Rorug04G0449600

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001486
Physical Location & Seq
Forward (+)
60457948 .. 60469137
11190 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0449600.1

Sequence Viewer

Length: 7863 bp
ATGGAAGGTTCACACAAGAAAAAGGGTAAAAGACCCAACCTTTTGGCCGATACTGTGTTTTCTTGGTCCCTTGATGACATTTTCAACGAACGTCTCTTCAAGAACAAGGTGGAAAAGATTCCTGAATCATTTCACTCTGTTGAGCATTACTTCGGGTGTTACATGTATCCTTTATTGGAAGAAACACGAGCGCAAGTCCATTCGAGTATGGAAACTATTTACAGAGCACCATTTGCTAAAGTAGTTGCTTTTGAAAAAGCCAAGCCATATGGAACAAAGCTATATAATATTAAGGTTGATTACTGGCGAAACAGGTTCAATGACCGTGGCAAGGAGCCATACAAAACTTTGCCTGGTGATCTTTTTGTTTTAGCAAATGCTAAACCTGAAACTGTTTCAGATTTACAAAGGGTAGGGAGGTCATGGGCTTTTGCATCGGTCACTAAAGTCTCGGAAAATGAGAACAAGGATGACACTACTTCTCTTTATTTTAAAGTCAAGGCTTCCAAAGAGCTTGACGTCGTAAAGAGCACAACACCACTGTTTATGGTTTTTCTAGTCAACTTAATCCCAAATGGAAGAATATGGAAAGCTTTGCACATGTCCAAAAACCTGAAGATTATCAAGGAGGTTCTGTGCACTGATTCTGTGGCTCAGAAAAATCTCTGCTCTGAAAAGAACAATGACATTGTGAATAAGAGATTAGTTCAGAGTTTATCATCTGGTTTGAATGAATCCCAGACTGGGACCGTTCTGGCCTGTCTTGAAATGCTGCATTCTCGTGAAAAGTCTGCTGTGGAACTAATTTGGGGTCCTCCTGGTACTGGAAAAACTAAAACTATCGTGACTCTACTTTTAACCCTGCTACAGATGAATTGTAGGACTCTTGTCTGTGCCCCAACAAATGTTGCAATAACTGAAGTTGCTTCTCGCGTTGTGAAGATGGTGACTGAAGTAGAGTCCAGTGCTTTGTTTTGTTCTTTGGGAGAAGTTCTTCTATTTGGGAATAAGGAGCGACTCAAAGTTGGCTCAGACATTGAAGAGATATATTTAGATTACCGTCTCAAAAGGCTTGTTGAGTGCTTAGGGCCACGGACTGGTTGGAGGCATTGTTTTGCTTCCATGATAGATTGTCTTGAAGATGGTGTTTCTCACTACCATACTTTTTTGGAAAACGAGTTTACTATTGAGAAGGAACCAAAGGCTATAGGTCAAATGAAAGCGGAAGAAAGCAGAATTGTCGCTAAAGCTGGCAAGGACACATGCAAAACATTTCTGGAATTTATTAGAGACAGATTTGTTACTACTGCATCACCTCTAAGATATTGCCTTTCTATCTTCTGTACCCACATGCCGAAAAATTACATTTCTGTAGATAATTTCCAAAACATGGTTTTGCTTATCAAATTGGTTGACTCCTTTGAATCATTGTTGTTTCAAGTTAATGTTGCTTCTGAAGCACTTGAAGACATTTTCTCACGTTCTGAAGTTGAAGACATATCAGACACATGTGCTGATAACTCGTTTCTTCTATTCACAAATAGAAGAGAATGCCTTAAAGTCTTACACAATCTATTTGACTCTCTGAGTGAACTTCACCTTCCGGATTTTATGAATCAAGAGTCTCTAATGGCATTTTGTTTTCAAAGTGCTTCCTTGATATTTTGCACTGCATCCAGCTCATATAAGTTGCATAAATTGGCAATGGAGCCACTAAGCATTGTGGTTATTGACGAAGCTGCGCAATTGAAAGAGTGTGAATCAACAATCCCCCTTCAACTACCAGGCGTGAAGCATGCTGTGCTTGTTGGTGATGAATGTCAGTTACCAGCTATAGTGAACAGCATTGTTTCTGATGAAGCTGGTTTTGCAAGAAGCTTGTTTGAGAGGTTAAGCTCAGTTGGTCACTCAAAACACCTCCTAAATATGCAGTACAGGATGCATCCATCAATAAGTTTCTTCCCAAATTCTAGTTTCTACAATAACATGATCCTGGATGCTCCAAATCTTAAAAGGAGAAGCCAGGAGAAAAACTATCTTAAAGGGTTAATGTTTGGTCCTTATTCTTTTATCAATGTCATTGGTGGACGAGAAGAGAAGGATGAGGATGGACGTAGTCGAAAGAATATGGTTGAGGTTGCTATTGTTTCACAAATACTGCGGAAACTGTATAAAGAATGGGTTGATTCAAAACAGAATCTCAGTATTGGTATAGTATCTCCATATGCTGCTCAAGTAGTTGCAATCGAGGACAAACTTGGACAGAAGTATAATAATCTTGATGGATTTATAGTGAAGGTGAAGACAGTTGATGGGTTCCAGGGTGGGGAAGAGGACATCATTATATTTTCCACTGTACGATCCAATTGTCAACAGTCACTTGAGTTTATTTCAAAACCGCAAAGAATTAATGTTGCTCTTACAAGGGCTAGGCACTGTCTGTGGATTTTGGGGAATGAAAGAACTCTATGTGATAGTGAGTCTGTTTGGGAGGCCTTGGTCCTTGATGCCAAAAATCGCCATTGCTTTTTCAATGCTGATGAAGACAAGGATTTAGCCAAGGCCATATTAGAGGTGAAGAAACAGTTCGACCAACTAGATGATTTGCTTAATGCTGACAGTGTACTTTTCAGAAGTGCTAGATGGAAGGTACTTTTTAGTGATAACTTTTTGAAGTCATTCAAGAAACTGATGTCAGTCCGTTTGAAGAGGTCAGTGCTAAATGTTCTACTAAAGCTTGCCAGTGGCTGGAGACCGAAGATGCAAAATTCAGATATAATCTGTGGCAACTCTTCACTGATCTTGAAGAAATTCAAGGTTGAAGATCTATATATTGTTTGCACAACAGACATAGCAAAGGATTTGAGATACATTCAAGTATTGAAGATTTGGGACATATTGCCTATAGAGGATGTCCCAACGTTGGTAAATCGCCTGGACAATATATTAAACAAATATACAGATGATTATATTAATCTCTGCAAGGAGAAATGTCTTGAGGGTGATTTGGAAGTTCCAAAGAGCTGGCCAAGTTCTTTGGAATTTATTCGGTTTAAGGATCTTAGCTTCACTGAAATCCAGAATGATTTAGTTGGTGACACTTCTCATGGTAGAAGTTATGCTGAGAATTCACGTGTCAGCGAAAGTTTGTTACTGATGAAATTTTACTCACTATCATCTGGGGTGGTAAACCACTTGCTGTCTGATCATGAGGGTAGAGAACTGGATCTTCCGTTTGAAGTTACAGACCAAGAATTGGAGATTATCCTTTATCAGAGAAGTTCCTTTATAGTTGGACGGTCAGGAACTGGGAAAACCACAGTTTTAACCATGAAATTATTTCAGAAAGAGCAGTGGCACCAGTTGGCAGTGCGAGGATGCCATATTAGTGAAAGCAACAAGGTTGAACAGAGTTCTGCAGCCACTGAGGGGAAAGTTCTACACCAGCTTTTCGTGACAGTGAGTCCGAAGCTATGTTATGCTATCAAGCAACATGTTTTACACTTGAAAAGATTTGCATCTGGTAGAAGTCATTCAACTGAAACCGGTTTGATTGATATGATTGATTTTGATGAGGATGAAGCTCAATTTAACGATATCCAAGATTCATTTCATGAAGTTCCTGCCCATTCTTACCCTCTTTTCATCACATTGCATAAGTTCTTGATTATGCTTGATGGAACATTGAGAAACTCATACTTTGAAAGATTCCTTGATGCAACTGAGGTTACTCATGGTCAACCACGAAGTTCAAGATCAATTGCCTTGCAGACTTTTATAAGGACAAAGGAAGTCAGTTATGAGAAGTTTAGTTCATTATACTGGCCTCATTTTAATACCGAGTTAACAAAGAAGCTTGATGCTTCAAGAGTCTTTACAGAGATTATTTCTCATATAAAAGGTGGCCTGGGAGCTCTAGAAGCGTGTGATGGTAAATTGAGTCGAGAGGATTATGTTCAACTGTCAGAGAGCCGAGCTTCCAATCTAACCAGCCAAGAGAGAGAAGTTATATATGACATTTTTCAGATGTATGAAAAAATGAAGTTGGAATATGGTGAATTTGATCTTGCTGATTTTGTTACTGATCTTCACCGTCGACTCAGGCATGAAAAGTATGAGGGGGATCATATGGACTTTGTTTATATTGATGAGGTTCAGGATCTCACAATGAGTCAAATTGCCTTGTTTAAACACATGTGCCACAATGTTGAAGAGGGTTTTGTTTTTTCGGGTGATACTGCTCAAACAATTGCAAGGGGTATTGATTTCAGATTTCAAGAATTACGACATCTATTCTACAAGAAATTTGTGTTGGAGTCAAGAAGCAATAAGCACGATGAAAGTAAGGAAAAGGGCCAAATCTCAGAAATTTTTCACTTGACTCAAAACTTTCGTACCCATGCTGGAATCCTCAAGCTATCACAAAGCATTGTTGAACTTCTTTATCATTTTTTCCCCCTTTCCATTGATGTTTTAAAACCTGAAAGCAGTTTGATATATGGGGAAGCTCCAGTATTGCTTGAATCTGGAGAAAATGAAAATGCAATCGTAAAGATATTTGGAAATACTGGAAATATAGTTGGATTTGGTGCTGAGCAGGTAATTTTGGTGCGAGATGATAGTGCTCGAAATGAGATATCAAAACTTGTAGGGAAGCATGCTCTTGTGCTAACCATTGTCGAGTGCAAGGGCCTTGAGTTTCAGGATGTTCTCTTATACAACTTTTTCGGATCATCACCTTCGAGAAATCAATGGAGGGTAATATATGATTACATGAATGAACTAGATTTACTCGACCACACATTACCCAGGGGCTTTCCAAGTTTTAATGTAGCCAAACACAACATATTGTGCTCTGAACTGAAACAACTATATGTGGCTGTTACTCGCACGAGACAGAGATTGTGGATATGTGAGAACTTTGAAGATCTCTCCAAACCAATGTTTGACTACTGGAAGAAGAAGTGTCTTGTGCAAGTGAAGCAACTGGATGATTCCCTTGCACATGCAATGCAAGTTGCAAGCAGTCCAGAGGAGTGGAAGTCACGTGGCATGAAGCTATATCATGAACATAACTACGAAATGGCCACAATGTGCTTTGAAAGAGCCGGTGATGCTTACTGGGAAAGAAGGTCTAAAGCTGCTGGCCTTAAAGCACTCGCTGACCATATGCGAACGTCAAATCCTGAAGAGGCTAAATCTATTCTCAGGGAAGCTGCTGAAATATTTGATGCTATAGGCAAGGCAGATTCTGCGGCGAGATGTTTTTCTGATTTGGGGGAGTATGAAAGAGCTGCTAGGATATACTTGAACAAATGTGGCGAGTCTGAACTGGAGAGAGCCGCGGAATGTTTTTCTCTAGCAGGATGCTATGAGGAGGCTGCAGATGTGTATGCCAAGGGAAATTTTTTCTCAGAGTGTCTCACTGTTTGTGGCAAGGGAAGACTATTTGAAATGGGTTTGCAATACATCAATAATTGGAAACAGCATGCAGAAGAGGACAGTGCTATGACTACAAGGGGAAATGGAATAAATAGAATGGAGCATGAGTTTTTAGAGAGCTGTGCAGTTCACTATTATAATTTGAATGATAACAGATCCATGATGAAATTTGTTAAAGCTTTTCATTCTATAATTTTGATGCGAAACTTTTTGAAGAGGTTGGATAACCTTGATGAGCTTTTGTTGTTGGAGGAAGAATTTGGAAATTATCTGGAAGCTGCAAACATCGCACAGCTTAAAGGTGATATTCTACTTGAATCTGATTTCCTTGGAAAGGCAGGTAAGTTCAAAGAGGCCTCACTGCACATACTATTCTACGTACTTGCCGAGTCTCTTTGGTCATCTGGCAGCAAGGGCTGGCCTCTGAAGAAGTTTCCACGACAGGAGGCTCTTTTGGCACAAGCCAAGTCACTTGCCAAGAATGAGACAGACAACTTTTATGAATTGGTTTCCACTGAGGTTGATATTTTATTAAATGGGATGGATAGGTTGGCTGTTTTGAAAAATCAAATCAATTCTTCTGAGAGACATAAGAGTATTAGAGGGGAAGTGTTATCAGCTAGAAAAATTTTGGATGCCCATCTTTCTCGAAGAACTGATAAGTATATATGGGAAACAGAATTGATTGGTGATCTTGTACAGCATTCAGAAGAGAAAATATCTAAAAATCAAGTTTCCATCGATTCACTCATCTACTTCTGGAATTCTTGGAAGGAGAAGATTCTTCACATAATTGAATCTGTAGGTTGTTTGGAAACACAAGACTACAAAGGTTATGTAGAGTTTGGTTTTACTTTCTTAGGAGTCTGGAGGCTGTATCATAATCTGAGTCCAGTGTATGTTTTACTCATCTCTGATGCTGATTGGGTTAGAGGTCTGGACAAAAGACATTTCCAAAGTTATGAGAAGTTGGTCTCCATTGATGTTCACCAACTCATTCCAGCTGCTCAAAATTTTTGGTGTTCAGAACTGGTCTCTGTTGGCATTAAGGTTTTGGAAAAGCTTGAAGCACTTTTCAAGTTCCCAGTCAAGAGTCCTGATTCAATTTTCTTTCAAAGCAGGGCTCTTACTCTGATCTATGATGTTGGAAAATATCTTTTGGATTCCAAGTGTCTGAAGCAAAGGAATCAAGATACCATGACACTACAGAAGTTTGTAACATTTTCGACTGAAAAATATATTACTTGCATATTTCCTTTAGATTGGAGGAATTCAGCAAGAGAGAATATGATTTCTCTTAGAAGAACTAATGCTTCCCAATCTATGCTGAAACAAGTAATAGTTGAATGTATCAGCGGGAAGAACAAGATGTCTTATGGGCAAATTGGAAAGGTCATCATGATCATTCTTGGGTCTGGCAAGCCAAGTAGTGAACTTCACAGCATGATTGTGCAAAAGCTGACCTGCAACCCGCCATGGATGTCATTCGTTGAGACTTTGTGTGAGAAGGATATGGGACCTTGGAGTCGTAGTGGAGAACCTAAACAGACGTCCGTTTTGTGGCGATTCTATGAAGCATTAGTCGAAACTTATAGTGCAAACTGGAGGGCTCTTCATGACTACATATCACCTGGTTGCTTCTTGTATCTTGTAGAGCGCCTTTTGACTTTGGCCTCTTGCTTTCATGGTTTTGTTATCTCCACGAGTTCAAGTTTTATAGAATGGCTGATATACAAGGATGGAGATACCATCATAAGCTCACCATCCCTGGGAGAGATTGCTGATTTTGTGACTGATGTTGTTCGAGATTGTATTTATAAAAGGGGGAATATGACTGATTGGATTAGAAAAACTGATGCAGATGGGAAGAGTTCTTATCCACTACTCATGTTGAGATTGGTTATTCTACTATGCTTGCTTCTTGTGAACTTTGGGAAGTCTTTAGATATGCTCTTTGATATTCTGGGGAAGAACTATGTCATTGGAGTACTGCCCAAGGAATTTGTTGAGGCCCTTAAGAAGGGGAGGAAAGACAGATCTGCTAGTGCCTGTGTGCTAGCTATAGCTACTGCTCTTAAGAAGATTGGTAATCCTTTAGTAATTGCAAGTTTCGGGTTCAATTGTTCAAGGTTCTTATGCACAGATGCCATTTATGTTGACATGGTATTAAACCCGAGCAAGGAGGACGTACTTGGGAAATTCTTCCCTAAACCACCTGTTGTCCCAGCTTCACAAAGCCGAATGAGACCTGTTGAAGGGGAAGGTAATAATCGTTATACTGTTCTGTCGGATGCTGAAGACACTCACAGTGAAGATGAGGAGTCGAAGCCGAAGTGCCCAGAACCTGATGCTGCTTCTGCAACCCATGGGAAGAAAAAGGGCAATCCCAAGAAACACAAGGGGAAAAAGGGTCGAAAGTAG

