Rh5CG144600

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0001644
Physical Location & Seq
Forward (+)
12872339 .. 12874942
2604 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG144600.1

Sequence Viewer

Length: 1476 bp
ATGACTGTGGAGGAGACCCAGGTGGCGGTGGCGGTGGCGGCTGAGGTCGTGGAGGTTGAGGAGCCGGTGAAGGAGTGCGAGAAGGCTGTTGTTGAGGAATCCGAGTCCAAGCCAACCACTGTTGAGAAGAGCACTTCTTATAAGGAGGAGAGCAACTATCTCTCTGATCTCAAGGAGTTTGAGAAGAAGGCTTTGGTTGAGCTCAAATCGAAGCTCGAAGAAGCCATTTTGGGCAACAACATTTTCAAGAAAGATGAGCCCAAGAAGGTTGAGGAGAAGGTGAAGGCCGTAGCTGACGTCGAGGAGAAGAAGGAAGAGAAAACAGAGGCTGCTGTGAAAACAACAGAGGAAACAGAGGCTGCTGTGAAAGCAGAGGAAACAGAGGCTGCTGTGAAAAAAACAGAGGAAACAGAGCCTGCTGTGAAAAAAGCAGAGGAAACAGAGGCTGTTGTGAAAGCAGAGGAAACAGAGGCTGTTGTGAAAGCAGAGGAAACAGAGGCTGTTGTGAAAGCAGAGGAAACAGAGGCTGTTGTGAAAACAGAGTGTGCTGAAAAAACAGAGGAAGGAGAAAAGGCACCAGAATTGGCTGAGAAGAAAGAAGAATTAGCAGTTCCGGTATGCGAGGAAGAAGCGAAACCCGAATCCAGCGAAGTCGTCGACACAGATATCTCACTCTGGGGTGTGCCTCTGTTCCCTGCAAAAGGATCTGAGGGAACTGATGTGGTGCTCTTGAAATTCTTGAGGGCCAGGGAGTTCAAGGTGAACGAAGCCTTCGAGATGCTGAAGAAGACTCTGCAGTGGAGGAAGGAGTCGAAAATTGATTCAATTTTGGTTGAGGAGATCTGCTCTGATCTGAGCTCTGCTGCTTACTTGAACGGTGTGGATCGCGAAGGCCACCCTGTATGCTACAACATCTTCGGAGTGTTTGACAACGAAGAGCTTTACCAGAAGACGTTCGGAAATGAAGAGAAGAGAGGCCAATTCTTGAGGTGGAGGGTTCAATTGATGGAGAAGAGTATCCAGAAGCTTGAATTAAGGCCTGGTGGTGCTACTTCTCTGCTTCAGATCAATGACCTCAAGAACTCGCCTGGGCCAATCAAGAAGGAGCTTAGGGTTGCAACAAAGCAAGCTGTTGGGCTCTTGCAGGACAATTACCCTGAATTGGTTGCCAAAAATATTTTCATCAATGTTCCATTCTGGTACTATGCACTCAATGCTCTGTTGTCTCCATTCTTGACTCAAAGAACCAAGAGCAAGTTTGTGGTGGCTCGCCCCGCTAAGGTCACCGAGACCCTTCTCAAGTACATTCCAGCTGAGGAGATACCGGTTCAGTATGGTGGCTTCAAGAGGGAAAATGACACCGAGTTCTTCTCCTCCGAAGATGGTTCTGTTTCTGAGCTAACTCTCAAGGCCGGATCAACCCAAACCATAGAGATTGATGTAGCTGAGGTACTAGTAATTTTTTTATTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

491

Amino Acids

54.94

Weight (kDa)

4.72

Isoelectric Point (pI)

