTCATATTGCAAGTCCATGCTACCCAACCAAACAATCAATGTGTATGACTACGGCCGTTACGCGGTCCGGAAATCATTATTACAATCCCATAAATTACTTGACTTGGTGAACAAGTATCTCCAACACAACTCGACTCTCTCCAAAACCGCAATCCTCGCCCTTGAAGATTGCAAGCTTCTTGCAGAACTCAACGTAGAGTTTCTTGCAAGCTCCCTACAAACCCTAAACGACACTAACCGAACACTTCCCAGCTCGAAAGCCGAGGACGTCCATACCTGGCTCAGTGCTGTCCTAACAAACCAGCAAACATGCTTGGATGGCGTACAAGCAACTTCTTCTTCATCATCTTGGAGGAGTATGAAAAATGGACTTTTGCTCTCCAACAACACGAACCTTTATAGTGTTTCTCTAGCTCTGTTCACGAAGGGATGGGTGCCTGAAAATGTGGAAGACGAGGAGAAGGAGGAGGAGGGGGAGGTGATCACATGGAGGAAACGGCTGCTTCAGAATTTTGGTGACGTGCTGGTGAGGGAGACTGTCACTGTGAGTCAGGACGGAAGTGGGAACTTCACCACCATAACAGACGCTGTTGCCGCAGCTCCGGTTAACACTAAAGGTTTAAATGGTTACTTCTTGATTTATATCAAGGCAGGAGTTTATGAAGAGTATGTAAACATTGCAAATACGAAAAACTATCTGATGATGATTGGTGAGGGCATCAACCAGACTGTCATAACAGGCAATCGGAGTGTTGTAGATGGCTGGACAACTTTCAATTCAGCAACATTTGTGGTAATTGCACCATGGTTTGTAGCAGTGAACATAACCTTCCGCAACACCGCCGGAGCAATCAAACACCAAGCCGTCGCCGTCCGAAACGGTGGAGATTTGTCCGCATTCTATAATTGCAGCTTCGAAGGGTACCAAGACACCTTATACACACATTCCCAAAGACAATTCTACAGAGACTGCAACGTCTACGGAACAGTGGACTTCATATTTGGAAACGCAGCGGCTGTTTTCCAAAAATGTAACATATACCCCCGGCTTCCGATGCGTGGGCAGTTCAACGCCATTACGGCTCAGGGTCGAGCAGACCCGAATCAAAACACCGGCATTATGATCCACAACTGTGTCATCAGAGCTTCCGATGACTTGGCTTCGGATAAGGGAACTACCAGGACTTATCTAGGGAGGCCATGGAAGGAGTATTCAAGGACGGTGTACATGAAATCCTTCATGGATGGTTGCATAGATAGGGAAGGTTGGAGTAAATGGACTGGAGATTTTGCTTTAAGCACATTGTATTACGCCGAATATAAATGTCAGGGCCCTGGATCGAACACTAGTAGAAGAGTTACATGGCCAGGTTACCATATATTGAAAAAAGCTAAGCAAGCTAAAAAGTTTACAGTGTCCAAGTTCTTGCTTGGGAAGAAGTGGCTGCCTCAAACTGGAGTGCCATTTTCCGGTGGGTTGATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

546

Amino Acids

60.78

Weight (kDa)

8.68

Isoelectric Point (pI)

33.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	44 - 190	1.7e-20	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	231 - 529	5.1e-136	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000522)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02810 AT2G47550 AT4G02330
fragaria_vesca	FvH4_2g25960 FvH4_2g25960 FvH4_2g25970 FvH4_5g35570 FvH4_5g35580 FvH4_5g35590
malus_domestica	MD03G1290900.v1.1 MD03G1291000.v1.1 MD08G1195600.v1.1 MD11G1307400.v1.1 MD11G1307500.v1.1
prunus_persica	Prupe.1G529400_v2.0.a1 Prupe.1G529500_v2.0.a1 Prupe.8G263900_v2.0.a1 Prupe.8G264000_v2.0.a1
pyrus_communis	pycom08g16800
