CRAL/TRIO, N-terminal domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1529 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1358 |
| AatII | GACGTC | 1 cut(s) | 1197 |
| Acc36I | ACCTGC | 1 cut(s) | 1660 |
| Acc65I | GGTACC | 1 cut(s) | 1225 |
| AccB1I | GGYRCC | 2 cut(s) | 1225, 1421 |
| AccI | GTMKAC | 1 cut(s) | 1485 |
| AciI | CCGC | 6 cut(s) | 233, 521, 548, 557, 1887, 1897 |
| AclI | AACGTT | 1 cut(s) | 2166 |
| AclWI | GGATC | 3 cut(s) | 1901, 2083, 2197 |
| AcsI | RAATTY | 6 cut(s) | 634, 967, 1703, 1805, 1913, 2054 |
| AcuI | CTGAAG | 5 cut(s) | 327, 594, 1158, 1670, 1944 |
| AcyI | GRCGYC | 1 cut(s) | 1194 |
| AfaI | GTAC | 5 cut(s) | 1227, 1611, 1745, 1986, 2225 |
| AfeI | AGCGCT | 1 cut(s) | 1759 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 58, 391, 1244, 1828, 1994 |
| AflII | CTTAAG | 1 cut(s) | 1892 |
| AgeI | ACCGGT | 2 cut(s) | 1873, 2220 |
| AjnI | CCWGG | 4 cut(s) | 1582, 1630, 1826, 2158 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 680, 712 |
| AleI | CACNNNNGTG | 1 cut(s) | 1339 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1860, 1892 |
| Alw26I | GTCTC | 1 cut(s) | 485 |
| AlwI | GGATC | 3 cut(s) | 1901, 2083, 2197 |
| Ama87I | CYCGRG | 1 cut(s) | 734 |
| Aor51HI | AGCGCT | 1 cut(s) | 1759 |
| AoxI | GGCC | 2 cut(s) | 1144, 1633 |
| ApeKI | GCWGC | 4 cut(s) | 128, 1465, 1859, 1931 |
| ApoI | RAATTY | 6 cut(s) | 634, 967, 1703, 1805, 1913, 2054 |
| AsiGI | ACCGGT | 2 cut(s) | 1873, 2220 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1971, 2058 |
| Asp718I | GGTACC | 1 cut(s) | 1225 |
| AspLEI | GCGC | 1 cut(s) | 1760 |
| AspS9I | GGNCC | 2 cut(s) | 1634, 1876 |
| AsuHPI | GGTGA | 7 cut(s) | 40, 152, 221, 232, 446, 554, 1400 |
| AsuII | TTCGAA | 1 cut(s) | 1299 |
| AsuNHI | GCTAGC | 1 cut(s) | 1760 |
| AvaI | CYCGRG | 1 cut(s) | 734 |
| AvaII | GGWCC | 1 cut(s) | 1876 |
| BanI | GGYRCC | 2 cut(s) | 1225, 1421 |
| BbsI | GAAGAC | 1 cut(s) | 1804 |
| BbvI | GCAGC | 4 cut(s) | 140, 1452, 1846, 1918 |
| BccI | CCATC | 3 cut(s) | 85, 404, 1412 |
| BceAI | ACGGC | 2 cut(s) | 1302, 1500 |
| BciT130I | CCWGG | 4 cut(s) | 1584, 1632, 1828, 2160 |
| BclI | TGATCA | 2 cut(s) | 583, 2196 |
| BcoDI | GTCTC | 1 cut(s) | 485 |
| BfaI | CTAG | 3 cut(s) | 1239, 1290, 1761 |
| BfmI | CTRYAG | 2 cut(s) | 1753, 1860 |
| BfoI | RGCGCY | 1 cut(s) | 1761 |
