Rh6AG303200

Patellin-4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0001644
Physical Location & Seq
Forward (+)
50188675 .. 50193407
4733 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG303200.1

Sequence Viewer

Length: 1392 bp
ATGACGGAGAAGAATGATCAGGAAACCCACAATGACAATGAGCCTGATTATCATGGCAGAGACCAAGAGCTCGACTCGGCTCTGGGATATTATATTAATGACATGTTGATGGAAAGCCCCCGCGAATTATGTCCAGATAGTGACCAAGAAGGTGATGAACCTACAGCTCAAGAGGATGAGGAAACCAAAGCTGCTTTTGTGGAAATGAAGAAGAAGAAGAAGAAGAAAGCCTTGATGGAATTCCGGTGCAGAGTTGAAGATGCAATTGTGGGGAATTACCTTCTTGGAAAACCCAGCAGAAAGCTTTGCCGAGATGAAAGCTTGAGGCAAAAAGAACGACTCAAGGAGATTACCCTATGGGGTGTGCCTTTATTGCCAAGTAAAGGCCACAAAGGCACTGATGCTGTGCTCCTCAAATTCTTGAGAGCCCGGGATTTCAAGACTACCGACGCATTTGAAATGCTGAGAAGAACCCTGAAATGGCGAAAAGAATACAGGGCTGATGAAATTCTTGAAGAAGAATTGGGAGGCCCTGATCTTGATAATGTGGTGTGCATAAAGAGTAGAGACAAAGAGGGTCACCCCTTGTGTTACACTGTGTATGGACCTTTCAAGGACAAAGAGTTGTACGAGAAAACGTTCGGGTCTGAAGCGAAACGCCAACAGTTTTTGAGGTGGAGAATTCAATTCATCGAAAAGGGAATCAAGAAGCTCAACTTCAAGAGAGGGGTTGATTCGATGGTTCAGATTACGGATTTGAAGAACTCCCCAGGCCCCGATTTTAAGGAGCTTCGTTCGCTTAGTAGCAAAACTCTGCTCTTGTTACAGGAAAACTATCCTGAGCTCATTCAAAAGAATATTGTTATAAATGCTCCACTTTGGTACTATGTGCTCCATGTGCTCCGATCAAGGTTTCTAAGTCACAGAACTAAGAAGAAGTTCGTCTTTGCTAGACCCCAAAAGGTTACAAAGACCCTTCTCAAGTTCATAGAACCAGAAGAACTCCCAGTTCAATATGGTGGCCTCAAAAGAGAGCAGGATGAGGATTTTACACCAGAGGACAAAGTATCACAGATCATAGTCAAAGGAAACACAACTAATTATATTAAAATTCCAGTTGCTGAGGGTGGATTGACAATGGTGTGGGATCTAACCGTGGTGGGATGGGACGTGTTGTACAAGGAAGAGTTCGTTCCTGACGATGAAGGGTCATACAAGATTTTACTCCAAGATAAGAAGAGACTGCAAGGGGAGAGTGTAAGAAACTCCTTCTACCTTAGTGAACCAGGGAAGATAATCATTGCCCTTGAGAATTGGAATTTCAAGAACAGGAGGGTTCTTTATCGGTATAAAGCCAAACCCACCATTCCCATTTACTTAACTAATAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

463

Amino Acids

54.28

Weight (kDa)

8.79

Isoelectric Point (pI)

