RchiOBHm_Chr5g0024581

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001644
Physical Location & Seq
Reverse (-)
18671254 .. 18673310
2057 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30432

Sequence Viewer

Length: 1668 bp
ATGGGAACAGAACAAAAGTTAGAAGAGGCCCAGAAGGAGAAGGGTGTAGTTGAAGAGGAGGTGAAGGAGGAGGAGAAGAAGAAGATTGAAGAAGAACCAAGGGAGATTGTGGTGGATGATAAGTTGAAAGCAGAAACAGGAAAGGAGGTGGGTTTGGAAGAGCAAAAGGATAATGTTGAGGTTGGAAATGGAGGTGCTTCTGAATCTGGTAGAGTTGAGAAATGGTCATCATTAAGGGAAGAAAGGAACTCCCTTTCAGAGTTGAAAGAGCATGAGAAAAAGTCATTGGCGGAGCTGAGATCGAAGATTGAAATTGCGATACATGAAAACAAGTTGTTAAAGGAAAACAAGGATCAAGTAGGAGAGGAAAATGTTTCAAAAGAAAATGAGAAAGAGAAGGAACTTTCAAAGAAATTAAAAGAGATTGAGGAGAAGGAAGAGAGAAACATAGAGACTAAAGAGAAAGGAGAAGAAATTGCTGTGAATGGAATAAAGAAAGAGAAGGAAACATCCAACGAAGTAGAGGAGGTTGAAGCTGCAAAACCAAACAAGCAAGAAGATGAGCAAAAGGAGAAAACAGCTGAACAGAAAAAGAAGAAGGTTGAGTTCAACACCAATAAGGATATTGCAATTTGGGGAGTACCCTTATTGCCTAGTAAAGAAGATAATGCCATTGATGTCATATTATTGAAATTCTTGAGGGCCAAGGAGTTTGAGGTAAACGATGCTTTTGAGATGTTAAGGAATACCCTCAAATGGAGGAAGGAGAACAACATCGATTCGATATTGGACGAAAACTTTGGTGATGACCTTAGTTCCATAAGTAGCATTGGTGGTGTTGGTCGTGAGGGACACCCGGTTTGTTACAGCAACTTTAAGCTGCTTGGGAATGAAGAATTATACAACAAGGTACTGGGAACTGAAGAGAAGCATAACATGTTTATAAGATCCAGAGTTCAACTAATGGAAAGAGCAATTCAGAAGCTCGACTTTAAGCCTGGTGGTGTGTCCTCAATATTCATGATCAATGATCTCAATCAAATGCCTGGTCCATCTAAGAAGGAGCTTCGAAATGCTATAAAGCAGGTGTTTGCTCTTCATCAGGACAATTATCCTGAACTCGTTGCAAGAAATATCTTCCTCAATGTTCCTTTTTGGTACTATGCCTTCAGTGCTAGCGCTCTGCTATCACCTTTTTTTACCCCAAGAACCAATGGCAAATTCATCTTCGCTCGGCCTTCCAGAGTCAATGACGTCTTGCTCAAGTACATAGCTTTAGAGGAACTACCAGTTTATTATGGTGGCCTCAGAAAGGAAAATGATCCCGATTTCTCTAGCGAAGATGTTGTCTCAGAGATCTTTATCAATCAATCATCATCAGAAAGTATACATATACCAGCACCAGAGGCAGGAATGACTTTGATGTGGGAAGTTCTAGTGTCGGGTTGGGAAGTGAACTACAAGGAAGAATTTATCCCAACAGAGGAGGGTTCATATGGCATTATTGTACAAAGGAGCAGGAGGATCGGCGCGCAGGAGGGATCAATCCGAAACTCTTTCACGACTAAGGAGCCCGGAATGATTGTGTTAACAATCGAAAATGGAGCAATAATCAAGAAGAAGCGAATTTGTTACCGATACAAGATCAAAAATGGGAGTTCCTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

555

Amino Acids

63.49

Weight (kDa)

5.25

Isoelectric Point (pI)