rosa_chinensis	RchiOBHm_Chr6g0294321 RchiOBHm_Chr6g0294331 RchiOBHm_Chr7g0236951 RchiOBHm_Chr7g0236971 RchiOBHm_Chr7g0237041 RchiOBHm_Chr7g0237051
rosa_laevigata	RLG00000001034 RLG00000001035 RLG00000001039 RLG00000001043 RLG00000001045 RLG00000011880 RLG00000011881 RLG00000034181
rosa_multiflora	Rmu_co8086188.1_g000001 Rmu_co8458485.1_g000001 Rmu_sc0000272.1_g000007 Rmu_sc0000272.1_g000009 Rmu_sc0002131.1_g000030 Rmu_sc0002131.1_g000037 Rmu_sc0005369.1_g000005 Rmu_sc0005369.1_g000008 Rmu_sc0007991.1_g000005 Rmu_sc0007991.1_g000013 Rmu_sc0033563.1_g000001
rosa_roxburghii	Rroxscaffold_3G00224540 Rroxscaffold_3G00224600 Rroxscaffold_3G00224650 Rroxscaffold_3G00224660 Rroxscaffold_7G00173470 Rroxscaffold_7G00173480
rosa_rugosa	Rorug06G0243400 Rorug06G0243500 Rorug07G0301200 Rorug07G0301500 Rorug07G0301600
rosa_samantha	Rh6AG356000 Rh6AG356100 Rh6BG362600 Rh6BG362800 Rh6CG370000 Rh6CG370100 Rh6DG356500 Rh6DG356600 Rh7AG456200 Rh7AG456500 Rh7AG457500 Rh7AG457600 Rh7BG427500 Rh7BG427700 Rh7BG428400 Rh7BG428500 Rh7CG475100 Rh7CG475300 Rh7CG475700 Rh7CG475800 Rh7DG444100 Rh7DG444300 Rh7DG444700 Rh7DG444900
rosa_wichuraiana	Rw6G031020 Rw6G031030 Rw7G037870 Rw7G037880 Rw7G037920 Rw7G037940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1127
AatII	GACGTC	1 cut(s)	423
Acc36I	ACCTGC	1 cut(s)	86
Acc65I	GGTACC	1 cut(s)	1074
AccB1I	GGYRCC	2 cut(s)	586, 1074
AccI	GTMKAC	1 cut(s)	1131
AccII	CGCG	1 cut(s)	215
AccIII	TCCGGA	1 cut(s)	219
AciI	CCGC	7 cut(s)	215, 300, 747, 985, 993, 1047, 1166
AclWI	GGATC	3 cut(s)	104, 1270, 1498
AcoI	YGGCCR	2 cut(s)	205, 1517
AcsI	RAATTY	2 cut(s)	137, 661
AcuI	CTGAAG	1 cut(s)	641
AcyI	GRCGYC	1 cut(s)	420
AfaI	GTAC	3 cut(s)	477, 1076, 1379
AfiI	CCNNNNNNNGG	4 cut(s)	214, 1211, 1607, 1622
AgsI	TTSAA	6 cut(s)	28, 317, 928, 1222, 1368, 1537
AhlI	ACTAGT	1 cut(s)	1499
AjiI	CACGTC	1 cut(s)	673
AjnI	CCWGG	4 cut(s)	428, 1331, 1486, 1519
AleI	CACNNNNGTG	1 cut(s)	1284
Alw26I	GTCTC	2 cut(s)	680, 1113
AlwI	GGATC	3 cut(s)	104, 1270, 1498
AlwNI	CAGNNNCTG	3 cut(s)	740, 1122, 1169
Aor13HI	TCCGGA	1 cut(s)	219
AoxI	GGCC	4 cut(s)	205, 1349, 1483, 1517
ApaI	GGGCCC	1 cut(s)	1487
ApeKI	GCWGC	6 cut(s)	86, 652, 749, 1062, 1163, 1597
ApoI	RAATTY	2 cut(s)	137, 661
ArsI	GACNNNNNNTTYG	2 cut(s)	1136, 1168
Asp700I	GAANNNNTTC	1 cut(s)	1388
Asp718I	GGTACC	1 cut(s)	1074
