pycom05g18370

Patellin-4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001644
Physical Location & Seq
Forward (+)
21329613 .. 21331616
2004 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g18370.4

Sequence Viewer

Length: 1368 bp
ATGACGGAGAAGAAAAAGGAGGAGGAGACACCATTAGAGAATGTCGAGGATGATCGTGTCGAAGAAACCGAGGATGAAAGCACCCAAGAAGAAGGAAGAAAAAATAGCCCAAGTGAAGATCAAGAAAGTGATGTAGAAAAGGAGGGTGGAAATGTAACTTCACCGTCTGTCGAATTCAAGAACAAGAAGAAGAAAAGGCAGAGAGCCTTGATGGAATTGAGGTGCAGGGTTGAGGATGCAATTCTTGGGAATTATCTGTTGGGAAAACCCAACAAGAAGCTTGGATCGGACGAGAGTTTTAGGCGGGAGAAGGAGCCAAGGGAGATCACTCTTTGGGGGGTGCCCTTACTCCCTAGCAAAGGCCACCAAGGCACCGACATTGTGCTGCTCAAGTTCTTGAGAGCGAAAGACTACAAGGTCCTCGAAGCGTTCGAGATGCTGCAAAGGATGCTGAAATGGCGAAGAGAATACAAAACGGATGAGATTCTGGAGGAGGAGTTGGGGTGTGATCTTAAGACTGTTGTGTTTTTGAATAGTGTGGACAAGGAAGGGCACCCTCTGTGTTATACATTTTATGGGCCCTTTAGAGATAGGGAATTGTACAGTAGGACTTTTGGTTCTGAGGAGAAACGGCAAGAGTTTTTGAGGTGGAGGGTTCAGTTTATGGAAAGGGGAATTCAAAAACTTAGCTTCAAGGACGGAGGAGTGGATTCTATGGTTCACGTTACCGATTTGAAGCACTCGCCAGGGCCGGACATGAAAGAGCTTCGTTCTCTTAGCAACAAAACCCTAGTTTTGCTCCAAGAAAACTATCCAGAGCTCATCCAAAAGAATATTGTGATAAATGCTCCACTTTGGTATTATGTGGTGCATGTGCTCAGATCAAGGCTCCTAAGTCAAAGAACCAAAAAGAAGTTTGTGTTTGCCAGGCCTTCAAATGTTACAAAAACCCTTCTCAAGTTTGTAGAACCAGAAGAACTCCCAGCTCAGTATGGTGGCCTCAAAAGAGAGGAAGATGATGATTTCACACCAGAGGACAAAGCATCAAGGCTCAAAGTCAAACCAAGCACAAATGCCTGCATCGAAATCCCAGTTGCTGAGGGTGGATTGACAATGGTGTGGGATCTAACAGTGGTGGGATGGGATGTGTACTACAAGGAGGAGTTTGTACCTGATGATGAAGGCTCATACAAGATTCTGCTCCAAGAAAGGAAGAGATTGGGACAAAGTGTTAGAAACTCCTTCTATATTAGTGAACCTGGCAAGATGGTGATAACAATTGAGAACTGGACATTCAAGAACAAGAGGGTTCTCTATAGGTCCAAAGCCAAACCCACTCTCCCCATGTATATCTACTTTAACAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

456

Amino Acids

53.28

Weight (kDa)

7.57

Isoelectric Point (pI)

