Rw5G011700

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001644
Physical Location & Seq
Forward (+)
13480421 .. 13482653
2233 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G011700.1

Sequence Viewer

Length: 1539 bp
ATGACTGTGGAGGAGACCCAGGTGGCGGTGGCGGCGGCGGCTGAGGTCGTGGAGGTTGAGGAGCCGGTGAAGGAGGTGAAGGAGTGCGAGAAGGCTGTTGTTGAGGAATCCGAGTCCAAGCCAACCACTGTTGAGAAGAGCACTTCTTATAAGGAGGAGAGCAACTATCTCTCTGATCTCAAGGAGTTTGAGAAGAAGGCTTTGGTTGAGCTCAAATCGAAGCTCGAAGAAGCCATTTTGGGCAACAACATTTTCAAGAAAGATGAGCCCAAGAAGGTTGAGGAGAAGGTGAAGGCGGTAGCTGAGGTCGAGGACAAGAAGGAAGAGAAAACAGAGGCTGCTGTGAAAAAAACAGAGGAAACAGAGGCTGCTGTGAAAACAGAGTGCTCTGAAAAAACAGAGGAAGGAGAAAAGGCACCAGAATTGACTGAGAAGAAAGAAGAAGTAGCAGTATGCGATGAAGAAGTGAAACTCGAATCCAGCGAAGTCGTCGACACAGATATCTCACTCTGGGGTGTGCCTCTGTTCCCTGGAAAAGGATCTGAGGGAACTGATGTGGTGCTCTTGAAATTCTTGAGGGCCAGGGAGTTCAAGGTGAACGAAGCCTTCGAGATGCTGAAGAAGACTCTGCAGTGGAGGAAGGAGTCGAAAATTGATTCAATTTTGGATGAGGAGATCTGCTCTGATCTGAGCTCTGCTGCTTACTTGAACGGCGTGGATCGCGAAGGCCACCCTGTTTGCTACAACATCTTCGGAGTCTTTGACAACGAAGAGCTTTACCAGAAGACATTCGGAAATGAAGAGAAGAGAGGCCAATTCTTGAGGTGGAGGGTTCAATTGATGGAGAAGAGTATCCAGAAGCTTGAATTAAGGCCTGGTGGTGCTACTTCTCTGCTTCAGATAAATGACCTCAAGAACTCGCCTGGACCAATCAAGAAGGAGCTTAGGGTTGCAACAAAGCAAGCTGTTGGGCTCTTGCAGGACAATTACCCTGAATTGGTTGCCAAAAATATTTTCATCAATGTTCCATTCTGGTACTATGCACTCAATGCTCTGTTGTCTCCATTCTTGACTCAAAGAACCAAGAGCAAGTTTGTGGTGGCTCGCCCCGCTAAGGTCACCGAGACCCTTCTCAAGTACATTCCAGCTGAGGAGATACCGGTTCAGTATGGTGGCTTCAAGAGGGAAAATGACACCGAGTTCTTCTCCTCCGAAGATGGTTCTGTTTCTGAGCTAACTCTCAAGGCCGGATCAACCCAAACCATAGAGATTGATGTAGCTGAGGTTGATAATAGCACATTGGTTTGGGATGTGAGTGTTTTGGGATGGGAAGTGAATTACAAGGAGGAGTTTGTTCCTAGTGATGAAGGGTCATACACCATTATTGTTCAGAAGAAGAAGAAGATCGGAGCTCATGAAGGGCCTATTCGCAACACTTTCCGAAGCAATGAGCCTGGAAAGGTTGTCCTGACAATCGAGAACACTTCGAGCAAGAAGAAGAGGGTGCTGTACCGGCACAAGTCCAACAAGTGCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

512

Amino Acids

57.87

Weight (kDa)

5.07

Isoelectric Point (pI)

48.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_N PF03765 148 - 208 1.9e-08 CRAL/TRIO, N-terminal domain
CRAL_TRIO PF00650 233 - 392 6.4e-24 CRAL/TRIO domain
