RLG00000032344

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001644
Physical Location & Seq
Forward (+)
13720994 .. 13722966
1973 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032344

Sequence Viewer

Length: 1692 bp
ATGACTGTGGAGAAGACCCAGGTGGCGGTGGCGGTGGCGGCTGAGGTCGTGGAGGTTGAGGAGGCGGTGAAGGAGGTGAAGGAGTGCGAGAAGGCTGTTGTTGAGGATTTGGTGGAGGAATCCGAGTCCAAGCCAACCACTGTTGAGAAGAGCAATTCTTATAAGGAGGAGAGCAACTATCTCTCTGATCTCAAGGAGTTTGAGAAGAAGGCTTTGGTTGAGCTCAAATCGAAGCTCGAAGAAGCGATTTTGGGCAACAACATTTTTAAGAAAGATGAGCCCAAGAAGGTTGAGGAGAAGGAGAAGGTGGTAGCTGAGGTCGAGGAGAAGAAGGAAGAGAAAACAGAGGCTGCTGTGAAAAAAACAGAGGAAACAGAGCCTGCTGTGAAAGCAGAGGAAACAGAGACTGCTGTGAAAGCAGAGGATGTGAAAACAGAGGAAACAGAGACTGCGCTGAAAGCAGAGGATGTGAAAACAGAGGAAACAGAGACTGCTGTGAAAGCAGAGGAAACAGAGGCTGTTGTGAAAACAGAATGCGCTGAAAAAACAGAGGAAGGAGAAAAGGCACCAGAATTGGCTGAGAAGAAAGAAGAAGTAGCAGTTCCAGTATGCGATGAAGAAGTGAAACCCGAATCCAGAGAAGTTGTCGACACAGATATCTCACTCTGGGGTGTGCCTCTGTTCCCTGGAAAAGGATCTGAGGGAACTGATGTGGTGCTCTTGAAATTCTTGAGGGCTAGGGAGTTCAAGGTGAACGAAGCGTTCGAGATGCTGAAGAAGACTCTGCAGTGGAGGAAGGAGTCCAAAATTGATTCAATTTTGGATGAGGAGATCTGCTCTGATCTGAGCTCTGCTGCTTACTTGAACGGCGTGGATCGCGAAGGCCACCCTGTTTGCTACAACATCTTCGGAGTTTTTGACAACGAAGAGCTTTACCAGAAGACGTTCGGAAATGAAGAGAAGAGAGGCCAATTCTTGAGGTGGAGGGTTCAATTGATGGAGAAGAGTATCCAGAAGCTTGAATTGAGGCCCGGTGGTGCTACTTCTCTGCTGCAGATCAATGACCTCAAGAACTCGCCTGGACCAATCAAGAAGGAGCTTAGGGTTGCAACAAAGCAAGCGGTTGGGCTCTTGCAGGACAATTACCCAGAATTGGTTGCCAAGAATATTTTCATCAATGTTCCATTCTGGTACTATGCCCTCAATGCTCTGTTGTCTCCATTCTTGACTCAACGAACCAAGAGCAAGTTTGTTGTGGCTCGCCCCGCTAAGGTCACCGAGACGCTTCTCAAGTACATTCCAGCTGAGGAGATACCGGTTCATTATGGTGGCTTCAAGAGAGAAAATGACACCGAGTTCTTCTCCTCCGAAGATGGTTCTGTTTCTGAGCTAACTCTCAAGGCCGGATCAACCCAAACCATAGAGATTGATGTAGCTGAGGTTGATAATAGCACATTGGTTTGGGATGTGAGTGTTTTGGGATGGGAAGTGAATTACAAGGAGGAGTTTGTTCCTAGTGATGAAGGGTCATACACCATTATTGTTCAGAAGAAGAAGAAGATCGGAGCTAATGAAGGGCCTATTCGCAACACTTTCCGAAGCAATGAGCCTGGAAAGGTTGTCCTGACAATCGAGAACACTTCGAGCAAGAAGAAGAGGGTGCTGTACCGGCACAAGTCCAACAAGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

564

Amino Acids

63.43

Weight (kDa)

4.91

Isoelectric Point (pI)

51.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_N PF03765 210 - 260 6.3e-09 CRAL/TRIO, N-terminal domain
