FvH4_3g11240

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001644
Physical Location & Seq
Forward (+)
6615801 .. 6618028
2228 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g11240.t1

Sequence Viewer

Length: 1569 bp
ATGACTGTTGAGGAGACCCAGGTGGCGGTGGCGGTGGAGGAGCCGGTGAAGGAGTGCGAGAAGGCTGTTGTTGCTGAGAAGCCAGCGAAAGTTGAGAAGAGCTCTTCTTACAAGGAGGAGAGCAACTATCTTTCTGATCTCAAGGAGTTCGAGAAGAAGGCTTTGGTTGAGCTCAAAGCCAAGCTCGAAGAAGCCATCTTGGGGAACAATATCTTCAAGAAAGATGAGCCCAAGAAGGTTGAGGAGAAGAAGGAGGAGAAAACAGAAGAGGCGGAGCCTGCTGCTGTGAAAACAGAGGAGGCAGAGCCTGCTGTGAAAACAGAGGAAACAGAGGCTGCTGTGAAAACAGAGGAAACAGAGGCTGCTGTGAAAACAGAGGAAACAGAGGCTGCTGTGAAAACAGAGTGTGCGGAGAAAACAGAGGAAGGAGAGAAGGCACCAGAATTGGCTGAGGAGAAAGAACAAGTGGTTGCTCCGGTATGTGAGGAAGAGGCGAAACCTGAATCCAGCGACGTGGTGGATACAGATATCTCACTCTGGGGTGTGCCTTTGTTCCCTGGTAAAGGGTCAGAGGGGACTGATGTGGTGCTGTTGAAATTCTTGAGGGCTAGGGAGTTCAAGGTGAACGAAGCCTTCGAGATGCTGAAGAAGACTCTGCAGTGGAGGAAGGACTCCAAAATTGATTCAATTTTGGAGGAGGATATCTGCTCTGATCTGAGCTCTGCCGCTTACTTGAACGGAGTTGATCGTGAAGGCCACCCTGTTTGCTACAACATCTTCGGAGTGTTTGACAGTGAAGAGCTTTACCAGAAGACGTTCGGGAATGAAGAGAAGAGAGGCCAATTCTTGAGGTGGAGGGTTCAGTTGATGGAGAAGAGTATTCAGAAGCTTGAATTGAGGCCTGGTGGTGCTACTTCTCTGCTTCAGATCAATGACCTCAAGAACTCGCCTGGACCGATCAAGAAGGAGCTTAGGGTTGCAACAAAGCAGGCTGTTGGGCTCTTGCAGGATAATTACCCTGAATTGGTTGCCAAAAATATTTTCATCAATGTTCCATTCTGGTACTATGCACTCAATGCTCTATTGTCTCCATTCTTGACTCAACGAACAAAGAGCAAGTTTGTGGTGGCTCGCCCCGCTAAGGTCACTGAGACCCTTCTCAAGTACATTCCAGCTGAGGAGATACCAATTCAGTATGGAGGCTTCAAAAGAGAAGATGACACAGAGTTCTTCTCTTCCGAAGATGGTTCTGTTTCCGAGCTAACTCTCAAGGCCGGATCAACCCAAACCATAGAGATTGAAGCAGCTGAGGCTGATAGTAGCAGCACATTGGTTTGGGATGTTAGTATTTTGGGATGGGAAGTGAATTACAAGGAGGAGTTTGTTCCAAGTGATGAAGGGTCATATACCATCATTGTTCAGAAGAAGAAGAAGATTGGAGCTAATGAAGGACCTATCCGCAACACTTTCCGAAGCAATGAGCCAGGGAAGGTTGTCCTGACAATTGAGAACACTTCGAGCAAGAAGAAGAGGGTTCTGTATCGGCACAAGACCAACAAGTGCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

523

Amino Acids

58.61

Weight (kDa)

4.93

Isoelectric Point (pI)

