AT1G25370

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002115
Physical Location & Seq
Reverse (-)
8897564 .. 8899111
1548 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G25370.1

Sequence Viewer

Length: 792 bp
ATGGTATTTCCCGGTGAGATGACGACGACGACAACGAGACCTGAGCAACGATCGAAGACTCTTCACAATTTCCCTCTCCCGAACTTATGGGGGAATCAGAGACAACTCAAGTGCACGAAGATCGACTCTATCAGCAACAACAACAACAACGGAGGAGGTCCCGGCGGCGATCACCGACTACGACGCCGATCTCCGCCTCTGGAATTCGCTGATTCTCCCGTTTCGATGCCGTTTCGATTCGGTAACTCCGATCATCGCCGACCGTTTAAATCGGGAAGCGAAGAAGGGATCGAAGAGTTCAGGGTGAAGCTTATGTCGGATCTGAAGACGGAGACAGATAAGATCACACAATCGATGTTTAACAAAGGCGTAACCGAAGAGGAAGAGGAACAAATCGACGGTTCTGGTTCTGGTTCTGGTTCCGGTCAAGAGAAGGAGATGATTCCTCCGGTGAAGCCGTGGAATCTAAGGAAAAGAAGAGCAGCAGCGTGTAAAGAACCGGAATCCAACAGTTTGATCAATAAAGGAATTGTGATTGAGGAGAAGGTAGTGAAGAATCCATCTCCGGTGAGAGGCGGCGGCGGAGTGGTCGAAGCGGAGAAGAAGCGTCCTATGTTCTCTATGAAGCTATCGAAGAAGGAGATGGAAGAAGATTTCATTGGGATGGTAGGTCACCGAGCACCGCGTCGGCCCAAGAAACGGTCCAAAACTGTTCAGAAAAAACTCGATAGTTTGTTTCCAGGGTTGTATTTGACTGAAGTGACACATGATGCATATAAGGTTCCAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

263

Amino Acids

29.55

Weight (kDa)

9.55

Isoelectric Point (pI)