CRAL_TRIO PF00650 285 - 444 1.3e-23 CRAL/TRIO domain
GOLD_PATL1_C PF25099 467 - 552 1.2e-36 Patellin-1-like, C-terminal GOLD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 162
AccB1I GGYRCC 1 cut(s) 565
AccI GTMKAC 1 cut(s) 648
AccII CGCG 1 cut(s) 879
AciI CCGC 6 cut(s) 26, 32, 38, 65, 1121, 1266
AclWI GGATC 3 cut(s) 703, 882, 1414
AcsI RAATTY 1 cut(s) 725
AcuI CTGAAG 1 cut(s) 794
AfaI GTAC 3 cut(s) 1193, 1295, 1667
AfiI CCNNNNNNNGG 4 cut(s) 25, 692, 1153, 1270
AgeI ACCGGT 1 cut(s) 1315
AgsI TTSAA 7 cut(s) 724, 748, 816, 865, 992, 1022, 1336
AjnI CCWGG 4 cut(s) 18, 685, 1078, 1609
AjuI GAANNNNNNNTTGG 4 cut(s) 197, 229, 1154, 1186
Alw21I GWGCWC 3 cut(s) 225, 720, 851
Alw26I GTCTC 5 cut(s) 398, 440, 482, 1221, 1274
AlwI GGATC 3 cut(s) 703, 882, 1414
AlwNI CAGNNNCTG 6 cut(s) 350, 380, 407, 449, 491, 518
AoxI GGCC 5 cut(s) 883, 967, 1028, 1401, 1577
ApeKI GCWGC 3 cut(s) 350, 854, 1051
ApoI RAATTY 1 cut(s) 725
AsiGI ACCGGT 1 cut(s) 1315
Asp700I GAANNNNTTC 2 cut(s) 944, 1169
AspLEI GCGC 2 cut(s) 454, 539
AspS9I GGNCC 3 cut(s) 1029, 1082, 1577
AsuC2I CCSGG 1 cut(s) 1032
AsuHPI GGTGA 4 cut(s) 79, 88, 763, 1267
AvaII GGWCC 1 cut(s) 1082
BanI GGYRCC 1 cut(s) 565
BanII GRGCYC 4 cut(s) 225, 282, 851, 1131
BbsI GAAGAC 3 cut(s) 20, 785, 947
Bbv12I GWGCWC 3 cut(s) 225, 720, 851
BbvCI CCTCAGC 4 cut(s) 42, 315, 1305, 1437
BbvI GCAGC 3 cut(s) 337, 841, 1038
BccI CCATC 3 cut(s) 991, 1367, 1476
BceAI ACGGC 1 cut(s) 883
BciT130I CCWGG 4 cut(s) 20, 687, 1080, 1611
BciVI GTATCC 1 cut(s) 1019
BcnI CCSGG 1 cut(s) 1032
BcoDI GTCTC 5 cut(s) 398, 440, 482, 1221, 1274
BfaI CTAG 2 cut(s) 738, 1515
BfmI CTRYAG 2 cut(s) 785, 1052
BfuI GTATCC 1 cut(s) 1019
BglII AGATCT 1 cut(s) 831
BisI GCNGC 4 cut(s) 39, 351, 855, 1052
BlsI GCNGC 4 cut(s) 40, 352, 856, 1053
Bme1390I CCNGG 5 cut(s) 20, 687, 1032, 1080, 1611
Bme18I GGWCC 1 cut(s) 1082
BmgT120I GGNCC 3 cut(s) 1029, 1082, 1577
BmiI GGNNCC 1 cut(s) 567
BmrFI CCNGG 5 cut(s) 20, 687, 1032, 1080, 1611
BmsI GCATC 1 cut(s) 759
BpiI GAAGAC 3 cut(s) 20, 785, 947
BplI GAGNNNNNCTC 4 cut(s) 821, 853, 1346, 1378
