RLG00000031730

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001486
Physical Location & Seq
Forward (+)
7840715 .. 7842320
1606 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031730

Sequence Viewer

Length: 978 bp
ATGGGTTCATCTTCTTCACCATATTTGATCAACTTCCTCCTCATCTTACTATCAATCATCATCCCAAAAGCGCTTGGTTGCTACAGTACAATCTTCAGCTTTGGTGATTCGATTACTGATACTGGCAACTTATATATCAGCTCCAAGAACAGTTCCTTCCACTTCTTCTTCCCTCCATATGGGGAGACCTGCTTTCATCACCCCACCGGACGATGCTCCGATGGTCGCTTGATCATAGACTTCATTGCTGAGTTTCTGGGACTTCCACATGTTAGACCTTATCTTGAACTCAAGAGCAATGTAAGTGCTCAAAACTCCAAAGAGGCAGTGAATTTCTCGGTGGTAGGAGCCACAGCGCTTGATGCGGCATTTCTTGCAGCAATGGGAGATCATAATGATTATACAAACAGCTCTCTAAGAACTCAATTGGATTGGTTTAAAGAAATGCTGCCTTCTTTATGCAATACATCTTCAAATTGCAATAAACTTCTCTCCAGCTCCCTATTTCCCTATTTCTTGTGGGAGAGATTGGAGGCAACGATTACAACGATGCATTGTTGGCAGGGAAAAGAGTTAATTGAGCTTGGGGCAATGACACTTATGGTACCTGGCAACTTTCCAATCGGTTGCCTTCCCATTTATCTTACAAAATTTCAGACTTCAGATAAAAGGCACTACGACTCTTCGACAGGTTGTCTGAATTGGTTAAACAAGTTTGCTGAATATCACAATGACCAGCTTCAAATAGAACTAAGTCAGGTTCAAAGGCTTCACCCCAATGTCAACATCATTTATGCAGATTATTACAATGCTGATTTATTGGGGGAACAGTCGAAGCATGCTGTGGAGGGGGAGGACCATACCATTACAACTCATCGGCACAATGTGGCTCTCTGGAGGCAAGTGCTTGTGAAAACCCTGCACAGTTTATCAACTGGGATGGTATACACTTTACTGAAGCAGCATATAGGTGGATAG

Protein Analysis

326

Amino Acids

36.57

Weight (kDa)

6.18

Isoelectric Point (pI)

