Rh5BG075200

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001486
Physical Location & Seq
Forward (+)
6328469 .. 6334046
5578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG075200.1

Sequence Viewer

Length: 939 bp
ATGGGTTCATCTGCTTCACCATATTTGATCAACTTCCTCCTCATCTTACTATCAATCATCATCATCCCAAAAGCGCTTGGGTGCTACAGTGCAATCTTCAGCTTTGGTGATTCGATTACTGATACTGGCAACTTATATATCAGCTCCAAGAACAGTTCTTTCCACTTCTTCTTCCCTCCATATGGGGAGACCTACTTTCATCGCCCCACCGGACGATGCTCCGATGGTCGCTTGATCATAGACTTCATTGCTGAGTATCTGGGACTTCCACATGTTAGACCTTATCTTGAACTCAAGAGCAATGCAAGTGCTCAAAACTTCAAAGGGGCAGTGAATTTTGCGATGGTAGGAGCCACGGCGCTTGATGCGGCATTTCTAGCAGCAATGGGAGATCATAATGATTATACAAACAGCTCTCTAGGAACTCAACTGGATTGGTTTAAAGAAATGCTGCCTTCTTTATGCAATACATCTTCAAATTGCAATAAACTGCTCTCCAGCTCCCTAGTTCTTGTGGGAGAGATTGGAGGCAACGATTACAACGATGCGTTGTTGGCAGGGAAAAGTATAGAACAGGTTCAAACATATGTACCATTAGTAATAGAAGAAATCTCTACAACAATCAAAGATTTATTGGGAGAACAGTCGAAGCATGCTGTGGTGGGGGAGGACCATACAATTACAACTCATCGGCACAATGTGGCTCTCTGGAGGCAAGTGCTTGTGAAAACCCTGCACAATTTATCAACTGGGATGGTACGTACAGAGGAGGACTTAACTGAAGCAATTGCGAAAGCAACAGAGGAGCACAAGGATAGTTTATGCTTCATCGAAGTTTTTGTGCACAAAGATGACACTAGCAAAGAGCTAATGGAATGGGGATCCCGAGTTTCAGCTGCCAACAGCCGACCTCCAAATCCAGTAGCGGACCTGAGCTGA

Protein Analysis

312

Amino Acids

34.25

Weight (kDa)

5.32

Isoelectric Point (pI)

