FvH4_3g24320

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001486
Physical Location & Seq
Forward (+)
17401974 .. 17404612
2639 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g24320.t1

Sequence Viewer

Length: 1170 bp
ATGAGAGACCTCAATTCAATGGCTTCATCTTCTTCACCATATTTGATGAACCTCCTCCTCATATCATTATCAGTCTTCTTCGCAGGAGCATTTGGGTGTTACAGTTCAATCTTCAGCTTTGGTGATTCGATTACTGATACTGGCAACTTATATATCAGCTCCAAGAATAGTTCTTTCCACTTCTTCTTCCCTCCATATGGGGAGACCTACTTTCATCACCCCACCGGACGATGCTCCGATGGTCGCTTGATCATAGACTTCATCGCTGAGTTTCTGGGACTTCCATATGTTAGACCTTACACAGAAGTCAAGAGCAATGCAAGTCCTCAAAATATCGAAGGGGGAGTGAATTTTGCGGTTATAGGAGCCACGGCGATGGATGCTGAGTTTCTTGCTGCAATGGGAGATGAGAATGATTATACAAACAGCTCGCTAGGAACTCAACTGCATTGGTTTAAGGAATTGCTACCTTCTTTGTGCAACACGTCTTCAAACTGCAATAAGCTGCTCTCCAGCTCCCTAGTTCTTGTGGGAGAGATTGGAAGCAACGATTACAACGATGCGTTGTTGGCAGGGAAAAATATAGAACAGGTTCAGACATATGTACCATTGGTGATAACAGAAATCTCTTCAGCCATCAATGAGTTAATTGAGCTTGGGGCAGTTACACTTGTGGTACCTGGAAACTTTCCAATCGGTTGCCTTCCTATTTATCTTACAAAATTTCAGACTTCAGATAAAGGCCAGTACAACTCTTCAACAGGTTGTCTGAATTGGTTAAACAAGTTTGCTGAATATCACAATGACCAGCTTCAAATAGAACTAAGTCGGATTCAAAGGCTTCACCCCAATATCAACATCATTTATGCAGATTATTACAATGCTGTGATGCATTTCTATCATTCTCCAGATCAATTCGGATTTATTGGGGGAACAGTCAAAGCATGTTGTGGAGGCGGAGGACCATACAATTACAACTCATCAGCACAATGTGGTTCTATAGAGGCAAGTGCTTGTGAAAACCCTGCAGAGTTTATAAACTGGGATGGCATACATTTTACTGAAGCAGCATATAGGTGGATAGTCAAAGTTTTATTTAAAGGCAAATACAACGTTCCCGGTATTAGTAGATTAGTTCCTTGTGTATTTCATAAGAGTAGTGACAACTGA

Protein Analysis

390

Amino Acids

42.83

Weight (kDa)

5.14

Isoelectric Point (pI)

