AT1G28640

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001486
Physical Location & Seq
Reverse (-)
10067563 .. 10069177
1615 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G28640.1

Sequence Viewer

Length: 1170 bp
ATGGCTTCTTCACTGGAGAAGCTTATTTCAAGCTTTCTACTTGTCTTGTACTCCACCACCATCATTGTTGCTTCATCAGAATCTCGATGTAGGCGTTTTAAATCGATCATCAGCTTTGGTGATTCCATCGCCGACACCGGAAATTATCTCCATCTCTCTGATGTTAACCACCTTCCTCAATCCGCCTTTCTTCCTTATGGCGAAAGCTTCTTCCATCCTCCCTCCGGCCGTTACTCGGACGGCCGTCTCATCATCGACTTTATAGCTGAATTCTTGGGACTACCGTACGTACCATCTTATTTTGGATCCCAAAACGTGAGCTTTGATCAAGGTATCAATTTCGCGGTGTATGGTGCAACTGCATTGGATCGTGTGTTTCTTGTGGGAAAAGGAATTGAATCTGATTTCACCAATGTTAGTTTAAGTGTTCAGCTTAACATCTTCAAACAGATTTTACCTAATCTTTGCACCTCGTCTTCTCATTGCAGAGAGATGCTTGGAGACTCGCTGATACTTATGGGTGAGATTGGAGTAAATGACTATAATTACCCTTTCTTTGAAGGCAAAAGTATCAATGAAATCAAACAGCTTGTTCCTCTAGTCATCAAAGCTATTTCTTCTGCTATTGTGGATTTGATTGATTTAGGGGGCAAAACGTTTCTGGTCCCTGGAAACTTCCCATTAGGATGCTATCCCGCGTATCTCACTCTATTTCAGACCGCGGCAGAAGAAGACCACGACCCTTTCACAGGTTGTATCCCACGGCTCAATGAATTCGGAGAGTACCACAACGAACAGCTCAAGACAGAACTCAAAAGACTCCAAGAACTATACGATCATGTCAATATCATTTACGCCGACTACTACAACTCTTTGTTCCGGTTATATCAAGAACCAGTCAAATATGGGTTTAAGAACAGACCTTTGGCTGCTTGTTGCGGAGTCGGAGGTCAATACAACTTCACTATTGGTAAAGAGTGTGGACACAGAGGAGTTAGCTGTTGTCAAAATCCTTCTGAATATGTGAATTGGGACGGTTATCATTTAACCGAGGCCACACACCAGAAGATGGCTCAAGTTATACTCAACGGTACCTATGCATCACCTGCTTTCGACTGGTCCTGCTCTGGCTCTGAATCAGTGGATAAAGAGTATTCTTTCAGCAGTTAA

Protein Analysis

389

Amino Acids

43.37

Weight (kDa)

5.13

Isoelectric Point (pI)

