AT1G28580

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001486
Physical Location & Seq
Reverse (-)
10044337 .. 10046552
2216 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G28580.2

Sequence Viewer

Length: 1173 bp
ATGGCGTATCCCGGTTCGCCTATCTTGATGAAGCTCCTCGTCTTCATCTTTTTATCAACTTTTGTAGTCACTAACGTGAGCTCGGAAACTAAGTGCCGGGAGTTCAAATCGATCATCAGTTTCGGAGATTCCATTGCCGACACTGGAAACTTGCTTGGCCTCTCAGATCCTAAAGATCTTCCTCACATGGCGTTTCCACCGTATGGAGAGAACTTCTTCCACCACCCAACTGGTCGTTTCTCCAACGGCCGCCTCATCATTGATTTCATCGCTGAATTCTTGGGTTTACCGCTTGTGCCTCCATTCTATGGATCTCATAATGCAAACTTTGAAAAGGGAGTTAATTTCGCGGTAGGGGGAGCAACCGCTTTGGAACGTTCCTTTCTTGAAGACAGAGGAATTCATTTTCCTTACACTAATGTGAGTTTAGGCGTTCAGCTTAATAGCTTCAAGGAGAGTTTACCTAGCATATGTGGCTCTCCTTCAGACTGTAGAGATATGATAGAAAATGCTTTGATTCTCATGGGGGAAATTGGAGGGAATGACTATAACTACGCGTTCTTCGTGGACAAAGGCATTGAAGAGATCAAGGAGCTGATGCCCCTGGTGATCACTACTATTTCTTCTGCAATCACGGAGTTGATCGGTATGGGGGGAAGAACATTTTTGGTGCCCGGAGAGTTCCCAGTCGGATGCTCAGTATTGTATTTGACATCACATCAAACATCAAACATGGAAGAATACGATCCTTTAACAGGATGTTTGAAATGGCTGAACAAGTTTGGTGAAAACCATGGTGAGCAGCTTAGAGCAGAACTCAACAGACTCCAGAAGTTGTACCCTCATGTTAACATCATATACGCTGACTACTACAACGCGCTGTTTCACCTTTATCAAGAACCAGCCAAATTCGGATTCATGAACAGACCTTTGTCCGCTTGTTGCGGCGCAGGAGGACCGTACAACTATACCGTAGGTAGGAAATGTGGGACTGATATAGTTGAAAGTTGTGATGATCCTTCAAAGTATGTGGCTTGGGACGGTGTTCATATGACTGAGGCTGCGTACAGATTGATGGCTGAGGGTATACTAAATGGACCGTATGCGATTCCTCCTTTTGATTGGTCTTGCCGTAGCTCTGGAGTTAAGAACAGTGGGTCATCTGACACATAG

Protein Analysis

390

Amino Acids

43.02

Weight (kDa)

5.15

Isoelectric Point (pI)