50.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_N PF03765 195 - 249 5e-08 CRAL/TRIO, N-terminal domain
CRAL_TRIO PF00650 276 - 435 4.7e-19 CRAL/TRIO domain
GOLD_PATL1_C PF25099 457 - 540 4.9e-30 Patellin-1-like, C-terminal GOLD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 944
AarI CACCTGC 1 cut(s) 1075
AatII GACGTC 1 cut(s) 1257
Acc36I ACCTGC 1 cut(s) 1075
AccI GTMKAC 1 cut(s) 1387
AccII CGCG 1 cut(s) 1532
AciI CCGC 1 cut(s) 290
AclWI GGATC 5 cut(s) 360, 942, 1316, 1532, 1549
AcsI RAATTY 4 cut(s) 692, 1220, 1469, 1626
AcuI CTGAAG 2 cut(s) 942, 1153
AcyI GRCGYC 1 cut(s) 1254
AfaI GTAC 5 cut(s) 642, 912, 1160, 1268, 1509
AfeI AGCGCT 1 cut(s) 1180
AfiI CCNNNNNNNGG 4 cut(s) 756, 1312, 1409, 1483
AflIII ACRYGT 1 cut(s) 936
AjnI CCWGG 2 cut(s) 997, 1045
AjuI GAANNNNNNNTTGG 4 cut(s) 269, 301, 1205, 1237
AluBI AGCT 7 cut(s) 295, 536, 581, 880, 985, 1066, 1274
AluI AGCT 7 cut(s) 295, 536, 581, 880, 985, 1066, 1274
Alw26I GTCTC 2 cut(s) 446, 1354
AlwI GGATC 5 cut(s) 360, 942, 1316, 1532, 1549
Aor51HI AGCGCT 1 cut(s) 1180
AoxI GGCC 4 cut(s) 27, 702, 1235, 1303
ApeKI GCWGC 2 cut(s) 536, 880
ApoI RAATTY 4 cut(s) 692, 1220, 1469, 1626
ArsI GACNNNNNNTTYG 8 cut(s) 782, 814, 843, 875, 1033, 1065, 1332, 1364
AspLEI GCGC 3 cut(s) 1181, 1532, 1534
AspS9I GGNCC 3 cut(s) 28, 702, 1049
AsuC2I CCSGG 2 cut(s) 857, 1575
AsuHPI GGTGA 3 cut(s) 73, 815, 1182
AsuII TTCGAA 1 cut(s) 1069
AsuNHI GCTAGC 1 cut(s) 1175
AvaII GGWCC 1 cut(s) 1049
BanII GRGCYC 1 cut(s) 1575
BarI GAAGNNNNNNTAC 2 cut(s) 1151, 1183
BauI CACGAG 1 cut(s) 1663
BbvI GCAGC 2 cut(s) 523, 867
BccI CCATC 1 cut(s) 1060
BcgI CGANNNNNNTGC 2 cut(s) 1507, 1541
BciT130I CCWGG 2 cut(s) 999, 1047
BclI TGATCA 1 cut(s) 1023
BcnI CCSGG 2 cut(s) 857, 1575
BcoDI GTCTC 2 cut(s) 446, 1354
BfaI CTAG 4 cut(s) 654, 1176, 1335, 1436
BfoI RGCGCY 1 cut(s) 1182
BfuAI ACCTGC 1 cut(s) 1075
BglII AGATCT 1 cut(s) 1356
BisI GCNGC 2 cut(s) 537, 881
BlsI GCNGC 2 cut(s) 538, 882
Bme1390I CCNGG 4 cut(s) 857, 999, 1047, 1575
Bme18I GGWCC 1 cut(s) 1049
BmgT120I GGNCC 3 cut(s) 28, 702, 1049
BmiI GGNNCC 1 cut(s) 1572
BmrFI CCNGG 4 cut(s) 857, 999, 1047, 1575
BmrI ACTGGG 1 cut(s) 923
BmsI GCATC 1 cut(s) 715
BmtI GCTAGC 1 cut(s) 1179
BmuI ACTGGG 1 cut(s) 923
Bpu14I TTCGAA 1 cut(s) 1069
BpuEI CTTGAG 2 cut(s) 718, 1247
BpuMI CCSGG 2 cut(s) 857, 1575
Bsa29I ATCGAT 1 cut(s) 777
BsaBI GATNNNNATC 2 cut(s) 1035, 1361
BsaHI GRCGYC 1 cut(s) 1254
BsaJI CCNNGG 2 cut(s) 98, 705
