CRAL/TRIO, N-terminal domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 2 cut(s) | 698, 883 |
| AccI | GTMKAC | 1 cut(s) | 1278 |
| AccII | CGCG | 1 cut(s) | 828 |
| AccIII | TCCGGA | 1 cut(s) | 1081 |
| AciI | CCGC | 3 cut(s) | 826, 1200, 1394 |
| AclI | AACGTT | 1 cut(s) | 1113 |
| AclWI | GGATC | 4 cut(s) | 760, 798, 985, 1339 |
| AcsI | RAATTY | 2 cut(s) | 1085, 1289 |
| AcuI | CTGAAG | 6 cut(s) | 30, 348, 567, 743, 1332, 1389 |
| AdeI | CACNNNGTG | 1 cut(s) | 218 |
| AfaI | GTAC | 5 cut(s) | 1229, 1259, 1375, 1589, 1595 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 641, 738, 775, 862 |
| AgsI | TTSAA | 7 cut(s) | 116, 163, 193, 377, 673, 1270, 1519 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 829 |
| AjnI | CCWGG | 3 cut(s) | 964, 1012, 1327 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1018, 1050 |
| Alw21I | GWGCWC | 2 cut(s) | 290, 1248 |
| AlwI | GGATC | 4 cut(s) | 760, 798, 985, 1339 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1361 |
| Aor13HI | TCCGGA | 1 cut(s) | 1081 |
| ApeKI | GCWGC | 2 cut(s) | 359, 803 |
| ApoI | RAATTY | 2 cut(s) | 1085, 1289 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1514 |
| AspS9I | GGNCC | 2 cut(s) | 823, 1499 |
| AsuHPI | GGTGA | 1 cut(s) | 1486 |
| AvaII | GGWCC | 2 cut(s) | 823, 1499 |
| AxyI | CCTNAGG | 2 cut(s) | 72, 135 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1365, 1398 |
| BanII | GRGCYC | 2 cut(s) | 290, 1248 |
| Bbv12I | GWGCWC | 2 cut(s) | 290, 1248 |
| BbvCI | CCTCAGC | 1 cut(s) | 333 |
| BbvI | GCAGC | 2 cut(s) | 371, 815 |
| BccI | CCATC | 5 cut(s) | 909, 925, 1352, 1398, 1477 |
| BciT130I | CCWGG | 3 cut(s) | 966, 1014, 1329 |
| BfmI | CTRYAG | 1 cut(s) | 801 |
| BisI | GCNGC | 2 cut(s) | 360, 804 |
| BlsI | GCNGC | 2 cut(s) | 361, 805 |
| Bme1390I | CCNGG | 3 cut(s) | 966, 1014, 1329 |
| Bme18I | GGWCC | 2 cut(s) | 823, 1499 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 829 |
| BmgT120I | GGNCC | 2 cut(s) | 823, 1499 |
| BmrFI | CCNGG | 3 cut(s) | 966, 1014, 1329 |
| BmrI | ACTGGG | 2 cut(s) | 833, 1244 |
| BmsI | GCATC | 1 cut(s) | 708 |
| BmuI | ACTGGG | 2 cut(s) | 833, 1244 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1131, 1163 |
| BpmI | CTGGAG | 2 cut(s) | 971, 1035 |
| Bpu10I | CCTNAGC | 2 cut(s) | 333, 1203 |
| BpuEI | CTTGAG | 5 cut(s) | 209, 257, 700, 931, 1208 |
| BsaJI | CCNNGG | 4 cut(s) | 465, 510, 531, 769 |
| BsaWI | WCCGGW | 1 cut(s) | 1081 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 641, 738, 775, 862 |