AspS9I	GGNCC	3 cut(s)	217, 1483, 1484
AsuC2I	CCSGG	1 cut(s)	1198
AsuHPI	GGTGA	6 cut(s)	271, 643, 680, 691, 715, 875
AsuII	TTCGAA	1 cut(s)	1068
AvaII	GGWCC	1 cut(s)	217
BaeGI	GKGCMC	1 cut(s)	1487
BalI	TGGCCA	1 cut(s)	1519
BanI	GGYRCC	2 cut(s)	586, 1074
BanII	GRGCYC	1 cut(s)	1487
BbsI	GAAGAC	1 cut(s)	609
BbvI	GCAGC	6 cut(s)	73, 639, 761, 1074, 1175, 1584
BccI	CCATC	5 cut(s)	68, 464, 576, 905, 1391
BceAI	ACGGC	6 cut(s)	192, 220, 665, 1001, 1007, 1248
BciT130I	CCWGG	4 cut(s)	430, 1333, 1488, 1521
BciVI	GTATCC	1 cut(s)	127
BclI	TGATCA	1 cut(s)	633
BcnI	CCSGG	1 cut(s)	1198
BcoDI	GTCTC	2 cut(s)	680, 1113
BcuI	ACTAGT	1 cut(s)	1499
BfaI	CTAG	3 cut(s)	563, 1343, 1500
BfmI	CTRYAG	2 cut(s)	87, 1114
BfuAI	ACCTGC	1 cut(s)	86
BfuI	GTATCC	1 cut(s)	127
BglI	GCCNNNNNGGC	1 cut(s)	1232
BisI	GCNGC	8 cut(s)	87, 653, 747, 750, 1063, 1164, 1167, 1598
BlpI	GCTNAGC	1 cut(s)	1545
BlsI	GCNGC	8 cut(s)	88, 654, 748, 751, 1064, 1165, 1168, 1599
Bme1390I	CCNGG	5 cut(s)	430, 1198, 1333, 1488, 1521
Bme18I	GGWCC	1 cut(s)	217
BmgBI	CACGTC	1 cut(s)	673
BmgT120I	GGNCC	3 cut(s)	217, 1483, 1484
BmiI	GGNNCC	3 cut(s)	588, 1076, 1485
BmrFI	CCNGG	5 cut(s)	430, 1198, 1333, 1488, 1521
BmsI	GCATC	2 cut(s)	879, 1197
BpiI	GAAGAC	1 cut(s)	609
BpmI	CTGGAG	2 cut(s)	1455, 1629
Bpu10I	CCTNAGC	1 cut(s)	1236
Bpu1102I	GCTNAGC	1 cut(s)	1545
Bpu14I	TTCGAA	1 cut(s)	1068
BpuMI	CCSGG	1 cut(s)	1198
BsaBI	GATNNNNATC	1 cut(s)	794
BsaHI	GRCGYC	1 cut(s)	420
BsaJI	CCNNGG	5 cut(s)	414, 956, 1196, 1352, 1486
BsaWI	WCCGGW	3 cut(s)	219, 754, 1622
Bsc4I	CCNNNNNNNGG	4 cut(s)	214, 1211, 1607, 1622
Bse118I	RCCGGY	1 cut(s)	1265
Bse1I	ACTGG	3 cut(s)	113, 1438, 1612
Bse3DI	GCAATG	1 cut(s)	828
Bse8I	GATNNNNATC	1 cut(s)	794
BseAI	TCCGGA	1 cut(s)	219
BseBI	CCWGG	4 cut(s)	430, 1333, 1488, 1521
BseDI	CCNNGG	5 cut(s)	414, 956, 1196, 1352, 1486
BseGI	GGATG	4 cut(s)	60, 475, 587, 1402
BseJI	GATNNNNATC	1 cut(s)	794
BseLI	CCNNNNNNNGG	4 cut(s)	214, 1211, 1607, 1622
BseMI	GCAATG	1 cut(s)	828
BseMII	CTCAG	2 cut(s)	448, 1250
BseNI	ACTGG	3 cut(s)	113, 1438, 1612
BseRI	GAGGAG	4 cut(s)	520, 623, 632, 635
BseSI	GKGCMC	1 cut(s)	1487
BseX3I	CGGCCG	1 cut(s)	205
BseXI	GCAGC	6 cut(s)	73, 639, 761, 1074, 1175, 1584
BseYI	CCCAGC	1 cut(s)	401
Bsh1236I	CGCG	1 cut(s)	215
Bsh1285I	CGRYCG	1 cut(s)	208