60.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 416
AccB1I GGYRCC 3 cut(s) 340, 371, 552
AciI CCGC 1 cut(s) 304
AclWI GGATC 2 cut(s) 292, 1131
AcsI RAATTY 2 cut(s) 173, 675
AfaI GTAC 3 cut(s) 602, 1151, 1170
AfiI CCNNNNNNNGG 1 cut(s) 359
AflII CTTAAG 1 cut(s) 512
AgsI TTSAA 7 cut(s) 178, 532, 680, 694, 736, 936, 1297
AjnI CCWGG 3 cut(s) 745, 926, 1258
AjuI GAANNNNNNNTTGG 2 cut(s) 242, 274
AloI GAACNNNNNNTCC 2 cut(s) 601, 633
AluBI AGCT 5 cut(s) 280, 690, 766, 820, 986
AluI AGCT 5 cut(s) 280, 690, 766, 820, 986
Alw21I GWGCWC 2 cut(s) 822, 879
Alw26I GTCTC 1 cut(s) 20
AlwI GGATC 2 cut(s) 292, 1131
AlwNI CAGNNNCTG 1 cut(s) 1097
AoxI GGCC 5 cut(s) 361, 578, 749, 929, 997
ApaI GGGCCC 1 cut(s) 582
ApeKI GCWGC 2 cut(s) 385, 439
ApoI RAATTY 2 cut(s) 173, 675
AspS9I GGNCC 5 cut(s) 418, 578, 579, 749, 1320
AsuHPI GGTGA 2 cut(s) 153, 1282
AvaII GGWCC 2 cut(s) 418, 1320
BaeGI GKGCMC 3 cut(s) 345, 555, 582
BanI GGYRCC 3 cut(s) 340, 371, 552
BanII GRGCYC 2 cut(s) 582, 822
Bbv12I GWGCWC 2 cut(s) 822, 879
BbvCI CCTCAGC 1 cut(s) 1098
BbvI GCAGC 2 cut(s) 372, 426
BccI CCATC 3 cut(s) 205, 1134, 1261
BceAI ACGGC 1 cut(s) 647
BciT130I CCWGG 3 cut(s) 747, 928, 1260
BcoDI GTCTC 1 cut(s) 20
BfaI CTAG 2 cut(s) 354, 791
BfmI CTRYAG 1 cut(s) 1315
BfrI CTTAAG 1 cut(s) 512
BglI GCCNNNNNGGC 1 cut(s) 369
BisI GCNGC 2 cut(s) 386, 440
BlsI GCNGC 2 cut(s) 387, 441
Bme1390I CCNGG 3 cut(s) 747, 928, 1260
Bme18I GGWCC 2 cut(s) 418, 1320
BmgT120I GGNCC 5 cut(s) 418, 578, 579, 749, 1320
BmiI GGNNCC 6 cut(s) 315, 342, 373, 554, 580, 890
BmrFI CCNGG 3 cut(s) 747, 928, 1260
BmrI ACTGGG 1 cut(s) 1085
BmsI GCATC 5 cut(s) 226, 426, 438, 1052, 1089
BmuI ACTGGG 1 cut(s) 1085
BpmI CTGGAG 1 cut(s) 509
Bpu10I CCTNAGC 1 cut(s) 1098
BpuEI CTTGAG 3 cut(s) 374, 418, 941
BsaJI CCNNGG 4 cut(s) 69, 317, 367, 746
BsaXI ACNNNNNCTCC 6 cut(s) 482, 488, 512, 518, 1323, 1353
Bsc4I CCNNNNNNNGG 1 cut(s) 359
Bse1I ACTGG 2 cut(s) 1091, 1292
BseBI CCWGG 3 cut(s) 747, 928, 1260
BseDI CCNNGG 4 cut(s) 69, 317, 367, 746
BseGI GGATG 8 cut(s) 55, 79, 241, 453, 484, 822, 1145, 1150
BseLI CCNNNNNNNGG 1 cut(s) 359
BseMII CTCAG 4 cut(s) 612, 892, 1001, 1089
BseNI ACTGG 2 cut(s) 1091, 1292
BseRI GAGGAG 7 cut(s) 35, 38, 506, 509, 638, 717, 1175
BseSI GKGCMC 3 cut(s) 345, 555, 582
BseXI GCAGC 2 cut(s) 372, 426
