MD13G1039500.v1.1

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002115
Physical Location & Seq
Forward (+)
2696884 .. 2698811
1928 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1039500.v1.1.491

Sequence Viewer

Length: 1044 bp
ATGGTGTATATAGGCGGGTCGGAGGAGGAAGAGAGAGTGGCGGCGAAGTCCATGGCGATGGGTTCCGAGAGGACGAAGGCTCTGCACAATTTCAACTTGCCGTGGGATTTGAAGTGGGGGAACCAGAGGCACCTGCGGTGCCAGAAGGAGAGCTCCGAGGCCGGCGGGAGTGGTGGGGAAGGGTCGGAGGCTAATCGGCGATCGTCGGCTCAGAGGATGGAGAGCTCTCCGGCGGTGACTCGGCGGAGGGATTTGGAGTTCGAGAAGCGGAAGTTTCGGGCCCCGAGGCCGAGGATCGAAAACGACGACGCGGAGGAAGGGATCGATGCGTTTAGGCAGAAGCTCATGTTTGATCTCAAGACGGCGGCGGATAAGATGAAAGACGCGATTTTGAGGGAGGAGGAGGTGGCAGTGGCGGGGGCGGCTGCTTATACTGACGTGGCGGCGGCAGAGGAGGATGTTGAGATGGCGGAGGTTGGCGAATCGGAACCTCCGGTGGCGCCGGAGCCAGAGCCGCCGGAGGCGAGGCCGTGGAATCTGAGGACGAGGAGGGCGGCTTGCAAGGCTCCAATGGGCGGCGGCGGCGCTAAGGTGGTGAGAATCGAGGAAAAGAAGGCGAATTACTCGCCGTTGAGGAGTGAGGGTAACAATGGCGTGAAGTCGCCGAGGCTGGTGAGAGGGGCTTCGGTTTCGGGTCCAGAGAAGAATAAGGAGGAGGAGAGGCCCAAATTCTCGTTGACGCTCACCAAGAAGGAGATCGAGGAAGATTTCATGAAGATGCTCGGCCACCGCCCTCTCCGACGCCCAAAGAGGCGGCCAAGAAATGTTCAGAAGCAATTGGATGGTTTGTTTCCCGGTACGTGGTTGACGGAGGTCTCGGCGGAGACGTACAAGGTTCCTGAGTTCCCTGAAAATACAAAGGTAGCGATCCGGGCAAACGCGGGCTTATGTGGAAAGTTTCAAGGCTCTTGTTTATTTCTGACTGCTGCTGCTGCTGCTTGCAAAGAGGGTATGCCCATACACACATACACATTGGAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

348

Amino Acids

38.58

Weight (kDa)

8.67

Isoelectric Point (pI)