BshFI	GGCC	4 cut(s)	207, 1351, 1485, 1519
BshNI	GGYRCC	2 cut(s)	586, 1074
BsiEI	CGRYCG	1 cut(s)	208
BsiSI	CCGG	6 cut(s)	220, 755, 996, 1198, 1266, 1623
BslI	CCNNNNNNNGG	4 cut(s)	214, 1211, 1607, 1622
BsmAI	GTCTC	2 cut(s)	680, 1113
BsmI	GAATGC	1 cut(s)	1049
BsnI	GGCC	4 cut(s)	207, 1351, 1485, 1519
Bsp119I	TTCGAA	1 cut(s)	1068
Bsp120I	GGGCCC	1 cut(s)	1483
Bsp1286I	GDGCHC	1 cut(s)	1487
Bsp13I	TCCGGA	1 cut(s)	219
Bsp1407I	TGTACA	1 cut(s)	1377
Bsp143I	GATC	5 cut(s)	77, 109, 633, 1275, 1490
Bsp1720I	GCTNAGC	1 cut(s)	1545
Bsp19I	CCATGG	2 cut(s)	956, 1352
BspACI	CCGC	7 cut(s)	215, 300, 747, 985, 993, 1047, 1166
BspANI	GGCC	4 cut(s)	207, 1351, 1485, 1519
BspCNI	CTCAG	2 cut(s)	447, 1249
BspEI	TCCGGA	1 cut(s)	219
BspFNI	CGCG	1 cut(s)	215
BspLI	GGNNCC	3 cut(s)	588, 1076, 1485
BspMAI	CTGCAG	1 cut(s)	91
BspMI	ACCTGC	1 cut(s)	86
BspPI	GGATC	3 cut(s)	104, 1270, 1498
BspT104I	TTCGAA	1 cut(s)	1068
BspT107I	GGYRCC	2 cut(s)	586, 1074
BsrDI	GCAATG	1 cut(s)	828
BsrFI	RCCGGY	1 cut(s)	1265
BsrGI	TGTACA	1 cut(s)	1377
BsrI	ACTGG	3 cut(s)	113, 1438, 1612
BssAI	RCCGGY	1 cut(s)	1265
BssECI	CCNNGG	5 cut(s)	414, 956, 1196, 1352, 1486
BssMI	GATC	5 cut(s)	77, 109, 633, 1275, 1490
BssNI	GRCGYC	1 cut(s)	420
BssT1I	CCWWGG	2 cut(s)	956, 1352
Bst2UI	CCWGG	4 cut(s)	430, 1333, 1488, 1521
Bst4CI	ACNGT	8 cut(s)	691, 697, 883, 1034, 1141, 1286, 1375, 1567
Bst6I	CTCTTC	2 cut(s)	810, 1501
BstACI	GRCGYC	1 cut(s)	420
BstAUI	TGTACA	1 cut(s)	1377
BstBI	TTCGAA	1 cut(s)	1068
BstC8I	GCNNGC	3 cut(s)	326, 361, 1551
BstDEI	CTNAG	3 cut(s)	434, 1236, 1545
BstDSI	CCRYGG	2 cut(s)	956, 1352
BstEII	GGTNACC	1 cut(s)	1523
BstF5I	GGATG	4 cut(s)	60, 475, 587, 1402
BstFNI	CGCG	1 cut(s)	215
BstKTI	GATC	5 cut(s)	80, 112, 636, 1278, 1493
BstMAI	GTCTC	2 cut(s)	680, 1113
BstMBI	GATC	5 cut(s)	77, 109, 633, 1275, 1490
BstMCI	CGRYCG	1 cut(s)	208
BstMWI	GCNNNNNNNGC	7 cut(s)	92, 308, 471, 746, 1207, 1232, 1550
BstNI	CCWGG	4 cut(s)	430, 1333, 1488, 1521
BstNSI	RCATGY	1 cut(s)	465
BstPI	GGTNACC	1 cut(s)	1523
BstSCI	CCNGG	5 cut(s)	428, 1196, 1331, 1486, 1519
BstSFI	CTRYAG	2 cut(s)	87, 1114
BstSLI	GKGCMC	1 cut(s)	1487
BstUI	CGCG	1 cut(s)	215
BstV1I	GCAGC	6 cut(s)	73, 639, 761, 1074, 1175, 1584
BstV2I	GAAGAC	1 cut(s)	609
BstZI	CGGCCG	1 cut(s)	205
BsuI	GTATCC	1 cut(s)	127
BsuRI	GGCC	4 cut(s)	207, 1351, 1485, 1519
