Rroxscaffold_1G00066020

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001486
Physical Location & Seq
Reverse (-)
87560111 .. 87561776
1666 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00066020.1

Sequence Viewer

Length: 1062 bp
ATGGGTTCATCTTCTTCACCATATTTGATCAACTTCCTCCTCATCTTACTATCAATCATCATCCCAAAAGCGCTTGGGTGCTACAGTGCAATCTTCAGCTTTGGTGATTCGATTACTGATACTGGCAACTTATATATCAGCTCCAAGAACAGTTCTTTCCACTTCTTCTTCCCTCCATATGGGGAGACCTACTTTCATCGCCCCACCGGACGATGCTCCGATGGTCGCTTGATCATAGACTTCATTGCTGAGTATCTGGGACTTCCACATGTTAGACCTTATCTTGAACTCAAGAGCAATGCAAGTGCTCAAAACTTCAAAGGGGCAGTGAATTTTGCGATGGTAGGAGCCACGGCGCTTGATGCGGCATTTCTAGCAGCAATGGGAGATCATAATGATTATACAAACAGCTCTCTAGGAACTCAACTGGATTGGTTTAAAGAAATGCTGCCTTCTGTATGCAATACATCTTCAAATTGCAATAAGCTTCTCTCCAGCTCTCTAGTTCTTGTGGGAGAGATTGGAGGCAACGATTACAACGATGCATTGTTGGCAGGGAAAAGTATAGAACAGGACTTAATTGAGCTTGGGGCAATGACACTTATGGTACCTGGCAACTTTCCAATCGGTTGCCTTCCCATTTATCTTACAAAATTTCGGACTTCAGATGAAAAGCAGTACGACTCCTCGACAGGTTGTCTGAATTGGTTAAACAAGTTTGCTGAATATCACAATGACCAGCTTCAAATAGAACTAAGTCAGGTTCAAAGGCTTCACCCCAAAGTCAACATCATTTATGCAGATTATTACAATGCAGTGATGGATTTCTATCATTCTCCAGACCAATTCGGATTTATTGGGGGAACAGTCGAAGCATGCTGTGGAGGGGGAGGACCATACAATTACAACTCATCGGCACAATGTGGCTCTCTGGAGGCAAGTGCTTGTGAAAACCCTGCACAGTTTATCAACTGGGATGGTATACACTTTACTGAAGCAGCATATAGGTGGATAGCCAAAGGTTTATTGAGAGGCAAATTAATACAGCATTCCCAGTATTAG

Protein Analysis

353

Amino Acids

38.9

Weight (kDa)

5.2

Isoelectric Point (pI)