GOLD_PATL1_C PF25099 415 - 500 1.3e-36 Patellin-1-like, C-terminal GOLD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 150
AccB1I GGYRCC 1 cut(s) 415
AccI GTMKAC 1 cut(s) 492
AccII CGCG 1 cut(s) 723
AciI CCGC 6 cut(s) 26, 32, 35, 38, 296, 1110
AclWI GGATC 3 cut(s) 547, 726, 1258
AcsI RAATTY 1 cut(s) 569
AcuI CTGAAG 2 cut(s) 638, 881
AfaI GTAC 3 cut(s) 1037, 1139, 1511
AfiI CCNNNNNNNGG 4 cut(s) 25, 536, 997, 1114
AgeI ACCGGT 1 cut(s) 1159
AgsI TTSAA 8 cut(s) 256, 568, 592, 660, 709, 836, 866, 1180
AjnI CCWGG 6 cut(s) 18, 529, 581, 874, 922, 1453
AjuI GAANNNNNNNTTGG 4 cut(s) 185, 217, 998, 1030
Alw21I GWGCWC 6 cut(s) 143, 213, 389, 564, 695, 1414
Alw26I GTCTC 3 cut(s) 8, 1065, 1118
AlwI GGATC 3 cut(s) 547, 726, 1258
AlwNI CAGNNNCTG 2 cut(s) 338, 368
AoxI GGCC 6 cut(s) 579, 727, 811, 872, 1245, 1421
ApeKI GCWGC 3 cut(s) 338, 368, 698
ApoI RAATTY 1 cut(s) 569
AsiGI ACCGGT 1 cut(s) 1159
Asp700I GAANNNNTTC 1 cut(s) 788
AspS9I GGNCC 3 cut(s) 579, 926, 1421
AsuHPI GGTGA 5 cut(s) 79, 88, 301, 607, 1111
AvaII GGWCC 1 cut(s) 926
BanI GGYRCC 1 cut(s) 415
BanII GRGCYC 5 cut(s) 213, 270, 695, 975, 1414
BarI GAAGNNNNNNTAC 2 cut(s) 1160, 1192
BbsI GAAGAC 2 cut(s) 629, 791
Bbv12I GWGCWC 6 cut(s) 143, 213, 389, 564, 695, 1414
BbvCI CCTCAGC 4 cut(s) 42, 303, 1149, 1281
BbvI GCAGC 3 cut(s) 325, 355, 685
BccI CCATC 3 cut(s) 835, 1211, 1320
BceAI ACGGC 1 cut(s) 727
BciT130I CCWGG 6 cut(s) 20, 531, 583, 876, 924, 1455
BciVI GTATCC 1 cut(s) 863
BcoDI GTCTC 3 cut(s) 8, 1065, 1118
BfaI CTAG 1 cut(s) 1359
BfmI CTRYAG 1 cut(s) 629
BfuI GTATCC 1 cut(s) 863
BglII AGATCT 1 cut(s) 675
BisI GCNGC 6 cut(s) 33, 36, 39, 339, 369, 699
BlsI GCNGC 6 cut(s) 34, 37, 40, 340, 370, 700
Bme1390I CCNGG 6 cut(s) 20, 531, 583, 876, 924, 1455
Bme18I GGWCC 1 cut(s) 926
BmgT120I GGNCC 3 cut(s) 579, 926, 1421
BmiI GGNNCC 2 cut(s) 63, 417
BmrFI CCNGG 6 cut(s) 20, 531, 583, 876, 924, 1455
BmsI GCATC 1 cut(s) 603
BpiI GAAGAC 2 cut(s) 629, 791
BplI GAGNNNNNCTC 4 cut(s) 665, 697, 1190, 1222
Bpu10I CCTNAGC 6 cut(s) 42, 303, 944, 1113, 1149, 1281
