pycom05g25000

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001644
Physical Location & Seq
Forward (+)
26447344 .. 26449330
1987 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g25000.1

Sequence Viewer

Length: 1569 bp
ATGACTGCGGAGGAGACCCAGGTGGCGGAAGTTGTGGTTGTTCCTCAGGAGGAGGCTGAGAAGGTTGTGTGTGAGAATGAGAAGGTTGTTGAGGGAGATGATAAGGTGAAGGAAGTGGATGAGGCGGAATCGAAGCCGAAAGCGGTTGTGAAGAGCTCGTCTTATAAGGAAGAGAGCAACTATCTCTCCGATTTGAAGGAGTTCGAGAAGAAGGCGTTGAGCGAGCTGAAATCAAAGCTGGAGGAGGCCATTTTGGGCAACAATATCTTCAAGGAGAAGGAGCCAAAGAAGGAGGAGGAGAAGAAGGAAGAGAAAAAAGTAGCGGAGGAAGAGAAGAAGGAAGATAAAGCAGAGGAGGGAGAAAAAATAGAGGAGGCAGTGAAAACAGAGGAGGAGGAGAAGAAGGAAGAGAAACAGGAGGAGGAATGTGAAAAAAAGACGGAGGAAGAAGCTGAAGAGAATGAGAAGACGGCGGAAGAAGGCGAGGAGGCGAAGAAACCGGAAGACAGGTGTGAAGTAGTAGACGAGGATATCTCTCTTTGGGGAGTGCCACTTTTCCCCGGTAAAGGTTTCGAGGGCACTGATGTTGTTCTCTTGAAATTCTTAAGGGCTAGGGAGTTCAAGGTCAATGAGGCTTTCGAGATGCTGAAGAAAACCCTTCAATGGCGGAAGGAGTCCAAGATCGATGCGATTTTGGACGAGGATGTCTGTGCCGATCTGAGCTCTGCCGCTTACTTGAACGGCGTCGATCGCGAAGGCCACCCCGTTTGCTACAACATTTTCGGGGTATTCGACAACGAGGAGCTTTATCAGAAGACTTTTGGAAATGAGGAGAAGAGAGGGCAGTTCCTGAGGTGGAGGCTCCAGCTGATGGAGAAGAGCGTACAGAAGCTTGATCTGAGGCCTGGTGGTGTCACTTCTCTGCTTCAGATCAACGACCTCAAGAACTCTCCCGGGCCGGTGAAGAAGGAGCTCCGGATTGCCACAAAGCAAGCTGTTGGGCTTCTGCAGGACAACTACCCTGAGTTGGTTGCCAAAAATATCTTCATAAATGTTCCTTTCTGGTATTATGCACTTAATGCATTGCTATCTCCTTTCTTGACGCAAAGAACCAAGAGCAAGTTCGTCGTTGCTCGCCCCGCGAAGGTCACTGAAACCCTTCTCAAGTACATTCCGGCTCAGGAGATACCTGCTCAGTATGGCGGTTTCAAGAGGGACAACGACAACGAGTTCTCTGCCGAAGATGGCGCTGTTTCGGAACTTATTCTTAGGGCTGGATCCACTGGAACCATCCAAATTGATGTAGCAGAGGTTGGTAGCACATTAGTGTGGGACTTGACTGTTTTGGGATGGGAAGTGAATTACAAGGAGGAGTTTGTTCCAACTGATGAGGAGTCGTACACCATTATTGTTCAGAAGAAGAAGAAAATGGGGTCTAACGAAGGCCCAATTCGCAACACTTTCCGAAGCAACGAATCCGGGAAGGTTGTCCTGACAATCGAGAACAATTCGAGCAAGAAGAAGAGGGTTCTGTATCGGTACAAGGCCAACAAGTGCTCCACCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

523

Amino Acids

59.56

Weight (kDa)

4.91

Isoelectric Point (pI)

52.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 243 - 402 2e-23 CRAL/TRIO domain
GOLD_PATL1_C PF25099 424 - 508 4.7e-36 Patellin-1-like, C-terminal GOLD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 165
AasI GACNNNNNNGTC 1 cut(s) 704
Acc36I ACCTGC 1 cut(s) 1198
AccB7I CCANNNNNTGG 1 cut(s) 871