Bsc4I CCNNNNNNNGG 4 cut(s) 756, 1312, 1409, 1483
Bse1I ACTGG 2 cut(s) 918, 1289
Bse8I GATNNNNATC 2 cut(s) 1035, 1361
BseBI CCWGG 2 cut(s) 999, 1047
BseCI ATCGAT 1 cut(s) 777
BseDI CCNNGG 2 cut(s) 98, 705
BseGI GGATG 2 cut(s) 121, 509
BseJI GATNNNNATC 2 cut(s) 1035, 1361
BseLI CCNNNNNNNGG 4 cut(s) 756, 1312, 1409, 1483
BseMII CTCAG 3 cut(s) 287, 1321, 1365
BseNI ACTGG 2 cut(s) 918, 1289
BsePI GCGCGC 1 cut(s) 1530
BseRI GAGGAG 6 cut(s) 71, 83, 86, 443, 539, 1499
BseXI GCAGC 2 cut(s) 523, 867
Bsh1236I CGCG 1 cut(s) 1532
BshFI GGCC 4 cut(s) 29, 704, 1237, 1305
BshVI ATCGAT 1 cut(s) 777
BsiSI CCGG 2 cut(s) 857, 1575
BslFI GGGAC 1 cut(s) 864
BslI CCNNNNNNNGG 4 cut(s) 756, 1312, 1409, 1483
BsmAI GTCTC 2 cut(s) 446, 1354
BsmFI GGGAC 1 cut(s) 864
BsnI GGCC 4 cut(s) 29, 704, 1237, 1305
Bsp119I TTCGAA 1 cut(s) 1069
Bsp1286I GDGCHC 1 cut(s) 1575
Bsp1407I TGTACA 1 cut(s) 1507
BspACI CCGC 1 cut(s) 290
BspANI GGCC 4 cut(s) 29, 704, 1237, 1305
BspCNI CTCAG 3 cut(s) 288, 1320, 1364
BspDI ATCGAT 1 cut(s) 777
BspFNI CGCG 1 cut(s) 1532
BspHI TCATGA 1 cut(s) 1020
BspLI GGNNCC 1 cut(s) 1572
BspMI ACCTGC 1 cut(s) 1075
BspOI GCTAGC 1 cut(s) 1179
BspPI GGATC 5 cut(s) 360, 942, 1316, 1532, 1549
BspQI GCTCTTC 2 cut(s) 153, 1101
BspT104I TTCGAA 1 cut(s) 1069
BsrGI TGTACA 1 cut(s) 1507
BsrI ACTGG 2 cut(s) 918, 1289
BssECI CCNNGG 2 cut(s) 98, 705
BssHII GCGCGC 1 cut(s) 1530
BssNAI GTATAC 1 cut(s) 1388
BssNI GRCGYC 1 cut(s) 1254
BssSI CACGAG 1 cut(s) 1663
BssT1I CCWWGG 2 cut(s) 98, 705
Bst1107I GTATAC 1 cut(s) 1388
Bst2BI CACGAG 1 cut(s) 1663
Bst2UI CCWGG 2 cut(s) 999, 1047
Bst6I CTCTTC 6 cut(s) 18, 48, 153, 432, 918, 1101
BstACI GRCGYC 1 cut(s) 1254
BstAUI TGTACA 1 cut(s) 1507
BstBI TTCGAA 1 cut(s) 1069
BstC8I GCNNGC 2 cut(s) 1177, 1532
BstDEI CTNAG 6 cut(s) 296, 812, 1056, 1307, 1351, 1566
BstENI CCTNNNNNAGG 1 cut(s) 1310
BstF5I GGATG 2 cut(s) 121, 509
BstFNI CGCG 1 cut(s) 1532
BstH2I RGCGCY 1 cut(s) 1182
BstHHI GCGC 3 cut(s) 1181, 1532, 1534
BstMAI GTCTC 2 cut(s) 446, 1354
BstMWI GCNNNNNNNGC 2 cut(s) 1172, 1406
BstNI CCWGG 2 cut(s) 999, 1047
BstNSI RCATGY 1 cut(s) 940
BstSCI CCNGG 4 cut(s) 855, 997, 1045, 1573
BstUI CGCG 1 cut(s) 1532
BstV1I GCAGC 2 cut(s) 523, 867
BstX2I RGATCY 2 cut(s) 947, 1356
BstYI RGATCY 2 cut(s) 947, 1356
BstZ17I GTATAC 1 cut(s) 1388
Bsu15I ATCGAT 1 cut(s) 777
BsuRI GGCC 4 cut(s) 29, 704, 1237, 1305
BsuTUI ATCGAT 1 cut(s) 777
BtsCI GGATG 2 cut(s) 121, 509
BtsIMutI CAGTG 1 cut(s) 1177
