Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0001486

Physical Location & Seq

Genomic Coordinates Reverse (-)

37350769 .. 37354417

Gene Structure 3649 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1283200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1149 bp

ATGGCTTGGTATTATCGGGCAAGTTTGACCATGCACGTCCTCCTTCTCCTAACAGTTGTGATCATCCCGTACGCGAATTGTGGCTACACATCTATCATTGGGTTTGGGGATTCACTTGCTGATACTGGCAACGAATACATCTATGCCCTTGACCACCATAAGGGTCCGTACCACTTCCTCTTCCCTCCAAATGGGGAGACCTACTTTCATCACCCCACCGGGCGCTGCTGCGACGGTCGTTTAATGATAGACCTCATTGCTGAATCTATGGGGCTTCCACTAGTACGACCTTATCTTGAAATCCTGAACGTCAATGAAACGGTGCGCAACCTTGACGAGGGTGTGAATTTTGCAGTTGTTGGAGCCACTGCACTTGACACTGACTTTCTTGACCCAATGGGACGTTATGATATATTTACAAACTTCTCTCTAAGAATTCAAGTGGACTGGTTCAAGGGAATGCTATCACCTTTATGCAATACATCTTCAGACTGCAGGAATCTCCTTTCAAGTTCTCTTATTGTTATGGGACCGATTGCAGGCAACGATTACAACTGTCCGTTTTTCGCAGGAGAGAGTTTGGAAATTGTTAAAACATATGTTCCGTTCGTGATAGAAGCAATGGCTTCAGCCATCAATGAGTTGATTCAGCATGGGGCATCGACACTTTTGGTACCAGGCAACGCTCCAATTGGTTGCATTCCTTGTTATCTTACCTTATATGAGGGTTCAGATGAGAGTGAATATGACCCTTTAACTGGCTGTCTTACCTGGTTAAACAAGTTTTCTGAGTATCACAATGAGCTACTTAAGACAAAGCTAAGTCAGATTCGAAGCCTTCATCCTCATGTCAACATCATTTATGGAGATCTTTACAATGCTGAGCTACAATTGTATCAGTCTCCAGAACATTTTGGATTTACCGAGAATACTTCCAGAGCATGCTGTGGAGGAGGAGGGACGTACAATTTCAACTTATCAGCACTATGTGGTCTTCCAGGATCACGTGCTTGTGCAAACCCTGCAAAGTCTATCAACTGGGACGGTCGGCACACTACTGAAGCAGCAAACCAGTGGATAGCCAAGGCCTTATTAAATGGAATTTACACCATTCCCCACATTAGTACCTCCTCTGTTTCACAACAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00901 ko01110 map00901 map01110

Pfam Domains

Protein Families

Lipase_GDSL

Protein Analysis

383

Amino Acids

42.22

Weight (kDa)

5.13

Isoelectric Point (pI)