40.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 11 - 282 5.8e-22 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 670
AccB7I CCANNNNNTGG 2 cut(s) 203, 308
AccI GTMKAC 1 cut(s) 1087
AccII CGCG 3 cut(s) 350, 557, 878
AciI CCGC 6 cut(s) 250, 290, 350, 366, 936, 945
AclI AACGTT 1 cut(s) 376
AclWI GGATC 4 cut(s) 161, 319, 740, 1010
AcoI YGGCCR 1 cut(s) 247
AcsI RAATTY 3 cut(s) 275, 399, 908
AcuI CTGAAG 1 cut(s) 468
AfaI GTAC 3 cut(s) 839, 962, 1067
AfiI CCNNNNNNNGG 4 cut(s) 203, 308, 755, 978
AflIII ACRYGT 1 cut(s) 555
AgsI TTSAA 8 cut(s) 106, 332, 389, 451, 581, 766, 1004, 1023
AjnI CCWGG 1 cut(s) 603
AjuI GAANNNNNNNTTGG 2 cut(s) 899, 931
AleI CACNNNNGTG 2 cut(s) 74, 419
AluBI AGCT 7 cut(s) 34, 81, 439, 447, 595, 805, 1137
AluI AGCT 7 cut(s) 34, 81, 439, 447, 595, 805, 1137
Alw21I GWGCWC 1 cut(s) 83
AlwI GGATC 4 cut(s) 161, 319, 740, 1010
AoxI GGCC 2 cut(s) 157, 247
ApeKI GCWGC 2 cut(s) 802, 1061
ApoI RAATTY 3 cut(s) 275, 399, 908
Asp700I GAANNNNTTC 1 cut(s) 215
AspLEI GCGC 2 cut(s) 880, 950
AspS9I GGNCC 2 cut(s) 956, 1097
AsuC2I CCSGG 3 cut(s) 12, 98, 675
AsuHPI GGTGA 4 cut(s) 619, 797, 809, 878
AvaII GGWCC 2 cut(s) 956, 1097
BaeGI GKGCMC 1 cut(s) 675
BanI GGYRCC 1 cut(s) 670
BanII GRGCYC 1 cut(s) 83
BbsI GAAGAC 2 cut(s) 34, 396
Bbv12I GWGCWC 1 cut(s) 83
BbvCI CCTCAGC 1 cut(s) 1080
BbvI GCAGC 2 cut(s) 814, 1048
BccI CCATC 1 cut(s) 1069
BceAI ACGGC 2 cut(s) 262, 1116
BciT130I CCWGG 1 cut(s) 605
BciVI GTATCC 1 cut(s) 18
BclI TGATCA 1 cut(s) 609
BcnI CCSGG 3 cut(s) 12, 98, 675
BfaI CTAG 1 cut(s) 465
BfmI CTRYAG 1 cut(s) 490
BfuI GTATCC 1 cut(s) 18
BglII AGATCT 1 cut(s) 175
BisI GCNGC 4 cut(s) 250, 803, 946, 1062
BlsI GCNGC 4 cut(s) 251, 804, 947, 1063
Bme1390I CCNGG 4 cut(s) 12, 98, 605, 675
Bme18I GGWCC 2 cut(s) 956, 1097
BmgT120I GGNCC 2 cut(s) 956, 1097
BmiI GGNNCC 1 cut(s) 672
BmrFI CCNGG 4 cut(s) 12, 98, 605, 675
BmrI ACTGGG 1 cut(s) 680
BmsI GCATC 2 cut(s) 588, 683
BmuI ACTGGG 1 cut(s) 680
BoxI GACNNNNGTC 1 cut(s) 931
BpiI GAAGAC 2 cut(s) 34, 396
BplI GAGNNNNNCTC 2 cut(s) 801, 833
BpmI CTGGAG 2 cut(s) 812, 1161
Bpu10I CCTNAGC 1 cut(s) 1080
BpuMI CCSGG 3 cut(s) 12, 98, 675
Bsa29I ATCGAT 1 cut(s) 110
BsaJI CCNNGG 2 cut(s) 603, 793
Bsc4I CCNNNNNNNGG 4 cut(s) 203, 308, 755, 978
Bse1I ACTGG 3 cut(s) 148, 235, 686
Bse3DI GCAATG 1 cut(s) 132
BseBI CCWGG 1 cut(s) 605
BseCI ATCGAT 1 cut(s) 110
BseDI CCNNGG 2 cut(s) 603, 793
BseGI GGATG 2 cut(s) 698, 764
BseLI CCNNNNNNNGG 4 cut(s) 203, 308, 755, 978
BseMI GCAATG 1 cut(s) 132
BseMII CTCAG 4 cut(s) 177, 711, 1047, 1071
BseNI ACTGG 3 cut(s) 148, 235, 686
BseRI GAGGAG 1 cut(s) 26
BseSI GKGCMC 1 cut(s) 675
BseX3I CGGCCG 1 cut(s) 247
