AT1G28590

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001486
Physical Location & Seq
Reverse (-)
10047276 .. 10049343
2068 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G28590.1

Sequence Viewer

Length: 1212 bp
ATGGCATCTTTAGATTCTCTTCCGGCGATGAAGCTCGTGAGATTCATATTATCTACCCTTTTGGTCACTTCTGTGAACTCGCAAACGCAATGCCGGAATTTCAAATCAATCATTAGTTTCGGAGATTCAATTGCTGACACTGGAAACTTGCTTGGTCTCTCGGATCCTAACGATCTTCCTGCTTCCGCGTTTCCGCCATACGGAGAAACCTTCTTCCACCACCCAACCGGTCGTTACTCCGACGGCCGCCTCATCATTGATTTCATTGCTGAATTCTTGGGGTTTCCGCTTGTGCCTCCTTTTTATGGATGTCAAAATGCAAACTTTAAAAAGGGAGTTAATTTCGCGGTCGCGGGAGCAACTGCATTAGAACCTTCCTTTCTAGAGGAGAGAGGAATTCATTCTACTATCACCAACGTCAGTCTAAGCGTTCAGCTTAGGAGCTTCACGGAAAGTTTACCTAACTTATGTGGCTCACCTTCAGATTGTAGAGATATGATTGAAAATGCTTTGATACTCATGGGAGAGATCGGAGGAAATGATTATAATTTTGCACTCTTCCAACGCAAACCCGTTAAGGAAGTTGAAGAGCTGGTACCGTTTGTGATCGCTACTATTTCTTCTGCAATCACGGAATTGGTTTGCATGGGAGGAAGAACATTCCTAGTTCCTGGAAATTTCCCGATCGGATACTCAGCATCCTATTTGACATTATACAAAACATCAAACAAGGAAGAGTATGATCCTCTAACAGGATGTTTGAAATGGCTTAACGATTTCTCAGAATACTACAATAAGCAGCTTCAGGAAGAACTCAACGGACTCAGGAAGTTGTACCCTCATGTCAACATCATATATGCTGATTACTACAACGCTTTGTTGCGCCTTTTCCAAGAACCAGCCAAATTCGGGTTTATGAACAGACCCTTGCCCGCTTGTTGCGGTGTAGGAGGATCTTACAACTTCAACTTTAGTAGAAGATGTGGGAGTGTAGGAGTTGAATACTGCGATGATCCTTCACAGTATGTGAATTATGATGGCATTCATATGACGGAGGCGGCATACAGATTGATATCTGAGGGCTTACTCAAGGGACCGTATGCTATTCCTCCTTTCAAGTGGTCTTGCCTCAGCTCCGAGATTATGAATAAGATGTCATTAGATACTCAGATTCTTGATGAACAGCTACTACAAGGTTGTCTGAAAGTTTGA

Protein Analysis

403

Amino Acids

44.81

Weight (kDa)

5.11

Isoelectric Point (pI)

