CRAL/TRIO, N-terminal domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1799 |
| AarI | CACCTGC | 1 cut(s) | 1930 |
| AatII | GACGTC | 1 cut(s) | 2112 |
| Acc36I | ACCTGC | 1 cut(s) | 1930 |
| AccI | GTMKAC | 1 cut(s) | 2242 |
| AccII | CGCG | 1 cut(s) | 2387 |
| AciI | CCGC | 2 cut(s) | 731, 1145 |
| AclWI | GGATC | 6 cut(s) | 44, 1215, 1797, 2171, 2353, 2387 |
| AcsI | RAATTY | 4 cut(s) | 1547, 2075, 2324, 2481 |
| AcuI | CTGAAG | 6 cut(s) | 14, 75, 552, 627, 1797, 2008 |
| AcyI | GRCGYC | 1 cut(s) | 2109 |
| AfaI | GTAC | 8 cut(s) | 289, 499, 832, 1497, 1767, 2015, 2123, 2364 |
| AfeI | AGCGCT | 1 cut(s) | 2035 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 1611, 2167, 2264, 2338 |
| AflIII | ACRYGT | 1 cut(s) | 1791 |
| AjnI | CCWGG | 2 cut(s) | 1852, 1900 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1124, 1156, 2060, 2092 |
| Alw26I | GTCTC | 2 cut(s) | 1301, 2209 |
| AlwI | GGATC | 6 cut(s) | 44, 1215, 1797, 2171, 2353, 2387 |
| Aor51HI | AGCGCT | 1 cut(s) | 2035 |
| AoxI | GGCC | 6 cut(s) | 270, 340, 550, 882, 2090, 2158 |
| ApeKI | GCWGC | 3 cut(s) | 113, 1391, 1735 |
| ApoI | RAATTY | 4 cut(s) | 1547, 2075, 2324, 2481 |
| ArsI | GACNNNNNNTTYG | 8 cut(s) | 1637, 1669, 1698, 1730, 1888, 1920, 2187, 2219 |
| AspLEI | GCGC | 3 cut(s) | 2036, 2387, 2389 |
| AspS9I | GGNCC | 4 cut(s) | 340, 550, 883, 1904 |
| AsuC2I | CCSGG | 2 cut(s) | 1712, 2430 |
| AsuHPI | GGTGA | 5 cut(s) | 385, 595, 928, 1670, 2037 |
| AsuII | TTCGAA | 1 cut(s) | 1924 |
| AsuNHI | GCTAGC | 1 cut(s) | 2030 |
| AvaII | GGWCC | 1 cut(s) | 1904 |
| BanII | GRGCYC | 1 cut(s) | 2430 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2006, 2038 |
| BbsI | GAAGAC | 2 cut(s) | 608, 2074 |
| BbvI | GCAGC | 3 cut(s) | 100, 1378, 1722 |
| BccI | CCATC | 1 cut(s) | 1915 |
| BceAI | ACGGC | 1 cut(s) | 330 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2362, 2396 |
| BciT130I | CCWGG | 2 cut(s) | 1854, 1902 |
| BclI | TGATCA | 4 cut(s) | 445, 655, 775, 1878 |
| BcnI | CCSGG | 2 cut(s) | 1712, 2430 |
| BcoDI | GTCTC | 2 cut(s) | 1301, 2209 |
| BfaI | CTAG | 4 cut(s) | 1509, 2031, 2190, 2291 |
| BfoI | RGCGCY | 1 cut(s) | 2037 |
| BfuAI | ACCTGC | 1 cut(s) | 1930 |
| BglII | AGATCT | 1 cut(s) | 2211 |
| BisI | GCNGC | 3 cut(s) | 114, 1392, 1736 |
