AT1G28670

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001486
Physical Location & Seq
Reverse (-)
10074359 .. 10076457
2099 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G28670.1

Sequence Viewer

Length: 1155 bp
ATGGCTTCTTCACTGAAGAAGCTTATCTCAAGCTTTCTACTTGTCTTATACTCCACCACCATCATTGTTGCTTCATCAGAATCTCGATGTAGGCGTTTTAAATCGATCATCAGCTTTGGTGATTCCATCGCAGACACCGGAAATTATCTCCATCTCTCTGATGTCAATCACCTTCCTCAATCCGCCTTTCTTCCTTATGGCGAAAGCTTCTTCCATCCTCCCTCCGGTCGTGCCTCTAATGGCCGTCTCATCATCGACTTCATTGCCGAATTCTTGGGACTACCATACGTACCGCCTTACTTTGGATCCCAAAACGTTAGCTTCGAACAAGGGATCAATTTTGCGGTGTATGGAGCAACCGCATTGGACCGAGCGTTTCTTTTGGGAAAAGGAATTGAATCTGATTTCACCAATGTTAGTTTAAGTGTTCAGCTTGACACCTTCAAGCAGATTTTGCCTAACTTATGCGCCTCGTCTACTCGTGATTGCAAAGAGATGCTTGGAGACTCGCTGATACTCATGGGAGAGATTGGAGGAAACGACTATAATTACCCATTTTTTGAAGGAAAAAGTATCAATGAAATCAAAGAGCTTGTTCCTCTAATCGTCAAAGCTATTTCTTCTGCTATTGTGGATTTGATTGATTTAGGGGGCAAAACATTTTTGGTACCCGGAGGCTTCCCAACAGGATGTTCTGCGGCGTATCTTACTCTATTTCAGACCGTGGCAGAAAAAGACCAGGACCCTTTAACAGGTTGTTACCCATTGCTCAACGAATTTGGCGAGCACCACAACGAACAGCTAAAGACAGAACTCAAGCGACTCCAAAAATTCTATCCTCATGTCAACATCATTTACGCTGACTACCACAACTCCTTATACCGGTTTTATCAAGAACCAGCTAAATACGGGTTTAAGAACAAACCTTTAGCTGCTTGCTGTGGAGTCGGAGGTAAATACAACTTCACTATTGGTAAGGAGTGTGGATACGAAGGAGTTAACTATTGTCAAAATCCTTCTGAGTATGTGAACTGGGATGGTTATCATTTAACCGAAGCCGCCTACCAGAAGATGACTGAGGGTATACTCAACGGTCCCTATGCAACTCCTGCTTTCGACTGGTCCTGCCTTGGCTCTGGTACAGTGGATACATAA

Protein Analysis

384

Amino Acids

42.43

Weight (kDa)

5.32

Isoelectric Point (pI)

36.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 36 - 361 6.3e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 667
AccB1I GGYRCC 1 cut(s) 667
AccI GTMKAC 2 cut(s) 476, 1084
AciI CCGC 6 cut(s) 183, 293, 344, 360, 698, 1059
AclI AACGTT 1 cut(s) 315
AclWI GGATC 3 cut(s) 300, 313, 341
AcoI YGGCCR 1 cut(s) 241
AcsI RAATTY 3 cut(s) 269, 776, 830
AcuI CTGAAG 1 cut(s) 35
AfaI GTAC 3 cut(s) 291, 669, 1141
AfiI CCNNNNNNNGG 4 cut(s) 224, 302, 752, 882
AgeI ACCGGT 1 cut(s) 882
AgsI TTSAA 3 cut(s) 398, 445, 563
AjnI CCWGG 1 cut(s) 738
AjuI GAANNNNNNNTTGG 2 cut(s) 676, 708
Alw21I GWGCWC 1 cut(s) 789
Alw26I GTCTC 2 cut(s) 251, 498
AlwI GGATC 3 cut(s) 300, 313, 341
AoxI GGCC 1 cut(s) 241
ApeKI GCWGC 1 cut(s) 932
ApoI RAATTY 3 cut(s) 269, 776, 830
AsiGI ACCGGT 1 cut(s) 882
Asp718I GGTACC 1 cut(s) 667
AspLEI GCGC 1 cut(s) 470
AspS9I GGNCC 4 cut(s) 367, 742, 1094, 1122
AsuC2I CCSGG 1 cut(s) 672
AsuHPI GGTGA 3 cut(s) 131, 161, 400
AsuII TTCGAA 1 cut(s) 324
AvaII GGWCC 4 cut(s) 367, 742, 1094, 1122
BamHI GGATCC 1 cut(s) 305
BanI GGYRCC 1 cut(s) 667
BauI CACGAG 1 cut(s) 480
Bbv12I GWGCWC 1 cut(s) 789
BbvI GCAGC 1 cut(s) 919
BccI CCATC 5 cut(s) 68, 134, 159, 222, 1031
BceAI ACGGC 1 cut(s) 228
BcgI CGANNNNNNTGC 2 cut(s) 245, 279
BciT130I CCWGG 1 cut(s) 740
BciVI GTATCC 2 cut(s) 980, 1141
BcnI CCSGG 1 cut(s) 672
BcoDI GTCTC 2 cut(s) 251, 498
BfuI GTATCC 2 cut(s) 980, 1141
BisI GCNGC 3 cut(s) 699, 933, 1059
BlsI GCNGC 3 cut(s) 700, 934, 1060
Bme1390I CCNGG 2 cut(s) 672, 740
Bme18I GGWCC 4 cut(s) 367, 742, 1094, 1122
BmgT120I GGNCC 4 cut(s) 367, 742, 1094, 1122
BmiI GGNNCC 4 cut(s) 307, 669, 744, 1096
BmrFI CCNGG 2 cut(s) 672, 740
BmrI ACTGGG 1 cut(s) 1042
BmsI GCATC 1 cut(s) 486
BmuI ACTGGG 1 cut(s) 1042
Bpu14I TTCGAA 1 cut(s) 324
BpuEI CTTGAG 2 cut(s) 13, 800
BpuMI CCSGG 1 cut(s) 672
Bsa29I ATCGAT 1 cut(s) 104
BsaAI YACGTR 1 cut(s) 289
BsaBI GATNNNNATC 2 cut(s) 165, 1041
BsaJI CCNNGG 2 cut(s) 723, 1129
BsaWI WCCGGW 3 cut(s) 137, 224, 882
BsaXI ACNNNNNCTCC 2 cut(s) 857, 887
Bsc4I CCNNNNNNNGG 4 cut(s) 224, 302, 752, 882
Bse118I RCCGGY 1 cut(s) 882
Bse1I ACTGG 2 cut(s) 1037, 1124
Bse3DI GCAATG 2 cut(s) 261, 764
Bse8I GATNNNNATC 2 cut(s) 165, 1041
BseBI CCWGG 1 cut(s) 740
BseCI ATCGAT 1 cut(s) 104
BseDI CCNNGG 2 cut(s) 723, 1129
BseGI GGATG 3 cut(s) 214, 695, 1042