43.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 37 - 363 1.7e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1037
Acc65I GGTACC 1 cut(s) 676
AccB1I GGYRCC 1 cut(s) 676
AciI CCGC 2 cut(s) 356, 957
AclI AACGTT 1 cut(s) 1113
AcsI RAATTY 2 cut(s) 349, 722
AcuI CTGAAG 4 cut(s) 97, 615, 717, 1083
AdeI CACNNNGTG 1 cut(s) 992
AfaI GTAC 3 cut(s) 606, 678, 749
AfiI CCNNNNNNNGG 1 cut(s) 197
AflIII ACRYGT 1 cut(s) 483
AgsI TTSAA 6 cut(s) 18, 108, 492, 759, 815, 836
AjiI CACGTC 1 cut(s) 486
AjnI CCWGG 1 cut(s) 679
AluBI AGCT 7 cut(s) 117, 159, 429, 505, 516, 655, 811
AluI AGCT 7 cut(s) 117, 159, 429, 505, 516, 655, 811
Alw26I GTCTC 1 cut(s) 197
AoxI GGCC 1 cut(s) 742
ApeKI GCWGC 3 cut(s) 395, 505, 1067
ApoI RAATTY 2 cut(s) 349, 722
Asp700I GAANNNNTTC 1 cut(s) 591
Asp718I GGTACC 1 cut(s) 676
AspS9I GGNCC 1 cut(s) 962
AsuC2I CCSGG 1 cut(s) 1119
AsuHPI GGTGA 5 cut(s) 27, 134, 209, 625, 836
AvaII GGWCC 1 cut(s) 962
BaeI ACNNNNGTAYC 2 cut(s) 588, 621
BanI GGYRCC 1 cut(s) 676
BbsI GAAGAC 2 cut(s) 67, 480
BbvI GCAGC 3 cut(s) 382, 492, 1079
BccI CCATC 4 cut(s) 233, 370, 644, 1040
BceAI ACGGC 1 cut(s) 387
BciT130I CCWGG 1 cut(s) 681
BclI TGATCA 1 cut(s) 249
BcnI CCSGG 1 cut(s) 1119
BcoDI GTCTC 1 cut(s) 197
BfaI CTAG 2 cut(s) 434, 521
BfmI CTRYAG 2 cut(s) 999, 1026
BisI GCNGC 3 cut(s) 396, 506, 1068
BlsI GCNGC 3 cut(s) 397, 507, 1069
Bme1390I CCNGG 2 cut(s) 681, 1119
Bme18I GGWCC 1 cut(s) 962
BmgBI CACGTC 1 cut(s) 486
BmgT120I GGNCC 1 cut(s) 962
BmiI GGNNCC 2 cut(s) 367, 678
BmrFI CCNGG 2 cut(s) 681, 1119
BmrI ACTGGG 1 cut(s) 1051
BmsI GCATC 4 cut(s) 221, 370, 550, 879
BmuI ACTGGG 1 cut(s) 1051
BpiI GAAGAC 2 cut(s) 67, 480
BpmI CTGGAG 2 cut(s) 496, 891
BpuMI CCSGG 1 cut(s) 1119
BsaI GGTCTC 1 cut(s) 197
BsaJI CCNNGG 1 cut(s) 369
BsaWI WCCGGW 1 cut(s) 224
Bsc4I CCNNNNNNNGG 1 cut(s) 197
Bse1I ACTGG 3 cut(s) 145, 745, 1046
Bse3DI GCAATG 2 cut(s) 322, 405
BseBI CCWGG 1 cut(s) 681
BseDI CCNNGG 1 cut(s) 369
BseGI GGATG 2 cut(s) 385, 1051
BseLI CCNNNNNNNGG 1 cut(s) 197
BseMI GCAATG 2 cut(s) 322, 405
BseMII CTCAG 2 cut(s) 258, 375
BseNI ACTGG 3 cut(s) 145, 745, 1046
BseRI GAGGAG 2 cut(s) 44, 47
BseXI GCAGC 3 cut(s) 382, 492, 1079
BshFI GGCC 1 cut(s) 744
BshNI GGYRCC 1 cut(s) 676
BsiSI CCGG 2 cut(s) 225, 1119
BslFI GGGAC 1 cut(s) 291
BslI CCNNNNNNNGG 1 cut(s) 197
BsmAI GTCTC 1 cut(s) 197
BsmFI GGGAC 1 cut(s) 291
BsnI GGCC 1 cut(s) 744
Bso31I GGTCTC 1 cut(s) 197
Bsp143I GATC 2 cut(s) 249, 910
BspACI CCGC 2 cut(s) 356, 957