35.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 31 - 341 2.8e-25 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 607
AccB1I GGYRCC 1 cut(s) 607
AccI GTMKAC 1 cut(s) 982
AciI CCGC 1 cut(s) 365
AcsI RAATTY 2 cut(s) 331, 653
AcuI CTGAAG 3 cut(s) 79, 648, 1014
AdeI CACNNNGTG 1 cut(s) 923
AfaI GTAC 2 cut(s) 609, 680
AfeI AGCGCT 1 cut(s) 72
AfiI CCNNNNNNNGG 1 cut(s) 179
AflIII ACRYGT 1 cut(s) 268
AgsI TTSAA 5 cut(s) 287, 319, 474, 746, 767
AhdI GACNNNNNGTC 1 cut(s) 696
AjnI CCWGG 1 cut(s) 610
AluBI AGCT 7 cut(s) 99, 141, 411, 487, 498, 586, 742
AluI AGCT 7 cut(s) 99, 141, 411, 487, 498, 586, 742
Alw21I GWGCWC 1 cut(s) 310
Alw26I GTCTC 1 cut(s) 179
Aor51HI AGCGCT 1 cut(s) 72
ApeKI GCWGC 3 cut(s) 377, 448, 998
ApoI RAATTY 2 cut(s) 331, 653
AseI ATTAAT 1 cut(s) 1040
Asp718I GGTACC 1 cut(s) 607
AspLEI GCGC 2 cut(s) 73, 358
AspS9I GGNCC 1 cut(s) 893
AsuHPI GGTGA 3 cut(s) 9, 116, 767
AvaII GGWCC 1 cut(s) 893
BanI GGYRCC 1 cut(s) 607
Bbv12I GWGCWC 1 cut(s) 310
BbvI GCAGC 3 cut(s) 389, 435, 1010
BccI CCATC 4 cut(s) 215, 334, 814, 971
BceAI ACGGC 1 cut(s) 369
BciT130I CCWGG 1 cut(s) 612
BclI TGATCA 2 cut(s) 27, 231
BcoDI GTCTC 1 cut(s) 179
BfaI CTAG 3 cut(s) 374, 416, 503
BfmI CTRYAG 1 cut(s) 82
BfoI RGCGCY 2 cut(s) 74, 359
BisI GCNGC 4 cut(s) 366, 378, 449, 999
BlsI GCNGC 4 cut(s) 367, 379, 450, 1000
Bme1390I CCNGG 1 cut(s) 612
Bme18I GGWCC 1 cut(s) 893
BmeRI GACNNNNNGTC 1 cut(s) 696
BmgT120I GGNCC 1 cut(s) 893
BmiI GGNNCC 2 cut(s) 349, 609
BmrFI CCNGG 1 cut(s) 612
BmrI ACTGGG 2 cut(s) 982, 1048
BmsI GCATC 3 cut(s) 203, 352, 532
BmuI ACTGGG 2 cut(s) 982, 1048
BpmI CTGGAG 3 cut(s) 478, 822, 953
BpuEI CTTGAG 1 cut(s) 275
BsaBI GATNNNNATC 1 cut(s) 828
BsaI GGTCTC 1 cut(s) 179
BsaJI CCNNGG 1 cut(s) 351
BsaWI WCCGGW 1 cut(s) 206
Bsc4I CCNNNNNNNGG 1 cut(s) 179
Bse1I ACTGG 4 cut(s) 127, 432, 977, 1054
Bse3DI GCAATG 4 cut(s) 243, 304, 387, 600
Bse8I GATNNNNATC 1 cut(s) 828
BseBI CCWGG 1 cut(s) 612
BseDI CCNNGG 1 cut(s) 351
BseGI GGATG 2 cut(s) 60, 982
BseJI GATNNNNATC 1 cut(s) 828
BseLI CCNNNNNNNGG 1 cut(s) 179
BseMI GCAATG 4 cut(s) 243, 304, 387, 600
BseMII CTCAG 1 cut(s) 240
BseNI ACTGG 4 cut(s) 127, 432, 977, 1054
BseRI GAGGAG 2 cut(s) 29, 676
BseXI GCAGC 3 cut(s) 389, 435, 1010
BsgI GTGCAG 1 cut(s) 942
BshNI GGYRCC 1 cut(s) 607
BsiHKAI GWGCWC 1 cut(s) 310
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 1 cut(s) 273
BslI CCNNNNNNNGG 1 cut(s) 179
BsmAI GTCTC 1 cut(s) 179
BsmFI GGGAC 1 cut(s) 273
BsmI GAATGC 1 cut(s) 1048
Bso31I GGTCTC 1 cut(s) 179
Bsp1286I GDGCHC 1 cut(s) 310
Bsp143I GATC 3 cut(s) 27, 231, 388
BspACI CCGC 1 cut(s) 365
BspCNI CTCAG 1 cut(s) 241
BspLI GGNNCC 2 cut(s) 349, 609