Bpu10I CCTNAGC 6 cut(s) 42, 315, 1100, 1269, 1305, 1437
BpuEI CTTGAG 6 cut(s) 176, 751, 997, 1052, 1274, 1382
BpuMI CCSGG 1 cut(s) 1032
BsaJI CCNNGG 2 cut(s) 18, 685
BsaWI WCCGGW 1 cut(s) 1315
Bsc4I CCNNNNNNNGG 4 cut(s) 25, 692, 1153, 1270
Bse118I RCCGGY 2 cut(s) 1315, 1668
Bse1I ACTGG 1 cut(s) 605
Bse3DI GCAATG 1 cut(s) 1609
BseBI CCWGG 4 cut(s) 20, 687, 1080, 1611
BseDI CCNNGG 2 cut(s) 18, 685
BseGI GGATG 5 cut(s) 430, 472, 829, 1471, 1487
BseLI CCNNNNNNNGG 4 cut(s) 25, 692, 1153, 1270
BseMI GCAATG 1 cut(s) 1609
BseMII CTCAG 8 cut(s) 33, 306, 570, 690, 836, 1296, 1377, 1428
BseNI ACTGG 1 cut(s) 605
BseRI GAGGAG 8 cut(s) 74, 182, 308, 338, 842, 1322, 1354, 1517
BseXI GCAGC 3 cut(s) 337, 841, 1038
Bsh1236I CGCG 1 cut(s) 879
BshFI GGCC 5 cut(s) 885, 969, 1030, 1403, 1579
BshNI GGYRCC 1 cut(s) 565
BshTI ACCGGT 1 cut(s) 1315
BsiHKAI GWGCWC 3 cut(s) 225, 720, 851
BsiSI CCGG 4 cut(s) 1032, 1316, 1404, 1669
BslI CCNNNNNNNGG 4 cut(s) 25, 692, 1153, 1270
BsmAI GTCTC 5 cut(s) 398, 440, 482, 1221, 1274
BsmBI CGTCTC 1 cut(s) 1274
BsmI GAATGC 1 cut(s) 539
BsnI GGCC 5 cut(s) 885, 969, 1030, 1403, 1579
Bsp1286I GDGCHC 5 cut(s) 225, 282, 720, 851, 1131
Bsp143I GATC 8 cut(s) 187, 695, 831, 841, 874, 1056, 1406, 1560
Bsp68I TCGCGA 1 cut(s) 879
BspACI CCGC 6 cut(s) 26, 32, 38, 65, 1121, 1266
BspANI GGCC 5 cut(s) 885, 969, 1030, 1403, 1579
BspCNI CTCAG 8 cut(s) 34, 307, 571, 691, 837, 1297, 1378, 1429
BspFNI CGCG 1 cut(s) 879
BspLI GGNNCC 1 cut(s) 567
BspMAI CTGCAG 2 cut(s) 789, 1056
BspPI GGATC 3 cut(s) 703, 882, 1414
BspQI GCTCTTC 2 cut(s) 143, 921
BspT107I GGYRCC 1 cut(s) 565
BsrDI GCAATG 1 cut(s) 1609
BsrFI RCCGGY 2 cut(s) 1315, 1668
BsrI ACTGG 1 cut(s) 605
BssAI RCCGGY 2 cut(s) 1315, 1668
BssECI CCNNGG 2 cut(s) 18, 685
BssMI GATC 8 cut(s) 187, 695, 831, 841, 874, 1056, 1406, 1560
Bst2UI CCWGG 4 cut(s) 20, 687, 1080, 1611
Bst4CI ACNGT 2 cut(s) 7, 142
Bst6I CTCTTC 7 cut(s) 143, 330, 921, 951, 956, 998, 1649
BstC8I GCNNGC 3 cut(s) 381, 1119, 1261
BstEII GGTNACC 1 cut(s) 1273
BstENI CCTNNNNNAGG 1 cut(s) 690
BstF5I GGATG 5 cut(s) 430, 472, 829, 1471, 1487
BstFNI CGCG 1 cut(s) 879
BstHHI GCGC 2 cut(s) 454, 539
BstKTI GATC 8 cut(s) 190, 698, 834, 844, 877, 1059, 1409, 1563
BstMAI GTCTC 5 cut(s) 398, 440, 482, 1221, 1274
BstMBI GATC 8 cut(s) 187, 695, 831, 841, 874, 1056, 1406, 1560
BstMWI GCNNNNNNNGC 9 cut(s) 38, 389, 416, 458, 500, 876, 1205, 1265, 1669
BstNI CCWGG 4 cut(s) 20, 687, 1080, 1611
BstPI GGTNACC 1 cut(s) 1273
BstSCI CCNGG 5 cut(s) 18, 685, 1030, 1078, 1609
BstSFI CTRYAG 2 cut(s) 785, 1052
BstUI CGCG 1 cut(s) 879
BstV1I GCAGC 3 cut(s) 337, 841, 1038
BstV2I GAAGAC 3 cut(s) 20, 785, 947
BstX2I RGATCY 2 cut(s) 695, 831
BstYI RGATCY 2 cut(s) 695, 831
BsuI GTATCC 1 cut(s) 1019
BsuRI GGCC 5 cut(s) 885, 969, 1030, 1403, 1579
BtgZI GCGATG 1 cut(s) 627
BtsCI GGATG 5 cut(s) 430, 472, 829, 1471, 1487
BtsI GCAGTG 1 cut(s) 794
BtsIMutI CAGTG 2 cut(s) 138, 794
BtuMI TCGCGA 1 cut(s) 879
Cac8I GCNNGC 3 cut(s) 381, 1119, 1261
CaiI CAGNNNCTG 6 cut(s) 350, 380, 407, 449, 491, 518
CfoI GCGC 2 cut(s) 454, 539
Cfr10I RCCGGY 2 cut(s) 1315, 1668
Cfr13I GGNCC 3 cut(s) 1029, 1082, 1577
CseI GACGC 1 cut(s) 1291
Csp6I GTAC 3 cut(s) 1192, 1294, 1666
CspAI ACCGGT 1 cut(s) 1315
CspCI CAANNNNNGTGG 2 cut(s) 875, 910
CviQI GTAC 3 cut(s) 1192, 1294, 1666
DpnI GATC 8 cut(s) 189, 697, 833, 843, 876, 1058, 1408, 1562
DpnII GATC 8 cut(s) 187, 695, 831, 841, 874, 1056, 1406, 1560
Eam1104I CTCTTC 7 cut(s) 143, 330, 921, 951, 956, 998, 1649
EarI CTCTTC 7 cut(s) 143, 330, 921, 951, 956, 998, 1649
Ecl136II GAGCTC 2 cut(s) 223, 849
Eco24I GRGCYC 4 cut(s) 225, 282, 851, 1131
Eco32I GATATC 1 cut(s) 658
Eco47I GGWCC 1 cut(s) 1082
Eco53kI GAGCTC 2 cut(s) 223, 849
Eco57I CTGAAG 1 cut(s) 794
Eco91I GGTNACC 1 cut(s) 1273
EcoICRI GAGCTC 2 cut(s) 223, 849
EcoNI CCTNNNNNAGG 1 cut(s) 690
EcoO109I RGGNCCY 1 cut(s) 1577
EcoO65I GGTNACC 1 cut(s) 1273
EcoRII CCWGG 4 cut(s) 18, 685, 1078, 1609
EcoRV GATATC 1 cut(s) 658
EcoT38I GRGCYC 4 cut(s) 225, 282, 851, 1131
Esp3I CGTCTC 1 cut(s) 1274
FaiI YATR 6 cut(s) 162, 610, 1197, 1326, 1421, 1531
FauI CCCGC 1 cut(s) 1273
FblI GTMKAC 1 cut(s) 648
Fnu4HI GCNGC 4 cut(s) 39, 351, 855, 1052
FokI GGATG 5 cut(s) 437, 479, 836, 1478, 1494
FriOI GRGCYC 4 cut(s) 225, 282, 851, 1131