48.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 37 - 360 6.4e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 546
Acc65I GGTACC 1 cut(s) 595
AccB1I GGYRCC 1 cut(s) 595
AccII CGCG 3 cut(s) 188, 347, 353
AciI CCGC 9 cut(s) 186, 194, 247, 287, 347, 353, 933, 942, 1058
AclWI GGATC 5 cut(s) 158, 171, 737, 961, 1007
AcoI YGGCCR 1 cut(s) 244
AcsI RAATTY 5 cut(s) 97, 272, 396, 676, 905
AcuI CTGAAG 2 cut(s) 465, 788
AfaI GTAC 2 cut(s) 597, 836
AfiI CCNNNNNNNGG 4 cut(s) 200, 305, 752, 909
AgeI ACCGGT 1 cut(s) 227
AgsI TTSAA 8 cut(s) 103, 129, 503, 587, 763, 967, 1001, 1117
AjnI CCWGG 1 cut(s) 670
AleI CACNNNNGTG 1 cut(s) 71
AluBI AGCT 7 cut(s) 34, 436, 444, 592, 802, 1134, 1186
AluI AGCT 7 cut(s) 34, 436, 444, 592, 802, 1134, 1186
Alw26I GTCTC 1 cut(s) 161
AlwI GGATC 5 cut(s) 158, 171, 737, 961, 1007
AoxI GGCC 1 cut(s) 244
ApeKI GCWGC 1 cut(s) 799
ApoI RAATTY 5 cut(s) 97, 272, 396, 676, 905
AsiGI ACCGGT 1 cut(s) 227
Asp700I GAANNNNTTC 1 cut(s) 400
Asp718I GGTACC 1 cut(s) 595
AspLEI GCGC 1 cut(s) 885
AspS9I GGNCC 1 cut(s) 1094
AsuHPI GGTGA 2 cut(s) 403, 468
AvaII GGWCC 1 cut(s) 1094
BamHI GGATCC 1 cut(s) 163
BanI GGYRCC 1 cut(s) 595
BarI GAAGNNNNNNTAC 2 cut(s) 579, 611
BauI CACGAG 1 cut(s) 35
BbvCI CCTCAGC 1 cut(s) 1130
BbvI GCAGC 1 cut(s) 811
BccI CCATC 1 cut(s) 1031
BceAI ACGGC 1 cut(s) 259
BcgI CGANNNNNNTGC 2 cut(s) 161, 195
BciT130I CCWGG 1 cut(s) 672
BciVI GTATCC 1 cut(s) 683
BcoDI GTCTC 1 cut(s) 161
BfaI CTAG 2 cut(s) 383, 665
BfuI GTATCC 1 cut(s) 683
BisI GCNGC 3 cut(s) 247, 800, 1059
BlsI GCNGC 3 cut(s) 248, 801, 1060
Bme1390I CCNGG 1 cut(s) 672
Bme18I GGWCC 1 cut(s) 1094
BmgT120I GGNCC 1 cut(s) 1094
BmiI GGNNCC 3 cut(s) 165, 597, 1095
BmrFI CCNGG 1 cut(s) 672
BmsI GCATC 2 cut(s) 14, 707
Bpu10I CCTNAGC 2 cut(s) 437, 1130
BpuEI CTTGAG 1 cut(s) 1073
BsaBI GATNNNNATC 1 cut(s) 1072
BsaI GGTCTC 1 cut(s) 161
BsaWI WCCGGW 1 cut(s) 227
Bsc4I CCNNNNNNNGG 4 cut(s) 200, 305, 752, 909
Bse118I RCCGGY 1 cut(s) 227
Bse1I ACTGG 1 cut(s) 145
Bse3DI GCAATG 2 cut(s) 95, 264
Bse8I GATNNNNATC 1 cut(s) 1072
BseBI CCWGG 1 cut(s) 672
BseGI GGATG 3 cut(s) 314, 698, 761
BseJI GATNNNNATC 1 cut(s) 1072
BseLI CCNNNNNNNGG 4 cut(s) 200, 305, 752, 909
BseMI GCAATG 2 cut(s) 95, 264
BseMII CTCAG 6 cut(s) 708, 795, 838, 1068, 1144, 1181