43.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_N PF03765 116 - 156 1.1e-07 CRAL/TRIO, N-terminal domain
CRAL_TRIO PF00650 183 - 341 7.1e-26 CRAL/TRIO domain
GOLD_PATL1_C PF25099 362 - 443 7.6e-29 Patellin-1-like, C-terminal GOLD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 866
AccII CGCG 1 cut(s) 123
AciI CCGC 1 cut(s) 121
AclI AACGTT 1 cut(s) 638
AclWI GGATC 1 cut(s) 1155
AcsI RAATTY 6 cut(s) 239, 416, 507, 681, 1110, 1318
AcuI CTGAAG 1 cut(s) 669
AfaI GTAC 3 cut(s) 629, 884, 1178
AfiI CCNNNNNNNGG 2 cut(s) 383, 480
AflIII ACRYGT 2 cut(s) 102, 1170
AjiI CACGTC 1 cut(s) 1171
AjnI CCWGG 2 cut(s) 769, 1285
AluBI AGCT 8 cut(s) 70, 167, 191, 304, 321, 712, 790, 844
AluI AGCT 8 cut(s) 70, 167, 191, 304, 321, 712, 790, 844
Alw21I GWGCWC 5 cut(s) 72, 411, 846, 894, 903
Alw26I GTCTC 3 cut(s) 54, 561, 1234
AlwI GGATC 1 cut(s) 1155
AlwNI CAGNNNCTG 1 cut(s) 1121
Ama87I CYCGRG 1 cut(s) 429
AoxI GGCC 4 cut(s) 385, 529, 772, 1021
ApeKI GCWGC 1 cut(s) 191
ApoI RAATTY 6 cut(s) 239, 416, 507, 681, 1110, 1318
AseI ATTAAT 1 cut(s) 96
Asp700I GAANNNNTTC 2 cut(s) 638, 938
AspS9I GGNCC 3 cut(s) 530, 605, 773
AsuC2I CCSGG 2 cut(s) 430, 431
AsuHPI GGTGA 2 cut(s) 164, 572
AvaI CYCGRG 1 cut(s) 429
AvaII GGWCC 1 cut(s) 605
BanII GRGCYC 3 cut(s) 72, 430, 846
Bbv12I GWGCWC 5 cut(s) 72, 411, 846, 894, 903
BbvCI CCTCAGC 1 cut(s) 1122
BbvI GCAGC 1 cut(s) 178
BccI CCATC 4 cut(s) 103, 229, 733, 1158
BciT130I CCWGG 2 cut(s) 771, 1287
BclI TGATCA 1 cut(s) 16
BcnI CCSGG 2 cut(s) 430, 431
BcoDI GTCTC 3 cut(s) 54, 561, 1234
BfaI CTAG 1 cut(s) 951
BfmI CTRYAG 1 cut(s) 162
BglI GCCNNNNNGGC 1 cut(s) 393
BisI GCNGC 1 cut(s) 192
BlsI GCNGC 1 cut(s) 193
Bme1390I CCNGG 4 cut(s) 430, 431, 771, 1287
Bme18I GGWCC 1 cut(s) 605
BmeT110I CYCGRG 1 cut(s) 429
BmgBI CACGTC 1 cut(s) 1171
BmgT120I GGNCC 3 cut(s) 530, 605, 773
BmiI GGNNCC 1 cut(s) 775
BmrFI CCNGG 4 cut(s) 430, 431, 771, 1287
BmrI ACTGGG 1 cut(s) 1001
BmsI GCATC 2 cut(s) 250, 391
BmuI ACTGGG 1 cut(s) 1001
BplI GAGNNNNNCTC 2 cut(s) 59, 91
Bpu10I CCTNAGC 2 cut(s) 840, 1122
BpuEI CTTGAG 6 cut(s) 153, 326, 343, 442, 965, 1328
BpuMI CCSGG 2 cut(s) 430, 431
BsaI GGTCTC 1 cut(s) 54
BsaJI CCNNGG 4 cut(s) 429, 769, 1155, 1286
BsaWI WCCGGW 1 cut(s) 243
Bsc4I CCNNNNNNNGG 2 cut(s) 383, 480
Bse1I ACTGG 2 cut(s) 1007, 1115
Bse3DI GCAATG 1 cut(s) 1299
BseBI CCWGG 2 cut(s) 771, 1287
BseDI CCNNGG 4 cut(s) 429, 769, 1155, 1286