AccI GTMKAC 1 cut(s) 522
AccII CGCG 2 cut(s) 753, 1142
AccIII TCCGGA 1 cut(s) 975
AclWI GGATC 2 cut(s) 1272, 1285
AcsI RAATTY 1 cut(s) 599
AcuI CTGAAG 3 cut(s) 474, 668, 911
AcyI GRCGYC 1 cut(s) 744
AfaI GTAC 4 cut(s) 885, 1169, 1400, 1541
AfiI CCNNNNNNNGG 6 cut(s) 25, 566, 663, 871, 1027, 1144
AflII CTTAAG 1 cut(s) 604
AgsI TTSAA 7 cut(s) 196, 271, 598, 622, 662, 739, 1210
AjnI CCWGG 2 cut(s) 18, 904
AjuI GAANNNNNNNTTGG 4 cut(s) 1028, 1060, 1106, 1138
AleI CACNNNNGTG 1 cut(s) 1325
Alw21I GWGCWC 4 cut(s) 158, 725, 975, 1559
Alw26I GTCTC 1 cut(s) 8
AlwI GGATC 2 cut(s) 1272, 1285
AlwNI CAGNNNCTG 1 cut(s) 850
Ama87I CYCGRG 1 cut(s) 953
Aor13HI TCCGGA 1 cut(s) 975
AoxI GGCC 6 cut(s) 246, 757, 902, 956, 1444, 1545
ApoI RAATTY 1 cut(s) 599
Asp700I GAANNNNTTC 3 cut(s) 200, 1158, 1263
AspLEI GCGC 1 cut(s) 1250
AspS9I GGNCC 2 cut(s) 956, 1445
AsuC2I CCSGG 4 cut(s) 561, 954, 955, 1480
AsuHPI GGTGA 2 cut(s) 118, 973
AvaI CYCGRG 1 cut(s) 953
AxyI CCTNAGG 2 cut(s) 45, 851
BaeGI GKGCMC 1 cut(s) 581
BamHI GGATCC 1 cut(s) 1277
BanII GRGCYC 3 cut(s) 158, 725, 975
BbsI GAAGAC 3 cut(s) 473, 510, 821
Bbv12I GWGCWC 4 cut(s) 158, 725, 975, 1559
BccI CCATC 4 cut(s) 865, 1238, 1298, 1344
BceAI ACGGC 2 cut(s) 486, 757
BcgI CGANNNNNNTGC 4 cut(s) 1108, 1142, 1217, 1251
BciT130I CCWGG 2 cut(s) 20, 906
BcnI CCSGG 4 cut(s) 561, 954, 955, 1480
BcoDI GTCTC 1 cut(s) 8
BfaI CTAG 1 cut(s) 612
BfmI CTRYAG 1 cut(s) 1007
BfoI RGCGCY 1 cut(s) 1251
BfrI CTTAAG 1 cut(s) 604
BfuAI ACCTGC 1 cut(s) 1198
BisI GCNGC 1 cut(s) 729
BlsI GCNGC 1 cut(s) 730
Bme1390I CCNGG 6 cut(s) 20, 561, 906, 954, 955, 1480
BmeT110I CYCGRG 1 cut(s) 953
BmgT120I GGNCC 2 cut(s) 956, 1445
BmiI GGNNCC 4 cut(s) 282, 863, 1279, 1288
BmrFI CCNGG 6 cut(s) 20, 561, 906, 954, 955, 1480
BmsI GCATC 2 cut(s) 633, 676
BpiI GAAGAC 3 cut(s) 473, 510, 821
BplI GAGNNNNNCTC 2 cut(s) 518, 550
BpmI CTGGAG 2 cut(s) 260, 848
Bpu10I CCTNAGC 1 cut(s) 1179
BpuEI CTTGAG 2 cut(s) 926, 1148
BpuMI CCSGG 4 cut(s) 561, 954, 955, 1480
Bsa29I ATCGAT 1 cut(s) 684
BsaHI GRCGYC 1 cut(s) 744
BsaI GGTCTC 1 cut(s) 8
BsaJI CCNNGG 3 cut(s) 18, 559, 953
BsaWI WCCGGW 2 cut(s) 499, 975
BsaXI ACNNNNNCTCC 3 cut(s) 170, 200, 1541
Bsc4I CCNNNNNNNGG 6 cut(s) 25, 566, 663, 871, 1027, 1144