BseXI GCAGC 2 cut(s) 814, 1048
Bsh1236I CGCG 3 cut(s) 350, 557, 878
Bsh1285I CGRYCG 1 cut(s) 250
BshFI GGCC 2 cut(s) 159, 249
BshNI GGYRCC 1 cut(s) 670
BshVI ATCGAT 1 cut(s) 110
BsiEI CGRYCG 1 cut(s) 250
BsiHKAI GWGCWC 1 cut(s) 83
BsiSI CCGG 3 cut(s) 12, 97, 675
BslFI GGGAC 2 cut(s) 1003, 1052
BslI CCNNNNNNNGG 4 cut(s) 203, 308, 755, 978
BsmFI GGGAC 2 cut(s) 1003, 1052
BsnI GGCC 2 cut(s) 159, 249
Bsp1286I GDGCHC 2 cut(s) 83, 675
Bsp143I GATC 9 cut(s) 111, 166, 175, 311, 585, 609, 642, 745, 1015
Bsp19I CCATGG 1 cut(s) 793
BspACI CCGC 6 cut(s) 250, 290, 350, 366, 936, 945
BspANI GGCC 2 cut(s) 159, 249
BspCNI CTCAG 4 cut(s) 176, 710, 1048, 1072
BspDI ATCGAT 1 cut(s) 110
BspFNI CGCG 3 cut(s) 350, 557, 878
BspHI TCATGA 1 cut(s) 918
BspLI GGNNCC 1 cut(s) 672
BspPI GGATC 4 cut(s) 161, 319, 740, 1010
BspT107I GGYRCC 1 cut(s) 670
BsrDI GCAATG 1 cut(s) 132
BsrI ACTGG 3 cut(s) 148, 235, 686
BssECI CCNNGG 2 cut(s) 603, 793
BssMI GATC 9 cut(s) 111, 166, 175, 311, 585, 609, 642, 745, 1015
BssNAI GTATAC 1 cut(s) 1088
BssT1I CCWWGG 1 cut(s) 793
Bst1107I GTATAC 1 cut(s) 1088
Bst2UI CCWGG 1 cut(s) 605
Bst4CI ACNGT 7 cut(s) 201, 491, 960, 973, 1043, 1101, 1154
Bst6I CTCTTC 1 cut(s) 576
BstDEI CTNAG 6 cut(s) 90, 163, 697, 806, 1056, 1080
BstDSI CCRYGG 1 cut(s) 793
BstENI CCTNNNNNAGG 1 cut(s) 753
BstF5I GGATG 2 cut(s) 698, 764
BstFNI CGCG 3 cut(s) 350, 557, 878
BstHHI GCGC 2 cut(s) 880, 950
BstKTI GATC 9 cut(s) 114, 169, 178, 314, 588, 612, 645, 748, 1018
BstMBI GATC 9 cut(s) 111, 166, 175, 311, 585, 609, 642, 745, 1015
BstMCI CGRYCG 1 cut(s) 250
BstMWI GCNNNNNNNGC 1 cut(s) 474
BstNI CCWGG 1 cut(s) 605
BstPAI GACNNNNGTC 1 cut(s) 931
BstSCI CCNGG 4 cut(s) 10, 96, 603, 673
BstSFI CTRYAG 1 cut(s) 490
BstSLI GKGCMC 1 cut(s) 675
BstUI CGCG 3 cut(s) 350, 557, 878
BstV1I GCAGC 2 cut(s) 814, 1048
BstV2I GAAGAC 2 cut(s) 34, 396
BstX2I RGATCY 3 cut(s) 166, 175, 311
BstXI CCANNNNNNTGG 1 cut(s) 230
BstYI RGATCY 3 cut(s) 166, 175, 311
BstZ17I GTATAC 1 cut(s) 1088
BstZI CGGCCG 1 cut(s) 247
Bsu15I ATCGAT 1 cut(s) 110
BsuI GTATCC 1 cut(s) 18
BsuRI GGCC 2 cut(s) 159, 249
BsuTUI ATCGAT 1 cut(s) 110
BtgI CCRYGG 1 cut(s) 793
BtgZI GCGATG 1 cut(s) 253
BtsCI GGATG 2 cut(s) 698, 764
BtsIMutI CAGTG 2 cut(s) 141, 1159
CciI TCATGA 1 cut(s) 918
CfoI GCGC 2 cut(s) 880, 950
Cfr13I GGNCC 2 cut(s) 956, 1097
ClaI ATCGAT 1 cut(s) 110
Csp6I GTAC 3 cut(s) 838, 961, 1066
CspCI CAANNNNNGTGG 2 cut(s) 1011, 1046
CviAII CATG 6 cut(s) 187, 523, 733, 794, 845, 919
CviQI GTAC 3 cut(s) 838, 961, 1066
DdeI CTNAG 6 cut(s) 90, 163, 697, 806, 1056, 1080
DpnI GATC 9 cut(s) 113, 168, 177, 313, 587, 611, 644, 747, 1017