77.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1639 PF07797 210 - 259 2e-26 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 685
AciI CCGC 7 cut(s) 165, 194, 576, 579, 582, 596, 683
AclWI GGATC 2 cut(s) 296, 327
AcsI RAATTY 1 cut(s) 203
AcuI CTGAAG 2 cut(s) 344, 777
AcyI GRCGYC 1 cut(s) 184
AfiI CCNNNNNNNGG 2 cut(s) 572, 699
AjnI CCWGG 1 cut(s) 739
AjuI GAANNNNNNNTTGG 2 cut(s) 642, 674
AluBI AGCT 2 cut(s) 310, 628
AluI AGCT 2 cut(s) 310, 628
Alw21I GWGCWC 2 cut(s) 116, 682
Alw26I GTCTC 3 cut(s) 31, 94, 326
Alw44I GTGCAC 1 cut(s) 112
AlwI GGATC 2 cut(s) 296, 327
AoxI GGCC 1 cut(s) 689
ApaLI GTGCAC 1 cut(s) 112
ApeKI GCWGC 2 cut(s) 482, 485
ApoI RAATTY 1 cut(s) 203
AspS9I GGNCC 3 cut(s) 158, 690, 702
AsuC2I CCSGG 2 cut(s) 12, 162
AsuHPI GGTGA 6 cut(s) 26, 164, 316, 463, 580, 665
AvaII GGWCC 2 cut(s) 158, 702
BaeGI GKGCMC 1 cut(s) 116
BbsI GAAGAC 2 cut(s) 62, 332
Bbv12I GWGCWC 2 cut(s) 116, 682
BbvI GCAGC 2 cut(s) 494, 497
BccI CCATC 3 cut(s) 568, 637, 658
BceAI ACGGC 2 cut(s) 214, 442
BcgI CGANNNNNNTGC 2 cut(s) 103, 137
BciT130I CCWGG 1 cut(s) 741
BclI TGATCA 1 cut(s) 516
BcnI CCSGG 2 cut(s) 12, 162
BcoDI GTCTC 3 cut(s) 31, 94, 326
BisI GCNGC 5 cut(s) 166, 483, 486, 577, 580
BlsI GCNGC 5 cut(s) 167, 484, 487, 578, 581
Bme1390I CCNGG 3 cut(s) 12, 162, 741
Bme18I GGWCC 2 cut(s) 158, 702
BmgT120I GGNCC 3 cut(s) 158, 690, 702
BmiI GGNNCC 3 cut(s) 160, 421, 783
BmrFI CCNGG 3 cut(s) 12, 162, 741
BmsI GCATC 2 cut(s) 216, 760
BpiI GAAGAC 2 cut(s) 62, 332
Bpu10I CCTNAGC 1 cut(s) 42
BpuEI CTTGAG 1 cut(s) 92
BpuMI CCSGG 2 cut(s) 12, 162
Bsa29I ATCGAT 1 cut(s) 353
BsaHI GRCGYC 1 cut(s) 184
BsaI GGTCTC 1 cut(s) 31
BsaJI CCNNGG 2 cut(s) 458, 740
BsaWI WCCGGW 4 cut(s) 422, 448, 499, 565
Bsc4I CCNNNNNNNGG 2 cut(s) 572, 699
BseBI CCWGG 1 cut(s) 741
BseCI ATCGAT 1 cut(s) 353
BseDI CCNNGG 2 cut(s) 458, 740
BseGI GGATG 1 cut(s) 669
BseLI CCNNNNNNNGG 2 cut(s) 572, 699
BseMII CTCAG 1 cut(s) 33
BseRI GAGGAG 2 cut(s) 168, 554
BseSI GKGCMC 1 cut(s) 116
BseXI GCAGC 2 cut(s) 494, 497
Bsh1236I CGCG 1 cut(s) 685
Bsh1285I CGRYCG 2 cut(s) 53, 263
BshFI GGCC 1 cut(s) 691
BshVI ATCGAT 1 cut(s) 353
BsiEI CGRYCG 2 cut(s) 53, 263
BsiHKAI GWGCWC 2 cut(s) 116, 682
BsiSI CCGG 6 cut(s) 12, 162, 423, 449, 500, 566
BslFI GGGAC 1 cut(s) 144
BslI CCNNNNNNNGG 2 cut(s) 572, 699
BsmAI GTCTC 3 cut(s) 31, 94, 326
BsmFI GGGAC 1 cut(s) 144
BsnI GGCC 1 cut(s) 691
Bso31I GGTCTC 1 cut(s) 31
Bsp1286I GDGCHC 2 cut(s) 116, 682
Bsp143I GATC 9 cut(s) 50, 120, 169, 188, 250, 288, 319, 342, 516
BspACI CCGC 7 cut(s) 165, 194, 576, 579, 582, 596, 683
BspANI GGCC 1 cut(s) 691
BspCNI CTCAG 1 cut(s) 34
BspDI ATCGAT 1 cut(s) 353
BspFNI CGCG 1 cut(s) 685
BspLI GGNNCC 3 cut(s) 160, 421, 783
BspPI GGATC 2 cut(s) 296, 327
BspQI GCTCTTC 1 cut(s) 472