Bse118I RCCGGY 1 cut(s) 958
Bse1I ACTGG 1 cut(s) 1288
Bse21I CCTNAGG 2 cut(s) 45, 851
Bse3DI GCAATG 1 cut(s) 1082
BseAI TCCGGA 1 cut(s) 975
BseBI CCWGG 2 cut(s) 20, 906
BseCI ATCGAT 1 cut(s) 684
BseDI CCNNGG 3 cut(s) 18, 559, 953
BseGI GGATG 4 cut(s) 124, 709, 1290, 1355
BseLI CCNNNNNNNGG 6 cut(s) 25, 566, 663, 871, 1027, 1144
BseMI GCAATG 1 cut(s) 1082
BseMII CTCAG 8 cut(s) 48, 59, 710, 842, 890, 1014, 1193, 1208
BseNI ACTGG 1 cut(s) 1288
BseSI GKGCMC 1 cut(s) 581
Bsh1236I CGCG 2 cut(s) 753, 1142
Bsh1285I CGRYCG 1 cut(s) 751
BshFI GGCC 6 cut(s) 248, 759, 904, 958, 1446, 1547
BshVI ATCGAT 1 cut(s) 684
BsiEI CGRYCG 1 cut(s) 751
BsiHKAI GWGCWC 4 cut(s) 158, 725, 975, 1559
BsiHKCI CYCGRG 1 cut(s) 953
BsiSI CCGG 7 cut(s) 500, 561, 954, 959, 976, 1175, 1479
BslFI GGGAC 2 cut(s) 1229, 1346
BslI CCNNNNNNNGG 6 cut(s) 25, 566, 663, 871, 1027, 1144
BsmAI GTCTC 1 cut(s) 8
BsmFI GGGAC 2 cut(s) 1229, 1346
BsnI GGCC 6 cut(s) 248, 759, 904, 958, 1446, 1547
Bso31I GGTCTC 1 cut(s) 8
BsoBI CYCGRG 1 cut(s) 953
Bsp1286I GDGCHC 5 cut(s) 158, 581, 725, 975, 1559
Bsp13I TCCGGA 1 cut(s) 975
Bsp143I GATC 6 cut(s) 681, 715, 748, 895, 930, 1277
Bsp68I TCGCGA 1 cut(s) 753
BspANI GGCC 6 cut(s) 248, 759, 904, 958, 1446, 1547
BspCNI CTCAG 8 cut(s) 49, 58, 711, 843, 891, 1015, 1192, 1207
BspDI ATCGAT 1 cut(s) 684
BspEI TCCGGA 1 cut(s) 975
BspFNI CGCG 2 cut(s) 753, 1142
BspLI GGNNCC 4 cut(s) 282, 863, 1279, 1288
BspMAI CTGCAG 1 cut(s) 1011
BspMI ACCTGC 1 cut(s) 1198
BspPI GGATC 2 cut(s) 1272, 1285
BspQI GCTCTTC 2 cut(s) 146, 872
BspTI CTTAAG 1 cut(s) 604
BspTNI GGTCTC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 1082
BsrFI RCCGGY 1 cut(s) 958
BsrI ACTGG 1 cut(s) 1288
BssAI RCCGGY 1 cut(s) 958
BssECI CCNNGG 3 cut(s) 18, 559, 953
BssMI GATC 6 cut(s) 681, 715, 748, 895, 930, 1277
BssNI GRCGYC 1 cut(s) 744
Bst2UI CCWGG 2 cut(s) 20, 906
Bst4CI ACNGT 1 cut(s) 1342
Bst6I CTCTTC 9 cut(s) 146, 165, 303, 324, 402, 450, 830, 872, 1517
BstACI GRCGYC 1 cut(s) 744
BstAFI CTTAAG 1 cut(s) 604
BstAPI GCANNNNNTGC 1 cut(s) 1079
BstC8I GCNNGC 3 cut(s) 224, 993, 1135
BstDEI CTNAG 9 cut(s) 45, 57, 719, 851, 899, 1023, 1179, 1194, 1268
BstF5I GGATG 4 cut(s) 124, 709, 1290, 1355
BstFNI CGCG 2 cut(s) 753, 1142
BstH2I RGCGCY 1 cut(s) 1251