BseNI ACTGG 1 cut(s) 145
BseRI GAGGAG 1 cut(s) 401
BseX3I CGGCCG 1 cut(s) 244
BseXI GCAGC 1 cut(s) 811
Bsh1236I CGCG 3 cut(s) 188, 347, 353
Bsh1285I CGRYCG 4 cut(s) 232, 247, 351, 687
BshFI GGCC 1 cut(s) 246
BshNI GGYRCC 1 cut(s) 595
BshTI ACCGGT 1 cut(s) 227
BsiEI CGRYCG 4 cut(s) 232, 247, 351, 687
BsiSI CCGG 3 cut(s) 23, 94, 228
BslFI GGGAC 1 cut(s) 1107
BslI CCNNNNNNNGG 4 cut(s) 200, 305, 752, 909
BsmAI GTCTC 1 cut(s) 161
BsmFI GGGAC 1 cut(s) 1107
BsmI GAATGC 1 cut(s) 1041
BsnI GGCC 1 cut(s) 246
Bso31I GGTCTC 1 cut(s) 161
Bsp143I GATC 8 cut(s) 163, 172, 528, 606, 684, 742, 953, 1012
BspACI CCGC 9 cut(s) 186, 194, 247, 287, 347, 353, 933, 942, 1058
BspANI GGCC 1 cut(s) 246
BspCNI CTCAG 6 cut(s) 707, 794, 837, 1069, 1143, 1180
BspFNI CGCG 3 cut(s) 188, 347, 353
BspLI GGNNCC 3 cut(s) 165, 597, 1095
BspPI GGATC 5 cut(s) 158, 171, 737, 961, 1007
BspQI GCTCTTC 1 cut(s) 582
BspT107I GGYRCC 1 cut(s) 595
BspTNI GGTCTC 1 cut(s) 161
BsrDI GCAATG 2 cut(s) 95, 264
BsrFI RCCGGY 1 cut(s) 227
BsrI ACTGG 1 cut(s) 145
BssAI RCCGGY 1 cut(s) 227
BssMI GATC 8 cut(s) 163, 172, 528, 606, 684, 742, 953, 1012
BssSI CACGAG 1 cut(s) 35
Bst2BI CACGAG 1 cut(s) 35
Bst2UI CCWGG 1 cut(s) 672
Bst4CI ACNGT 3 cut(s) 600, 1023, 1098
Bst6I CTCTTC 4 cut(s) 24, 563, 582, 729
BstC8I GCNNGC 1 cut(s) 933
BstDEI CTNAG 8 cut(s) 425, 437, 694, 781, 824, 1077, 1130, 1167
BstENI CCTNNNNNAGG 1 cut(s) 750
BstF5I GGATG 3 cut(s) 314, 698, 761
BstFNI CGCG 3 cut(s) 188, 347, 353
BstHHI GCGC 1 cut(s) 885
BstKTI GATC 8 cut(s) 166, 175, 531, 609, 687, 745, 956, 1015
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 8 cut(s) 163, 172, 528, 606, 684, 742, 953, 1012
BstMCI CGRYCG 4 cut(s) 232, 247, 351, 687
BstNI CCWGG 1 cut(s) 672
BstSCI CCNGG 1 cut(s) 670
BstUI CGCG 3 cut(s) 188, 347, 353
BstV1I GCAGC 1 cut(s) 811
BstX2I RGATCY 2 cut(s) 163, 953
BstYI RGATCY 2 cut(s) 163, 953
BstZI CGGCCG 1 cut(s) 244
BsuI GTATCC 1 cut(s) 683
BsuRI GGCC 1 cut(s) 246
BtgZI GCGATG 2 cut(s) 41, 1023
BtsCI GGATG 3 cut(s) 314, 698, 761
BtsIMutI CAGTG 1 cut(s) 138
Cac8I GCNNGC 1 cut(s) 933
CfoI GCGC 1 cut(s) 885
Cfr10I RCCGGY 1 cut(s) 227
Cfr13I GGNCC 1 cut(s) 1094
Csp6I GTAC 2 cut(s) 596, 835
CspAI ACCGGT 1 cut(s) 227
CviAII CATG 3 cut(s) 520, 646, 842
CviQI GTAC 2 cut(s) 596, 835