| BfrI | CTTAAG | 1 cut(s) | 1892 |
| BfuAI | ACCTGC | 1 cut(s) | 1660 |
| BisI | GCNGC | 4 cut(s) | 129, 1466, 1860, 1932 |
| BlsI | GCNGC | 4 cut(s) | 130, 1467, 1861, 1933 |
| Bme1390I | CCNGG | 4 cut(s) | 1584, 1632, 1828, 2160 |
| Bme18I | GGWCC | 1 cut(s) | 1876 |
| BmeT110I | CYCGRG | 1 cut(s) | 734 |
| BmgT120I | GGNCC | 2 cut(s) | 1634, 1876 |
| BmiI | GGNNCC | 4 cut(s) | 1227, 1402, 1423, 1636 |
| BmrFI | CCNGG | 4 cut(s) | 1584, 1632, 1828, 2160 |
| BmrI | ACTGGG | 1 cut(s) | 1976 |
| BmsI | GCATC | 6 cut(s) | 487, 592, 1300, 1447, 1485, 1918 |
| BmtI | GCTAGC | 1 cut(s) | 1764 |
| BmuI | ACTGGG | 1 cut(s) | 1976 |
| BpiI | GAAGAC | 1 cut(s) | 1804 |
| BpmI | CTGGAG | 1 cut(s) | 1322 |
| Bpu14I | TTCGAA | 1 cut(s) | 1299 |
| BpuEI | CTTGAG | 2 cut(s) | 1604, 1832 |
| BsaHI | GRCGYC | 1 cut(s) | 1194 |
| BsaJI | CCNNGG | 4 cut(s) | 81, 327, 375, 1827 |
| BsaWI | WCCGGW | 3 cut(s) | 1441, 1873, 2220 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2098, 2128 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 58, 391, 1244, 1828, 1994 |
| Bse118I | RCCGGY | 2 cut(s) | 1873, 2220 |
| Bse1I | ACTGG | 3 cut(s) | 1339, 1425, 1982 |
| BseBI | CCWGG | 4 cut(s) | 1584, 1632, 1828, 2160 |
| BseDI | CCNNGG | 4 cut(s) | 81, 327, 375, 1827 |
| BseGI | GGATG | 8 cut(s) | 77, 385, 415, 517, 607, 1381, 1423, 1438 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 58, 391, 1244, 1828, 1994 |
| BseMII | CTCAG | 3 cut(s) | 48, 698, 1950 |
| BseNI | ACTGG | 3 cut(s) | 1339, 1425, 1982 |
| BseRI | GAGGAG | 5 cut(s) | 200, 203, 494, 566, 1549 |
| BseXI | GCAGC | 4 cut(s) | 140, 1452, 1846, 1918 |
| BseYI | CCCAGC | 1 cut(s) | 1498 |
| BshFI | GGCC | 2 cut(s) | 1146, 1635 |
| BshNI | GGYRCC | 2 cut(s) | 1225, 1421 |
| BshTI | ACCGGT | 2 cut(s) | 1873, 2220 |
| BsiHKCI | CYCGRG | 1 cut(s) | 734 |
| BsiSI | CCGG | 3 cut(s) | 1442, 1874, 2221 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 58, 391, 1244, 1828, 1994 |
| BsmAI | GTCTC | 1 cut(s) | 485 |
| BsnI | GGCC | 2 cut(s) | 1146, 1635 |
| BsoBI | CYCGRG | 1 cut(s) | 734 |
| Bsp119I | TTCGAA | 1 cut(s) | 1299 |
| Bsp19I | CCATGG | 1 cut(s) | 327 |
| BspACI | CCGC | 6 cut(s) | 233, 521, 548, 557, 1887, 1897 |
| BspANI | GGCC | 2 cut(s) | 1146, 1635 |
| BspCNI | CTCAG | 3 cut(s) | 49, 699, 1949 |
| BspLI | GGNNCC | 4 cut(s) | 1227, 1402, 1423, 1636 |