35.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipase_GDSL	PF00657	32 - 364	3.4e-24	GDSL-like Lipase/Acylhydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001486)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G28570 AT1G28570 AT1G28570 AT1G28580 AT1G28580 AT1G28590 AT1G28600 AT1G28600 AT1G28610 AT1G28610 AT1G28640 AT1G28650 AT1G28660 AT1G28660 AT1G28670 AT1G31550 AT1G31550 AT2G27360
fragaria_vesca	FvH4_3g24320 FvH4_3g24320
malus_domestica	MD05G1303900.v1.1 MD05G1304000.v1.1 MD10G1283200.v1.1 MD10G1283400.v1.1
prunus_persica	Prupe.4G058600_v2.0.a1 Prupe.4G058700_v2.0.a1 Prupe.4G058800_v2.0.a1 Prupe.4G058900_v2.0.a1
pyrus_communis	pycom05g28080 pycom05g28100 pycom05g28110 pycom05g28120 pycom05g28130 pycom05g28140 pycom10g23610 pycom10g23620 pycom10g23630 pycom10g23640 pycom16g08500
rosa_chinensis	RchiOBHm_Chr5g0010201 RchiOBHm_Chr5g0010241
rosa_laevigata	RLG00000031730
rosa_multiflora	Rmu_co8023010.1_g000001 Rmu_co8377757.1_g000001
rosa_roxburghii	Rroxscaffold_1G00066020
rosa_rugosa	Rorug04G0449600
rosa_samantha	Rh5AG080600 Rh5BG075200 Rh5CG088900 Rh5DG076100
rosa_wichuraiana	Rw5G007470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	326
Acc65I	GGTACC	1 cut(s)	673
AccB1I	GGYRCC	1 cut(s)	673
AccII	CGCG	1 cut(s)	74
AclWI	GGATC	1 cut(s)	1009
AcsI	RAATTY	3 cut(s)	346, 435, 1101
AcuI	CTGAAG	3 cut(s)	471, 612, 1080
AcvI	CACGTG	1 cut(s)	1007
AdeI	CACNNNGTG	1 cut(s)	989
AfaI	GTAC	6 cut(s)	71, 170, 285, 675, 965, 1126
AfiI	CCNNNNNNNGG	5 cut(s)	160, 191, 337, 539, 758
AflII	CTTAAG	1 cut(s)	809
AgsI	TTSAA	5 cut(s)	299, 440, 454, 510, 973
AhlI	ACTAGT	1 cut(s)	280
AjiI	CACGTC	1 cut(s)	37
AjnI	CCWGG	3 cut(s)	676, 770, 997
AleI	CACNNNNGTG	1 cut(s)	1144
AluBI	AGCT	3 cut(s)	805, 820, 886
AluI	AGCT	3 cut(s)	805, 820, 886
Alw26I	GTCTC	2 cut(s)	191, 906
AlwI	GGATC	1 cut(s)	1009
AoxI	GGCC	1 cut(s)	1086
ApeKI	GCWGC	3 cut(s)	225, 228, 1064
ApoI	RAATTY	3 cut(s)	346, 435, 1101
Asp718I	GGTACC	1 cut(s)	673
AspLEI	GCGC	2 cut(s)	225, 327
AspS9I	GGNCC	2 cut(s)	164, 530
AsuC2I	CCSGG	1 cut(s)	220
AsuHPI	GGTGA	2 cut(s)	203, 459
AsuII	TTCGAA	1 cut(s)	832
AvaII	GGWCC	2 cut(s)	164, 530
BaeI	ACNNNNGTAYC	4 cut(s)	878, 911, 1108, 1141
BanI	GGYRCC	1 cut(s)	673
BbrPI	CACGTG	1 cut(s)	1007