BveI ACCTGC 1 cut(s) 1075
Cac8I GCNNGC 2 cut(s) 1177, 1532
CciI TCATGA 1 cut(s) 1020
CfoI GCGC 3 cut(s) 1181, 1532, 1534
Cfr13I GGNCC 3 cut(s) 28, 702, 1049
ClaI ATCGAT 1 cut(s) 777
Csp6I GTAC 5 cut(s) 641, 911, 1159, 1267, 1508
CviAII CATG 4 cut(s) 272, 323, 937, 1021
CviQI GTAC 5 cut(s) 641, 911, 1159, 1267, 1508
DdeI CTNAG 6 cut(s) 296, 812, 1056, 1307, 1351, 1566
Eam1104I CTCTTC 6 cut(s) 18, 48, 153, 432, 918, 1101
EarI CTCTTC 6 cut(s) 18, 48, 153, 432, 918, 1101
EciI GGCGGA 1 cut(s) 305
Eco130I CCWWGG 2 cut(s) 98, 705
Eco24I GRGCYC 1 cut(s) 1575
Eco47I GGWCC 1 cut(s) 1049
Eco47III AGCGCT 1 cut(s) 1180
Eco57I CTGAAG 2 cut(s) 942, 1153
EcoNI CCTNNNNNAGG 1 cut(s) 1310
EcoRII CCWGG 2 cut(s) 997, 1045
EcoT14I CCWWGG 2 cut(s) 98, 705
EcoT38I GRGCYC 1 cut(s) 1575
ErhI CCWWGG 2 cut(s) 98, 705
FaeI CATG 4 cut(s) 275, 326, 940, 1024
FalI AAGNNNNNCTT 2 cut(s) 974, 1006
FaqI GGGAC 1 cut(s) 864
FatI CATG 4 cut(s) 271, 322, 936, 1020
FauNDI CATATG 1 cut(s) 1495
FbaI TGATCA 1 cut(s) 1023
FblI GTMKAC 1 cut(s) 1387
Fnu4HI GCNGC 2 cut(s) 537, 881
FokI GGATG 2 cut(s) 128, 496
FriOI GRGCYC 1 cut(s) 1575
Fsp4HI GCNGC 2 cut(s) 537, 881
FspBI CTAG 4 cut(s) 654, 1176, 1335, 1436
GlaI GCGC 3 cut(s) 1180, 1531, 1533
GluI GCNGC 2 cut(s) 537, 881
HaeII RGCGCY 1 cut(s) 1182
HaeIII GGCC 4 cut(s) 29, 704, 1237, 1305
HapII CCGG 2 cut(s) 857, 1575
HhaI GCGC 3 cut(s) 1181, 1532, 1534
Hin1I GRCGYC 1 cut(s) 1254
Hin1II CATG 4 cut(s) 275, 326, 940, 1024
Hin6I GCGC 3 cut(s) 1179, 1530, 1532
HinP1I GCGC 3 cut(s) 1179, 1530, 1532
HincII GTYRAC 1 cut(s) 1590
HindII GTYRAC 1 cut(s) 1590
HinfI GANTC 3 cut(s) 203, 779, 1245
HpaI GTTAAC 1 cut(s) 1590
HpaII CCGG 2 cut(s) 857, 1575
HphI GGTGA 3 cut(s) 73, 815, 1182
Hpy166II GTNNAC 4 cut(s) 721, 1388, 1456, 1590
Hpy188I TCNGA 7 cut(s) 202, 259, 981, 1310, 1354, 1381, 1550
Hpy8I GTNNAC 4 cut(s) 721, 1388, 1456, 1590
HpyCH4IV ACGT 1 cut(s) 1254
HpyCH4V TGCA 3 cut(s) 539, 629, 1127
HpyF10VI GCNNNNNNNGC 2 cut(s) 1172, 1406
HpyF3I CTNAG 6 cut(s) 296, 812, 1056, 1307, 1351, 1566
HpySE526I ACGT 1 cut(s) 1254
Hsp92I GRCGYC 1 cut(s) 1254
Hsp92II CATG 4 cut(s) 275, 326, 940, 1024
HspAI GCGC 3 cut(s) 1179, 1530, 1532
Ksp22I TGATCA 1 cut(s) 1023
KspAI GTTAAC 1 cut(s) 1590
LguI GCTCTTC 2 cut(s) 153, 1101
LmnI GCTCC 5 cut(s) 292, 1063, 1515, 1570, 1604
Lsp1109I GCAGC 2 cut(s) 523, 867
LweI GCATC 1 cut(s) 715
MaeI CTAG 4 cut(s) 654, 1176, 1335, 1436
MaeII ACGT 1 cut(s) 1254