Fsp4HI GCNGC 4 cut(s) 39, 351, 855, 1052
FspBI CTAG 2 cut(s) 738, 1515
GlaI GCGC 2 cut(s) 453, 538
GluI GCNGC 4 cut(s) 39, 351, 855, 1052
HaeIII GGCC 5 cut(s) 885, 969, 1030, 1403, 1579
HapII CCGG 4 cut(s) 1032, 1316, 1404, 1669
HgaI GACGC 1 cut(s) 1291
HhaI GCGC 2 cut(s) 454, 539
Hin6I GCGC 2 cut(s) 452, 537
HinP1I GCGC 2 cut(s) 452, 537
HincII GTYRAC 1 cut(s) 649
HindII GTYRAC 1 cut(s) 649
HindIII AAGCTT 1 cut(s) 1016
HinfI GANTC 7 cut(s) 119, 125, 632, 781, 800, 812, 1228
HpaII CCGG 4 cut(s) 1032, 1316, 1404, 1669
HphI GGTGA 4 cut(s) 79, 88, 763, 1267
Hpy166II GTNNAC 2 cut(s) 649, 754
Hpy8I GTNNAC 2 cut(s) 649, 754
HpyCH4III ACNGT 2 cut(s) 7, 142
HpyCH4IV ACGT 1 cut(s) 944
HpyCH4V TGCA 4 cut(s) 787, 1054, 1109, 1135
HpyF10VI GCNNNNNNNGC 9 cut(s) 38, 389, 416, 458, 500, 876, 1205, 1265, 1669
HpySE526I ACGT 1 cut(s) 944
HspAI GCGC 2 cut(s) 452, 537
Kzo9I GATC 8 cut(s) 187, 695, 831, 841, 874, 1056, 1406, 1560
LguI GCTCTTC 2 cut(s) 143, 921
LmnI GCTCC 2 cut(s) 1096, 1565
Lsp1109I GCAGC 3 cut(s) 337, 841, 1038
LweI GCATC 1 cut(s) 759
MaeI CTAG 2 cut(s) 738, 1515
MaeII ACGT 1 cut(s) 944
MaeIII GTNAC 1 cut(s) 1273
MalI GATC 8 cut(s) 189, 697, 833, 843, 876, 1058, 1408, 1562
MboI GATC 8 cut(s) 187, 695, 831, 841, 874, 1056, 1406, 1560
MfeI CAATTG 1 cut(s) 992
MflI RGATCY 2 cut(s) 695, 831
MhlI GDGCHC 5 cut(s) 225, 282, 720, 851, 1131
MlyI GAGTC 4 cut(s) 134, 775, 809, 1222
MroXI GAANNNNTTC 2 cut(s) 944, 1169
MseI TTAA 1 cut(s) 267
MslI CAYNNNNRTG 1 cut(s) 1326
MspA1I CMGCKG 1 cut(s) 1304
MspI CCGG 4 cut(s) 1032, 1316, 1404, 1669
MspR9I CCNGG 5 cut(s) 20, 687, 1032, 1080, 1611
MunI CAATTG 1 cut(s) 992
Mva1269I GAATGC 1 cut(s) 539
MvaI CCWGG 4 cut(s) 20, 687, 1080, 1611
MvnI CGCG 1 cut(s) 879
MwoI GCNNNNNNNGC 9 cut(s) 38, 389, 416, 458, 500, 876, 1205, 1265, 1669
NciI CCSGG 1 cut(s) 1032
NdeII GATC 8 cut(s) 187, 695, 831, 841, 874, 1056, 1406, 1560
NlaIV GGNNCC 1 cut(s) 567
NmuCI GTSAC 1 cut(s) 1273
NruI TCGCGA 1 cut(s) 879
PciSI GCTCTTC 2 cut(s) 143, 921
PcsI WCGNNNNNNNCGW 1 cut(s) 762
PctI GAATGC 1 cut(s) 539
PdmI GAANNNNTTC 2 cut(s) 944, 1169
PfeI GAWTC 3 cut(s) 119, 632, 812
PinAI ACCGGT 1 cut(s) 1315
