Rh5AG133900

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001644
Physical Location & Seq
Forward (+)
13633741 .. 13636411
2671 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG133900.1

Sequence Viewer

Length: 1245 bp
ATGACTGTGGAGGAGACCCAGGTGGCGGCGGCTGAGGTCGTGGAGGTTGAGGAGCCGGTGAAGGAGGTGAAGGAGTGCGAGAAGGCTGTTGTTGAGGAATCCGAGTCCAAGCCAACCACTGTTGAGAAGAGCACTTCTTATAAGGAGGAGAGCAACTATCTCTCTGATCTCAAGGAGTTTGAGAAGAAGGCTTTGGTTGAGCTCAAATATAAGCTCGAAGAAGCCATTTTGGGCAACAACATTTTCAAGAAAGAAGAGCCCAAGAAGGTTGAGGAGAAGGTGAAGGCGGTAGCTGAGGTCGAGGAGAAGAAGGAAGAGAAAACAGAGGCTGCTGTGAAAACAACAGAGGAAACAGAGGCTGCTGTGAAAGCAGAGGAAACAGAGCCTGCTGTGAAATCAGAGGAAACAGAGCCTGTTGTGAAATCAGAGGAAACAGAGCCTGCTGTTAAAGCAGAGGCAACAGAGGCTGTTGTGAAAACAGAGTGTGCTGAAAAAACAGAGGAAGGAGAAAAGGCACCAGAATTGGCTGAGAAGAAAGAAGAAGTAGCAGTTCCGGTATGCGAGGAAGAAGCGAAACCCGAATCCAGCGAAGTCGTCGACACAGATATCTCACTCTGGGGTGTGCCTCTGTTCCCTGGAAAAGGATCTGAGGGAACAGATGTGGTGCTCTTGAAATTCTTGAGGGCCAGGGAGTTCAAGGTGAACGAAGCCTTCGAGATGTTGAAGAAGACTCTGCAGTGGAGGAAGGAGTCGAAAATTGATTCAATTTTGGGTGAGGAGATCTGCTCTGATCTGAGCTCTGCTGCGTACTTGAACGGCGTGGATCGCGAAGGCCACCCTGTTTGCTACAACATCTTCGGAGTTTTTGACAACGAAGAGCTTTACCAGAAGACGTTCGGAAATGAAGAGAAGAGAGGCCAATTCTTGAGGTGGAGGGTTCAATTGATGGAGAAGAGTATCCAGAAGCTTGAATTAAGGCCTGGTGGTGCTACTTCTCTGCTTCAGATCAATGACCTCAAGAACTCGCCTGGGCCAATCAAGAAGGAGCTTAGGGTTGCAACAAAGCAAGCTGTTGGGCTCTTGCAGGACAATTACCCTGAATTGGTTGCCAAAAATATTTTCATCAATGTTCCATTCTGGTACTATGCACTCAATGCTCTGTTGTCTCCATTCTTGACTCAAAGAACCAAGAGCAAGTTTGTGGTGGCTCGCCCCGCTAAGGTCACCGAGACCCTTCTCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

414

Amino Acids

46.58

Weight (kDa)

4.87

Isoelectric Point (pI)