56.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_N PF03765 164 - 217 9.9e-08 CRAL/TRIO, N-terminal domain
CRAL_TRIO PF00650 242 - 401 1.4e-23 CRAL/TRIO domain
GOLD_PATL1_C PF25099 424 - 510 9.3e-36 Patellin-1-like, C-terminal GOLD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 436
AciI CCGC 7 cut(s) 26, 32, 272, 410, 726, 1137, 1459
AclWI GGATC 1 cut(s) 1285
AcsI RAATTY 1 cut(s) 596
AcuI CTGAAG 2 cut(s) 665, 908
AfaI GTAC 2 cut(s) 1064, 1166
AfiI CCNNNNNNNGG 5 cut(s) 25, 201, 563, 1024, 1141
AgsI TTSAA 8 cut(s) 217, 595, 619, 687, 736, 893, 1207, 1301
AjiI CACGTC 1 cut(s) 514
AjnI CCWGG 5 cut(s) 18, 556, 901, 949, 1483
AjuI GAANNNNNNNTTGG 4 cut(s) 146, 178, 1025, 1057
Alw21I GWGCWC 3 cut(s) 104, 174, 722
Alw26I GTCTC 3 cut(s) 8, 1092, 1145
AlwI GGATC 1 cut(s) 1285
AlwNI CAGNNNCTG 4 cut(s) 308, 335, 362, 389
AoxI GGCC 4 cut(s) 754, 838, 899, 1272
ApeKI GCWGC 6 cut(s) 281, 335, 362, 389, 1304, 1323
ApoI RAATTY 1 cut(s) 596
Asp700I GAANNNNTTC 1 cut(s) 815
AspS9I GGNCC 2 cut(s) 953, 1451
AsuHPI GGTGA 2 cut(s) 58, 634
AvaII GGWCC 2 cut(s) 953, 1451
BanI GGYRCC 1 cut(s) 436
BanII GRGCYC 5 cut(s) 104, 174, 231, 722, 1002
BarI GAAGNNNNNNTAC 2 cut(s) 1187, 1219
BbsI GAAGAC 2 cut(s) 656, 818
Bbv12I GWGCWC 3 cut(s) 104, 174, 722
BbvCI CCTCAGC 3 cut(s) 450, 1176, 1308
BbvI GCAGC 6 cut(s) 268, 322, 349, 376, 1316, 1335
BccI CCATC 5 cut(s) 203, 862, 1238, 1350, 1418
BciT130I CCWGG 5 cut(s) 20, 558, 903, 951, 1485
BciVI GTATCC 1 cut(s) 514
BcoDI GTCTC 3 cut(s) 8, 1092, 1145
BfaI CTAG 1 cut(s) 609
BfmI CTRYAG 1 cut(s) 656
BfuI GTATCC 1 cut(s) 514
BisI GCNGC 7 cut(s) 282, 336, 363, 390, 726, 1305, 1324
BlsI GCNGC 7 cut(s) 283, 337, 364, 391, 727, 1306, 1325
Bme1390I CCNGG 5 cut(s) 20, 558, 903, 951, 1485
Bme18I GGWCC 2 cut(s) 953, 1451
BmgBI CACGTC 1 cut(s) 514
BmgT120I GGNCC 2 cut(s) 953, 1451
BmiI GGNNCC 3 cut(s) 42, 276, 438
BmrFI CCNGG 5 cut(s) 20, 558, 903, 951, 1485
BmsI GCATC 1 cut(s) 630
BpiI GAAGAC 2 cut(s) 656, 818
BplI GAGNNNNNCTC 4 cut(s) 86, 118, 1217, 1249
Bpu10I CCTNAGC 5 cut(s) 450, 971, 1140, 1176, 1308
BpuEI CTTGAG 6 cut(s) 125, 622, 868, 923, 1145, 1253
BsaI GGTCTC 2 cut(s) 8, 1145
BsaJI CCNNGG 3 cut(s) 18, 556, 1484
BsaWI WCCGGW 1 cut(s) 475
Bsc4I CCNNNNNNNGG 5 cut(s) 25, 201, 563, 1024, 1141
Bse118I RCCGGY 1 cut(s) 43
Bse3DI GCAATG 1 cut(s) 1483
BseBI CCWGG 5 cut(s) 20, 558, 903, 951, 1485