BpuEI CTTGAG 6 cut(s) 164, 595, 841, 896, 1118, 1226
BsaI GGTCTC 2 cut(s) 8, 1118
BsaJI CCNNGG 3 cut(s) 18, 529, 582
BsaWI WCCGGW 1 cut(s) 1159
Bsc4I CCNNNNNNNGG 4 cut(s) 25, 536, 997, 1114
Bse118I RCCGGY 3 cut(s) 64, 1159, 1512
Bse3DI GCAATG 1 cut(s) 1453
BseBI CCWGG 6 cut(s) 20, 531, 583, 876, 924, 1455
BseDI CCNNGG 3 cut(s) 18, 529, 582
BseGI GGATG 3 cut(s) 673, 1315, 1331
BseLI CCNNNNNNNGG 4 cut(s) 25, 536, 997, 1114
BseMI GCAATG 1 cut(s) 1453
BseMII CTCAG 8 cut(s) 33, 294, 420, 534, 680, 1140, 1221, 1272
BseRI GAGGAG 8 cut(s) 26, 74, 170, 296, 686, 1166, 1198, 1361
BseXI GCAGC 3 cut(s) 325, 355, 685
Bsh1236I CGCG 1 cut(s) 723
BshFI GGCC 6 cut(s) 581, 729, 813, 874, 1247, 1423
BshNI GGYRCC 1 cut(s) 415
BshTI ACCGGT 1 cut(s) 1159
BsiHKAI GWGCWC 6 cut(s) 143, 213, 389, 564, 695, 1414
BsiSI CCGG 4 cut(s) 65, 1160, 1248, 1513
BslI CCNNNNNNNGG 4 cut(s) 25, 536, 997, 1114
BsmAI GTCTC 3 cut(s) 8, 1065, 1118
BsnI GGCC 6 cut(s) 581, 729, 813, 874, 1247, 1423
Bso31I GGTCTC 2 cut(s) 8, 1118
Bsp1286I GDGCHC 8 cut(s) 143, 213, 270, 389, 564, 695, 975, 1414
Bsp143I GATC 7 cut(s) 175, 539, 675, 685, 718, 1250, 1404
Bsp68I TCGCGA 1 cut(s) 723
BspACI CCGC 6 cut(s) 26, 32, 35, 38, 296, 1110
BspANI GGCC 6 cut(s) 581, 729, 813, 874, 1247, 1423
BspCNI CTCAG 8 cut(s) 34, 295, 421, 535, 681, 1141, 1222, 1273
BspFNI CGCG 1 cut(s) 723
BspHI TCATGA 1 cut(s) 1414
BspLI GGNNCC 2 cut(s) 63, 417
BspMAI CTGCAG 1 cut(s) 633
BspPI GGATC 3 cut(s) 547, 726, 1258
BspQI GCTCTTC 2 cut(s) 131, 765
BspT107I GGYRCC 1 cut(s) 415
BspTNI GGTCTC 2 cut(s) 8, 1118
BsrDI GCAATG 1 cut(s) 1453
BsrFI RCCGGY 3 cut(s) 64, 1159, 1512
BssAI RCCGGY 3 cut(s) 64, 1159, 1512
BssECI CCNNGG 3 cut(s) 18, 529, 582
BssMI GATC 7 cut(s) 175, 539, 675, 685, 718, 1250, 1404
Bst2UI CCWGG 6 cut(s) 20, 531, 583, 876, 924, 1455
Bst4CI ACNGT 2 cut(s) 7, 130
Bst6I CTCTTC 7 cut(s) 131, 318, 765, 795, 800, 842, 1493
BstAPI GCANNNNNTGC 1 cut(s) 1049
BstC8I GCNNGC 2 cut(s) 963, 1105
BstEII GGTNACC 1 cut(s) 1117
BstENI CCTNNNNNAGG 1 cut(s) 534
BstF5I GGATG 3 cut(s) 673, 1315, 1331
BstFNI CGCG 1 cut(s) 723