BseGI GGATG 3 cut(s) 181, 1045, 1169
BseLI CCNNNNNNNGG 2 cut(s) 383, 480
BseMI GCAATG 1 cut(s) 1299
BseMII CTCAG 3 cut(s) 455, 831, 1113
BseNI ACTGG 2 cut(s) 1007, 1115
BseRI GAGGAG 1 cut(s) 401
BseXI GCAGC 1 cut(s) 178
BseYI CCCAGC 1 cut(s) 293
BsgI GTGCAG 1 cut(s) 268
Bsh1236I CGCG 1 cut(s) 123
BshFI GGCC 4 cut(s) 387, 531, 774, 1023
BsiHKAI GWGCWC 5 cut(s) 72, 411, 846, 894, 903
BsiHKCI CYCGRG 1 cut(s) 429
BsiSI CCGG 2 cut(s) 244, 430
BslFI GGGAC 1 cut(s) 1181
BslI CCNNNNNNNGG 2 cut(s) 383, 480
BsmAI GTCTC 3 cut(s) 54, 561, 1234
BsmFI GGGAC 1 cut(s) 1181
BsnI GGCC 4 cut(s) 387, 531, 774, 1023
Bso31I GGTCTC 1 cut(s) 54
BsoBI CYCGRG 1 cut(s) 429
Bsp1286I GDGCHC 6 cut(s) 72, 411, 430, 846, 894, 903
Bsp1407I TGTACA 1 cut(s) 1176
Bsp143I GATC 5 cut(s) 16, 535, 905, 1074, 1147
BspACI CCGC 1 cut(s) 121
BspANI GGCC 4 cut(s) 387, 531, 774, 1023
BspCNI CTCAG 3 cut(s) 456, 832, 1114
BspFNI CGCG 1 cut(s) 123
BspLI GGNNCC 1 cut(s) 775
BspPI GGATC 1 cut(s) 1155
BspTNI GGTCTC 1 cut(s) 54
BsrDI GCAATG 1 cut(s) 1299
BsrGI TGTACA 1 cut(s) 1176
BsrI ACTGG 2 cut(s) 1007, 1115
BssECI CCNNGG 4 cut(s) 429, 769, 1155, 1286
BssMI GATC 5 cut(s) 16, 535, 905, 1074, 1147
Bst2UI CCWGG 2 cut(s) 771, 1287
Bst4CI ACNGT 3 cut(s) 598, 666, 1156
Bst6I CTCTTC 2 cut(s) 1179, 1232
BstAUI TGTACA 1 cut(s) 1176
BstDEI CTNAG 7 cut(s) 464, 800, 840, 917, 930, 1122, 1277
BstDSI CCRYGG 1 cut(s) 1155
BstEII GGTNACC 1 cut(s) 578
BstF5I GGATG 3 cut(s) 181, 1045, 1169
BstFNI CGCG 1 cut(s) 123
BstKTI GATC 5 cut(s) 19, 538, 908, 1077, 1150
BstMAI GTCTC 3 cut(s) 54, 561, 1234
BstMBI GATC 5 cut(s) 16, 535, 905, 1074, 1147
BstMWI GCNNNNNNNGC 4 cut(s) 373, 393, 796, 898
BstNI CCWGG 2 cut(s) 771, 1287
BstNSI RCATGY 1 cut(s) 106
BstPI GGTNACC 1 cut(s) 578
BstSCI CCNGG 4 cut(s) 428, 429, 769, 1285
BstSFI CTRYAG 1 cut(s) 162
BstUI CGCG 1 cut(s) 123
BstV1I GCAGC 1 cut(s) 178
BstX2I RGATCY 1 cut(s) 1147
BstYI RGATCY 1 cut(s) 1147
BsuRI GGCC 4 cut(s) 387, 531, 774, 1023
BtgI CCRYGG 1 cut(s) 1155
BtrI CACGTC 1 cut(s) 1171
BtsCI GGATG 3 cut(s) 181, 1045, 1169
BtsIMutI CAGTG 2 cut(s) 396, 594
CaiI CAGNNNCTG 1 cut(s) 1121
Cfr13I GGNCC 3 cut(s) 530, 605, 773
Cfr9I CCCGGG 1 cut(s) 429
CseI GACGC 1 cut(s) 458
Csp6I GTAC 3 cut(s) 628, 883, 1177
CviAII CATG 3 cut(s) 53, 103, 896
CviQI GTAC 3 cut(s) 628, 883, 1177
DdeI CTNAG 7 cut(s) 464, 800, 840, 917, 930, 1122, 1277
DpnI GATC 5 cut(s) 18, 537, 907, 1076, 1149
