AT1G31550

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001486
Physical Location & Seq
Reverse (-)
11295446 .. 11297379
1934 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G31550.2

Sequence Viewer

Length: 1176 bp
ATGGCGTCGCTAGATTCTCATGTGTTGATGAAACTAGGTAGCCTCTTTTTATCTACTCTTTTTGTTTCTATCGTAAGCTCGGAATCGCAGTGCCGGAACTTCGAGTCGATCATCAGTTTCGGCGATTCGATTGCCGACACTGGAAACTTGCTAGGTCTCTCAGATCATAACAATCTTCCTATGAGCGCGTTTCCACCGTACGGAGAAACTTTCTTCCACCACCCAACCGGTCGTTTCTCTGACGGTCGCCTCATCATTGACTTCATCGCTGAATTTTTGGGGCTTCCTTATGTGCCTCCTTACTTCGGATCTACAAATGGAAACTTTGAGAAAGGAGTTAATTTTGCCGTAGCCTCAGCAACTGCATTGGAAAGTTCTTTTCTAGAAGAGAAAGGATACCATTGTCCTCACAACTTCAGTTTAGGAGTTCAGCTTAAGATCTTCAAGCAGAGCTTACCAAATCTATGTGGCTTACCATCAGATTGTAGAGATATGATTGGAAATGCTTTGATTCTCATGGGAGAGATTGGAGCTAATGATTACAATTTCCCATTCTTCCAACTCAGACCATTGGACGAGGTCAAAGAGCTTGTTCCGTTGGTGATTAGTACTATTTCTTCTGCGATTACGGAGTTGATTGGTATGGGAGGAAGAACATTTCTGGTGCCCGGAGGGTTCCCTCTCGGATGCTCAGTAGCATTTTTGACATTACACCAAACATCAAACATGGAAGAATATGATCCTTTAACAGGATGTTTGAAATGGCTGAACAAGTTTGGAGAATACCACAGTGAGCAGCTTCAGGAAGAACTCAACAGACTCAGGAAGCTTAACCCTCACGTCAACATCATATATGCTGACTACTACAATGCTTCATTGCGCCTTGGCCGAGAACCAAGATTCATAAACAGACACTTGTCAGCTTGTTGCGGTGTAGGAGGGCCGTACAACTTCAACTTAAGTAGAAGTTGTGGGAGTGTAGGAGTGGAAGCTTGTAGTGATCCTTCAAAGTATGTGGCCTGGGATGGCCTTCATATGACTGAGGCTGCACACAAATCGATGGCTGATGGATTAGTCAAAGGACCATATGCGATTCCTCCTTTCGATTGGTCATGTCTCAGCTCAATGATTAAGAAGAAGGAAGTCGTTGGAAACACAATATTCTTTGATGAATAG

Protein Analysis

391

Amino Acids

43.1

Weight (kDa)

5.46

Isoelectric Point (pI)