Protein Analysis

2620

Amino Acids

299.1

Weight (kDa)

6.52

Isoelectric Point (pI)

42.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 77 - 205 2.5e-64 Domain of unknown function (DUF6469)
AAA_11 PF13086 243 - 615 8.6e-34 AAA domain
AAA_19 PF13245 263 - 612 4.1e-07 AAA domain
AAA_12 PF13087 624 - 820 3.5e-57 AAA domain
UvrD-helicase PF00580 1323 - 1417 5.2e-07 UvrD/REP helicase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 3779, 5580, 7260
AasI GACNNNNNNGTC 1 cut(s) 6966
AatII GACGTC 2 cut(s) 522, 6995
Acc16I TGCGCA 1 cut(s) 1743
Acc36I ACCTGC 3 cut(s) 4588, 5771, 6914
AccB1I GGYRCC 1 cut(s) 3357
AccB7I CCANNNNNTGG 4 cut(s) 1097, 1390, 2748, 3256
AccI GTMKAC 1 cut(s) 4096
AccII CGCG 2 cut(s) 933, 5345
AccIII TCCGGA 1 cut(s) 1603
AciI CCGC 8 cut(s) 1223, 2161, 2399, 5255, 5343, 5345, 6798, 6914
AclI AACGTT 1 cut(s) 2921
AclWI GGATC 8 cut(s) 1984, 2355, 3066, 3234, 4131, 4167, 4738, 5592
AcoI YGGCCR 3 cut(s) 45, 3026, 5085
AcuI CTGAAG 9 cut(s) 635, 939, 972, 1476, 1506, 5208, 5888, 6638, 7758
AcvI CACGTG 2 cut(s) 3134, 5048
AcyI GRCGYC 2 cut(s) 519, 6992
AdeI CACNNNGTG 1 cut(s) 5094
AflII CTTAAG 2 cut(s) 7457, 7517
AflIII ACRYGT 6 cut(s) 162, 600, 1508, 3133, 3493, 4194
AgeI ACCGGT 1 cut(s) 3545
AhdI GACNNNNNGTC 1 cut(s) 3462
AjuI GAANNNNNNNTTGG 4 cut(s) 3594, 3626, 4295, 4327
AloI GAACNNNNNNTCC 2 cut(s) 5167, 5199
Alw21I GWGCWC 6 cut(s) 229, 533, 641, 3916, 4627, 4856
Alw44I GTGCAC 1 cut(s) 637
AlwI GGATC 8 cut(s) 1984, 2355, 3066, 3234, 4131, 4167, 4738, 5592
AlwNI CAGNNNCTG 5 cut(s) 2748, 3308, 3425, 5252, 7787
Ama87I CYCGRG 1 cut(s) 7615
Aor13HI TCCGGA 1 cut(s) 1603
ApaLI GTGCAC 1 cut(s) 637
ArsI GACNNNNNNTTYG 2 cut(s) 6982, 7014
AseI ATTAAT 2 cut(s) 2409, 2973
AsiGI ACCGGT 1 cut(s) 3545
AspLEI GCGC 3 cut(s) 193, 1744, 7101
AsuNHI GCTAGC 1 cut(s) 7498
AvaI CYCGRG 1 cut(s) 7615
AvaII GGWCC 6 cut(s) 66, 747, 812, 2057, 2500, 6959
BaeGI GKGCMC 3 cut(s) 641, 898, 7781
BaeI ACNNNNGTAYC 2 cut(s) 4378, 4411
BalI TGGCCA 2 cut(s) 3028, 5087
BanI GGYRCC 1 cut(s) 3357
BanII GRGCYC 3 cut(s) 3916, 6568, 7054
BauI CACGAG 4 cut(s) 186, 780, 4891, 7144
BbrPI CACGTG 2 cut(s) 3134, 5048
BbsI GAAGAC 6 cut(s) 1473, 1500, 2309, 2550, 5449, 7746