BseJI GATNNNNATC 2 cut(s) 165, 1041
BseLI CCNNNNNNNGG 4 cut(s) 224, 302, 752, 882
BseMI GCAATG 2 cut(s) 261, 764
BseMII CTCAG 2 cut(s) 1011, 1068
BseNI ACTGG 2 cut(s) 1037, 1124
BseXI GCAGC 1 cut(s) 919
Bsh1285I CGRYCG 1 cut(s) 229
BshFI GGCC 1 cut(s) 243
BshNI GGYRCC 1 cut(s) 667
BshTI ACCGGT 1 cut(s) 882
BshVI ATCGAT 1 cut(s) 104
BsiEI CGRYCG 1 cut(s) 229
BsiHKAI GWGCWC 1 cut(s) 789
BsiSI CCGG 4 cut(s) 138, 225, 672, 883
BslFI GGGAC 2 cut(s) 291, 1080
BslI CCNNNNNNNGG 4 cut(s) 224, 302, 752, 882
BsmAI GTCTC 2 cut(s) 251, 498
BsmBI CGTCTC 1 cut(s) 251
BsmFI GGGAC 2 cut(s) 291, 1080
BsnI GGCC 1 cut(s) 243
Bsp119I TTCGAA 1 cut(s) 324
Bsp1286I GDGCHC 1 cut(s) 789
Bsp143I GATC 3 cut(s) 105, 305, 333
BspACI CCGC 6 cut(s) 183, 293, 344, 360, 698, 1059
BspANI GGCC 1 cut(s) 243
BspCNI CTCAG 2 cut(s) 1012, 1069
BspDI ATCGAT 1 cut(s) 104
BspLI GGNNCC 4 cut(s) 307, 669, 744, 1096
BspPI GGATC 3 cut(s) 300, 313, 341
BspT104I TTCGAA 1 cut(s) 324
BspT107I GGYRCC 1 cut(s) 667
BsrDI GCAATG 2 cut(s) 261, 764
BsrFI RCCGGY 1 cut(s) 882
BsrI ACTGG 2 cut(s) 1037, 1124
BssAI RCCGGY 1 cut(s) 882
BssECI CCNNGG 2 cut(s) 723, 1129
BssMI GATC 3 cut(s) 105, 305, 333
BssNAI GTATAC 1 cut(s) 1085
BssSI CACGAG 1 cut(s) 480
BssT1I CCWWGG 1 cut(s) 1129
Bst1107I GTATAC 1 cut(s) 1085
Bst2BI CACGAG 1 cut(s) 480
Bst2UI CCWGG 1 cut(s) 740
Bst4CI ACNGT 3 cut(s) 724, 1094, 1144
BstAPI GCANNNNNTGC 2 cut(s) 454, 1109
BstBAI YACGTR 1 cut(s) 289
BstBI TTCGAA 1 cut(s) 324
BstC8I GCNNGC 2 cut(s) 785, 937
BstDEI CTNAG 2 cut(s) 1020, 1077
BstDSI CCRYGG 1 cut(s) 723
BstENI CCTNNNNNAGG 1 cut(s) 750
BstF5I GGATG 3 cut(s) 214, 695, 1042
BstHHI GCGC 1 cut(s) 470
BstKTI GATC 3 cut(s) 108, 308, 336
BstMAI GTCTC 2 cut(s) 251, 498
BstMBI GATC 3 cut(s) 105, 305, 333
BstMCI CGRYCG 1 cut(s) 229
BstMWI GCNNNNNNNGC 2 cut(s) 454, 1109
BstNI CCWGG 1 cut(s) 740
BstSCI CCNGG 2 cut(s) 670, 738
BstSNI TACGTA 1 cut(s) 289
BstV1I GCAGC 1 cut(s) 919
BstX2I RGATCY 1 cut(s) 305
BstYI RGATCY 1 cut(s) 305
BstZ17I GTATAC 1 cut(s) 1085
Bsu15I ATCGAT 1 cut(s) 104
BsuI GTATCC 2 cut(s) 980, 1141
BsuRI GGCC 1 cut(s) 243
BsuTUI ATCGAT 1 cut(s) 104
BtgI CCRYGG 1 cut(s) 723
BtgZI GCGATG 1 cut(s) 112
BtsCI GGATG 3 cut(s) 214, 695, 1042
BtsIMutI CAGTG 2 cut(s) 11, 1149
Cac8I GCNNGC 2 cut(s) 785, 937
CfoI GCGC 1 cut(s) 470
Cfr10I RCCGGY 1 cut(s) 882