| Bse1I | ACTGG | 2 cut(s) | 839, 1250 |
| Bse21I | CCTNAGG | 2 cut(s) | 72, 135 |
| Bse3DI | GCAATG | 1 cut(s) | 626 |
| BseAI | TCCGGA | 1 cut(s) | 1081 |
| BseBI | CCWGG | 3 cut(s) | 966, 1014, 1329 |
| BseDI | CCNNGG | 4 cut(s) | 465, 510, 531, 769 |
| BseGI | GGATG | 4 cut(s) | 541, 586, 664, 1409 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 641, 738, 775, 862 |
| BseMI | GCAATG | 1 cut(s) | 626 |
| BseMII | CTCAG | 9 cut(s) | 63, 126, 192, 206, 324, 384, 417, 552, 1353 |
| BseNI | ACTGG | 2 cut(s) | 839, 1250 |
| BseRI | GAGGAG | 6 cut(s) | 38, 89, 380, 467, 791, 878 |
| BseXI | GCAGC | 2 cut(s) | 371, 815 |
| BseYI | CCCAGC | 1 cut(s) | 1354 |
| Bsh1236I | CGCG | 1 cut(s) | 828 |
| BsiHKAI | GWGCWC | 2 cut(s) | 290, 1248 |
| BsiSI | CCGG | 1 cut(s) | 1082 |
| BslFI | GGGAC | 3 cut(s) | 817, 1182, 1400 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 641, 738, 775, 862 |
| BsmFI | GGGAC | 3 cut(s) | 817, 1182, 1400 |
| Bsp1286I | GDGCHC | 2 cut(s) | 290, 1248 |
| Bsp13I | TCCGGA | 1 cut(s) | 1081 |
| Bsp1407I | TGTACA | 1 cut(s) | 1587 |
| Bsp143I | GATC | 6 cut(s) | 220, 765, 790, 990, 997, 1331 |
| BspACI | CCGC | 3 cut(s) | 826, 1200, 1394 |
| BspCNI | CTCAG | 9 cut(s) | 64, 127, 193, 207, 325, 385, 418, 553, 1354 |
| BspEI | TCCGGA | 1 cut(s) | 1081 |
| BspFNI | CGCG | 1 cut(s) | 828 |
| BspMAI | CTGCAG | 1 cut(s) | 805 |
| BspPI | GGATC | 4 cut(s) | 760, 798, 985, 1339 |
| BsrDI | GCAATG | 1 cut(s) | 626 |
| BsrGI | TGTACA | 1 cut(s) | 1587 |
| BsrI | ACTGG | 2 cut(s) | 839, 1250 |
| BssECI | CCNNGG | 4 cut(s) | 465, 510, 531, 769 |
| BssMI | GATC | 6 cut(s) | 220, 765, 790, 990, 997, 1331 |
| BssT1I | CCWWGG | 3 cut(s) | 465, 510, 769 |
| Bst2UI | CCWGG | 3 cut(s) | 966, 1014, 1329 |
| Bst6I | CTCTTC | 9 cut(s) | 96, 143, 162, 306, 375, 513, 987, 1224, 1419 |
| BstAUI | TGTACA | 1 cut(s) | 1587 |
| BstC8I | GCNNGC | 1 cut(s) | 131 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 773 |
| BstF5I | GGATG | 4 cut(s) | 541, 586, 664, 1409 |
| BstFNI | CGCG | 1 cut(s) | 828 |
| BstKTI | GATC | 6 cut(s) | 223, 768, 793, 993, 1000, 1334 |
| BstMBI | GATC | 6 cut(s) | 220, 765, 790, 990, 997, 1331 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 422, 803, 1139, 1199 |
| BstNI | CCWGG | 3 cut(s) | 966, 1014, 1329 |
| BstSCI | CCNGG | 3 cut(s) | 964, 1012, 1327 |
| BstSFI | CTRYAG | 1 cut(s) | 801 |
| BstUI | CGCG | 1 cut(s) | 828 |
| BstV1I | GCAGC | 2 cut(s) | 371, 815 |