Bbv12I GWGCWC 6 cut(s) 229, 533, 641, 3916, 4627, 4856
BcgI CGANNNNNNTGC 2 cut(s) 1222, 1256
BciVI GTATCC 1 cut(s) 177
BclI TGATCA 2 cut(s) 3205, 6843
BfoI RGCGCY 1 cut(s) 7102
BfrI CTTAAG 2 cut(s) 7457, 7517
BfuAI ACCTGC 3 cut(s) 4588, 5771, 6914
BfuI GTATCC 1 cut(s) 177
BglII AGATCT 3 cut(s) 2824, 4927, 7478
BlpI GCTNAGC 1 cut(s) 4593
BmcAI AGTACT 1 cut(s) 7431
Bme18I GGWCC 6 cut(s) 66, 747, 812, 2057, 2500, 6959
BmeRI GACNNNNNGTC 1 cut(s) 3462
BmeT110I CYCGRG 1 cut(s) 7615
BmiI GGNNCC 9 cut(s) 68, 336, 748, 813, 1197, 1710, 2318, 3359, 6960
BmrI ACTGGG 4 cut(s) 753, 3318, 5131, 6521
BmtI GCTAGC 1 cut(s) 7502
BmuI ACTGGG 4 cut(s) 753, 3318, 5131, 6521
BoxI GACNNNNGTC 1 cut(s) 887
BpiI GAAGAC 6 cut(s) 1473, 1500, 2309, 2550, 5449, 7746
BpmI CTGGAG 6 cut(s) 2770, 4494, 4548, 5354, 6331, 7066
Bpu10I CCTNAGC 1 cut(s) 1084
Bpu1102I GCTNAGC 1 cut(s) 4593
BpuEI CTTGAG 5 cut(s) 2217, 2402, 3017, 4397, 4715
Bsa29I ATCGAT 1 cut(s) 6183
BsaAI YACGTR 3 cut(s) 3134, 5048, 5821
BsaBI GATNNNNATC 2 cut(s) 3517, 6081
BsaHI GRCGYC 2 cut(s) 519, 6992
BsaI GGTCTC 4 cut(s) 2746, 6421, 6481, 7681
BsaWI WCCGGW 2 cut(s) 1603, 3545
Bse118I RCCGGY 2 cut(s) 3545, 5108
Bse3DI GCAATG 4 cut(s) 1710, 2521, 3650, 5016
Bse8I GATNNNNATC 2 cut(s) 3517, 6081
BseAI TCCGGA 1 cut(s) 1603
BseCI ATCGAT 1 cut(s) 6183
BseJI GATNNNNATC 2 cut(s) 3517, 6081
BseMI GCAATG 4 cut(s) 1710, 2521, 3650, 5016
BseRI GAGGAG 3 cut(s) 5048, 5390, 7775
BseSI GKGCMC 3 cut(s) 641, 898, 7781
BseYI CCCAGC 1 cut(s) 7666
BsgI GTGCAG 2 cut(s) 5586, 5789
Bsh1236I CGCG 2 cut(s) 933, 5345
BshNI GGYRCC 1 cut(s) 3357
BshTI ACCGGT 1 cut(s) 3545
BshVI ATCGAT 1 cut(s) 6183
BsiHKAI GWGCWC 6 cut(s) 229, 533, 641, 3916, 4627, 4856
BsiHKCI CYCGRG 1 cut(s) 7615
BsiSI CCGG 3 cut(s) 1604, 3546, 5109
BslFI GGGAC 6 cut(s) 52, 760, 2900, 2906, 6972, 7649
BsmBI CGTCTC 2 cut(s) 98, 1067
BsmFI GGGAC 6 cut(s) 52, 760, 2900, 2906, 6972, 7649
BsmI GAATGC 3 cut(s) 775, 1556, 6145
Bso31I GGTCTC 4 cut(s) 2746, 6421, 6481, 7681
BsoBI CYCGRG 1 cut(s) 7615
Bsp13I TCCGGA 1 cut(s) 1603
Bsp1407I TGTACA 1 cut(s) 6139
Bsp1720I GCTNAGC 1 cut(s) 4593
Bsp19I CCATGG 2 cut(s) 6917, 7807
BspACI CCGC 8 cut(s) 1223, 2161, 2399, 5255, 5343, 5345, 6798, 6914
BspDI ATCGAT 1 cut(s) 6183
BspEI TCCGGA 1 cut(s) 1603
BspFNI CGCG 2 cut(s) 933, 5345
BspHI TCATGA 5 cut(s) 3208, 3613, 5065, 6840, 7057
BspLI GGNNCC 9 cut(s) 68, 336, 748, 813, 1197, 1710, 2318, 3359, 6960
BspMAI CTGCAG 2 cut(s) 3421, 5386
BspMI ACCTGC 3 cut(s) 4588, 5771, 6914
BspOI GCTAGC 1 cut(s) 7502
BspPI GGATC 8 cut(s) 1984, 2355, 3066, 3234, 4131, 4167, 4738, 5592
BspQI GCTCTTC 1 cut(s) 7059