54.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_N PF03765 213 - 263 4e-09 CRAL/TRIO, N-terminal domain
CRAL_TRIO PF00650 288 - 447 5.9e-24 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 141
AatII GACGTC 1 cut(s) 300
AccB1I GGYRCC 1 cut(s) 574
AccI GTMKAC 1 cut(s) 657
AccII CGCG 1 cut(s) 888
AciI CCGC 4 cut(s) 26, 32, 38, 1275
AclWI GGATC 3 cut(s) 712, 891, 1423
AcsI RAATTY 1 cut(s) 734
AcuI CTGAAG 2 cut(s) 803, 1046
AcyI GRCGYC 1 cut(s) 297
AfaI GTAC 3 cut(s) 1202, 1304, 1452
AfiI CCNNNNNNNGG 4 cut(s) 25, 701, 1162, 1279
AgeI ACCGGT 1 cut(s) 1324
AgsI TTSAA 8 cut(s) 247, 733, 757, 825, 874, 1001, 1031, 1345
AhlI ACTAGT 1 cut(s) 1453
AjnI CCWGG 4 cut(s) 18, 746, 1039, 1087
AjuI GAANNNNNNNTTGG 4 cut(s) 176, 208, 1163, 1195
Alw21I GWGCWC 4 cut(s) 134, 204, 729, 860
Alw26I GTCTC 3 cut(s) 8, 1230, 1283
AlwI GGATC 3 cut(s) 712, 891, 1423
AlwNI CAGNNNCTG 8 cut(s) 329, 359, 386, 416, 446, 473, 500, 527
AoxI GGCC 7 cut(s) 285, 744, 892, 976, 1037, 1091, 1410
ApeKI GCWGC 4 cut(s) 329, 359, 386, 863
ApoI RAATTY 1 cut(s) 734
AsiGI ACCGGT 1 cut(s) 1324
Asp700I GAANNNNTTC 1 cut(s) 953
AspS9I GGNCC 2 cut(s) 744, 1091
AsuHPI GGTGA 4 cut(s) 79, 292, 772, 1276
BanI GGYRCC 1 cut(s) 574
BanII GRGCYC 4 cut(s) 204, 261, 860, 1140
BarI GAAGNNNNNNTAC 2 cut(s) 1325, 1357
BbsI GAAGAC 2 cut(s) 794, 956
Bbv12I GWGCWC 4 cut(s) 134, 204, 729, 860
BbvCI CCTCAGC 3 cut(s) 42, 1314, 1446
BbvI GCAGC 4 cut(s) 316, 346, 373, 850
BccI CCATC 2 cut(s) 1000, 1376
BceAI ACGGC 1 cut(s) 272
BciT130I CCWGG 4 cut(s) 20, 748, 1041, 1089
BciVI GTATCC 1 cut(s) 1028
BcoDI GTCTC 3 cut(s) 8, 1230, 1283
BcuI ACTAGT 1 cut(s) 1453
BfaI CTAG 1 cut(s) 1454
BfmI CTRYAG 1 cut(s) 794
BfuI GTATCC 1 cut(s) 1028
BglII AGATCT 1 cut(s) 840
BisI GCNGC 5 cut(s) 39, 330, 360, 387, 864
BlsI GCNGC 5 cut(s) 40, 331, 361, 388, 865
Bme1390I CCNGG 4 cut(s) 20, 748, 1041, 1089
BmgT120I GGNCC 2 cut(s) 744, 1091
BmiI GGNNCC 2 cut(s) 63, 576
BmrFI CCNGG 4 cut(s) 20, 748, 1041, 1089
BmsI GCATC 1 cut(s) 768
BpiI GAAGAC 2 cut(s) 794, 956
BplI GAGNNNNNCTC 4 cut(s) 830, 862, 1355, 1387
Bpu10I CCTNAGC 5 cut(s) 42, 1109, 1278, 1314, 1446
BpuEI CTTGAG 6 cut(s) 155, 760, 1006, 1061, 1283, 1391
BsaHI GRCGYC 1 cut(s) 297
BsaI GGTCTC 2 cut(s) 8, 1283
BsaJI CCNNGG 3 cut(s) 18, 747, 1088
BsaWI WCCGGW 2 cut(s) 613, 1324
Bsc4I CCNNNNNNNGG 4 cut(s) 25, 701, 1162, 1279
Bse118I RCCGGY 2 cut(s) 64, 1324
BseBI CCWGG 4 cut(s) 20, 748, 1041, 1089
BseDI CCNNGG 3 cut(s) 18, 747, 1088
BseLI CCNNNNNNNGG 4 cut(s) 25, 701, 1162, 1279