BseDI CCNNGG 3 cut(s) 18, 556, 1484
BseGI GGATG 2 cut(s) 1345, 1361
BseLI CCNNNNNNNGG 5 cut(s) 25, 201, 563, 1024, 1141
BseMI GCAATG 1 cut(s) 1483
BseMII CTCAG 6 cut(s) 66, 441, 707, 1140, 1167, 1299
BseXI GCAGC 6 cut(s) 268, 322, 349, 376, 1316, 1335
BshFI GGCC 4 cut(s) 756, 840, 901, 1274
BshNI GGYRCC 1 cut(s) 436
BsiHKAI GWGCWC 3 cut(s) 104, 174, 722
BsiSI CCGG 3 cut(s) 44, 476, 1275
BslFI GGGAC 1 cut(s) 589
BslI CCNNNNNNNGG 5 cut(s) 25, 201, 563, 1024, 1141
BsmAI GTCTC 3 cut(s) 8, 1092, 1145
BsmFI GGGAC 1 cut(s) 589
BsnI GGCC 4 cut(s) 756, 840, 901, 1274
Bso31I GGTCTC 2 cut(s) 8, 1145
Bsp1286I GDGCHC 5 cut(s) 104, 174, 231, 722, 1002
Bsp143I GATC 6 cut(s) 136, 712, 745, 927, 957, 1277
BspACI CCGC 7 cut(s) 26, 32, 272, 410, 726, 1137, 1459
BspANI GGCC 4 cut(s) 756, 840, 901, 1274
BspCNI CTCAG 6 cut(s) 67, 442, 708, 1141, 1168, 1300
BspLI GGNNCC 3 cut(s) 42, 276, 438
BspMAI CTGCAG 1 cut(s) 660
BspPI GGATC 1 cut(s) 1285
BspQI GCTCTTC 3 cut(s) 92, 109, 792
BspT107I GGYRCC 1 cut(s) 436
BspTNI GGTCTC 2 cut(s) 8, 1145
BsrDI GCAATG 1 cut(s) 1483
BsrFI RCCGGY 1 cut(s) 43
BssAI RCCGGY 1 cut(s) 43
BssECI CCNNGG 3 cut(s) 18, 556, 1484
BssMI GATC 6 cut(s) 136, 712, 745, 927, 957, 1277
Bst2UI CCWGG 5 cut(s) 20, 558, 903, 951, 1485
Bst4CI ACNGT 2 cut(s) 7, 794
BstAPI GCANNNNNTGC 2 cut(s) 308, 1076
BstC8I GCNNGC 5 cut(s) 84, 279, 309, 990, 1132
BstDEI CTNAG 8 cut(s) 75, 450, 716, 971, 1140, 1149, 1176, 1308
BstENI CCTNNNNNAGG 1 cut(s) 561
BstF5I GGATG 2 cut(s) 1345, 1361
BstKTI GATC 6 cut(s) 139, 715, 748, 930, 960, 1280
BstMAI GTCTC 3 cut(s) 8, 1092, 1145
BstMBI GATC 6 cut(s) 136, 712, 745, 927, 957, 1277
BstMWI GCNNNNNNNGC 6 cut(s) 71, 278, 308, 1076, 1136, 1310
BstNI CCWGG 5 cut(s) 20, 558, 903, 951, 1485
BstSCI CCNGG 5 cut(s) 18, 556, 901, 949, 1483
BstSFI CTRYAG 1 cut(s) 656
BstV1I GCAGC 6 cut(s) 268, 322, 349, 376, 1316, 1335
BstV2I GAAGAC 2 cut(s) 656, 818
BstXI CCANNNNNNTGG 1 cut(s) 514
BsuI GTATCC 1 cut(s) 514
BsuRI GGCC 4 cut(s) 756, 840, 901, 1274
BtrI CACGTC 1 cut(s) 514
BtsCI GGATG 2 cut(s) 1345, 1361
BtsI GCAGTG 1 cut(s) 665
BtsIMutI CAGTG 3 cut(s) 665, 799, 1146
Cac8I GCNNGC 5 cut(s) 84, 279, 309, 990, 1132
CaiI CAGNNNCTG 4 cut(s) 308, 335, 362, 389
Cfr10I RCCGGY 1 cut(s) 43
Cfr13I GGNCC 2 cut(s) 953, 1451
Csp6I GTAC 2 cut(s) 1063, 1165
CspCI CAANNNNNGTGG 2 cut(s) 746, 781