| BspMAI | CTGCAG | 1 cut(s) | 1864 |
| BspMI | ACCTGC | 1 cut(s) | 1660 |
| BspOI | GCTAGC | 1 cut(s) | 1764 |
| BspPI | GGATC | 3 cut(s) | 1901, 2083, 2197 |
| BspT104I | TTCGAA | 1 cut(s) | 1299 |
| BspT107I | GGYRCC | 2 cut(s) | 1225, 1421 |
| BspTI | CTTAAG | 1 cut(s) | 1892 |
| BsrFI | RCCGGY | 2 cut(s) | 1873, 2220 |
| BsrI | ACTGG | 3 cut(s) | 1339, 1425, 1982 |
| BssAI | RCCGGY | 2 cut(s) | 1873, 2220 |
| BssECI | CCNNGG | 4 cut(s) | 81, 327, 375, 1827 |
| BssNAI | GTATAC | 1 cut(s) | 1486 |
| BssNI | GRCGYC | 1 cut(s) | 1194 |
| BssT1I | CCWWGG | 3 cut(s) | 81, 327, 375 |
| Bst1107I | GTATAC | 1 cut(s) | 1486 |
| Bst2UI | CCWGG | 4 cut(s) | 1584, 1632, 1828, 2160 |
| Bst4CI | ACNGT | 2 cut(s) | 842, 2179 |
| Bst6I | CTCTTC | 6 cut(s) | 60, 561, 1011, 1062, 1473, 1503 |
| BstACI | GRCGYC | 1 cut(s) | 1194 |
| BstAFI | CTTAAG | 1 cut(s) | 1892 |
| BstBI | TTCGAA | 1 cut(s) | 1299 |
| BstC8I | GCNNGC | 4 cut(s) | 1659, 1762, 1819, 1889 |
| BstDEI | CTNAG | 5 cut(s) | 57, 707, 1606, 1641, 1936 |
| BstDSI | CCRYGG | 1 cut(s) | 327 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1242 |
| BstF5I | GGATG | 8 cut(s) | 77, 385, 415, 517, 607, 1381, 1423, 1438 |
| BstH2I | RGCGCY | 1 cut(s) | 1761 |
| BstHHI | GCGC | 1 cut(s) | 1760 |
| BstMAI | GTCTC | 1 cut(s) | 485 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 428, 554, 1757 |
| BstNI | CCWGG | 4 cut(s) | 1584, 1632, 1828, 2160 |
| BstNSI | RCATGY | 1 cut(s) | 1630 |
| BstSCI | CCNGG | 4 cut(s) | 1582, 1630, 1826, 2158 |
| BstSFI | CTRYAG | 2 cut(s) | 1753, 1860 |
| BstV1I | GCAGC | 4 cut(s) | 140, 1452, 1846, 1918 |
| BstV2I | GAAGAC | 1 cut(s) | 1804 |
| BstZ17I | GTATAC | 1 cut(s) | 1486 |
| BsuRI | GGCC | 2 cut(s) | 1146, 1635 |
| BtgI | CCRYGG | 1 cut(s) | 327 |
| BtsCI | GGATG | 8 cut(s) | 77, 385, 415, 517, 607, 1381, 1423, 1438 |
| BtsIMutI | CAGTG | 2 cut(s) | 1257, 1346 |
| BveI | ACCTGC | 1 cut(s) | 1660 |
| Cac8I | GCNNGC | 4 cut(s) | 1659, 1762, 1819, 1889 |
| CfoI | GCGC | 1 cut(s) | 1760 |
| Cfr10I | RCCGGY | 2 cut(s) | 1873, 2220 |
| Cfr13I | GGNCC | 2 cut(s) | 1634, 1876 |
| Csp6I | GTAC | 5 cut(s) | 1226, 1610, 1744, 1985, 2224 |
| CspAI | ACCGGT | 2 cut(s) | 1873, 2220 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 99, 134 |
| CviAII | CATG | 4 cut(s) | 328, 683, 1613, 1627 |