BseMII CTCAG 7 cut(s) 33, 579, 699, 845, 1305, 1386, 1437
BseRI GAGGAG 8 cut(s) 26, 74, 161, 287, 317, 851, 1331, 1363
BseXI GCAGC 4 cut(s) 316, 346, 373, 850
Bsh1236I CGCG 1 cut(s) 888
BshFI GGCC 7 cut(s) 287, 746, 894, 978, 1039, 1093, 1412
BshNI GGYRCC 1 cut(s) 574
BshTI ACCGGT 1 cut(s) 1324
BsiHKAI GWGCWC 4 cut(s) 134, 204, 729, 860
BsiSI CCGG 4 cut(s) 65, 614, 1325, 1413
BslI CCNNNNNNNGG 4 cut(s) 25, 701, 1162, 1279
BsmAI GTCTC 3 cut(s) 8, 1230, 1283
BsnI GGCC 7 cut(s) 287, 746, 894, 978, 1039, 1093, 1412
Bso31I GGTCTC 2 cut(s) 8, 1283
Bsp1286I GDGCHC 6 cut(s) 134, 204, 261, 729, 860, 1140
Bsp143I GATC 7 cut(s) 166, 704, 840, 850, 883, 1065, 1415
Bsp68I TCGCGA 1 cut(s) 888
BspACI CCGC 4 cut(s) 26, 32, 38, 1275
BspANI GGCC 7 cut(s) 287, 746, 894, 978, 1039, 1093, 1412
BspCNI CTCAG 7 cut(s) 34, 580, 700, 846, 1306, 1387, 1438
BspFNI CGCG 1 cut(s) 888
BspLI GGNNCC 2 cut(s) 63, 576
BspMAI CTGCAG 1 cut(s) 798
BspPI GGATC 3 cut(s) 712, 891, 1423
BspQI GCTCTTC 2 cut(s) 122, 930
BspT107I GGYRCC 1 cut(s) 574
BspTNI GGTCTC 2 cut(s) 8, 1283
BsrFI RCCGGY 2 cut(s) 64, 1324
BssAI RCCGGY 2 cut(s) 64, 1324
BssECI CCNNGG 3 cut(s) 18, 747, 1088
BssMI GATC 7 cut(s) 166, 704, 840, 850, 883, 1065, 1415
BssNI GRCGYC 1 cut(s) 297
Bst2UI CCWGG 4 cut(s) 20, 748, 1041, 1089
Bst4CI ACNGT 3 cut(s) 7, 121, 878
Bst6I CTCTTC 6 cut(s) 122, 309, 930, 960, 965, 1007
BstACI GRCGYC 1 cut(s) 297
BstAPI GCANNNNNTGC 1 cut(s) 1214
BstC8I GCNNGC 3 cut(s) 417, 1128, 1270
BstDEI CTNAG 9 cut(s) 42, 588, 708, 854, 1109, 1278, 1314, 1395, 1446
BstEII GGTNACC 1 cut(s) 1282
BstENI CCTNNNNNAGG 1 cut(s) 699
BstFNI CGCG 1 cut(s) 888
BstKTI GATC 7 cut(s) 169, 707, 843, 853, 886, 1068, 1418
BstMAI GTCTC 3 cut(s) 8, 1230, 1283
BstMBI GATC 7 cut(s) 166, 704, 840, 850, 883, 1065, 1415
BstMWI GCNNNNNNNGC 4 cut(s) 38, 368, 1214, 1274
BstNI CCWGG 4 cut(s) 20, 748, 1041, 1089
BstPI GGTNACC 1 cut(s) 1282
BstSCI CCNGG 4 cut(s) 18, 746, 1039, 1087
BstSFI CTRYAG 1 cut(s) 794
BstUI CGCG 1 cut(s) 888
BstV1I GCAGC 4 cut(s) 316, 346, 373, 850
BstV2I GAAGAC 2 cut(s) 794, 956
BstX2I RGATCY 2 cut(s) 704, 840
BstYI RGATCY 2 cut(s) 704, 840
BsuI GTATCC 1 cut(s) 1028
BsuRI GGCC 7 cut(s) 287, 746, 894, 978, 1039, 1093, 1412
BtsI GCAGTG 1 cut(s) 803
BtsIMutI CAGTG 2 cut(s) 117, 803
BtuMI TCGCGA 1 cut(s) 888
Cac8I GCNNGC 3 cut(s) 417, 1128, 1270
CaiI CAGNNNCTG 8 cut(s) 329, 359, 386, 416, 446, 473, 500, 527
Cfr10I RCCGGY 2 cut(s) 64, 1324
Cfr13I GGNCC 2 cut(s) 744, 1091
Csp6I GTAC 3 cut(s) 1201, 1303, 1451
CspAI ACCGGT 1 cut(s) 1324