CviQI GTAC 2 cut(s) 1063, 1165
DdeI CTNAG 8 cut(s) 75, 450, 716, 971, 1140, 1149, 1176, 1308
DpnI GATC 6 cut(s) 138, 714, 747, 929, 959, 1279
DpnII GATC 6 cut(s) 136, 712, 745, 927, 957, 1277
EciI GGCGGA 1 cut(s) 287
Ecl136II GAGCTC 3 cut(s) 102, 172, 720
Eco147I AGGCCT 1 cut(s) 901
Eco24I GRGCYC 5 cut(s) 104, 174, 231, 722, 1002
Eco31I GGTCTC 2 cut(s) 8, 1145
Eco32I GATATC 2 cut(s) 529, 703
Eco47I GGWCC 2 cut(s) 953, 1451
Eco53kI GAGCTC 3 cut(s) 102, 172, 720
Eco57I CTGAAG 2 cut(s) 665, 908
EcoICRI GAGCTC 3 cut(s) 102, 172, 720
EcoNI CCTNNNNNAGG 1 cut(s) 561
EcoO109I RGGNCCY 1 cut(s) 1451
EcoRII CCWGG 5 cut(s) 18, 556, 901, 949, 1483
EcoRV GATATC 2 cut(s) 529, 703
EcoT38I GRGCYC 5 cut(s) 104, 174, 231, 722, 1002
FaiI YATR 6 cut(s) 481, 1068, 1197, 1292, 1405, 1407
FaqI GGGAC 1 cut(s) 589
FauI CCCGC 1 cut(s) 1144
Fnu4HI GCNGC 7 cut(s) 282, 336, 363, 390, 726, 1305, 1324
FokI GGATG 2 cut(s) 1352, 1368
FriOI GRGCYC 5 cut(s) 104, 174, 231, 722, 1002
Fsp4HI GCNGC 7 cut(s) 282, 336, 363, 390, 726, 1305, 1324
FspBI CTAG 1 cut(s) 609
GluI GCNGC 7 cut(s) 282, 336, 363, 390, 726, 1305, 1324
HaeIII GGCC 4 cut(s) 756, 840, 901, 1274
HapII CCGG 3 cut(s) 44, 476, 1275
HindIII AAGCTT 1 cut(s) 887
HinfI GANTC 5 cut(s) 503, 652, 671, 683, 1099
HpaII CCGG 3 cut(s) 44, 476, 1275
HphI GGTGA 2 cut(s) 58, 634
Hpy166II GTNNAC 1 cut(s) 625
Hpy8I GTNNAC 1 cut(s) 625
Hpy99I CGWCG 1 cut(s) 515
HpyCH4III ACNGT 2 cut(s) 7, 794
HpyCH4IV ACGT 2 cut(s) 513, 815
HpyCH4V TGCA 4 cut(s) 658, 980, 1006, 1070
HpyF10VI GCNNNNNNNGC 6 cut(s) 71, 278, 308, 1076, 1136, 1310
HpyF3I CTNAG 8 cut(s) 75, 450, 716, 971, 1140, 1149, 1176, 1308
HpySE526I ACGT 2 cut(s) 513, 815
Kzo9I GATC 6 cut(s) 136, 712, 745, 927, 957, 1277
LguI GCTCTTC 3 cut(s) 92, 109, 792
LmnI GCTCC 5 cut(s) 40, 274, 478, 967, 1439
Lsp1109I GCAGC 6 cut(s) 268, 322, 349, 376, 1316, 1335
LweI GCATC 1 cut(s) 630
MaeI CTAG 1 cut(s) 609
MaeII ACGT 2 cut(s) 513, 815
MaeIII GTNAC 1 cut(s) 1144
MalI GATC 6 cut(s) 138, 714, 747, 929, 959, 1279
MboI GATC 6 cut(s) 136, 712, 745, 927, 957, 1277
MfeI CAATTG 1 cut(s) 1503
MhlI GDGCHC 5 cut(s) 104, 174, 231, 722, 1002
MlyI GAGTC 3 cut(s) 646, 665, 1093
MroXI GAANNNNTTC 1 cut(s) 815
MspA1I CMGCKG 2 cut(s) 1175, 1307
MspI CCGG 3 cut(s) 44, 476, 1275
MspR9I CCNGG 5 cut(s) 20, 558, 903, 951, 1485
MunI CAATTG 1 cut(s) 1503
