Rh5BG132400

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001644
Physical Location & Seq
Forward (+)
13037441 .. 13039852
2412 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG132400.1

Sequence Viewer

Length: 1161 bp
ATGACTGTGGAGGAGACCCAGGTGGCGGTGGCGGTGGCGGCTGAGGTCGTGGAGGTTGAGGAGCCGGTGAAGGAGGTGAAGGAGTGCGAGAAGGCTGTTGTTGAGGGTTTGGTGGAGGAATCCGAGTCCAAGCCAACCACTGTTGAGAAGAGCACTTCTTATAAGGAGGAGAGCAACTATCTCTCTGATCTCAAGGAGTTTGAGAAGAAGGCTTTGGTTGAGCTCAAATCGAAGCTCGAAGAAGCGATTTTGGGCAACAACATTTTCAAGAGAGATGAGCCCAAGAAGGTTGAGGAGAAGGAGAAGGCCGTAGCTGACGTGGAGGAGAAGAAGGAGGAGAAAACAGAGGCTGCTGTGAAAACAACGGAGAAAACAGAGGCTGCTGTGAAAGCAGAGGAAACAGAGCCTGCTGTGAAAGCAGAGGAAACAGAGGCTGTTGTGAAAACAGAGTGTGCTGAAAAAACAGAGGAAGGAGAAAAGGCACCAGAATTGGCTGAGAAGAAAGAAGAAGTAGCAGTTCCGGTATGCGAGGAAGAAGCGAAACCCGAATCCAGCGAAGTCGTCGACACAGATATCTCACTCTGGGGTGTGCCTCTGTTCCCTGGAAAAGGATCTGAGGGAACTGATGTGGTGCTCTTGAAATTCTTGAGGGCCAGGGAGTTCAAGGTGAACGAAGCGTTCGAGATGCTGAAGAAGACTCTGCAGTGGAGGAAGGAGTCGAAAATTGATTCAATTTTGGATGAGGAGATCTGCTCTGATCTGAGCTCTGCTGCTTACTTGAACGGCGTGGATCGCGAAGGCCACCCTGTTTGCTACAACATCTTCGGAGTTTTTGACAACGAAGAGCTTTACCAGAAGACGTTCGGAAATGAAGAGAAGAGAGGCCAATTCTTGAGGTGGAGGGTTCAATTAATGGAGAAGAGTATCCAGAAGCTTGAATTAAGGCCTGGTGGTGCTACTTCTCTGCTTCAGATCAATGACCTCAAGAACTCGCCTGGACCAATCAAGAAGGAGCTTAGGGTTGCAACAAAGCAAGCTGTTGGGCTCTTGCAGGACAATTACCCTGAATTGGTTGCCAAAAATGTAAGCTTTTTCGACTTTTCTAACATATTTACTTTACCAATTTTGATAGTTAGTTCTAAATTGATGGGTTTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

386

Amino Acids

43.28

Weight (kDa)

4.75

Isoelectric Point (pI)