| CviQI | GTAC | 5 cut(s) | 1226, 1610, 1744, 1985, 2224 |
| DdeI | CTNAG | 5 cut(s) | 57, 707, 1606, 1641, 1936 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1358 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1358 |
| Eam1104I | CTCTTC | 6 cut(s) | 60, 561, 1011, 1062, 1473, 1503 |
| EarI | CTCTTC | 6 cut(s) | 60, 561, 1011, 1062, 1473, 1503 |
| EciI | GGCGGA | 2 cut(s) | 248, 536 |
| Eco130I | CCWWGG | 3 cut(s) | 81, 327, 375 |
| Eco32I | GATATC | 1 cut(s) | 88 |
| Eco47I | GGWCC | 1 cut(s) | 1876 |
| Eco47III | AGCGCT | 1 cut(s) | 1759 |
| Eco57I | CTGAAG | 5 cut(s) | 327, 594, 1158, 1670, 1944 |
| Eco88I | CYCGRG | 1 cut(s) | 734 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1242 |
| EcoRI | GAATTC | 1 cut(s) | 1913 |
| EcoRII | CCWGG | 4 cut(s) | 1582, 1630, 1826, 2158 |
| EcoRV | GATATC | 1 cut(s) | 88 |
| EcoT14I | CCWWGG | 3 cut(s) | 81, 327, 375 |
| ErhI | CCWWGG | 3 cut(s) | 81, 327, 375 |
| FaeI | CATG | 4 cut(s) | 331, 686, 1616, 1630 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1559, 1591 |
| FatI | CATG | 4 cut(s) | 327, 682, 1612, 1626 |
| FauI | CCCGC | 2 cut(s) | 1880, 1890 |
| FauNDI | CATATG | 2 cut(s) | 1413, 2080 |
| FbaI | TGATCA | 2 cut(s) | 583, 2196 |
| FblI | GTMKAC | 1 cut(s) | 1485 |
| Fnu4HI | GCNGC | 4 cut(s) | 129, 1466, 1860, 1932 |
| FokI | GGATG | 8 cut(s) | 64, 392, 422, 524, 614, 1388, 1425, 1430 |
| Fsp4HI | GCNGC | 4 cut(s) | 129, 1466, 1860, 1932 |
| FspBI | CTAG | 3 cut(s) | 1239, 1290, 1761 |
| GlaI | GCGC | 1 cut(s) | 1759 |
| GluI | GCNGC | 4 cut(s) | 129, 1466, 1860, 1932 |
| GsaI | CCCAGC | 1 cut(s) | 1502 |
| GsuI | CTGGAG | 1 cut(s) | 1322 |
| HaeII | RGCGCY | 1 cut(s) | 1761 |
| HaeIII | GGCC | 2 cut(s) | 1146, 1635 |
| HapII | CCGG | 3 cut(s) | 1442, 1874, 2221 |
| HhaI | GCGC | 1 cut(s) | 1760 |
| Hin1I | GRCGYC | 1 cut(s) | 1194 |
| Hin1II | CATG | 4 cut(s) | 331, 686, 1616, 1630 |
| Hin6I | GCGC | 1 cut(s) | 1758 |
| HinP1I | GCGC | 1 cut(s) | 1758 |
| HincII | GTYRAC | 2 cut(s) | 1192, 1306 |
| HindII | GTYRAC | 2 cut(s) | 1192, 1306 |
| HindIII | AAGCTT | 1 cut(s) | 1568 |
| HinfI | GANTC | 5 cut(s) | 299, 614, 763, 1364, 1561 |
| HpaII | CCGG | 3 cut(s) | 1442, 1874, 2221 |
| HphI | GGTGA | 7 cut(s) | 40, 152, 221, 232, 446, 554, 1400 |
| Hpy166II | GTNNAC | 7 cut(s) | 143, 437, 1192, 1306, 1486, 2041, 2175 |
