MD16G1040400.v1.1

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002115
Physical Location & Seq
Forward (+)
2868560 .. 2870481
1922 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1040400.v1.1.491

Sequence Viewer

Length: 939 bp
ATGGTGTATATAGGTGGGTCGGAGGAGGAGGAGAGAGTGGAGGCGAAGTCCATGGCGATGGGTTCTGAGAGGACGAAGCCTCTGCACAATTTCAACTTGCCGTGGGATTTGAAGTGGGGGAACCAGAAGTACCTGCGGTGCCAGAAGGAGAGCTCCGACGCCGGCGGGAGTGGCGGGGAAGGGTCTGGGGTTAATCGGCGATCGTCGGCTCAGAGGATTGAGAGCTCGCCGGCGGTTATGTCTCGGCGGAGGGATTTGGAGTTCGAGAAGCGCACGTTTCGGCCCTCGAGGCCGAGGATCGAGAACGACGACGCGGAGGAAGGGATCGATGCGTTTAGGCAGAAGCTCATGTTTGATCTCAAGACGGCGGCGGATAAGATGAAAGACGAGATTTTGAGGGAGGAGGAGGTGGCAGTGGCGGGGGCGGCTGCTGATACTCACGTGGCGGCGGAAGAGGAGGATGTTGAGATGGCGGAGGTTGGTGAATCGGAACCTCCGGTGGCGCCGGAGCCACCGGAGGCGAGGCCATGGAATCTGAGGACGAGAAGGGCGGCGTGCAAGTCTCCAATCGGCGGCGGTGGTGCTGCCAAGGGGGTGAGAATCGAGGAGAAGAAGGCGAATTACTCGCCGTTGAGGAGTGAGGGTAACAATGGCGTGAAGTCGCCAAGGCTAGTCAGAGGGGCTTCGGGTTCGGGTTCGGGTTCGGGTCCGGAGAAGAATAAGGAGGAGGAGAGGCCCAAGTTCTCATTGACGCTCACCAAGAAGGAGATCGAGGAAGATTTCATGAAGATGCTCGGCCACCGCCCTCCCCGTCGCCCAAAGAGGCGGCCAAGAAATGTTCAGAAGCAATTGGAAGGTTTGTTTCCCGGTACGTGGTTGACGGAGGTATCGGCGGAGACGTACAAGGTTCCTGAGGTCCCTGAAAATACAAAGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

313

Amino Acids

34.77

Weight (kDa)

8.37

Isoelectric Point (pI)