33.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 32 - 223 6.4e-16 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 368, 926
AclWI GGATC 2 cut(s) 876, 889
AcsI RAATTY 1 cut(s) 334
AcuI CTGAAG 2 cut(s) 82, 801
AdeI CACNNNGTG 1 cut(s) 700
AfaI GTAC 3 cut(s) 591, 759, 763
AfeI AGCGCT 1 cut(s) 75
AfiI CCNNNNNNNGG 1 cut(s) 182
AflIII ACRYGT 1 cut(s) 271
AgsI TTSAA 4 cut(s) 290, 322, 477, 581
AluBI AGCT 7 cut(s) 102, 144, 414, 501, 868, 896, 936
AluI AGCT 7 cut(s) 102, 144, 414, 501, 868, 896, 936
Alw21I GWGCWC 3 cut(s) 313, 810, 846
Alw26I GTCTC 1 cut(s) 182
Alw44I GTGCAC 1 cut(s) 842
AlwI GGATC 2 cut(s) 876, 889
Ama87I CYCGRG 1 cut(s) 885
Aor51HI AGCGCT 1 cut(s) 75
ApaLI GTGCAC 1 cut(s) 842
ApeKI GCWGC 3 cut(s) 380, 451, 896
ApoI RAATTY 1 cut(s) 334
Asp700I GAANNNNTTC 1 cut(s) 576
AspLEI GCGC 2 cut(s) 76, 361
AspS9I GGNCC 2 cut(s) 670, 928
AsuHPI GGTGA 2 cut(s) 9, 119
AvaI CYCGRG 1 cut(s) 885
AvaII GGWCC 2 cut(s) 670, 928
BaeGI GKGCMC 1 cut(s) 846
BaeI ACNNNNGTAYC 2 cut(s) 573, 606
BamHI GGATCC 1 cut(s) 881
Bbv12I GWGCWC 3 cut(s) 313, 810, 846
BbvI GCAGC 3 cut(s) 392, 438, 883
BccI CCATC 3 cut(s) 218, 337, 748
BceAI ACGGC 1 cut(s) 372
BclI TGATCA 2 cut(s) 27, 234
BcoDI GTCTC 1 cut(s) 182
BfaI CTAG 4 cut(s) 377, 419, 506, 858
BfmI CTRYAG 1 cut(s) 85
BfoI RGCGCY 2 cut(s) 77, 362
BisI GCNGC 4 cut(s) 369, 381, 452, 897
BlsI GCNGC 4 cut(s) 370, 382, 453, 898
Bme18I GGWCC 2 cut(s) 670, 928
BmeT110I CYCGRG 1 cut(s) 885
BmgT120I GGNCC 2 cut(s) 670, 928
BmiI GGNNCC 2 cut(s) 352, 883
BmrI ACTGGG 1 cut(s) 759
BmsI GCATC 3 cut(s) 206, 355, 535
BmuI ACTGGG 1 cut(s) 759
BpmI CTGGAG 2 cut(s) 481, 730
Bpu10I CCTNAGC 1 cut(s) 932
BpuEI CTTGAG 1 cut(s) 278
BsaAI YACGTR 1 cut(s) 761
BsaI GGTCTC 1 cut(s) 182
BsaJI CCNNGG 1 cut(s) 354
BsaWI WCCGGW 1 cut(s) 209
Bsc4I CCNNNNNNNGG 1 cut(s) 182
Bse1I ACTGG 4 cut(s) 130, 435, 754, 920
Bse3DI GCAATG 3 cut(s) 246, 307, 390
BseDI CCNNGG 1 cut(s) 354
BseGI GGATG 2 cut(s) 63, 759
BseLI CCNNNNNNNGG 1 cut(s) 182
BseMI GCAATG 3 cut(s) 246, 307, 390
BseMII CTCAG 2 cut(s) 243, 923
BseNI ACTGG 4 cut(s) 130, 435, 754, 920
BseRI GAGGAG 3 cut(s) 29, 782, 818
BseSI GKGCMC 1 cut(s) 846
BseXI GCAGC 3 cut(s) 392, 438, 883
BsgI GTGCAG 1 cut(s) 719
BsiHKAI GWGCWC 3 cut(s) 313, 810, 846
BsiHKCI CYCGRG 1 cut(s) 885
BsiSI CCGG 1 cut(s) 210
BslFI GGGAC 1 cut(s) 276
BslI CCNNNNNNNGG 1 cut(s) 182
BsmAI GTCTC 1 cut(s) 182
BsmFI GGGAC 1 cut(s) 276
Bso31I GGTCTC 1 cut(s) 182
BsoBI CYCGRG 1 cut(s) 885
Bsp1286I GDGCHC 3 cut(s) 313, 810, 846
Bsp143I GATC 4 cut(s) 27, 234, 391, 881