Cfr13I GGNCC 4 cut(s) 367, 742, 1094, 1122
ClaI ATCGAT 1 cut(s) 104
Csp6I GTAC 3 cut(s) 290, 668, 1140
CspAI ACCGGT 1 cut(s) 882
CspCI CAANNNNNGTGG 2 cut(s) 46, 81
CviAII CATG 2 cut(s) 520, 842
CviQI GTAC 3 cut(s) 290, 668, 1140
DdeI CTNAG 2 cut(s) 1020, 1077
DpnI GATC 3 cut(s) 107, 307, 335
DpnII GATC 3 cut(s) 105, 305, 333
DraI TTTAAA 1 cut(s) 100
EaeI YGGCCR 1 cut(s) 241
EciI GGCGGA 1 cut(s) 172
Eco105I TACGTA 1 cut(s) 289
Eco130I CCWWGG 1 cut(s) 1129
Eco47I GGWCC 4 cut(s) 367, 742, 1094, 1122
Eco57I CTGAAG 1 cut(s) 35
EcoNI CCTNNNNNAGG 1 cut(s) 750
EcoO109I RGGNCCY 1 cut(s) 742
EcoRI GAATTC 1 cut(s) 269
EcoRII CCWGG 1 cut(s) 738
EcoT14I CCWWGG 1 cut(s) 1129
ErhI CCWWGG 1 cut(s) 1129
Esp3I CGTCTC 1 cut(s) 251
FaeI CATG 2 cut(s) 523, 845
FalI AAGNNNNNCTT 2 cut(s) 483, 515
FaqI GGGAC 2 cut(s) 291, 1080
FatI CATG 2 cut(s) 519, 841
FblI GTMKAC 2 cut(s) 476, 1084
Fnu4HI GCNGC 3 cut(s) 699, 933, 1059
FokI GGATG 3 cut(s) 201, 702, 1049
Fsp4HI GCNGC 3 cut(s) 699, 933, 1059
GlaI GCGC 1 cut(s) 469
GluI GCNGC 3 cut(s) 699, 933, 1059
HaeIII GGCC 1 cut(s) 243
HapII CCGG 4 cut(s) 138, 225, 672, 883
HhaI GCGC 1 cut(s) 470
Hin1II CATG 2 cut(s) 523, 845
Hin6I GCGC 1 cut(s) 468
HinP1I GCGC 1 cut(s) 468
HincII GTYRAC 2 cut(s) 847, 1000
HindII GTYRAC 2 cut(s) 847, 1000
HindIII AAGCTT 3 cut(s) 20, 31, 205
HinfI GANTC 6 cut(s) 80, 122, 398, 506, 822, 945
HpaI GTTAAC 1 cut(s) 1000
HpaII CCGG 4 cut(s) 138, 225, 672, 883
HphI GGTGA 3 cut(s) 131, 161, 400
Hpy166II GTNNAC 5 cut(s) 477, 847, 1000, 1030, 1085
Hpy188I TCNGA 6 cut(s) 79, 160, 403, 720, 950, 1021
Hpy188III TCNNGA 3 cut(s) 84, 482, 893
Hpy8I GTNNAC 5 cut(s) 477, 847, 1000, 1030, 1085
HpyAV CCTTC 5 cut(s) 182, 451, 557, 986, 1026
HpyCH4III ACNGT 3 cut(s) 724, 1094, 1144
HpyCH4IV ACGT 2 cut(s) 288, 315
HpyCH4V TGCA 2 cut(s) 489, 1103
HpyF10VI GCNNNNNNNGC 2 cut(s) 454, 1109
HpyF3I CTNAG 2 cut(s) 1020, 1077
HpySE526I ACGT 2 cut(s) 288, 315
Hsp92II CATG 2 cut(s) 523, 845
HspAI GCGC 1 cut(s) 468
KpnI GGTACC 1 cut(s) 671
KspAI GTTAAC 1 cut(s) 1000
Kzo9I GATC 3 cut(s) 105, 305, 333
LmnI GCTCC 1 cut(s) 353
Lsp1109I GCAGC 1 cut(s) 919
LweI GCATC 1 cut(s) 486
MaeII ACGT 2 cut(s) 288, 315
MaeIII GTNAC 1 cut(s) 758
MalI GATC 3 cut(s) 107, 307, 335
MboI GATC 3 cut(s) 105, 305, 333
MboII GAAGA 5 cut(s) 28, 182, 202, 612, 1081
MflI RGATCY 1 cut(s) 305