PkrI GCNGC 4 cut(s) 40, 352, 856, 1053
PleI GAGTC 4 cut(s) 133, 775, 808, 1222
PpsI GAGTC 4 cut(s) 133, 775, 808, 1222
PsiI TTATAA 1 cut(s) 162
Psp124BI GAGCTC 2 cut(s) 225, 851
Psp6I CCWGG 4 cut(s) 18, 685, 1078, 1609
PspEI GGTNACC 1 cut(s) 1273
PspGI CCWGG 4 cut(s) 18, 685, 1078, 1609
PspN4I GGNNCC 1 cut(s) 567
PspPI GGNCC 3 cut(s) 1029, 1082, 1577
PstI CTGCAG 2 cut(s) 789, 1056
PstNI CAGNNNCTG 6 cut(s) 350, 380, 407, 449, 491, 518
PsuI RGATCY 2 cut(s) 695, 831
PvuII CAGCTG 1 cut(s) 1304
RruI TCGCGA 1 cut(s) 879
RsaI GTAC 3 cut(s) 1193, 1295, 1667
RsaNI GTAC 3 cut(s) 1192, 1294, 1666
RseI CAYNNNNRTG 1 cut(s) 1326
SacI GAGCTC 2 cut(s) 225, 851
SalI GTCGAC 1 cut(s) 647
SapI GCTCTTC 2 cut(s) 143, 921
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 4 cut(s) 39, 351, 855, 1052
Sau3AI GATC 8 cut(s) 187, 695, 831, 841, 874, 1056, 1406, 1560
Sau96I GGNCC 3 cut(s) 1029, 1082, 1577
SchI GAGTC 4 cut(s) 134, 775, 809, 1222
ScrFI CCNGG 5 cut(s) 20, 687, 1032, 1080, 1611
SduI GDGCHC 5 cut(s) 225, 282, 720, 851, 1131
SfaNI GCATC 1 cut(s) 759
SfcI CTRYAG 2 cut(s) 785, 1052
SinI GGWCC 1 cut(s) 1082
SmiMI CAYNNNNRTG 1 cut(s) 1326
SmlI CTYRAG 6 cut(s) 191, 730, 976, 1067, 1289, 1397
SmoI CTYRAG 6 cut(s) 191, 730, 976, 1067, 1289, 1397
SsiI CCGC 6 cut(s) 26, 32, 38, 65, 1121, 1266
SspI AATATT 1 cut(s) 1168
SspMI CTAG 2 cut(s) 738, 1515
SstI GAGCTC 2 cut(s) 225, 851
StyD4I CCNGG 5 cut(s) 18, 685, 1030, 1078, 1609
TaaI ACNGT 2 cut(s) 7, 142
TaiI ACGT 1 cut(s) 947
TaqI TCGA 7 cut(s) 230, 237, 321, 648, 765, 1632, 1643
TatI WGTACW 1 cut(s) 1293
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 3 cut(s) 119, 632, 812
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 2 cut(s) 145, 794
TseFI GTSAC 1 cut(s) 1273
TseI GCWGC 3 cut(s) 350, 854, 1051
Tsp45I GTSAC 1 cut(s) 1273
TspDTI ATGAA 6 cut(s) 630, 969, 1162, 1310, 1536, 1587
TspRI CASTG 2 cut(s) 145, 794
VpaK11BI GGWCC 1 cut(s) 1082
XagI CCTNNNNNAGG 1 cut(s) 690
XapI RAATTY 1 cut(s) 725
XmiI GTMKAC 1 cut(s) 648
XmnI GAANNNNTTC 2 cut(s) 944, 1169
XspI CTAG 2 cut(s) 738, 1515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.