| BstX2I | RGATCY | 2 cut(s) | 790, 990 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 881 |
| BstYI | RGATCY | 2 cut(s) | 790, 990 |
| Bsu36I | CCTNAGG | 2 cut(s) | 72, 135 |
| BtsCI | GGATG | 4 cut(s) | 541, 586, 664, 1409 |
| BtsI | GCAGTG | 1 cut(s) | 798 |
| BtsIMutI | CAGTG | 2 cut(s) | 212, 798 |
| Cac8I | GCNNGC | 1 cut(s) | 131 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1361 |
| Cfr13I | GGNCC | 2 cut(s) | 823, 1499 |
| Csp6I | GTAC | 5 cut(s) | 1228, 1258, 1374, 1588, 1594 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 824, 859 |
| CviAII | CATG | 2 cut(s) | 811, 1135 |
| CviQI | GTAC | 5 cut(s) | 1228, 1258, 1374, 1588, 1594 |
| DpnI | GATC | 6 cut(s) | 222, 767, 792, 992, 999, 1333 |
| DpnII | GATC | 6 cut(s) | 220, 765, 790, 990, 997, 1331 |
| DraI | TTTAAA | 1 cut(s) | 697 |
| DraIII | CACNNNGTG | 1 cut(s) | 218 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 698, 883 |
| DriI | GACNNNNNGTC | 1 cut(s) | 829 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 698, 883 |
| Eam1104I | CTCTTC | 9 cut(s) | 96, 143, 162, 306, 375, 513, 987, 1224, 1419 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 829 |
| EarI | CTCTTC | 9 cut(s) | 96, 143, 162, 306, 375, 513, 987, 1224, 1419 |
| Ecl136II | GAGCTC | 2 cut(s) | 288, 1246 |
| Eco130I | CCWWGG | 3 cut(s) | 465, 510, 769 |
| Eco24I | GRGCYC | 2 cut(s) | 290, 1248 |
| Eco32I | GATATC | 1 cut(s) | 607 |
| Eco47I | GGWCC | 2 cut(s) | 823, 1499 |
| Eco53kI | GAGCTC | 2 cut(s) | 288, 1246 |
| Eco57I | CTGAAG | 6 cut(s) | 30, 348, 567, 743, 1332, 1389 |
| Eco81I | CCTNAGG | 2 cut(s) | 72, 135 |
| EcoICRI | GAGCTC | 2 cut(s) | 288, 1246 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 773 |
| EcoRI | GAATTC | 2 cut(s) | 1085, 1289 |
| EcoRII | CCWGG | 3 cut(s) | 964, 1012, 1327 |
| EcoRV | GATATC | 1 cut(s) | 607 |
| EcoT14I | CCWWGG | 3 cut(s) | 465, 510, 769 |
| EcoT38I | GRGCYC | 2 cut(s) | 290, 1248 |
| ErhI | CCWWGG | 3 cut(s) | 465, 510, 769 |
| FaeI | CATG | 2 cut(s) | 814, 1138 |
| FaiI | YATR | 6 cut(s) | 296, 812, 1103, 1136, 1384, 1391 |
| FaqI | GGGAC | 3 cut(s) | 817, 1182, 1400 |
| FatI | CATG | 2 cut(s) | 810, 1134 |
| FauI | CCCGC | 1 cut(s) | 1207 |
| FblI | GTMKAC | 1 cut(s) | 1278 |
| Fnu4HI | GCNGC | 2 cut(s) | 360, 804 |
| FokI | GGATG | 4 cut(s) | 548, 593, 671, 1416 |
| FriOI | GRGCYC | 2 cut(s) | 290, 1248 |
| Fsp4HI | GCNGC | 2 cut(s) | 360, 804 |
| GluI | GCNGC | 2 cut(s) | 360, 804 |
| GsaI | CCCAGC | 1 cut(s) | 1358 |
| GsuI | CTGGAG | 2 cut(s) | 971, 1035 |