73.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1639 PF07797 248 - 297 1.1e-26 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 141
Acc36I ACCTGC 1 cut(s) 141
AccB1I GGYRCC 3 cut(s) 129, 138, 499
AccII CGCG 3 cut(s) 311, 386, 941
AclWI GGATC 3 cut(s) 302, 329, 922
AcoI YGGCCR 2 cut(s) 784, 815
AcsI RAATTY 1 cut(s) 728
AcyI GRCGYC 2 cut(s) 500, 802
AfaI GTAC 2 cut(s) 859, 890
AfiI CCNNNNNNNGG 2 cut(s) 575, 861
AgsI TTSAA 3 cut(s) 94, 112, 962
AjiI CACGTC 1 cut(s) 439
AloI GAACNNNNNNTCC 2 cut(s) 242, 274
AluBI AGCT 3 cut(s) 153, 225, 343
AluI AGCT 3 cut(s) 153, 225, 343
Alw21I GWGCWC 2 cut(s) 155, 227
Alw26I GTCTC 2 cut(s) 878, 880
AlwI GGATC 3 cut(s) 302, 329, 922
Ama87I CYCGRG 1 cut(s) 283
AoxI GGCC 7 cut(s) 159, 279, 287, 527, 722, 784, 815
ApaI GGGCCC 1 cut(s) 283
ApeKI GCWGC 5 cut(s) 425, 986, 989, 992, 995
ApoI RAATTY 1 cut(s) 728
AspLEI GCGC 2 cut(s) 502, 587
AspS9I GGNCC 4 cut(s) 279, 280, 695, 723
AsuC2I CCSGG 2 cut(s) 855, 932
AsuHPI GGTGA 4 cut(s) 247, 607, 685, 736
AvaI CYCGRG 1 cut(s) 283
AvaII GGWCC 1 cut(s) 695
BaeGI GKGCMC 1 cut(s) 283
BanI GGYRCC 3 cut(s) 129, 138, 499
BanII GRGCYC 3 cut(s) 155, 227, 283
BarI GAAGNNNNNNTAC 2 cut(s) 605, 637
Bbv12I GWGCWC 2 cut(s) 155, 227
BbvI GCAGC 5 cut(s) 412, 973, 976, 979, 982
BccI CCATC 4 cut(s) 52, 211, 460, 836
BceAI ACGGC 4 cut(s) 85, 378, 514, 613
BcgI CGANNNNNNTGC 2 cut(s) 64, 98
BcnI CCSGG 2 cut(s) 855, 932
BcoDI GTCTC 2 cut(s) 878, 880
BfoI RGCGCY 2 cut(s) 503, 588
BfuAI ACCTGC 1 cut(s) 141
Bme1390I CCNGG 2 cut(s) 855, 932
Bme18I GGWCC 1 cut(s) 695
BmeT110I CYCGRG 1 cut(s) 283
BmgBI CACGTC 1 cut(s) 439
BmgT120I GGNCC 4 cut(s) 279, 280, 695, 723
BmrFI CCNGG 2 cut(s) 855, 932
BmsI GCATC 2 cut(s) 316, 768
BoxI GACNNNNGTC 1 cut(s) 872
Bpu10I CCTNAGC 1 cut(s) 588
BpuEI CTTGAG 1 cut(s) 341
BpuMI CCSGG 2 cut(s) 855, 932
Bsa29I ATCGAT 1 cut(s) 324
BsaAI YACGTR 1 cut(s) 861
BsaHI GRCGYC 2 cut(s) 500, 802
BsaI GGTCTC 1 cut(s) 880
BsaJI CCNNGG 7 cut(s) 51, 101, 156, 284, 290, 530, 665
BsaWI WCCGGW 1 cut(s) 493
BsaXI ACNNNNNCTCC 2 cut(s) 628, 658
Bsc4I CCNNNNNNNGG 2 cut(s) 575, 861
Bse118I RCCGGY 1 cut(s) 161
BseCI ATCGAT 1 cut(s) 324
BseDI CCNNGG 7 cut(s) 51, 101, 156, 284, 290, 530, 665
BseGI GGATG 3 cut(s) 222, 463, 847
BseLI CCNNNNNNNGG 2 cut(s) 575, 861
BseMII CTCAG 3 cut(s) 224, 530, 891
BseRI GAGGAG 8 cut(s) 38, 413, 416, 467, 562, 649, 728, 731
BseSI GKGCMC 1 cut(s) 283
BseXI GCAGC 5 cut(s) 412, 973, 976, 979, 982