BbsI	GAAGAC	1 cut(s)	986
BbvI	GCAGC	3 cut(s)	212, 215, 1076
BccI	CCATC	1 cut(s)	641
BciT130I	CCWGG	3 cut(s)	678, 772, 999
BclI	TGATCA	1 cut(s)	60
BcnI	CCSGG	1 cut(s)	220
BcoDI	GTCTC	2 cut(s)	191, 906
BcuI	ACTAGT	1 cut(s)	280
BfaI	CTAG	1 cut(s)	281
BfmI	CTRYAG	1 cut(s)	493
BfoI	RGCGCY	1 cut(s)	226
BfrI	CTTAAG	1 cut(s)	809
BglII	AGATCT	1 cut(s)	868
BisI	GCNGC	3 cut(s)	226, 229, 1065
BlpI	GCTNAGC	1 cut(s)	882
BlsI	GCNGC	3 cut(s)	227, 230, 1066
Bme1390I	CCNGG	4 cut(s)	220, 678, 772, 999
Bme18I	GGWCC	2 cut(s)	164, 530
BmgBI	CACGTC	1 cut(s)	37
BmgT120I	GGNCC	2 cut(s)	164, 530
BmiI	GGNNCC	4 cut(s)	165, 364, 531, 675
BmrFI	CCNGG	4 cut(s)	220, 678, 772, 999
BmrI	ACTGGG	1 cut(s)	1048
BmsI	GCATC	1 cut(s)	668
BmuI	ACTGGG	1 cut(s)	1048
BpiI	GAAGAC	1 cut(s)	986
BpmI	CTGGAG	1 cut(s)	888
Bpu1102I	GCTNAGC	1 cut(s)	882
Bpu14I	TTCGAA	1 cut(s)	832
BpuMI	CCSGG	1 cut(s)	220
BsaAI	YACGTR	1 cut(s)	1007
BsaI	GGTCTC	1 cut(s)	191
BsaJI	CCNNGG	1 cut(s)	1083
Bsc4I	CCNNNNNNNGG	5 cut(s)	160, 191, 337, 539, 758
Bse1I	ACTGG	5 cut(s)	130, 452, 763, 1043, 1072
Bse3DI	GCAATG	2 cut(s)	255, 627
BseBI	CCWGG	3 cut(s)	678, 772, 999
BseDI	CCNNGG	1 cut(s)	1083
BseGI	GGATG	2 cut(s)	63, 841
BseLI	CCNNNNNNNGG	5 cut(s)	160, 191, 337, 539, 758
BseMI	GCAATG	2 cut(s)	255, 627
BseMII	CTCAG	2 cut(s)	780, 873
BseNI	ACTGG	5 cut(s)	130, 452, 763, 1043, 1072
BseRI	GAGGAG	3 cut(s)	966, 969, 1120
BseXI	GCAGC	3 cut(s)	212, 215, 1076
BsgI	GTGCAG	1 cut(s)	354
Bsh1236I	CGCG	1 cut(s)	74
Bsh1285I	CGRYCG	2 cut(s)	238, 1048
BshFI	GGCC	1 cut(s)	1088
BshNI	GGYRCC	1 cut(s)	673
BsiEI	CGRYCG	2 cut(s)	238, 1048
BsiSI	CCGG	1 cut(s)	219
BsiWI	CGTACG	1 cut(s)	69
BslFI	GGGAC	4 cut(s)	414, 543, 973, 1055
BslI	CCNNNNNNNGG	5 cut(s)	160, 191, 337, 539, 758
BsmAI	GTCTC	2 cut(s)	191, 906
BsmFI	GGGAC	4 cut(s)	414, 543, 973, 1055
BsmI	GAATGC	2 cut(s)	465, 699
BsnI	GGCC	1 cut(s)	1088
Bso31I	GGTCTC	1 cut(s)	191
Bsp119I	TTCGAA	1 cut(s)	832
Bsp143I	GATC	3 cut(s)	60, 868, 1001
Bsp1720I	GCTNAGC	1 cut(s)	882
BspANI	GGCC	1 cut(s)	1088
BspCNI	CTCAG	2 cut(s)	781, 874
BspFNI	CGCG	1 cut(s)	74
BspLI	GGNNCC	4 cut(s)	165, 364, 531, 675
BspMAI	CTGCAG	1 cut(s)	497
BspPI	GGATC	1 cut(s)	1009
BspT104I	TTCGAA	1 cut(s)	832
BspT107I	GGYRCC	1 cut(s)	673