BspACI CCGC 2 cut(s) 368, 926
BspCNI CTCAG 2 cut(s) 244, 924
BspLI GGNNCC 2 cut(s) 352, 883
BspPI GGATC 2 cut(s) 876, 889
BspTNI GGTCTC 1 cut(s) 182
BsrDI GCAATG 3 cut(s) 246, 307, 390
BsrI ACTGG 4 cut(s) 130, 435, 754, 920
BssECI CCNNGG 1 cut(s) 354
BssMI GATC 4 cut(s) 27, 234, 391, 881
Bst4CI ACNGT 3 cut(s) 89, 155, 645
BstBAI YACGTR 1 cut(s) 761
BstC8I GCNNGC 1 cut(s) 654
BstDEI CTNAG 2 cut(s) 252, 932
BstDSI CCRYGG 1 cut(s) 354
BstF5I GGATG 2 cut(s) 63, 759
BstH2I RGCGCY 2 cut(s) 77, 362
BstHHI GCGC 2 cut(s) 76, 361
BstKTI GATC 4 cut(s) 30, 237, 394, 884
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 4 cut(s) 27, 234, 391, 881
BstMWI GCNNNNNNNGC 3 cut(s) 365, 377, 554
BstNSI RCATGY 2 cut(s) 275, 656
BstSFI CTRYAG 1 cut(s) 85
BstSLI GKGCMC 1 cut(s) 846
BstSNI TACGTA 1 cut(s) 761
BstV1I GCAGC 3 cut(s) 392, 438, 883
BstX2I RGATCY 1 cut(s) 881
BstYI RGATCY 1 cut(s) 881
BtgI CCRYGG 1 cut(s) 354
BtgZI GCGATG 2 cut(s) 185, 356
BtsCI GGATG 2 cut(s) 63, 759
BtsI GCAGTG 1 cut(s) 336
BtsIMutI CAGTG 2 cut(s) 94, 336
Cac8I GCNNGC 1 cut(s) 654
CfoI GCGC 2 cut(s) 76, 361
Cfr13I GGNCC 2 cut(s) 670, 928
Csp6I GTAC 3 cut(s) 590, 758, 762
CspCI CAANNNNNGTGG 2 cut(s) 343, 378
CviAII CATG 2 cut(s) 272, 653
CviQI GTAC 3 cut(s) 590, 758, 762
DdeI CTNAG 2 cut(s) 252, 932
DpnI GATC 4 cut(s) 29, 236, 393, 883
DpnII GATC 4 cut(s) 27, 234, 391, 881
DraI TTTAAA 1 cut(s) 442
DraIII CACNNNGTG 1 cut(s) 700
Eco105I TACGTA 1 cut(s) 761
Eco31I GGTCTC 1 cut(s) 182
Eco47I GGWCC 2 cut(s) 670, 928
Eco47III AGCGCT 1 cut(s) 75
Eco57I CTGAAG 2 cut(s) 82, 801
Eco88I CYCGRG 1 cut(s) 885
FaeI CATG 2 cut(s) 275, 656
FaqI GGGAC 1 cut(s) 276
FatI CATG 2 cut(s) 271, 652
FauNDI CATATG 2 cut(s) 181, 586
FbaI TGATCA 2 cut(s) 27, 234
Fnu4HI GCNGC 4 cut(s) 369, 381, 452, 897
FokI GGATG 2 cut(s) 50, 766
Fsp4HI GCNGC 4 cut(s) 369, 381, 452, 897
FspBI CTAG 4 cut(s) 377, 419, 506, 858
GlaI GCGC 2 cut(s) 75, 360
GluI GCNGC 4 cut(s) 369, 381, 452, 897
GsuI CTGGAG 2 cut(s) 481, 730
HaeII RGCGCY 2 cut(s) 77, 362
HapII CCGG 1 cut(s) 210
HhaI GCGC 2 cut(s) 76, 361
Hin1II CATG 2 cut(s) 275, 656
Hin6I GCGC 2 cut(s) 74, 359
HinP1I GCGC 2 cut(s) 74, 359
HinfI GANTC 1 cut(s) 110
HpaII CCGG 1 cut(s) 210
HphI GGTGA 2 cut(s) 9, 119
Hpy166II GTNNAC 1 cut(s) 844
Hpy188I TCNGA 1 cut(s) 223
Hpy188III TCNNGA 4 cut(s) 287, 295, 709, 885
Hpy8I GTNNAC 1 cut(s) 844
HpyAV CCTTC 1 cut(s) 465
HpyCH4III ACNGT 3 cut(s) 89, 155, 645
HpyCH4IV ACGT 1 cut(s) 760
HpyCH4V TGCA 6 cut(s) 92, 305, 465, 483, 736, 844
HpyF10VI GCNNNNNNNGC 3 cut(s) 365, 377, 554