BspT107I GGYRCC 1 cut(s) 3357
BspTI CTTAAG 2 cut(s) 7457, 7517
BspTNI GGTCTC 4 cut(s) 2746, 6421, 6481, 7681
BsrDI GCAATG 4 cut(s) 1710, 2521, 3650, 5016
BsrFI RCCGGY 2 cut(s) 3545, 5108
BsrGI TGTACA 1 cut(s) 6139
BssAI RCCGGY 2 cut(s) 3545, 5108
BssNI GRCGYC 2 cut(s) 519, 6992
BssSI CACGAG 4 cut(s) 186, 780, 4891, 7144
BssT1I CCWWGG 8 cut(s) 2496, 2559, 5397, 5770, 6917, 6962, 7437, 7807
Bst2BI CACGAG 4 cut(s) 186, 780, 4891, 7144
BstACI GRCGYC 2 cut(s) 519, 6992
BstAFI CTTAAG 2 cut(s) 7457, 7517
BstAPI GCANNNNNTGC 3 cut(s) 233, 1801, 5252
BstAUI TGTACA 1 cut(s) 6139
BstBAI YACGTR 3 cut(s) 3134, 5048, 5821
BstDSI CCRYGG 5 cut(s) 325, 1091, 5343, 6917, 7807
BstENI CCTNNNNNAGG 3 cut(s) 5774, 7460, 7695
BstFNI CGCG 2 cut(s) 933, 5345
BstH2I RGCGCY 1 cut(s) 7102
BstHHI GCGC 3 cut(s) 193, 1744, 7101
BstPAI GACNNNNGTC 1 cut(s) 887
BstSLI GKGCMC 3 cut(s) 641, 898, 7781
BstSNI TACGTA 1 cut(s) 5821
BstUI CGCG 2 cut(s) 933, 5345
BstV2I GAAGAC 6 cut(s) 1473, 1500, 2309, 2550, 5449, 7746
BstX2I RGATCY 7 cut(s) 2824, 3058, 3226, 4159, 4927, 5597, 7478
BstXI CCANNNNNNTGG 3 cut(s) 43, 3024, 5037
BstYI RGATCY 7 cut(s) 2824, 3058, 3226, 4159, 4927, 5597, 7478
Bsu15I ATCGAT 1 cut(s) 6183
BsuI GTATCC 1 cut(s) 177
BsuTUI ATCGAT 1 cut(s) 6183
BtgI CCRYGG 5 cut(s) 325, 1091, 5343, 6917, 7807
BtgZI GCGATG 1 cut(s) 5713
BtsI GCAGTG 4 cut(s) 1668, 3359, 3375, 5801
BveI ACCTGC 3 cut(s) 4588, 5771, 6914
CaiI CAGNNNCTG 5 cut(s) 2748, 3308, 3425, 5252, 7787
CciI TCATGA 5 cut(s) 3208, 3613, 5065, 6840, 7057
CfoI GCGC 3 cut(s) 193, 1744, 7101
Cfr10I RCCGGY 2 cut(s) 3545, 5108
Cfr42I CCGCGG 1 cut(s) 5346
ClaI ATCGAT 1 cut(s) 6183
CsiI ACCWGGT 1 cut(s) 7072
CspAI ACCGGT 1 cut(s) 3545
CspCI CAANNNNNGTGG 4 cut(s) 307, 342, 5944, 5979
DraI TTTAAA 3 cut(s) 493, 4189, 4476
DraIII CACNNNGTG 1 cut(s) 5094
DrdI GACNNNNNNGTC 1 cut(s) 6966
DriI GACNNNNNGTC 1 cut(s) 3462
DseDI GACNNNNNNGTC 1 cut(s) 6966
EaeI YGGCCR 3 cut(s) 45, 3026, 5085
Eam1105I GACNNNNNGTC 1 cut(s) 3462
Ecl136II GAGCTC 1 cut(s) 3914
Eco105I TACGTA 1 cut(s) 5821
Eco130I CCWWGG 8 cut(s) 2496, 2559, 5397, 5770, 6917, 6962, 7437, 7807
Eco147I AGGCCT 2 cut(s) 2495, 5798
Eco24I GRGCYC 3 cut(s) 3916, 6568, 7054
Eco31I GGTCTC 4 cut(s) 2746, 6421, 6481, 7681
Eco32I GATATC 2 cut(s) 3598, 4638
Eco47I GGWCC 6 cut(s) 66, 747, 812, 2057, 2500, 6959
Eco53kI GAGCTC 1 cut(s) 3914
Eco57I CTGAAG 9 cut(s) 635, 939, 972, 1476, 1506, 5208, 5888, 6638, 7758
Eco72I CACGTG 2 cut(s) 3134, 5048
Eco88I CYCGRG 1 cut(s) 7615
EcoICRI GAGCTC 1 cut(s) 3914
EcoNI CCTNNNNNAGG 3 cut(s) 5774, 7460, 7695