BspTI	CTTAAG	1 cut(s)	809
BspTNI	GGTCTC	1 cut(s)	191
BsrDI	GCAATG	2 cut(s)	255, 627
BsrI	ACTGG	5 cut(s)	130, 452, 763, 1043, 1072
BssECI	CCNNGG	1 cut(s)	1083
BssMI	GATC	3 cut(s)	60, 868, 1001
BssT1I	CCWWGG	1 cut(s)	1083
Bst2UI	CCWGG	3 cut(s)	678, 772, 999
Bst4CI	ACNGT	6 cut(s)	55, 236, 322, 557, 1046, 1146
Bst6I	CTCTTC	1 cut(s)	185
BstAFI	CTTAAG	1 cut(s)	809
BstAPI	GCANNNNNTGC	1 cut(s)	1022
BstBAI	YACGTR	1 cut(s)	1007
BstBI	TTCGAA	1 cut(s)	832
BstC8I	GCNNGC	2 cut(s)	541, 943
BstDEI	CTNAG	4 cut(s)	431, 789, 821, 882
BstENI	CCTNNNNNAGG	1 cut(s)	335
BstF5I	GGATG	2 cut(s)	63, 841
BstFNI	CGCG	1 cut(s)	74
BstH2I	RGCGCY	1 cut(s)	226
BstHHI	GCGC	2 cut(s)	225, 327
BstKTI	GATC	3 cut(s)	63, 871, 1004
BstMAI	GTCTC	2 cut(s)	191, 906
BstMBI	GATC	3 cut(s)	60, 868, 1001
BstMCI	CGRYCG	2 cut(s)	238, 1048
BstMWI	GCNNNNNNNGC	1 cut(s)	1022
BstNI	CCWGG	3 cut(s)	678, 772, 999
BstNSI	RCATGY	1 cut(s)	945
BstSCI	CCNGG	4 cut(s)	218, 676, 770, 997
BstSFI	CTRYAG	1 cut(s)	493
BstUI	CGCG	1 cut(s)	74
BstV1I	GCAGC	3 cut(s)	212, 215, 1076
BstV2I	GAAGAC	1 cut(s)	986
BstX2I	RGATCY	1 cut(s)	868
BstYI	RGATCY	1 cut(s)	868
BsuRI	GGCC	1 cut(s)	1088
BtrI	CACGTC	1 cut(s)	37
BtsCI	GGATG	2 cut(s)	63, 841
BtsI	GCAGTG	1 cut(s)	366
BtsIMutI	CAGTG	3 cut(s)	366, 378, 1079
Cac8I	GCNNGC	2 cut(s)	541, 943
CfoI	GCGC	2 cut(s)	225, 327
Cfr13I	GGNCC	2 cut(s)	164, 530
CsiI	ACCWGGT	1 cut(s)	770
Csp6I	GTAC	6 cut(s)	70, 169, 284, 674, 964, 1125
CspCI	CAANNNNNGTGG	2 cut(s)	355, 390
CviAII	CATG	4 cut(s)	31, 653, 848, 942
CviQI	GTAC	6 cut(s)	70, 169, 284, 674, 964, 1125
DdeI	CTNAG	4 cut(s)	431, 789, 821, 882
DpnI	GATC	3 cut(s)	62, 870, 1003
DpnII	GATC	3 cut(s)	60, 868, 1001
DraIII	CACNNNGTG	1 cut(s)	989
Eam1104I	CTCTTC	1 cut(s)	185
EarI	CTCTTC	1 cut(s)	185
Eco130I	CCWWGG	1 cut(s)	1083
Eco147I	AGGCCT	1 cut(s)	1088
Eco31I	GGTCTC	1 cut(s)	191
Eco47I	GGWCC	2 cut(s)	164, 530
Eco57I	CTGAAG	3 cut(s)	471, 612, 1080
Eco72I	CACGTG	1 cut(s)	1007
EcoNI	CCTNNNNNAGG	1 cut(s)	335
EcoRI	GAATTC	1 cut(s)	435
EcoRII	CCWGG	3 cut(s)	676, 770, 997
EcoT14I	CCWWGG	1 cut(s)	1083
ErhI	CCWWGG	1 cut(s)	1083
FaeI	CATG	4 cut(s)	34, 656, 851, 945
FaqI	GGGAC	4 cut(s)	414, 543, 973, 1055
FatI	CATG	4 cut(s)	30, 652, 847, 941
FauNDI	CATATG	1 cut(s)	598
FbaI	TGATCA	1 cut(s)	60