51.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_N PF03765 182 - 232 2.7e-09 CRAL/TRIO, N-terminal domain
CRAL_TRIO PF00650 257 - 375 6.6e-08 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 162
AccB1I GGYRCC 1 cut(s) 481
AccI GTMKAC 1 cut(s) 564
AccII CGCG 1 cut(s) 795
AciI CCGC 3 cut(s) 26, 32, 38
AclWI GGATC 2 cut(s) 619, 798
AcsI RAATTY 1 cut(s) 641
AcuI CTGAAG 2 cut(s) 710, 953
AfiI CCNNNNNNNGG 3 cut(s) 25, 608, 1069
AgsI TTSAA 7 cut(s) 268, 640, 664, 732, 781, 908, 938
AjiI CACGTC 1 cut(s) 319
AjnI CCWGG 5 cut(s) 18, 601, 653, 946, 994
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
AluBI AGCT 9 cut(s) 223, 235, 314, 765, 847, 934, 1015, 1037, 1089
AluI AGCT 9 cut(s) 223, 235, 314, 765, 847, 934, 1015, 1037, 1089
Alw21I GWGCWC 4 cut(s) 155, 225, 636, 767
Alw26I GTCTC 1 cut(s) 8
AlwI GGATC 2 cut(s) 619, 798
AlwNI CAGNNNCTG 4 cut(s) 350, 380, 407, 434
AoxI GGCC 5 cut(s) 306, 651, 799, 883, 944
ApeKI GCWGC 3 cut(s) 350, 380, 770
ApoI RAATTY 1 cut(s) 641
AseI ATTAAT 1 cut(s) 911
Asp700I GAANNNNTTC 1 cut(s) 860
AspS9I GGNCC 2 cut(s) 651, 998
AsuHPI GGTGA 3 cut(s) 79, 88, 679
AvaII GGWCC 1 cut(s) 998
BanI GGYRCC 1 cut(s) 481
BanII GRGCYC 4 cut(s) 225, 282, 767, 1047
BbsI GAAGAC 2 cut(s) 701, 863
Bbv12I GWGCWC 4 cut(s) 155, 225, 636, 767
BbvCI CCTCAGC 1 cut(s) 42
BbvI GCAGC 3 cut(s) 337, 367, 757
BccI CCATC 1 cut(s) 1141
BceAI ACGGC 2 cut(s) 293, 799
BciT130I CCWGG 5 cut(s) 20, 603, 655, 948, 996
BciVI GTATCC 1 cut(s) 935
BcoDI GTCTC 1 cut(s) 8
BfmI CTRYAG 1 cut(s) 701
BfuI GTATCC 1 cut(s) 935
BglII AGATCT 1 cut(s) 747
BisI GCNGC 4 cut(s) 39, 351, 381, 771
BlsI GCNGC 4 cut(s) 40, 352, 382, 772
Bme1390I CCNGG 5 cut(s) 20, 603, 655, 948, 996
Bme18I GGWCC 1 cut(s) 998
BmgBI CACGTC 1 cut(s) 319
BmgT120I GGNCC 2 cut(s) 651, 998
BmiI GGNNCC 2 cut(s) 63, 483
BmrFI CCNGG 5 cut(s) 20, 603, 655, 948, 996
BmsI GCATC 1 cut(s) 675
BpiI GAAGAC 2 cut(s) 701, 863
BplI GAGNNNNNCTC 2 cut(s) 737, 769
Bpu10I CCTNAGC 2 cut(s) 42, 1016
BpuEI CTTGAG 4 cut(s) 176, 667, 913, 968
BsaI GGTCTC 1 cut(s) 8
BsaJI CCNNGG 3 cut(s) 18, 601, 654
BsaWI WCCGGW 1 cut(s) 520
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 608, 1069
Bse118I RCCGGY 1 cut(s) 64
BseBI CCWGG 5 cut(s) 20, 603, 655, 948, 996
BseDI CCNNGG 3 cut(s) 18, 601, 654
BseGI GGATG 1 cut(s) 745
BseLI CCNNNNNNNGG 3 cut(s) 25, 608, 1069
BseMII CTCAG 4 cut(s) 33, 486, 606, 752
BseRI GAGGAG 7 cut(s) 26, 74, 182, 308, 338, 350, 758
BseXI GCAGC 3 cut(s) 337, 367, 757
Bsh1236I CGCG 1 cut(s) 795
BshFI GGCC 5 cut(s) 308, 653, 801, 885, 946
BshNI GGYRCC 1 cut(s) 481
BsiHKAI GWGCWC 4 cut(s) 155, 225, 636, 767
BsiSI CCGG 2 cut(s) 65, 521
BslI CCNNNNNNNGG 3 cut(s) 25, 608, 1069
BsmAI GTCTC 1 cut(s) 8
BsnI GGCC 5 cut(s) 308, 653, 801, 885, 946
Bso31I GGTCTC 1 cut(s) 8
Bsp1286I GDGCHC 6 cut(s) 155, 225, 282, 636, 767, 1047
Bsp143I GATC 6 cut(s) 187, 611, 747, 757, 790, 972
Bsp68I TCGCGA 1 cut(s) 795
BspACI CCGC 3 cut(s) 26, 32, 38