BstHHI GCGC 1 cut(s) 1250
BstKTI GATC 6 cut(s) 684, 718, 751, 898, 933, 1280
BstMAI GTCTC 1 cut(s) 8
BstMBI GATC 6 cut(s) 681, 715, 748, 895, 930, 1277
BstMCI CGRYCG 1 cut(s) 751
BstMWI GCNNNNNNNGC 4 cut(s) 750, 1079, 1139, 1452
BstNI CCWGG 2 cut(s) 20, 906
BstSCI CCNGG 6 cut(s) 18, 559, 904, 952, 953, 1478
BstSFI CTRYAG 1 cut(s) 1007
BstSLI GKGCMC 1 cut(s) 581
BstUI CGCG 2 cut(s) 753, 1142
BstV2I GAAGAC 3 cut(s) 473, 510, 821
BstX2I RGATCY 1 cut(s) 1277
BstYI RGATCY 1 cut(s) 1277
Bsu15I ATCGAT 1 cut(s) 684
Bsu36I CCTNAGG 2 cut(s) 45, 851
BsuRI GGCC 6 cut(s) 248, 759, 904, 958, 1446, 1547
BsuTUI ATCGAT 1 cut(s) 684
BtsCI GGATG 4 cut(s) 124, 709, 1290, 1355
BtsI GCAGTG 1 cut(s) 384
BtsIMutI CAGTG 4 cut(s) 384, 579, 1149, 1281
BtuMI TCGCGA 1 cut(s) 753
BveI ACCTGC 1 cut(s) 1198
Cac8I GCNNGC 3 cut(s) 224, 993, 1135
CaiI CAGNNNCTG 1 cut(s) 850
CfoI GCGC 1 cut(s) 1250
Cfr10I RCCGGY 1 cut(s) 958
Cfr13I GGNCC 2 cut(s) 956, 1445
Cfr9I CCCGGG 1 cut(s) 953
ClaI ATCGAT 1 cut(s) 684
CseI GACGC 2 cut(s) 733, 1111
Csp6I GTAC 4 cut(s) 884, 1168, 1399, 1540
CspCI CAANNNNNGTGG 2 cut(s) 749, 784
CviQI GTAC 4 cut(s) 884, 1168, 1399, 1540
DdeI CTNAG 9 cut(s) 45, 57, 719, 851, 899, 1023, 1179, 1194, 1268
DpnI GATC 6 cut(s) 683, 717, 750, 897, 932, 1279
DpnII GATC 6 cut(s) 681, 715, 748, 895, 930, 1277
DrdI GACNNNNNNGTC 1 cut(s) 704
DseDI GACNNNNNNGTC 1 cut(s) 704
Eam1104I CTCTTC 9 cut(s) 146, 165, 303, 324, 402, 450, 830, 872, 1517
EarI CTCTTC 9 cut(s) 146, 165, 303, 324, 402, 450, 830, 872, 1517
EciI GGCGGA 4 cut(s) 41, 140, 488, 682
Ecl136II GAGCTC 3 cut(s) 156, 723, 973
Eco147I AGGCCT 1 cut(s) 904
Eco24I GRGCYC 3 cut(s) 158, 725, 975
Eco31I GGTCTC 1 cut(s) 8
Eco32I GATATC 1 cut(s) 532
Eco53kI GAGCTC 3 cut(s) 156, 723, 973
Eco57I CTGAAG 3 cut(s) 474, 668, 911
Eco81I CCTNAGG 2 cut(s) 45, 851
Eco88I CYCGRG 1 cut(s) 953
EcoICRI GAGCTC 3 cut(s) 156, 723, 973
EcoRII CCWGG 2 cut(s) 18, 904
EcoRV GATATC 1 cut(s) 532
EcoT22I ATGCAT 1 cut(s) 1084
EcoT38I GRGCYC 3 cut(s) 158, 725, 975
FaiI YATR 4 cut(s) 165, 1049, 1071, 1200
FaqI GGGAC 2 cut(s) 1229, 1346
FauI CCCGC 1 cut(s) 1147
FblI GTMKAC 1 cut(s) 522
Fnu4HI GCNGC 1 cut(s) 729
FokI GGATG 4 cut(s) 131, 716, 1277, 1362
FriOI GRGCYC 3 cut(s) 158, 725, 975