Fnu4HI	GCNGC	3 cut(s)	226, 229, 1065
FokI	GGATG	2 cut(s)	50, 828
Fsp4HI	GCNGC	3 cut(s)	226, 229, 1065
FspBI	CTAG	1 cut(s)	281
FspI	TGCGCA	1 cut(s)	326
GlaI	GCGC	2 cut(s)	224, 326
GluI	GCNGC	3 cut(s)	226, 229, 1065
GsuI	CTGGAG	1 cut(s)	888
HaeII	RGCGCY	1 cut(s)	226
HaeIII	GGCC	1 cut(s)	1088
HapII	CCGG	1 cut(s)	219
HhaI	GCGC	2 cut(s)	225, 327
Hin1II	CATG	4 cut(s)	34, 656, 851, 945
Hin6I	GCGC	2 cut(s)	223, 325
HinP1I	GCGC	2 cut(s)	223, 325
HincII	GTYRAC	1 cut(s)	853
HindII	GTYRAC	1 cut(s)	853
HinfI	GANTC	5 cut(s)	110, 263, 499, 646, 829
HpaII	CCGG	1 cut(s)	219
HphI	GGTGA	2 cut(s)	203, 459
Hpy166II	GTNNAC	2 cut(s)	445, 853
Hpy188I	TCNGA	4 cut(s)	490, 733, 790, 828
Hpy188III	TCNNGA	6 cut(s)	296, 304, 389, 610, 905, 936
Hpy8I	GTNNAC	2 cut(s)	445, 853
Hpy99I	CGWCG	1 cut(s)	236
HpyAV	CCTTC	2 cut(s)	53, 848
HpyCH4III	ACNGT	6 cut(s)	55, 236, 322, 557, 1046, 1146
HpyCH4IV	ACGT	5 cut(s)	36, 309, 403, 962, 1006
HpyCH4V	TGCA	9 cut(s)	34, 353, 371, 477, 495, 539, 699, 1016, 1025
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1022
HpyF3I	CTNAG	4 cut(s)	431, 789, 821, 882
HpySE526I	ACGT	5 cut(s)	36, 309, 403, 962, 1006
Hsp92II	CATG	4 cut(s)	34, 656, 851, 945
HspAI	GCGC	2 cut(s)	223, 325
KpnI	GGTACC	1 cut(s)	677
Ksp22I	TGATCA	1 cut(s)	60
Kzo9I	GATC	3 cut(s)	60, 868, 1001
LmnI	GCTCC	2 cut(s)	362, 691
Lsp1109I	GCAGC	3 cut(s)	212, 215, 1076
LweI	GCATC	1 cut(s)	668
MabI	ACCWGGT	1 cut(s)	770
MaeI	CTAG	1 cut(s)	281
MaeII	ACGT	5 cut(s)	36, 309, 403, 962, 1006
MalI	GATC	3 cut(s)	62, 870, 1003
MboI	GATC	3 cut(s)	60, 868, 1001
MboII	GAAGA	3 cut(s)	172, 477, 986
MfeI	CAATTG	2 cut(s)	690, 890
MflI	RGATCY	1 cut(s)	868
MluCI	AATT	8 cut(s)	76, 346, 435, 585, 690, 890, 967, 1101
MmeI	TCCRAC	1 cut(s)	340
MseI	TTAA	6 cut(s)	242, 591, 755, 776, 810, 1094
MslI	CAYNNNNRTG	3 cut(s)	472, 846, 1144
MspCI	CTTAAG	1 cut(s)	809
MspI	CCGG	1 cut(s)	219
MspR9I	CCNGG	4 cut(s)	220, 678, 772, 999
MunI	CAATTG	2 cut(s)	690, 890
Mva1269I	GAATGC	2 cut(s)	465, 699
MvaI	CCWGG	3 cut(s)	678, 772, 999
MvnI	CGCG	1 cut(s)	74
MwoI	GCNNNNNNNGC	1 cut(s)	1022
NciI	CCSGG	1 cut(s)	220
NdeI	CATATG	1 cut(s)	598
NdeII	GATC	3 cut(s)	60, 868, 1001
NlaIII	CATG	4 cut(s)	34, 656, 851, 945
NlaIV	GGNNCC	4 cut(s)	165, 364, 531, 675