BspANI GGCC 5 cut(s) 308, 653, 801, 885, 946
BspCNI CTCAG 4 cut(s) 34, 487, 607, 753
BspFNI CGCG 1 cut(s) 795
BspLI GGNNCC 2 cut(s) 63, 483
BspMAI CTGCAG 1 cut(s) 705
BspPI GGATC 2 cut(s) 619, 798
BspQI GCTCTTC 2 cut(s) 143, 837
BspT107I GGYRCC 1 cut(s) 481
BspTNI GGTCTC 1 cut(s) 8
BsrFI RCCGGY 1 cut(s) 64
BssAI RCCGGY 1 cut(s) 64
BssECI CCNNGG 3 cut(s) 18, 601, 654
BssMI GATC 6 cut(s) 187, 611, 747, 757, 790, 972
Bst2UI CCWGG 5 cut(s) 20, 603, 655, 948, 996
Bst4CI ACNGT 2 cut(s) 7, 142
Bst6I CTCTTC 5 cut(s) 143, 837, 867, 872, 914
BstC8I GCNNGC 2 cut(s) 408, 1035
BstDEI CTNAG 5 cut(s) 42, 495, 615, 761, 1016
BstENI CCTNNNNNAGG 1 cut(s) 606
BstF5I GGATG 1 cut(s) 745
BstFNI CGCG 1 cut(s) 795
BstKTI GATC 6 cut(s) 190, 614, 750, 760, 793, 975
BstMAI GTCTC 1 cut(s) 8
BstMBI GATC 6 cut(s) 187, 611, 747, 757, 790, 972
BstMWI GCNNNNNNNGC 4 cut(s) 38, 389, 416, 792
BstNI CCWGG 5 cut(s) 20, 603, 655, 948, 996
BstSCI CCNGG 5 cut(s) 18, 601, 653, 946, 994
BstSFI CTRYAG 1 cut(s) 701
BstUI CGCG 1 cut(s) 795
BstV1I GCAGC 3 cut(s) 337, 367, 757
BstV2I GAAGAC 2 cut(s) 701, 863
BstX2I RGATCY 2 cut(s) 611, 747
BstYI RGATCY 2 cut(s) 611, 747
BsuI GTATCC 1 cut(s) 935
BsuRI GGCC 5 cut(s) 308, 653, 801, 885, 946
BtrI CACGTC 1 cut(s) 319
BtsCI GGATG 1 cut(s) 745
BtsI GCAGTG 1 cut(s) 710
BtsIMutI CAGTG 2 cut(s) 138, 710
BtuMI TCGCGA 1 cut(s) 795
Cac8I GCNNGC 2 cut(s) 408, 1035
CaiI CAGNNNCTG 4 cut(s) 350, 380, 407, 434
Cfr10I RCCGGY 1 cut(s) 64
Cfr13I GGNCC 2 cut(s) 651, 998
CspCI CAANNNNNGTGG 2 cut(s) 791, 826
DdeI CTNAG 5 cut(s) 42, 495, 615, 761, 1016
DpnI GATC 6 cut(s) 189, 613, 749, 759, 792, 974
DpnII GATC 6 cut(s) 187, 611, 747, 757, 790, 972
Eam1104I CTCTTC 5 cut(s) 143, 837, 867, 872, 914
EarI CTCTTC 5 cut(s) 143, 837, 867, 872, 914
Ecl136II GAGCTC 2 cut(s) 223, 765
Eco147I AGGCCT 1 cut(s) 946
Eco24I GRGCYC 4 cut(s) 225, 282, 767, 1047
Eco31I GGTCTC 1 cut(s) 8
Eco32I GATATC 1 cut(s) 574
Eco47I GGWCC 1 cut(s) 998
Eco53kI GAGCTC 2 cut(s) 223, 765
Eco57I CTGAAG 2 cut(s) 710, 953
EcoICRI GAGCTC 2 cut(s) 223, 765
EcoNI CCTNNNNNAGG 1 cut(s) 606
EcoRII CCWGG 5 cut(s) 18, 601, 653, 946, 994
EcoRV GATATC 1 cut(s) 574
EcoT38I GRGCYC 4 cut(s) 225, 282, 767, 1047
FaiI YATR 3 cut(s) 162, 526, 1109
FblI GTMKAC 1 cut(s) 564
Fnu4HI GCNGC 4 cut(s) 39, 351, 381, 771
FokI GGATG 1 cut(s) 752
FriOI GRGCYC 4 cut(s) 225, 282, 767, 1047
Fsp4HI GCNGC 4 cut(s) 39, 351, 381, 771
GluI GCNGC 4 cut(s) 39, 351, 381, 771
HaeIII GGCC 5 cut(s) 308, 653, 801, 885, 946
HapII CCGG 2 cut(s) 65, 521
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HindIII AAGCTT 2 cut(s) 932, 1087
HinfI GANTC 6 cut(s) 119, 125, 548, 697, 716, 728
HpaII CCGG 2 cut(s) 65, 521
HphI GGTGA 3 cut(s) 79, 88, 679
Hpy166II GTNNAC 2 cut(s) 565, 670
Hpy188I TCNGA 8 cut(s) 124, 187, 616, 757, 762, 827, 866, 972
Hpy188III TCNNGA 9 cut(s) 268, 637, 646, 682, 794, 892, 928, 985, 1006
Hpy8I GTNNAC 2 cut(s) 565, 670
Hpy99I CGWCG 1 cut(s) 566