40.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 31 - 270 5.9e-14 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 197
Acc65I GGTACC 1 cut(s) 604
AccB1I GGYRCC 1 cut(s) 604
AccI GTMKAC 1 cut(s) 945
AciI CCGC 1 cut(s) 365
AcsI RAATTY 2 cut(s) 331, 650
AcuI CTGAAG 3 cut(s) 79, 645, 977
AdeI CACNNNGTG 1 cut(s) 886
AfaI GTAC 2 cut(s) 88, 606
AfeI AGCGCT 2 cut(s) 72, 357
AfiI CCNNNNNNNGG 1 cut(s) 179
AflIII ACRYGT 1 cut(s) 268
AgsI TTSAA 4 cut(s) 287, 474, 743, 764
AhdI GACNNNNNGTC 1 cut(s) 693
AjnI CCWGG 1 cut(s) 607
AluBI AGCT 6 cut(s) 99, 141, 411, 498, 583, 739
AluI AGCT 6 cut(s) 99, 141, 411, 498, 583, 739
Alw21I GWGCWC 1 cut(s) 310
Alw26I GTCTC 1 cut(s) 179
Aor51HI AGCGCT 2 cut(s) 72, 357
ApeKI GCWGC 3 cut(s) 377, 448, 961
ApoI RAATTY 2 cut(s) 331, 650
Asp718I GGTACC 1 cut(s) 604
AspLEI GCGC 2 cut(s) 73, 358
AspS9I GGNCC 1 cut(s) 856
AsuHPI GGTGA 4 cut(s) 9, 116, 191, 764
AvaII GGWCC 1 cut(s) 856
BanI GGYRCC 1 cut(s) 604
Bbv12I GWGCWC 1 cut(s) 310
BbvI GCAGC 3 cut(s) 389, 435, 973
BccI CCATC 2 cut(s) 215, 934
BciT130I CCWGG 1 cut(s) 609
BclI TGATCA 2 cut(s) 27, 231
BcoDI GTCTC 1 cut(s) 179
BfmI CTRYAG 1 cut(s) 82
BfoI RGCGCY 2 cut(s) 74, 359
BfuAI ACCTGC 1 cut(s) 197
BisI GCNGC 4 cut(s) 366, 378, 449, 962
BlsI GCNGC 4 cut(s) 367, 379, 450, 963
Bme1390I CCNGG 1 cut(s) 609
Bme18I GGWCC 1 cut(s) 856
BmeRI GACNNNNNGTC 1 cut(s) 693
BmgT120I GGNCC 1 cut(s) 856
BmiI GGNNCC 2 cut(s) 349, 606
BmrFI CCNGG 1 cut(s) 609
BmrI ACTGGG 1 cut(s) 945
BmsI GCATC 3 cut(s) 203, 352, 540
BmuI ACTGGG 1 cut(s) 945
BpmI CTGGAG 2 cut(s) 478, 916
BpuEI CTTGAG 1 cut(s) 275
BsaI GGTCTC 1 cut(s) 179
BsaWI WCCGGW 1 cut(s) 206
Bsc4I CCNNNNNNNGG 1 cut(s) 179
Bse1I ACTGG 2 cut(s) 127, 940
Bse3DI GCAATG 4 cut(s) 243, 304, 387, 597
BseBI CCWGG 1 cut(s) 609
BseGI GGATG 2 cut(s) 60, 945
BseLI CCNNNNNNNGG 1 cut(s) 179
BseMI GCAATG 4 cut(s) 243, 304, 387, 597
BseMII CTCAG 1 cut(s) 240
BseNI ACTGG 2 cut(s) 127, 940
BseRI GAGGAG 1 cut(s) 29
BseXI GCAGC 3 cut(s) 389, 435, 973
BsgI GTGCAG 1 cut(s) 905
BshNI GGYRCC 1 cut(s) 604
BsiHKAI GWGCWC 1 cut(s) 310
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 1 cut(s) 273
BslI CCNNNNNNNGG 1 cut(s) 179
BsmAI GTCTC 1 cut(s) 179
BsmFI GGGAC 1 cut(s) 273
Bso31I GGTCTC 1 cut(s) 179
Bsp1286I GDGCHC 1 cut(s) 310
Bsp143I GATC 3 cut(s) 27, 231, 388
BspACI CCGC 1 cut(s) 365
BspCNI CTCAG 1 cut(s) 241
BspLI GGNNCC 2 cut(s) 349, 606
BspMI ACCTGC 1 cut(s) 197
BspT107I GGYRCC 1 cut(s) 604
BspTNI GGTCTC 1 cut(s) 179
BsrDI GCAATG 4 cut(s) 243, 304, 387, 597
BsrI ACTGG 2 cut(s) 127, 940