41.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 37 - 360 1.5e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 664
AccII CGCG 1 cut(s) 188
AciI CCGC 1 cut(s) 930
AclWI GGATC 3 cut(s) 316, 734, 995
AcoI YGGCCR 1 cut(s) 886
AcsI RAATTY 1 cut(s) 272
AcuI CTGAAG 2 cut(s) 400, 785
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 200, 610, 947
AfiI CCNNNNNNNGG 3 cut(s) 200, 305, 749
AflII CTTAAG 2 cut(s) 434, 958
AgeI ACCGGT 1 cut(s) 227
AgsI TTSAA 4 cut(s) 445, 760, 955, 1008
AjiI CACGTC 1 cut(s) 841
AjnI CCWGG 1 cut(s) 1019
Alw26I GTCTC 2 cut(s) 161, 1121
AlwI GGATC 3 cut(s) 316, 734, 995
AlwNI CAGNNNCTG 1 cut(s) 362
AoxI GGCC 4 cut(s) 886, 941, 1017, 1027
ApeKI GCWGC 2 cut(s) 796, 1046
ApoI RAATTY 1 cut(s) 272
AsiGI ACCGGT 1 cut(s) 227
AspLEI GCGC 2 cut(s) 188, 882
AspS9I GGNCC 2 cut(s) 941, 1082
AsuC2I CCSGG 1 cut(s) 669
AsuHPI GGTGA 1 cut(s) 613
AvaII GGWCC 1 cut(s) 1082
BaeGI GKGCMC 1 cut(s) 669
BaeI ACNNNNGTAYC 2 cut(s) 388, 421
BanI GGYRCC 1 cut(s) 664
BarI GAAGNNNNNNTAC 4 cut(s) 601, 633, 988, 1020
BbvCI CCTCAGC 1 cut(s) 355
BbvI GCAGC 2 cut(s) 808, 1033
BccI CCATC 4 cut(s) 484, 1019, 1054, 1061
BceAI ACGGC 2 cut(s) 332, 928
BcgI CGANNNNNNTGC 4 cut(s) 113, 147, 1038, 1072
BciT130I CCWGG 1 cut(s) 1021
BciVI GTATCC 1 cut(s) 389
BcnI CCSGG 1 cut(s) 669
BcoDI GTCTC 2 cut(s) 161, 1121
BfaI CTAG 4 cut(s) 11, 35, 152, 383
BfrI CTTAAG 2 cut(s) 434, 958
BfuI GTATCC 1 cut(s) 389
BglII AGATCT 1 cut(s) 438
BisI GCNGC 2 cut(s) 797, 1047
BlsI GCNGC 2 cut(s) 798, 1048
BmcAI AGTACT 1 cut(s) 610
Bme1390I CCNGG 2 cut(s) 669, 1021
Bme18I GGWCC 1 cut(s) 1082
BmgBI CACGTC 1 cut(s) 841
BmgT120I GGNCC 2 cut(s) 941, 1082
BmiI GGNNCC 2 cut(s) 666, 677
BmrFI CCNGG 2 cut(s) 669, 1021
BmsI GCATC 1 cut(s) 677
BoxI GACNNNNGTC 1 cut(s) 916
Bpu10I CCTNAGC 1 cut(s) 355
BpuMI CCSGG 1 cut(s) 669
Bsa29I ATCGAT 1 cut(s) 1058
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 161
BsaJI CCNNGG 2 cut(s) 883, 1020
BsaWI WCCGGW 1 cut(s) 227
Bsc4I CCNNNNNNNGG 3 cut(s) 200, 305, 749
Bse118I RCCGGY 1 cut(s) 227
Bse1I ACTGG 1 cut(s) 145
Bse3DI GCAATG 1 cut(s) 875
BseBI CCWGG 1 cut(s) 1021
BseCI ATCGAT 1 cut(s) 1058
BseDI CCNNGG 2 cut(s) 883, 1020
BseGI GGATG 3 cut(s) 692, 758, 1030
BseLI CCNNNNNNNGG 3 cut(s) 200, 305, 749
BseMI GCAATG 1 cut(s) 875
BseMII CTCAG 7 cut(s) 174, 369, 577, 705, 835, 1032, 1132