BspANI GGCC 1 cut(s) 744
BspCNI CTCAG 2 cut(s) 259, 376
BspLI GGNNCC 2 cut(s) 367, 678
BspMAI CTGCAG 1 cut(s) 1030
BspT107I GGYRCC 1 cut(s) 676
BspTNI GGTCTC 1 cut(s) 197
BsrDI GCAATG 2 cut(s) 322, 405
BsrI ACTGG 3 cut(s) 145, 745, 1046
BssECI CCNNGG 1 cut(s) 369
BssMI GATC 2 cut(s) 249, 910
Bst2UI CCWGG 1 cut(s) 681
Bst4CI ACNGT 2 cut(s) 104, 937
Bst6I CTCTTC 2 cut(s) 634, 760
BstC8I GCNNGC 1 cut(s) 431
BstDEI CTNAG 3 cut(s) 267, 384, 824
BstDSI CCRYGG 1 cut(s) 369
BstF5I GGATG 2 cut(s) 385, 1051
BstKTI GATC 2 cut(s) 252, 913
BstMAI GTCTC 1 cut(s) 197
BstMBI GATC 2 cut(s) 249, 910
BstMWI GCNNNNNNNGC 2 cut(s) 380, 569
BstNI CCWGG 1 cut(s) 681
BstNSI RCATGY 1 cut(s) 948
BstSCI CCNGG 2 cut(s) 679, 1117
BstSFI CTRYAG 2 cut(s) 999, 1026
BstV1I GCAGC 3 cut(s) 382, 492, 1079
BstV2I GAAGAC 2 cut(s) 67, 480
BstXI CCANNNNNNTGG 1 cut(s) 376
BsuRI GGCC 1 cut(s) 744
BtgI CCRYGG 1 cut(s) 369
BtgZI GCGATG 2 cut(s) 247, 389
BtrI CACGTC 1 cut(s) 486
BtsCI GGATG 2 cut(s) 385, 1051
Cac8I GCNNGC 1 cut(s) 431
Cfr13I GGNCC 1 cut(s) 962
Csp6I GTAC 3 cut(s) 605, 677, 748
CviAII CATG 1 cut(s) 945
CviQI GTAC 3 cut(s) 605, 677, 748
DdeI CTNAG 3 cut(s) 267, 384, 824
DpnI GATC 2 cut(s) 251, 912
DpnII GATC 2 cut(s) 249, 910
DraI TTTAAA 1 cut(s) 1099
DraIII CACNNNGTG 1 cut(s) 992
Eam1104I CTCTTC 2 cut(s) 634, 760
EarI CTCTTC 2 cut(s) 634, 760
EciI GGCGGA 1 cut(s) 972
Eco31I GGTCTC 1 cut(s) 197
Eco47I GGWCC 1 cut(s) 962
Eco57I CTGAAG 4 cut(s) 97, 615, 717, 1083
EcoRII CCWGG 1 cut(s) 679
EcoT22I ATGCAT 1 cut(s) 894
FaeI CATG 1 cut(s) 948
FaqI GGGAC 1 cut(s) 291
FatI CATG 1 cut(s) 944
FauNDI CATATG 3 cut(s) 196, 286, 601
FbaI TGATCA 1 cut(s) 249
Fnu4HI GCNGC 3 cut(s) 396, 506, 1068
FokI GGATG 2 cut(s) 392, 1058
Fsp4HI GCNGC 3 cut(s) 396, 506, 1068
FspBI CTAG 2 cut(s) 434, 521
GluI GCNGC 3 cut(s) 396, 506, 1068
GsuI CTGGAG 2 cut(s) 496, 891
HaeIII GGCC 1 cut(s) 744
HapII CCGG 2 cut(s) 225, 1119
Hin1II CATG 1 cut(s) 948
HinfI GANTC 2 cut(s) 125, 832
HpaII CCGG 2 cut(s) 225, 1119
HphI GGTGA 5 cut(s) 27, 134, 209, 625, 836
Hpy188I TCNGA 7 cut(s) 238, 597, 729, 736, 771, 831, 920
Hpy188III TCNNGA 2 cut(s) 310, 908
HpyAV CCTTC 3 cut(s) 332, 480, 713
HpyCH4III ACNGT 2 cut(s) 104, 937
HpyCH4IV ACGT 2 cut(s) 485, 1113
HpyCH4V TGCA 8 cut(s) 320, 398, 448, 480, 498, 869, 892, 1028
HpyF10VI GCNNNNNNNGC 2 cut(s) 380, 569
HpyF3I CTNAG 3 cut(s) 267, 384, 824
HpySE526I ACGT 2 cut(s) 485, 1113
Hsp92II CATG 1 cut(s) 948
KpnI GGTACC 1 cut(s) 680