| HapII | CCGG | 1 cut(s) | 1082 |
| Hin1II | CATG | 2 cut(s) | 814, 1138 |
| HincII | GTYRAC | 3 cut(s) | 253, 703, 1555 |
| HindII | GTYRAC | 3 cut(s) | 253, 703, 1555 |
| HindIII | AAGCTT | 2 cut(s) | 233, 950 |
| HinfI | GANTC | 9 cut(s) | 38, 197, 590, 761, 830, 942, 1044, 1216, 1610 |
| HpaII | CCGG | 1 cut(s) | 1082 |
| HphI | GGTGA | 1 cut(s) | 1486 |
| Hpy166II | GTNNAC | 6 cut(s) | 253, 703, 823, 856, 1279, 1555 |
| Hpy188I | TCNGA | 8 cut(s) | 43, 202, 394, 889, 1043, 1211, 1312, 1339 |
| Hpy8I | GTNNAC | 6 cut(s) | 253, 703, 823, 856, 1279, 1555 |
| HpyCH4IV | ACGT | 1 cut(s) | 1113 |
| HpyCH4V | TGCA | 2 cut(s) | 803, 1069 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 422, 803, 1139, 1199 |
| HpySE526I | ACGT | 1 cut(s) | 1113 |
| Hsp92II | CATG | 2 cut(s) | 814, 1138 |
| Kpn2I | TCCGGA | 1 cut(s) | 1081 |
| Kzo9I | GATC | 6 cut(s) | 220, 765, 790, 990, 997, 1331 |
| LmnI | GCTCC | 8 cut(s) | 61, 644, 865, 1015, 1251, 1363, 1448, 1487 |
| Lsp1109I | GCAGC | 2 cut(s) | 371, 815 |
| LweI | GCATC | 1 cut(s) | 708 |
| MaeII | ACGT | 1 cut(s) | 1113 |
| MaeIII | GTNAC | 6 cut(s) | 403, 445, 475, 541, 845, 973 |
| MalI | GATC | 6 cut(s) | 222, 767, 792, 992, 999, 1333 |
| MboI | GATC | 6 cut(s) | 220, 765, 790, 990, 997, 1331 |
| MflI | RGATCY | 2 cut(s) | 790, 990 |
| MhlI | GDGCHC | 2 cut(s) | 290, 1248 |
| MluCI | AATT | 3 cut(s) | 1085, 1289, 1414 |
| MlyI | GAGTC | 4 cut(s) | 47, 599, 839, 1210 |
| MmeI | TCCRAC | 3 cut(s) | 867, 1021, 1320 |
| MroI | TCCGGA | 1 cut(s) | 1081 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1514 |
| MseI | TTAA | 8 cut(s) | 15, 87, 266, 696, 954, 960, 1160, 1323 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1381 |
| MspA1I | CMGCKG | 2 cut(s) | 928, 1238 |
| MspI | CCGG | 1 cut(s) | 1082 |
| MspR9I | CCNGG | 3 cut(s) | 966, 1014, 1329 |
| MvaI | CCWGG | 3 cut(s) | 966, 1014, 1329 |
| MvnI | CGCG | 1 cut(s) | 828 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 422, 803, 1139, 1199 |
| NdeII | GATC | 6 cut(s) | 220, 765, 790, 990, 997, 1331 |
| NlaIII | CATG | 2 cut(s) | 814, 1138 |
| NmuCI | GTSAC | 1 cut(s) | 475 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1514 |
| PfeI | GAWTC | 5 cut(s) | 197, 761, 942, 1044, 1610 |
| PflFI | GACNNNGTC | 1 cut(s) | 1559 |
| PfoI | TCCNGGA | 1 cut(s) | 1012 |
| PkrI | GCNGC | 2 cut(s) | 361, 805 |
| PleI | GAGTC | 4 cut(s) | 46, 598, 838, 1210 |
| PpsI | GAGTC | 4 cut(s) | 46, 598, 838, 1210 |
| Psp124BI | GAGCTC | 2 cut(s) | 290, 1248 |