DpnII GATC 5 cut(s) 16, 535, 905, 1074, 1147
Eam1104I CTCTTC 2 cut(s) 1179, 1232
EarI CTCTTC 2 cut(s) 1179, 1232
Ecl136II GAGCTC 2 cut(s) 70, 844
Eco24I GRGCYC 3 cut(s) 72, 430, 846
Eco31I GGTCTC 1 cut(s) 54
Eco47I GGWCC 1 cut(s) 605
Eco53kI GAGCTC 2 cut(s) 70, 844
Eco57I CTGAAG 1 cut(s) 669
Eco88I CYCGRG 1 cut(s) 429
Eco91I GGTNACC 1 cut(s) 578
EcoICRI GAGCTC 2 cut(s) 70, 844
EcoO109I RGGNCCY 2 cut(s) 530, 773
EcoO65I GGTNACC 1 cut(s) 578
EcoRI GAATTC 2 cut(s) 239, 681
EcoRII CCWGG 2 cut(s) 769, 1285
EcoT38I GRGCYC 3 cut(s) 72, 430, 846
FaeI CATG 3 cut(s) 56, 106, 899
FalI AAGNNNNNCTT 6 cut(s) 215, 247, 701, 733, 929, 961
FaqI GGGAC 1 cut(s) 1181
FatI CATG 3 cut(s) 52, 102, 895
FauI CCCGC 1 cut(s) 128
FbaI TGATCA 1 cut(s) 16
Fnu4HI GCNGC 1 cut(s) 192
FokI GGATG 3 cut(s) 188, 1052, 1176
FriOI GRGCYC 3 cut(s) 72, 430, 846
Fsp4HI GCNGC 1 cut(s) 192
FspBI CTAG 1 cut(s) 951
GluI GCNGC 1 cut(s) 192
GsaI CCCAGC 1 cut(s) 297
HaeIII GGCC 4 cut(s) 387, 531, 774, 1023
HapII CCGG 2 cut(s) 244, 430
HgaI GACGC 1 cut(s) 458
Hin1II CATG 3 cut(s) 56, 106, 899
HindIII AAGCTT 2 cut(s) 302, 319
HinfI GANTC 4 cut(s) 74, 339, 702, 734
HpaII CCGG 2 cut(s) 244, 430
HphI GGTGA 2 cut(s) 164, 572
Hpy166II GTNNAC 1 cut(s) 1283
Hpy188I TCNGA 3 cut(s) 649, 747, 905
Hpy8I GTNNAC 1 cut(s) 1283
Hpy99I CGWCG 1 cut(s) 452
HpyAV CCTTC 5 cut(s) 143, 290, 986, 1199, 1279
HpyCH4III ACNGT 3 cut(s) 598, 666, 1156
HpyCH4IV ACGT 2 cut(s) 638, 1170
HpyCH4V TGCA 4 cut(s) 249, 263, 555, 1246
HpyF10VI GCNNNNNNNGC 4 cut(s) 373, 393, 796, 898
HpyF3I CTNAG 7 cut(s) 464, 800, 840, 917, 930, 1122, 1277
HpySE526I ACGT 2 cut(s) 638, 1170
Hsp92II CATG 3 cut(s) 56, 106, 899
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 5 cut(s) 16, 535, 905, 1074, 1147
LmnI GCTCC 5 cut(s) 414, 787, 877, 897, 906
Lsp1109I GCAGC 1 cut(s) 178
LweI GCATC 2 cut(s) 250, 391
MaeI CTAG 1 cut(s) 951
MaeII ACGT 2 cut(s) 638, 1170
MaeIII GTNAC 6 cut(s) 140, 578, 590, 822, 920, 964
MalI GATC 5 cut(s) 18, 537, 907, 1076, 1149
MboI GATC 5 cut(s) 16, 535, 905, 1074, 1147
MfeI CAATTG 1 cut(s) 264
MflI RGATCY 1 cut(s) 1147
MhlI GDGCHC 6 cut(s) 72, 411, 430, 846, 894, 903
MlyI GAGTC 2 cut(s) 68, 333
MroXI GAANNNNTTC 2 cut(s) 638, 938
MseI TTAA 4 cut(s) 96, 783, 1107, 1379
MslI CAYNNNNRTG 1 cut(s) 107
MspI CCGG 2 cut(s) 244, 430
MspR9I CCNGG 4 cut(s) 430, 431, 771, 1287
MunI CAATTG 1 cut(s) 264
MvaI CCWGG 2 cut(s) 771, 1287
MvnI CGCG 1 cut(s) 123
