Rorug05G0041000

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001644
Physical Location & Seq
Reverse (-)
3534978 .. 3535840
863 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0041000.1

Sequence Viewer

Length: 774 bp
ATGACCCAAGAGCCACGAACTCTGCAACTAATGGAGCTCCAAACCCCGCAACAAATGGAACCACAACAACAGCAACCAACAGAGGAGGGAGGAAACACCCAAGCAGCTGGAAGTAGTGCCAACATGGATTTCTGGCGAAGCCGTACCAGGTGGATTCCTACTCCAGAACAAATAAGAGTCCTCAAGGATCTTTACTACGTCAAGGGATTTAAGTGCCCAACTACAGAGCACATTCACGAGATCTGCCTCCAGCTGAACCAGTATGGACATGTTGAGGGTAAGAACATTTACTTTTGGTTCCAGAACGTCAGGGCTCGAGAGAAGCAGATGAATAGGTGTAAACAGGCTGCTCCAGTGCCCATGGAAACTAGTTCTCTTGGTACTGGTGGATCCATTGATCTCAATTTTGGGTCCACTGGTTCTACTGGTGCTGGTGGATCCATCGACATCAATTTTGGGCCAGCTGGTGGATCCATTGACATCAATTTTGGGTCCACTAGTTCTACTGATAATGGTAGATCCATTGATCTCAACTTTGGTTCCACCTATTCTACTGGAGGACGAACATCCCTGCAACAACGAGGAGGAGATCACCAGGAGGTTGAAACTCTTCCTCTGTTCCCCGTGCATGGCGAGGACATCTTTAGTAACCCGAAGACTACATCCGAGGAAGGTAGCGTATTTGGTTACTATTCTGGTGGCTCAGGCGGTTACAACAGTGGCTCTAACGTTTCTCTTGAGCTCAGCCTCAATCCATCCGGAGCTGCTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

257

Amino Acids

27.88

Weight (kDa)

5.05

Isoelectric Point (pI)