BseYI CCCAGC 1 cut(s) 982
BsgI GTGCAG 1 cut(s) 244
BshFI GGCC 5 cut(s) 363, 580, 751, 931, 999
BshNI GGYRCC 3 cut(s) 340, 371, 552
BsiHKAI GWGCWC 2 cut(s) 822, 879
BsiSI CCGG 1 cut(s) 752
BslFI GGGAC 1 cut(s) 1236
BslI CCNNNNNNNGG 1 cut(s) 359
BsmAI GTCTC 1 cut(s) 20
BsmFI GGGAC 1 cut(s) 1236
BsnI GGCC 5 cut(s) 363, 580, 751, 931, 999
Bsp120I GGGCCC 1 cut(s) 578
Bsp1286I GDGCHC 5 cut(s) 345, 555, 582, 822, 879
Bsp1407I TGTACA 1 cut(s) 600
Bsp143I GATC 7 cut(s) 52, 118, 284, 324, 508, 881, 1123
BspACI CCGC 1 cut(s) 304
BspANI GGCC 5 cut(s) 363, 580, 751, 931, 999
BspCNI CTCAG 4 cut(s) 613, 891, 1000, 1090
BspLI GGNNCC 6 cut(s) 315, 342, 373, 554, 580, 890
BspPI GGATC 2 cut(s) 292, 1131
BspT107I GGYRCC 3 cut(s) 340, 371, 552
BspTI CTTAAG 1 cut(s) 512
BsrGI TGTACA 1 cut(s) 600
BsrI ACTGG 2 cut(s) 1091, 1292
BssECI CCNNGG 4 cut(s) 69, 317, 367, 746
BssMI GATC 7 cut(s) 52, 118, 284, 324, 508, 881, 1123
BssT1I CCWWGG 2 cut(s) 317, 367
Bst2UI CCWGG 3 cut(s) 747, 928, 1260
Bst4CI ACNGT 4 cut(s) 165, 520, 605, 1132
Bst6I CTCTTC 2 cut(s) 457, 1208
BstAFI CTTAAG 1 cut(s) 512
BstAPI GCANNNNNTGC 1 cut(s) 448
BstAUI TGTACA 1 cut(s) 600
BstC8I GCNNGC 1 cut(s) 1078
BstDEI CTNAG 7 cut(s) 621, 686, 776, 878, 893, 987, 1098
BstENI CCTNNNNNAGG 1 cut(s) 357
BstF5I GGATG 8 cut(s) 55, 79, 241, 453, 484, 822, 1145, 1150
BstKTI GATC 7 cut(s) 55, 121, 287, 327, 511, 884, 1126
BstMAI GTCTC 1 cut(s) 20
BstMBI GATC 7 cut(s) 52, 118, 284, 324, 508, 881, 1123
BstMWI GCNNNNNNNGC 3 cut(s) 369, 448, 457
BstNI CCWGG 3 cut(s) 747, 928, 1260
BstNSI RCATGY 1 cut(s) 875
BstSCI CCNGG 3 cut(s) 745, 926, 1258
BstSFI CTRYAG 1 cut(s) 1315
BstSLI GKGCMC 3 cut(s) 345, 555, 582
BstV1I GCAGC 2 cut(s) 372, 426
BstX2I RGATCY 1 cut(s) 1123
BstYI RGATCY 1 cut(s) 1123
BsuRI GGCC 5 cut(s) 363, 580, 751, 931, 999
BtsCI GGATG 8 cut(s) 55, 79, 241, 453, 484, 822, 1145, 1150
BtsIMutI CAGTG 1 cut(s) 1137
Cac8I GCNNGC 1 cut(s) 1078
CaiI CAGNNNCTG 1 cut(s) 1097
Cfr13I GGNCC 5 cut(s) 418, 578, 579, 749, 1320
Csp6I GTAC 3 cut(s) 601, 1150, 1169
CviAII CATG 3 cut(s) 757, 872, 1345
CviQI GTAC 3 cut(s) 601, 1150, 1169
DdeI CTNAG 7 cut(s) 621, 686, 776, 878, 893, 987, 1098
DpnI GATC 7 cut(s) 54, 120, 286, 326, 510, 883, 1125
DpnII GATC 7 cut(s) 52, 118, 284, 324, 508, 881, 1123
DrdI GACNNNNNNGTC 1 cut(s) 416