MhlI GDGCHC 1 cut(s) 789
MluCI AATT 7 cut(s) 142, 269, 337, 393, 547, 776, 830
MlyI GAGTC 3 cut(s) 500, 816, 954
MmeI TCCRAC 1 cut(s) 928
MseI TTAA 6 cut(s) 99, 422, 749, 915, 999, 1049
MspI CCGG 4 cut(s) 138, 225, 672, 883
MspR9I CCNGG 2 cut(s) 672, 740
MvaI CCWGG 1 cut(s) 740
MwoI GCNNNNNNNGC 2 cut(s) 454, 1109
NciI CCSGG 1 cut(s) 672
NdeII GATC 3 cut(s) 105, 305, 333
NlaIII CATG 2 cut(s) 523, 845
NlaIV GGNNCC 4 cut(s) 307, 669, 744, 1096
NspV TTCGAA 1 cut(s) 324
PcsI WCGNNNNNNNCGW 3 cut(s) 91, 321, 780
PfeI GAWTC 3 cut(s) 80, 122, 398
PinAI ACCGGT 1 cut(s) 882
PkrI GCNGC 3 cut(s) 700, 934, 1060
PleI GAGTC 3 cut(s) 500, 816, 953
PpsI GAGTC 3 cut(s) 500, 816, 953
Ppu21I YACGTR 1 cut(s) 289
PpuMI RGGWCCY 1 cut(s) 742
Psp1406I AACGTT 1 cut(s) 315
Psp5II RGGWCCY 1 cut(s) 742
Psp6I CCWGG 1 cut(s) 738
PspGI CCWGG 1 cut(s) 738
PspN4I GGNNCC 4 cut(s) 307, 669, 744, 1096
PspPI GGNCC 4 cut(s) 367, 742, 1094, 1122
PspPPI RGGWCCY 1 cut(s) 742
PsuI RGATCY 1 cut(s) 305
RsaI GTAC 3 cut(s) 291, 669, 1141
RsaNI GTAC 3 cut(s) 290, 668, 1140
SaqAI TTAA 6 cut(s) 99, 422, 749, 915, 999, 1049
SatI GCNGC 3 cut(s) 699, 933, 1059
Sau3AI GATC 3 cut(s) 105, 305, 333
Sau96I GGNCC 4 cut(s) 367, 742, 1094, 1122
SchI GAGTC 3 cut(s) 500, 816, 954
ScrFI CCNGG 2 cut(s) 672, 740
SduI GDGCHC 1 cut(s) 789
SfaNI GCATC 1 cut(s) 486
SfuI TTCGAA 1 cut(s) 324
SinI GGWCC 4 cut(s) 367, 742, 1094, 1122
SmlI CTYRAG 2 cut(s) 28, 815
SmoI CTYRAG 2 cut(s) 28, 815
SnaBI TACGTA 1 cut(s) 289
Sse9I AATT 7 cut(s) 142, 269, 337, 393, 547, 776, 830
SsiI CCGC 6 cut(s) 183, 293, 344, 360, 698, 1059
StyD4I CCNGG 2 cut(s) 670, 738
StyI CCWWGG 1 cut(s) 1129
TaaI ACNGT 3 cut(s) 724, 1094, 1144
TaiI ACGT 2 cut(s) 291, 318
TaqI TCGA 5 cut(s) 85, 104, 255, 324, 1116
TaqII GACCGA 1 cut(s) 384
TasI AATT 7 cut(s) 142, 269, 337, 393, 547, 776, 830
TauI GCSGC 2 cut(s) 701, 1061
TfiI GAWTC 3 cut(s) 80, 122, 398
Tru1I TTAA 6 cut(s) 99, 422, 749, 915, 999, 1049
Tru9I TTAA 6 cut(s) 99, 422, 749, 915, 999, 1049
TscAI CASTG 2 cut(s) 18, 1149
TseI GCWGC 1 cut(s) 932
TspDTI ATGAA 3 cut(s) 63, 250, 594
TspRI CASTG 2 cut(s) 18, 1149
VpaK11BI GGWCC 4 cut(s) 367, 742, 1094, 1122
XagI CCTNNNNNAGG 1 cut(s) 750
XapI RAATTY 3 cut(s) 269, 776, 830
XmiI GTMKAC 2 cut(s) 476, 1084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.