BtgI	CCRYGG	2 cut(s)	956, 1352
BtrI	CACGTC	1 cut(s)	673
BtsCI	GGATG	4 cut(s)	60, 475, 587, 1402
BtsI	GCAGTG	1 cut(s)	975
BtsIMutI	CAGTG	5 cut(s)	442, 693, 975, 1146, 1572
BveI	ACCTGC	1 cut(s)	86
Cac8I	GCNNGC	3 cut(s)	326, 361, 1551
CaiI	CAGNNNCTG	3 cut(s)	740, 1122, 1169
Cfr10I	RCCGGY	1 cut(s)	1265
Cfr13I	GGNCC	3 cut(s)	217, 1483, 1484
CpoI	CGGWCCG	1 cut(s)	217
CseI	GACGC	1 cut(s)	746
Csp6I	GTAC	3 cut(s)	476, 1075, 1378
CspCI	CAANNNNNGTGG	2 cut(s)	924, 959
CspI	CGGWCCG	1 cut(s)	217
CviAII	CATG	8 cut(s)	169, 462, 639, 957, 1353, 1381, 1393, 1515
CviQI	GTAC	3 cut(s)	476, 1075, 1378
DdeI	CTNAG	3 cut(s)	434, 1236, 1545
DpnI	GATC	5 cut(s)	79, 111, 635, 1277, 1492
DpnII	GATC	5 cut(s)	77, 109, 633, 1275, 1490
DraI	TTTAAA	1 cut(s)	774
DrdI	GACNNNNNNGTC	1 cut(s)	1127
DseDI	GACNNNNNNGTC	1 cut(s)	1127
EaeI	YGGCCR	2 cut(s)	205, 1517
EagI	CGGCCG	1 cut(s)	205
Eam1104I	CTCTTC	2 cut(s)	810, 1501
EarI	CTCTTC	2 cut(s)	810, 1501
EclXI	CGGCCG	1 cut(s)	205
Eco130I	CCWWGG	2 cut(s)	956, 1352
Eco24I	GRGCYC	1 cut(s)	1487
Eco47I	GGWCC	1 cut(s)	217
Eco52I	CGGCCG	1 cut(s)	205
Eco57I	CTGAAG	1 cut(s)	641
Eco91I	GGTNACC	1 cut(s)	1523
EcoO109I	RGGNCCY	2 cut(s)	1483, 1484
EcoO65I	GGTNACC	1 cut(s)	1523
EcoRI	GAATTC	1 cut(s)	137
EcoRII	CCWGG	4 cut(s)	428, 1331, 1486, 1519
EcoT14I	CCWWGG	2 cut(s)	956, 1352
EcoT38I	GRGCYC	1 cut(s)	1487
ErhI	CCWWGG	2 cut(s)	956, 1352
FaeI	CATG	8 cut(s)	172, 465, 642, 960, 1356, 1384, 1396, 1518
FatI	CATG	8 cut(s)	168, 461, 638, 956, 1352, 1380, 1392, 1514
FbaI	TGATCA	1 cut(s)	633
FblI	GTMKAC	1 cut(s)	1131
Fnu4HI	GCNGC	8 cut(s)	87, 653, 747, 750, 1063, 1164, 1167, 1598
FokI	GGATG	4 cut(s)	47, 482, 594, 1409
FriOI	GRGCYC	1 cut(s)	1487
Fsp4HI	GCNGC	8 cut(s)	87, 653, 747, 750, 1063, 1164, 1167, 1598
FspBI	CTAG	3 cut(s)	563, 1343, 1500
GluI	GCNGC	8 cut(s)	87, 653, 747, 750, 1063, 1164, 1167, 1598
GsaI	CCCAGC	1 cut(s)	405
GsuI	CTGGAG	2 cut(s)	1455, 1629
HaeIII	GGCC	4 cut(s)	207, 1351, 1485, 1519
HapII	CCGG	6 cut(s)	220, 755, 996, 1198, 1266, 1623
HgaI	GACGC	1 cut(s)	746
Hin1I	GRCGYC	1 cut(s)	420
Hin1II	CATG	8 cut(s)	172, 465, 642, 960, 1356, 1384, 1396, 1518
HincII	GTYRAC	1 cut(s)	760
HindII	GTYRAC	1 cut(s)	760
HindIII	AAGCTT	1 cut(s)	326
HinfI	GANTC	4 cut(s)	37, 286, 700, 1255
HpaI	GTTAAC	1 cut(s)	760