BstKTI GATC 7 cut(s) 178, 542, 678, 688, 721, 1253, 1407
BstMAI GTCTC 3 cut(s) 8, 1065, 1118
BstMBI GATC 7 cut(s) 175, 539, 675, 685, 718, 1250, 1404
BstMWI GCNNNNNNNGC 6 cut(s) 32, 38, 720, 1049, 1109, 1513
BstNI CCWGG 6 cut(s) 20, 531, 583, 876, 924, 1455
BstPI GGTNACC 1 cut(s) 1117
BstSCI CCNGG 6 cut(s) 18, 529, 581, 874, 922, 1453
BstSFI CTRYAG 1 cut(s) 629
BstUI CGCG 1 cut(s) 723
BstV1I GCAGC 3 cut(s) 325, 355, 685
BstV2I GAAGAC 2 cut(s) 629, 791
BstX2I RGATCY 2 cut(s) 539, 675
BstYI RGATCY 2 cut(s) 539, 675
BsuI GTATCC 1 cut(s) 863
BsuRI GGCC 6 cut(s) 581, 729, 813, 874, 1247, 1423
BtgZI GCGATG 1 cut(s) 471
BtsCI GGATG 3 cut(s) 673, 1315, 1331
BtsI GCAGTG 1 cut(s) 638
BtsIMutI CAGTG 2 cut(s) 126, 638
BtuMI TCGCGA 1 cut(s) 723
Cac8I GCNNGC 2 cut(s) 963, 1105
CaiI CAGNNNCTG 2 cut(s) 338, 368
CciI TCATGA 1 cut(s) 1414
Cfr10I RCCGGY 3 cut(s) 64, 1159, 1512
Cfr13I GGNCC 3 cut(s) 579, 926, 1421
Csp6I GTAC 3 cut(s) 1036, 1138, 1510
CspAI ACCGGT 1 cut(s) 1159
CspCI CAANNNNNGTGG 2 cut(s) 719, 754
CviAII CATG 1 cut(s) 1415
CviQI GTAC 3 cut(s) 1036, 1138, 1510
DpnI GATC 7 cut(s) 177, 541, 677, 687, 720, 1252, 1406
DpnII GATC 7 cut(s) 175, 539, 675, 685, 718, 1250, 1404
Eam1104I CTCTTC 7 cut(s) 131, 318, 765, 795, 800, 842, 1493
EarI CTCTTC 7 cut(s) 131, 318, 765, 795, 800, 842, 1493
Ecl136II GAGCTC 3 cut(s) 211, 693, 1412
Eco147I AGGCCT 1 cut(s) 874
Eco24I GRGCYC 5 cut(s) 213, 270, 695, 975, 1414
Eco31I GGTCTC 2 cut(s) 8, 1118
Eco32I GATATC 1 cut(s) 502
Eco47I GGWCC 1 cut(s) 926
Eco53kI GAGCTC 3 cut(s) 211, 693, 1412
Eco57I CTGAAG 2 cut(s) 638, 881
Eco91I GGTNACC 1 cut(s) 1117
EcoICRI GAGCTC 3 cut(s) 211, 693, 1412
EcoNI CCTNNNNNAGG 1 cut(s) 534
EcoO109I RGGNCCY 1 cut(s) 1421
EcoO65I GGTNACC 1 cut(s) 1117
EcoRII CCWGG 6 cut(s) 18, 529, 581, 874, 922, 1453
EcoRV GATATC 1 cut(s) 502
EcoT38I GRGCYC 5 cut(s) 213, 270, 695, 975, 1414
FaeI CATG 1 cut(s) 1418
FaiI YATR 7 cut(s) 150, 454, 1041, 1170, 1265, 1375, 1416
FatI CATG 1 cut(s) 1414
FauI CCCGC 1 cut(s) 1117
FblI GTMKAC 1 cut(s) 492
Fnu4HI GCNGC 6 cut(s) 33, 36, 39, 339, 369, 699