Ksp22I TGATCA 1 cut(s) 249
Kzo9I GATC 2 cut(s) 249, 910
LmnI GCTCC 5 cut(s) 86, 164, 239, 365, 521
Lsp1109I GCAGC 3 cut(s) 382, 492, 1079
LweI GCATC 4 cut(s) 221, 370, 550, 879
MaeI CTAG 2 cut(s) 434, 521
MaeII ACGT 2 cut(s) 485, 1113
MaeIII GTNAC 3 cut(s) 98, 664, 1160
MalI GATC 2 cut(s) 251, 912
MboI GATC 2 cut(s) 249, 910
MluCI AATT 8 cut(s) 13, 349, 461, 648, 722, 772, 914, 970
MmeI TCCRAC 1 cut(s) 809
MnlI CCTC 9 cut(s) 20, 62, 65, 68, 201, 336, 947, 953, 997
Mph1103I ATGCAT 1 cut(s) 894
MroXI GAANNNNTTC 1 cut(s) 591
MseI TTAA 4 cut(s) 456, 647, 779, 1098
MslI CAYNNNNRTG 3 cut(s) 94, 374, 1075
MspI CCGG 2 cut(s) 225, 1119
MspR9I CCNGG 2 cut(s) 681, 1119
MvaI CCWGG 1 cut(s) 681
MwoI GCNNNNNNNGC 2 cut(s) 380, 569
NciI CCSGG 1 cut(s) 1119
NdeI CATATG 3 cut(s) 196, 286, 601
NdeII GATC 2 cut(s) 249, 910
NlaIII CATG 1 cut(s) 948
NlaIV GGNNCC 2 cut(s) 367, 678
NmuCI GTSAC 1 cut(s) 1160
NsiI ATGCAT 1 cut(s) 894
NspI RCATGY 1 cut(s) 948
PcsI WCGNNNNNNNCGW 1 cut(s) 555
PdmI GAANNNNTTC 1 cut(s) 591
PfeI GAWTC 2 cut(s) 125, 832
PkrI GCNGC 3 cut(s) 397, 507, 1069
PsiI TTATAA 1 cut(s) 1037
Psp1406I AACGTT 1 cut(s) 1113
Psp6I CCWGG 1 cut(s) 679
PspGI CCWGG 1 cut(s) 679
PspN4I GGNNCC 2 cut(s) 367, 678
PspPI GGNCC 1 cut(s) 962
PstI CTGCAG 1 cut(s) 1030
RsaI GTAC 3 cut(s) 606, 678, 749
RsaNI GTAC 3 cut(s) 605, 677, 748
RseI CAYNNNNRTG 3 cut(s) 94, 374, 1075
SaqAI TTAA 4 cut(s) 456, 647, 779, 1098
SatI GCNGC 3 cut(s) 396, 506, 1068
Sau3AI GATC 2 cut(s) 249, 910
Sau96I GGNCC 1 cut(s) 962
ScrFI CCNGG 2 cut(s) 681, 1119
SfaNI GCATC 4 cut(s) 221, 370, 550, 879
SfcI CTRYAG 2 cut(s) 999, 1026
SinI GGWCC 1 cut(s) 962
SmiMI CAYNNNNRTG 3 cut(s) 94, 374, 1075
Sse9I AATT 8 cut(s) 13, 349, 461, 648, 722, 772, 914, 970
SsiI CCGC 2 cut(s) 356, 957
SspMI CTAG 2 cut(s) 434, 521
StyD4I CCNGG 2 cut(s) 679, 1117
TaaI ACNGT 2 cut(s) 104, 937
TaiI ACGT 2 cut(s) 488, 1116
TaqI TCGA 2 cut(s) 128, 336
TasI AATT 8 cut(s) 13, 349, 461, 648, 722, 772, 914, 970
TatI WGTACW 1 cut(s) 747
TfiI GAWTC 2 cut(s) 125, 832
Tru1I TTAA 4 cut(s) 456, 647, 779, 1098
Tru9I TTAA 4 cut(s) 456, 647, 779, 1098
TseFI GTSAC 1 cut(s) 1160
TseI GCWGC 3 cut(s) 395, 505, 1067
Tsp45I GTSAC 1 cut(s) 1160
TspDTI ATGAA 5 cut(s) 15, 62, 203, 250, 1139
VpaK11BI GGWCC 1 cut(s) 962
XapI RAATTY 2 cut(s) 349, 722
XceI RCATGY 1 cut(s) 948
XmnI GAANNNNTTC 1 cut(s) 591
XspI CTAG 2 cut(s) 434, 521
Zsp2I ATGCAT 1 cut(s) 894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.