HpaII	CCGG	6 cut(s)	220, 755, 996, 1198, 1266, 1623
HphI	GGTGA	6 cut(s)	271, 643, 680, 691, 715, 875
Hpy188I	TCNGA	8 cut(s)	660, 852, 900, 1028, 1206, 1295, 1303, 1318
Hpy188III	TCNNGA	5 cut(s)	75, 220, 574, 704, 787
Hpy99I	CGWCG	1 cut(s)	1022
HpyAV	CCTTC	8 cut(s)	162, 571, 607, 991, 1064, 1351, 1399, 1409
HpyCH4III	ACNGT	8 cut(s)	691, 697, 883, 1034, 1141, 1286, 1375, 1567
HpyCH4IV	ACGT	4 cut(s)	345, 420, 672, 1128
HpyF10VI	GCNNNNNNNGC	7 cut(s)	92, 308, 471, 746, 1207, 1232, 1550
HpyF3I	CTNAG	3 cut(s)	434, 1236, 1545
HpySE526I	ACGT	4 cut(s)	345, 420, 672, 1128
Hsp92I	GRCGYC	1 cut(s)	420
Hsp92II	CATG	8 cut(s)	172, 465, 642, 960, 1356, 1384, 1396, 1518
Kpn2I	TCCGGA	1 cut(s)	219
KpnI	GGTACC	1 cut(s)	1078
Ksp22I	TGATCA	1 cut(s)	633
KspAI	GTTAAC	1 cut(s)	760
Kzo9I	GATC	5 cut(s)	77, 109, 633, 1275, 1490
LmnI	GCTCC	3 cut(s)	368, 757, 998
Lsp1109I	GCAGC	6 cut(s)	73, 639, 761, 1074, 1175, 1584
LweI	GCATC	2 cut(s)	879, 1197
MaeI	CTAG	3 cut(s)	563, 1343, 1500
MaeII	ACGT	4 cut(s)	345, 420, 672, 1128
MaeIII	GTNAC	7 cut(s)	209, 668, 691, 779, 1184, 1510, 1523
MalI	GATC	5 cut(s)	79, 111, 635, 1277, 1492
MboI	GATC	5 cut(s)	77, 109, 633, 1275, 1490
MboII	GAAGA	8 cut(s)	38, 329, 480, 483, 614, 827, 1518, 1600
MhlI	GDGCHC	1 cut(s)	1487
MlsI	TGGCCA	1 cut(s)	1519
MluCI	AATT	7 cut(s)	137, 245, 661, 928, 948, 1057, 1109
MluNI	TGGCCA	1 cut(s)	1519
MlyI	GAGTC	3 cut(s)	31, 280, 709
MmeI	TCCRAC	3 cut(s)	298, 558, 1400
Mox20I	TGGCCA	1 cut(s)	1519
MroI	TCCGGA	1 cut(s)	219
MroXI	GAANNNNTTC	1 cut(s)	1388
MscI	TGGCCA	1 cut(s)	1519
MseI	TTAA	3 cut(s)	759, 773, 1448
MslI	CAYNNNNRTG	1 cut(s)	1284
Msp20I	TGGCCA	1 cut(s)	1519
MspA1I	CMGCKG	1 cut(s)	1166
MspI	CCGG	6 cut(s)	220, 755, 996, 1198, 1266, 1623
MspR9I	CCNGG	5 cut(s)	430, 1198, 1333, 1488, 1521
Mva1269I	GAATGC	1 cut(s)	1049
MvaI	CCWGG	4 cut(s)	430, 1333, 1488, 1521
MvnI	CGCG	1 cut(s)	215
MwoI	GCNNNNNNNGC	7 cut(s)	92, 308, 471, 746, 1207, 1232, 1550
NciI	CCSGG	1 cut(s)	1198
NcoI	CCATGG	2 cut(s)	956, 1352
NdeII	GATC	5 cut(s)	77, 109, 633, 1275, 1490
NlaIII	CATG	8 cut(s)	172, 465, 642, 960, 1356, 1384, 1396, 1518
NlaIV	GGNNCC	3 cut(s)	588, 1076, 1485
NmeAIII	GCCGAG	1 cut(s)	439
NmuCI	GTSAC	2 cut(s)	668, 691
NspI	RCATGY	1 cut(s)	465
NspV	TTCGAA	1 cut(s)	1068
OliI	CACNNNNGTG	1 cut(s)	1284
PctI	GAATGC	1 cut(s)	1049