FokI GGATG 3 cut(s) 680, 1322, 1338
FriOI GRGCYC 5 cut(s) 213, 270, 695, 975, 1414
Fsp4HI GCNGC 6 cut(s) 33, 36, 39, 339, 369, 699
FspBI CTAG 1 cut(s) 1359
GluI GCNGC 6 cut(s) 33, 36, 39, 339, 369, 699
HaeIII GGCC 6 cut(s) 581, 729, 813, 874, 1247, 1423
HapII CCGG 4 cut(s) 65, 1160, 1248, 1513
Hin1II CATG 1 cut(s) 1418
HincII GTYRAC 1 cut(s) 493
HindII GTYRAC 1 cut(s) 493
HindIII AAGCTT 1 cut(s) 860
HinfI GANTC 8 cut(s) 107, 113, 476, 625, 644, 656, 756, 1072
HpaII CCGG 4 cut(s) 65, 1160, 1248, 1513
HphI GGTGA 5 cut(s) 79, 88, 301, 607, 1111
Hpy166II GTNNAC 2 cut(s) 493, 598
Hpy8I GTNNAC 2 cut(s) 493, 598
Hpy99I CGWCG 1 cut(s) 494
HpyCH4III ACNGT 2 cut(s) 7, 130
HpyCH4V TGCA 4 cut(s) 631, 953, 979, 1043
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 38, 720, 1049, 1109, 1513
Hsp92II CATG 1 cut(s) 1418
Kzo9I GATC 7 cut(s) 175, 539, 675, 685, 718, 1250, 1404
LguI GCTCTTC 2 cut(s) 131, 765
LmnI GCTCC 3 cut(s) 61, 940, 1409
Lsp1109I GCAGC 3 cut(s) 325, 355, 685
LweI GCATC 1 cut(s) 603
MaeI CTAG 1 cut(s) 1359
MaeIII GTNAC 1 cut(s) 1117
MalI GATC 7 cut(s) 177, 541, 677, 687, 720, 1252, 1406
MboI GATC 7 cut(s) 175, 539, 675, 685, 718, 1250, 1404
MfeI CAATTG 1 cut(s) 836
MflI RGATCY 2 cut(s) 539, 675
MhlI GDGCHC 8 cut(s) 143, 213, 270, 389, 564, 695, 975, 1414
MlyI GAGTC 5 cut(s) 122, 619, 653, 765, 1066
MroXI GAANNNNTTC 1 cut(s) 788
MseI TTAA 1 cut(s) 869
MspA1I CMGCKG 1 cut(s) 1148
MspI CCGG 4 cut(s) 65, 1160, 1248, 1513
MspR9I CCNGG 6 cut(s) 20, 531, 583, 876, 924, 1455
MunI CAATTG 1 cut(s) 836
MvaI CCWGG 6 cut(s) 20, 531, 583, 876, 924, 1455
MvnI CGCG 1 cut(s) 723
MwoI GCNNNNNNNGC 6 cut(s) 32, 38, 720, 1049, 1109, 1513
NdeII GATC 7 cut(s) 175, 539, 675, 685, 718, 1250, 1404
NlaIII CATG 1 cut(s) 1418
NlaIV GGNNCC 2 cut(s) 63, 417
NmuCI GTSAC 1 cut(s) 1117
NruI TCGCGA 1 cut(s) 723
PagI TCATGA 1 cut(s) 1414
PceI AGGCCT 1 cut(s) 874
PciSI GCTCTTC 2 cut(s) 131, 765
PcsI WCGNNNNNNNCGW 2 cut(s) 480, 606
PdmI GAANNNNTTC 1 cut(s) 788
PfeI GAWTC 3 cut(s) 107, 476, 656
PinAI ACCGGT 1 cut(s) 1159