BseNI ACTGG 1 cut(s) 145
BseSI GKGCMC 1 cut(s) 669
BseXI GCAGC 2 cut(s) 808, 1033
BsgI GTGCAG 1 cut(s) 1032
Bsh1236I CGCG 1 cut(s) 188
Bsh1285I CGRYCG 2 cut(s) 232, 247
BshFI GGCC 4 cut(s) 888, 943, 1019, 1029
BshNI GGYRCC 1 cut(s) 664
BshTI ACCGGT 1 cut(s) 227
BshVI ATCGAT 1 cut(s) 1058
BsiEI CGRYCG 2 cut(s) 232, 247
BsiSI CCGG 3 cut(s) 94, 228, 669
BsiWI CGTACG 1 cut(s) 198
BslI CCNNNNNNNGG 3 cut(s) 200, 305, 749
BsmAI GTCTC 2 cut(s) 161, 1121
BsnI GGCC 4 cut(s) 888, 943, 1019, 1029
Bso31I GGTCTC 1 cut(s) 161
Bsp1286I GDGCHC 1 cut(s) 669
Bsp143I GATC 6 cut(s) 108, 163, 308, 438, 739, 1000
BspACI CCGC 1 cut(s) 930
BspANI GGCC 4 cut(s) 888, 943, 1019, 1029
BspCNI CTCAG 7 cut(s) 173, 368, 576, 704, 834, 1033, 1131
BspDI ATCGAT 1 cut(s) 1058
BspFNI CGCG 1 cut(s) 188
BspLI GGNNCC 2 cut(s) 666, 677
BspPI GGATC 3 cut(s) 316, 734, 995
BspT107I GGYRCC 1 cut(s) 664
BspTI CTTAAG 2 cut(s) 434, 958
BspTNI GGTCTC 1 cut(s) 161
BsrDI GCAATG 1 cut(s) 875
BsrFI RCCGGY 1 cut(s) 227
BsrI ACTGG 1 cut(s) 145
BssAI RCCGGY 1 cut(s) 227
BssECI CCNNGG 2 cut(s) 883, 1020
BssMI GATC 6 cut(s) 108, 163, 308, 438, 739, 1000
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 883
Bst2UI CCWGG 1 cut(s) 1021
Bst4CI ACNGT 3 cut(s) 198, 245, 791
Bst6I CTCTTC 1 cut(s) 381
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 2 cut(s) 434, 958
BstDEI CTNAG 7 cut(s) 160, 355, 563, 691, 821, 1041, 1118
BstENI CCTNNNNNAGG 1 cut(s) 747
BstF5I GGATG 3 cut(s) 692, 758, 1030
BstFNI CGCG 1 cut(s) 188
BstHHI GCGC 2 cut(s) 188, 882
BstKTI GATC 6 cut(s) 111, 166, 311, 441, 742, 1003
BstMAI GTCTC 2 cut(s) 161, 1121
BstMBI GATC 6 cut(s) 108, 163, 308, 438, 739, 1000
BstMCI CGRYCG 2 cut(s) 232, 247
BstNI CCWGG 1 cut(s) 1021
BstPAI GACNNNNGTC 1 cut(s) 916
BstSCI CCNGG 2 cut(s) 667, 1019
BstSLI GKGCMC 1 cut(s) 669
BstUI CGCG 1 cut(s) 188
BstV1I GCAGC 2 cut(s) 808, 1033
BstX2I RGATCY 2 cut(s) 308, 438
BstYI RGATCY 2 cut(s) 308, 438
Bsu15I ATCGAT 1 cut(s) 1058
BsuI GTATCC 1 cut(s) 389
BsuRI GGCC 4 cut(s) 888, 943, 1019, 1029
BsuTUI ATCGAT 1 cut(s) 1058
BtgZI GCGATG 1 cut(s) 250
BtrI CACGTC 1 cut(s) 841
BtsCI GGATG 3 cut(s) 692, 758, 1030
BtsI GCAGTG 1 cut(s) 95
BtsIMutI CAGTG 3 cut(s) 95, 138, 796
CaiI CAGNNNCTG 1 cut(s) 362
CfoI GCGC 2 cut(s) 188, 882
Cfr10I RCCGGY 1 cut(s) 227
Cfr13I GGNCC 2 cut(s) 941, 1082
ClaI ATCGAT 1 cut(s) 1058