82.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1639 PF07797 252 - 301 3.9e-27 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 286
Acc36I ACCTGC 1 cut(s) 141
AccB1I GGYRCC 2 cut(s) 138, 502
AccII CGCG 1 cut(s) 314
AccIII TCCGGA 1 cut(s) 709
AclWI GGATC 2 cut(s) 305, 332
AcoI YGGCCR 2 cut(s) 796, 827
AcvI CACGTG 1 cut(s) 442
AcyI GRCGYC 2 cut(s) 159, 503
AfaI GTAC 3 cut(s) 131, 871, 902
AfiI CCNNNNNNNGG 2 cut(s) 572, 873
AgsI TTSAA 2 cut(s) 94, 112
AloI GAACNNNNNNTCC 2 cut(s) 245, 277
AluBI AGCT 3 cut(s) 153, 225, 346
AluI AGCT 3 cut(s) 153, 225, 346
Alw21I GWGCWC 2 cut(s) 155, 227
Alw26I GTCTC 3 cut(s) 246, 567, 890
AlwI GGATC 2 cut(s) 305, 332
Ama87I CYCGRG 1 cut(s) 286
Aor13HI TCCGGA 1 cut(s) 709
AoxI GGCC 6 cut(s) 281, 290, 524, 734, 796, 827
ApeKI GCWGC 2 cut(s) 428, 584
AspLEI GCGC 2 cut(s) 273, 505
AspS9I GGNCC 4 cut(s) 282, 707, 735, 916
AsuC2I CCSGG 1 cut(s) 867
AsuHPI GGTGA 3 cut(s) 494, 607, 748
AvaI CYCGRG 1 cut(s) 286
AvaII GGWCC 2 cut(s) 707, 916
AxyI CCTNAGG 1 cut(s) 912
BaeI ACNNNNGTAYC 4 cut(s) 426, 459, 870, 903
BanI GGYRCC 2 cut(s) 138, 502
BanII GRGCYC 2 cut(s) 155, 227
BarI GAAGNNNNNNTAC 2 cut(s) 605, 637
BbrPI CACGTG 1 cut(s) 442
Bbv12I GWGCWC 2 cut(s) 155, 227
BbvI GCAGC 2 cut(s) 415, 571
BccI CCATC 2 cut(s) 52, 463
BceAI ACGGC 3 cut(s) 85, 381, 613
BcgI CGANNNNNNTGC 2 cut(s) 64, 98
BcnI CCSGG 1 cut(s) 867
BcoDI GTCTC 3 cut(s) 246, 567, 890
BfaI CTAG 1 cut(s) 671
BfoI RGCGCY 1 cut(s) 506
BfuAI ACCTGC 1 cut(s) 141
BglI GCCNNNNNGGC 1 cut(s) 289
BisI GCNGC 8 cut(s) 369, 426, 429, 447, 552, 574, 585, 827
BlsI GCNGC 8 cut(s) 370, 427, 430, 448, 553, 575, 586, 828
Bme1390I CCNGG 1 cut(s) 867
Bme18I GGWCC 2 cut(s) 707, 916
BmeT110I CYCGRG 1 cut(s) 286
BmgT120I GGNCC 4 cut(s) 282, 707, 735, 916
BmiI GGNNCC 8 cut(s) 122, 140, 492, 504, 510, 708, 909, 918
BmrFI CCNGG 1 cut(s) 867
BmsI GCATC 2 cut(s) 319, 780
BpuEI CTTGAG 1 cut(s) 344
BpuMI CCSGG 1 cut(s) 867
Bsa29I ATCGAT 1 cut(s) 327
BsaAI YACGTR 2 cut(s) 442, 873
BsaHI GRCGYC 2 cut(s) 159, 503
BsaJI CCNNGG 6 cut(s) 51, 101, 293, 527, 588, 665
BsaWI WCCGGW 3 cut(s) 496, 514, 709
BsaXI ACNNNNNCTCC 4 cut(s) 32, 62, 628, 658
Bsc4I CCNNNNNNNGG 2 cut(s) 572, 873
Bse118I RCCGGY 2 cut(s) 161, 229
Bse21I CCTNAGG 1 cut(s) 912
BseAI TCCGGA 1 cut(s) 709
BseCI ATCGAT 1 cut(s) 327
BseDI CCNNGG 6 cut(s) 51, 101, 293, 527, 588, 665
BseGI GGATG 1 cut(s) 466
BseLI CCNNNNNNNGG 2 cut(s) 572, 873
BseMII CTCAG 4 cut(s) 57, 224, 527, 903
BseXI GCAGC 2 cut(s) 415, 571
BsgI GTGCAG 1 cut(s) 68
Bsh1236I CGCG 1 cut(s) 314
Bsh1285I CGRYCG 1 cut(s) 203
BshFI GGCC 6 cut(s) 283, 292, 526, 736, 798, 829
BshNI GGYRCC 2 cut(s) 138, 502
BshVI ATCGAT 1 cut(s) 327