DseDI GACNNNNNNGTC 1 cut(s) 416
Eam1104I CTCTTC 2 cut(s) 457, 1208
EarI CTCTTC 2 cut(s) 457, 1208
Ecl136II GAGCTC 1 cut(s) 820
Eco130I CCWWGG 2 cut(s) 317, 367
Eco147I AGGCCT 1 cut(s) 931
Eco24I GRGCYC 2 cut(s) 582, 822
Eco47I GGWCC 2 cut(s) 418, 1320
Eco53kI GAGCTC 1 cut(s) 820
EcoICRI GAGCTC 1 cut(s) 820
EcoNI CCTNNNNNAGG 1 cut(s) 357
EcoO109I RGGNCCY 2 cut(s) 418, 579
EcoRI GAATTC 2 cut(s) 173, 675
EcoRII CCWGG 3 cut(s) 745, 926, 1258
EcoT14I CCWWGG 2 cut(s) 317, 367
EcoT38I GRGCYC 2 cut(s) 582, 822
ErhI CCWWGG 2 cut(s) 317, 367
FaeI CATG 3 cut(s) 760, 875, 1348
FaqI GGGAC 1 cut(s) 1236
FatI CATG 3 cut(s) 756, 871, 1344
FauI CCCGC 1 cut(s) 297
Fnu4HI GCNGC 2 cut(s) 386, 440
FokI GGATG 8 cut(s) 62, 86, 248, 460, 491, 809, 1152, 1157
FriOI GRGCYC 2 cut(s) 582, 822
Fsp4HI GCNGC 2 cut(s) 386, 440
FspBI CTAG 2 cut(s) 354, 791
GluI GCNGC 2 cut(s) 386, 440
GsaI CCCAGC 1 cut(s) 986
GsuI CTGGAG 1 cut(s) 509
HaeIII GGCC 5 cut(s) 363, 580, 751, 931, 999
HapII CCGG 1 cut(s) 752
Hin1II CATG 3 cut(s) 760, 875, 1348
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 3 cut(s) 484, 710, 1195
HpaII CCGG 1 cut(s) 752
HphI GGTGA 2 cut(s) 153, 1282
Hpy166II GTNNAC 4 cut(s) 541, 721, 1150, 1256
Hpy188I TCNGA 3 cut(s) 289, 622, 881
Hpy188III TCNNGA 7 cut(s) 122, 178, 397, 433, 488, 815, 1297
Hpy8I GTNNAC 4 cut(s) 541, 721, 1150, 1256
HpyAV CCTTC 7 cut(s) 86, 304, 542, 942, 962, 1175, 1252
HpyCH4III ACNGT 4 cut(s) 165, 520, 605, 1132
HpyCH4IV ACGT 1 cut(s) 723
HpyCH4V TGCA 5 cut(s) 225, 239, 442, 871, 1080
HpyF10VI GCNNNNNNNGC 3 cut(s) 369, 448, 457
HpyF3I CTNAG 7 cut(s) 621, 686, 776, 878, 893, 987, 1098
HpySE526I ACGT 1 cut(s) 723
Hsp92II CATG 3 cut(s) 760, 875, 1348
Kzo9I GATC 7 cut(s) 52, 118, 284, 324, 508, 881, 1123
LmnI GCTCC 5 cut(s) 313, 804, 853, 894, 1206
Lsp1109I GCAGC 2 cut(s) 372, 426
LweI GCATC 5 cut(s) 226, 426, 438, 1052, 1089
MaeI CTAG 2 cut(s) 354, 791
MaeII ACGT 1 cut(s) 723
MaeIII GTNAC 3 cut(s) 154, 724, 940
MalI GATC 7 cut(s) 54, 120, 286, 326, 510, 883, 1125
MboI GATC 7 cut(s) 52, 118, 284, 324, 508, 881, 1123
MfeI CAATTG 1 cut(s) 1278
MflI RGATCY 1 cut(s) 1123
MhlI GDGCHC 5 cut(s) 345, 555, 582, 822, 879
MluCI AATT 7 cut(s) 173, 215, 240, 250, 596, 675, 1278
MseI TTAA 2 cut(s) 513, 1359
MspCI CTTAAG 1 cut(s) 512
MspI CCGG 1 cut(s) 752
MspR9I CCNGG 3 cut(s) 747, 928, 1260