BspT107I GGYRCC 1 cut(s) 607
BspTNI GGTCTC 1 cut(s) 179
BsrDI GCAATG 4 cut(s) 243, 304, 387, 600
BsrI ACTGG 4 cut(s) 127, 432, 977, 1054
BssECI CCNNGG 1 cut(s) 351
BssMI GATC 3 cut(s) 27, 231, 388
BssNAI GTATAC 1 cut(s) 983
Bst1107I GTATAC 1 cut(s) 983
Bst2UI CCWGG 1 cut(s) 612
Bst4CI ACNGT 4 cut(s) 86, 152, 868, 963
BstC8I GCNNGC 1 cut(s) 877
BstDEI CTNAG 2 cut(s) 249, 755
BstDSI CCRYGG 1 cut(s) 351
BstF5I GGATG 2 cut(s) 60, 982
BstH2I RGCGCY 2 cut(s) 74, 359
BstHHI GCGC 2 cut(s) 73, 358
BstKTI GATC 3 cut(s) 30, 234, 391
BstMAI GTCTC 1 cut(s) 179
BstMBI GATC 3 cut(s) 27, 231, 388
BstMWI GCNNNNNNNGC 3 cut(s) 362, 374, 551
BstNI CCWGG 1 cut(s) 612
BstNSI RCATGY 2 cut(s) 272, 879
BstSCI CCNGG 1 cut(s) 610
BstSFI CTRYAG 1 cut(s) 82
BstV1I GCAGC 3 cut(s) 389, 435, 1010
BstZ17I GTATAC 1 cut(s) 983
BtgI CCRYGG 1 cut(s) 351
BtgZI GCGATG 2 cut(s) 182, 353
BtsCI GGATG 2 cut(s) 60, 982
BtsI GCAGTG 2 cut(s) 333, 822
BtsIMutI CAGTG 3 cut(s) 91, 333, 822
Cac8I GCNNGC 1 cut(s) 877
CfoI GCGC 2 cut(s) 73, 358
Cfr13I GGNCC 1 cut(s) 893
Csp6I GTAC 2 cut(s) 608, 679
CspCI CAANNNNNGTGG 2 cut(s) 340, 375
CviAII CATG 2 cut(s) 269, 876
CviQI GTAC 2 cut(s) 608, 679
DdeI CTNAG 2 cut(s) 249, 755
DpnI GATC 3 cut(s) 29, 233, 390
DpnII GATC 3 cut(s) 27, 231, 388
DraI TTTAAA 1 cut(s) 439
DraIII CACNNNGTG 1 cut(s) 923
DriI GACNNNNNGTC 1 cut(s) 696
Eam1105I GACNNNNNGTC 1 cut(s) 696
Eco31I GGTCTC 1 cut(s) 179
Eco47I GGWCC 1 cut(s) 893
Eco47III AGCGCT 1 cut(s) 72
Eco57I CTGAAG 3 cut(s) 79, 648, 1014
EcoRII CCWGG 1 cut(s) 610
EcoT22I ATGCAT 1 cut(s) 547
FaeI CATG 2 cut(s) 272, 879
FaqI GGGAC 1 cut(s) 273
FatI CATG 2 cut(s) 268, 875
FauNDI CATATG 1 cut(s) 178
FbaI TGATCA 2 cut(s) 27, 231
FblI GTMKAC 1 cut(s) 982
Fnu4HI GCNGC 4 cut(s) 366, 378, 449, 999
FokI GGATG 2 cut(s) 47, 989
Fsp4HI GCNGC 4 cut(s) 366, 378, 449, 999
FspBI CTAG 3 cut(s) 374, 416, 503
GlaI GCGC 2 cut(s) 72, 357
GluI GCNGC 4 cut(s) 366, 378, 449, 999
GsuI CTGGAG 3 cut(s) 478, 822, 953
HaeII RGCGCY 2 cut(s) 74, 359
HapII CCGG 1 cut(s) 207
HhaI GCGC 2 cut(s) 73, 358
Hin1II CATG 2 cut(s) 272, 879
Hin6I GCGC 2 cut(s) 71, 356
HinP1I GCGC 2 cut(s) 71, 356
HincII GTYRAC 1 cut(s) 787
HindII GTYRAC 1 cut(s) 787
HindIII AAGCTT 1 cut(s) 485
HinfI GANTC 2 cut(s) 107, 683
HpaII CCGG 1 cut(s) 207
HphI GGTGA 3 cut(s) 9, 116, 767
Hpy166II GTNNAC 2 cut(s) 787, 983
Hpy188I TCNGA 5 cut(s) 220, 660, 667, 702, 851
Hpy188III TCNNGA 4 cut(s) 284, 292, 839, 932
Hpy8I GTNNAC 2 cut(s) 787, 983
HpyAV CCTTC 2 cut(s) 462, 644
HpyCH4III ACNGT 4 cut(s) 86, 152, 868, 963
HpyCH4V TGCA 8 cut(s) 89, 302, 462, 480, 545, 800, 815, 959
HpyF10VI GCNNNNNNNGC 3 cut(s) 362, 374, 551
HpyF3I CTNAG 2 cut(s) 249, 755