| Psp1406I | AACGTT | 1 cut(s) | 1113 |
| Psp6I | CCWGG | 3 cut(s) | 964, 1012, 1327 |
| PspFI | CCCAGC | 1 cut(s) | 1354 |
| PspGI | CCWGG | 3 cut(s) | 964, 1012, 1327 |
| PspPI | GGNCC | 2 cut(s) | 823, 1499 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1250, 1282 |
| PstI | CTGCAG | 1 cut(s) | 805 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1361 |
| PsuI | RGATCY | 2 cut(s) | 790, 990 |
| PsyI | GACNNNGTC | 1 cut(s) | 1559 |
| PvuII | CAGCTG | 2 cut(s) | 928, 1238 |
| RsaI | GTAC | 5 cut(s) | 1229, 1259, 1375, 1589, 1595 |
| RsaNI | GTAC | 5 cut(s) | 1228, 1258, 1374, 1588, 1594 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1381 |
| SacI | GAGCTC | 2 cut(s) | 290, 1248 |
| SaqAI | TTAA | 8 cut(s) | 15, 87, 266, 696, 954, 960, 1160, 1323 |
| SatI | GCNGC | 2 cut(s) | 360, 804 |
| Sau3AI | GATC | 6 cut(s) | 220, 765, 790, 990, 997, 1331 |
| Sau96I | GGNCC | 2 cut(s) | 823, 1499 |
| SchI | GAGTC | 4 cut(s) | 47, 599, 839, 1210 |
| ScrFI | CCNGG | 3 cut(s) | 966, 1014, 1329 |
| SduI | GDGCHC | 2 cut(s) | 290, 1248 |
| SfaNI | GCATC | 1 cut(s) | 708 |
| SfcI | CTRYAG | 1 cut(s) | 801 |
| SinI | GGWCC | 2 cut(s) | 823, 1499 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1381 |
| SmlI | CTYRAG | 5 cut(s) | 224, 236, 679, 910, 1223 |
| SmoI | CTYRAG | 5 cut(s) | 224, 236, 679, 910, 1223 |
| Sse9I | AATT | 3 cut(s) | 1085, 1289, 1414 |
| SsiI | CCGC | 3 cut(s) | 826, 1200, 1394 |
| SstI | GAGCTC | 2 cut(s) | 290, 1248 |
| StyD4I | CCNGG | 3 cut(s) | 964, 1012, 1327 |
| StyI | CCWWGG | 3 cut(s) | 465, 510, 769 |
| TaiI | ACGT | 1 cut(s) | 1116 |
| TaqI | TCGA | 4 cut(s) | 609, 764, 1059, 1347 |
| TasI | AATT | 3 cut(s) | 1085, 1289, 1414 |
| TatI | WGTACW | 2 cut(s) | 1227, 1587 |
| TfiI | GAWTC | 5 cut(s) | 197, 761, 942, 1044, 1610 |
| Tru1I | TTAA | 8 cut(s) | 15, 87, 266, 696, 954, 960, 1160, 1323 |
| Tru9I | TTAA | 8 cut(s) | 15, 87, 266, 696, 954, 960, 1160, 1323 |
| TscAI | CASTG | 2 cut(s) | 219, 805 |
| TseFI | GTSAC | 1 cut(s) | 475 |
| TseI | GCWGC | 2 cut(s) | 359, 803 |
| Tsp45I | GTSAC | 1 cut(s) | 475 |
| TspDTI | ATGAA | 5 cut(s) | 311, 597, 827, 1096, 1509 |
| TspGWI | ACGGA | 1 cut(s) | 671 |
| TspRI | CASTG | 2 cut(s) | 219, 805 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1559 |
| VpaK11BI | GGWCC | 2 cut(s) | 823, 1499 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 773 |
| XapI | RAATTY | 2 cut(s) | 1085, 1289 |
| XmiI | GTMKAC | 1 cut(s) | 1278 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1514 |