EcoO109I RGGNCCY 4 cut(s) 812, 4690, 6959, 7453
EcoRI GAATTC 3 cut(s) 3127, 6205, 6712
EcoRV GATATC 2 cut(s) 3598, 4638
EcoT14I CCWWGG 8 cut(s) 2496, 2559, 5397, 5770, 6917, 6962, 7437, 7807
EcoT22I ATGCAT 1 cut(s) 1944
EcoT38I GRGCYC 3 cut(s) 3916, 6568, 7054
ErhI CCWWGG 8 cut(s) 2496, 2559, 5397, 5770, 6917, 6962, 7437, 7807
Esp3I CGTCTC 2 cut(s) 98, 1067
FaqI GGGAC 6 cut(s) 52, 760, 2900, 2906, 6972, 7649
FauI CCCGC 2 cut(s) 6791, 6921
FauNDI CATATG 4 cut(s) 268, 2224, 4128, 5169
FbaI TGATCA 2 cut(s) 3205, 6843
FblI GTMKAC 1 cut(s) 4096
FriOI GRGCYC 3 cut(s) 3916, 6568, 7054
FspI TGCGCA 1 cut(s) 1743
GlaI GCGC 3 cut(s) 192, 1743, 7100
GsaI CCCAGC 1 cut(s) 7670
GsuI CTGGAG 6 cut(s) 2770, 4494, 4548, 5354, 6331, 7066
HaeII RGCGCY 1 cut(s) 7102
HapII CCGG 3 cut(s) 1604, 3546, 5109
HhaI GCGC 3 cut(s) 193, 1744, 7101
Hin1I GRCGYC 2 cut(s) 519, 6992
Hin6I GCGC 3 cut(s) 191, 1742, 7099
HinP1I GCGC 3 cut(s) 191, 1742, 7099
HincII GTYRAC 7 cut(s) 562, 1414, 2372, 3740, 3846, 4097, 7600
HindII GTYRAC 7 cut(s) 562, 1414, 2372, 3740, 3846, 4097, 7600
HindIII AAGCTT 6 cut(s) 591, 1876, 2735, 3854, 5619, 6503
HpaI GTTAAC 1 cut(s) 3846
HpaII CCGG 3 cut(s) 1604, 3546, 5109
Hpy99I CGWCG 2 cut(s) 524, 4098
Hsp92I GRCGYC 2 cut(s) 519, 6992
HspAI GCGC 3 cut(s) 191, 1742, 7099
Kpn2I TCCGGA 1 cut(s) 1603
Ksp22I TGATCA 2 cut(s) 3205, 6843
KspAI GTTAAC 1 cut(s) 3846
KspI CCGCGG 1 cut(s) 5346
LguI GCTCTTC 1 cut(s) 7059
LmnI GCTCC 7 cut(s) 334, 1012, 1708, 2005, 3911, 4513, 5542
MabI ACCWGGT 1 cut(s) 7072
MfeI CAATTG 5 cut(s) 1745, 2365, 3759, 4248, 7561
MflI RGATCY 7 cut(s) 2824, 3058, 3226, 4159, 4927, 5597, 7478
MlsI TGGCCA 2 cut(s) 3028, 5087
MluNI TGGCCA 2 cut(s) 3028, 5087
MmeI TCCRAC 9 cut(s) 1082, 3274, 4026, 4293, 4561, 5643, 5670, 6568, 7710
Mox20I TGGCCA 2 cut(s) 3028, 5087
Mph1103I ATGCAT 1 cut(s) 1944
MroI TCCGGA 1 cut(s) 1603
MscI TGGCCA 2 cut(s) 3028, 5087
MslI CAYNNNNRTG 3 cut(s) 780, 3387, 6890
Msp20I TGGCCA 2 cut(s) 3028, 5087
MspA1I CMGCKG 3 cut(s) 5345, 6446, 6798
MspCI CTTAAG 2 cut(s) 7457, 7517
MspI CCGG 3 cut(s) 1604, 3546, 5109
MssI GTTTAAAC 1 cut(s) 4189
MunI CAATTG 5 cut(s) 1745, 2365, 3759, 4248, 7561
Mva1269I GAATGC 3 cut(s) 775, 1556, 6145
MvnI CGCG 2 cut(s) 933, 5345
NcoI CCATGG 2 cut(s) 6917, 7807
NdeI CATATG 4 cut(s) 268, 2224, 4128, 5169
NheI GCTAGC 1 cut(s) 7498
NlaIV GGNNCC 9 cut(s) 68, 336, 748, 813, 1197, 1710, 2318, 3359, 6960
NmeAIII GCCGAG 2 cut(s) 3998, 5854
NsbI TGCGCA 1 cut(s) 1743
NsiI ATGCAT 1 cut(s) 1944
PaeI GCATGC 3 cut(s) 1799, 4661, 5492
PagI TCATGA 5 cut(s) 3208, 3613, 5065, 6840, 7057
PasI CCCWGGG 2 cut(s) 4809, 7210