BspTNI GGTCTC 1 cut(s) 31
BssECI CCNNGG 2 cut(s) 458, 740
BssMI GATC 9 cut(s) 50, 120, 169, 188, 250, 288, 319, 342, 516
BssNI GRCGYC 1 cut(s) 184
Bst2UI CCWGG 1 cut(s) 741
Bst4CI ACNGT 5 cut(s) 264, 401, 512, 702, 712
Bst6I CTCTTC 5 cut(s) 66, 288, 372, 378, 472
BstACI GRCGYC 1 cut(s) 184
BstDEI CTNAG 2 cut(s) 42, 467
BstDSI CCRYGG 1 cut(s) 458
BstEII GGTNACC 1 cut(s) 671
BstF5I GGATG 1 cut(s) 669
BstFNI CGCG 1 cut(s) 685
BstKTI GATC 9 cut(s) 53, 123, 172, 191, 253, 291, 322, 345, 519
BstMAI GTCTC 3 cut(s) 31, 94, 326
BstMBI GATC 9 cut(s) 50, 120, 169, 188, 250, 288, 319, 342, 516
BstMCI CGRYCG 2 cut(s) 53, 263
BstNI CCWGG 1 cut(s) 741
BstPI GGTNACC 1 cut(s) 671
BstSCI CCNGG 3 cut(s) 10, 160, 739
BstSLI GKGCMC 1 cut(s) 116
BstUI CGCG 1 cut(s) 685
BstV1I GCAGC 2 cut(s) 494, 497
BstV2I GAAGAC 2 cut(s) 62, 332
BstX2I RGATCY 1 cut(s) 319
BstYI RGATCY 1 cut(s) 319
Bsu15I ATCGAT 1 cut(s) 353
BsuRI GGCC 1 cut(s) 691
BsuTUI ATCGAT 1 cut(s) 353
BtgI CCRYGG 1 cut(s) 458
BtgZI GCGATG 1 cut(s) 239
BtsCI GGATG 1 cut(s) 669
Cfr13I GGNCC 3 cut(s) 158, 690, 702
ClaI ATCGAT 1 cut(s) 353
CseI GACGC 3 cut(s) 192, 596, 674
CviAII CATG 1 cut(s) 767
CviJI RGCY 4 cut(s) 310, 457, 628, 691
CviKI_1 RGCY 4 cut(s) 310, 457, 628, 691
DdeI CTNAG 2 cut(s) 42, 467
DpnI GATC 9 cut(s) 52, 122, 171, 190, 252, 290, 321, 344, 518
DpnII GATC 9 cut(s) 50, 120, 169, 188, 250, 288, 319, 342, 516
DraI TTTAAA 1 cut(s) 268
Eam1104I CTCTTC 5 cut(s) 66, 288, 372, 378, 472
EarI CTCTTC 5 cut(s) 66, 288, 372, 378, 472
EciI GGCGGA 2 cut(s) 183, 597
Eco31I GGTCTC 1 cut(s) 31
Eco47I GGWCC 2 cut(s) 158, 702
Eco57I CTGAAG 2 cut(s) 344, 777
Eco91I GGTNACC 1 cut(s) 671
EcoO109I RGGNCCY 1 cut(s) 158
EcoO65I GGTNACC 1 cut(s) 671
EcoRI GAATTC 1 cut(s) 203
EcoRII CCWGG 1 cut(s) 739
EcoT22I ATGCAT 1 cut(s) 775
FaeI CATG 1 cut(s) 770
FaiI YATR 7 cut(s) 88, 314, 614, 623, 768, 775, 777
FaqI GGGAC 1 cut(s) 144
FatI CATG 1 cut(s) 766
FbaI TGATCA 1 cut(s) 516
Fnu4HI GCNGC 5 cut(s) 166, 483, 486, 577, 580
FokI GGATG 1 cut(s) 676
Fsp4HI GCNGC 5 cut(s) 166, 483, 486, 577, 580
GluI GCNGC 5 cut(s) 166, 483, 486, 577, 580
HaeIII GGCC 1 cut(s) 691
HapII CCGG 6 cut(s) 12, 162, 423, 449, 500, 566
HgaI GACGC 3 cut(s) 192, 596, 674
Hin1I GRCGYC 1 cut(s) 184
Hin1II CATG 1 cut(s) 770
HindIII AAGCTT 1 cut(s) 308
HinfI GANTC 9 cut(s) 58, 94, 125, 212, 237, 442, 463, 503, 556
HpaII CCGG 6 cut(s) 12, 162, 423, 449, 500, 566
HphI GGTGA 6 cut(s) 26, 164, 316, 463, 580, 665
Hpy166II GTNNAC 1 cut(s) 114
Hpy188I TCNGA 5 cut(s) 99, 250, 319, 324, 717
Hpy188III TCNNGA 5 cut(s) 79, 200, 273, 428, 785
Hpy8I GTNNAC 1 cut(s) 114
Hpy99I CGWCG 5 cut(s) 28, 31, 186, 401, 690
HpyAV CCTTC 4 cut(s) 278, 427, 538, 631
HpyCH4III ACNGT 5 cut(s) 264, 401, 512, 702, 712
HpyCH4V TGCA 2 cut(s) 114, 773