| BlsI | GCNGC | 3 cut(s) | 115, 1393, 1737 |
| Bme1390I | CCNGG | 4 cut(s) | 1712, 1854, 1902, 2430 |
| Bme18I | GGWCC | 1 cut(s) | 1904 |
| BmgT120I | GGNCC | 4 cut(s) | 340, 550, 883, 1904 |
| BmiI | GGNNCC | 3 cut(s) | 405, 735, 2427 |
| BmrFI | CCNGG | 4 cut(s) | 1712, 1854, 1902, 2430 |
| BmrI | ACTGGG | 1 cut(s) | 1778 |
| BmsI | GCATC | 5 cut(s) | 265, 475, 808, 1513, 1570 |
| BmtI | GCTAGC | 1 cut(s) | 2034 |
| BmuI | ACTGGG | 1 cut(s) | 1778 |
| BpiI | GAAGAC | 2 cut(s) | 608, 2074 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1560 |
| Bpu14I | TTCGAA | 1 cut(s) | 1924 |
| BpuEI | CTTGAG | 2 cut(s) | 1573, 2102 |
| BpuMI | CCSGG | 2 cut(s) | 1712, 2430 |
| Bsa29I | ATCGAT | 1 cut(s) | 1632 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1890, 2216 |
| BsaHI | GRCGYC | 1 cut(s) | 2109 |
| BsaJI | CCNNGG | 4 cut(s) | 312, 407, 855, 953 |
| BsaWI | WCCGGW | 1 cut(s) | 992 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 1611, 2167, 2264, 2338 |
| Bse1I | ACTGG | 2 cut(s) | 1773, 2144 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1890, 2216 |
| BseBI | CCWGG | 2 cut(s) | 1854, 1902 |
| BseCI | ATCGAT | 1 cut(s) | 1632 |
| BseDI | CCNNGG | 4 cut(s) | 312, 407, 855, 953 |
| BseGI | GGATG | 6 cut(s) | 97, 308, 361, 851, 976, 1364 |
| BseJI | GATNNNNATC | 2 cut(s) | 1890, 2216 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 1611, 2167, 2264, 2338 |
| BseMII | CTCAG | 4 cut(s) | 654, 1142, 2176, 2220 |
| BseNI | ACTGG | 2 cut(s) | 1773, 2144 |
| BsePI | GCGCGC | 1 cut(s) | 2385 |
| BseRI | GAGGAG | 8 cut(s) | 395, 605, 725, 938, 941, 1298, 1394, 2354 |
| BseXI | GCAGC | 3 cut(s) | 100, 1378, 1722 |
| Bsh1236I | CGCG | 1 cut(s) | 2387 |
| BshFI | GGCC | 6 cut(s) | 272, 342, 552, 884, 2092, 2160 |
| BshVI | ATCGAT | 1 cut(s) | 1632 |
| BsiSI | CCGG | 3 cut(s) | 993, 1712, 2430 |
| BslFI | GGGAC | 1 cut(s) | 1719 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 1611, 2167, 2264, 2338 |
| BsmAI | GTCTC | 2 cut(s) | 1301, 2209 |
| BsmFI | GGGAC | 1 cut(s) | 1719 |
| BsnI | GGCC | 6 cut(s) | 272, 342, 552, 884, 2092, 2160 |
| Bsp119I | TTCGAA | 1 cut(s) | 1924 |
| Bsp1286I | GDGCHC | 1 cut(s) | 2430 |
| Bsp1407I | TGTACA | 1 cut(s) | 2362 |
| Bsp19I | CCATGG | 1 cut(s) | 855 |
| BspACI | CCGC | 2 cut(s) | 731, 1145 |