Csp6I GTAC 3 cut(s) 199, 609, 946
CspAI ACCGGT 1 cut(s) 227
CspCI CAANNNNNGTGG 2 cut(s) 996, 1031
CviAII CATG 4 cut(s) 20, 517, 727, 1113
CviQI GTAC 3 cut(s) 199, 609, 946
DdeI CTNAG 7 cut(s) 160, 355, 563, 691, 821, 1041, 1118
DpnI GATC 6 cut(s) 110, 165, 310, 440, 741, 1002
DpnII GATC 6 cut(s) 108, 163, 308, 438, 739, 1000
EaeI YGGCCR 1 cut(s) 886
Eam1104I CTCTTC 1 cut(s) 381
EarI CTCTTC 1 cut(s) 381
Eco130I CCWWGG 1 cut(s) 883
Eco31I GGTCTC 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 1082
Eco57I CTGAAG 2 cut(s) 400, 785
EcoNI CCTNNNNNAGG 1 cut(s) 747
EcoRII CCWGG 1 cut(s) 1019
EcoT14I CCWWGG 1 cut(s) 883
ErhI CCWWGG 1 cut(s) 883
FaeI CATG 4 cut(s) 23, 520, 730, 1116
FalI AAGNNNNNCTT 2 cut(s) 437, 469
FatI CATG 4 cut(s) 19, 516, 726, 1112
FauNDI CATATG 2 cut(s) 1035, 1087
Fnu4HI GCNGC 2 cut(s) 797, 1047
FokI GGATG 3 cut(s) 699, 765, 1037
Fsp4HI GCNGC 2 cut(s) 797, 1047
FspBI CTAG 4 cut(s) 11, 35, 152, 383
GlaI GCGC 2 cut(s) 187, 881
GluI GCNGC 2 cut(s) 797, 1047
HaeIII GGCC 4 cut(s) 888, 943, 1019, 1029
HapII CCGG 3 cut(s) 94, 228, 669
HhaI GCGC 2 cut(s) 188, 882
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 4 cut(s) 23, 520, 730, 1116
Hin6I GCGC 2 cut(s) 186, 880
HinP1I GCGC 2 cut(s) 186, 880
HincII GTYRAC 1 cut(s) 844
HindII GTYRAC 1 cut(s) 844
HindIII AAGCTT 2 cut(s) 827, 990
HinfI GANTC 8 cut(s) 14, 83, 104, 125, 511, 819, 900, 1093
HpaII CCGG 3 cut(s) 94, 228, 669
HphI GGTGA 1 cut(s) 613
Hpy166II GTNNAC 1 cut(s) 844
Hpy188I TCNGA 7 cut(s) 82, 163, 241, 308, 481, 566, 686
Hpy188III TCNNGA 3 cut(s) 383, 803, 823
Hpy8I GTNNAC 1 cut(s) 844
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 3 cut(s) 1014, 1040, 1132
HpyCH4III ACNGT 3 cut(s) 198, 245, 791
HpyCH4IV ACGT 1 cut(s) 840
HpyCH4V TGCA 2 cut(s) 365, 1049
HpyF3I CTNAG 7 cut(s) 160, 355, 563, 691, 821, 1041, 1118
HpySE526I ACGT 1 cut(s) 840
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 4 cut(s) 23, 520, 730, 1116
HspAI GCGC 2 cut(s) 186, 880
Kzo9I GATC 6 cut(s) 108, 163, 308, 438, 739, 1000
LmnI GCTCC 1 cut(s) 530
Lsp1109I GCAGC 2 cut(s) 808, 1033
LweI GCATC 1 cut(s) 677
MaeI CTAG 4 cut(s) 11, 35, 152, 383
MaeII ACGT 1 cut(s) 840
MalI GATC 6 cut(s) 110, 165, 310, 440, 741, 1002
MboI GATC 6 cut(s) 108, 163, 308, 438, 739, 1000
MflI RGATCY 2 cut(s) 308, 438
MhlI GDGCHC 1 cut(s) 669
MluCI AATT 3 cut(s) 272, 340, 544