43.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 36 - 360 6.9e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1114
Acc36I ACCTGC 1 cut(s) 1114
Acc65I GGTACC 1 cut(s) 1091
AccB1I GGYRCC 1 cut(s) 1091
AccB7I CCANNNNNTGG 1 cut(s) 1069
AccII CGCG 3 cut(s) 344, 698, 722
AciI CCGC 6 cut(s) 183, 344, 696, 720, 722, 939
AclI AACGTT 1 cut(s) 656
AclWI GGATC 3 cut(s) 300, 313, 375
AcoI YGGCCR 2 cut(s) 226, 241
AcsI RAATTY 2 cut(s) 269, 773
AfaI GTAC 5 cut(s) 50, 287, 291, 785, 1093
AfiI CCNNNNNNNGG 4 cut(s) 224, 235, 749, 1069
AgsI TTSAA 4 cut(s) 30, 398, 445, 560
AjnI CCWGG 1 cut(s) 667
AjuI GAANNNNNNNTTGG 2 cut(s) 908, 940
Alw26I GTCTC 2 cut(s) 251, 495
AlwI GGATC 3 cut(s) 300, 313, 375
AoxI GGCC 3 cut(s) 226, 241, 1053
ApeKI GCWGC 1 cut(s) 929
ApoI RAATTY 2 cut(s) 269, 773
Asp718I GGTACC 1 cut(s) 1091
AspS9I GGNCC 2 cut(s) 664, 1119
AsuHPI GGTGA 4 cut(s) 131, 400, 533, 1095
AvaII GGWCC 2 cut(s) 664, 1119
BamHI GGATCC 1 cut(s) 305
BanI GGYRCC 1 cut(s) 1091
BbsI GAAGAC 2 cut(s) 468, 738
BbvI GCAGC 1 cut(s) 916
BccI CCATC 6 cut(s) 68, 134, 159, 222, 301, 1063
BceAI ACGGC 4 cut(s) 213, 228, 256, 779
BciT130I CCWGG 1 cut(s) 669
BciVI GTATCC 1 cut(s) 767
BclI TGATCA 1 cut(s) 325
BcoDI GTCTC 2 cut(s) 251, 495
BfaI CTAG 1 cut(s) 599
BfuAI ACCTGC 1 cut(s) 1114
BfuI GTATCC 1 cut(s) 767
BisI GCNGC 2 cut(s) 723, 930
BlsI GCNGC 2 cut(s) 724, 931
Bme1390I CCNGG 1 cut(s) 669
Bme18I GGWCC 2 cut(s) 664, 1119
BmgT120I GGNCC 2 cut(s) 664, 1119
BmiI GGNNCC 3 cut(s) 307, 666, 1093
BmrFI CCNGG 1 cut(s) 669
BmsI GCATC 3 cut(s) 483, 677, 1109
BoxI GACNNNNGTC 1 cut(s) 243
BpiI GAAGAC 2 cut(s) 468, 738
BpmI CTGGAG 1 cut(s) 35
BpuEI CTTGAG 2 cut(s) 785, 1059
Bsa29I ATCGAT 1 cut(s) 104
BsaAI YACGTR 1 cut(s) 289
BsaJI CCNNGG 4 cut(s) 667, 720, 761, 1050
BsaWI WCCGGW 2 cut(s) 137, 879
Bsc4I CCNNNNNNNGG 4 cut(s) 224, 235, 749, 1069
Bse1I ACTGG 3 cut(s) 18, 896, 1121
Bse3DI GCAATG 1 cut(s) 481
BseBI CCWGG 1 cut(s) 669
BseCI ATCGAT 1 cut(s) 104
BseDI CCNNGG 4 cut(s) 667, 720, 761, 1050
BseGI GGATG 2 cut(s) 214, 692
BseLI CCNNNNNNNGG 4 cut(s) 224, 235, 749, 1069
BseMI GCAATG 1 cut(s) 481
BseNI ACTGG 3 cut(s) 18, 896, 1121
BseRI GAGGAG 1 cut(s) 1005
BseX3I CGGCCG 2 cut(s) 226, 241
BseXI GCAGC 1 cut(s) 916