| Hpy188I | TCNGA | 9 cut(s) | 22, 58, 298, 352, 613, 1566, 1939, 1966, 2135 |
| Hpy8I | GTNNAC | 7 cut(s) | 143, 437, 1192, 1306, 1486, 2041, 2175 |
| Hpy99I | CGWCG | 1 cut(s) | 1679 |
| HpyCH4III | ACNGT | 2 cut(s) | 842, 2179 |
| HpyCH4IV | ACGT | 2 cut(s) | 1194, 2166 |
| HpyCH4V | TGCA | 6 cut(s) | 128, 422, 1031, 1214, 1712, 1862 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 428, 554, 1757 |
| HpyF3I | CTNAG | 5 cut(s) | 57, 707, 1606, 1641, 1936 |
| HpySE526I | ACGT | 2 cut(s) | 1194, 2166 |
| Hsp92I | GRCGYC | 1 cut(s) | 1194 |
| Hsp92II | CATG | 4 cut(s) | 331, 686, 1616, 1630 |
| HspAI | GCGC | 1 cut(s) | 1758 |
| KpnI | GGTACC | 1 cut(s) | 1229 |
| Ksp22I | TGATCA | 2 cut(s) | 583, 2196 |
| LmnI | GCTCC | 3 cut(s) | 31, 703, 1648 |
| Lsp1109I | GCAGC | 4 cut(s) | 140, 1452, 1846, 1918 |
| LweI | GCATC | 6 cut(s) | 487, 592, 1300, 1447, 1485, 1918 |
| MaeI | CTAG | 3 cut(s) | 1239, 1290, 1761 |
| MaeII | ACGT | 2 cut(s) | 1194, 2166 |
| MaeIII | GTNAC | 4 cut(s) | 46, 1448, 1831, 2216 |
| MmeI | TCCRAC | 1 cut(s) | 2087 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1971, 2058 |
| MseI | TTAA | 6 cut(s) | 750, 1461, 1578, 1784, 1893, 2006 |
| MslI | CAYNNNNRTG | 3 cut(s) | 351, 1339, 1416 |
| MspA1I | CMGCKG | 1 cut(s) | 305 |
| MspCI | CTTAAG | 1 cut(s) | 1892 |
| MspI | CCGG | 3 cut(s) | 1442, 1874, 2221 |
| MspR9I | CCNGG | 4 cut(s) | 1584, 1632, 1828, 2160 |
| MvaI | CCWGG | 4 cut(s) | 1584, 1632, 1828, 2160 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 428, 554, 1757 |
| NcoI | CCATGG | 1 cut(s) | 327 |
| NdeI | CATATG | 2 cut(s) | 1413, 2080 |
| NheI | GCTAGC | 1 cut(s) | 1760 |
| NlaIII | CATG | 4 cut(s) | 331, 686, 1616, 1630 |
| NlaIV | GGNNCC | 4 cut(s) | 1227, 1402, 1423, 1636 |
| NmuCI | GTSAC | 2 cut(s) | 46, 1831 |
| NspI | RCATGY | 1 cut(s) | 1630 |
| NspV | TTCGAA | 1 cut(s) | 1299 |
| OliI | CACNNNNGTG | 1 cut(s) | 1339 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 1305, 1314 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1971, 2058 |
| PfeI | GAWTC | 5 cut(s) | 299, 614, 763, 1364, 1561 |
| PinAI | ACCGGT | 2 cut(s) | 1873, 2220 |
| PkrI | GCNGC | 4 cut(s) | 130, 1467, 1861, 1933 |
| PsiI | TTATAA | 1 cut(s) | 1529 |
| Psp1406I | AACGTT | 1 cut(s) | 2166 |
| Psp6I | CCWGG | 4 cut(s) | 1582, 1630, 1826, 2158 |
| PspFI | CCCAGC | 1 cut(s) | 1498 |