BssMI GATC 3 cut(s) 27, 231, 388
BssNAI GTATAC 1 cut(s) 946
Bst1107I GTATAC 1 cut(s) 946
Bst2UI CCWGG 1 cut(s) 609
Bst4CI ACNGT 4 cut(s) 86, 152, 831, 926
Bst6I CTCTTC 1 cut(s) 688
BstAPI GCANNNNNTGC 1 cut(s) 374
BstC8I GCNNGC 1 cut(s) 840
BstDEI CTNAG 3 cut(s) 249, 416, 752
BstF5I GGATG 2 cut(s) 60, 945
BstH2I RGCGCY 2 cut(s) 74, 359
BstHHI GCGC 2 cut(s) 73, 358
BstKTI GATC 3 cut(s) 30, 234, 391
BstMAI GTCTC 1 cut(s) 179
BstMBI GATC 3 cut(s) 27, 231, 388
BstMWI GCNNNNNNNGC 3 cut(s) 362, 374, 559
BstNI CCWGG 1 cut(s) 609
BstNSI RCATGY 2 cut(s) 272, 842
BstSCI CCNGG 1 cut(s) 607
BstSFI CTRYAG 1 cut(s) 82
BstV1I GCAGC 3 cut(s) 389, 435, 973
BstZ17I GTATAC 1 cut(s) 946
BtsCI GGATG 2 cut(s) 60, 945
BtsI GCAGTG 1 cut(s) 333
BtsIMutI CAGTG 1 cut(s) 333
BveI ACCTGC 1 cut(s) 197
Cac8I GCNNGC 1 cut(s) 840
CfoI GCGC 2 cut(s) 73, 358
Cfr13I GGNCC 1 cut(s) 856
Csp6I GTAC 2 cut(s) 87, 605
CspCI CAANNNNNGTGG 2 cut(s) 340, 375
CviAII CATG 2 cut(s) 269, 839
CviJI RGCY 9 cut(s) 99, 141, 350, 411, 498, 583, 739, 769, 890
CviKI_1 RGCY 9 cut(s) 99, 141, 350, 411, 498, 583, 739, 769, 890
CviQI GTAC 2 cut(s) 87, 605
DdeI CTNAG 3 cut(s) 249, 416, 752
DpnI GATC 3 cut(s) 29, 233, 390
DpnII GATC 3 cut(s) 27, 231, 388
DraI TTTAAA 1 cut(s) 439
DraIII CACNNNGTG 1 cut(s) 886
DriI GACNNNNNGTC 1 cut(s) 693
Eam1104I CTCTTC 1 cut(s) 688
Eam1105I GACNNNNNGTC 1 cut(s) 693
EarI CTCTTC 1 cut(s) 688
Eco31I GGTCTC 1 cut(s) 179
Eco47I GGWCC 1 cut(s) 856
Eco47III AGCGCT 2 cut(s) 72, 357
Eco57I CTGAAG 3 cut(s) 79, 645, 977
EcoRII CCWGG 1 cut(s) 607
EcoT22I ATGCAT 1 cut(s) 555
FaeI CATG 2 cut(s) 272, 842
FaqI GGGAC 1 cut(s) 273
FatI CATG 2 cut(s) 268, 838
FauNDI CATATG 1 cut(s) 178
FbaI TGATCA 2 cut(s) 27, 231
FblI GTMKAC 1 cut(s) 945
Fnu4HI GCNGC 4 cut(s) 366, 378, 449, 962
FokI GGATG 2 cut(s) 47, 952
Fsp4HI GCNGC 4 cut(s) 366, 378, 449, 962
GlaI GCGC 2 cut(s) 72, 357
GluI GCNGC 4 cut(s) 366, 378, 449, 962
GsuI CTGGAG 2 cut(s) 478, 916
HaeII RGCGCY 2 cut(s) 74, 359
HapII CCGG 1 cut(s) 207
HhaI GCGC 2 cut(s) 73, 358
Hin1II CATG 2 cut(s) 272, 842
Hin6I GCGC 2 cut(s) 71, 356
HinP1I GCGC 2 cut(s) 71, 356
HincII GTYRAC 1 cut(s) 784
HindII GTYRAC 1 cut(s) 784
HinfI GANTC 2 cut(s) 107, 680
HpaII CCGG 1 cut(s) 207
HphI GGTGA 4 cut(s) 9, 116, 191, 764
Hpy166II GTNNAC 2 cut(s) 784, 946
Hpy188I TCNGA 4 cut(s) 220, 657, 664, 699
Hpy188III TCNNGA 3 cut(s) 284, 292, 895
Hpy8I GTNNAC 2 cut(s) 784, 946
HpyAV CCTTC 3 cut(s) 166, 462, 641
HpyCH4III ACNGT 4 cut(s) 86, 152, 831, 926
HpyCH4V TGCA 6 cut(s) 377, 462, 480, 553, 797, 922