56.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_N PF03765 193 - 243 3.1e-09 CRAL/TRIO, N-terminal domain
CRAL_TRIO PF00650 268 - 413 2.1e-17 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 141
AccB1I GGYRCC 1 cut(s) 514
AccI GTMKAC 1 cut(s) 597
AccII CGCG 1 cut(s) 828
AciI CCGC 4 cut(s) 26, 29, 287, 1215
AclWI GGATC 2 cut(s) 652, 831
AcsI RAATTY 1 cut(s) 674
AcuI CTGAAG 1 cut(s) 986
AfaI GTAC 2 cut(s) 809, 1142
AfiI CCNNNNNNNGG 4 cut(s) 25, 641, 1102, 1219
AgsI TTSAA 8 cut(s) 247, 673, 697, 724, 765, 814, 941, 971
AjnI CCWGG 5 cut(s) 18, 634, 686, 979, 1027
AjuI GAANNNNNNNTTGG 4 cut(s) 176, 208, 1103, 1135
AluBI AGCT 8 cut(s) 202, 214, 293, 798, 880, 967, 1048, 1070
AluI AGCT 8 cut(s) 202, 214, 293, 798, 880, 967, 1048, 1070
Alw21I GWGCWC 4 cut(s) 134, 204, 669, 800
Alw26I GTCTC 3 cut(s) 8, 1170, 1223
AlwI GGATC 2 cut(s) 652, 831
AlwNI CAGNNNCTG 6 cut(s) 329, 359, 386, 413, 440, 467
AoxI GGCC 5 cut(s) 684, 832, 916, 977, 1031
ApeKI GCWGC 3 cut(s) 329, 359, 803
ApoI RAATTY 1 cut(s) 674
Asp700I GAANNNNTTC 1 cut(s) 893
AspS9I GGNCC 2 cut(s) 684, 1031
AsuHPI GGTGA 6 cut(s) 70, 79, 292, 712, 785, 1216
BanI GGYRCC 1 cut(s) 514
BanII GRGCYC 4 cut(s) 204, 261, 800, 1080
BbsI GAAGAC 2 cut(s) 734, 896
Bbv12I GWGCWC 4 cut(s) 134, 204, 669, 800
BbvCI CCTCAGC 2 cut(s) 33, 294
BbvI GCAGC 3 cut(s) 316, 346, 790
BccI CCATC 1 cut(s) 940
BceAI ACGGC 1 cut(s) 832
BciT130I CCWGG 5 cut(s) 20, 636, 688, 981, 1029
BciVI GTATCC 1 cut(s) 968
BcoDI GTCTC 3 cut(s) 8, 1170, 1223
BfmI CTRYAG 1 cut(s) 734
BfuI GTATCC 1 cut(s) 968
BglII AGATCT 1 cut(s) 780
BisI GCNGC 5 cut(s) 27, 30, 330, 360, 804
BlsI GCNGC 5 cut(s) 28, 31, 331, 361, 805
Bme1390I CCNGG 5 cut(s) 20, 636, 688, 981, 1029
BmgT120I GGNCC 2 cut(s) 684, 1031
BmiI GGNNCC 2 cut(s) 54, 516
BmrFI CCNGG 5 cut(s) 20, 636, 688, 981, 1029
BpiI GAAGAC 2 cut(s) 734, 896
BplI GAGNNNNNCTC 2 cut(s) 770, 802
Bpu10I CCTNAGC 4 cut(s) 33, 294, 1049, 1218
BpuEI CTTGAG 5 cut(s) 155, 700, 946, 1001, 1223
BsaI GGTCTC 2 cut(s) 8, 1223
BsaJI CCNNGG 4 cut(s) 18, 634, 687, 1028
BsaWI WCCGGW 1 cut(s) 553
Bsc4I CCNNNNNNNGG 4 cut(s) 25, 641, 1102, 1219
Bse118I RCCGGY 1 cut(s) 55
BseBI CCWGG 5 cut(s) 20, 636, 688, 981, 1029
BseDI CCNNGG 4 cut(s) 18, 634, 687, 1028
BseLI CCNNNNNNNGG 4 cut(s) 25, 641, 1102, 1219
BseMII CTCAG 5 cut(s) 24, 285, 519, 639, 785