DdeI CTNAG 8 cut(s) 425, 437, 694, 781, 824, 1077, 1130, 1167
DpnI GATC 8 cut(s) 165, 174, 530, 608, 686, 744, 955, 1014
DpnII GATC 8 cut(s) 163, 172, 528, 606, 684, 742, 953, 1012
DraI TTTAAA 1 cut(s) 328
EaeI YGGCCR 1 cut(s) 244
EagI CGGCCG 1 cut(s) 244
Eam1104I CTCTTC 4 cut(s) 24, 563, 582, 729
EarI CTCTTC 4 cut(s) 24, 563, 582, 729
EciI GGCGGA 1 cut(s) 183
EclXI CGGCCG 1 cut(s) 244
Eco31I GGTCTC 1 cut(s) 161
Eco32I GATATC 1 cut(s) 1074
Eco47I GGWCC 1 cut(s) 1094
Eco52I CGGCCG 1 cut(s) 244
Eco57I CTGAAG 2 cut(s) 465, 788
EcoNI CCTNNNNNAGG 1 cut(s) 750
EcoRI GAATTC 2 cut(s) 272, 396
EcoRII CCWGG 1 cut(s) 670
EcoRV GATATC 1 cut(s) 1074
FaeI CATG 3 cut(s) 523, 649, 845
FaqI GGGAC 1 cut(s) 1107
FatI CATG 3 cut(s) 519, 645, 841
FauI CCCGC 2 cut(s) 346, 940
FauNDI CATATG 1 cut(s) 1047
Fnu4HI GCNGC 3 cut(s) 247, 800, 1059
FokI GGATG 3 cut(s) 321, 685, 768
Fsp4HI GCNGC 3 cut(s) 247, 800, 1059
FspBI CTAG 2 cut(s) 383, 665
GlaI GCGC 1 cut(s) 884
GluI GCNGC 3 cut(s) 247, 800, 1059
HaeIII GGCC 1 cut(s) 246
HapII CCGG 3 cut(s) 23, 94, 228
HhaI GCGC 1 cut(s) 885
Hin1II CATG 3 cut(s) 523, 649, 845
Hin6I GCGC 1 cut(s) 883
HinP1I GCGC 1 cut(s) 883
HincII GTYRAC 1 cut(s) 847
HindII GTYRAC 1 cut(s) 847
HinfI GANTC 5 cut(s) 14, 42, 125, 822, 1171
HpaII CCGG 3 cut(s) 23, 94, 228
HphI GGTGA 2 cut(s) 403, 468
Hpy166II GTNNAC 3 cut(s) 76, 458, 847
Hpy188III TCNNGA 6 cut(s) 37, 383, 682, 806, 826, 1175
Hpy8I GTNNAC 3 cut(s) 76, 458, 847
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 4 cut(s) 220, 384, 489, 1026
HpyCH4III ACNGT 3 cut(s) 600, 1023, 1098
HpyCH4IV ACGT 1 cut(s) 417
HpyCH4V TGCA 5 cut(s) 320, 365, 554, 626, 645
HpyF3I CTNAG 8 cut(s) 425, 437, 694, 781, 824, 1077, 1130, 1167
HpySE526I ACGT 1 cut(s) 417
Hsp92II CATG 3 cut(s) 523, 649, 845
HspAI GCGC 1 cut(s) 883
KpnI GGTACC 1 cut(s) 599
Kzo9I GATC 8 cut(s) 163, 172, 528, 606, 684, 742, 953, 1012
LguI GCTCTTC 1 cut(s) 582
LmnI GCTCC 3 cut(s) 356, 441, 1139
Lsp1109I GCAGC 1 cut(s) 811
LweI GCATC 2 cut(s) 14, 707
MaeI CTAG 2 cut(s) 383, 665
MaeII ACGT 1 cut(s) 417
MaeIII GTNAC 2 cut(s) 64, 233
MalI GATC 8 cut(s) 165, 174, 530, 608, 686, 744, 955, 1014
MboI GATC 8 cut(s) 163, 172, 528, 606, 684, 742, 953, 1012
MfeI CAATTG 1 cut(s) 129
MflI RGATCY 2 cut(s) 163, 953
MlyI GAGTC 1 cut(s) 816
MmeI TCCRAC 2 cut(s) 264, 586