| BspANI | GGCC | 6 cut(s) | 272, 342, 552, 884, 2092, 2160 |
| BspCNI | CTCAG | 4 cut(s) | 655, 1143, 2175, 2219 |
| BspDI | ATCGAT | 1 cut(s) | 1632 |
| BspFNI | CGCG | 1 cut(s) | 2387 |
| BspHI | TCATGA | 2 cut(s) | 702, 1875 |
| BspLI | GGNNCC | 3 cut(s) | 405, 735, 2427 |
| BspMI | ACCTGC | 1 cut(s) | 1930 |
| BspOI | GCTAGC | 1 cut(s) | 2034 |
| BspPI | GGATC | 6 cut(s) | 44, 1215, 1797, 2171, 2353, 2387 |
| BspQI | GCTCTTC | 2 cut(s) | 942, 1956 |
| BspT104I | TTCGAA | 1 cut(s) | 1924 |
| BsrGI | TGTACA | 1 cut(s) | 2362 |
| BsrI | ACTGG | 2 cut(s) | 1773, 2144 |
| BssECI | CCNNGG | 4 cut(s) | 312, 407, 855, 953 |
| BssHII | GCGCGC | 1 cut(s) | 2385 |
| BssNAI | GTATAC | 1 cut(s) | 2243 |
| BssNI | GRCGYC | 1 cut(s) | 2109 |
| BssT1I | CCWWGG | 3 cut(s) | 407, 855, 953 |
| Bst1107I | GTATAC | 1 cut(s) | 2243 |
| Bst2UI | CCWGG | 2 cut(s) | 1854, 1902 |
| BstACI | GRCGYC | 1 cut(s) | 2109 |
| BstAUI | TGTACA | 1 cut(s) | 2362 |
| BstBI | TTCGAA | 1 cut(s) | 1924 |
| BstC8I | GCNNGC | 2 cut(s) | 2032, 2387 |
| BstDEI | CTNAG | 8 cut(s) | 663, 1151, 1560, 1667, 1911, 2162, 2206, 2421 |
| BstDSI | CCRYGG | 2 cut(s) | 312, 855 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2165 |
| BstF5I | GGATG | 6 cut(s) | 97, 308, 361, 851, 976, 1364 |
| BstFNI | CGCG | 1 cut(s) | 2387 |
| BstH2I | RGCGCY | 1 cut(s) | 2037 |
| BstHHI | GCGC | 3 cut(s) | 2036, 2387, 2389 |
| BstMAI | GTCTC | 2 cut(s) | 1301, 2209 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 110, 2027, 2261 |
| BstNI | CCWGG | 2 cut(s) | 1854, 1902 |
| BstNSI | RCATGY | 1 cut(s) | 1795 |
| BstSCI | CCNGG | 4 cut(s) | 1710, 1852, 1900, 2428 |
| BstUI | CGCG | 1 cut(s) | 2387 |
| BstV1I | GCAGC | 3 cut(s) | 100, 1378, 1722 |
| BstV2I | GAAGAC | 2 cut(s) | 608, 2074 |
| BstX2I | RGATCY | 2 cut(s) | 1802, 2211 |
| BstYI | RGATCY | 2 cut(s) | 1802, 2211 |
| BstZ17I | GTATAC | 1 cut(s) | 2243 |
| Bsu15I | ATCGAT | 1 cut(s) | 1632 |
| BsuRI | GGCC | 6 cut(s) | 272, 342, 552, 884, 2092, 2160 |
| BsuTUI | ATCGAT | 1 cut(s) | 1632 |
| BtgI | CCRYGG | 2 cut(s) | 312, 855 |
| BtsCI | GGATG | 6 cut(s) | 97, 308, 361, 851, 976, 1364 |
| BtsI | GCAGTG | 1 cut(s) | 165 |
| BtsIMutI | CAGTG | 2 cut(s) | 165, 2032 |
| BveI | ACCTGC | 1 cut(s) | 1930 |
| Cac8I | GCNNGC | 2 cut(s) | 2032, 2387 |