Bsh1236I CGCG 3 cut(s) 344, 698, 722
Bsh1285I CGRYCG 2 cut(s) 229, 244
BshFI GGCC 3 cut(s) 228, 243, 1055
BshNI GGYRCC 1 cut(s) 1091
BshVI ATCGAT 1 cut(s) 104
BsiEI CGRYCG 2 cut(s) 229, 244
BsiSI CCGG 3 cut(s) 138, 225, 880
BsiWI CGTACG 1 cut(s) 285
BslFI GGGAC 3 cut(s) 291, 650, 1046
BslI CCNNNNNNNGG 4 cut(s) 224, 235, 749, 1069
BsmAI GTCTC 2 cut(s) 251, 495
BsmBI CGTCTC 1 cut(s) 251
BsmFI GGGAC 3 cut(s) 291, 650, 1046
BsnI GGCC 3 cut(s) 228, 243, 1055
Bsp143I GATC 5 cut(s) 105, 305, 325, 367, 835
BspACI CCGC 6 cut(s) 183, 344, 696, 720, 722, 939
BspANI GGCC 3 cut(s) 228, 243, 1055
BspDI ATCGAT 1 cut(s) 104
BspFNI CGCG 3 cut(s) 344, 698, 722
BspLI GGNNCC 3 cut(s) 307, 666, 1093
BspMI ACCTGC 1 cut(s) 1114
BspPI GGATC 3 cut(s) 300, 313, 375
BspT107I GGYRCC 1 cut(s) 1091
BsrDI GCAATG 1 cut(s) 481
BsrI ACTGG 3 cut(s) 18, 896, 1121
BssECI CCNNGG 4 cut(s) 667, 720, 761, 1050
BssMI GATC 5 cut(s) 105, 305, 325, 367, 835
Bst2UI CCWGG 1 cut(s) 669
Bst4CI ACNGT 3 cut(s) 285, 1037, 1091
BstAPI GCANNNNNTGC 1 cut(s) 1106
BstBAI YACGTR 1 cut(s) 289
BstDSI CCRYGG 2 cut(s) 720, 761
BstENI CCTNNNNNAGG 1 cut(s) 747
BstF5I GGATG 2 cut(s) 214, 692
BstFNI CGCG 3 cut(s) 344, 698, 722
BstKTI GATC 5 cut(s) 108, 308, 328, 370, 838
BstMAI GTCTC 2 cut(s) 251, 495
BstMBI GATC 5 cut(s) 105, 305, 325, 367, 835
BstMCI CGRYCG 2 cut(s) 229, 244
BstMWI GCNNNNNNNGC 1 cut(s) 1106
BstNI CCWGG 1 cut(s) 669
BstPAI GACNNNNGTC 1 cut(s) 243
BstSCI CCNGG 1 cut(s) 667
BstSNI TACGTA 1 cut(s) 289
BstUI CGCG 3 cut(s) 344, 698, 722
BstV1I GCAGC 1 cut(s) 916
BstV2I GAAGAC 2 cut(s) 468, 738
BstX2I RGATCY 1 cut(s) 305
BstYI RGATCY 1 cut(s) 305
BstZI CGGCCG 2 cut(s) 226, 241
Bsu15I ATCGAT 1 cut(s) 104
BsuI GTATCC 1 cut(s) 767
BsuRI GGCC 3 cut(s) 228, 243, 1055
BsuTUI ATCGAT 1 cut(s) 104
BtgI CCRYGG 2 cut(s) 720, 761
BtgZI GCGATG 1 cut(s) 112
BtsCI GGATG 2 cut(s) 214, 692
BtsIMutI CAGTG 2 cut(s) 11, 1146
BveI ACCTGC 1 cut(s) 1114
Cfr13I GGNCC 2 cut(s) 664, 1119
Cfr42I CCGCGG 1 cut(s) 723
ClaI ATCGAT 1 cut(s) 104
Csp6I GTAC 5 cut(s) 49, 286, 290, 784, 1092
CspCI CAANNNNNGTGG 2 cut(s) 46, 81
CviAII CATG 1 cut(s) 839
CviQI GTAC 5 cut(s) 49, 286, 290, 784, 1092
DpnI GATC 5 cut(s) 107, 307, 327, 369, 837
DpnII GATC 5 cut(s) 105, 305, 325, 367, 835
DraI TTTAAA 1 cut(s) 100