PdmI	GAANNNNTTC	1 cut(s)	1388
PfeI	GAWTC	1 cut(s)	1255
PkrI	GCNGC	8 cut(s)	88, 654, 748, 751, 1064, 1165, 1168, 1599
PleI	GAGTC	3 cut(s)	31, 280, 708
PpsI	GAGTC	3 cut(s)	31, 280, 708
Psp6I	CCWGG	4 cut(s)	428, 1331, 1486, 1519
PspEI	GGTNACC	1 cut(s)	1523
PspFI	CCCAGC	1 cut(s)	401
PspGI	CCWGG	4 cut(s)	428, 1331, 1486, 1519
PspN4I	GGNNCC	3 cut(s)	588, 1076, 1485
PspOMI	GGGCCC	1 cut(s)	1483
PspPI	GGNCC	3 cut(s)	217, 1483, 1484
PstI	CTGCAG	1 cut(s)	91
PstNI	CAGNNNCTG	3 cut(s)	740, 1122, 1169
RsaI	GTAC	3 cut(s)	477, 1076, 1379
RsaNI	GTAC	3 cut(s)	476, 1075, 1378
RseI	CAYNNNNRTG	1 cut(s)	1284
Rsr2I	CGGWCCG	1 cut(s)	217
RsrII	CGGWCCG	1 cut(s)	217
SaqAI	TTAA	3 cut(s)	759, 773, 1448
SatI	GCNGC	8 cut(s)	87, 653, 747, 750, 1063, 1164, 1167, 1598
Sau3AI	GATC	5 cut(s)	77, 109, 633, 1275, 1490
Sau96I	GGNCC	3 cut(s)	217, 1483, 1484
SchI	GAGTC	3 cut(s)	31, 280, 709
ScrFI	CCNGG	5 cut(s)	430, 1198, 1333, 1488, 1521
SduI	GDGCHC	1 cut(s)	1487
SfaNI	GCATC	2 cut(s)	879, 1197
SfcI	CTRYAG	2 cut(s)	87, 1114
SfuI	TTCGAA	1 cut(s)	1068
SinI	GGWCC	1 cut(s)	217
SmiMI	CAYNNNNRTG	1 cut(s)	1284
SpeI	ACTAGT	1 cut(s)	1499
Sse9I	AATT	7 cut(s)	137, 245, 661, 928, 948, 1057, 1109
SsiI	CCGC	7 cut(s)	215, 300, 747, 985, 993, 1047, 1166
SspMI	CTAG	3 cut(s)	563, 1343, 1500
StyD4I	CCNGG	5 cut(s)	428, 1196, 1331, 1486, 1519
StyI	CCWWGG	2 cut(s)	956, 1352
TaaI	ACNGT	8 cut(s)	691, 697, 883, 1034, 1141, 1286, 1375, 1567
TaiI	ACGT	4 cut(s)	348, 423, 675, 1131
TaqI	TCGA	7 cut(s)	35, 76, 284, 407, 1068, 1243, 1493
TasI	AATT	7 cut(s)	137, 245, 661, 928, 948, 1057, 1109
TatI	WGTACW	1 cut(s)	1377
TauI	GCSGC	2 cut(s)	749, 1169
TfiI	GAWTC	1 cut(s)	1255
Tru1I	TTAA	3 cut(s)	759, 773, 1448
Tru9I	TTAA	3 cut(s)	759, 773, 1448
TscAI	CASTG	5 cut(s)	442, 700, 975, 1146, 1572
TseFI	GTSAC	2 cut(s)	668, 691
TseI	GCWGC	6 cut(s)	86, 652, 749, 1062, 1163, 1597
Tsp45I	GTSAC	2 cut(s)	668, 691
TspDTI	ATGAA	8 cut(s)	38, 144, 483, 527, 828, 1138, 1381, 1397
TspGWI	ACGGA	2 cut(s)	723, 1149
TspRI	CASTG	5 cut(s)	442, 700, 975, 1146, 1572
VpaK11BI	GGWCC	1 cut(s)	217
XapI	RAATTY	2 cut(s)	137, 661
XceI	RCATGY	1 cut(s)	465
XmiI	GTMKAC	1 cut(s)	1131
XmnI	GAANNNNTTC	1 cut(s)	1388
XspI	CTAG	3 cut(s)	563, 1343, 1500
ZraI	GACGTC	1 cut(s)	421

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.