45.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 48 - 109 2.1e-09 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 467
AccIII TCCGGA 1 cut(s) 758
AciI CCGC 2 cut(s) 47, 708
AclI AACGTT 1 cut(s) 729
AclWI GGATC 8 cut(s) 195, 384, 397, 432, 445, 465, 478, 513
AfaI GTAC 2 cut(s) 145, 382
AfiI CCNNNNNNNGG 2 cut(s) 467, 629
AflIII ACRYGT 1 cut(s) 268
AgsI TTSAA 1 cut(s) 605
AhlI ACTAGT 2 cut(s) 368, 497
AjnI CCWGG 2 cut(s) 146, 594
AjuI GAANNNNNNNTTGG 2 cut(s) 113, 145
AluBI AGCT 6 cut(s) 37, 107, 253, 464, 742, 764
AluI AGCT 6 cut(s) 37, 107, 253, 464, 742, 764
Alw21I GWGCWC 3 cut(s) 39, 231, 744
AlwI GGATC 8 cut(s) 195, 384, 397, 432, 445, 465, 478, 513
Ama87I CYCGRG 1 cut(s) 315
Aor13HI TCCGGA 1 cut(s) 758
AoxI GGCC 1 cut(s) 458
ApeKI GCWGC 3 cut(s) 104, 347, 764
ArsI GACNNNNNNTTYG 4 cut(s) 437, 469, 470, 502
Asp700I GAANNNNTTC 1 cut(s) 609
AspS9I GGNCC 3 cut(s) 411, 458, 492
AsuHPI GGTGA 1 cut(s) 584
AvaI CYCGRG 1 cut(s) 315
AvaII GGWCC 2 cut(s) 411, 492
BaeGI GKGCMC 2 cut(s) 218, 360
BamHI GGATCC 3 cut(s) 389, 437, 470
BanII GRGCYC 3 cut(s) 39, 316, 744
BauI CACGAG 1 cut(s) 236
BbsI GAAGAC 1 cut(s) 662
Bbv12I GWGCWC 3 cut(s) 39, 231, 744
BbvI GCAGC 3 cut(s) 116, 334, 751
BccI CCATC 2 cut(s) 449, 763
BceAI ACGGC 1 cut(s) 126
BciT130I CCWGG 2 cut(s) 148, 596
BcuI ACTAGT 2 cut(s) 368, 497
BfaI CTAG 3 cut(s) 369, 498, 772
BfmI CTRYAG 1 cut(s) 222
BglII AGATCT 1 cut(s) 240
BisI GCNGC 3 cut(s) 105, 348, 765
BlpI GCTNAGC 1 cut(s) 743
BlsI GCNGC 3 cut(s) 106, 349, 766
Bme1390I CCNGG 2 cut(s) 148, 596
Bme18I GGWCC 2 cut(s) 411, 492
BmeT110I CYCGRG 1 cut(s) 315
BmgT120I GGNCC 3 cut(s) 411, 458, 492
BmiI GGNNCC 8 cut(s) 60, 299, 391, 412, 439, 472, 493, 541
BmrFI CCNGG 2 cut(s) 148, 596
BpiI GAAGAC 1 cut(s) 662
BpmI CTGGAG 4 cut(s) 147, 233, 336, 576
Bpu10I CCTNAGC 1 cut(s) 703
Bpu1102I GCTNAGC 1 cut(s) 743
BpuEI CTTGAG 2 cut(s) 167, 758
BsaJI CCNNGG 2 cut(s) 360, 666
BsaWI WCCGGW 1 cut(s) 758
Bsc4I CCNNNNNNNGG 2 cut(s) 467, 629
Bse1I ACTGG 6 cut(s) 259, 353, 388, 421, 430, 559
BseAI TCCGGA 1 cut(s) 758
BseBI CCWGG 2 cut(s) 148, 596
BseDI CCNNGG 2 cut(s) 360, 666
BseGI GGATG 3 cut(s) 566, 662, 755
BseLI CCNNNNNNNGG 2 cut(s) 467, 629
BseMII CTCAG 2 cut(s) 717, 757
BseNI ACTGG 6 cut(s) 259, 353, 388, 421, 430, 559
BseRI GAGGAG 3 cut(s) 98, 597, 600
BseSI GKGCMC 2 cut(s) 218, 360
BseXI GCAGC 3 cut(s) 116, 334, 751
BshFI GGCC 1 cut(s) 460
BsiHKAI GWGCWC 3 cut(s) 39, 231, 744
BsiHKCI CYCGRG 1 cut(s) 315
BsiSI CCGG 1 cut(s) 759
BslI CCNNNNNNNGG 2 cut(s) 467, 629
BsnI GGCC 1 cut(s) 460
BsoBI CYCGRG 1 cut(s) 315
Bsp1286I GDGCHC 6 cut(s) 39, 218, 231, 316, 360, 744
Bsp13I TCCGGA 1 cut(s) 758
Bsp143I GATC 9 cut(s) 187, 240, 389, 397, 437, 470, 518, 526, 589
Bsp1720I GCTNAGC 1 cut(s) 743
Bsp19I CCATGG 1 cut(s) 360
BspACI CCGC 2 cut(s) 47, 708
BspANI GGCC 1 cut(s) 460
BspCNI CTCAG 2 cut(s) 716, 756
BspEI TCCGGA 1 cut(s) 758