MwoI GCNNNNNNNGC 4 cut(s) 373, 393, 796, 898
NciI CCSGG 2 cut(s) 430, 431
NdeII GATC 5 cut(s) 16, 535, 905, 1074, 1147
NlaIII CATG 3 cut(s) 56, 106, 899
NlaIV GGNNCC 1 cut(s) 775
NmeAIII GCCGAG 2 cut(s) 56, 335
NmuCI GTSAC 3 cut(s) 140, 578, 920
NspI RCATGY 1 cut(s) 106
PciI ACATGT 1 cut(s) 102
PcsI WCGNNNNNNNCGW 1 cut(s) 1197
PdmI GAANNNNTTC 2 cut(s) 638, 938
PfeI GAWTC 2 cut(s) 702, 734
PkrI GCNGC 1 cut(s) 193
PleI GAGTC 2 cut(s) 68, 333
PpsI GAGTC 2 cut(s) 68, 333
PscI ACATGT 1 cut(s) 102
PshBI ATTAAT 1 cut(s) 96
PsiI TTATAA 1 cut(s) 866
Psp124BI GAGCTC 2 cut(s) 72, 846
Psp1406I AACGTT 1 cut(s) 638
Psp6I CCWGG 2 cut(s) 769, 1285
PspEI GGTNACC 1 cut(s) 578
PspFI CCCAGC 1 cut(s) 293
PspGI CCWGG 2 cut(s) 769, 1285
PspN4I GGNNCC 1 cut(s) 775
PspPI GGNCC 3 cut(s) 530, 605, 773
PstNI CAGNNNCTG 1 cut(s) 1121
PsuI RGATCY 1 cut(s) 1147
RsaI GTAC 3 cut(s) 629, 884, 1178
RsaNI GTAC 3 cut(s) 628, 883, 1177
RseI CAYNNNNRTG 1 cut(s) 107
SacI GAGCTC 2 cut(s) 72, 846
SaqAI TTAA 4 cut(s) 96, 783, 1107, 1379
SatI GCNGC 1 cut(s) 192
Sau3AI GATC 5 cut(s) 16, 535, 905, 1074, 1147
Sau96I GGNCC 3 cut(s) 530, 605, 773
SchI GAGTC 2 cut(s) 68, 333
ScrFI CCNGG 4 cut(s) 430, 431, 771, 1287
SduI GDGCHC 6 cut(s) 72, 411, 430, 846, 894, 903
SfaNI GCATC 2 cut(s) 250, 391
SfcI CTRYAG 1 cut(s) 162
SinI GGWCC 1 cut(s) 605
SmaI CCCGGG 1 cut(s) 431
SmiMI CAYNNNNRTG 1 cut(s) 107
SmlI CTYRAG 6 cut(s) 168, 322, 341, 421, 980, 1307
SmoI CTYRAG 6 cut(s) 168, 322, 341, 421, 980, 1307
SsiI CCGC 1 cut(s) 121
SspI AATATT 1 cut(s) 859
SspMI CTAG 1 cut(s) 951
SstI GAGCTC 2 cut(s) 72, 846
StyD4I CCNGG 4 cut(s) 428, 429, 769, 1285
TaaI ACNGT 3 cut(s) 598, 666, 1156
TaiI ACGT 2 cut(s) 641, 1173
TaqI TCGA 3 cut(s) 72, 693, 737
TatI WGTACW 1 cut(s) 1176
TfiI GAWTC 2 cut(s) 702, 734
Tru1I TTAA 4 cut(s) 96, 783, 1107, 1379
Tru9I TTAA 4 cut(s) 96, 783, 1107, 1379
TscAI CASTG 2 cut(s) 403, 601
TseFI GTSAC 3 cut(s) 140, 578, 920
TseI GCWGC 1 cut(s) 191
Tsp45I GTSAC 3 cut(s) 140, 578, 920
TspDTI ATGAA 7 cut(s) 171, 221, 330, 519, 679, 976, 1218
TspGWI ACGGA 2 cut(s) 20, 767
TspMI CCCGGG 1 cut(s) 429
TspRI CASTG 2 cut(s) 403, 601
VpaK11BI GGWCC 1 cut(s) 605
VspI ATTAAT 1 cut(s) 96
XapI RAATTY 6 cut(s) 239, 416, 507, 681, 1110, 1318
XceI RCATGY 1 cut(s) 106
XmaI CCCGGG 1 cut(s) 429
XmnI GAANNNNTTC 2 cut(s) 638, 938
XspI CTAG 1 cut(s) 951
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.