EaeI YGGCCR 2 cut(s) 226, 241
EagI CGGCCG 2 cut(s) 226, 241
EciI GGCGGA 1 cut(s) 172
EclXI CGGCCG 2 cut(s) 226, 241
Eco105I TACGTA 1 cut(s) 289
Eco47I GGWCC 2 cut(s) 664, 1119
Eco52I CGGCCG 2 cut(s) 226, 241
EcoNI CCTNNNNNAGG 1 cut(s) 747
EcoRI GAATTC 2 cut(s) 269, 773
EcoRII CCWGG 1 cut(s) 667
EcoT22I ATGCAT 1 cut(s) 1102
Esp3I CGTCTC 1 cut(s) 251
FaeI CATG 1 cut(s) 842
FaqI GGGAC 3 cut(s) 291, 650, 1046
FatI CATG 1 cut(s) 838
FauI CCCGC 1 cut(s) 703
FbaI TGATCA 1 cut(s) 325
Fnu4HI GCNGC 2 cut(s) 723, 930
FokI GGATG 2 cut(s) 201, 699
Fsp4HI GCNGC 2 cut(s) 723, 930
FspBI CTAG 1 cut(s) 599
GluI GCNGC 2 cut(s) 723, 930
GsuI CTGGAG 1 cut(s) 35
HaeIII GGCC 3 cut(s) 228, 243, 1055
HapII CCGG 3 cut(s) 138, 225, 880
Hin1II CATG 1 cut(s) 842
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HindIII AAGCTT 3 cut(s) 20, 31, 205
HinfI GANTC 7 cut(s) 80, 122, 398, 503, 819, 942, 1136
HpaI GTTAAC 1 cut(s) 166
HpaII CCGG 3 cut(s) 138, 225, 880
HphI GGTGA 4 cut(s) 131, 400, 533, 1095
Hpy166II GTNNAC 2 cut(s) 166, 983
Hpy188I TCNGA 9 cut(s) 79, 160, 238, 403, 717, 779, 947, 1018, 1135
Hpy188III TCNNGA 3 cut(s) 84, 802, 890
Hpy8I GTNNAC 2 cut(s) 166, 983
HpyAV CCTTC 3 cut(s) 182, 554, 1023
HpyCH4III ACNGT 3 cut(s) 285, 1037, 1091
HpyCH4IV ACGT 3 cut(s) 288, 315, 656
HpyCH4V TGCA 5 cut(s) 356, 362, 468, 486, 1100
HpyF10VI GCNNNNNNNGC 1 cut(s) 1106
HpySE526I ACGT 3 cut(s) 288, 315, 656
Hsp92II CATG 1 cut(s) 842
KpnI GGTACC 1 cut(s) 1095
Ksp22I TGATCA 1 cut(s) 325
KspAI GTTAAC 1 cut(s) 166
KspI CCGCGG 1 cut(s) 723
Kzo9I GATC 5 cut(s) 105, 305, 325, 367, 835
Lsp1109I GCAGC 1 cut(s) 916
LweI GCATC 3 cut(s) 483, 677, 1109
MaeI CTAG 1 cut(s) 599
MaeII ACGT 3 cut(s) 288, 315, 656
MaeIII GTNAC 1 cut(s) 230
MalI GATC 5 cut(s) 107, 307, 327, 369, 837
MboI GATC 5 cut(s) 105, 305, 325, 367, 835
MboII GAAGA 8 cut(s) 182, 202, 433, 468, 609, 740, 743, 1078
MflI RGATCY 1 cut(s) 305
MluCI AATT 7 cut(s) 142, 269, 337, 393, 544, 773, 1027
MlyI GAGTC 3 cut(s) 497, 813, 951
MmeI TCCRAC 1 cut(s) 925
MnlI CCTC 8 cut(s) 186, 228, 232, 481, 606, 941, 983, 1045
Mph1103I ATGCAT 1 cut(s) 1102
MseI TTAA 7 cut(s) 99, 165, 422, 435, 912, 1046, 1168
MslI CAYNNNNRTG 1 cut(s) 685
MspA1I CMGCKG 1 cut(s) 722
MspI CCGG 3 cut(s) 138, 225, 880