MaeIII GTNAC 2 cut(s) 863, 1631
MflI RGATCY 2 cut(s) 947, 1356
MhlI GDGCHC 1 cut(s) 1575
MlyI GAGTC 1 cut(s) 1254
MmeI TCCRAC 2 cut(s) 163, 537
MseI TTAA 7 cut(s) 233, 338, 416, 740, 876, 993, 1589
MspA1I CMGCKG 1 cut(s) 581
MspI CCGG 2 cut(s) 857, 1575
MspR9I CCNGG 4 cut(s) 857, 999, 1047, 1575
MvaI CCWGG 2 cut(s) 999, 1047
MvnI CGCG 1 cut(s) 1532
MwoI GCNNNNNNNGC 2 cut(s) 1172, 1406
NciI CCSGG 2 cut(s) 857, 1575
NdeI CATATG 1 cut(s) 1495
NheI GCTAGC 1 cut(s) 1175
NlaIII CATG 4 cut(s) 275, 326, 940, 1024
NlaIV GGNNCC 1 cut(s) 1572
NmeAIII GCCGAG 1 cut(s) 1213
NspI RCATGY 1 cut(s) 940
NspV TTCGAA 1 cut(s) 1069
PagI TCATGA 1 cut(s) 1020
PaqCI CACCTGC 1 cut(s) 1075
PauI GCGCGC 1 cut(s) 1530
PciI ACATGT 1 cut(s) 936
PciSI GCTCTTC 2 cut(s) 153, 1101
PfeI GAWTC 2 cut(s) 203, 779
PkrI GCNGC 2 cut(s) 538, 882
PleI GAGTC 1 cut(s) 1253
PpsI GAGTC 1 cut(s) 1253
PscI ACATGT 1 cut(s) 936
PsiI TTATAA 1 cut(s) 944
Psp6I CCWGG 2 cut(s) 997, 1045
PspGI CCWGG 2 cut(s) 997, 1045
PspN4I GGNNCC 1 cut(s) 1572
PspPI GGNCC 3 cut(s) 28, 702, 1049
PsuI RGATCY 2 cut(s) 947, 1356
PteI GCGCGC 1 cut(s) 1530
PvuII CAGCTG 1 cut(s) 581
RsaI GTAC 5 cut(s) 642, 912, 1160, 1268, 1509
RsaNI GTAC 5 cut(s) 641, 911, 1159, 1267, 1508
SapI GCTCTTC 2 cut(s) 153, 1101
SaqAI TTAA 7 cut(s) 233, 338, 416, 740, 876, 993, 1589
SatI GCNGC 2 cut(s) 537, 881
Sau96I GGNCC 3 cut(s) 28, 702, 1049
SchI GAGTC 1 cut(s) 1254
ScrFI CCNGG 4 cut(s) 857, 999, 1047, 1575
SduI GDGCHC 1 cut(s) 1575
SfaNI GCATC 1 cut(s) 715
SfuI TTCGAA 1 cut(s) 1069
SinI GGWCC 1 cut(s) 1049
SmlI CTYRAG 2 cut(s) 697, 1262
SmoI CTYRAG 2 cut(s) 697, 1262
SsiI CCGC 1 cut(s) 290
SspI AATATT 1 cut(s) 1017
SspMI CTAG 4 cut(s) 654, 1176, 1335, 1436
StyD4I CCNGG 4 cut(s) 855, 997, 1045, 1573
StyI CCWWGG 2 cut(s) 98, 705
TaiI ACGT 1 cut(s) 1257
TaqI TCGA 6 cut(s) 302, 777, 782, 987, 1069, 1596
TatI WGTACW 2 cut(s) 1266, 1507
TfiI GAWTC 2 cut(s) 203, 779
Tru1I TTAA 7 cut(s) 233, 338, 416, 740, 876, 993, 1589
Tru9I TTAA 7 cut(s) 233, 338, 416, 740, 876, 993, 1589
TscAI CASTG 1 cut(s) 1177
TseI GCWGC 2 cut(s) 536, 880
TspDTI ATGAA 6 cut(s) 339, 906, 1009, 1088, 1213, 1482
TspRI CASTG 1 cut(s) 1177
VpaK11BI GGWCC 1 cut(s) 1049
XagI CCTNNNNNAGG 1 cut(s) 1310
XapI RAATTY 4 cut(s) 692, 1220, 1469, 1626
XceI RCATGY 1 cut(s) 940
XmiI GTMKAC 1 cut(s) 1387
XspI CTAG 4 cut(s) 654, 1176, 1335, 1436
ZraI GACGTC 1 cut(s) 1255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.