| CciI | TCATGA | 2 cut(s) | 702, 1875 |
| CfoI | GCGC | 3 cut(s) | 2036, 2387, 2389 |
| Cfr13I | GGNCC | 4 cut(s) | 340, 550, 883, 1904 |
| ClaI | ATCGAT | 1 cut(s) | 1632 |
| Csp6I | GTAC | 8 cut(s) | 288, 498, 831, 1496, 1766, 2014, 2122, 2363 |
| CviAII | CATG | 6 cut(s) | 703, 856, 1127, 1178, 1792, 1876 |
| CviQI | GTAC | 8 cut(s) | 288, 498, 831, 1496, 1766, 2014, 2122, 2363 |
| DdeI | CTNAG | 8 cut(s) | 663, 1151, 1560, 1667, 1911, 2162, 2206, 2421 |
| DraI | TTTAAA | 1 cut(s) | 21 |
| EciI | GGCGGA | 1 cut(s) | 1160 |
| Eco130I | CCWWGG | 3 cut(s) | 407, 855, 953 |
| Eco24I | GRGCYC | 1 cut(s) | 2430 |
| Eco47I | GGWCC | 1 cut(s) | 1904 |
| Eco47III | AGCGCT | 1 cut(s) | 2035 |
| Eco57I | CTGAAG | 6 cut(s) | 14, 75, 552, 627, 1797, 2008 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2165 |
| EcoRII | CCWGG | 2 cut(s) | 1852, 1900 |
| EcoT14I | CCWWGG | 3 cut(s) | 407, 855, 953 |
| EcoT38I | GRGCYC | 1 cut(s) | 2430 |
| ErhI | CCWWGG | 3 cut(s) | 407, 855, 953 |
| FaeI | CATG | 6 cut(s) | 706, 859, 1130, 1181, 1795, 1879 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1829, 1861 |
| FaqI | GGGAC | 1 cut(s) | 1719 |
| FatI | CATG | 6 cut(s) | 702, 855, 1126, 1177, 1791, 1875 |
| FauNDI | CATATG | 1 cut(s) | 2350 |
| FbaI | TGATCA | 4 cut(s) | 445, 655, 775, 1878 |
| FblI | GTMKAC | 1 cut(s) | 2242 |
| Fnu4HI | GCNGC | 3 cut(s) | 114, 1392, 1736 |
| FokI | GGATG | 6 cut(s) | 104, 295, 368, 838, 983, 1351 |
| FriOI | GRGCYC | 1 cut(s) | 2430 |
| Fsp4HI | GCNGC | 3 cut(s) | 114, 1392, 1736 |
| FspBI | CTAG | 4 cut(s) | 1509, 2031, 2190, 2291 |
| GlaI | GCGC | 3 cut(s) | 2035, 2386, 2388 |
| GluI | GCNGC | 3 cut(s) | 114, 1392, 1736 |
| HaeII | RGCGCY | 1 cut(s) | 2037 |
| HaeIII | GGCC | 6 cut(s) | 272, 342, 552, 884, 2092, 2160 |
| HapII | CCGG | 3 cut(s) | 993, 1712, 2430 |
| HhaI | GCGC | 3 cut(s) | 2036, 2387, 2389 |
| Hin1I | GRCGYC | 1 cut(s) | 2109 |
| Hin1II | CATG | 6 cut(s) | 706, 859, 1130, 1181, 1795, 1879 |
| Hin6I | GCGC | 3 cut(s) | 2034, 2385, 2387 |
| HinP1I | GCGC | 3 cut(s) | 2034, 2385, 2387 |
| HincII | GTYRAC | 1 cut(s) | 2445 |
| HindII | GTYRAC | 1 cut(s) | 2445 |
| HinfI | GANTC | 3 cut(s) | 1058, 1634, 2100 |
| HpaI | GTTAAC | 1 cut(s) | 2445 |
| HpaII | CCGG | 3 cut(s) | 993, 1712, 2430 |