MspR9I CCNGG 1 cut(s) 669
MvaI CCWGG 1 cut(s) 669
MvnI CGCG 3 cut(s) 344, 698, 722
MwoI GCNNNNNNNGC 1 cut(s) 1106
NdeII GATC 5 cut(s) 105, 305, 325, 367, 835
NlaIII CATG 1 cut(s) 842
NlaIV GGNNCC 3 cut(s) 307, 666, 1093
NsiI ATGCAT 1 cut(s) 1102
PaqCI CACCTGC 1 cut(s) 1114
PcsI WCGNNNNNNNCGW 1 cut(s) 91
PfeI GAWTC 4 cut(s) 80, 122, 398, 1136
Pfl23II CGTACG 1 cut(s) 285
PflMI CCANNNNNTGG 1 cut(s) 1069
PkrI GCNGC 2 cut(s) 724, 931
PleI GAGTC 3 cut(s) 497, 813, 950
PpsI GAGTC 3 cut(s) 497, 813, 950
Ppu21I YACGTR 1 cut(s) 289
PshAI GACNNNNGTC 1 cut(s) 243
Psp1406I AACGTT 1 cut(s) 656
Psp6I CCWGG 1 cut(s) 667
PspGI CCWGG 1 cut(s) 667
PspLI CGTACG 1 cut(s) 285
PspN4I GGNNCC 3 cut(s) 307, 666, 1093
PspPI GGNCC 2 cut(s) 664, 1119
PsuI RGATCY 1 cut(s) 305
RsaI GTAC 5 cut(s) 50, 287, 291, 785, 1093
RsaNI GTAC 5 cut(s) 49, 286, 290, 784, 1092
RseI CAYNNNNRTG 1 cut(s) 685
SacII CCGCGG 1 cut(s) 723
SaqAI TTAA 7 cut(s) 99, 165, 422, 435, 912, 1046, 1168
SatI GCNGC 2 cut(s) 723, 930
Sau3AI GATC 5 cut(s) 105, 305, 325, 367, 835
Sau96I GGNCC 2 cut(s) 664, 1119
SchI GAGTC 3 cut(s) 497, 813, 951
ScrFI CCNGG 1 cut(s) 669
SfaNI GCATC 3 cut(s) 483, 677, 1109
Sfr303I CCGCGG 1 cut(s) 723
SgrBI CCGCGG 1 cut(s) 723
SinI GGWCC 2 cut(s) 664, 1119
SmiMI CAYNNNNRTG 1 cut(s) 685
SmlI CTYRAG 2 cut(s) 800, 1074
SmoI CTYRAG 2 cut(s) 800, 1074
SnaBI TACGTA 1 cut(s) 289
Sse9I AATT 7 cut(s) 142, 269, 337, 393, 544, 773, 1027
SsiI CCGC 6 cut(s) 183, 344, 696, 720, 722, 939
SspMI CTAG 1 cut(s) 599
StyD4I CCNGG 1 cut(s) 667
TaaI ACNGT 3 cut(s) 285, 1037, 1091
TaiI ACGT 3 cut(s) 291, 318, 659
TaqI TCGA 4 cut(s) 85, 104, 255, 1113
TasI AATT 7 cut(s) 142, 269, 337, 393, 544, 773, 1027
TatI WGTACW 1 cut(s) 48
TauI GCSGC 1 cut(s) 725
TfiI GAWTC 4 cut(s) 80, 122, 398, 1136
Tru1I TTAA 7 cut(s) 99, 165, 422, 435, 912, 1046, 1168
Tru9I TTAA 7 cut(s) 99, 165, 422, 435, 912, 1046, 1168
TscAI CASTG 2 cut(s) 18, 1146
TseI GCWGC 1 cut(s) 929
TspDTI ATGAA 3 cut(s) 63, 591, 786
TspRI CASTG 2 cut(s) 18, 1146
Van91I CCANNNNNTGG 1 cut(s) 1069
VpaK11BI GGWCC 2 cut(s) 664, 1119
XagI CCTNNNNNAGG 1 cut(s) 747
XapI RAATTY 2 cut(s) 269, 773
XspI CTAG 1 cut(s) 599
Zsp2I ATGCAT 1 cut(s) 1102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.