BseRI GAGGAG 6 cut(s) 26, 65, 161, 287, 317, 791
BseXI GCAGC 3 cut(s) 316, 346, 790
Bsh1236I CGCG 1 cut(s) 828
BshFI GGCC 5 cut(s) 686, 834, 918, 979, 1033
BshNI GGYRCC 1 cut(s) 514
BsiHKAI GWGCWC 4 cut(s) 134, 204, 669, 800
BsiSI CCGG 2 cut(s) 56, 554
BslI CCNNNNNNNGG 4 cut(s) 25, 641, 1102, 1219
BsmAI GTCTC 3 cut(s) 8, 1170, 1223
BsnI GGCC 5 cut(s) 686, 834, 918, 979, 1033
Bso31I GGTCTC 2 cut(s) 8, 1223
Bsp1286I GDGCHC 6 cut(s) 134, 204, 261, 669, 800, 1080
Bsp143I GATC 6 cut(s) 166, 644, 780, 790, 823, 1005
Bsp68I TCGCGA 1 cut(s) 828
BspACI CCGC 4 cut(s) 26, 29, 287, 1215
BspANI GGCC 5 cut(s) 686, 834, 918, 979, 1033
BspCNI CTCAG 5 cut(s) 25, 286, 520, 640, 786
BspFNI CGCG 1 cut(s) 828
BspLI GGNNCC 2 cut(s) 54, 516
BspMAI CTGCAG 1 cut(s) 738
BspPI GGATC 2 cut(s) 652, 831
BspQI GCTCTTC 3 cut(s) 122, 249, 870
BspT107I GGYRCC 1 cut(s) 514
BspTNI GGTCTC 2 cut(s) 8, 1223
BsrFI RCCGGY 1 cut(s) 55
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 4 cut(s) 18, 634, 687, 1028
BssMI GATC 6 cut(s) 166, 644, 780, 790, 823, 1005
Bst2UI CCWGG 5 cut(s) 20, 636, 688, 981, 1029
Bst4CI ACNGT 2 cut(s) 7, 121
Bst6I CTCTTC 7 cut(s) 122, 249, 309, 870, 900, 905, 947
BstAPI GCANNNNNTGC 1 cut(s) 1154
BstC8I GCNNGC 4 cut(s) 387, 441, 1068, 1210
BstDEI CTNAG 7 cut(s) 33, 294, 528, 648, 794, 1049, 1218
BstEII GGTNACC 1 cut(s) 1222
BstENI CCTNNNNNAGG 1 cut(s) 639
BstFNI CGCG 1 cut(s) 828
BstKTI GATC 6 cut(s) 169, 647, 783, 793, 826, 1008
BstMAI GTCTC 3 cut(s) 8, 1170, 1223
BstMBI GATC 6 cut(s) 166, 644, 780, 790, 823, 1005
BstMWI GCNNNNNNNGC 6 cut(s) 368, 449, 464, 825, 1154, 1214
BstNI CCWGG 5 cut(s) 20, 636, 688, 981, 1029
BstPI GGTNACC 1 cut(s) 1222
BstSCI CCNGG 5 cut(s) 18, 634, 686, 979, 1027
BstSFI CTRYAG 1 cut(s) 734
BstUI CGCG 1 cut(s) 828
BstV1I GCAGC 3 cut(s) 316, 346, 790
BstV2I GAAGAC 2 cut(s) 734, 896
BstX2I RGATCY 2 cut(s) 644, 780
BstYI RGATCY 2 cut(s) 644, 780
BsuI GTATCC 1 cut(s) 968
BsuRI GGCC 5 cut(s) 686, 834, 918, 979, 1033
BtsI GCAGTG 1 cut(s) 743
BtsIMutI CAGTG 2 cut(s) 117, 743
BtuMI TCGCGA 1 cut(s) 828
Cac8I GCNNGC 4 cut(s) 387, 441, 1068, 1210
CaiI CAGNNNCTG 6 cut(s) 329, 359, 386, 413, 440, 467
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 2 cut(s) 684, 1031
Csp6I GTAC 2 cut(s) 808, 1141
CspCI CAANNNNNGTGG 2 cut(s) 824, 859
CviQI GTAC 2 cut(s) 808, 1141