DpnII GATC 9 cut(s) 111, 166, 175, 311, 585, 609, 642, 745, 1015
EaeI YGGCCR 1 cut(s) 247
EagI CGGCCG 1 cut(s) 247
Eam1104I CTCTTC 1 cut(s) 576
EarI CTCTTC 1 cut(s) 576
Ecl136II GAGCTC 1 cut(s) 81
EclXI CGGCCG 1 cut(s) 247
Eco130I CCWWGG 1 cut(s) 793
Eco24I GRGCYC 1 cut(s) 83
Eco47I GGWCC 2 cut(s) 956, 1097
Eco52I CGGCCG 1 cut(s) 247
Eco53kI GAGCTC 1 cut(s) 81
Eco57I CTGAAG 1 cut(s) 468
EcoICRI GAGCTC 1 cut(s) 81
EcoNI CCTNNNNNAGG 1 cut(s) 753
EcoRI GAATTC 2 cut(s) 275, 399
EcoRII CCWGG 1 cut(s) 603
EcoT14I CCWWGG 1 cut(s) 793
EcoT38I GRGCYC 1 cut(s) 83
ErhI CCWWGG 1 cut(s) 793
FaeI CATG 6 cut(s) 190, 526, 736, 797, 848, 922
FaqI GGGAC 2 cut(s) 1003, 1052
FatI CATG 6 cut(s) 186, 522, 732, 793, 844, 918
FauNDI CATATG 2 cut(s) 470, 1050
FbaI TGATCA 1 cut(s) 609
FblI GTMKAC 1 cut(s) 1087
Fnu4HI GCNGC 4 cut(s) 250, 803, 946, 1062
FokI GGATG 2 cut(s) 705, 771
FriOI GRGCYC 1 cut(s) 83
Fsp4HI GCNGC 4 cut(s) 250, 803, 946, 1062
FspBI CTAG 1 cut(s) 465
GlaI GCGC 2 cut(s) 879, 949
GluI GCNGC 4 cut(s) 250, 803, 946, 1062
GsuI CTGGAG 2 cut(s) 812, 1161
HaeIII GGCC 2 cut(s) 159, 249
HapII CCGG 3 cut(s) 12, 97, 675
HhaI GCGC 2 cut(s) 880, 950
Hin1II CATG 6 cut(s) 190, 526, 736, 797, 848, 922
Hin6I GCGC 2 cut(s) 878, 948
HinP1I GCGC 2 cut(s) 878, 948
HincII GTYRAC 1 cut(s) 850
HindII GTYRAC 1 cut(s) 850
HinfI GANTC 5 cut(s) 128, 517, 825, 915, 1108
HpaI GTTAAC 1 cut(s) 850
HpaII CCGG 3 cut(s) 12, 97, 675
HphI GGTGA 4 cut(s) 619, 797, 809, 878
Hpy166II GTNNAC 5 cut(s) 287, 461, 568, 850, 1088
Hpy188I TCNGA 7 cut(s) 85, 125, 166, 487, 692, 914, 1165
Hpy188III TCNNGA 6 cut(s) 25, 386, 829, 896, 919, 1140
Hpy8I GTNNAC 5 cut(s) 287, 461, 568, 850, 1088
HpyAV CCTTC 2 cut(s) 492, 1029
HpyCH4III ACNGT 7 cut(s) 201, 491, 960, 973, 1043, 1101, 1154
HpyCH4IV ACGT 2 cut(s) 75, 376
HpyCH4V TGCA 2 cut(s) 323, 629
HpyF10VI GCNNNNNNNGC 1 cut(s) 474
HpyF3I CTNAG 6 cut(s) 90, 163, 697, 806, 1056, 1080
HpySE526I ACGT 2 cut(s) 75, 376
Hsp92II CATG 6 cut(s) 190, 526, 736, 797, 848, 922
HspAI GCGC 2 cut(s) 878, 948
Ksp22I TGATCA 1 cut(s) 609
KspAI GTTAAC 1 cut(s) 850
Kzo9I GATC 9 cut(s) 111, 166, 175, 311, 585, 609, 642, 745, 1015
LmnI GCTCC 3 cut(s) 39, 359, 592
Lsp1109I GCAGC 2 cut(s) 814, 1048
LweI GCATC 2 cut(s) 588, 683
MaeI CTAG 1 cut(s) 465
MaeII ACGT 2 cut(s) 75, 376
MaeIII GTNAC 1 cut(s) 67
MalI GATC 9 cut(s) 113, 168, 177, 313, 587, 611, 644, 747, 1017
MboI GATC 9 cut(s) 111, 166, 175, 311, 585, 609, 642, 745, 1015
MboII GAAGA 9 cut(s) 34, 170, 208, 401, 553, 593, 615, 669, 749
MflI RGATCY 3 cut(s) 166, 175, 311
MhlI GDGCHC 2 cut(s) 83, 675
MluCI AATT 5 cut(s) 275, 343, 399, 531, 908
MluI ACGCGT 1 cut(s) 555