CviQI GTAC 3 cut(s) 1201, 1303, 1451
DdeI CTNAG 9 cut(s) 42, 588, 708, 854, 1109, 1278, 1314, 1395, 1446
DpnI GATC 7 cut(s) 168, 706, 842, 852, 885, 1067, 1417
DpnII GATC 7 cut(s) 166, 704, 840, 850, 883, 1065, 1415
Eam1104I CTCTTC 6 cut(s) 122, 309, 930, 960, 965, 1007
EarI CTCTTC 6 cut(s) 122, 309, 930, 960, 965, 1007
Ecl136II GAGCTC 2 cut(s) 202, 858
Eco147I AGGCCT 1 cut(s) 1039
Eco24I GRGCYC 4 cut(s) 204, 261, 860, 1140
Eco31I GGTCTC 2 cut(s) 8, 1283
Eco32I GATATC 1 cut(s) 667
Eco53kI GAGCTC 2 cut(s) 202, 858
Eco57I CTGAAG 2 cut(s) 803, 1046
Eco91I GGTNACC 1 cut(s) 1282
EcoICRI GAGCTC 2 cut(s) 202, 858
EcoNI CCTNNNNNAGG 1 cut(s) 699
EcoO65I GGTNACC 1 cut(s) 1282
EcoRII CCWGG 4 cut(s) 18, 746, 1039, 1087
EcoRV GATATC 1 cut(s) 667
EcoT38I GRGCYC 4 cut(s) 204, 261, 860, 1140
FaiI YATR 6 cut(s) 141, 619, 904, 1206, 1335, 1430
FauI CCCGC 1 cut(s) 1282
FblI GTMKAC 1 cut(s) 657
Fnu4HI GCNGC 5 cut(s) 39, 330, 360, 387, 864
FriOI GRGCYC 4 cut(s) 204, 261, 860, 1140
Fsp4HI GCNGC 5 cut(s) 39, 330, 360, 387, 864
FspBI CTAG 1 cut(s) 1454
GluI GCNGC 5 cut(s) 39, 330, 360, 387, 864
HaeIII GGCC 7 cut(s) 287, 746, 894, 978, 1039, 1093, 1412
HapII CCGG 4 cut(s) 65, 614, 1325, 1413
Hin1I GRCGYC 1 cut(s) 297
HincII GTYRAC 1 cut(s) 658
HindII GTYRAC 1 cut(s) 658
HindIII AAGCTT 1 cut(s) 1025
HinfI GANTC 7 cut(s) 98, 104, 641, 790, 809, 821, 1237
HpaII CCGG 4 cut(s) 65, 614, 1325, 1413
HphI GGTGA 4 cut(s) 79, 292, 772, 1276
Hpy166II GTNNAC 2 cut(s) 658, 763
Hpy8I GTNNAC 2 cut(s) 658, 763
Hpy99I CGWCG 2 cut(s) 302, 659
HpyCH4III ACNGT 3 cut(s) 7, 121, 878
HpyCH4IV ACGT 2 cut(s) 297, 953
HpyCH4V TGCA 5 cut(s) 698, 796, 1118, 1144, 1208
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 368, 1214, 1274
HpyF3I CTNAG 9 cut(s) 42, 588, 708, 854, 1109, 1278, 1314, 1395, 1446
HpySE526I ACGT 2 cut(s) 297, 953
Hsp92I GRCGYC 1 cut(s) 297
Kzo9I GATC 7 cut(s) 166, 704, 840, 850, 883, 1065, 1415
LguI GCTCTTC 2 cut(s) 122, 930
LmnI GCTCC 2 cut(s) 61, 1105
Lsp1109I GCAGC 4 cut(s) 316, 346, 373, 850
LweI GCATC 1 cut(s) 768
MaeI CTAG 1 cut(s) 1454
MaeII ACGT 2 cut(s) 297, 953
MaeIII GTNAC 1 cut(s) 1282
MalI GATC 7 cut(s) 168, 706, 842, 852, 885, 1067, 1417
MboI GATC 7 cut(s) 166, 704, 840, 850, 883, 1065, 1415
MfeI CAATTG 1 cut(s) 1001
MflI RGATCY 2 cut(s) 704, 840
MhlI GDGCHC 6 cut(s) 134, 204, 261, 729, 860, 1140
MlyI GAGTC 4 cut(s) 113, 784, 818, 1231
MroXI GAANNNNTTC 1 cut(s) 953
MseI TTAA 1 cut(s) 1034
MspA1I CMGCKG 1 cut(s) 1313
MspI CCGG 4 cut(s) 65, 614, 1325, 1413