BspLI GGNNCC 8 cut(s) 60, 299, 391, 412, 439, 472, 493, 541
BspPI GGATC 8 cut(s) 195, 384, 397, 432, 445, 465, 478, 513
BsrI ACTGG 6 cut(s) 259, 353, 388, 421, 430, 559
BssECI CCNNGG 2 cut(s) 360, 666
BssMI GATC 9 cut(s) 187, 240, 389, 397, 437, 470, 518, 526, 589
BssSI CACGAG 1 cut(s) 236
BssT1I CCWWGG 1 cut(s) 360
Bst2BI CACGAG 1 cut(s) 236
Bst2UI CCWGG 2 cut(s) 148, 596
Bst4CI ACNGT 1 cut(s) 719
Bst6I CTCTTC 1 cut(s) 615
BstC8I GCNNGC 1 cut(s) 462
BstDEI CTNAG 2 cut(s) 703, 743
BstDSI CCRYGG 1 cut(s) 360
BstF5I GGATG 3 cut(s) 566, 662, 755
BstKTI GATC 9 cut(s) 190, 243, 392, 400, 440, 473, 521, 529, 592
BstMBI GATC 9 cut(s) 187, 240, 389, 397, 437, 470, 518, 526, 589
BstNI CCWGG 2 cut(s) 148, 596
BstNSI RCATGY 1 cut(s) 272
BstSCI CCNGG 2 cut(s) 146, 594
BstSFI CTRYAG 1 cut(s) 222
BstSLI GKGCMC 2 cut(s) 218, 360
BstV1I GCAGC 3 cut(s) 116, 334, 751
BstV2I GAAGAC 1 cut(s) 662
BstX2I RGATCY 6 cut(s) 187, 240, 389, 437, 470, 518
BstXI CCANNNNNNTGG 1 cut(s) 107
BstYI RGATCY 6 cut(s) 187, 240, 389, 437, 470, 518
BsuRI GGCC 1 cut(s) 460
BtgI CCRYGG 1 cut(s) 360
BtsCI GGATG 3 cut(s) 566, 662, 755
BtsIMutI CAGTG 3 cut(s) 360, 414, 724
Cac8I GCNNGC 1 cut(s) 462
Cfr13I GGNCC 3 cut(s) 411, 458, 492
CsiI ACCWGGT 1 cut(s) 146
Csp6I GTAC 2 cut(s) 144, 381
CviAII CATG 4 cut(s) 124, 269, 361, 629
CviQI GTAC 2 cut(s) 144, 381
DdeI CTNAG 2 cut(s) 703, 743
DpnI GATC 9 cut(s) 189, 242, 391, 399, 439, 472, 520, 528, 591
DpnII GATC 9 cut(s) 187, 240, 389, 397, 437, 470, 518, 526, 589
Eam1104I CTCTTC 1 cut(s) 615
EarI CTCTTC 1 cut(s) 615
Ecl136II GAGCTC 2 cut(s) 37, 742
Eco130I CCWWGG 1 cut(s) 360
Eco24I GRGCYC 3 cut(s) 39, 316, 744
Eco47I GGWCC 2 cut(s) 411, 492
Eco53kI GAGCTC 2 cut(s) 37, 742
Eco88I CYCGRG 1 cut(s) 315
EcoICRI GAGCTC 2 cut(s) 37, 742
EcoRII CCWGG 2 cut(s) 146, 594
EcoT14I CCWWGG 1 cut(s) 360
EcoT38I GRGCYC 3 cut(s) 39, 316, 744
ErhI CCWWGG 1 cut(s) 360
FaeI CATG 4 cut(s) 127, 272, 364, 632
FaiI YATR 5 cut(s) 125, 264, 270, 362, 630
FatI CATG 4 cut(s) 123, 268, 360, 628
FauI CCCGC 1 cut(s) 54
Fnu4HI GCNGC 3 cut(s) 105, 348, 765
FokI GGATG 3 cut(s) 553, 649, 742
FriOI GRGCYC 3 cut(s) 39, 316, 744
Fsp4HI GCNGC 3 cut(s) 105, 348, 765
FspBI CTAG 3 cut(s) 369, 498, 772
GluI GCNGC 3 cut(s) 105, 348, 765
GsuI CTGGAG 4 cut(s) 147, 233, 336, 576
HaeIII GGCC 1 cut(s) 460
HapII CCGG 1 cut(s) 759
Hin1II CATG 4 cut(s) 127, 272, 364, 632
HinfI GANTC 2 cut(s) 154, 177
HpaII CCGG 1 cut(s) 759
HphI GGTGA 1 cut(s) 584
Hpy166II GTNNAC 3 cut(s) 341, 414, 495
Hpy188I TCNGA 1 cut(s) 667
Hpy188III TCNNGA 6 cut(s) 164, 236, 301, 317, 737, 759
Hpy8I GTNNAC 3 cut(s) 341, 414, 495
HpyAV CCTTC 1 cut(s) 665
HpyCH4III ACNGT 1 cut(s) 719
HpyCH4IV ACGT 3 cut(s) 198, 306, 729
HpyCH4V TGCA 3 cut(s) 25, 574, 628
HpyF3I CTNAG 2 cut(s) 703, 743
HpySE526I ACGT 3 cut(s) 198, 306, 729
Hsp92II CATG 4 cut(s) 127, 272, 364, 632
Kpn2I TCCGGA 1 cut(s) 758