HpyF3I CTNAG 2 cut(s) 252, 932
HpySE526I ACGT 1 cut(s) 760
Hsp92II CATG 2 cut(s) 275, 656
HspAI GCGC 2 cut(s) 74, 359
Ksp22I TGATCA 2 cut(s) 27, 234
Kzo9I GATC 4 cut(s) 27, 234, 391, 881
LmnI GCTCC 5 cut(s) 149, 224, 350, 506, 805
Lsp1109I GCAGC 3 cut(s) 392, 438, 883
LweI GCATC 3 cut(s) 206, 355, 535
MaeI CTAG 4 cut(s) 377, 419, 506, 858
MaeII ACGT 1 cut(s) 760
MalI GATC 4 cut(s) 29, 236, 393, 883
MboI GATC 4 cut(s) 27, 234, 391, 881
MboII GAAGA 5 cut(s) 88, 160, 163, 465, 617
MfeI CAATTG 1 cut(s) 786
MflI RGATCY 1 cut(s) 881
MhlI GDGCHC 3 cut(s) 313, 810, 846
MluCI AATT 5 cut(s) 334, 478, 678, 739, 786
MroXI GAANNNNTTC 1 cut(s) 576
MseI TTAA 2 cut(s) 441, 776
MslI CAYNNNNRTG 1 cut(s) 849
MspA1I CMGCKG 1 cut(s) 896
MspI CCGG 1 cut(s) 210
MunI CAATTG 1 cut(s) 786
MwoI GCNNNNNNNGC 3 cut(s) 365, 377, 554
NdeI CATATG 2 cut(s) 181, 586
NdeII GATC 4 cut(s) 27, 234, 391, 881
NlaIII CATG 2 cut(s) 275, 656
NlaIV GGNNCC 2 cut(s) 352, 883
NspI RCATGY 2 cut(s) 275, 656
PaeI GCATGC 1 cut(s) 656
PciI ACATGT 1 cut(s) 271
PcsI WCGNNNNNNNCGW 1 cut(s) 540
PdmI GAANNNNTTC 1 cut(s) 576
PfeI GAWTC 1 cut(s) 110
PkrI GCNGC 4 cut(s) 370, 382, 453, 898
Ppu21I YACGTR 1 cut(s) 761
PscI ACATGT 1 cut(s) 271
PspN4I GGNNCC 2 cut(s) 352, 883
PspPI GGNCC 2 cut(s) 670, 928
PsuI RGATCY 1 cut(s) 881
PvuII CAGCTG 1 cut(s) 896
RsaI GTAC 3 cut(s) 591, 759, 763
RsaNI GTAC 3 cut(s) 590, 758, 762
RseI CAYNNNNRTG 1 cut(s) 849
SaqAI TTAA 2 cut(s) 441, 776
SatI GCNGC 4 cut(s) 369, 381, 452, 897
Sau3AI GATC 4 cut(s) 27, 234, 391, 881
Sau96I GGNCC 2 cut(s) 670, 928
SduI GDGCHC 3 cut(s) 313, 810, 846
SfaNI GCATC 3 cut(s) 206, 355, 535
SfcI CTRYAG 1 cut(s) 85
SinI GGWCC 2 cut(s) 670, 928
SmiMI CAYNNNNRTG 1 cut(s) 849
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
SnaBI TACGTA 1 cut(s) 761
SphI GCATGC 1 cut(s) 656
Sse9I AATT 5 cut(s) 334, 478, 678, 739, 786
SsiI CCGC 2 cut(s) 368, 926
SspMI CTAG 4 cut(s) 377, 419, 506, 858
TaaI ACNGT 3 cut(s) 89, 155, 645
TaiI ACGT 1 cut(s) 763
TaqI TCGA 3 cut(s) 113, 647, 831
TasI AATT 5 cut(s) 334, 478, 678, 739, 786
TauI GCSGC 1 cut(s) 371
TfiI GAWTC 1 cut(s) 110
Tru1I TTAA 2 cut(s) 441, 776
Tru9I TTAA 2 cut(s) 441, 776
TscAI CASTG 2 cut(s) 94, 336
TseI GCWGC 3 cut(s) 380, 451, 896
TspDTI ATGAA 3 cut(s) 188, 235, 817
TspRI CASTG 2 cut(s) 94, 336
VneI GTGCAC 1 cut(s) 842
VpaK11BI GGWCC 2 cut(s) 670, 928
XapI RAATTY 1 cut(s) 334
XceI RCATGY 2 cut(s) 275, 656
XmnI GAANNNNTTC 1 cut(s) 576
XspI CTAG 4 cut(s) 377, 419, 506, 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.