MlyI GAGTC 1 cut(s) 819
MmeI TCCRAC 2 cut(s) 267, 670
MroXI GAANNNNTTC 1 cut(s) 215
MseI TTAA 5 cut(s) 342, 441, 752, 849, 1146
MslI CAYNNNNRTG 2 cut(s) 74, 419
MspI CCGG 3 cut(s) 12, 97, 675
MspR9I CCNGG 4 cut(s) 12, 98, 605, 675
MvaI CCWGG 1 cut(s) 605
MvnI CGCG 3 cut(s) 350, 557, 878
MwoI GCNNNNNNNGC 1 cut(s) 474
NciI CCSGG 3 cut(s) 12, 98, 675
NcoI CCATGG 1 cut(s) 793
NdeI CATATG 2 cut(s) 470, 1050
NdeII GATC 9 cut(s) 111, 166, 175, 311, 585, 609, 642, 745, 1015
NlaIII CATG 6 cut(s) 190, 526, 736, 797, 848, 922
NlaIV GGNNCC 1 cut(s) 672
NmuCI GTSAC 1 cut(s) 67
OliI CACNNNNGTG 2 cut(s) 74, 419
PagI TCATGA 1 cut(s) 918
PcsI WCGNNNNNNNCGW 1 cut(s) 561
PdmI GAANNNNTTC 1 cut(s) 215
PfeI GAWTC 4 cut(s) 128, 517, 915, 1108
PflMI CCANNNNNTGG 2 cut(s) 203, 308
PkrI GCNGC 4 cut(s) 251, 804, 947, 1063
PleI GAGTC 1 cut(s) 819
PpsI GAGTC 1 cut(s) 819
PshAI GACNNNNGTC 1 cut(s) 931
Psp124BI GAGCTC 1 cut(s) 83
Psp1406I AACGTT 1 cut(s) 376
Psp6I CCWGG 1 cut(s) 603
PspGI CCWGG 1 cut(s) 603
PspN4I GGNNCC 1 cut(s) 672
PspPI GGNCC 2 cut(s) 956, 1097
PsuI RGATCY 3 cut(s) 166, 175, 311
RsaI GTAC 3 cut(s) 839, 962, 1067
RsaNI GTAC 3 cut(s) 838, 961, 1066
RseI CAYNNNNRTG 2 cut(s) 74, 419
SacI GAGCTC 1 cut(s) 83
SaqAI TTAA 5 cut(s) 342, 441, 752, 849, 1146
SatI GCNGC 4 cut(s) 250, 803, 946, 1062
Sau3AI GATC 9 cut(s) 111, 166, 175, 311, 585, 609, 642, 745, 1015
Sau96I GGNCC 2 cut(s) 956, 1097
SchI GAGTC 1 cut(s) 819
ScrFI CCNGG 4 cut(s) 12, 98, 605, 675
SduI GDGCHC 2 cut(s) 83, 675
SfaNI GCATC 2 cut(s) 588, 683
SfcI CTRYAG 1 cut(s) 490
SinI GGWCC 2 cut(s) 956, 1097
SmiMI CAYNNNNRTG 2 cut(s) 74, 419
Sse9I AATT 5 cut(s) 275, 343, 399, 531, 908
SsiI CCGC 6 cut(s) 250, 290, 350, 366, 936, 945
SspMI CTAG 1 cut(s) 465
SstI GAGCTC 1 cut(s) 83
StyD4I CCNGG 4 cut(s) 10, 96, 603, 673
StyI CCWWGG 1 cut(s) 793
TaaI ACNGT 7 cut(s) 201, 491, 960, 973, 1043, 1101, 1154
TaiI ACGT 2 cut(s) 78, 379
TaqI TCGA 1 cut(s) 110
TasI AATT 5 cut(s) 275, 343, 399, 531, 908
TauI GCSGC 2 cut(s) 252, 948
TfiI GAWTC 4 cut(s) 128, 517, 915, 1108
Tru1I TTAA 5 cut(s) 342, 441, 752, 849, 1146
Tru9I TTAA 5 cut(s) 342, 441, 752, 849, 1146
TscAI CASTG 2 cut(s) 148, 1159
TseFI GTSAC 1 cut(s) 67
TseI GCWGC 2 cut(s) 802, 1061
Tsp45I GTSAC 1 cut(s) 67
TspDTI ATGAA 7 cut(s) 34, 44, 256, 392, 907, 935, 1037
TspGWI ACGGA 1 cut(s) 650
TspRI CASTG 2 cut(s) 148, 1159
Van91I CCANNNNNTGG 2 cut(s) 203, 308
VpaK11BI GGWCC 2 cut(s) 956, 1097
XagI CCTNNNNNAGG 1 cut(s) 753
XapI RAATTY 3 cut(s) 275, 399, 908
XcmI CCANNNNNNNNNTGG 1 cut(s) 227
XmiI GTMKAC 1 cut(s) 1087
XmnI GAANNNNTTC 1 cut(s) 215
XspI CTAG 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.