Rorug04G0279500

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002115
Physical Location & Seq
Forward (+)
45238985 .. 45240429
1445 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0279500.1

Sequence Viewer

Length: 324 bp
ATGAGCCTAGTTGGGGGTGGGGATGCTATCCAGGACCCTTTTTTCTACTTGTGTGGTCGACTTTTGACCAAGAAACTGGTGGTGATCCTGTCATTCCCAGCACTGATCTCCAGTGTATGGGTATTGAAAGAAAGAGTTCTGATCCGACAAGAGGATGAAGGAATTTTCGTCTTCCAATTTAAGGATTCGGAGGTGAAGAATCGAGTCATTAATGGAGGACCTTGGTTCTATACCAACTCCATGCTGCTTCTAGCGGATTATGACGGCGTCAATGCTCTCGAAGAGGCGCCGCTGCATTTCCTCGGGGTGTGGGTGGTGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

107

Amino Acids

12.06

Weight (kDa)

4.96

Isoelectric Point (pI)

37.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 35 - 87 3.3e-08 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 286
AccB7I CCANNNNNTGG 1 cut(s) 117
AccI GTMKAC 1 cut(s) 58
AciI CCGC 2 cut(s) 254, 290
AclWI GGATC 2 cut(s) 79, 136
AcsI RAATTY 1 cut(s) 162
AcyI GRCGYC 2 cut(s) 267, 287
AfiI CCNNNNNNNGG 4 cut(s) 13, 117, 151, 181
AgsI TTSAA 1 cut(s) 127
AjnI CCWGG 1 cut(s) 30
AlwI GGATC 2 cut(s) 79, 136
Ama87I CYCGRG 1 cut(s) 302
ApeKI GCWGC 2 cut(s) 244, 292
ApoI RAATTY 1 cut(s) 162
AseI ATTAAT 1 cut(s) 210
Asp700I GAANNNNTTC 1 cut(s) 135
AspLEI GCGC 1 cut(s) 289
AspS9I GGNCC 2 cut(s) 34, 218
AsuHPI GGTGA 2 cut(s) 94, 205
AvaI CYCGRG 1 cut(s) 302
AvaII GGWCC 2 cut(s) 34, 218
BanI GGYRCC 1 cut(s) 286
BbsI GAAGAC 1 cut(s) 163
BbvI GCAGC 2 cut(s) 231, 279
BceAI ACGGC 1 cut(s) 280
BciT130I CCWGG 1 cut(s) 32
BfaI CTAG 3 cut(s) 8, 251, 322
BfoI RGCGCY 1 cut(s) 290
BisI GCNGC 3 cut(s) 245, 290, 293
BlsI GCNGC 3 cut(s) 246, 291, 294
Bme1390I CCNGG 1 cut(s) 32
Bme18I GGWCC 2 cut(s) 34, 218
BmeT110I CYCGRG 1 cut(s) 302
BmgT120I GGNCC 2 cut(s) 34, 218
BmiI GGNNCC 2 cut(s) 36, 288
BmrFI CCNGG 1 cut(s) 32
BmsI GCATC 1 cut(s) 13
BpiI GAAGAC 1 cut(s) 163
BpmI CTGGAG 1 cut(s) 94
BsaHI GRCGYC 2 cut(s) 267, 287
BsaJI CCNNGG 2 cut(s) 221, 301
Bsc4I CCNNNNNNNGG 4 cut(s) 13, 117, 151, 181
Bse1I ACTGG 2 cut(s) 81, 111
BseBI CCWGG 1 cut(s) 32
BseDI CCNNGG 2 cut(s) 221, 301
BseGI GGATG 2 cut(s) 28, 160
BseLI CCNNNNNNNGG 4 cut(s) 13, 117, 151, 181
BseNI ACTGG 2 cut(s) 81, 111
BseXI GCAGC 2 cut(s) 231, 279
BseYI CCCAGC 1 cut(s) 97
BshNI GGYRCC 1 cut(s) 286
BsiHKCI CYCGRG 1 cut(s) 302