BsiEI CGRYCG 1 cut(s) 203
BsiHKAI GWGCWC 2 cut(s) 155, 227
BsiHKCI CYCGRG 1 cut(s) 286
BsiSI CCGG 7 cut(s) 162, 230, 497, 506, 515, 710, 867
BslFI GGGAC 1 cut(s) 902
BslI CCNNNNNNNGG 2 cut(s) 572, 873
BsmAI GTCTC 3 cut(s) 246, 567, 890
BsmBI CGTCTC 1 cut(s) 890
BsmFI GGGAC 1 cut(s) 902
BsnI GGCC 6 cut(s) 283, 292, 526, 736, 798, 829
BsoBI CYCGRG 1 cut(s) 286
Bsp1286I GDGCHC 2 cut(s) 155, 227
Bsp13I TCCGGA 1 cut(s) 709
Bsp143I GATC 5 cut(s) 200, 297, 324, 355, 768
Bsp19I CCATGG 2 cut(s) 51, 527
BspANI GGCC 6 cut(s) 283, 292, 526, 736, 798, 829
BspCNI CTCAG 4 cut(s) 58, 223, 528, 904
BspDI ATCGAT 1 cut(s) 327
BspEI TCCGGA 1 cut(s) 709
BspFNI CGCG 1 cut(s) 314
BspHI TCATGA 1 cut(s) 783
BspLI GGNNCC 8 cut(s) 122, 140, 492, 504, 510, 708, 909, 918
BspMI ACCTGC 1 cut(s) 141
BspPI GGATC 2 cut(s) 305, 332
BspT107I GGYRCC 2 cut(s) 138, 502
BsrFI RCCGGY 2 cut(s) 161, 229
BssAI RCCGGY 2 cut(s) 161, 229
BssECI CCNNGG 6 cut(s) 51, 101, 293, 527, 588, 665
BssMI GATC 5 cut(s) 200, 297, 324, 355, 768
BssNI GRCGYC 2 cut(s) 159, 503
BssT1I CCWWGG 4 cut(s) 51, 527, 588, 665
Bst6I CTCTTC 1 cut(s) 447
BstACI GRCGYC 2 cut(s) 159, 503
BstBAI YACGTR 2 cut(s) 442, 873
BstC8I GCNNGC 4 cut(s) 163, 227, 231, 556
BstDEI CTNAG 4 cut(s) 66, 210, 536, 912
BstDSI CCRYGG 3 cut(s) 51, 101, 527
BstF5I GGATG 1 cut(s) 466
BstFNI CGCG 1 cut(s) 314
BstH2I RGCGCY 1 cut(s) 506
BstHHI GCGC 2 cut(s) 273, 505
BstKTI GATC 5 cut(s) 203, 300, 327, 358, 771
BstMAI GTCTC 3 cut(s) 246, 567, 890
BstMBI GATC 5 cut(s) 200, 297, 324, 355, 768
BstMCI CGRYCG 1 cut(s) 203
BstMWI GCNNNNNNNGC 3 cut(s) 171, 289, 425
BstSCI CCNGG 1 cut(s) 865
BstUI CGCG 1 cut(s) 314
BstV1I GCAGC 2 cut(s) 415, 571
BstXI CCANNNNNNTGG 1 cut(s) 58
Bsu15I ATCGAT 1 cut(s) 327
Bsu36I CCTNAGG 1 cut(s) 912
BsuRI GGCC 6 cut(s) 283, 292, 526, 736, 798, 829
BsuTUI ATCGAT 1 cut(s) 327
BtgI CCRYGG 3 cut(s) 51, 101, 527
BtgZI GCGATG 1 cut(s) 71
BtsCI GGATG 1 cut(s) 466
BtsI GCAGTG 1 cut(s) 420
BtsIMutI CAGTG 1 cut(s) 420
BveI ACCTGC 1 cut(s) 141
Cac8I GCNNGC 4 cut(s) 163, 227, 231, 556
CciI TCATGA 1 cut(s) 783
CfoI GCGC 2 cut(s) 273, 505
Cfr10I RCCGGY 2 cut(s) 161, 229
Cfr13I GGNCC 4 cut(s) 282, 707, 735, 916
ClaI ATCGAT 1 cut(s) 327
CseI GACGC 3 cut(s) 167, 320, 760
Csp6I GTAC 3 cut(s) 130, 870, 901
CviAII CATG 4 cut(s) 52, 349, 528, 784
CviQI GTAC 3 cut(s) 130, 870, 901
DdeI CTNAG 4 cut(s) 66, 210, 536, 912
DinI GGCGCC 1 cut(s) 504
DpnI GATC 5 cut(s) 202, 299, 326, 357, 770
DpnII GATC 5 cut(s) 200, 297, 324, 355, 768
EaeI YGGCCR 2 cut(s) 796, 827
Eam1104I CTCTTC 1 cut(s) 447
EarI CTCTTC 1 cut(s) 447
EciI GGCGGA 5 cut(s) 262, 386, 464, 488, 908
Ecl136II GAGCTC 2 cut(s) 153, 225