HpyCH4III ACNGT 2 cut(s) 7, 142
HpyCH4IV ACGT 2 cut(s) 318, 860
HpyCH4V TGCA 3 cut(s) 703, 1025, 1051
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 389, 416, 792
HpyF3I CTNAG 5 cut(s) 42, 495, 615, 761, 1016
HpySE526I ACGT 2 cut(s) 318, 860
Kzo9I GATC 6 cut(s) 187, 611, 747, 757, 790, 972
LguI GCTCTTC 2 cut(s) 143, 837
LmnI GCTCC 2 cut(s) 61, 1012
Lsp1109I GCAGC 3 cut(s) 337, 367, 757
LweI GCATC 1 cut(s) 675
MaeII ACGT 2 cut(s) 318, 860
MalI GATC 6 cut(s) 189, 613, 749, 759, 792, 974
MboI GATC 6 cut(s) 187, 611, 747, 757, 790, 972
MflI RGATCY 2 cut(s) 611, 747
MhlI GDGCHC 6 cut(s) 155, 225, 282, 636, 767, 1047
MlyI GAGTC 3 cut(s) 134, 691, 725
MroXI GAANNNNTTC 1 cut(s) 860
MseI TTAA 3 cut(s) 911, 941, 1159
MspI CCGG 2 cut(s) 65, 521
MspR9I CCNGG 5 cut(s) 20, 603, 655, 948, 996
MvaI CCWGG 5 cut(s) 20, 603, 655, 948, 996
MvnI CGCG 1 cut(s) 795
MwoI GCNNNNNNNGC 4 cut(s) 38, 389, 416, 792
NdeII GATC 6 cut(s) 187, 611, 747, 757, 790, 972
NlaIV GGNNCC 2 cut(s) 63, 483
NruI TCGCGA 1 cut(s) 795
PceI AGGCCT 1 cut(s) 946
PciSI GCTCTTC 2 cut(s) 143, 837
PcsI WCGNNNNNNNCGW 1 cut(s) 678
PdmI GAANNNNTTC 1 cut(s) 860
PfeI GAWTC 3 cut(s) 119, 548, 728
PkrI GCNGC 4 cut(s) 40, 352, 382, 772
PleI GAGTC 3 cut(s) 133, 691, 724
PpsI GAGTC 3 cut(s) 133, 691, 724
PshBI ATTAAT 1 cut(s) 911
PsiI TTATAA 1 cut(s) 162
Psp124BI GAGCTC 2 cut(s) 225, 767
Psp6I CCWGG 5 cut(s) 18, 601, 653, 946, 994
PspGI CCWGG 5 cut(s) 18, 601, 653, 946, 994
PspN4I GGNNCC 2 cut(s) 63, 483
PspPI GGNCC 2 cut(s) 651, 998
PstI CTGCAG 1 cut(s) 705
PstNI CAGNNNCTG 4 cut(s) 350, 380, 407, 434
PsuI RGATCY 2 cut(s) 611, 747
RruI TCGCGA 1 cut(s) 795
SacI GAGCTC 2 cut(s) 225, 767
SalI GTCGAC 1 cut(s) 563
SapI GCTCTTC 2 cut(s) 143, 837
SaqAI TTAA 3 cut(s) 911, 941, 1159
SatI GCNGC 4 cut(s) 39, 351, 381, 771
Sau3AI GATC 6 cut(s) 187, 611, 747, 757, 790, 972
Sau96I GGNCC 2 cut(s) 651, 998
SchI GAGTC 3 cut(s) 134, 691, 725
ScrFI CCNGG 5 cut(s) 20, 603, 655, 948, 996
SduI GDGCHC 6 cut(s) 155, 225, 282, 636, 767, 1047
SfaNI GCATC 1 cut(s) 675
SfcI CTRYAG 1 cut(s) 701
SinI GGWCC 1 cut(s) 998
SmlI CTYRAG 4 cut(s) 191, 646, 892, 983
SmoI CTYRAG 4 cut(s) 191, 646, 892, 983
SseBI AGGCCT 1 cut(s) 946
SsiI CCGC 3 cut(s) 26, 32, 38
SstI GAGCTC 2 cut(s) 225, 767
StuI AGGCCT 1 cut(s) 946
StyD4I CCNGG 5 cut(s) 18, 601, 653, 946, 994
TaaI ACNGT 2 cut(s) 7, 142
TaiI ACGT 2 cut(s) 321, 863
TaqI TCGA 6 cut(s) 230, 237, 564, 681, 719, 1095
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 3 cut(s) 119, 548, 728
Tru1I TTAA 3 cut(s) 911, 941, 1159
Tru9I TTAA 3 cut(s) 911, 941, 1159
TscAI CASTG 2 cut(s) 145, 710
TseI GCWGC 3 cut(s) 350, 380, 770
TspDTI ATGAA 1 cut(s) 885
TspGWI ACGGA 1 cut(s) 380
TspRI CASTG 2 cut(s) 145, 710
VpaK11BI GGWCC 1 cut(s) 998
VspI ATTAAT 1 cut(s) 911
XagI CCTNNNNNAGG 1 cut(s) 606
XapI RAATTY 1 cut(s) 641
XmiI GTMKAC 1 cut(s) 564
XmnI GAANNNNTTC 1 cut(s) 860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.