MspR9I CCNGG 4 cut(s) 20, 748, 1041, 1089
MunI CAATTG 1 cut(s) 1001
MvaI CCWGG 4 cut(s) 20, 748, 1041, 1089
MvnI CGCG 1 cut(s) 888
MwoI GCNNNNNNNGC 4 cut(s) 38, 368, 1214, 1274
NdeII GATC 7 cut(s) 166, 704, 840, 850, 883, 1065, 1415
NlaIV GGNNCC 2 cut(s) 63, 576
NmuCI GTSAC 1 cut(s) 1282
NruI TCGCGA 1 cut(s) 888
PceI AGGCCT 1 cut(s) 1039
PciSI GCTCTTC 2 cut(s) 122, 930
PcsI WCGNNNNNNNCGW 1 cut(s) 771
PdmI GAANNNNTTC 1 cut(s) 953
PfeI GAWTC 3 cut(s) 98, 641, 821
PinAI ACCGGT 1 cut(s) 1324
PkrI GCNGC 5 cut(s) 40, 331, 361, 388, 865
PleI GAGTC 4 cut(s) 112, 784, 817, 1231
PpsI GAGTC 4 cut(s) 112, 784, 817, 1231
PsiI TTATAA 1 cut(s) 141
Psp124BI GAGCTC 2 cut(s) 204, 860
Psp6I CCWGG 4 cut(s) 18, 746, 1039, 1087
PspEI GGTNACC 1 cut(s) 1282
PspGI CCWGG 4 cut(s) 18, 746, 1039, 1087
PspN4I GGNNCC 2 cut(s) 63, 576
PspPI GGNCC 2 cut(s) 744, 1091
PstI CTGCAG 1 cut(s) 798
PstNI CAGNNNCTG 8 cut(s) 329, 359, 386, 416, 446, 473, 500, 527
PsuI RGATCY 2 cut(s) 704, 840
PvuII CAGCTG 1 cut(s) 1313
RruI TCGCGA 1 cut(s) 888
RsaI GTAC 3 cut(s) 1202, 1304, 1452
RsaNI GTAC 3 cut(s) 1201, 1303, 1451
SacI GAGCTC 2 cut(s) 204, 860
SalI GTCGAC 1 cut(s) 656
SapI GCTCTTC 2 cut(s) 122, 930
SaqAI TTAA 1 cut(s) 1034
SatI GCNGC 5 cut(s) 39, 330, 360, 387, 864
Sau3AI GATC 7 cut(s) 166, 704, 840, 850, 883, 1065, 1415
Sau96I GGNCC 2 cut(s) 744, 1091
SchI GAGTC 4 cut(s) 113, 784, 818, 1231
ScrFI CCNGG 4 cut(s) 20, 748, 1041, 1089
SduI GDGCHC 6 cut(s) 134, 204, 261, 729, 860, 1140
SfaNI GCATC 1 cut(s) 768
SfcI CTRYAG 1 cut(s) 794
SmlI CTYRAG 6 cut(s) 170, 739, 985, 1076, 1298, 1406
SmoI CTYRAG 6 cut(s) 170, 739, 985, 1076, 1298, 1406
SpeI ACTAGT 1 cut(s) 1453
SseBI AGGCCT 1 cut(s) 1039
SsiI CCGC 4 cut(s) 26, 32, 38, 1275
SspI AATATT 1 cut(s) 1177
SspMI CTAG 1 cut(s) 1454
SstI GAGCTC 2 cut(s) 204, 860
StuI AGGCCT 1 cut(s) 1039
StyD4I CCNGG 4 cut(s) 18, 746, 1039, 1087
TaaI ACNGT 3 cut(s) 7, 121, 878
TaiI ACGT 2 cut(s) 300, 956
TaqI TCGA 6 cut(s) 209, 216, 300, 657, 774, 812
TatI WGTACW 1 cut(s) 1302
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 3 cut(s) 98, 641, 821
Tru1I TTAA 1 cut(s) 1034
Tru9I TTAA 1 cut(s) 1034
TscAI CASTG 2 cut(s) 124, 803
TseFI GTSAC 1 cut(s) 1282
TseI GCWGC 4 cut(s) 329, 359, 386, 863
Tsp45I GTSAC 1 cut(s) 1282
TspDTI ATGAA 2 cut(s) 978, 1171
TspRI CASTG 2 cut(s) 124, 803
XagI CCTNNNNNAGG 1 cut(s) 699
XapI RAATTY 1 cut(s) 734
XmiI GTMKAC 1 cut(s) 657
XmnI GAANNNNTTC 1 cut(s) 953
XspI CTAG 1 cut(s) 1454
ZraI GACGTC 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.