NsbI	TGCGCA	1 cut(s)	326
NspI	RCATGY	1 cut(s)	945
NspV	TTCGAA	1 cut(s)	832
OliI	CACNNNNGTG	1 cut(s)	1144
PaeI	GCATGC	1 cut(s)	945
PceI	AGGCCT	1 cut(s)	1088
PctI	GAATGC	2 cut(s)	465, 699
PfeI	GAWTC	5 cut(s)	110, 263, 499, 646, 829
Pfl23II	CGTACG	1 cut(s)	69
PfoI	TCCNGGA	1 cut(s)	997
PkrI	GCNGC	3 cut(s)	227, 230, 1066
PmaCI	CACGTG	1 cut(s)	1007
PmlI	CACGTG	1 cut(s)	1007
Ppu21I	YACGTR	1 cut(s)	1007
Psp6I	CCWGG	3 cut(s)	676, 770, 997
PspCI	CACGTG	1 cut(s)	1007
PspGI	CCWGG	3 cut(s)	676, 770, 997
PspLI	CGTACG	1 cut(s)	69
PspN4I	GGNNCC	4 cut(s)	165, 364, 531, 675
PspPI	GGNCC	2 cut(s)	164, 530
PstI	CTGCAG	1 cut(s)	497
PsuI	RGATCY	1 cut(s)	868
RsaI	GTAC	6 cut(s)	71, 170, 285, 675, 965, 1126
RsaNI	GTAC	6 cut(s)	70, 169, 284, 674, 964, 1125
RseI	CAYNNNNRTG	3 cut(s)	472, 846, 1144
SaqAI	TTAA	6 cut(s)	242, 591, 755, 776, 810, 1094
SatI	GCNGC	3 cut(s)	226, 229, 1065
Sau3AI	GATC	3 cut(s)	60, 868, 1001
Sau96I	GGNCC	2 cut(s)	164, 530
ScrFI	CCNGG	4 cut(s)	220, 678, 772, 999
SexAI	ACCWGGT	1 cut(s)	770
SfaNI	GCATC	1 cut(s)	668
SfcI	CTRYAG	1 cut(s)	493
SfuI	TTCGAA	1 cut(s)	832
SinI	GGWCC	2 cut(s)	164, 530
SmiMI	CAYNNNNRTG	3 cut(s)	472, 846, 1144
SmlI	CTYRAG	1 cut(s)	809
SmoI	CTYRAG	1 cut(s)	809
SpeI	ACTAGT	1 cut(s)	280
SphI	GCATGC	1 cut(s)	945
Sse9I	AATT	8 cut(s)	76, 346, 435, 585, 690, 890, 967, 1101
SseBI	AGGCCT	1 cut(s)	1088
SspMI	CTAG	1 cut(s)	281
StuI	AGGCCT	1 cut(s)	1088
StyD4I	CCNGG	4 cut(s)	218, 676, 770, 997
StyI	CCWWGG	1 cut(s)	1083
TaaI	ACNGT	6 cut(s)	55, 236, 322, 557, 1046, 1146
TaiI	ACGT	5 cut(s)	39, 312, 406, 965, 1009
TaqI	TCGA	2 cut(s)	662, 832
TaqII	GACCGA	1 cut(s)	547
TasI	AATT	8 cut(s)	76, 346, 435, 585, 690, 890, 967, 1101
TfiI	GAWTC	5 cut(s)	110, 263, 499, 646, 829
Tru1I	TTAA	6 cut(s)	242, 591, 755, 776, 810, 1094
Tru9I	TTAA	6 cut(s)	242, 591, 755, 776, 810, 1094
TscAI	CASTG	3 cut(s)	373, 385, 1079
TseI	GCWGC	3 cut(s)	225, 228, 1064
TspDTI	ATGAA	3 cut(s)	197, 330, 830
TspGWI	ACGGA	3 cut(s)	156, 549, 594
TspRI	CASTG	3 cut(s)	373, 385, 1079
Vha464I	CTTAAG	1 cut(s)	809
VpaK11BI	GGWCC	2 cut(s)	164, 530
XagI	CCTNNNNNAGG	1 cut(s)	335
XapI	RAATTY	3 cut(s)	346, 435, 1101
XceI	RCATGY	1 cut(s)	945
XspI	CTAG	1 cut(s)	281

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.