PceI AGGCCT 2 cut(s) 2495, 5798
PciI ACATGT 5 cut(s) 162, 600, 1508, 3493, 4194
PciSI GCTCTTC 1 cut(s) 7059
PcsI WCGNNNNNNNCGW 1 cut(s) 5826
PctI GAATGC 3 cut(s) 775, 1556, 6145
PflFI GACNNNGTC 1 cut(s) 2115
PflMI CCANNNNNTGG 4 cut(s) 1097, 1390, 2748, 3256
PfoI TCCNGGA 1 cut(s) 1992
PinAI ACCGGT 1 cut(s) 3545
PmaCI CACGTG 2 cut(s) 3134, 5048
PmeI GTTTAAAC 1 cut(s) 4189
PmlI CACGTG 2 cut(s) 3134, 5048
Ppu21I YACGTR 3 cut(s) 3134, 5048, 5821
PpuMI RGGWCCY 2 cut(s) 812, 6959
PscI ACATGT 5 cut(s) 162, 600, 1508, 3493, 4194
PshAI GACNNNNGTC 1 cut(s) 887
PshBI ATTAAT 2 cut(s) 2409, 2973
PsiI TTATAA 3 cut(s) 3779, 5580, 7260
Psp124BI GAGCTC 1 cut(s) 3916
Psp1406I AACGTT 1 cut(s) 2921
Psp5II RGGWCCY 2 cut(s) 812, 6959
PspCI CACGTG 2 cut(s) 3134, 5048
PspFI CCCAGC 1 cut(s) 7666
PspN4I GGNNCC 9 cut(s) 68, 336, 748, 813, 1197, 1710, 2318, 3359, 6960
PspPPI RGGWCCY 2 cut(s) 812, 6959
PsrI GAACNNNNNNTAC 2 cut(s) 4773, 4805
PstI CTGCAG 2 cut(s) 3421, 5386
PstNI CAGNNNCTG 5 cut(s) 2748, 3308, 3425, 5252, 7787
PsuI RGATCY 7 cut(s) 2824, 3058, 3226, 4159, 4927, 5597, 7478
PsyI GACNNNGTC 1 cut(s) 2115
PvuII CAGCTG 1 cut(s) 6446
RseI CAYNNNNRTG 3 cut(s) 780, 3387, 6890
SacI GAGCTC 1 cut(s) 3916
SacII CCGCGG 1 cut(s) 5346
SalI GTCGAC 1 cut(s) 4095
SapI GCTCTTC 1 cut(s) 7059
ScaI AGTACT 1 cut(s) 7431
SexAI ACCWGGT 1 cut(s) 7072
Sfr303I CCGCGG 1 cut(s) 5346
SgrBI CCGCGG 1 cut(s) 5346
SinI GGWCC 6 cut(s) 66, 747, 812, 2057, 2500, 6959
SmiMI CAYNNNNRTG 3 cut(s) 780, 3387, 6890
SmlI CTYRAG 7 cut(s) 2232, 2381, 2996, 4412, 4694, 7457, 7517
SmoI CTYRAG 7 cut(s) 2232, 2381, 2996, 4412, 4694, 7457, 7517
SnaBI TACGTA 1 cut(s) 5821
SphI GCATGC 3 cut(s) 1799, 4661, 5492
SseBI AGGCCT 2 cut(s) 2495, 5798
SsiI CCGC 8 cut(s) 1223, 2161, 2399, 5255, 5343, 5345, 6798, 6914
SspI AATATT 2 cut(s) 289, 5226
SstI GAGCTC 1 cut(s) 3916
StuI AGGCCT 2 cut(s) 2495, 5798
StyI CCWWGG 8 cut(s) 2496, 2559, 5397, 5770, 6917, 6962, 7437, 7807
TaqII GACCGA 2 cut(s) 427, 2770
TatI WGTACW 4 cut(s) 1932, 2623, 6139, 7429
TauI GCSGC 2 cut(s) 5258, 5345
TspGWI ACGGA 4 cut(s) 1108, 2690, 3222, 6985
Tth111I GACNNNGTC 1 cut(s) 2115
Van91I CCANNNNNTGG 4 cut(s) 1097, 1390, 2748, 3256
Vha464I CTTAAG 2 cut(s) 7457, 7517
VneI GTGCAC 1 cut(s) 637
VpaK11BI GGWCC 6 cut(s) 66, 747, 812, 2057, 2500, 6959
VspI ATTAAT 2 cut(s) 2409, 2973
XagI CCTNNNNNAGG 3 cut(s) 5774, 7460, 7695
XbaI TCTAGA 1 cut(s) 3916
XcmI CCANNNNNNNNNTGG 1 cut(s) 1098
XmiI GTMKAC 1 cut(s) 4096
ZraI GACGTC 2 cut(s) 520, 6993
ZrmI AGTACT 1 cut(s) 7431
Zsp2I ATGCAT 1 cut(s) 1944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.