Eco130I CCWWGG 4 cut(s) 51, 527, 588, 665
Eco24I GRGCYC 2 cut(s) 155, 227
Eco47I GGWCC 2 cut(s) 707, 916
Eco53kI GAGCTC 2 cut(s) 153, 225
Eco72I CACGTG 1 cut(s) 442
Eco81I CCTNAGG 1 cut(s) 912
Eco88I CYCGRG 1 cut(s) 286
EcoICRI GAGCTC 2 cut(s) 153, 225
EcoO109I RGGNCCY 1 cut(s) 916
EcoT14I CCWWGG 4 cut(s) 51, 527, 588, 665
EcoT38I GRGCYC 2 cut(s) 155, 227
EgeI GGCGCC 1 cut(s) 504
EheI GGCGCC 1 cut(s) 504
ErhI CCWWGG 4 cut(s) 51, 527, 588, 665
Esp3I CGTCTC 1 cut(s) 890
FaeI CATG 4 cut(s) 55, 352, 531, 787
FaiI YATR 7 cut(s) 9, 11, 53, 239, 350, 529, 785
FaqI GGGAC 1 cut(s) 902
FatI CATG 4 cut(s) 51, 348, 527, 783
FauI CCCGC 3 cut(s) 158, 167, 412
Fnu4HI GCNGC 8 cut(s) 369, 426, 429, 447, 552, 574, 585, 827
FokI GGATG 1 cut(s) 473
FriOI GRGCYC 2 cut(s) 155, 227
Fsp4HI GCNGC 8 cut(s) 369, 426, 429, 447, 552, 574, 585, 827
FspBI CTAG 1 cut(s) 671
GlaI GCGC 2 cut(s) 272, 504
GluI GCNGC 8 cut(s) 369, 426, 429, 447, 552, 574, 585, 827
HaeII RGCGCY 1 cut(s) 506
HaeIII GGCC 6 cut(s) 283, 292, 526, 736, 798, 829
HapII CCGG 7 cut(s) 162, 230, 497, 506, 515, 710, 867
HgaI GACGC 3 cut(s) 167, 320, 760
HhaI GCGC 2 cut(s) 273, 505
Hin1I GRCGYC 2 cut(s) 159, 503
Hin1II CATG 4 cut(s) 55, 352, 531, 787
Hin6I GCGC 2 cut(s) 271, 503
HinP1I GCGC 2 cut(s) 271, 503
HincII GTYRAC 1 cut(s) 879
HindII GTYRAC 1 cut(s) 879
HinfI GANTC 3 cut(s) 485, 532, 600
HpaII CCGG 7 cut(s) 162, 230, 497, 506, 515, 710, 867
HphI GGTGA 3 cut(s) 494, 607, 748
Hpy166II GTNNAC 1 cut(s) 879
Hpy188I TCNGA 8 cut(s) 22, 67, 157, 213, 490, 537, 677, 843
Hpy188III TCNNGA 6 cut(s) 265, 301, 361, 710, 784, 911
Hpy8I GTNNAC 1 cut(s) 879
Hpy99I CGWCG 5 cut(s) 161, 208, 311, 314, 816
HpyAV CCTTC 7 cut(s) 139, 173, 314, 540, 607, 757, 848
HpyCH4IV ACGT 4 cut(s) 275, 441, 872, 899
HpyCH4V TGCA 2 cut(s) 85, 558
HpyF10VI GCNNNNNNNGC 3 cut(s) 171, 289, 425
HpyF3I CTNAG 4 cut(s) 66, 210, 536, 912
HpySE526I ACGT 4 cut(s) 275, 441, 872, 899
Hsp92I GRCGYC 2 cut(s) 159, 503
Hsp92II CATG 4 cut(s) 55, 352, 531, 787
HspAI GCGC 2 cut(s) 271, 503
KasI GGCGCC 1 cut(s) 502
Kpn2I TCCGGA 1 cut(s) 709
KroI GCCGGC 2 cut(s) 161, 229
KroNI GCCGGC 2 cut(s) 163, 231
Kzo9I GATC 5 cut(s) 200, 297, 324, 355, 768
LmnI GCTCC 2 cut(s) 158, 508
Lsp1109I GCAGC 2 cut(s) 415, 571
LweI GCATC 2 cut(s) 319, 780
MaeI CTAG 1 cut(s) 671
MaeII ACGT 4 cut(s) 275, 441, 872, 899
MaeIII GTNAC 1 cut(s) 644
MalI GATC 5 cut(s) 202, 299, 326, 357, 770
MboI GATC 5 cut(s) 200, 297, 324, 355, 768
MboII GAAGA 5 cut(s) 464, 622, 727, 788, 799
MfeI CAATTG 1 cut(s) 848
MhlI GDGCHC 2 cut(s) 155, 227
MluCI AATT 3 cut(s) 88, 619, 848
Mly113I GGCGCC 1 cut(s) 503
MmeI TCCRAC 1 cut(s) 180
MreI CGCCGGCG 2 cut(s) 161, 229