Hsp92II CATG 2 cut(s) 272, 879
HspAI GCGC 2 cut(s) 71, 356
KpnI GGTACC 1 cut(s) 611
Ksp22I TGATCA 2 cut(s) 27, 231
Kzo9I GATC 3 cut(s) 27, 231, 388
LmnI GCTCC 3 cut(s) 146, 221, 347
Lsp1109I GCAGC 3 cut(s) 389, 435, 1010
LweI GCATC 3 cut(s) 203, 352, 532
MaeI CTAG 3 cut(s) 374, 416, 503
MalI GATC 3 cut(s) 29, 233, 390
MboI GATC 3 cut(s) 27, 231, 388
MboII GAAGA 6 cut(s) 3, 6, 85, 157, 160, 462
MhlI GDGCHC 1 cut(s) 310
MluCI AATT 8 cut(s) 331, 475, 579, 653, 703, 845, 901, 1037
MlyI GAGTC 1 cut(s) 677
MnlI CCTC 9 cut(s) 47, 50, 183, 518, 697, 878, 884, 928, 1025
Mph1103I ATGCAT 1 cut(s) 547
MseI TTAA 4 cut(s) 438, 578, 710, 1040
MslI CAYNNNNRTG 1 cut(s) 1006
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 1 cut(s) 612
Mva1269I GAATGC 1 cut(s) 1048
MvaI CCWGG 1 cut(s) 612
MwoI GCNNNNNNNGC 3 cut(s) 362, 374, 551
NdeI CATATG 1 cut(s) 178
NdeII GATC 3 cut(s) 27, 231, 388
NlaIII CATG 2 cut(s) 272, 879
NlaIV GGNNCC 2 cut(s) 349, 609
NsiI ATGCAT 1 cut(s) 547
NspI RCATGY 2 cut(s) 272, 879
PaeI GCATGC 1 cut(s) 879
PciI ACATGT 1 cut(s) 268
PcsI WCGNNNNNNNCGW 1 cut(s) 537
PctI GAATGC 1 cut(s) 1048
PfeI GAWTC 1 cut(s) 107
PkrI GCNGC 4 cut(s) 367, 379, 450, 1000
PleI GAGTC 1 cut(s) 677
PpsI GAGTC 1 cut(s) 677
PscI ACATGT 1 cut(s) 268
PshBI ATTAAT 1 cut(s) 1040
Psp6I CCWGG 1 cut(s) 610
PspGI CCWGG 1 cut(s) 610
PspN4I GGNNCC 2 cut(s) 349, 609
PspPI GGNCC 1 cut(s) 893
RsaI GTAC 2 cut(s) 609, 680
RsaNI GTAC 2 cut(s) 608, 679
RseI CAYNNNNRTG 1 cut(s) 1006
SaqAI TTAA 4 cut(s) 438, 578, 710, 1040
SatI GCNGC 4 cut(s) 366, 378, 449, 999
Sau3AI GATC 3 cut(s) 27, 231, 388
Sau96I GGNCC 1 cut(s) 893
SchI GAGTC 1 cut(s) 677
ScrFI CCNGG 1 cut(s) 612
SduI GDGCHC 1 cut(s) 310
SfaNI GCATC 3 cut(s) 203, 352, 532
SfcI CTRYAG 1 cut(s) 82
SinI GGWCC 1 cut(s) 893
SmiMI CAYNNNNRTG 1 cut(s) 1006
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
SphI GCATGC 1 cut(s) 879
Sse9I AATT 8 cut(s) 331, 475, 579, 653, 703, 845, 901, 1037
SsiI CCGC 1 cut(s) 365
SspMI CTAG 3 cut(s) 374, 416, 503
StyD4I CCNGG 1 cut(s) 610
TaaI ACNGT 4 cut(s) 86, 152, 868, 963
TaqI TCGA 3 cut(s) 110, 689, 870
TasI AATT 8 cut(s) 331, 475, 579, 653, 703, 845, 901, 1037
TauI GCSGC 1 cut(s) 368
TfiI GAWTC 1 cut(s) 107
Tru1I TTAA 4 cut(s) 438, 578, 710, 1040
Tru9I TTAA 4 cut(s) 438, 578, 710, 1040
TscAI CASTG 3 cut(s) 91, 333, 822
TseI GCWGC 3 cut(s) 377, 448, 998
TspDTI ATGAA 3 cut(s) 185, 232, 684
TspRI CASTG 3 cut(s) 91, 333, 822
VpaK11BI GGWCC 1 cut(s) 893
VspI ATTAAT 1 cut(s) 1040
XapI RAATTY 2 cut(s) 331, 653
XceI RCATGY 2 cut(s) 272, 879
XmiI GTMKAC 1 cut(s) 982
XspI CTAG 3 cut(s) 374, 416, 503
Zsp2I ATGCAT 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.