HpyF10VI GCNNNNNNNGC 3 cut(s) 362, 374, 559
HpyF3I CTNAG 3 cut(s) 249, 416, 752
Hsp92II CATG 2 cut(s) 272, 842
HspAI GCGC 2 cut(s) 71, 356
KpnI GGTACC 1 cut(s) 608
Ksp22I TGATCA 2 cut(s) 27, 231
Kzo9I GATC 3 cut(s) 27, 231, 388
LmnI GCTCC 4 cut(s) 146, 221, 347, 503
Lsp1109I GCAGC 3 cut(s) 389, 435, 973
LweI GCATC 3 cut(s) 203, 352, 540
MalI GATC 3 cut(s) 29, 233, 390
MboI GATC 3 cut(s) 27, 231, 388
MboII GAAGA 7 cut(s) 3, 6, 85, 157, 160, 462, 675
MfeI CAATTG 1 cut(s) 425
MhlI GDGCHC 1 cut(s) 310
MluCI AATT 6 cut(s) 331, 425, 475, 576, 650, 700
MlyI GAGTC 1 cut(s) 674
MnlI CCTC 8 cut(s) 47, 50, 183, 316, 526, 841, 847, 891
Mph1103I ATGCAT 1 cut(s) 555
MseI TTAA 3 cut(s) 438, 575, 707
MslI CAYNNNNRTG 2 cut(s) 777, 969
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 1 cut(s) 609
MunI CAATTG 1 cut(s) 425
MvaI CCWGG 1 cut(s) 609
MwoI GCNNNNNNNGC 3 cut(s) 362, 374, 559
NdeI CATATG 1 cut(s) 178
NdeII GATC 3 cut(s) 27, 231, 388
NlaIII CATG 2 cut(s) 272, 842
NlaIV GGNNCC 2 cut(s) 349, 606
NsiI ATGCAT 1 cut(s) 555
NspI RCATGY 2 cut(s) 272, 842
PaeI GCATGC 1 cut(s) 842
PciI ACATGT 1 cut(s) 268
PcsI WCGNNNNNNNCGW 1 cut(s) 545
PfeI GAWTC 1 cut(s) 107
PkrI GCNGC 4 cut(s) 367, 379, 450, 963
PleI GAGTC 1 cut(s) 674
PpsI GAGTC 1 cut(s) 674
PscI ACATGT 1 cut(s) 268
Psp6I CCWGG 1 cut(s) 607
PspGI CCWGG 1 cut(s) 607
PspN4I GGNNCC 2 cut(s) 349, 606
PspPI GGNCC 1 cut(s) 856
RsaI GTAC 2 cut(s) 88, 606
RsaNI GTAC 2 cut(s) 87, 605
RseI CAYNNNNRTG 2 cut(s) 777, 969
SaqAI TTAA 3 cut(s) 438, 575, 707
SatI GCNGC 4 cut(s) 366, 378, 449, 962
Sau3AI GATC 3 cut(s) 27, 231, 388
Sau96I GGNCC 1 cut(s) 856
SchI GAGTC 1 cut(s) 674
ScrFI CCNGG 1 cut(s) 609
SduI GDGCHC 1 cut(s) 310
SfaNI GCATC 3 cut(s) 203, 352, 540
SfcI CTRYAG 1 cut(s) 82
SinI GGWCC 1 cut(s) 856
SmiMI CAYNNNNRTG 2 cut(s) 777, 969
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
SphI GCATGC 1 cut(s) 842
Sse9I AATT 6 cut(s) 331, 425, 475, 576, 650, 700
SsiI CCGC 1 cut(s) 365
StyD4I CCNGG 1 cut(s) 607
TaaI ACNGT 4 cut(s) 86, 152, 831, 926
TaqI TCGA 3 cut(s) 110, 686, 833
TasI AATT 6 cut(s) 331, 425, 475, 576, 650, 700
TatI WGTACW 1 cut(s) 86
TauI GCSGC 1 cut(s) 368
TfiI GAWTC 1 cut(s) 107
Tru1I TTAA 3 cut(s) 438, 575, 707
Tru9I TTAA 3 cut(s) 438, 575, 707
TscAI CASTG 1 cut(s) 333
TseI GCWGC 3 cut(s) 377, 448, 961
TspDTI ATGAA 2 cut(s) 185, 232
TspRI CASTG 1 cut(s) 333
VpaK11BI GGWCC 1 cut(s) 856
XapI RAATTY 2 cut(s) 331, 650
XceI RCATGY 2 cut(s) 272, 842
XmiI GTMKAC 1 cut(s) 945
Zsp2I ATGCAT 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.