MlyI GAGTC 2 cut(s) 113, 813
MmeI TCCRAC 2 cut(s) 583, 1129
MseI TTAA 6 cut(s) 339, 435, 746, 831, 959, 1131
MspCI CTTAAG 2 cut(s) 434, 958
MspI CCGG 3 cut(s) 94, 228, 669
MspR9I CCNGG 2 cut(s) 669, 1021
MvaI CCWGG 1 cut(s) 1021
MvnI CGCG 1 cut(s) 188
NciI CCSGG 1 cut(s) 669
NdeI CATATG 2 cut(s) 1035, 1087
NdeII GATC 6 cut(s) 108, 163, 308, 438, 739, 1000
NlaIII CATG 4 cut(s) 23, 520, 730, 1116
NlaIV GGNNCC 2 cut(s) 666, 677
NmeAIII GCCGAG 1 cut(s) 914
PfeI GAWTC 6 cut(s) 14, 83, 125, 511, 900, 1093
Pfl23II CGTACG 1 cut(s) 198
PflFI GACNNNGTC 1 cut(s) 578
PinAI ACCGGT 1 cut(s) 227
PkrI GCNGC 2 cut(s) 798, 1048
PleI GAGTC 2 cut(s) 112, 813
PpsI GAGTC 2 cut(s) 112, 813
PshAI GACNNNNGTC 1 cut(s) 916
Psp6I CCWGG 1 cut(s) 1019
PspGI CCWGG 1 cut(s) 1019
PspLI CGTACG 1 cut(s) 198
PspN4I GGNNCC 2 cut(s) 666, 677
PspPI GGNCC 2 cut(s) 941, 1082
PstNI CAGNNNCTG 1 cut(s) 362
PsuI RGATCY 2 cut(s) 308, 438
PsyI GACNNNGTC 1 cut(s) 578
RsaI GTAC 3 cut(s) 200, 610, 947
RsaNI GTAC 3 cut(s) 199, 609, 946
SaqAI TTAA 6 cut(s) 339, 435, 746, 831, 959, 1131
SatI GCNGC 2 cut(s) 797, 1047
Sau3AI GATC 6 cut(s) 108, 163, 308, 438, 739, 1000
Sau96I GGNCC 2 cut(s) 941, 1082
ScaI AGTACT 1 cut(s) 610
SchI GAGTC 2 cut(s) 113, 813
ScrFI CCNGG 2 cut(s) 669, 1021
SduI GDGCHC 1 cut(s) 669
SfaNI GCATC 1 cut(s) 677
SinI GGWCC 1 cut(s) 1082
SmlI CTYRAG 2 cut(s) 434, 958
SmoI CTYRAG 2 cut(s) 434, 958
Sse9I AATT 3 cut(s) 272, 340, 544
SsiI CCGC 1 cut(s) 930
SspI AATATT 1 cut(s) 1161
SspMI CTAG 4 cut(s) 11, 35, 152, 383
StyD4I CCNGG 2 cut(s) 667, 1019
StyI CCWWGG 1 cut(s) 883
TaaI ACNGT 3 cut(s) 198, 245, 791
TaiI ACGT 1 cut(s) 843
TaqI TCGA 5 cut(s) 102, 107, 128, 1058, 1104
TasI AATT 3 cut(s) 272, 340, 544
TatI WGTACW 1 cut(s) 608
TfiI GAWTC 6 cut(s) 14, 83, 125, 511, 900, 1093
Tru1I TTAA 6 cut(s) 339, 435, 746, 831, 959, 1131
Tru9I TTAA 6 cut(s) 339, 435, 746, 831, 959, 1131
TscAI CASTG 3 cut(s) 95, 145, 796
TseI GCWGC 2 cut(s) 796, 1046
TspDTI ATGAA 5 cut(s) 44, 253, 864, 892, 1022
TspGWI ACGGA 3 cut(s) 216, 585, 644
TspRI CASTG 3 cut(s) 95, 145, 796
Tth111I GACNNNGTC 1 cut(s) 578
Vha464I CTTAAG 2 cut(s) 434, 958
VpaK11BI GGWCC 1 cut(s) 1082
XagI CCTNNNNNAGG 1 cut(s) 747
XapI RAATTY 1 cut(s) 272
XbaI TCTAGA 1 cut(s) 382
XspI CTAG 4 cut(s) 11, 35, 152, 383
ZrmI AGTACT 1 cut(s) 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.