BsgI GTGCAG 1 cut(s) 68
Bsh1236I CGCG 3 cut(s) 311, 386, 941
Bsh1285I CGRYCG 1 cut(s) 203
BshFI GGCC 7 cut(s) 161, 281, 289, 529, 724, 786, 817
BshNI GGYRCC 3 cut(s) 129, 138, 499
BshVI ATCGAT 1 cut(s) 324
BsiEI CGRYCG 1 cut(s) 203
BsiHKAI GWGCWC 2 cut(s) 155, 227
BsiHKCI CYCGRG 1 cut(s) 283
BsiSI CCGG 7 cut(s) 162, 230, 494, 503, 518, 855, 931
BslI CCNNNNNNNGG 2 cut(s) 575, 861
BsmAI GTCTC 2 cut(s) 878, 880
BsmBI CGTCTC 1 cut(s) 878
BsnI GGCC 7 cut(s) 161, 281, 289, 529, 724, 786, 817
Bso31I GGTCTC 1 cut(s) 880
BsoBI CYCGRG 1 cut(s) 283
Bsp120I GGGCCC 1 cut(s) 279
Bsp1286I GDGCHC 3 cut(s) 155, 227, 283
Bsp143I GATC 6 cut(s) 200, 294, 321, 352, 756, 927
Bsp19I CCATGG 1 cut(s) 51
BspANI GGCC 7 cut(s) 161, 281, 289, 529, 724, 786, 817
BspCNI CTCAG 3 cut(s) 223, 531, 892
BspDI ATCGAT 1 cut(s) 324
BspFNI CGCG 3 cut(s) 311, 386, 941
BspHI TCATGA 1 cut(s) 771
BspMI ACCTGC 1 cut(s) 141
BspPI GGATC 3 cut(s) 302, 329, 922
BspT107I GGYRCC 3 cut(s) 129, 138, 499
BspTNI GGTCTC 1 cut(s) 880
BsrFI RCCGGY 1 cut(s) 161
BssAI RCCGGY 1 cut(s) 161
BssECI CCNNGG 7 cut(s) 51, 101, 156, 284, 290, 530, 665
BssMI GATC 6 cut(s) 200, 294, 321, 352, 756, 927
BssNI GRCGYC 2 cut(s) 500, 802
BssT1I CCWWGG 1 cut(s) 51
Bst6I CTCTTC 1 cut(s) 24
BstACI GRCGYC 2 cut(s) 500, 802
BstBAI YACGTR 1 cut(s) 861
BstC8I GCNNGC 4 cut(s) 163, 559, 943, 1000
BstDEI CTNAG 4 cut(s) 210, 539, 588, 900
BstDSI CCRYGG 3 cut(s) 51, 101, 530
BstF5I GGATG 3 cut(s) 222, 463, 847
BstFNI CGCG 3 cut(s) 311, 386, 941
BstH2I RGCGCY 2 cut(s) 503, 588
BstHHI GCGC 2 cut(s) 502, 587
BstKTI GATC 6 cut(s) 203, 297, 324, 355, 759, 930
BstMAI GTCTC 2 cut(s) 878, 880
BstMBI GATC 6 cut(s) 200, 294, 321, 352, 756, 927
BstMCI CGRYCG 1 cut(s) 203
BstMWI GCNNNNNNNGC 7 cut(s) 422, 514, 563, 582, 932, 992, 995
BstPAI GACNNNNGTC 1 cut(s) 872
BstSCI CCNGG 2 cut(s) 853, 930
BstSLI GKGCMC 1 cut(s) 283
BstUI CGCG 3 cut(s) 311, 386, 941
BstV1I GCAGC 5 cut(s) 412, 973, 976, 979, 982
BstXI CCANNNNNNTGG 1 cut(s) 58
Bsu15I ATCGAT 1 cut(s) 324
BsuRI GGCC 7 cut(s) 161, 281, 289, 529, 724, 786, 817
BsuTUI ATCGAT 1 cut(s) 324
BtgI CCRYGG 3 cut(s) 51, 101, 530
BtgZI GCGATG 1 cut(s) 71
BtrI CACGTC 1 cut(s) 439
BtsCI GGATG 3 cut(s) 222, 463, 847
BtsI GCAGTG 1 cut(s) 417
BtsIMutI CAGTG 1 cut(s) 417
BveI ACCTGC 1 cut(s) 141
Cac8I GCNNGC 4 cut(s) 163, 559, 943, 1000
CciI TCATGA 1 cut(s) 771
CfoI GCGC 2 cut(s) 502, 587
Cfr10I RCCGGY 1 cut(s) 161