MvaI CCWGG 5 cut(s) 20, 558, 903, 951, 1485
MwoI GCNNNNNNNGC 6 cut(s) 71, 278, 308, 1076, 1136, 1310
NdeII GATC 6 cut(s) 136, 712, 745, 927, 957, 1277
NlaIV GGNNCC 3 cut(s) 42, 276, 438
NmuCI GTSAC 1 cut(s) 1144
PceI AGGCCT 1 cut(s) 901
PciSI GCTCTTC 3 cut(s) 92, 109, 792
PcsI WCGNNNNNNNCGW 2 cut(s) 633, 953
PdmI GAANNNNTTC 1 cut(s) 815
PfeI GAWTC 2 cut(s) 503, 683
PkrI GCNGC 7 cut(s) 283, 337, 364, 391, 727, 1306, 1325
PleI GAGTC 3 cut(s) 646, 665, 1093
PpsI GAGTC 3 cut(s) 646, 665, 1093
PpuMI RGGWCCY 1 cut(s) 1451
Psp124BI GAGCTC 3 cut(s) 104, 174, 722
Psp5II RGGWCCY 1 cut(s) 1451
Psp6I CCWGG 5 cut(s) 18, 556, 901, 949, 1483
PspGI CCWGG 5 cut(s) 18, 556, 901, 949, 1483
PspN4I GGNNCC 3 cut(s) 42, 276, 438
PspPI GGNCC 2 cut(s) 953, 1451
PspPPI RGGWCCY 1 cut(s) 1451
PstI CTGCAG 1 cut(s) 660
PstNI CAGNNNCTG 4 cut(s) 308, 335, 362, 389
PvuII CAGCTG 2 cut(s) 1175, 1307
RsaI GTAC 2 cut(s) 1064, 1166
RsaNI GTAC 2 cut(s) 1063, 1165
SacI GAGCTC 3 cut(s) 104, 174, 722
SapI GCTCTTC 3 cut(s) 92, 109, 792
SatI GCNGC 7 cut(s) 282, 336, 363, 390, 726, 1305, 1324
Sau3AI GATC 6 cut(s) 136, 712, 745, 927, 957, 1277
Sau96I GGNCC 2 cut(s) 953, 1451
SchI GAGTC 3 cut(s) 646, 665, 1093
ScrFI CCNGG 5 cut(s) 20, 558, 903, 951, 1485
SduI GDGCHC 5 cut(s) 104, 174, 231, 722, 1002
SfaNI GCATC 1 cut(s) 630
SfcI CTRYAG 1 cut(s) 656
SinI GGWCC 2 cut(s) 953, 1451
SmlI CTYRAG 6 cut(s) 140, 601, 847, 938, 1160, 1268
SmoI CTYRAG 6 cut(s) 140, 601, 847, 938, 1160, 1268
SseBI AGGCCT 1 cut(s) 901
SsiI CCGC 7 cut(s) 26, 32, 272, 410, 726, 1137, 1459
SspI AATATT 1 cut(s) 1039
SspMI CTAG 1 cut(s) 609
SstI GAGCTC 3 cut(s) 104, 174, 722
StuI AGGCCT 1 cut(s) 901
StyD4I CCNGG 5 cut(s) 18, 556, 901, 949, 1483
TaaI ACNGT 2 cut(s) 7, 794
TaiI ACGT 2 cut(s) 516, 818
TaqI TCGA 4 cut(s) 150, 186, 636, 1517
TaqII GACCGA 1 cut(s) 970
TatI WGTACW 1 cut(s) 1164
TauI GCSGC 1 cut(s) 728
TfiI GAWTC 2 cut(s) 503, 683
TscAI CASTG 3 cut(s) 665, 799, 1153
TseFI GTSAC 1 cut(s) 1144
TseI GCWGC 6 cut(s) 281, 335, 362, 389, 1304, 1323
Tsp45I GTSAC 1 cut(s) 1144
TspDTI ATGAA 4 cut(s) 840, 1033, 1410, 1461
TspGWI ACGGA 1 cut(s) 753
TspRI CASTG 3 cut(s) 665, 799, 1153
VpaK11BI GGWCC 2 cut(s) 953, 1451
XagI CCTNNNNNAGG 1 cut(s) 561
XapI RAATTY 1 cut(s) 596
XcmI CCANNNNNNNNNTGG 1 cut(s) 514
XmnI GAANNNNTTC 1 cut(s) 815
XspI CTAG 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.