DdeI CTNAG 7 cut(s) 33, 294, 528, 648, 794, 1049, 1218
DpnI GATC 6 cut(s) 168, 646, 782, 792, 825, 1007
DpnII GATC 6 cut(s) 166, 644, 780, 790, 823, 1005
Eam1104I CTCTTC 7 cut(s) 122, 249, 309, 870, 900, 905, 947
EarI CTCTTC 7 cut(s) 122, 249, 309, 870, 900, 905, 947
Ecl136II GAGCTC 2 cut(s) 202, 798
Eco147I AGGCCT 1 cut(s) 979
Eco24I GRGCYC 4 cut(s) 204, 261, 800, 1080
Eco31I GGTCTC 2 cut(s) 8, 1223
Eco32I GATATC 1 cut(s) 607
Eco53kI GAGCTC 2 cut(s) 202, 798
Eco57I CTGAAG 1 cut(s) 986
Eco91I GGTNACC 1 cut(s) 1222
EcoICRI GAGCTC 2 cut(s) 202, 798
EcoNI CCTNNNNNAGG 1 cut(s) 639
EcoO65I GGTNACC 1 cut(s) 1222
EcoRII CCWGG 5 cut(s) 18, 634, 686, 979, 1027
EcoRV GATATC 1 cut(s) 607
EcoT38I GRGCYC 4 cut(s) 204, 261, 800, 1080
FaiI YATR 4 cut(s) 141, 210, 559, 1146
FauI CCCGC 1 cut(s) 1222
FblI GTMKAC 1 cut(s) 597
Fnu4HI GCNGC 5 cut(s) 27, 30, 330, 360, 804
FriOI GRGCYC 4 cut(s) 204, 261, 800, 1080
Fsp4HI GCNGC 5 cut(s) 27, 30, 330, 360, 804
GluI GCNGC 5 cut(s) 27, 30, 330, 360, 804
HaeIII GGCC 5 cut(s) 686, 834, 918, 979, 1033
HapII CCGG 2 cut(s) 56, 554
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HindIII AAGCTT 1 cut(s) 965
HinfI GANTC 7 cut(s) 98, 104, 581, 730, 749, 761, 1177
HpaII CCGG 2 cut(s) 56, 554
HphI GGTGA 6 cut(s) 70, 79, 292, 712, 785, 1216
Hpy166II GTNNAC 2 cut(s) 598, 703
Hpy8I GTNNAC 2 cut(s) 598, 703
Hpy99I CGWCG 1 cut(s) 599
HpyCH4III ACNGT 2 cut(s) 7, 121
HpyCH4IV ACGT 1 cut(s) 893
HpyCH4V TGCA 4 cut(s) 736, 1058, 1084, 1148
HpyF10VI GCNNNNNNNGC 6 cut(s) 368, 449, 464, 825, 1154, 1214
HpyF3I CTNAG 7 cut(s) 33, 294, 528, 648, 794, 1049, 1218
HpySE526I ACGT 1 cut(s) 893
Kzo9I GATC 6 cut(s) 166, 644, 780, 790, 823, 1005
LguI GCTCTTC 3 cut(s) 122, 249, 870
LmnI GCTCC 2 cut(s) 52, 1045
Lsp1109I GCAGC 3 cut(s) 316, 346, 790
MaeII ACGT 1 cut(s) 893
MaeIII GTNAC 1 cut(s) 1222
MalI GATC 6 cut(s) 168, 646, 782, 792, 825, 1007
MboI GATC 6 cut(s) 166, 644, 780, 790, 823, 1005
MfeI CAATTG 1 cut(s) 941
MflI RGATCY 2 cut(s) 644, 780
MhlI GDGCHC 6 cut(s) 134, 204, 261, 669, 800, 1080
MluCI AATT 9 cut(s) 521, 674, 756, 765, 920, 941, 971, 1090, 1100
MlyI GAGTC 4 cut(s) 113, 724, 758, 1171
MroXI GAANNNNTTC 1 cut(s) 893
MseI TTAA 2 cut(s) 447, 974
MspI CCGG 2 cut(s) 56, 554
MspR9I CCNGG 5 cut(s) 20, 636, 688, 981, 1029
MunI CAATTG 1 cut(s) 941
MvaI CCWGG 5 cut(s) 20, 636, 688, 981, 1029