BslI CCNNNNNNNGG 4 cut(s) 13, 117, 151, 181
BsoBI CYCGRG 1 cut(s) 302
Bsp143I GATC 3 cut(s) 84, 105, 141
BspACI CCGC 2 cut(s) 254, 290
BspLI GGNNCC 2 cut(s) 36, 288
BspPI GGATC 2 cut(s) 79, 136
BspT107I GGYRCC 1 cut(s) 286
BsrI ACTGG 2 cut(s) 81, 111
BssECI CCNNGG 2 cut(s) 221, 301
BssMI GATC 3 cut(s) 84, 105, 141
BssNI GRCGYC 2 cut(s) 267, 287
BssT1I CCWWGG 1 cut(s) 221
Bst2UI CCWGG 1 cut(s) 32
Bst6I CTCTTC 1 cut(s) 276
BstACI GRCGYC 2 cut(s) 267, 287
BstF5I GGATG 2 cut(s) 28, 160
BstH2I RGCGCY 1 cut(s) 290
BstHHI GCGC 1 cut(s) 289
BstKTI GATC 3 cut(s) 87, 108, 144
BstMBI GATC 3 cut(s) 84, 105, 141
BstNI CCWGG 1 cut(s) 32
BstSCI CCNGG 1 cut(s) 30
BstV1I GCAGC 2 cut(s) 231, 279
BstV2I GAAGAC 1 cut(s) 163
BstXI CCANNNNNNTGG 1 cut(s) 76
BtsCI GGATG 2 cut(s) 28, 160
BtsIMutI CAGTG 2 cut(s) 101, 118
CfoI GCGC 1 cut(s) 289
Cfr13I GGNCC 2 cut(s) 34, 218
CseI GACGC 1 cut(s) 256
CviAII CATG 1 cut(s) 241
CviJI RGCY 1 cut(s) 6
CviKI_1 RGCY 1 cut(s) 6
DinI GGCGCC 1 cut(s) 288
DpnI GATC 3 cut(s) 86, 107, 143
DpnII GATC 3 cut(s) 84, 105, 141
Eam1104I CTCTTC 1 cut(s) 276
EarI CTCTTC 1 cut(s) 276
Eco130I CCWWGG 1 cut(s) 221
Eco47I GGWCC 2 cut(s) 34, 218
Eco88I CYCGRG 1 cut(s) 302
EcoO109I RGGNCCY 2 cut(s) 34, 218
EcoRII CCWGG 1 cut(s) 30
EcoT14I CCWWGG 1 cut(s) 221
EgeI GGCGCC 1 cut(s) 288
EheI GGCGCC 1 cut(s) 288
ErhI CCWWGG 1 cut(s) 221
FaeI CATG 1 cut(s) 244
FaiI YATR 4 cut(s) 118, 231, 242, 261
FatI CATG 1 cut(s) 240
FblI GTMKAC 1 cut(s) 58
Fnu4HI GCNGC 3 cut(s) 245, 290, 293
FokI GGATG 2 cut(s) 35, 167
Fsp4HI GCNGC 3 cut(s) 245, 290, 293
FspBI CTAG 3 cut(s) 8, 251, 322
GlaI GCGC 1 cut(s) 288
GluI GCNGC 3 cut(s) 245, 290, 293
GsaI CCCAGC 1 cut(s) 101
GsuI CTGGAG 1 cut(s) 94
HaeII RGCGCY 1 cut(s) 290
HgaI GACGC 1 cut(s) 256
HhaI GCGC 1 cut(s) 289
Hin1I GRCGYC 2 cut(s) 267, 287
Hin1II CATG 1 cut(s) 244
Hin6I GCGC 1 cut(s) 287
HinP1I GCGC 1 cut(s) 287
HincII GTYRAC 1 cut(s) 59
HindII GTYRAC 1 cut(s) 59
HinfI GANTC 3 cut(s) 185, 199, 204
HphI GGTGA 2 cut(s) 94, 205
Hpy166II GTNNAC 1 cut(s) 59
Hpy188I TCNGA 3 cut(s) 141, 146, 190
Hpy188III TCNNGA 1 cut(s) 278
Hpy8I GTNNAC 1 cut(s) 59
HpyAV CCTTC 1 cut(s) 152
HpyCH4V TGCA 1 cut(s) 295
Hsp92I GRCGYC 2 cut(s) 267, 287
Hsp92II CATG 1 cut(s) 244
HspAI GCGC 1 cut(s) 287
KasI GGCGCC 1 cut(s) 286