Kzo9I GATC 9 cut(s) 187, 240, 389, 397, 437, 470, 518, 526, 589
LmnI GCTCC 4 cut(s) 34, 42, 355, 761
Lsp1109I GCAGC 3 cut(s) 116, 334, 751
MabI ACCWGGT 1 cut(s) 146
MaeI CTAG 3 cut(s) 369, 498, 772
MaeII ACGT 3 cut(s) 198, 306, 729
MaeIII GTNAC 3 cut(s) 647, 686, 710
MalI GATC 9 cut(s) 189, 242, 391, 399, 439, 472, 520, 528, 591
MboI GATC 9 cut(s) 187, 240, 389, 397, 437, 470, 518, 526, 589
MboII GAAGA 2 cut(s) 602, 667
MflI RGATCY 6 cut(s) 187, 240, 389, 437, 470, 518
MhlI GDGCHC 6 cut(s) 39, 218, 231, 316, 360, 744
MluCI AATT 3 cut(s) 403, 451, 484
MlyI GAGTC 1 cut(s) 186
MroI TCCGGA 1 cut(s) 758
MroXI GAANNNNTTC 1 cut(s) 609
MseI TTAA 1 cut(s) 210
MspA1I CMGCKG 3 cut(s) 107, 253, 464
MspI CCGG 1 cut(s) 759
MspR9I CCNGG 2 cut(s) 148, 596
MvaI CCWGG 2 cut(s) 148, 596
NcoI CCATGG 1 cut(s) 360
NdeII GATC 9 cut(s) 187, 240, 389, 397, 437, 470, 518, 526, 589
NlaIII CATG 4 cut(s) 127, 272, 364, 632
NlaIV GGNNCC 8 cut(s) 60, 299, 391, 412, 439, 472, 493, 541
NspI RCATGY 1 cut(s) 272
PaeR7I CTCGAG 1 cut(s) 315
PciI ACATGT 1 cut(s) 268
PdmI GAANNNNTTC 1 cut(s) 609
PfeI GAWTC 1 cut(s) 154
PflMI CCANNNNNTGG 1 cut(s) 467
PkrI GCNGC 3 cut(s) 106, 349, 766
PleI GAGTC 1 cut(s) 185
PpsI GAGTC 1 cut(s) 185
PscI ACATGT 1 cut(s) 268
Psp124BI GAGCTC 2 cut(s) 39, 744
Psp1406I AACGTT 1 cut(s) 729
Psp6I CCWGG 2 cut(s) 146, 594
PspGI CCWGG 2 cut(s) 146, 594
PspN4I GGNNCC 8 cut(s) 60, 299, 391, 412, 439, 472, 493, 541
PspPI GGNCC 3 cut(s) 411, 458, 492
PsuI RGATCY 6 cut(s) 187, 240, 389, 437, 470, 518
PvuII CAGCTG 3 cut(s) 107, 253, 464
RsaI GTAC 2 cut(s) 145, 382
RsaNI GTAC 2 cut(s) 144, 381
SacI GAGCTC 2 cut(s) 39, 744
SaqAI TTAA 1 cut(s) 210
SatI GCNGC 3 cut(s) 105, 348, 765
Sau3AI GATC 9 cut(s) 187, 240, 389, 397, 437, 470, 518, 526, 589
Sau96I GGNCC 3 cut(s) 411, 458, 492
SchI GAGTC 1 cut(s) 186
ScrFI CCNGG 2 cut(s) 148, 596
SduI GDGCHC 6 cut(s) 39, 218, 231, 316, 360, 744
SexAI ACCWGGT 1 cut(s) 146
SfcI CTRYAG 1 cut(s) 222
Sfr274I CTCGAG 1 cut(s) 315
SinI GGWCC 2 cut(s) 411, 492
SlaI CTCGAG 1 cut(s) 315
SmlI CTYRAG 3 cut(s) 182, 315, 737
SmoI CTYRAG 3 cut(s) 182, 315, 737
SpeI ACTAGT 2 cut(s) 368, 497
Sse9I AATT 3 cut(s) 403, 451, 484
SsiI CCGC 2 cut(s) 47, 708
SspMI CTAG 3 cut(s) 369, 498, 772
SstI GAGCTC 2 cut(s) 39, 744
StyD4I CCNGG 2 cut(s) 146, 594
StyI CCWWGG 1 cut(s) 360
TaaI ACNGT 1 cut(s) 719
TaiI ACGT 3 cut(s) 201, 309, 732
TaqI TCGA 2 cut(s) 316, 444
TasI AATT 3 cut(s) 403, 451, 484
TfiI GAWTC 1 cut(s) 154
Tru1I TTAA 1 cut(s) 210
Tru9I TTAA 1 cut(s) 210
TscAI CASTG 3 cut(s) 360, 421, 724
TseI GCWGC 3 cut(s) 104, 347, 764
TspDTI ATGAA 1 cut(s) 344
TspRI CASTG 3 cut(s) 360, 421, 724
Van91I CCANNNNNTGG 1 cut(s) 467
VpaK11BI GGWCC 2 cut(s) 411, 492
XceI RCATGY 1 cut(s) 272
XhoI CTCGAG 1 cut(s) 315
XmnI GAANNNNTTC 1 cut(s) 609
XspI CTAG 3 cut(s) 369, 498, 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.