| HphI | GGTGA | 5 cut(s) | 385, 595, 928, 1670, 2037 |
| Hpy166II | GTNNAC | 4 cut(s) | 1576, 2243, 2311, 2445 |
| Hpy188I | TCNGA | 9 cut(s) | 33, 1057, 1114, 1836, 2165, 2209, 2236, 2405, 2511 |
| Hpy8I | GTNNAC | 4 cut(s) | 1576, 2243, 2311, 2445 |
| HpyCH4IV | ACGT | 1 cut(s) | 2109 |
| HpyCH4V | TGCA | 5 cut(s) | 83, 104, 1394, 1484, 1982 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 110, 2027, 2261 |
| HpyF3I | CTNAG | 8 cut(s) | 663, 1151, 1560, 1667, 1911, 2162, 2206, 2421 |
| HpySE526I | ACGT | 1 cut(s) | 2109 |
| Hsp92I | GRCGYC | 1 cut(s) | 2109 |
| Hsp92II | CATG | 6 cut(s) | 706, 859, 1130, 1181, 1795, 1879 |
| HspAI | GCGC | 3 cut(s) | 2034, 2385, 2387 |
| Ksp22I | TGATCA | 4 cut(s) | 445, 655, 775, 1878 |
| KspAI | GTTAAC | 1 cut(s) | 2445 |
| LguI | GCTCTTC | 2 cut(s) | 942, 1956 |
| LmnI | GCTCC | 5 cut(s) | 1147, 1918, 2370, 2425, 2459 |
| Lsp1109I | GCAGC | 3 cut(s) | 100, 1378, 1722 |
| LweI | GCATC | 5 cut(s) | 265, 475, 808, 1513, 1570 |
| MaeI | CTAG | 4 cut(s) | 1509, 2031, 2190, 2291 |
| MaeII | ACGT | 1 cut(s) | 2109 |
| MaeIII | GTNAC | 3 cut(s) | 1718, 1787, 2486 |
| MflI | RGATCY | 2 cut(s) | 1802, 2211 |
| MhlI | GDGCHC | 1 cut(s) | 2430 |
| MlyI | GAGTC | 1 cut(s) | 2109 |
| MmeI | TCCRAC | 2 cut(s) | 1018, 1392 |
| MseI | TTAA | 7 cut(s) | 20, 1088, 1193, 1595, 1731, 1848, 2444 |
| MspA1I | CMGCKG | 1 cut(s) | 1436 |
| MspI | CCGG | 3 cut(s) | 993, 1712, 2430 |
| MspR9I | CCNGG | 4 cut(s) | 1712, 1854, 1902, 2430 |
| MvaI | CCWGG | 2 cut(s) | 1854, 1902 |
| MvnI | CGCG | 1 cut(s) | 2387 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 110, 2027, 2261 |
| NciI | CCSGG | 2 cut(s) | 1712, 2430 |
| NcoI | CCATGG | 1 cut(s) | 855 |
| NdeI | CATATG | 1 cut(s) | 2350 |
| NheI | GCTAGC | 1 cut(s) | 2030 |
| NlaIII | CATG | 6 cut(s) | 706, 859, 1130, 1181, 1795, 1879 |
| NlaIV | GGNNCC | 3 cut(s) | 405, 735, 2427 |
| NmeAIII | GCCGAG | 1 cut(s) | 2068 |
| NspI | RCATGY | 1 cut(s) | 1795 |
| NspV | TTCGAA | 1 cut(s) | 1924 |
| PagI | TCATGA | 2 cut(s) | 702, 1875 |
| PaqCI | CACCTGC | 1 cut(s) | 1930 |
| PauI | GCGCGC | 1 cut(s) | 2385 |
| PciI | ACATGT | 1 cut(s) | 1791 |
| PciSI | GCTCTTC | 2 cut(s) | 942, 1956 |
| PfeI | GAWTC | 2 cut(s) | 1058, 1634 |
| PkrI | GCNGC | 3 cut(s) | 115, 1393, 1737 |