| PspGI | CCWGG | 4 cut(s) | 1582, 1630, 1826, 2158 |
| PspN4I | GGNNCC | 4 cut(s) | 1227, 1402, 1423, 1636 |
| PspPI | GGNCC | 2 cut(s) | 1634, 1876 |
| PstI | CTGCAG | 1 cut(s) | 1864 |
| PvuII | CAGCTG | 1 cut(s) | 305 |
| RsaI | GTAC | 5 cut(s) | 1227, 1611, 1745, 1986, 2225 |
| RsaNI | GTAC | 5 cut(s) | 1226, 1610, 1744, 1985, 2224 |
| RseI | CAYNNNNRTG | 3 cut(s) | 351, 1339, 1416 |
| SaqAI | TTAA | 6 cut(s) | 750, 1461, 1578, 1784, 1893, 2006 |
| SatI | GCNGC | 4 cut(s) | 129, 1466, 1860, 1932 |
| Sau96I | GGNCC | 2 cut(s) | 1634, 1876 |
| ScrFI | CCNGG | 4 cut(s) | 1584, 1632, 1828, 2160 |
| SfaNI | GCATC | 6 cut(s) | 487, 592, 1300, 1447, 1485, 1918 |
| SfcI | CTRYAG | 2 cut(s) | 1753, 1860 |
| SfuI | TTCGAA | 1 cut(s) | 1299 |
| SinI | GGWCC | 1 cut(s) | 1876 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 351, 1339, 1416 |
| SmlI | CTYRAG | 3 cut(s) | 1619, 1847, 1892 |
| SmoI | CTYRAG | 3 cut(s) | 1619, 1847, 1892 |
| SsiI | CCGC | 6 cut(s) | 233, 521, 548, 557, 1887, 1897 |
| SspMI | CTAG | 3 cut(s) | 1239, 1290, 1761 |
| StyD4I | CCNGG | 4 cut(s) | 1582, 1630, 1826, 2158 |
| StyI | CCWWGG | 3 cut(s) | 81, 327, 375 |
| TaaI | ACNGT | 2 cut(s) | 842, 2179 |
| TaiI | ACGT | 2 cut(s) | 1197, 2169 |
| TaqI | TCGA | 6 cut(s) | 766, 1023, 1299, 1317, 1362, 1367 |
| TatI | WGTACW | 1 cut(s) | 1609 |
| TfiI | GAWTC | 5 cut(s) | 299, 614, 763, 1364, 1561 |
| Tru1I | TTAA | 6 cut(s) | 750, 1461, 1578, 1784, 1893, 2006 |
| Tru9I | TTAA | 6 cut(s) | 750, 1461, 1578, 1784, 1893, 2006 |
| TscAI | CASTG | 2 cut(s) | 1264, 1346 |
| TseFI | GTSAC | 2 cut(s) | 46, 1831 |
| TseI | GCWGC | 4 cut(s) | 128, 1465, 1859, 1931 |
| Tsp45I | GTSAC | 2 cut(s) | 46, 1831 |
| TspDTI | ATGAA | 9 cut(s) | 219, 369, 627, 963, 1491, 1521, 1714, 1843, 2085 |
| TspGWI | ACGGA | 1 cut(s) | 1850 |
| TspRI | CASTG | 2 cut(s) | 1264, 1346 |
| Vha464I | CTTAAG | 1 cut(s) | 1892 |
| VpaK11BI | GGWCC | 1 cut(s) | 1876 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1242 |
| XapI | RAATTY | 6 cut(s) | 634, 967, 1703, 1805, 1913, 2054 |
| XceI | RCATGY | 1 cut(s) | 1630 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1411 |
| XmiI | GTMKAC | 1 cut(s) | 1485 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1971, 2058 |
| XspI | CTAG | 3 cut(s) | 1239, 1290, 1761 |
| ZraI | GACGTC | 1 cut(s) | 1195 |