Kzo9I GATC 3 cut(s) 84, 105, 141
LpnPI CCDG 6 cut(s) 17, 44, 62, 101, 111, 124
Lsp1109I GCAGC 2 cut(s) 231, 279
LweI GCATC 1 cut(s) 13
MaeI CTAG 3 cut(s) 8, 251, 322
MalI GATC 3 cut(s) 86, 107, 143
MboI GATC 3 cut(s) 84, 105, 141
MboII GAAGA 3 cut(s) 163, 208, 293
MluCI AATT 2 cut(s) 162, 176
Mly113I GGCGCC 1 cut(s) 287
MlyI GAGTC 1 cut(s) 213
MmeI TCCRAC 1 cut(s) 169
MnlI CCTC 5 cut(s) 145, 184, 209, 277, 311
MroXI GAANNNNTTC 1 cut(s) 135
MseI TTAA 2 cut(s) 180, 210
MspA1I CMGCKG 1 cut(s) 292
MspR9I CCNGG 1 cut(s) 32
MvaI CCWGG 1 cut(s) 32
NarI GGCGCC 1 cut(s) 287
NdeII GATC 3 cut(s) 84, 105, 141
NlaIII CATG 1 cut(s) 244
NlaIV GGNNCC 2 cut(s) 36, 288
PdmI GAANNNNTTC 1 cut(s) 135
PfeI GAWTC 2 cut(s) 185, 199
PflFI GACNNNGTC 1 cut(s) 266
PflMI CCANNNNNTGG 1 cut(s) 117
PfoI TCCNGGA 1 cut(s) 30
PkrI GCNGC 3 cut(s) 246, 291, 294
PleI GAGTC 1 cut(s) 212
PluTI GGCGCC 1 cut(s) 290
PpsI GAGTC 1 cut(s) 212
PpuMI RGGWCCY 2 cut(s) 34, 218
PshBI ATTAAT 1 cut(s) 210
Psp5II RGGWCCY 2 cut(s) 34, 218
Psp6I CCWGG 1 cut(s) 30
PspFI CCCAGC 1 cut(s) 97
PspGI CCWGG 1 cut(s) 30
PspN4I GGNNCC 2 cut(s) 36, 288
PspPI GGNCC 2 cut(s) 34, 218
PspPPI RGGWCCY 2 cut(s) 34, 218
PsyI GACNNNGTC 1 cut(s) 266
SalI GTCGAC 1 cut(s) 57
SaqAI TTAA 2 cut(s) 180, 210
SatI GCNGC 3 cut(s) 245, 290, 293
Sau3AI GATC 3 cut(s) 84, 105, 141
Sau96I GGNCC 2 cut(s) 34, 218
SchI GAGTC 1 cut(s) 213
ScrFI CCNGG 1 cut(s) 32
SetI ASST 2 cut(s) 195, 223
SfaNI GCATC 1 cut(s) 13
SfoI GGCGCC 1 cut(s) 288
SinI GGWCC 2 cut(s) 34, 218
Sse9I AATT 2 cut(s) 162, 176
SsiI CCGC 2 cut(s) 254, 290
SspDI GGCGCC 1 cut(s) 286
SspMI CTAG 3 cut(s) 8, 251, 322
StyD4I CCNGG 1 cut(s) 30
StyI CCWWGG 1 cut(s) 221
TaqI TCGA 3 cut(s) 58, 202, 279
TasI AATT 2 cut(s) 162, 176
TauI GCSGC 1 cut(s) 292
TfiI GAWTC 2 cut(s) 185, 199
Tru1I TTAA 2 cut(s) 180, 210
Tru9I TTAA 2 cut(s) 180, 210
TscAI CASTG 2 cut(s) 108, 118
TseI GCWGC 2 cut(s) 244, 292
TspDTI ATGAA 1 cut(s) 171
TspRI CASTG 2 cut(s) 108, 118
Tth111I GACNNNGTC 1 cut(s) 266
Van91I CCANNNNNTGG 1 cut(s) 117
VpaK11BI GGWCC 2 cut(s) 34, 218
VspI ATTAAT 1 cut(s) 210
XapI RAATTY 1 cut(s) 162
XcmI CCANNNNNNNNNTGG 1 cut(s) 76
XmiI GTMKAC 1 cut(s) 58
XmnI GAANNNNTTC 1 cut(s) 135
XspI CTAG 3 cut(s) 8, 251, 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.