HpyF3I CTNAG 2 cut(s) 42, 467
Hsp92I GRCGYC 1 cut(s) 184
Hsp92II CATG 1 cut(s) 770
Ksp22I TGATCA 1 cut(s) 516
Kzo9I GATC 9 cut(s) 50, 120, 169, 188, 250, 288, 319, 342, 516
LguI GCTCTTC 1 cut(s) 472
Lsp1109I GCAGC 2 cut(s) 494, 497
LweI GCATC 2 cut(s) 216, 760
MaeIII GTNAC 4 cut(s) 242, 370, 671, 760
MalI GATC 9 cut(s) 52, 122, 171, 190, 252, 290, 321, 344, 518
MboI GATC 9 cut(s) 50, 120, 169, 188, 250, 288, 319, 342, 516
MflI RGATCY 1 cut(s) 319
MhlI GDGCHC 2 cut(s) 116, 682
MluCI AATT 3 cut(s) 67, 203, 528
MlyI GAGTC 2 cut(s) 52, 119
MmeI TCCRAC 2 cut(s) 297, 531
MnlI CCTC 9 cut(s) 84, 146, 149, 207, 373, 379, 456, 532, 566
Mph1103I ATGCAT 1 cut(s) 775
MseI TTAA 2 cut(s) 267, 360
MslI CAYNNNNRTG 1 cut(s) 662
MspI CCGG 6 cut(s) 12, 162, 423, 449, 500, 566
MspR9I CCNGG 3 cut(s) 12, 162, 741
MvaI CCWGG 1 cut(s) 741
MvnI CGCG 1 cut(s) 685
NciI CCSGG 2 cut(s) 12, 162
NdeII GATC 9 cut(s) 50, 120, 169, 188, 250, 288, 319, 342, 516
NlaIII CATG 1 cut(s) 770
NlaIV GGNNCC 3 cut(s) 160, 421, 783
NmuCI GTSAC 2 cut(s) 671, 760
NsiI ATGCAT 1 cut(s) 775
PciSI GCTCTTC 1 cut(s) 472
PcsI WCGNNNNNNNCGW 2 cut(s) 32, 246
PfeI GAWTC 7 cut(s) 94, 212, 237, 442, 463, 503, 556
PkrI GCNGC 5 cut(s) 167, 484, 487, 578, 581
Ple19I CGATCG 1 cut(s) 53
PleI GAGTC 2 cut(s) 52, 119
PpsI GAGTC 2 cut(s) 52, 119
PpuMI RGGWCCY 1 cut(s) 158
Psp5II RGGWCCY 1 cut(s) 158
Psp6I CCWGG 1 cut(s) 739
PspEI GGTNACC 1 cut(s) 671
PspGI CCWGG 1 cut(s) 739
PspN4I GGNNCC 3 cut(s) 160, 421, 783
PspPI GGNCC 3 cut(s) 158, 690, 702
PspPPI RGGWCCY 1 cut(s) 158
PsuI RGATCY 1 cut(s) 319
PvuI CGATCG 1 cut(s) 53
RseI CAYNNNNRTG 1 cut(s) 662
SapI GCTCTTC 1 cut(s) 472
SaqAI TTAA 2 cut(s) 267, 360
SatI GCNGC 5 cut(s) 166, 483, 486, 577, 580
Sau3AI GATC 9 cut(s) 50, 120, 169, 188, 250, 288, 319, 342, 516
Sau96I GGNCC 3 cut(s) 158, 690, 702
SchI GAGTC 2 cut(s) 52, 119
ScrFI CCNGG 3 cut(s) 12, 162, 741
SduI GDGCHC 2 cut(s) 116, 682
SetI ASST 7 cut(s) 43, 160, 312, 549, 630, 673, 783
SfaNI GCATC 2 cut(s) 216, 760
SinI GGWCC 2 cut(s) 158, 702
SmiMI CAYNNNNRTG 1 cut(s) 662
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
Sse9I AATT 3 cut(s) 67, 203, 528
SsiI CCGC 7 cut(s) 165, 194, 576, 579, 582, 596, 683
StyD4I CCNGG 3 cut(s) 10, 160, 739
TaaI ACNGT 5 cut(s) 264, 401, 512, 702, 712
TasI AATT 3 cut(s) 67, 203, 528
TauI GCSGC 3 cut(s) 168, 579, 582
TfiI GAWTC 7 cut(s) 94, 212, 237, 442, 463, 503, 556
Tru1I TTAA 2 cut(s) 267, 360
Tru9I TTAA 2 cut(s) 267, 360
TseFI GTSAC 2 cut(s) 671, 760
TseI GCWGC 2 cut(s) 482, 485
Tsp45I GTSAC 2 cut(s) 671, 760
TspDTI ATGAA 2 cut(s) 638, 646
TspGWI ACGGA 2 cut(s) 165, 344
VneI GTGCAC 1 cut(s) 112
VpaK11BI GGWCC 2 cut(s) 158, 702
XapI RAATTY 1 cut(s) 203
Zsp2I ATGCAT 1 cut(s) 775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.