Cfr13I GGNCC 4 cut(s) 279, 280, 695, 723
ClaI ATCGAT 1 cut(s) 324
CseI GACGC 4 cut(s) 317, 392, 748, 810
Csp6I GTAC 2 cut(s) 858, 889
CviAII CATG 3 cut(s) 52, 346, 772
CviQI GTAC 2 cut(s) 858, 889
DdeI CTNAG 4 cut(s) 210, 539, 588, 900
DinI GGCGCC 1 cut(s) 501
DpnI GATC 6 cut(s) 202, 296, 323, 354, 758, 929
DpnII GATC 6 cut(s) 200, 294, 321, 352, 756, 927
EaeI YGGCCR 2 cut(s) 784, 815
Eam1104I CTCTTC 1 cut(s) 24
EarI CTCTTC 1 cut(s) 24
EciI GGCGGA 4 cut(s) 259, 383, 485, 896
Ecl136II GAGCTC 2 cut(s) 153, 225
Eco130I CCWWGG 1 cut(s) 51
Eco24I GRGCYC 3 cut(s) 155, 227, 283
Eco31I GGTCTC 1 cut(s) 880
Eco47I GGWCC 1 cut(s) 695
Eco53kI GAGCTC 2 cut(s) 153, 225
Eco88I CYCGRG 1 cut(s) 283
EcoICRI GAGCTC 2 cut(s) 153, 225
EcoO109I RGGNCCY 1 cut(s) 280
EcoT14I CCWWGG 1 cut(s) 51
EcoT38I GRGCYC 3 cut(s) 155, 227, 283
EgeI GGCGCC 1 cut(s) 501
EheI GGCGCC 1 cut(s) 501
ErhI CCWWGG 1 cut(s) 51
Esp3I CGTCTC 1 cut(s) 878
FaeI CATG 3 cut(s) 55, 349, 775
FatI CATG 3 cut(s) 51, 345, 771
FauI CCCGC 4 cut(s) 8, 158, 409, 934
FokI GGATG 3 cut(s) 229, 470, 854
FriOI GRGCYC 3 cut(s) 155, 227, 283
GlaI GCGC 2 cut(s) 501, 586
HaeII RGCGCY 2 cut(s) 503, 588
HaeIII GGCC 7 cut(s) 161, 281, 289, 529, 724, 786, 817
HapII CCGG 7 cut(s) 162, 230, 494, 503, 518, 855, 931
HgaI GACGC 4 cut(s) 317, 392, 748, 810
HhaI GCGC 2 cut(s) 502, 587
Hin1I GRCGYC 2 cut(s) 500, 802
Hin1II CATG 3 cut(s) 55, 349, 775
Hin6I GCGC 2 cut(s) 500, 585
HinP1I GCGC 2 cut(s) 500, 585
HincII GTYRAC 2 cut(s) 738, 867
HindII GTYRAC 2 cut(s) 738, 867
HinfI GANTC 4 cut(s) 238, 482, 535, 600
HpaII CCGG 7 cut(s) 162, 230, 494, 503, 518, 855, 931
HphI GGTGA 4 cut(s) 247, 607, 685, 736
Hpy166II GTNNAC 2 cut(s) 738, 867
Hpy188III TCNNGA 5 cut(s) 262, 358, 698, 772, 899
Hpy8I GTNNAC 2 cut(s) 738, 867
Hpy99I CGWCG 4 cut(s) 208, 308, 311, 804
HpyAV CCTTC 6 cut(s) 70, 139, 173, 311, 607, 745
HpyCH4IV ACGT 3 cut(s) 438, 860, 887
HpyCH4V TGCA 3 cut(s) 85, 561, 1002
HpyF10VI GCNNNNNNNGC 7 cut(s) 422, 514, 563, 582, 932, 992, 995
HpyF3I CTNAG 4 cut(s) 210, 539, 588, 900
HpySE526I ACGT 3 cut(s) 438, 860, 887
Hsp92I GRCGYC 2 cut(s) 500, 802
Hsp92II CATG 3 cut(s) 55, 349, 775
HspAI GCGC 2 cut(s) 500, 585
KasI GGCGCC 1 cut(s) 499
KroI GCCGGC 1 cut(s) 161
KroNI GCCGGC 1 cut(s) 163
Kzo9I GATC 6 cut(s) 200, 294, 321, 352, 756, 927
LmnI GCTCC 3 cut(s) 158, 505, 571
Lsp1109I GCAGC 5 cut(s) 412, 973, 976, 979, 982
LweI GCATC 2 cut(s) 316, 768
MaeII ACGT 3 cut(s) 438, 860, 887