MroXI GAANNNNTTC 1 cut(s) 400
MseI TTAA 4 cut(s) 327, 339, 576, 771
MslI CAYNNNNRTG 2 cut(s) 71, 1046
MspI CCGG 3 cut(s) 23, 94, 228
MspR9I CCNGG 1 cut(s) 672
MunI CAATTG 1 cut(s) 129
Mva1269I GAATGC 1 cut(s) 1041
MvaI CCWGG 1 cut(s) 672
MvnI CGCG 3 cut(s) 188, 347, 353
NdeI CATATG 1 cut(s) 1047
NdeII GATC 8 cut(s) 163, 172, 528, 606, 684, 742, 953, 1012
NlaIII CATG 3 cut(s) 523, 649, 845
NlaIV GGNNCC 3 cut(s) 165, 597, 1095
NmuCI GTSAC 1 cut(s) 64
OliI CACNNNNGTG 1 cut(s) 71
PciSI GCTCTTC 1 cut(s) 582
PctI GAATGC 1 cut(s) 1041
PdmI GAANNNNTTC 1 cut(s) 400
PfeI GAWTC 4 cut(s) 14, 42, 125, 1171
PfoI TCCNGGA 1 cut(s) 670
PinAI ACCGGT 1 cut(s) 227
PkrI GCNGC 3 cut(s) 248, 801, 1060
Ple19I CGATCG 1 cut(s) 687
PleI GAGTC 1 cut(s) 816
PpsI GAGTC 1 cut(s) 816
PsiI TTATAA 1 cut(s) 546
Psp6I CCWGG 1 cut(s) 670
PspGI CCWGG 1 cut(s) 670
PspN4I GGNNCC 3 cut(s) 165, 597, 1095
PspPI GGNCC 1 cut(s) 1094
PsrI GAACNNNNNNTAC 2 cut(s) 1173, 1205
PsuI RGATCY 2 cut(s) 163, 953
PvuI CGATCG 1 cut(s) 687
RsaI GTAC 2 cut(s) 597, 836
RsaNI GTAC 2 cut(s) 596, 835
RseI CAYNNNNRTG 2 cut(s) 71, 1046
SapI GCTCTTC 1 cut(s) 582
SaqAI TTAA 4 cut(s) 327, 339, 576, 771
SatI GCNGC 3 cut(s) 247, 800, 1059
Sau3AI GATC 8 cut(s) 163, 172, 528, 606, 684, 742, 953, 1012
Sau96I GGNCC 1 cut(s) 1094
SchI GAGTC 1 cut(s) 816
ScrFI CCNGG 1 cut(s) 672
SfaNI GCATC 2 cut(s) 14, 707
SinI GGWCC 1 cut(s) 1094
SmiMI CAYNNNNRTG 2 cut(s) 71, 1046
SmlI CTYRAG 1 cut(s) 1088
SmoI CTYRAG 1 cut(s) 1088
SsiI CCGC 9 cut(s) 186, 194, 247, 287, 347, 353, 933, 942, 1058
SspMI CTAG 2 cut(s) 383, 665
StyD4I CCNGG 1 cut(s) 670
TaaI ACNGT 3 cut(s) 600, 1023, 1098
TaiI ACGT 1 cut(s) 420
TauI GCSGC 2 cut(s) 249, 1061
TfiI GAWTC 4 cut(s) 14, 42, 125, 1171
Tru1I TTAA 4 cut(s) 327, 339, 576, 771
Tru9I TTAA 4 cut(s) 327, 339, 576, 771
TscAI CASTG 1 cut(s) 145
TseFI GTSAC 1 cut(s) 64
TseI GCWGC 1 cut(s) 799
Tsp45I GTSAC 1 cut(s) 64
TspDTI ATGAA 8 cut(s) 34, 44, 253, 389, 932, 1034, 1160, 1194
TspGWI ACGGA 5 cut(s) 216, 464, 647, 834, 1067
TspRI CASTG 1 cut(s) 145
VpaK11BI GGWCC 1 cut(s) 1094
XagI CCTNNNNNAGG 1 cut(s) 750
XapI RAATTY 5 cut(s) 97, 272, 396, 676, 905
XbaI TCTAGA 1 cut(s) 382
XmnI GAANNNNTTC 1 cut(s) 400
XspI CTAG 2 cut(s) 383, 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.