MunI CAATTG 1 cut(s) 1278
MvaI CCWGG 3 cut(s) 747, 928, 1260
MwoI GCNNNNNNNGC 3 cut(s) 369, 448, 457
NdeII GATC 7 cut(s) 52, 118, 284, 324, 508, 881, 1123
NlaIII CATG 3 cut(s) 760, 875, 1348
NlaIV GGNNCC 6 cut(s) 315, 342, 373, 554, 580, 890
NspI RCATGY 1 cut(s) 875
PceI AGGCCT 1 cut(s) 931
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 429
PfeI GAWTC 3 cut(s) 484, 710, 1195
PkrI GCNGC 2 cut(s) 387, 441
PpuMI RGGWCCY 1 cut(s) 418
Psp124BI GAGCTC 1 cut(s) 822
Psp5II RGGWCCY 1 cut(s) 418
Psp6I CCWGG 3 cut(s) 745, 926, 1258
PspFI CCCAGC 1 cut(s) 982
PspGI CCWGG 3 cut(s) 745, 926, 1258
PspN4I GGNNCC 6 cut(s) 315, 342, 373, 554, 580, 890
PspOMI GGGCCC 1 cut(s) 578
PspPI GGNCC 5 cut(s) 418, 578, 579, 749, 1320
PspPPI RGGWCCY 1 cut(s) 418
PstNI CAGNNNCTG 1 cut(s) 1097
PsuI RGATCY 1 cut(s) 1123
RsaI GTAC 3 cut(s) 602, 1151, 1170
RsaNI GTAC 3 cut(s) 601, 1150, 1169
SacI GAGCTC 1 cut(s) 822
SaqAI TTAA 2 cut(s) 513, 1359
SatI GCNGC 2 cut(s) 386, 440
Sau3AI GATC 7 cut(s) 52, 118, 284, 324, 508, 881, 1123
Sau96I GGNCC 5 cut(s) 418, 578, 579, 749, 1320
ScrFI CCNGG 3 cut(s) 747, 928, 1260
SduI GDGCHC 5 cut(s) 345, 555, 582, 822, 879
SfaNI GCATC 5 cut(s) 226, 426, 438, 1052, 1089
SfcI CTRYAG 1 cut(s) 1315
SinI GGWCC 2 cut(s) 418, 1320
SmlI CTYRAG 4 cut(s) 389, 397, 512, 956
SmoI CTYRAG 4 cut(s) 389, 397, 512, 956
Sse9I AATT 7 cut(s) 173, 215, 240, 250, 596, 675, 1278
SseBI AGGCCT 1 cut(s) 931
SsiI CCGC 1 cut(s) 304
SspI AATATT 1 cut(s) 835
SspMI CTAG 2 cut(s) 354, 791
SstI GAGCTC 1 cut(s) 822
StuI AGGCCT 1 cut(s) 931
StyD4I CCNGG 3 cut(s) 745, 926, 1258
StyI CCWWGG 2 cut(s) 317, 367
TaaI ACNGT 4 cut(s) 165, 520, 605, 1132
TaiI ACGT 1 cut(s) 726
TaqI TCGA 6 cut(s) 45, 60, 171, 423, 432, 1083
TasI AATT 7 cut(s) 173, 215, 240, 250, 596, 675, 1278
TatI WGTACW 2 cut(s) 600, 1149
TfiI GAWTC 3 cut(s) 484, 710, 1195
Tru1I TTAA 2 cut(s) 513, 1359
Tru9I TTAA 2 cut(s) 513, 1359
TscAI CASTG 1 cut(s) 1137
TseI GCWGC 2 cut(s) 385, 439
TspDTI ATGAA 3 cut(s) 90, 773, 1194
TspGWI ACGGA 3 cut(s) 20, 491, 714
TspRI CASTG 1 cut(s) 1137
Vha464I CTTAAG 1 cut(s) 512
VpaK11BI GGWCC 2 cut(s) 418, 1320
XagI CCTNNNNNAGG 1 cut(s) 357
XapI RAATTY 2 cut(s) 173, 675
XceI RCATGY 1 cut(s) 875
XspI CTAG 2 cut(s) 354, 791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.