MaeIII GTNAC 2 cut(s) 235, 644
MalI GATC 6 cut(s) 202, 296, 323, 354, 758, 929
MboI GATC 6 cut(s) 200, 294, 321, 352, 756, 927
MboII GAAGA 4 cut(s) 41, 715, 776, 787
MfeI CAATTG 1 cut(s) 836
MhlI GDGCHC 3 cut(s) 155, 227, 283
MluCI AATT 4 cut(s) 88, 619, 728, 836
Mly113I GGCGCC 1 cut(s) 500
MlyI GAGTC 1 cut(s) 232
MmeI TCCRAC 2 cut(s) 165, 823
MroNI GCCGGC 1 cut(s) 161
MslI CAYNNNNRTG 2 cut(s) 56, 776
MspI CCGG 7 cut(s) 162, 230, 494, 503, 518, 855, 931
MspR9I CCNGG 2 cut(s) 855, 932
MunI CAATTG 1 cut(s) 836
MvnI CGCG 3 cut(s) 311, 386, 941
MwoI GCNNNNNNNGC 7 cut(s) 422, 514, 563, 582, 932, 992, 995
NaeI GCCGGC 1 cut(s) 163
NarI GGCGCC 1 cut(s) 500
NciI CCSGG 2 cut(s) 855, 932
NcoI CCATGG 1 cut(s) 51
NdeII GATC 6 cut(s) 200, 294, 321, 352, 756, 927
NgoMIV GCCGGC 1 cut(s) 161
NlaIII CATG 3 cut(s) 55, 349, 775
NmeAIII GCCGAG 5 cut(s) 220, 315, 690, 762, 857
NmuCI GTSAC 1 cut(s) 235
PagI TCATGA 1 cut(s) 771
PaqCI CACCTGC 1 cut(s) 141
PcsI WCGNNNNNNNCGW 2 cut(s) 303, 884
PdiI GCCGGC 1 cut(s) 163
PfeI GAWTC 3 cut(s) 482, 535, 600
Ple19I CGATCG 1 cut(s) 203
PleI GAGTC 1 cut(s) 232
PluTI GGCGCC 1 cut(s) 503
PpsI GAGTC 1 cut(s) 232
Ppu21I YACGTR 1 cut(s) 861
PshAI GACNNNNGTC 1 cut(s) 872
Psp124BI GAGCTC 2 cut(s) 155, 227
PspOMI GGGCCC 1 cut(s) 279
PspPI GGNCC 4 cut(s) 279, 280, 695, 723
PvuI CGATCG 1 cut(s) 203
RsaI GTAC 2 cut(s) 859, 890
RsaNI GTAC 2 cut(s) 858, 889
RseI CAYNNNNRTG 2 cut(s) 56, 776
SacI GAGCTC 2 cut(s) 155, 227
Sau3AI GATC 6 cut(s) 200, 294, 321, 352, 756, 927
Sau96I GGNCC 4 cut(s) 279, 280, 695, 723
SchI GAGTC 1 cut(s) 232
ScrFI CCNGG 2 cut(s) 855, 932
SduI GDGCHC 3 cut(s) 155, 227, 283
SfaNI GCATC 2 cut(s) 316, 768
SfoI GGCGCC 1 cut(s) 501
SinI GGWCC 1 cut(s) 695
SmiMI CAYNNNNRTG 2 cut(s) 56, 776
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
Sse9I AATT 4 cut(s) 88, 619, 728, 836
SspDI GGCGCC 1 cut(s) 499
SstI GAGCTC 2 cut(s) 155, 227
StyD4I CCNGG 2 cut(s) 853, 930
StyI CCWWGG 1 cut(s) 51
TaiI ACGT 3 cut(s) 441, 863, 890
TaqI TCGA 5 cut(s) 261, 297, 324, 603, 759
TasI AATT 4 cut(s) 88, 619, 728, 836
TfiI GAWTC 3 cut(s) 482, 535, 600
TscAI CASTG 1 cut(s) 417
TseFI GTSAC 1 cut(s) 235
TseI GCWGC 5 cut(s) 425, 986, 989, 992, 995
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 3 cut(s) 392, 760, 788
TspGWI ACGGA 1 cut(s) 884
TspRI CASTG 1 cut(s) 417
VpaK11BI GGWCC 1 cut(s) 695
XapI RAATTY 1 cut(s) 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.