MvnI CGCG 1 cut(s) 828
MwoI GCNNNNNNNGC 6 cut(s) 368, 449, 464, 825, 1154, 1214
NdeII GATC 6 cut(s) 166, 644, 780, 790, 823, 1005
NlaIV GGNNCC 2 cut(s) 54, 516
NmuCI GTSAC 1 cut(s) 1222
NruI TCGCGA 1 cut(s) 828
PceI AGGCCT 1 cut(s) 979
PciSI GCTCTTC 3 cut(s) 122, 249, 870
PcsI WCGNNNNNNNCGW 1 cut(s) 711
PdmI GAANNNNTTC 1 cut(s) 893
PfeI GAWTC 3 cut(s) 98, 581, 761
PkrI GCNGC 5 cut(s) 28, 31, 331, 361, 805
PleI GAGTC 4 cut(s) 112, 724, 757, 1171
PpsI GAGTC 4 cut(s) 112, 724, 757, 1171
PsiI TTATAA 1 cut(s) 141
Psp124BI GAGCTC 2 cut(s) 204, 800
Psp6I CCWGG 5 cut(s) 18, 634, 686, 979, 1027
PspEI GGTNACC 1 cut(s) 1222
PspGI CCWGG 5 cut(s) 18, 634, 686, 979, 1027
PspN4I GGNNCC 2 cut(s) 54, 516
PspPI GGNCC 2 cut(s) 684, 1031
PstI CTGCAG 1 cut(s) 738
PstNI CAGNNNCTG 6 cut(s) 329, 359, 386, 413, 440, 467
PsuI RGATCY 2 cut(s) 644, 780
RruI TCGCGA 1 cut(s) 828
RsaI GTAC 2 cut(s) 809, 1142
RsaNI GTAC 2 cut(s) 808, 1141
SacI GAGCTC 2 cut(s) 204, 800
SalI GTCGAC 1 cut(s) 596
SapI GCTCTTC 3 cut(s) 122, 249, 870
SaqAI TTAA 2 cut(s) 447, 974
SatI GCNGC 5 cut(s) 27, 30, 330, 360, 804
Sau3AI GATC 6 cut(s) 166, 644, 780, 790, 823, 1005
Sau96I GGNCC 2 cut(s) 684, 1031
SchI GAGTC 4 cut(s) 113, 724, 758, 1171
ScrFI CCNGG 5 cut(s) 20, 636, 688, 981, 1029
SduI GDGCHC 6 cut(s) 134, 204, 261, 669, 800, 1080
SfcI CTRYAG 1 cut(s) 734
SmlI CTYRAG 5 cut(s) 170, 679, 925, 1016, 1238
SmoI CTYRAG 5 cut(s) 170, 679, 925, 1016, 1238
Sse9I AATT 9 cut(s) 521, 674, 756, 765, 920, 941, 971, 1090, 1100
SseBI AGGCCT 1 cut(s) 979
SsiI CCGC 4 cut(s) 26, 29, 287, 1215
SspI AATATT 1 cut(s) 1117
SstI GAGCTC 2 cut(s) 204, 800
StuI AGGCCT 1 cut(s) 979
StyD4I CCNGG 5 cut(s) 18, 634, 686, 979, 1027
TaaI ACNGT 2 cut(s) 7, 121
TaiI ACGT 1 cut(s) 896
TaqI TCGA 5 cut(s) 216, 300, 597, 714, 752
TasI AATT 9 cut(s) 521, 674, 756, 765, 920, 941, 971, 1090, 1100
TauI GCSGC 2 cut(s) 29, 32
TfiI GAWTC 3 cut(s) 98, 581, 761
Tru1I TTAA 2 cut(s) 447, 974
Tru9I TTAA 2 cut(s) 447, 974
TscAI CASTG 2 cut(s) 124, 743
TseFI GTSAC 1 cut(s) 1222
TseI GCWGC 3 cut(s) 329, 359, 803
Tsp45I GTSAC 1 cut(s) 1222
TspDTI ATGAA 2 cut(s) 918, 1111
TspRI CASTG 2 cut(s) 124, 743
XagI CCTNNNNNAGG 1 cut(s) 639
XapI RAATTY 1 cut(s) 674
XmiI GTMKAC 1 cut(s) 597
XmnI GAANNNNTTC 1 cut(s) 893
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.