MroI TCCGGA 1 cut(s) 709
MroNI GCCGGC 2 cut(s) 161, 229
MseI TTAA 1 cut(s) 192
MslI CAYNNNNRTG 2 cut(s) 56, 788
MspI CCGG 7 cut(s) 162, 230, 497, 506, 515, 710, 867
MspR9I CCNGG 1 cut(s) 867
MunI CAATTG 1 cut(s) 848
MvnI CGCG 1 cut(s) 314
MwoI GCNNNNNNNGC 3 cut(s) 171, 289, 425
NaeI GCCGGC 2 cut(s) 163, 231
NarI GGCGCC 1 cut(s) 503
NciI CCSGG 1 cut(s) 867
NcoI CCATGG 2 cut(s) 51, 527
NdeII GATC 5 cut(s) 200, 297, 324, 355, 768
NgoMIV GCCGGC 2 cut(s) 161, 229
NlaIII CATG 4 cut(s) 55, 352, 531, 787
NlaIV GGNNCC 8 cut(s) 122, 140, 492, 504, 510, 708, 909, 918
NmeAIII GCCGAG 3 cut(s) 223, 318, 774
PaeR7I CTCGAG 1 cut(s) 286
PagI TCATGA 1 cut(s) 783
PcsI WCGNNNNNNNCGW 2 cut(s) 306, 896
PdiI GCCGGC 2 cut(s) 163, 231
PfeI GAWTC 3 cut(s) 485, 532, 600
PkrI GCNGC 8 cut(s) 370, 427, 430, 448, 553, 575, 586, 828
Ple19I CGATCG 1 cut(s) 203
PluTI GGCGCC 1 cut(s) 506
PmaCI CACGTG 1 cut(s) 442
PmlI CACGTG 1 cut(s) 442
Ppu21I YACGTR 2 cut(s) 442, 873
PpuMI RGGWCCY 1 cut(s) 916
Psp124BI GAGCTC 2 cut(s) 155, 227
Psp5II RGGWCCY 1 cut(s) 916
PspCI CACGTG 1 cut(s) 442
PspN4I GGNNCC 8 cut(s) 122, 140, 492, 504, 510, 708, 909, 918
PspPI GGNCC 4 cut(s) 282, 707, 735, 916
PspPPI RGGWCCY 1 cut(s) 916
PspXI VCTCGAGB 1 cut(s) 286
PsrI GAACNNNNNNTAC 2 cut(s) 113, 145
PvuI CGATCG 1 cut(s) 203
RsaI GTAC 3 cut(s) 131, 871, 902
RsaNI GTAC 3 cut(s) 130, 870, 901
RseI CAYNNNNRTG 2 cut(s) 56, 788
SacI GAGCTC 2 cut(s) 155, 227
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 8 cut(s) 369, 426, 429, 447, 552, 574, 585, 827
Sau3AI GATC 5 cut(s) 200, 297, 324, 355, 768
Sau96I GGNCC 4 cut(s) 282, 707, 735, 916
ScrFI CCNGG 1 cut(s) 867
SduI GDGCHC 2 cut(s) 155, 227
SfaNI GCATC 2 cut(s) 319, 780
SfiI GGCCNNNNNGGCC 1 cut(s) 289
SfoI GGCGCC 1 cut(s) 504
Sfr274I CTCGAG 1 cut(s) 286
SgrAI CRCCGGYG 2 cut(s) 161, 229
SinI GGWCC 2 cut(s) 707, 916
SlaI CTCGAG 1 cut(s) 286
SmiMI CAYNNNNRTG 2 cut(s) 56, 788
SmlI CTYRAG 2 cut(s) 286, 359
SmoI CTYRAG 2 cut(s) 286, 359
Sse9I AATT 3 cut(s) 88, 619, 848
SspDI GGCGCC 1 cut(s) 502
SspMI CTAG 1 cut(s) 671
SstI GAGCTC 2 cut(s) 155, 227
StyD4I CCNGG 1 cut(s) 865
StyI CCWWGG 4 cut(s) 51, 527, 588, 665
TaiI ACGT 4 cut(s) 278, 444, 875, 902
TaqI TCGA 6 cut(s) 264, 287, 300, 327, 603, 771
TasI AATT 3 cut(s) 88, 619, 848
TauI GCSGC 6 cut(s) 371, 428, 449, 554, 576, 829
TfiI GAWTC 3 cut(s) 485, 532, 600
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TscAI CASTG 1 cut(s) 420
TseI GCWGC 2 cut(s) 428, 584
TspDTI ATGAA 3 cut(s) 395, 772, 800
TspGWI ACGGA 1 cut(s) 896
TspRI CASTG 1 cut(s) 420
VpaK11BI GGWCC 2 cut(s) 707, 916
XhoI CTCGAG 1 cut(s) 286
XspI CTAG 1 cut(s) 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.