Fsp4HI GCNGC 1 cut(s) 729
FspBI CTAG 1 cut(s) 612
GlaI GCGC 1 cut(s) 1249
GluI GCNGC 1 cut(s) 729
GsuI CTGGAG 2 cut(s) 260, 848
HaeII RGCGCY 1 cut(s) 1251
HaeIII GGCC 6 cut(s) 248, 759, 904, 958, 1446, 1547
HapII CCGG 7 cut(s) 500, 561, 954, 959, 976, 1175, 1479
HgaI GACGC 2 cut(s) 733, 1111
HhaI GCGC 1 cut(s) 1250
Hin1I GRCGYC 1 cut(s) 744
Hin6I GCGC 1 cut(s) 1248
HinP1I GCGC 1 cut(s) 1248
HindIII AAGCTT 1 cut(s) 890
HinfI GANTC 4 cut(s) 128, 674, 1394, 1475
HpaII CCGG 7 cut(s) 500, 561, 954, 959, 976, 1175, 1479
HphI GGTGA 2 cut(s) 118, 973
Hpy166II GTNNAC 2 cut(s) 523, 1401
Hpy188I TCNGA 9 cut(s) 190, 720, 813, 900, 930, 1258, 1416, 1466, 1568
Hpy8I GTNNAC 2 cut(s) 523, 1401
Hpy99I CGWCG 2 cut(s) 749, 1130
HpyCH4III ACNGT 1 cut(s) 1342
HpyCH4V TGCA 3 cut(s) 1009, 1073, 1082
HpyF10VI GCNNNNNNNGC 4 cut(s) 750, 1079, 1139, 1452
HpyF3I CTNAG 9 cut(s) 45, 57, 719, 851, 899, 1023, 1179, 1194, 1268
Hsp92I GRCGYC 1 cut(s) 744
HspAI GCGC 1 cut(s) 1248
Kpn2I TCCGGA 1 cut(s) 975
Kzo9I GATC 6 cut(s) 681, 715, 748, 895, 930, 1277
LguI GCTCTTC 2 cut(s) 146, 872
LmnI GCTCC 6 cut(s) 280, 802, 867, 970, 978, 1562
LweI GCATC 2 cut(s) 633, 676
MaeI CTAG 1 cut(s) 612
MaeIII GTNAC 2 cut(s) 913, 1147
MalI GATC 6 cut(s) 683, 717, 750, 897, 932, 1279
MboI GATC 6 cut(s) 681, 715, 748, 895, 930, 1277
MflI RGATCY 1 cut(s) 1277
MhlI GDGCHC 5 cut(s) 158, 581, 725, 975, 1559
MluCI AATT 5 cut(s) 599, 1296, 1360, 1449, 1507
MlyI GAGTC 2 cut(s) 683, 1403
MmeI TCCRAC 1 cut(s) 1406
Mph1103I ATGCAT 1 cut(s) 1084
MroI TCCGGA 1 cut(s) 975
MroXI GAANNNNTTC 3 cut(s) 200, 1158, 1263
MseI TTAA 2 cut(s) 605, 1077
MslI CAYNNNNRTG 2 cut(s) 1325, 1327
MspA1I CMGCKG 1 cut(s) 868
MspCI CTTAAG 1 cut(s) 604
MspI CCGG 7 cut(s) 500, 561, 954, 959, 976, 1175, 1479
MspR9I CCNGG 6 cut(s) 20, 561, 906, 954, 955, 1480
MvaI CCWGG 2 cut(s) 20, 906
MvnI CGCG 2 cut(s) 753, 1142
MwoI GCNNNNNNNGC 4 cut(s) 750, 1079, 1139, 1452
NciI CCSGG 4 cut(s) 561, 954, 955, 1480
NdeII GATC 6 cut(s) 681, 715, 748, 895, 930, 1277
NlaIV GGNNCC 4 cut(s) 282, 863, 1279, 1288
NmuCI GTSAC 2 cut(s) 913, 1147
NruI TCGCGA 1 cut(s) 753
NsiI ATGCAT 1 cut(s) 1084
OliI CACNNNNGTG 1 cut(s) 1325
PceI AGGCCT 1 cut(s) 904
PciSI GCTCTTC 2 cut(s) 146, 872
PcsI WCGNNNNNNNCGW 1 cut(s) 789