| PleI | GAGTC | 1 cut(s) | 2108 |
| PpsI | GAGTC | 1 cut(s) | 2108 |
| PscI | ACATGT | 1 cut(s) | 1791 |
| PsiI | TTATAA | 1 cut(s) | 1799 |
| Psp6I | CCWGG | 2 cut(s) | 1852, 1900 |
| PspGI | CCWGG | 2 cut(s) | 1852, 1900 |
| PspN4I | GGNNCC | 3 cut(s) | 405, 735, 2427 |
| PspPI | GGNCC | 4 cut(s) | 340, 550, 883, 1904 |
| PsuI | RGATCY | 2 cut(s) | 1802, 2211 |
| PteI | GCGCGC | 1 cut(s) | 2385 |
| PvuII | CAGCTG | 1 cut(s) | 1436 |
| RsaI | GTAC | 8 cut(s) | 289, 499, 832, 1497, 1767, 2015, 2123, 2364 |
| RsaNI | GTAC | 8 cut(s) | 288, 498, 831, 1496, 1766, 2014, 2122, 2363 |
| SapI | GCTCTTC | 2 cut(s) | 942, 1956 |
| SaqAI | TTAA | 7 cut(s) | 20, 1088, 1193, 1595, 1731, 1848, 2444 |
| SatI | GCNGC | 3 cut(s) | 114, 1392, 1736 |
| Sau96I | GGNCC | 4 cut(s) | 340, 550, 883, 1904 |
| SchI | GAGTC | 1 cut(s) | 2109 |
| ScrFI | CCNGG | 4 cut(s) | 1712, 1854, 1902, 2430 |
| SduI | GDGCHC | 1 cut(s) | 2430 |
| SfaNI | GCATC | 5 cut(s) | 265, 475, 808, 1513, 1570 |
| SfuI | TTCGAA | 1 cut(s) | 1924 |
| SinI | GGWCC | 1 cut(s) | 1904 |
| SmlI | CTYRAG | 2 cut(s) | 1552, 2117 |
| SmoI | CTYRAG | 2 cut(s) | 1552, 2117 |
| SsiI | CCGC | 2 cut(s) | 731, 1145 |
| SspI | AATATT | 1 cut(s) | 1872 |
| SspMI | CTAG | 4 cut(s) | 1509, 2031, 2190, 2291 |
| StyD4I | CCNGG | 4 cut(s) | 1710, 1852, 1900, 2428 |
| StyI | CCWWGG | 3 cut(s) | 407, 855, 953 |
| TaiI | ACGT | 1 cut(s) | 2112 |
| TaqI | TCGA | 6 cut(s) | 1157, 1632, 1637, 1842, 1924, 2451 |
| TatI | WGTACW | 2 cut(s) | 2121, 2362 |
| TfiI | GAWTC | 2 cut(s) | 1058, 1634 |
| Tru1I | TTAA | 7 cut(s) | 20, 1088, 1193, 1595, 1731, 1848, 2444 |
| Tru9I | TTAA | 7 cut(s) | 20, 1088, 1193, 1595, 1731, 1848, 2444 |
| TscAI | CASTG | 2 cut(s) | 165, 2032 |
| TseI | GCWGC | 3 cut(s) | 113, 1391, 1735 |
| TspDTI | ATGAA | 9 cut(s) | 201, 297, 507, 719, 840, 1194, 1761, 1864, 1943 |
| TspGWI | ACGGA | 2 cut(s) | 416, 635 |
| TspRI | CASTG | 2 cut(s) | 165, 2032 |
| VpaK11BI | GGWCC | 1 cut(s) | 1904 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2165 |
| XapI | RAATTY | 4 cut(s) | 1547, 2075, 2324, 2481 |
| XceI | RCATGY | 1 cut(s) | 1795 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 205 |
| XmiI | GTMKAC | 1 cut(s) | 2242 |
| XspI | CTAG | 4 cut(s) | 1509, 2031, 2190, 2291 |
| ZraI | GACGTC | 1 cut(s) | 2110 |