PkrI GCNGC 6 cut(s) 34, 37, 40, 340, 370, 700
PleI GAGTC 5 cut(s) 121, 619, 652, 764, 1066
PpsI GAGTC 5 cut(s) 121, 619, 652, 764, 1066
PsiI TTATAA 1 cut(s) 150
Psp124BI GAGCTC 3 cut(s) 213, 695, 1414
Psp6I CCWGG 6 cut(s) 18, 529, 581, 874, 922, 1453
PspEI GGTNACC 1 cut(s) 1117
PspGI CCWGG 6 cut(s) 18, 529, 581, 874, 922, 1453
PspN4I GGNNCC 2 cut(s) 63, 417
PspPI GGNCC 3 cut(s) 579, 926, 1421
PstI CTGCAG 1 cut(s) 633
PstNI CAGNNNCTG 2 cut(s) 338, 368
PsuI RGATCY 2 cut(s) 539, 675
PvuII CAGCTG 1 cut(s) 1148
RruI TCGCGA 1 cut(s) 723
RsaI GTAC 3 cut(s) 1037, 1139, 1511
RsaNI GTAC 3 cut(s) 1036, 1138, 1510
SacI GAGCTC 3 cut(s) 213, 695, 1414
SalI GTCGAC 1 cut(s) 491
SapI GCTCTTC 2 cut(s) 131, 765
SaqAI TTAA 1 cut(s) 869
SatI GCNGC 6 cut(s) 33, 36, 39, 339, 369, 699
Sau3AI GATC 7 cut(s) 175, 539, 675, 685, 718, 1250, 1404
Sau96I GGNCC 3 cut(s) 579, 926, 1421
SchI GAGTC 5 cut(s) 122, 619, 653, 765, 1066
ScrFI CCNGG 6 cut(s) 20, 531, 583, 876, 924, 1455
SduI GDGCHC 8 cut(s) 143, 213, 270, 389, 564, 695, 975, 1414
SfaNI GCATC 1 cut(s) 603
SfcI CTRYAG 1 cut(s) 629
SinI GGWCC 1 cut(s) 926
SmlI CTYRAG 6 cut(s) 179, 574, 820, 911, 1133, 1241
SmoI CTYRAG 6 cut(s) 179, 574, 820, 911, 1133, 1241
SseBI AGGCCT 1 cut(s) 874
SsiI CCGC 6 cut(s) 26, 32, 35, 38, 296, 1110
SspI AATATT 1 cut(s) 1012
SspMI CTAG 1 cut(s) 1359
SstI GAGCTC 3 cut(s) 213, 695, 1414
StuI AGGCCT 1 cut(s) 874
StyD4I CCNGG 6 cut(s) 18, 529, 581, 874, 922, 1453
TaaI ACNGT 2 cut(s) 7, 130
TaqI TCGA 9 cut(s) 218, 225, 309, 474, 492, 609, 647, 1476, 1487
TatI WGTACW 1 cut(s) 1137
TauI GCSGC 3 cut(s) 35, 38, 41
TfiI GAWTC 3 cut(s) 107, 476, 656
Tru1I TTAA 1 cut(s) 869
Tru9I TTAA 1 cut(s) 869
TscAI CASTG 2 cut(s) 133, 638
TseFI GTSAC 1 cut(s) 1117
TseI GCWGC 3 cut(s) 338, 368, 698
Tsp45I GTSAC 1 cut(s) 1117
TspDTI ATGAA 5 cut(s) 474, 813, 1006, 1380, 1431
TspRI CASTG 2 cut(s) 133, 638
VpaK11BI GGWCC 1 cut(s) 926
XagI CCTNNNNNAGG 1 cut(s) 534
XapI RAATTY 1 cut(s) 569
XmiI GTMKAC 1 cut(s) 492
XmnI GAANNNNTTC 1 cut(s) 788
XspI CTAG 1 cut(s) 1359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.