PdmI GAANNNNTTC 3 cut(s) 200, 1158, 1263
PfeI GAWTC 2 cut(s) 128, 1475
PflMI CCANNNNNTGG 1 cut(s) 871
PfoI TCCNGGA 1 cut(s) 1478
PkrI GCNGC 1 cut(s) 730
Ple19I CGATCG 1 cut(s) 751
PleI GAGTC 2 cut(s) 682, 1402
PpsI GAGTC 2 cut(s) 682, 1402
PsiI TTATAA 1 cut(s) 165
Psp124BI GAGCTC 3 cut(s) 158, 725, 975
Psp6I CCWGG 2 cut(s) 18, 904
PspGI CCWGG 2 cut(s) 18, 904
PspN4I GGNNCC 4 cut(s) 282, 863, 1279, 1288
PspPI GGNCC 2 cut(s) 956, 1445
PstI CTGCAG 1 cut(s) 1011
PstNI CAGNNNCTG 1 cut(s) 850
PsuI RGATCY 1 cut(s) 1277
PvuI CGATCG 1 cut(s) 751
PvuII CAGCTG 1 cut(s) 868
RruI TCGCGA 1 cut(s) 753
RsaI GTAC 4 cut(s) 885, 1169, 1400, 1541
RsaNI GTAC 4 cut(s) 884, 1168, 1399, 1540
RseI CAYNNNNRTG 2 cut(s) 1325, 1327
SacI GAGCTC 3 cut(s) 158, 725, 975
SapI GCTCTTC 2 cut(s) 146, 872
SaqAI TTAA 2 cut(s) 605, 1077
SatI GCNGC 1 cut(s) 729
Sau3AI GATC 6 cut(s) 681, 715, 748, 895, 930, 1277
Sau96I GGNCC 2 cut(s) 956, 1445
SchI GAGTC 2 cut(s) 683, 1403
ScrFI CCNGG 6 cut(s) 20, 561, 906, 954, 955, 1480
SduI GDGCHC 5 cut(s) 158, 581, 725, 975, 1559
SfaNI GCATC 2 cut(s) 633, 676
SfcI CTRYAG 1 cut(s) 1007
SmaI CCCGGG 1 cut(s) 955
SmiMI CAYNNNNRTG 2 cut(s) 1325, 1327
SmlI CTYRAG 3 cut(s) 604, 941, 1163
SmoI CTYRAG 3 cut(s) 604, 941, 1163
Sse9I AATT 5 cut(s) 599, 1296, 1360, 1449, 1507
SseBI AGGCCT 1 cut(s) 904
SspMI CTAG 1 cut(s) 612
SstI GAGCTC 3 cut(s) 158, 725, 975
StuI AGGCCT 1 cut(s) 904
StyD4I CCNGG 6 cut(s) 18, 559, 904, 952, 953, 1478
TaaI ACNGT 1 cut(s) 1342
TaqI TCGA 9 cut(s) 131, 204, 573, 639, 684, 747, 792, 1500, 1511
TasI AATT 5 cut(s) 599, 1296, 1360, 1449, 1507
TatI WGTACW 1 cut(s) 1167
TauI GCSGC 1 cut(s) 731
TfiI GAWTC 2 cut(s) 128, 1475
Tru1I TTAA 2 cut(s) 605, 1077
Tru9I TTAA 2 cut(s) 605, 1077
TscAI CASTG 4 cut(s) 384, 586, 1156, 1288
TseFI GTSAC 2 cut(s) 913, 1147
Tsp45I GTSAC 2 cut(s) 913, 1147
TspDTI ATGAA 1 cut(s) 1036
TspGWI ACGGA 1 cut(s) 455
TspMI CCCGGG 1 cut(s) 953
TspRI CASTG 4 cut(s) 384, 586, 1156, 1288
Van91I CCANNNNNTGG 1 cut(s) 871
Vha464I CTTAAG 1 cut(s) 604
XapI RAATTY 1 cut(s) 599
XmaI CCCGGG 1 cut(s) 953
XmiI GTMKAC 1 cut(s) 522
XmnI GAANNNNTTC 3 cut(s) 200, 1158, 1263
XspI CTAG 1 cut(s) 612
Zsp2I ATGCAT 1 cut(s) 1084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.